AR080340A1 - Plantas que tienen mejores rasgos relacionados con el rendimiento y un metodo para producirlas mediante la modulacion de la expresion de sgt-1, clcpkg o un polipeptido tipo hd-hidrolasa - Google Patents
Plantas que tienen mejores rasgos relacionados con el rendimiento y un metodo para producirlas mediante la modulacion de la expresion de sgt-1, clcpkg o un polipeptido tipo hd-hidrolasaInfo
- Publication number
- AR080340A1 AR080340A1 ARP100103484A AR080340A1 AR 080340 A1 AR080340 A1 AR 080340A1 AR P100103484 A ARP100103484 A AR P100103484A AR 080340 A1 AR080340 A1 AR 080340A1
- Authority
- AR
- Argentina
- Prior art keywords
- plants
- polypeptide
- expression
- performance
- clcpkg
- Prior art date
Links
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
- C12N15/8262—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield involving plant development
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
- C12N15/8262—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield involving plant development
- C12N15/827—Flower development or morphology, e.g. flowering promoting factor [FPF]
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
- C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
- C12N15/8273—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for drought, cold, salt resistance
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
- Y02A40/146—Genetically Modified [GMO] plants, e.g. transgenic plants
Landscapes
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Biophysics (AREA)
- Cell Biology (AREA)
- Physics & Mathematics (AREA)
- Plant Pathology (AREA)
- Medicinal Chemistry (AREA)
- Physiology (AREA)
- Botany (AREA)
- Gastroenterology & Hepatology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
Un método para mejorar rasgos relacionados con el rendimiento en plantas mediante la modulacion de la expresion en una planta de un ácido nucleico que codifica un polipéptido SGT1, o un polipéptido CLC-pKG o un polipéptido tipo HD-hidrolasa. También plantas que tienen expresion modulada de un ácido nucleico que codifica un polipéptido SGT1, un polipéptido CLC-pKG o un polipéptido tipo HD-hidrolasa, en donde dichas plantas tienen mejores rasgos relacionados con el rendimiento, con respecto a las correspondientes plantas de tipo silvestre u otras plantas de control. También constructos utiles en los métodos de la solicitud. Plantas y partes de plantas.
Applications Claiming Priority (6)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP09171385 | 2009-09-25 | ||
| EP09171364 | 2009-09-25 | ||
| US25222009P | 2009-10-16 | 2009-10-16 | |
| US25221509P | 2009-10-16 | 2009-10-16 | |
| US26094209P | 2009-11-13 | 2009-11-13 | |
| EP09175989 | 2009-11-13 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| AR080340A1 true AR080340A1 (es) | 2012-04-04 |
Family
ID=43242882
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ARP100103484 AR080340A1 (es) | 2009-09-25 | 2010-09-24 | Plantas que tienen mejores rasgos relacionados con el rendimiento y un metodo para producirlas mediante la modulacion de la expresion de sgt-1, clcpkg o un polipeptido tipo hd-hidrolasa |
Country Status (12)
| Country | Link |
|---|---|
| US (2) | US9371537B2 (es) |
| EP (2) | EP3118320A1 (es) |
| KR (1) | KR101754083B1 (es) |
| CN (1) | CN102648282A (es) |
| AR (1) | AR080340A1 (es) |
| AU (1) | AU2010299857A1 (es) |
| CA (1) | CA2773879A1 (es) |
| DE (1) | DE112010003793T5 (es) |
| EA (1) | EA201290168A1 (es) |
| MX (1) | MX338065B (es) |
| PH (1) | PH12012500517A1 (es) |
| WO (1) | WO2011036232A1 (es) |
Families Citing this family (17)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE112010003738T5 (de) * | 2009-10-22 | 2013-08-14 | Basf Plant Science Company Gmbh | Pflanzen mit gesteigerten Ertragsmerkmalen und Verfahren zu deren Herstellung |
| GB201122458D0 (en) | 2011-12-30 | 2012-02-08 | Univ Wageningen | Modified cascade ribonucleoproteins and uses thereof |
| SG11201507378UA (en) | 2013-03-14 | 2015-10-29 | Caribou Biosciences Inc | Compositions and methods of nucleic acid-targeting nucleic acids |
| US10760064B2 (en) | 2013-03-15 | 2020-09-01 | The General Hospital Corporation | RNA-guided targeting of genetic and epigenomic regulatory proteins to specific genomic loci |
| US10378027B2 (en) | 2013-03-15 | 2019-08-13 | The General Hospital Corporation | RNA-guided targeting of genetic and epigenomic regulatory proteins to specific genomic loci |
| WO2014204578A1 (en) | 2013-06-21 | 2014-12-24 | The General Hospital Corporation | Using rna-guided foki nucleases (rfns) to increase specificity for rna-guided genome editing |
| KR20260047646A (ko) * | 2013-07-09 | 2026-04-08 | 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 | 멀티플렉스 rna-가이드된 게놈 조작 |
| CN106459995B (zh) | 2013-11-07 | 2020-02-21 | 爱迪塔斯医药有限公司 | 使用统治型gRNA的CRISPR相关方法和组合物 |
| CN107074919A (zh) * | 2014-06-25 | 2017-08-18 | 菲利普莫里斯产品有限公司 | 通过氯化物通道的异位表达调节植物中的生物质 |
| US9512446B1 (en) | 2015-08-28 | 2016-12-06 | The General Hospital Corporation | Engineered CRISPR-Cas9 nucleases |
| US9926546B2 (en) | 2015-08-28 | 2018-03-27 | The General Hospital Corporation | Engineered CRISPR-Cas9 nucleases |
| CN107641153B (zh) * | 2017-11-16 | 2020-10-30 | 河南农业大学 | 干旱条件下小麦低温胁迫响应蛋白TaCDCP及其编码基因和应用 |
| CN110619131B (zh) * | 2018-06-20 | 2022-11-29 | 陕西师范大学 | 一种基于加权模糊Petri网的UUV智能体行为规划方法 |
| CN108950019A (zh) * | 2018-08-25 | 2018-12-07 | 潍坊德诺泰克生物科技有限公司 | 肝簇虫的Real-time PCR特异性引物和探针及检测试剂盒与检测方法 |
| CN111363752B (zh) * | 2020-04-29 | 2022-05-20 | 海南大学 | 一种橡胶树白粉病抗性相关基因HbSGT1b及其应用 |
| CN119955811B (zh) * | 2025-02-28 | 2026-02-24 | 济南大学 | 一种小麦耐盐基因TaSGT1及其应用 |
| CN120060346A (zh) * | 2025-03-06 | 2025-05-30 | 山东农业大学 | La6基因在调控水稻分蘖角度中的应用 |
Family Cites Families (32)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4962028A (en) | 1986-07-09 | 1990-10-09 | Dna Plant Technology Corporation | Plant promotors |
| US4987071A (en) | 1986-12-03 | 1991-01-22 | University Patents, Inc. | RNA ribozyme polymerases, dephosphorylases, restriction endoribonucleases and methods |
| US5004863B2 (en) | 1986-12-03 | 2000-10-17 | Agracetus | Genetic engineering of cotton plants and lines |
| US5116742A (en) | 1986-12-03 | 1992-05-26 | University Patents, Inc. | RNA ribozyme restriction endoribonucleases and methods |
| WO1989012102A1 (en) | 1988-06-01 | 1989-12-14 | The Texas A&M University System | Method for transforming plants via the shoot apex |
| AU4115693A (en) | 1992-04-24 | 1993-11-29 | Sri International | In vivo homologous sequence targeting in eukaryotic cells |
| ATE312510T1 (de) | 1992-06-29 | 2005-12-15 | Gene Shears Pty Ltd | Nukleinsäuren und sie verwendende verfahren zur bekämpfung viraler pathogene |
| US5401836A (en) | 1992-07-16 | 1995-03-28 | Pioneer Hi-Bre International, Inc. | Brassica regulatory sequence for root-specific or root-abundant gene expression |
| WO1994012015A1 (en) | 1992-11-30 | 1994-06-09 | Chua Nam Hai | Expression motifs that confer tissue- and developmental-specific expression in plants |
| JPH09505461A (ja) | 1993-07-22 | 1997-06-03 | ジーン シェアーズ プロプライアタリー リミティド | Dnaウィルスリボザイム |
| KR960705939A (ko) | 1993-11-19 | 1996-11-08 | 제이. 그랜트 브리븐 | 식물의 유전자 발현을 위한 키메라 조절부위 및 유전자 카세트 |
| CN1048254C (zh) | 1993-12-09 | 2000-01-12 | 托马斯杰弗逊大学 | 用于将预定的改变引入靶基因中的化合物 |
| US5605793A (en) | 1994-02-17 | 1997-02-25 | Affymax Technologies N.V. | Methods for in vitro recombination |
| US6395547B1 (en) | 1994-02-17 | 2002-05-28 | Maxygen, Inc. | Methods for generating polynucleotides having desired characteristics by iterative selection and recombination |
| EP0853676B1 (en) | 1995-10-06 | 2006-06-21 | Bayer BioScience N.V. | Seed shattering |
| US7390937B2 (en) | 1996-02-14 | 2008-06-24 | The Governors Of The University Of Alberta | Plants with enhanced levels of nitrogen utilization proteins in their root epidermis and uses thereof |
| GB9607517D0 (en) | 1996-04-11 | 1996-06-12 | Gene Shears Pty Ltd | The use of DNA Sequences |
| GB9703146D0 (en) | 1997-02-14 | 1997-04-02 | Innes John Centre Innov Ltd | Methods and means for gene silencing in transgenic plants |
| GB9710475D0 (en) | 1997-05-21 | 1997-07-16 | Zeneca Ltd | Gene silencing |
| GB9720148D0 (en) | 1997-09-22 | 1997-11-26 | Innes John Centre Innov Ltd | Gene silencing materials and methods |
| AU760041B2 (en) | 1998-04-08 | 2003-05-08 | Commonwealth Scientific And Industrial Research Organisation | Methods and means for obtaining modified phenotypes |
| BR9911540A (pt) | 1998-06-26 | 2001-11-27 | Univ Iowa State Res Found Inc | Materiais e métodos para a alteração dos nìveis deenzima e de acetil coa em plantas |
| EP0982404A1 (en) * | 1998-08-27 | 2000-03-01 | Wallaart, Thorvald Eelco | DNA encoding amorpha-4,11-diene synthase |
| US6555732B1 (en) | 1998-09-14 | 2003-04-29 | Pioneer Hi-Bred International, Inc. | Rac-like genes and methods of use |
| CN1279172C (zh) | 1999-07-22 | 2006-10-11 | 独立行政法人农业生物资源研究所 | 转化水稻植物的方法 |
| PL354490A1 (en) | 1999-08-26 | 2004-01-26 | Basf Aktiengesellschaft | Plant gene expression, controlled by constitutive plant v-atpase promoters |
| US7235710B2 (en) | 2003-01-21 | 2007-06-26 | Cropdesign N.V. | Regulatory sequence |
| CN1748032B (zh) | 2003-02-04 | 2012-07-18 | 作物培植股份有限公司 | 稻启动子 |
| CA2516042A1 (en) * | 2003-02-17 | 2004-09-02 | Metanomics Gmbh | Preparation of transgenic plants comprising a nucleic acid molecule encoding an l450 polypeptide-exhibiting faster growth and/or increased yield |
| WO2005113777A1 (en) | 2004-05-21 | 2005-12-01 | MAX-PLANCK-Gesellschaft zur Förderung der Wissenschaften e.V. | Means and methods for producing stress-resistant plants |
| EP1820391A1 (en) | 2006-02-17 | 2007-08-22 | CropDesign N.V. | Method and apparatus to determine the start of flowering in plants |
| CN101415829B (zh) * | 2006-03-31 | 2014-03-12 | 巴斯福植物科学有限公司 | 具有增强的产量相关性状的植物和用于产生该植物的方法 |
-
2010
- 2010-09-24 AU AU2010299857A patent/AU2010299857A1/en not_active Abandoned
- 2010-09-24 MX MX2012003402A patent/MX338065B/es active IP Right Grant
- 2010-09-24 DE DE201011003793 patent/DE112010003793T5/de not_active Withdrawn
- 2010-09-24 US US13/498,003 patent/US9371537B2/en not_active Expired - Fee Related
- 2010-09-24 EP EP16179077.9A patent/EP3118320A1/en not_active Withdrawn
- 2010-09-24 EA EA201290168A patent/EA201290168A1/ru unknown
- 2010-09-24 CA CA 2773879 patent/CA2773879A1/en not_active Abandoned
- 2010-09-24 EP EP10768424A patent/EP2480674A1/en not_active Withdrawn
- 2010-09-24 CN CN2010800533114A patent/CN102648282A/zh active Pending
- 2010-09-24 PH PH1/2012/500517A patent/PH12012500517A1/en unknown
- 2010-09-24 KR KR1020127010589A patent/KR101754083B1/ko not_active Expired - Fee Related
- 2010-09-24 AR ARP100103484 patent/AR080340A1/es unknown
- 2010-09-24 WO PCT/EP2010/064095 patent/WO2011036232A1/en not_active Ceased
-
2015
- 2015-09-08 US US14/847,605 patent/US20160002657A1/en not_active Abandoned
Also Published As
| Publication number | Publication date |
|---|---|
| US9371537B2 (en) | 2016-06-21 |
| MX2012003402A (es) | 2012-04-30 |
| KR101754083B1 (ko) | 2017-07-05 |
| AU2010299857A1 (en) | 2012-04-19 |
| CA2773879A1 (en) | 2011-03-31 |
| AU2010299857A2 (en) | 2013-05-16 |
| EA201290168A1 (ru) | 2013-10-30 |
| US20160002657A1 (en) | 2016-01-07 |
| PH12012500517A1 (en) | 2012-11-12 |
| CN102648282A (zh) | 2012-08-22 |
| US20130145497A1 (en) | 2013-06-06 |
| EP2480674A1 (en) | 2012-08-01 |
| EP3118320A1 (en) | 2017-01-18 |
| MX338065B (es) | 2016-04-01 |
| KR20120125225A (ko) | 2012-11-14 |
| WO2011036232A1 (en) | 2011-03-31 |
| DE112010003793T5 (de) | 2012-11-22 |
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