CN115363033A - Attractant for improving efficient pollination of facility watermelon and muskmelon bees, method and application - Google Patents

Attractant for improving efficient pollination of facility watermelon and muskmelon bees, method and application Download PDF

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CN115363033A
CN115363033A CN202211052092.9A CN202211052092A CN115363033A CN 115363033 A CN115363033 A CN 115363033A CN 202211052092 A CN202211052092 A CN 202211052092A CN 115363033 A CN115363033 A CN 115363033A
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attractant
pollination
bees
watermelon
facility
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CN115363033B (en
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闫德斌
李杰銮
徐凯
丁洪伟
常志光
杨春红
孙智禹
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Apiculture Science Institute Of Jilin Province (jilin Honeybee Product Quality Management Supervise Station Jilin Honeybee Heredity Resource Gene Protection Center)
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Apiculture Science Institute Of Jilin Province (jilin Honeybee Product Quality Management Supervise Station Jilin Honeybee Heredity Resource Gene Protection Center)
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/02Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
    • A01N43/04Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
    • A01N43/14Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings
    • A01N43/16Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings with oxygen as the ring hetero atom
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G22/00Cultivation of specific crops or plants not otherwise provided for
    • A01G22/05Fruit crops, e.g. strawberries, tomatoes or cucumbers
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G7/00Botany in general
    • A01G7/06Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01HNEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H1/00Processes for modifying genotypes ; Plants characterised by associated natural traits
    • A01H1/02Methods or apparatus for hybridisation; Artificial pollination ; Fertility
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01KANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
    • A01K67/033Rearing or breeding invertebrates; New breeds of invertebrates
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N35/00Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having two bonds to hetero atoms with at the most one bond to halogen, e.g. aldehyde radical
    • A01N35/02Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having two bonds to hetero atoms with at the most one bond to halogen, e.g. aldehyde radical containing aliphatically bound aldehyde or keto groups, or thio analogues thereof; Derivatives thereof, e.g. acetals
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01PBIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
    • A01P19/00Pest attractants
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01PBIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
    • A01P21/00Plant growth regulators
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K20/00Accessory food factors for animal feeding-stuffs
    • A23K20/10Organic substances
    • A23K20/116Heterocyclic compounds
    • A23K20/121Heterocyclic compounds containing oxygen or sulfur as hetero atom
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K20/00Accessory food factors for animal feeding-stuffs
    • A23K20/10Organic substances
    • A23K20/163Sugars; Polysaccharides

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  • Life Sciences & Earth Sciences (AREA)
  • Environmental Sciences (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Plant Pathology (AREA)
  • Botany (AREA)
  • Polymers & Plastics (AREA)
  • General Health & Medical Sciences (AREA)
  • Animal Husbandry (AREA)
  • Health & Medical Sciences (AREA)
  • Pest Control & Pesticides (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Ecology (AREA)
  • Forests & Forestry (AREA)
  • Dentistry (AREA)
  • Agronomy & Crop Science (AREA)
  • Food Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Developmental Biology & Embryology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

The invention discloses an attractant for improving efficient pollination of facility watermelon and muskmelon bees, a method and application, and belongs to the technical field of bee pollination of crops. The invention discloses an attractant for improving efficient pollination of facility watermelon and muskmelon bees, which comprises the following components in parts by weight: 8-10 parts of tea polyphenol, 3-6 parts of maltol and 2-5 parts of glucose. Compared with the conventional attractant, the sugar content of the attractant is greatly reduced, the disease and pest damage caused by the high-sugar attractant can be avoided, meanwhile, sugar-reducing adaptive training is creatively carried out before bee pollination, pollinated bees gradually adapt to the odor of the attractant taking tea polyphenol as a main material and maltol and glucose as auxiliary materials, and during pollination, the trained bees can more sensitively and efficiently visit flowers and pollinate, the fruit setting rate of the watermelon melon is improved, the malformed fruit rate is reduced, and the yield of the facility watermelon melon is increased.

Description

Attractant for improving efficient pollination of facility watermelon and muskmelon bees, method and application
Technical Field
The invention relates to the technical field of bee pollination of crops, in particular to an attractant and a method for improving efficient pollination of facility watermelon and muskmelon bees and application.
Background
With the improvement of agricultural industrialization, greenhouse film economy is continuously developed, facility agricultural production modes are more and more, and the production area of facility watermelon is also continuously enlarged. Because of the relatively closed production environment, growers often adopt hormones (such as dicentrine) to assist fruit setting and pollination or artificially pollinate melons. However, the manual pollination technology for melons has the defects of large labor capacity, high employee cost, untimely pollination time and the like; the method for pollination by using zucchini requires matching of different concentrations of hormones at different temperatures, and existing pollination is used, if the method is not accurately mastered, dead fruits or malformed fruits are easily generated, so that the production yield is influenced, and meanwhile, under the increasing concern of consumers on the nutritional quality and food safety of fruits, the artificial pollination and hormone-assisted pollination technologies cannot meet the requirement of development of the west melon industry.
The bee pollination technology is an important technical guarantee for the production of organic, green and pollution-free agricultural products, bees are utilized for pollination of field and facility crops, and the bee pollination technology is an important part of green food engineering construction, and meanwhile, the bee pollination mode is adopted, so that the artificial pollination cost can be reduced, and the production income can be improved. However, in the actual production, in the process of pollination of the watermelon by bees as a facility, the phenomena of poor activity enthusiasm of nest outlet, low flower visiting efficiency, unsatisfactory pollination effect and the like exist, so that the bee attractant is generated at the right moment.
In the existing research, most of common attractant formulas are flower juice, syrup or volatile compounds of various plants, a large amount of sugar is mixed, and the prepared attractant is sprayed on the plants, so that the plants are easy to suffer from various diseases, and other pests can be attracted. Although the prior art relates to the development of a low-sugar or no-sugar attractant for crops such as tomatoes, and the like, no report on a honeybee attractant for facility melons is found so far.
Disclosure of Invention
The invention aims to provide an attractant for improving the efficient pollination of facility watermelon and muskmelon bees, a method and application, and aims to solve the problems in the prior art.
In order to achieve the purpose, the invention provides the following scheme:
the invention provides an attractant for improving efficient pollination of facility watermelon and muskmelon bees, which comprises the following components in parts by weight: 8-10 parts of tea polyphenol, 3-6 parts of maltol and 2-5 parts of glucose.
Further, the attractant is prepared by mixing tea polyphenol, maltol and glucose according to a ratio and then dissolving the mixture in water.
Further, the material-liquid ratio of the mixture of the tea polyphenol, the maltol and the glucose to the water is 14-19g:500mL.
The invention also provides a method for improving the efficient pollination of the facility watermelon and muskmelon, which comprises the steps of firstly carrying out sugar-reducing adaptive training on bees and then spraying the attractant for improving the efficient pollination of the facility watermelon and muskmelon on flowers in the full-bloom stage.
Further, the sugar-reducing adaptive training comprises: feeding sugar-reducing attractant 1-10 days before pollination.
Further, the sugar-reducing adaptive training comprises: and feeding an attractant for gradually reducing sugar every day 1-5 days before pollination of the bees, and feeding the attractant for improving the high-efficiency pollination of the facility watermelon and melon at 6-10 days.
Further, the formula of the gradual sugar-reducing attractant is a mixture consisting of glucose, maltol and tea polyphenol in a mass ratio of 3-10.
Further, after the attractant is sprayed to the watermelon and melon flowers in the full-bloom stage, the method also comprises the step of placing the bees which finish the sugar-reducing adaptability training into the watermelon and melon greenhouse to be pollinated.
The invention also provides application of the attractant for improving the efficient pollination of the facility watermelon honey bees in promoting the facility watermelon production.
Further, the facilitating the facility watermelon production comprises increasing the fruit set rate and yield of the facility watermelon, and decreasing the malformed fruit rate.
The invention discloses the following technical effects:
compared with the conventional attractant, the sugar content of the bee attractant is greatly reduced, the pest and disease damage caused by the high-sugar attractant can be avoided, meanwhile, an inventor group creatively carries out sugar-reducing adaptive training before bee pollination, so that pollinated bees gradually adapt to the odor of the attractant with tea polyphenol as a main material and maltol and glucose as auxiliary materials, and the trained bees can more sensitively and efficiently visit flowers and pollinate during pollination, the fruit setting rate of the watermelon melon is improved, the malformed fruit rate is reduced, and the yield of the facility watermelon melon is increased.
Detailed Description
Reference will now be made in detail to various exemplary embodiments of the invention, the detailed description should not be construed as limiting the invention but rather as a more detailed description of certain aspects, features and embodiments of the invention.
It is to be understood that the terminology used herein is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention. Further, for numerical ranges in this disclosure, it is understood that each intervening value, between the upper and lower limit of that range, is also specifically disclosed. Every smaller range between any stated value or intervening value in a stated range and any other stated or intervening value in a stated range is encompassed within the invention. The upper and lower limits of these smaller ranges may independently be included or excluded in the range.
Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. Although only preferred methods and materials are described herein, any methods and materials similar or equivalent to those described herein can be used in the practice or testing of the present invention. All documents mentioned in this specification are incorporated by reference herein for the purpose of disclosing and describing the methods and/or materials associated with the documents. In case of conflict with any incorporated document, the present specification will control.
It will be apparent to those skilled in the art that various modifications and variations can be made in the specific embodiments of the present disclosure without departing from the scope or spirit of the disclosure. Other embodiments will be apparent to those skilled in the art from consideration of the specification. The description and examples are intended to be illustrative only.
As used herein, the terms "comprising," "including," "having," "containing," and the like are open-ended terms that mean including but not limited to.
The tea polyphenol, maltol and glucose components used in the invention are all obtained from common markets, the used bees are Italian bees, and bee colonies are prepared according to the conventional pollination standard.
Example 1
The attractant for improving the efficient pollination of the facility watermelon melon honeybees is prepared by the invention, and the components of the attractant comprise tea polyphenol, maltol and glucose; in order to overcome the problems that the existing attractant contains a large amount of sugar, is easy to cause diseases when being sprayed on plants and influences the growth of the plants, an inventor team creatively carries out sugar-reducing adaptive training 10 days before bee pollination, and the training is as follows:
day 1: the high-sugar attractant for feeding bees comprises the following preparation method: weighing 10g, 6g and 3g of glucose, maltol and tea polyphenol respectively, mixing and dissolving in 500mL of purified water to obtain a high-sugar attractant;
day 2: feeding bee sugar-reducing attractant 1, wherein the formula comprises glucose, maltol and tea polyphenols of 8g, 5g and 4g respectively, and the preparation method is the same as above;
day 3: feeding a bee sugar-reducing attractant 2, wherein the formula comprises glucose, maltol and tea polyphenol respectively 7g, 6g and 5g;
day 4: feeding bee sugar-reducing attractant 3, wherein the formula comprises 5g, 5g and 7g of glucose, maltol and tea polyphenol respectively;
day 5: feeding bee sugar-reducing attractant 4, wherein the formula comprises 3g, 4g and 9g of glucose, maltol and tea polyphenol respectively;
day 6: feeding bee sugar-reducing attractant 5 (bee attractant) with the formula of 2 g, 4g and 8g of glucose, maltol and tea polyphenol respectively;
continuously feeding the honey bees with the sugar-reducing attractant 5 on the 7 th to 10 th days.
And (11) spraying the bee attractant on the flower of the watermelon and melon in the full-bloom stage to induce the bees trained in sugar-reducing adaptability to visit flowers and pollinate.
Example 2
The difference from example 1 is that the bee attractant formulations were 3, 6 and 10g each of glucose, maltol and tea polyphenols.
Example 3
The difference from example 1 is that the bee attractant formulations were 5, 3 and 9g each of glucose, maltol and tea polyphenols.
Comparative example 1
The difference from example 1 is that the pollinated bees have not been acclimatized for sugar reduction.
Comparative example 2
The difference from the example 1 is that the bee attractant is a common commercial attractant, and the main components are plant fragrance and white sugar.
Comparative example 3
The difference from example 1 is that no bee attractant was sprayed.
Comparative example 4
The difference from example 1 is that the bee attractant omits the maltol component.
Comparative example 5
The difference from example 1 is that the bee attractant omits the tea polyphenol component.
Effect verification
1. 10 days before bees are used for pollinating the greenhouse watermelon and melon, 9 groups of healthy and disease-free Italian bee pollination bee colonies with the same size are selected, each group of pollination bee contains 3 honeycombs, the honey powder is sufficient in reserve, 2 honeycombs of worker bees, and queen bees are healthy and can lay eggs. Each swarm of bees are treated according to the following formula and then pollinated correspondingly.
Selecting a treatment group which is not subjected to sugar-reducing adaptive training and is not sprayed with bee attractant as a blank control group, selecting 9 greenhouses with consistent management modes and watermelon and melon growth vigor, treating the bees and the watermelon and melon in the full-bloom stage according to the technical schemes of examples 1-3 and comparative examples 1-5 and the blank control group, spraying the bee attractant on the flowers at the early stage of 9 (except for the comparative example 3 and the blank control group), starting to record the number of bees which visit the flowers of each group after one hour, recording 20min in each experiment, and performing 5 times in total, wherein the results are as follows:
TABLE 1
Figure BDA0003824052830000071
2. According to the conventional greenhouse management, the watermelon and melon after bee pollination are cultured, after the watermelon and melon are mature, the fruit setting rate, the malformed fruit rate and the yield of each group are counted, and the results are as follows:
TABLE 2
Group of Percentage of fruit set (%) Rate of malformed fruits (%) Yield per mu (kg/mu)
Example 1 93.8 1.3 3820
Example 2 94.1 1.0 3910
Example 3 93.7 1.1 3870
Comparative example 1 85.3 6.2 2990
Comparative example 2 79.4 7.3 2880
Comparative example 3 67.3 9.6 2560
Comparative example 4 78.2 8.4 2830
Comparative example 5 74.5 8.9 2780
Blank control group 64.5 13.6 2040
By combining the experimental data in tables 1 and 2, the bees subjected to sugar-reducing adaptive training of the invention are more preferable to low-sugar bee attractants than common high-sugar attractants, so that the bee flower-visiting efficiency is improved, the fruit setting rate and the yield of the watermelon and the melon are increased, and the malformed fruit rate is reduced. In addition, the inventive preparation components have the weight ratio of 8-10:3-6:2-5, after 10d of sugar-reducing adaptive training, the bees gradually adapt to the odor of the attractant taking tea polyphenol as a main material and maltol and glucose as auxiliary materials, and the low-sugar attractant has the following reasons for improving the bee pollination efficiency by the cooperative matching of the components:
firstly, the tea polyphenol has strong function of eliminating harmful free radicals, can inhibit harmful substances, and can ensure that the color of the flower is brighter and more attractive to bees when being sprayed on the surface of the flower; secondly, the tea polyphenol can improve the memory and the smell sensitivity of the trained bees, so that the bees can more efficiently visit flowers and pollinate; thirdly, the maltol solution has strawberry-like aromatic taste, is cooperatively compatible with the tea polyphenol and the glucose, has faint scent flavor and sweet taste, and guides bees to prefer to collect.
The above-described embodiments are only intended to illustrate the preferred embodiments of the present invention, and not to limit the scope of the present invention, and various modifications and improvements made to the technical solution of the present invention by those skilled in the art without departing from the spirit of the present invention should fall within the protection scope defined by the claims of the present invention.

Claims (10)

1. An attractant for improving efficient pollination of facility watermelon and muskmelon bees is characterized by comprising the following components in parts by weight: 8-10 parts of tea polyphenol, 3-6 parts of maltol and 2-5 parts of glucose.
2. The attractant for improving the efficient pollination of facility melon bees as claimed in claim 1, wherein the attractant is prepared by mixing tea polyphenol, maltol and glucose according to a ratio and then dissolving the mixture in water.
3. The attractant for improving the efficient pollination of facility watermelon melon bees according to claim 2, wherein the feed-liquid ratio of the mixture of tea polyphenol, maltol and glucose to water is 14-19g:500mL.
4. A method for improving efficient pollination of facility watermelon and melon is characterized in that sugar-reducing adaptive training is carried out on bees, and then the attractant for improving efficient pollination of facility watermelon and melon as claimed in any one of claims 1-3 is sprayed on watermelon and melon flowers in full bloom stage.
5. The method of claim 4, wherein the sugar-reduction adaptive training comprises: feeding sugar-reducing attractant 1-10 days before pollination.
6. The method of claim 5, wherein the sugar-reduction adaptive training comprises: and feeding an attractant for gradually reducing sugar every day 1-5 days before pollination of the bees, and feeding the attractant for improving the high-efficiency pollination of the facility watermelon and melon at 6-10 days.
7. The method as claimed in claim 6, wherein the de-sugared attractant formula is a mixture of glucose, maltol and tea polyphenols in a mass ratio of 3-10.
8. The method as claimed in claim 4, wherein after spraying the attractant to the flowers of the watermelon in full bloom stage, the method further comprises placing the bees with the sugar-reducing adaptability training in the greenhouse of the watermelon to be pollinated.
9. Use of the attractant of any one of claims 1 to 3 for improving the efficient pollination of a facility melon honeybee to facilitate facility melon production.
10. The use of claim 9, wherein said facilitating facility watermelon production comprises increasing fruit set and yield and decreasing malformed fruit rate of said facility watermelon.
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Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH11130612A (en) * 1997-10-24 1999-05-18 Nippon Flour Mills Co Ltd Attractant and bait for insect
CN102823628A (en) * 2011-12-27 2012-12-19 吉林省养蜂科学研究所 Honey bee attractant and method for seed production and honeybee pollination of soybean hybrid seed in field
EP2735229A1 (en) * 2012-11-27 2014-05-28 SC Sunshine Business Serv SRL Product for treatment of bee families and process for its application
EP2949220A1 (en) * 2014-05-27 2015-12-02 SC Teso Spec SRL Nutraceutic supplement for bees families and process for its administration

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH11130612A (en) * 1997-10-24 1999-05-18 Nippon Flour Mills Co Ltd Attractant and bait for insect
CN102823628A (en) * 2011-12-27 2012-12-19 吉林省养蜂科学研究所 Honey bee attractant and method for seed production and honeybee pollination of soybean hybrid seed in field
EP2735229A1 (en) * 2012-11-27 2014-05-28 SC Sunshine Business Serv SRL Product for treatment of bee families and process for its application
EP2949220A1 (en) * 2014-05-27 2015-12-02 SC Teso Spec SRL Nutraceutic supplement for bees families and process for its administration

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