CN115363033B - Attractant, method and application for improving efficient pollination of facility melon bees - Google Patents

Attractant, method and application for improving efficient pollination of facility melon bees Download PDF

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Publication number
CN115363033B
CN115363033B CN202211052092.9A CN202211052092A CN115363033B CN 115363033 B CN115363033 B CN 115363033B CN 202211052092 A CN202211052092 A CN 202211052092A CN 115363033 B CN115363033 B CN 115363033B
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attractant
bees
pollination
melon
glucose
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CN115363033A (en
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闫德斌
李杰銮
徐凯
丁洪伟
常志光
杨春红
孙智禹
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Apiculture Science Institute Of Jilin Province (jilin Honeybee Product Quality Management Supervise Station Jilin Honeybee Heredity Resource Gene Protection Center)
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Apiculture Science Institute Of Jilin Province (jilin Honeybee Product Quality Management Supervise Station Jilin Honeybee Heredity Resource Gene Protection Center)
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/02Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
    • A01N43/04Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
    • A01N43/14Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings
    • A01N43/16Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings with oxygen as the ring hetero atom
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G22/00Cultivation of specific crops or plants not otherwise provided for
    • A01G22/05Fruit crops, e.g. strawberries, tomatoes or cucumbers
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G7/00Botany in general
    • A01G7/06Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01HNEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H1/00Processes for modifying genotypes ; Plants characterised by associated natural traits
    • A01H1/02Methods or apparatus for hybridisation; Artificial pollination ; Fertility
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01KANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
    • A01K67/033Rearing or breeding invertebrates; New breeds of invertebrates
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N35/00Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having two bonds to hetero atoms with at the most one bond to halogen, e.g. aldehyde radical
    • A01N35/02Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having two bonds to hetero atoms with at the most one bond to halogen, e.g. aldehyde radical containing aliphatically bound aldehyde or keto groups, or thio analogues thereof; Derivatives thereof, e.g. acetals
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01PBIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
    • A01P19/00Pest attractants
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01PBIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
    • A01P21/00Plant growth regulators
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K20/00Accessory food factors for animal feeding-stuffs
    • A23K20/10Organic substances
    • A23K20/116Heterocyclic compounds
    • A23K20/121Heterocyclic compounds containing oxygen or sulfur as hetero atom
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K20/00Accessory food factors for animal feeding-stuffs
    • A23K20/10Organic substances
    • A23K20/163Sugars; Polysaccharides

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  • Life Sciences & Earth Sciences (AREA)
  • Environmental Sciences (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Plant Pathology (AREA)
  • Botany (AREA)
  • Polymers & Plastics (AREA)
  • General Health & Medical Sciences (AREA)
  • Animal Husbandry (AREA)
  • Health & Medical Sciences (AREA)
  • Pest Control & Pesticides (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Ecology (AREA)
  • Forests & Forestry (AREA)
  • Dentistry (AREA)
  • Agronomy & Crop Science (AREA)
  • Food Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Developmental Biology & Embryology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

The invention discloses an attractant, a method and application for improving efficient pollination of facility melon bees, and belongs to the technical field of crop bee pollination. The invention discloses an attractant for improving efficient pollination of facility melon bees, which comprises the following components in parts by weight: 8-10 parts of tea polyphenol, 3-6 parts of maltol and 2-5 parts of glucose. Compared with the conventional attractant, the sugar content of the attractant is greatly reduced, the damage of diseases and insects caused by the high-sugar attractant can be avoided, meanwhile, the invention creatively carries out the sugar-reducing adaptability training before the pollination of bees, so that pollinated bees are gradually adapted to the smell of the attractant taking tea polyphenol as a main material and taking maltol and glucose as auxiliary materials, and during pollination, the bees which finish training can carry out more sensitive and efficient flower-visiting pollination, the fruit setting rate of the melon is improved, the abnormal fruit rate is reduced, and the yield of the melon is further increased.

Description

Attractant, method and application for improving efficient pollination of facility melon bees
Technical Field
The invention relates to the technical field of crop bee pollination, in particular to an attractant, a method and application for improving efficient pollination of facility melon bees.
Background
Along with the improvement of agricultural industrialization, the greenhouse film economy is continuously developed, the facility agriculture production modes are more and more, and the production area of the facility melon is also continuously enlarged. Because of the relatively closed production environment, growers often adopt hormone (such as cantaloupe) to assist in fruit setting and pollination or artificial pollination of the melons. However, the manual pollination technology for melon has the defects of large labor capacity, high employment cost, untimely pollination time and the like; the use of the melon-sitting pollination mode requires the mixing of hormones with different concentrations at different temperatures, and the existing preparation is inaccurate, so that stiff fruits or abnormal fruits are easy to generate to influence the production income, and meanwhile, under the increasing concerns of consumers on the nutrition quality and food safety of fruits, the artificial pollination and hormone assisted pollination technology cannot meet the development needs of the melon industry.
The bee pollination technology is an important technical guarantee for producing organic, green and pollution-free agricultural products, pollinates field and facility crops by using bees, is an important part of green food engineering construction, and can reduce the artificial pollination cost and improve the production income by adopting a bee pollination mode. However, in actual production, the bees are the phenomena of poor enthusiasm of nest-forming activity, low flower-visiting efficiency, non-ideal pollination effect and the like in the process of pollination of the facility melon, so that the bees attractant is generated.
In the prior researches, the common attractant formula is mostly flower juice, syrup or volatile compounds of various plants, and a large amount of sugar is mixed, and the prepared attractant is sprayed on the plants, so that various diseases of the plants are easily caused, and other pests are attracted. Although the prior art has developed low-sugar or sugar-free attractants for crops such as tomatoes, no report has been made so far on bee attractants for facility melons.
Disclosure of Invention
The invention aims to provide an attractant, a method and application for improving efficient pollination of facility melon bees, so as to solve the problems in the prior art.
In order to achieve the above object, the present invention provides the following solutions:
the invention provides an attractant for improving efficient pollination of facility melon bees, which comprises the following components in parts by weight: 8-10 parts of tea polyphenol, 3-6 parts of maltol and 2-5 parts of glucose.
Further, the attractant is prepared by mixing tea polyphenol, maltol and glucose according to a proportion and then dissolving the mixture in water.
Further, the feed liquid ratio of the mixture of tea polyphenol, maltol and glucose to water is 14-19g:500mL.
The invention also provides a method for improving the efficient pollination of the facility melon bees, which comprises the steps of firstly carrying out sugar reduction adaptability training on the bees, and then spraying the attractant for improving the efficient pollination of the facility melon bees on the melon flowers in the full bloom period.
Further, the glucose-reducing adaptation training comprises: the sugar-reducing attractant is fed every day 1-10 days before the bees pollinate.
Further, the glucose-reducing adaptation training comprises: 1-5d before pollination of bees, feeding the step-by-step sugar-reducing attractant every day, and feeding the attractant for improving efficient pollination of the facility melon bees in the 6 th-10 d.
Further, the formula of the progressive glucose-reducing attractant is a mixture of glucose, maltol and tea polyphenol in a mass ratio of 3-10:4-6:3-9.
Further, after spraying the attractant to the flowers of the melons in the full bloom stage, the method further comprises the step of placing bees which complete the adaptive training of sugar reduction into a greenhouse of the melons to be pollinated.
The invention also provides application of the attractant for improving efficient pollination of the facility melon bees in promoting production of the facility melon.
Further, the promotion of the production of the facility melon includes increasing the fruit setting rate and yield of the facility melon and decreasing the malformed fruit rate thereof.
The invention discloses the following technical effects:
compared with the conventional attractant, the bee attractant has the advantages that the sugar content is greatly reduced, the damage to diseases and insects caused by the high-sugar attractant can be avoided, meanwhile, the inventor team creatively performs sugar reduction adaptability training before pollination of bees, so that pollinated bees are gradually adapted to the smell of the attractant taking tea polyphenol as a main material and taking maltol and glucose as auxiliary materials, and during pollination, the bees which finish training can more sensitively and efficiently carry out flower-visiting pollination, the fruit setting rate of the melon is improved, the abnormal fruit setting rate is reduced, and the yield of the melon is further increased.
Detailed Description
Various exemplary embodiments of the invention will now be described in detail, which should not be considered as limiting the invention, but rather as more detailed descriptions of certain aspects, features and embodiments of the invention.
It is to be understood that the terminology used herein is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention. In addition, for numerical ranges in this disclosure, it is understood that each intermediate value between the upper and lower limits of the ranges is also specifically disclosed. Every smaller range between any stated value or stated range, and any other stated value or intermediate value within the stated range, is also encompassed within the invention. The upper and lower limits of these smaller ranges may independently be included or excluded in the range.
Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. Although only preferred methods and materials are described herein, any methods and materials similar or equivalent to those described herein can be used in the practice or testing of the present invention. All documents mentioned in this specification are incorporated by reference for the purpose of disclosing and describing the methods and/or materials associated with the documents. In case of conflict with any incorporated document, the present specification will control.
It will be apparent to those skilled in the art that various modifications and variations can be made in the specific embodiments of the invention described herein without departing from the scope or spirit of the invention. Other embodiments will be apparent to those skilled in the art from consideration of the specification of the present invention. The specification and examples of the present invention are exemplary only.
As used herein, the terms "comprising," "including," "having," "containing," and the like are intended to be inclusive and mean an inclusion, but not limited to.
The tea polyphenol, the maltol and the glucose components used in the invention are all commonly and commercially available, the bees used in the invention are italian bees, and the bee colony is prepared according to the conventional pollination standard.
Example 1
The invention prepares the attractant for improving the efficient pollination of the facility melon bees, and the components of the attractant comprise tea polyphenol, maltol and glucose; in order to solve the problems that the existing attractant contains a large amount of sugar, is easy to cause diseases when sprayed on plants and affects the growth of the plants, the inventor creatively carries out sugar-reducing adaptability training 10d before bee pollination, and the training is as follows:
day 1: the preparation method of the bee high sugar attractant comprises the following steps: weighing 10g, 6g and 3g of glucose, maltol and tea polyphenol respectively, mixing and dissolving in 500mL of purified water to obtain a high-sugar attractant;
day 2: feeding bees with the sugar-reducing attractant 1, wherein the formula comprises 8g, 5g and 4g of glucose, maltol and tea polyphenol respectively, and the preparation method is the same as that described above;
day 3: feeding the bee sugar-reducing attractant 2, wherein the formula comprises 7g, 6g and 5g of glucose, maltol and tea polyphenol respectively;
day 4: feeding a bee sugar reducing attractant 3, wherein the formula comprises 5g, 5g and 7g of glucose, maltol and tea polyphenol respectively;
day 5: feeding a bee sugar reducing attractant 4, wherein the formula comprises 3g, 4g and 9g of glucose, maltol and tea polyphenol respectively;
day 6: feeding a bee sugar-reducing attractant 5 (bee attractant) which comprises 2 g, 4g and 8g of glucose, maltol and tea polyphenol respectively;
the bee sugar reducing attractant 5 is continuously fed on days 7-10.
And 11-20d, spraying the bee attractant on the flowers of the melons in the full bloom stage, and inducing the bee to carry out the melissa pollination after the adaptive training of sugar reduction.
Example 2
The difference from example 1 is that the bee attractant formulation is 3, 6 and 10g of glucose, maltol and tea polyphenols, respectively.
Example 3
The difference from example 1 is that the bee attractant formulation is 5, 3 and 9g of glucose, maltol and tea polyphenols, respectively.
Comparative example 1
The difference from example 1 is that pollinated bees were not trained for glucose-reducing adaptation.
Comparative example 2
The difference from example 1 is that the bee attractant is a common commercial attractant, and the main components are plant fragrance and white sugar.
Comparative example 3
The difference from example 1 is that no bee attractant was sprayed.
Comparative example 4
The difference from example 1 is that the bee attractant omits the maltol component.
Comparative example 5
The difference from example 1 is that the bee attractant omits the tea polyphenol component.
Effect verification
1. 10 days before using bees to pollinate greenhouse melon, selecting 9 groups of italian bee pollinate bees with uniform swarm size and health without diseases, wherein each group of pollinate bees contains 3 honeycombs, the honey powder is sufficiently reserved, the worker bees are 2-spleen, and the queen bee is healthy and can lay eggs. Each group of bees is correspondingly pollinated after being treated according to the following schemes.
The treatment group which is not subjected to sugar reduction adaptive training and does not spray the bee attractant is taken as a blank control group, 9 greenhouses with consistent management mode and melon growth vigor are selected, bees and melons in the full bloom stage are treated according to the technical schemes of examples 1-3 and comparative examples 1-5 and the blank control group, the bee attractant is sprayed on flowers at the early 9:00 (except the comparative example 3 and the blank control group), the number of bees visiting flowers in each group is recorded after one hour, each experiment is recorded for 20min, and the result is as follows:
TABLE 1
2. The melon pollinated by bees is managed and cultured according to a conventional greenhouse, and after the melon pollinated by bees is mature, the fruit setting rate, the malformed fruit rate and the yield of each group are counted, and the result is as follows:
TABLE 2
Group of Fruit setting rate (%) Malformed fruit rate (%) Mu yield (kg/mu)
Example 1 93.8 1.3 3820
Example 2 94.1 1.0 3910
Example 3 93.7 1.1 3870
Comparative example 1 85.3 6.2 2990
Comparative example 2 79.4 7.3 2880
Comparative example 3 67.3 9.6 2560
Comparative example 4 78.2 8.4 2830
Comparative example 5 74.5 8.9 2780
Blank control group 64.5 13.6 2040
By combining the experimental data in tables 1 and 2, the bees trained by the sugar reduction adaptability of the invention have the advantages that compared with the common high-sugar attractant, the low-sugar bee attractant is more preferred, the bee flower visiting efficiency is improved, the fruit setting rate and the yield of the melon are further increased, and the abnormal fruit rate is reduced. In addition, the weight proportion of the creative preparation components of the invention is 8-10:3-6:2-5, after 10d sugar-reducing adaptability training, bees gradually adapt to the attractant odor which takes tea polyphenol as a main material and takes malt phenol and glucose as auxiliary materials, and the reason for improving the bee pollination efficiency by the synergistic cooperation of the components of the low-sugar attractant is as follows:
firstly, the tea polyphenol has strong function of eliminating harmful free radicals, can inhibit harmful substances, and can be sprayed on the surfaces of flowers, so that the flowers are brighter and more vivid in color and more attractive to bees; secondly, the tea polyphenol can improve the memory and the odor sensitivity of bees after training, so that the bees can more efficiently visit flowers and pollinate; thirdly, the maltol solution has strawberry-like aromatic taste, is matched with tea polyphenol and glucose in a synergistic way, has delicate flavor and sweet and refreshing mouthfeel, and guides bees to prefer to collect.
The above embodiments are only illustrative of the preferred embodiments of the present invention and are not intended to limit the scope of the present invention, and various modifications and improvements made by those skilled in the art to the technical solutions of the present invention should fall within the protection scope defined by the claims of the present invention without departing from the design spirit of the present invention.

Claims (7)

1. The attractant for improving bee pollination of the protected muskmelon is characterized by comprising the following components in parts by weight: 8-10 parts of tea polyphenol, 3-6 parts of maltol and 2-5 parts of glucose.
2. An attractant for improving pollination of protected melon bees according to claim 1, wherein the attractant is prepared by mixing tea polyphenols, maltol and glucose in a ratio and dissolving in water.
3. An attractant for increasing pollination of protected melon bees according to claim 2, wherein the feed ratio of the mixture of tea polyphenols, maltol and glucose to water is 14-19g:500mL.
4. A method for improving pollination of bees of protected melons, which is characterized in that bees are subjected to adaptive training for reducing sugar, and then the attractant for improving pollination of bees of protected melons, which is in full bloom stage, is sprayed to the flowers of the melons, which are in full bloom stage, according to any one of claims 1-3;
the glucose-reducing fitness training comprises: pollination is carried out on bees for 1-5d, step-by-step sugar-reducing attractants are fed every day, and the attractants for improving the pollination of the facility melon bees are fed for 6-10 d;
the gradual sugar reduction attractant is a mixture of glucose, maltol and tea polyphenol in a mass ratio of 3-10:4-6:3-9.
5. The method of claim 4, further comprising placing bees that have completed the adaptive training for sugar reduction in the greenhouse of the melon to be pollinated after spraying the attractant to the flowers of the melon in the full bloom stage.
6. Use of an attractant according to any one of claims 1 to 3 to enhance bee pollination of protected melon in promoting production of protected melon.
7. The use according to claim 6, wherein said promoting production of facility melon comprises increasing fruit set rate and yield of said facility melon and decreasing malformed fruit rate thereof.
CN202211052092.9A 2022-08-30 2022-08-30 Attractant, method and application for improving efficient pollination of facility melon bees Active CN115363033B (en)

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Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH11130612A (en) * 1997-10-24 1999-05-18 Nippon Flour Mills Co Ltd Attractant and bait for insect
CN102823628A (en) * 2011-12-27 2012-12-19 吉林省养蜂科学研究所 Honey bee attractant and method for seed production and honeybee pollination of soybean hybrid seed in field
EP2735229A1 (en) * 2012-11-27 2014-05-28 SC Sunshine Business Serv SRL Product for treatment of bee families and process for its application
EP2949220A1 (en) * 2014-05-27 2015-12-02 SC Teso Spec SRL Nutraceutic supplement for bees families and process for its administration

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH11130612A (en) * 1997-10-24 1999-05-18 Nippon Flour Mills Co Ltd Attractant and bait for insect
CN102823628A (en) * 2011-12-27 2012-12-19 吉林省养蜂科学研究所 Honey bee attractant and method for seed production and honeybee pollination of soybean hybrid seed in field
EP2735229A1 (en) * 2012-11-27 2014-05-28 SC Sunshine Business Serv SRL Product for treatment of bee families and process for its application
EP2949220A1 (en) * 2014-05-27 2015-12-02 SC Teso Spec SRL Nutraceutic supplement for bees families and process for its administration

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