CN102823628A - Honey bee attractant and method for seed production and honeybee pollination of soybean hybrid seed in field - Google Patents
Honey bee attractant and method for seed production and honeybee pollination of soybean hybrid seed in field Download PDFInfo
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- CN102823628A CN102823628A CN2012101838375A CN201210183837A CN102823628A CN 102823628 A CN102823628 A CN 102823628A CN 2012101838375 A CN2012101838375 A CN 2012101838375A CN 201210183837 A CN201210183837 A CN 201210183837A CN 102823628 A CN102823628 A CN 102823628A
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Abstract
The invention discloses a honey bee attractant and a method for seed production and pollination of a soybean hybrid seed in a field. The honey bee attractant is prepared from cellular liquid, imitation flower liquid, syrup and water. The method comprises the steps of: selecting the field suitable for growth of soybean, sowing the soybean hybrid seed in good time, interplanting a male parent and a female parent at an interval of a ridge, selecting required quantity of healthy pollination swarm before pollination, transporting the healthy pollination swarm in the soybean field at an early flowering stage of the soybean; feeding attractant syrup one hour before spraying the honey bee attractant in each day or at an interval of a day; forming 1-5 ridges for every1-3 days, spraying the honey bee attractant on hybrid soybean plant leaf of the male parent and the female parent in the 1-5 ridges; inducing a large quantity of bees to gradually fall on the hybrid soybean plant leaf to eat the attractant syrup; then spraying the honey bee attractant on the two sides of a hybrid soybean strain stem, inducing the bees to climb on the soybean strain stem and continue to climb on the soybean to collect, thereby pollinating the hybrid soybean; stopping spraying the honey bee attractant in a cloudy or rainy day, and spraying the honey bee attractant when the weather gets nicer, so as to keep enough attractant required for inducing the bees in the field.
Description
Technical field
The present invention relates to the honeybee attractant and be used for the method that soybean hybridizes the field seed production and pollination.
Background technology
Soybean is a self-pollinated plant, just can normally not bear pods through insect pollination.But hybrid soybean seeds must be solid through artificial pollination or insect pollination ability, and the Jilin Academy of Agricultural Science increases production more than 20% the raising the output 30～50% that has through the hybrid soybean seeds of seed selection for many years than common soybeans.Hybrid soybean seeds is that the soya cells matter male sterile line by different cultivars is maternal, and the soybean of different cultivars recovers system or maintenance line is a male parent, combines through hybridization.Yet the soybean hybridization pollination is the bottleneck of puzzlement hybrid soybean seeds field production of hybrid seeds key technology, and hybrid soybean seeds can not large tracts of land be promoted.Therefore solve the task of top priority that the soybean hybridization pollination has become the hybrid soybean seeds seeding technique.
Honeybee is outstanding Pollinating Insect, but honeybee dislikes interviewing big Tofu pudding, because soybean nectar, pollen are few, fragrance of a flower smell is low, and is little to the honeybee attraction, and therefore, to come be the hybrid soybean seeds pollination for the method for other crops pollination to use honeybee, and effect is undesirable.
Summary of the invention
The object of the invention is in order to solve the technical problem that exists in the production of hybrid seeds of hybrid soybean seeds field; A kind of honeybee attractant is provided and is used for hybrid soybean seeds field seed production and pollination method; To be suitable for the production of hybrid seeds of hybrid soybean seeds field, improve hybrid soybean seeds output, further apply in the production field.
Technical scheme of the present invention is: a kind of honeybee attractant, and with honeycomb liquid 7.0～15g, imitative fragrance of a flower liquid 2.5 ~ 5.0g, syrup 5.0 ~ 10g that the white sugar of water and equivalent makes, cold water 70～85.5g is mixed and forms; The preparation method of above-mentioned honeybee attractant is made up of following process and step.
(1) preparation of described honeycomb extract; Get honeybee nest spleen 1 ㎏, be cut into fragment, add 75% ethanol 1000ml; Airtight immersion 72h; After filtering with 100 order gauzes, it is for use to collect the broken honeycomb of filter residue, and it is filtrated and under 50 ℃-0.095Mpa vacuum, is concentrated into water content is 25% honeycomb liquid; Take the broken honeycomb after alcohol extract filters, add entry 1500ml, under 85～95 ℃ of stirrings, be concentrated to 800ml, stop heating, remove the beeswax that solidify on top after the cooling, filter with 100 order gauzes, waste is collected honeycomb liquid; The honeycomb liquid mixing of above-mentioned extracted twice is obtained honeycomb liquid.
(2) preparation of described imitative fragrance of a flower liquid; With the smart 2.5～5.0g of pollen, evening primrose oil 2.5～5.0g, orange oil 2.5～5.0g, perilla herb oil 2.5～5.0g mixing are contained in the bottle, add 50%～75% ethanol 80～90 ml again, and its mixing is formulated.
(3) get honeycomb liquid 7.0～15g, imitative fragrance of a flower liquid 2.5 ~ 5.0g, syrup 5.0 ~ 10g that the white sugar of water and equivalent makes, cold water 70～85.5g, mixing make described honeybee attractant.
2, the honeybee attractant is used for the method for hybrid soybean seeds field production of hybrid seeds honeybee pollination, is made up of following process and step:
(1) honeybee pollination hybrid soybean field is selected
According to regional climatic condition, select to be suitable for the field of soybeans they grow, by the normal field management hybrid soybean seeds of beginning sowing in good time, and implement to plant at a distance between ridge between male parent, the female parent.
(2) the pollination bee colony is selected
In the bee colony of normal breeding, select the pollination bee colony, the pollination bee colony will reach 8 frame honeybees, 7 crowd's gesture that sub-spleen is above; Wherein, it is several about 30% that foraging bee accounts for full crowd honeybee, and house bee accounts for about 70%; The healthy no swarming fever of bee colony, non-communicable disease.
(3) the pollination bee colony is transported soybeans
The pollination bee colony that the soybean blossoming initial stage will be selected is transported the hybrid soybean seeds seed farm, and per hectare is placed 3 ~ 6 groups in pollination bee colony.
(4) the honeybee attractant of spraying, feed
In between the hybrid soybean seeds seed farm of just having bloomed, every day or sprayed the attractant syrup of feeding preceding 1 hour implementing the honeybee attractant every other day; 1 ~ 5 ridge ground, per 1 ~ 3 day interval, the male parent in 1 ~ 5 ridge ground, hybridization of female parent soybean plant strain foliar spray mist honeybee attractant lure a large amount of honeybees to drop on the attractant syrup of on the hybrid soybean plants leaf, searching for food gradually; To hybrid soybean strain stalk two side spray honeybee attractants, lure honeybee to climb up on the soybean strain stalk again, climb to hybrid soybean successively and take collection, thereby be the hybrid soybean pollination; Fine normal spraying honeybee attractant, overcast and rainy stopping to be sprayed; Recover spraying honeybee attractant after weather takes a turn for the better at once, be enough to the satisfied needed attractant of honeybee of luring to keep the field to have.
(5) the soybean florescence finishes, the honeybee attractant that stops to spray, and the bee colony that will pollinate is withdrawn hybrid soybean production of hybrid seeds ground.
The present invention compared with prior art has following remarkable result:
1) to be used for luring the apis mellifera of raising or honeybee be that hybrid soybean seeds field seed production and pollination has been opened up a brand-new road to the honeybee attractant of the present invention exploitation.
2) the present invention can make the hybrid soybean seeds production of hybrid seeds rate of bearing pods reach (comparing with autophilous male parent) 78.9 % (low outcrossing rate kind)～87.8% (high outcrossing rate kind); Average every strain is born pods and is reached 52.9; The hybrid soybean seeds output that is applied in the production improves 17.7～49%; And advanced that hybrid soybean seeds is bred, the production of hybrid seeds and apply producing Tanaka, demonstrate obvious social and ecological benefits.
3) the honeybee attractant is researched and developed successfully, makes full use of honeybee and hybrid soybean seeds biological property separately, has brought into play the effect that cellular resources and imitative fragrance of a flower information are induced honeybee, makes the pollination honeybee be adapted to be hybrid soybean seeds field seed production and pollination, and is basic
OnBroken through the bottleneck of restriction hybrid soybean field seed production and pollination technology.
4) the honeybee attractant adopts the nonhazardous natural material, for the honeybee attractant is used in hybrid soybean field seed production and pollination, ripe complete honeybee attractant prescription and manufacturing condition thereof is provided, and efficient is high, and is pollution-free, to people and honeybee safety.
5) honeybee attractant first Application is pollinated between the hybrid soybean seeds seed farm; Solve honeybee and disliked interviewing big Tofu pudding; The difficult problem that pollination efficiency is low; Giving full play to the advantage of utilizing abundant honeybee resource pollination, is to hybrid soybean seeds seed selection, the land for growing field crops production of hybrid seeds, promotes the use of a revolution of soybean field planting.
Embodiment
In a large number
Embodiment 1: honeybee attractant and preparation method thereof
(1) honeybee attractant, with honeycomb liquid 7.0～15g, imitative fragrance of a flower liquid 2.5 ~ 5.0g, syrup 5.0 ~ 10g that the white sugar of water and equivalent makes, cold water 70～85.5g, being mixed forms,
(2) preparation of honeybee attractant, form by following process and step:
1) honeycomb liquid extracts: extract by following process and step:
1. get honeybee nest spleen 1 ㎏, be cut into fragment, add 75% ethanol 1000ml, airtight immersion 72h, after filtering with 100 order gauzes, collection filter residue honeycomb fragment, filtrating and under 50 ℃-0.095Mpa vacuum, being concentrated into water content is 25% honeycomb liquid.
2. take the honeycomb fragment after alcohol extract filters, add running water 1500ml, under 85～95 ℃ of stirrings, be concentrated to 800ml, stop heating, remove the beeswax of surface solidification after the cooling, filter with 100 order gauzes, waste is collected liquid.
3. the honeycomb liquid mixing that 1., 2. above-mentioned steps is extracted obtains honeycomb liquid.
2) allotment of imitative fragrance of a flower liquid: with the smart 2.5～5.0g of pollen, evening primrose oil 2.5～5.0g, orange oil 2.5～5.0g, perilla herb oil 2.5～5.0g mixing are contained in the bottle, add 50%～75% ethanol 80～90 ml again, with its mixing;
Embodiment 2: the honeybee attractant is used for the method for hybrid soybean seeds field seed production and pollination
1) select between honeybee pollination hybrid soybean seed farm:
According to regional climatic condition, select to be fit to the field of soybeans they grow, the hybrid soybean seeds of beginning sowing in good time; And implement normal field management; The rational application of fertilizer, pour water, insect protected, shovel time etc., guarantee that soybean is not influenced by damage by disease and insect, severe drought etc., keep the normal growing way of soybean.The production of hybrid seeds of hybrid soybean seeds field is according to planting between 1 ridge male parent → 1 ridge or the maternal separated ridge of 2 ridges, and promptly 1 ridge male parent → 1 ridge or 2 ridges female parent → 1 ridge male parent → 1 ridge or 2 ridges are maternal, by that analogy.
2) the pollination bee colony is selected:
Before pollination 5 ~ 7 days, in the bee colony of normal breeding, select the pollination bee colony, the pollination bee colony will reach 8 frame honeybees, 7 crowd's gesture that sub-spleen is above; Wherein, it is several about 30% that foraging bee accounts for full crowd honeybee, and house bee accounts for about 70%; The healthy no swarming fever of bee colony, non-communicable disease.
3) the pollination bee colony that will select is transported the hybrid soybean seeds seed farm soybean blossoming initial stage, and per hectare ground is placed 3～6 groups.
4) implement spraying, the honeybee attractant of feeding:, every day or every other day preceding about 1 hour of spraying honeybee attractant, be the bee colony attractant syrup of feeding in the field of the hybrid soybean seeds production of hybrid seeds of blooming; Spraying honeybee attractant on the hybrid soybean plants leaf then, per 1～3 day 1～5 ridge spraying honeybee attractant, 1～5 ridge at interval lures a large amount of honeybees to drop on the attractant syrup of on the hybrid soybean plants leaf, searching for food gradually; To hybrid soybean strain stalk two side spray honeybee attractants, lure honeybee to climb up on the soybean strain stalk again, climb to hybrid soybean successively again and take collection, thereby be the hybrid soybean pollination; Fine normal spraying honeybee attractant, overcast and rainy stopping to be sprayed; Recover spraying honeybee attractant after weather takes a turn for the better at once, be enough to satisfied attractant of luring honeybee to keep the field to have.
5) the soybean florescence finishes, and stops using attractant, and the bee colony that will pollinate is withdrawn soybeans.
6) survey bear pods rate and real output: the rate that bears pods is surveyed in soybean fallen leaves back, looks into maternal and the male parent quantity that bears pods, the calculating female parent rate that bears pods; Calculate the actual production of hybrid soybean seeds father, female parent during soybean maturation results respectively.
The present invention utilizes honeybee to be the hybrid soybean seeds pollination technique, the honeycomb liquid of selecting for use the different cultivars honeybee to utilize to contain in the honeybee attractant, imitative fragrance of a flower liquid and syrup have certain attraction to honeybee, has transferred the enthusiasm that honeybee is interviewed big Tofu pudding.Select to be fit to the field planting soybean crossbreed of soybeans they grow, broken through through the spraying attractant and the attractant syrup of feeding, the inductive education honeybee is that pollination technique is crucial between the hybrid soybean seed farm.
China is a traditional soybean producing country, and soybean productive potential and soybean market demand are very big, and the present invention lures honeybee through the honeybee attractant; Be the hybrid soybean seeds pollination technique; Not only can solve the solid problem of pollination of hybrid soybean seeds, and, produce Tanaka's extensive use soybean and promote improving the hybrid soybean hybrid seed yield; Promote the large tracts of land raising the output of soybean production field, have crucial meaning.
Claims (3)
1. honeybee attractant, with honeycomb liquid 7.0～15g, imitative fragrance of a flower liquid 2.5 ~ 5.0g, syrup 5.0～10g that the white sugar of water and equivalent makes, cold water 70～85.5g, being mixed forms.
2. the preparation method of above-mentioned honeybee attractant, be made up of following process and step:
(1) honeybee nest spleen 1 ㎏ is got in the preparation of described honeycomb extract, is cut into fragment; Add 75% ethanol 1000ml, airtight immersion 72h is after the filtration of 100 order gauzes; It is for use to collect the broken honeycomb of filter residue, and it is filtrated and under 50 ℃-0.095Mpa vacuum, is concentrated into water content is 25% honeycomb liquid; Take the broken honeycomb after alcohol extract filters, add entry 1500ml, under 85～95 ℃ of stirrings, be concentrated to 800ml, stop heating, remove the beeswax that solidify on top after the cooling, filter with 100 order gauzes, waste is collected honeycomb liquid; The honeycomb liquid mixing of above-mentioned extracted twice is obtained honeycomb liquid;
(2) preparation of described imitative fragrance of a flower liquid; Get the smart 2.5～5.0g of pollen, evening primrose oil 2.5～5.0g, orange oil 2.5～5.0g, perilla herb oil 2.5～5.0g mixing are contained in the bottle, add 50%～75% ethanol 80～90 ml again, and its mixing is formulated;
(3) get honeycomb liquid 7.0～15g, imitative fragrance of a flower liquid 2.5 ~ 5.0g, syrup 5.0 ~ 10g that the white sugar of water and equivalent makes, cold water 70～85.5g, mixing make described honeybee attractant.
3. honeybee attractant as claimed in claim 1 is used for the method for hybrid soybean seeds field production of hybrid seeds honeybee pollination, is made up of following process and step:
(1) honeybee pollination hybrid soybean field is selected
According to regional climatic condition, select to be suitable for the field of soybeans they grow, by the normal field management hybrid soybean seeds of beginning sowing in good time, and implement to plant at a distance between ridge between male parent, the female parent;
(2) the pollination bee colony is selected
In the bee colony of normal breeding, select the pollination bee colony, the pollination bee colony will reach 8 frame honeybees, 7 crowd's gesture that sub-spleen is above; Wherein, it is several about 30% that foraging bee accounts for full crowd honeybee, and house bee accounts for about 70%; The healthy no swarming fever of bee colony, non-communicable disease;
(3) the pollination bee colony is transported soybeans
The pollination bee colony that the soybean blossoming initial stage will be selected is transported the hybrid soybean seeds seed farm, and per hectare is placed 3 ~ 6 groups in pollination bee colony;
(4) the honeybee attractant of spraying, feed
In between the hybrid soybean seeds seed farm of just having bloomed, every day or sprayed the attractant syrup of feeding preceding 1 hour implementing the honeybee attractant every other day; 1 ~ 5 ridge ground, per 1 ~ 3 day interval, the male parent in 1 ~ 5 ridge ground, hybridization of female parent soybean plant strain foliar spray mist honeybee attractant lure a large amount of honeybees to drop on the attractant syrup of searching for food on the hybrid soybean plants leaf gradually; To hybrid soybean strain stalk two side spray honeybee attractants, lure honeybee to climb up on the soybean strain stalk again, climb to hybrid soybean successively and take collection, thereby be the hybrid soybean pollination; Fine normal spraying honeybee attractant, overcast and rainy stopping to be sprayed; Recover spraying honeybee attractant after weather takes a turn for the better at once, be enough to the satisfied needed attractant of honeybee of luring to keep the field to have;
(5) the soybean florescence finishes, the honeybee attractant that stops to spray, and the bee colony that will pollinate is withdrawn hybrid soybean production of hybrid seeds ground.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210183837.5A CN102823628B (en) | 2011-12-27 | 2012-06-06 | Honey bee attractant and method for seed production and honeybee pollination of soybean hybrid seed in field |
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| CN 201110443277 CN102524049A (en) | 2011-12-27 | 2011-12-27 | Bee attractant and method for field seed production and bee pollination of soybean hybrids |
| CN201110443277.8 | 2011-12-27 | ||
| CN201210183837.5A CN102823628B (en) | 2011-12-27 | 2012-06-06 | Honey bee attractant and method for seed production and honeybee pollination of soybean hybrid seed in field |
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| CN102823628A true CN102823628A (en) | 2012-12-19 |
| CN102823628B CN102823628B (en) | 2014-02-19 |
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| CN 201110443277 Pending CN102524049A (en) | 2011-12-27 | 2011-12-27 | Bee attractant and method for field seed production and bee pollination of soybean hybrids |
| CN201210183837.5A Expired - Fee Related CN102823628B (en) | 2011-12-27 | 2012-06-06 | Honey bee attractant and method for seed production and honeybee pollination of soybean hybrid seed in field |
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| CN103988850A (en) * | 2013-05-20 | 2014-08-20 | 杨健 | Attractant capable of promoting cross-pollination and improving fruit set percentage of fruit tree |
| CN103270943A (en) * | 2013-05-20 | 2013-09-04 | 杨健 | Attractant capable of promoting cross pollination of fruit trees and improving fruit set percentage of fruit trees |
| CN105028179A (en) * | 2015-06-23 | 2015-11-11 | 潍坊友容实业有限公司 | Biological pollination method capable of achieving high pollination rate by adopting bee attractant |
| CN105028178A (en) * | 2015-06-23 | 2015-11-11 | 潍坊友容实业有限公司 | Method for carrying out biological pollination by adopting bees and application thereof to Chinese wolfberry planting in saline-alkali soil |
| CN106305790A (en) * | 2015-07-02 | 2017-01-11 | 甘肃省蜂业技术推广总站 | Method for preparing bee collecting agent |
| CN106035411B (en) * | 2016-06-15 | 2018-12-11 | 湖南省蔬菜研究所 | A kind of wall bee attractant for capsicum pollination |
| CN106035411A (en) * | 2016-06-15 | 2016-10-26 | 湖南省蔬菜研究所 | Osmia lure for chili pollination |
| WO2019021209A1 (en) | 2017-07-26 | 2019-01-31 | Consejo Nacional De Investigaciones Cientificas Y Tecnicas (Conicet) | Formulation for promoting targeted pollination of almond tree crops in honey bees |
| WO2019021210A1 (en) | 2017-07-26 | 2019-01-31 | Consejo Nacional De Investigaciones Cientificas Y Tecnicas (Conicet) | Formulation for promoting targeted pollination of pear tree crops in honey bees |
| CN107751113A (en) * | 2017-10-25 | 2018-03-06 | 桐梓县德毓蜂业发展有限公司 | It is a kind of to lure honeybee liquid and preparation method thereof for honeybee |
| CN107787835A (en) * | 2017-10-26 | 2018-03-13 | 田东县八闽农业综合开发有限公司 | A kind of method for luring honeybee to pollinate during the mango florescence |
| US11270189B2 (en) | 2019-10-28 | 2022-03-08 | International Business Machines Corporation | Cognitive decision platform for honey value chain |
| CN112535151A (en) * | 2020-12-08 | 2021-03-23 | 吉林省农业科学院 | Liquid composition for attracting bees, solid honey attractant and preparation method thereof |
| CN113367061B (en) * | 2021-05-29 | 2022-06-24 | 吉林省养蜂科学研究所（吉林省蜂产品质量管理监督站、吉林省蜜蜂遗传资源基因保护中心） | Method for improving pollination rate of soybeans |
| CN113367061A (en) * | 2021-05-29 | 2021-09-10 | 吉林省养蜂科学研究所（吉林省蜂产品质量管理监督站、吉林省蜜蜂遗传资源基因保护中心） | Method for improving pollination rate of soybeans |
| US20230056428A1 (en) * | 2021-08-11 | 2023-02-23 | The United States Of America, As Represented By The Secretary Of Agriculture | Compositions and methods for attracting fire ants |
| US11944090B2 (en) * | 2021-08-11 | 2024-04-02 | The United States Of America, As Represented By The Secretary Of Agriculture | Compositions and methods for attracting fire ants |
| WO2023031883A1 (en) | 2021-09-03 | 2023-03-09 | Beeflow Corporation | Formulation and composition which promote targeted pollination by bees towards blueberry crops and related methods |
| WO2023031882A1 (en) | 2021-09-03 | 2023-03-09 | Beeflow Corporation | Formulation and composition which promote targeted pollination by bees towards kiwifruit crops and related methods |
| CN115363033A (en) * | 2022-08-30 | 2022-11-22 | 吉林省养蜂科学研究所（吉林省蜂产品质量管理监督站、吉林省蜜蜂遗传资源基因保护中心） | Attractant for improving efficient pollination of facility watermelon and muskmelon bees, method and application |
| CN115363033B (en) * | 2022-08-30 | 2023-08-18 | 吉林省养蜂科学研究所（吉林省蜂产品质量管理监督站、吉林省蜜蜂遗传资源基因保护中心） | Attractant, method and application for improving efficient pollination of facility melon bees |
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| Publication number | Publication date |
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| CN102524049A (en) | 2012-07-04 |
| CN102823628B (en) | 2014-02-19 |
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