BR112014002745A8 - produção de isoprenoides sob condições de ph neutro - Google Patents

produção de isoprenoides sob condições de ph neutro

Info

Publication number
BR112014002745A8
BR112014002745A8 BR112014002745A BR112014002745A BR112014002745A8 BR 112014002745 A8 BR112014002745 A8 BR 112014002745A8 BR 112014002745 A BR112014002745 A BR 112014002745A BR 112014002745 A BR112014002745 A BR 112014002745A BR 112014002745 A8 BR112014002745 A8 BR 112014002745A8
Authority
BR
Brazil
Prior art keywords
saccharification
fermentation
ph
process
isoprenoids
Prior art date
Application number
BR112014002745A
Other languages
English (en)
Other versions
BR112014002745A2 (pt
Inventor
R Calabria Anthony
A Strohm Bruce
Duan Gang
K Chotani Gopal
Xu Hongxian
K Shetty Jayarama
H Bergsma Martien
Johanna Mari Tuenissen Pauline
Ho Lee Sung
Sharma Vivek
A Cuevas William
Qian Ying
Original Assignee
Danisco Us Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority to US201161515830P priority Critical
Application filed by Danisco Us Inc filed Critical Danisco Us Inc
Priority to PCT/US2012/049646 priority patent/WO2013022799A1/en
Publication of BR112014002745A2 publication Critical patent/BR112014002745A2/pt
Publication of BR112014002745A8 publication Critical patent/BR112014002745A8/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P5/00—Preparation of hydrocarbons or halogenated hydrocarbons
    • C12P5/007—Preparation of hydrocarbons or halogenated hydrocarbons containing one or more isoprene units, i.e. terpenes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1085—Transferases (2.) transferring alkyl or aryl groups other than methyl groups (2.5)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411—Amylases
    • C12N9/2428—Glucan 1,4-alpha-glucosidase (3.2.1.3), i.e. glucoamylase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y205/00—Transferases transferring alkyl or aryl groups, other than methyl groups (2.5)
    • C12Y205/01—Transferases transferring alkyl or aryl groups, other than methyl groups (2.5) transferring alkyl or aryl groups, other than methyl groups (2.5.1)
    • C12Y205/0101—(2E,6E)-Farnesyl diphosphate synthase (2.5.1.10), i.e. geranyltranstransferase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01003—Glucan 1,4-alpha-glucosidase (3.2.1.3), i.e. glucoamylase

Abstract

abstract: en)embodiments of the present disclosure relate to a process for producing isoprenoid precursor molecules and/or isoprenoids from a starch substrate by saccharification and/or fermentation. the saccharification is effectively catalyzed by a glucoamylase at a ph in the range of 5.0 to 8.0. at a ph of 6.0 or above, the glucoamylase possesses at least 50% activity relative to its maximum activity. the saccharification and fermentation may be performed as a simultaneous saccharification and fermentation (ssf) process ------------------------------------------------------------------------------ tradução das reivindicações resumo patente de invenção: "produção de isoprenoides sob condições de ph neutro". modalidades da presente descrição referem-se a um processo para produzir moléculas precursoras de isoprenoide e/ou isoprenoides a partir de um substrato de amido por sacarificação e/ou fermentação. a sacarifi-cação é efetivamente catalisada por uma glicoamilase em um ph na faixa de 5,0 a 8,0. em um ph de 6,0 ou acima, a glicoamilase possui pelo menos 50% de atividade em relação a sua atividade máxima. os processos de sa-carificação e fermentação podem ser executados como um processo si-multâneo de sacarificação e fermentação (ssf).
BR112014002745A 2011-08-05 2012-08-03 produção de isoprenoides sob condições de ph neutro BR112014002745A8 (pt)

Priority Applications (2)

Application Number Priority Date Filing Date Title
US201161515830P true 2011-08-05 2011-08-05
PCT/US2012/049646 WO2013022799A1 (en) 2011-08-05 2012-08-03 PRODUCTION OF ISOPRENOIDS UNDER NEUTRAL pH CONDITIONS

Publications (2)

Publication Number Publication Date
BR112014002745A2 BR112014002745A2 (pt) 2017-06-13
BR112014002745A8 true BR112014002745A8 (pt) 2017-06-20

Family

ID=46724632

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112014002745A BR112014002745A8 (pt) 2011-08-05 2012-08-03 produção de isoprenoides sob condições de ph neutro

Country Status (5)

Country Link
US (1) US8951764B2 (pt)
EP (1) EP2739742A1 (pt)
BR (1) BR112014002745A8 (pt)
HK (1) HK1198837A1 (pt)
WO (1) WO2013022799A1 (pt)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103068997A (zh) * 2010-08-06 2013-04-24 丹尼斯科美国公司 中性pH糖化和发酵
EP2601300A1 (en) * 2010-08-06 2013-06-12 Danisco US Inc. Production of isoprene under neutral ph conditions

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Also Published As

Publication number Publication date
WO2013022799A1 (en) 2013-02-14
US20130203140A1 (en) 2013-08-08
EP2739742A1 (en) 2014-06-11
HK1198837A1 (zh) 2015-06-12
US8951764B2 (en) 2015-02-10
BR112014002745A2 (pt) 2017-06-13

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