BR9911540A - Materiais e métodos para a alteração dos nìveis deenzima e de acetil coa em plantas - Google Patents
Materiais e métodos para a alteração dos nìveis deenzima e de acetil coa em plantasInfo
- Publication number
- BR9911540A BR9911540A BR9911540-9A BR9911540A BR9911540A BR 9911540 A BR9911540 A BR 9911540A BR 9911540 A BR9911540 A BR 9911540A BR 9911540 A BR9911540 A BR 9911540A
- Authority
- BR
- Brazil
- Prior art keywords
- plant
- ppdh
- dehydrogenase
- enzyme
- acetyl coa
- Prior art date
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Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
- C12N15/1137—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing against enzymes
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
- C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/0008—Oxidoreductases (1.) acting on the aldehyde or oxo group of donors (1.2)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/0051—Oxidoreductases (1.) acting on a sulfur group of donors (1.8)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/1025—Acyltransferases (2.3)
- C12N9/1029—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/88—Lyases (4.)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/93—Ligases (6)
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- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- General Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Plant Pathology (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Virology (AREA)
- Nutrition Science (AREA)
- Cell Biology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Enzymes And Modification Thereof (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
"MATERIAIS E MéTODOS PARA A ALTERAçãO DOS NìVEISDE ENZIMA E DE ACETIL CoA EM PLANTAS". A presenteinvenção provê sequências de ácido nucléico e aminoácido desintetase de acetil CoA (ACS) , dehidrogenase de piruvatoplastídico (PPDH), liase de ATP-citrato (ACL), decarboxilase depiruvato Arabidopsis (PDC), e dehidrogenase de aldeídoArabidopsis (ALDH), especificamente ALDH-2 e ALDH-4. Apresente invenção também provê um vetor recombinante quecompreende uma seq³ência de ácido nucléico que codifica umadas enzimas acima mencionadas, uma seq³ência anti-percepçãoou uma ribozima, uma célula transformada com um vetor,anticorpos para as enzimas, uma célula de planta, um tecido deplanta, um organismo de planta, ou uma planta nas quais o nivel deuma enzima foi alterado, e um método de produção de tais célulade planta, tecido de planta, organismo de planta, ou planta. Emadição, a presente invenção provê um vetor recombinante quecompreende uma seq³ência anti-percepção de uma seq³ência deácido nucléico que codifica decarboxilase de piruvato (PDC), asub-unidade E1<244> do pPDH, a sub-unidade E1<225> dopPDH, a sub-unidade E2 do pPDH, dehidrogenase de piruvatomitocondrial (mtPDH) ou dehidrogenase de aldeído (ALDH) ou umaribozima que pode quebrar uma molécula de RNA que codificaPDC, E1<244>pPDH, E1<225>pPDH, E2pPDH, mtPDH ou ALDH.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US9071798P | 1998-06-26 | 1998-06-26 | |
PCT/US1999/014382 WO2000000619A2 (en) | 1998-06-26 | 1999-06-25 | MATERIALS AND METHODS FOR THE ALTERATION OF ENZYME AND ACETYL CoA LEVELS IN PLANTS |
Publications (1)
Publication Number | Publication Date |
---|---|
BR9911540A true BR9911540A (pt) | 2001-11-27 |
Family
ID=22223980
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
BR9911540-9A BR9911540A (pt) | 1998-06-26 | 1999-06-25 | Materiais e métodos para a alteração dos nìveis deenzima e de acetil coa em plantas |
Country Status (9)
Country | Link |
---|---|
US (3) | US6764851B2 (pt) |
EP (1) | EP1090124A2 (pt) |
CN (2) | CN1268749C (pt) |
AU (1) | AU756960B2 (pt) |
BR (1) | BR9911540A (pt) |
CA (1) | CA2331327A1 (pt) |
MX (1) | MXPA01000174A (pt) |
TR (1) | TR200100705T2 (pt) |
WO (1) | WO2000000619A2 (pt) |
Families Citing this family (85)
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EP2794888B1 (en) | 2011-12-22 | 2017-02-22 | E. I. du Pont de Nemours and Company | Use of the soybean sucrose synthase promoter to increase plant seed lipid content |
EP2677035A1 (en) | 2012-06-22 | 2013-12-25 | BASF Plant Science Company GmbH | Plants having enhanced yield-related traits and a method for making the same |
US10024856B2 (en) * | 2013-03-21 | 2018-07-17 | Baylor College Of Medicine | Identification and characterization of a peptide affinity reagent for the detection of noroviruses in clinical samples |
EP2896698A1 (en) | 2014-01-17 | 2015-07-22 | BASF Plant Science Company GmbH | Plants having one or more enhanced yield-related traits and a method for making the same |
WO2016164810A1 (en) | 2015-04-08 | 2016-10-13 | Metabolix, Inc. | Plants with enhanced yield and methods of construction |
CN111072759B (zh) * | 2018-10-18 | 2022-10-21 | 深圳市作物分子设计育种研究院 | 一种造粉体蛋白转运信号肽及其在花粉育性调控中的应用 |
CN115927453B (zh) * | 2023-01-31 | 2023-11-24 | 昆明理工大学 | 苹果酸脱氢酶基因在提高植物甲醛吸收代谢能力中的应用 |
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US5270175A (en) * | 1991-07-12 | 1993-12-14 | Dna Plant Technology Corporation | Methods and compositions for producing metabolic products for algae |
US5633153A (en) | 1994-10-14 | 1997-05-27 | Calgene, Inc. | Aldehyde dehydrogenase selectable markers for plant transformation |
US5684242A (en) | 1994-11-29 | 1997-11-04 | Iowa State University Research Foundation, Inc. | Nuclear restorer genes for hybrid seed production |
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ZA971885B (en) | 1996-03-05 | 1997-10-16 | Weissheimer Friedr Malzfab | Production of products from transgenic plant material. |
US5958745A (en) * | 1996-03-13 | 1999-09-28 | Monsanto Company | Methods of optimizing substrate pools and biosynthesis of poly-β-hydroxybutyrate-co-poly-β-hydroxyvalerate in bacteria and plants |
US5695973A (en) * | 1996-07-03 | 1997-12-09 | R.J. Reynolds Tobacco Company | Isolated alcohol dehydrogenase producing mold |
CA2263186A1 (en) * | 1996-08-08 | 1998-02-19 | Max-Planck-Gesellschaft Zur Forderung Der Wissenschaften E.V. | Transgenic plant cells and plants with modified acetyl-coa formation |
GB9710370D0 (en) * | 1997-05-20 | 1997-07-16 | Zeneca Ltd | Genetic control of fruit ripening |
EP0991768A1 (en) | 1997-05-22 | 2000-04-12 | Washington State University Research Foundation | Sucrose-binding proteins |
WO1999000505A1 (en) | 1997-06-30 | 1999-01-07 | The Curators Of The University Of Missouri | Use of dna encoding plastid pyruvate dehydrogenase and branched chain oxoacid dehydrogenase components to enhance polyhydroxyalkanoate biosynthesis in plants |
WO1999013067A2 (en) * | 1997-09-12 | 1999-03-18 | Purdue Research Foundation | Hypoxia inducible promoter |
AU5551599A (en) | 1998-08-20 | 2000-03-14 | Pioneer Hi-Bred International, Inc. | Compositions and methods for altering an acetyl-coa metabolic pathway of a plant |
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- 1999-06-25 CA CA002331327A patent/CA2331327A1/en not_active Abandoned
- 1999-06-25 US US09/344,882 patent/US6764851B2/en not_active Expired - Fee Related
- 1999-06-25 WO PCT/US1999/014382 patent/WO2000000619A2/en not_active Application Discontinuation
- 1999-06-25 TR TR2001/00705T patent/TR200100705T2/xx unknown
- 1999-06-25 EP EP99930711A patent/EP1090124A2/en not_active Withdrawn
- 1999-06-25 AU AU47192/99A patent/AU756960B2/en not_active Ceased
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US7524678B2 (en) | 2009-04-28 |
US6764851B2 (en) | 2004-07-20 |
CA2331327A1 (en) | 2000-01-06 |
US20020162137A1 (en) | 2002-10-31 |
US6942994B2 (en) | 2005-09-13 |
EP1090124A2 (en) | 2001-04-11 |
TR200100705T2 (tr) | 2001-10-22 |
US20030106090A1 (en) | 2003-06-05 |
CN1912118A (zh) | 2007-02-14 |
AU756960B2 (en) | 2003-01-30 |
MXPA01000174A (es) | 2003-09-10 |
AU4719299A (en) | 2000-01-17 |
CN1268749C (zh) | 2006-08-09 |
WO2000000619A3 (en) | 2000-06-15 |
WO2000000619A2 (en) | 2000-01-06 |
CN1322252A (zh) | 2001-11-14 |
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