CN101116419A - Centipede grass cross-breeding method - Google Patents
Centipede grass cross-breeding method Download PDFInfo
- Publication number
- CN101116419A CN101116419A CNA2007100232422A CN200710023242A CN101116419A CN 101116419 A CN101116419 A CN 101116419A CN A2007100232422 A CNA2007100232422 A CN A2007100232422A CN 200710023242 A CN200710023242 A CN 200710023242A CN 101116419 A CN101116419 A CN 101116419A
- Authority
- CN
- China
- Prior art keywords
- centipede grass
- breeding method
- fringe
- pollination
- cross
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
The invention discloses a cross breeding method for eremochloa ophiuroides, comprising the choice of spica, manual detasseling, artificial supplementary pollination, the management after pollination and the seed harvest. The invention is characterized in that a technical cross breeding method for eremochloa ophiuroides is presented on the basis of the flowering habit and the property of certain self-fertilization fruitfulness rate. The red shank line of the eremochloa ophiuroides taken as female parent and the green shank line of the eremochloa ophiuroides taken as male parent are hybridized by utilizing the method to obtain the filial generation with green shank. The cross breeding method has simple manipulation and the filial generation with real, reliable and clear background, thereby providing an effective cross breeding method for the breeding of fine variety of eremochloa ophiuroides with various usages and providing an effective method for the usage of and the improvement of the abundant eremochloa ophiuroides in China.
Description
Technical field
The invention belongs to the technical field of plant hybridization breeding.
Background technology
Centipede grass Eremochloa ophiuroides (Munro.) Hack. originates from China middle part and south,, impoverishment tolerant strong with low, the easy planting of maintenance level, acid resistance and damage by disease and insect are few and celebrated, be external Chinese best warm season turf of generally acknowledging, so be called " Chinese turfgrass " (Chinese lawn grass) again.Though forefathers carried out research to the aspects such as outside proterties, ripening rate and flowering habit of centipede grass, all yet there are no the report about centipede grass cross-breeding method at home and abroad.Used kind all is to cultivate by the screening to wild resource and produce upward at present, has many drawbacks in screening process, also the merit of domestic affluent resources effectively can't be integrated, and can not reach the purpose of orderly improvement.For this reason, the present invention is on the basis that the centipede grass flowering habit is further observed, adopt artificial emasculation, manually got the mode of powder and artificial pollination, carry out the hybridization work of centipede grass, under the situation of understanding this characteristic of father and mother, characteristic to parents is carried out autotelic integration, obtains real centipede grass hybrid seed, for the utilization improvement of the centipede grass germ plasm resource of China's abundant provides a new approach.
The content of invention
The objective of the invention is to invent out the method that is suitable for centipede grass cross-breeding, for the centipede grass germ plasm resource that makes full use of China's abundant provides a simple method by research to the centipede grass hybridization conditions.
Centipede grass cross-breeding method of the present invention, it is characterized in that selecting the existing a small amount of little colored fringe that the flowers are in blossom puts in centipede grass top, immature having spent for a short time of open Xiao Hua in the Hua Sui top of choosing and fringe bottom removed, under magnifying glass, extract the stamen that the fringe middle part remains Xiao Hua, remove male flower and the male flower of Xiao Hua on the flower fringe fully with tweezers.Castrate the back and carry out bagging with ox-hide pouch made of paper, adopt the mode of manually getting powder and artificial pollination, carry out the hybridization work of centipede grass, under the situation of understanding this characteristic of father and mother, characteristic to parents is carried out autotelic integration, obtains real centipede grass hybrid seed.Utilize the method, we are maternal with the centipede grass red shank and green stem is that male parent is hybridized, and have obtained the stem look and have been green filial generation.
The technical solution adopted in the present invention is specific as follows:
1, the observation of centipede grass flowering habit
The centipede grass florescence is season in full summer, and outside air temperature is higher, and when blooming, Xiao Hua is open from top to down, and spend first of tip of the spike, little the flowers are in blossom is put into last little the flowers are in blossom puts successively lasting about 3～5 day time.Each small ear of centipede grass is made of a hermaphrodite flower and a male flower, and hermaphrodite flower was open before this when blooming, and gynoecium and stamen expose simultaneously, and at this moment male flower is not open.And when the hermaphrodite flower of Hua Sui bottom was open, the male flower on top began open, loose powder.Generally speaking, the morning, 7:30～8:30 was the easiest period of getting powder, was the window of opportunity of pollinating between the 8:00～9:00 in the morning.
2, the enforcement of crossbreeding
(1) spend ear selection to select
The plant of selecting robust growth, no damage by disease and insect is as female parent, flowering habit according to observed centipede grass, when centipede grass flower fringe is castrated, should select the existing a small amount of little tassel that the flowers are in blossom puts in top as the eunuchs fringe, because so little flower development in colored fringe middle part is near ripe, castrate the back and can pollinate next day, help improving hybridization efficiency.
(2) artificial emasculation
Because centipede grass has certain self-fruitful rate, therefore the stamen and the male Xiao Hua that must remove among the Xiao Hua earlier hybridized again.The time of centipede grass artificial emasculation is typically chosen in carried out up to 6 o'clock afternoon 3, Xiao Hua and the unopened Xiao Hua in bottom that the tassel top of choosing is open extract in advance, under magnifying glass, utilize tweezers to extract all male flower on the stamen of open Xiao Hua and the fringe of fringe middle part, to give special heed to gynoecium and the column cap of the Xiao Hua that to damage in the castration process, Xiao Hua is coetonium also will to be noted being kept perfectly, in order to avoid it is solid to cause the later stage normally not pollinate.Keep and coetoniumly not only can effectively preserve moisture for column cap, and the natural threshing can prevent most of seed maturity the time.After castration was finished, the thin kraft paper bag of utilization carried out the bagging isolation to the flower fringe and preserves moisture.
(3) manually get powder and pollination
Generally speaking, the morning, 7:30～8:30 pollen was maximum, was the easiest period of getting powder.Among the present invention, 7:30～8:30 in the morning, the male parent flower fringe bending that Xiao Hua is just open is placed in the little glass, knock the fringe body gently with pincet, allow paternal pollen be scattered in the little glass, as long as grasp the time that pollen is collected, the pollen amount of centipede grass can satisfy the hybridization pollination needs.
Being the period that centipede grass blooms and comparatively concentrates between the 8:00～9:00 in the morning, also is the window of opportunity of pollinating simultaneously.When pollinating among the present invention; with the pollen that has just taken off from male parent; dipping in brush with little writing brush stretches out on the coetonium column cap at gynoecium after the castration; because the top-down characteristics of centipede grass flower tassel blossom; therefore each flower Xiao Hua that fringe stays can not need pollination continuously just can guarantee that Hua Sui went up Xiao Hua and all awarded powder in 2～3 days open together on the same day yet.After finishing artificial supplementary pollination, will spend fringe to use the kraft paper bag bagging until seed maturity.
(4) management after the hybridization
After the hybridization, also have the Xiao Hua that does not extract to grow, checked once in per three days, find newly to grow Xiao Hua, extract immediately, check altogether five times for preventing the Hua Sui bottom.During hybridizing, for the growth of flower fringe, pollination, maturation, checked once every five days, often water, keep humidity.
(5) results management
Owing to castrate the coetonium injury of Xiao Hua in earlier stage, can not effectively wrap seed, therefore can to cause the shattering of centipede grass hybrid seed, but to obtain the quality of seed in order guaranteeing, therefore the seed of can not gathering was too early again gathered in the crops preceding 10～15 days at seed,
To seal the end opening of kraft paper bag with adhesive tape, in order to avoid seed comes off and can not receive seed.After waiting to spend the fringe cob all withered and yellow whole colored fringe is cut the results seed.
The effect that the present invention compares with other technologies:
Because at present centipede grass hybridization aspect yet there are no relevant research report, so this research is first the crossbreeding technology of centipede grass to be explored, and invented a kind of new technology that is suitable for centipede grass cross-breeding.
Specific embodiment:
Embodiment: 1
1, be that female parent, green stem are that male parent is carried out hybrid experiment with the centipede grass red shank.
(1) spend ear selection to select
On centipede grass red shank based material, select the existing a small amount of little tassel that the flowers are in blossom puts in top as the eunuchs fringe.
(2) artificial emasculation
Carried out in 3 o'clock in the afternoon, Xiao Hua and the unopened Xiao Hua in bottom that the tassel top of choosing is open extract in advance, only stay 10 small ears at the fringe middle part, under magnifying glass, utilize tweezers to extract all male flower on the stamen of open Xiao Hua and the fringe, to give special heed to gynoecium and the column cap of the Xiao Hua that to damage in the castration process, Xiao Hua is coetonium also will to be noted being kept perfectly, in order to avoid it is solid to cause the later stage normally not pollinate.After castration was finished, the thin kraft paper bag of utilization carried out the bagging isolation to the flower fringe and preserves moisture.
(3) manually get powder and pollination
At the 7:30 in the morning, the colored fringe bending that green stem based material Xiao Hua is just open is placed in the little glass, knocks the fringe body gently with pincet, allows paternal pollen be scattered in the little glass.To just fasten the pollen that takes off, dip in little writing brush that to brush red shank be that gynoecium stretches out on the coetonium column cap, pollinate continuously and just can guarantee that Hua Sui went up Xiao Hua and all awarded powder in 2 days from green stem.After each pollination, all the flower fringe is carried out bagging, until seed maturity, results with kraft paper bag.
(4) management after the hybridization
After hybridization is finished, the Xiao Hua that does not pluck is arranged, checked once colored fringe in per three days, check altogether five times, in time remove if find the Xiao Hua that newly grows for preventing red shank system pollination Hua Sui bottom.According to weather condition, checked once in general per five days, for the red shank based material is in time poured water, preserved moisture, fasten the ripe required moisture of hybrid seed to guarantee red shank, until seed maturity.
(5) results management
Gathered in the crops preceding 10 days at seed, seal the end opening of kraft paper bag with adhesive tape, in order to avoid seed comes off and can not receive seed.After treating that the flower fringe is withered and yellow fully, cut Hua Sui results seed.
2, hybrid identification
Be maternal with the centipede grass red shank in this example, green stem is a male parent, has obtained hybrid seed.In the strain cross materials that obtains, we have found that the plants stems look for green filial generation, illustrates that we have obtained real cross materials by the method.
Embodiment: 2
1, be that female parent, green stem are that male parent is carried out hybrid experiment with the centipede grass red shank.
(1) spend ear selection to select
On centipede grass red shank based material, select the existing a small amount of little tassel that the flowers are in blossom puts in top as the eunuchs fringe.
(2) artificial emasculation
Carried out in 6 o'clock in the afternoon, Xiao Hua and the unopened Xiao Hua in bottom that the tassel top of choosing is open extract in advance, only stay 20 small ears at the fringe middle part, under magnifying glass, utilize tweezers to extract all male flower on the stamen of open Xiao Hua and the fringe, to give special heed to gynoecium and the column cap of the Xiao Hua that to damage in the castration process, Xiao Hua is coetonium also will to be noted being kept perfectly, in order to avoid it is solid to cause the later stage normally not pollinate.After castration was finished, the thin kraft paper bag of utilization carried out the bagging isolation to the flower fringe and preserves moisture.
(3) manually get powder and pollination
At the 8:30 in the morning, the colored fringe bending that green stem based material Xiao Hua is just open is placed in the little glass, knocks the fringe body gently with pincet, allows paternal pollen be scattered in the little glass.To just fasten the pollen that takes off, dip in little writing brush that to brush red shank be that gynoecium stretches out on the coetonium column cap, pollinate continuously and just can guarantee that Hua Sui went up Xiao Hua and all awarded powder in 3 days from green stem.After each pollination, all the flower fringe is carried out bagging, until seed maturity, results with kraft paper bag.
(4) management after the hybridization
After hybridization is finished, the Xiao Hua that does not pluck is arranged, checked once colored fringe in per three days, check altogether five times, in time remove if find the Xiao Hua that newly grows for preventing red shank system pollination Hua Sui bottom.According to weather condition, checked once in general per five days, for the red shank based material is in time poured water, preserved moisture, fasten the ripe required moisture of hybrid seed to guarantee red shank, until seed maturity.
(5) results management
Gathered in the crops preceding 15 days at seed, seal the end opening of kraft paper bag with adhesive tape, in order to avoid seed comes off and can not receive seed.After treating that the flower fringe is withered and yellow fully, cut Hua Sui results seed.
2, hybrid identification
Be maternal with the centipede grass red shank in this example, green stem is a male parent, has obtained hybrid seed.In the plant cross materials that obtains, we have found that the plants stems look for green filial generation, illustrates that we have obtained real cross materials by the method.
Claims (5)
1. centipede grass cross-breeding method, comprise that colored ear selection is selected, management, results seed after artificial emasculation, artificial supplementary pollination and the pollination, it is characterized in that at centipede grass flowering habit and self-fruitful rate, set up the technical method of a cover centipede grass cross-breeding.
2. according to claim 1 centipede grass cross-breeding method, it is characterized in that described colored ear selection is selected to be meant, select the existing a small amount of little colored fringe that the flowers are in blossom puts in top.
3. according to claim 1 centipede grass cross-breeding method, it is characterized in that described artificial emasculation is meant, 4～6 o'clock in the afternoon, immature having spent for a short time of open Xiao Hua in the Hua Sui top of choosing and fringe bottom removed, under magnifying glass, extract the stamen that the fringe middle part remains Xiao Hua, castrate the back and carry out bagging with ox-hide pouch made of paper with tweezers.
4. according to claim 1 centipede grass cross-breeding method, it is characterized in that describedly manually getting powder and pollination is meant, 7:30～the 8:30 in morning next day that is castrating, collect the pollen of paternal plant with little glass, and the paternal pollen of collecting was gently brushed on the column cap of male flower of female parent fringe Xiao Hua with the fine, soft fur pen at 8～9 o'clock, carry out supplementary pollination.Still carry out bagging after the pollination with kraft paper bag.
5. according to claim 1-4 centipede grass cross-breeding method, it is characterized in that with the centipede grass red shank being maternal, green stem is a male parent, has obtained hybrid seed, has obtained the plants stems look and has been green filial generation.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA2007100232422A CN101116419A (en) | 2007-06-18 | 2007-06-18 | Centipede grass cross-breeding method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA2007100232422A CN101116419A (en) | 2007-06-18 | 2007-06-18 | Centipede grass cross-breeding method |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN101116419A true CN101116419A (en) | 2008-02-06 |
Family
ID=39052812
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNA2007100232422A Pending CN101116419A (en) | 2007-06-18 | 2007-06-18 | Centipede grass cross-breeding method |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN101116419A (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104823835A (en) * | 2015-04-30 | 2015-08-12 | 中南林业科技大学 | Biotechnological breeding platform for turfgrass |
| CN104855273A (en) * | 2015-05-21 | 2015-08-26 | 广西南亚热带农业科学研究所 | Artificial hybrid pollination tool for cassava |
| CN117327712A (en) * | 2023-10-24 | 2024-01-02 | 江苏省中国科学院植物研究所 | Method for regulating and controlling root growth of eremochloa ophiuroides through transferring EoBBR gene |
-
2007
- 2007-06-18 CN CNA2007100232422A patent/CN101116419A/en active Pending
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104823835A (en) * | 2015-04-30 | 2015-08-12 | 中南林业科技大学 | Biotechnological breeding platform for turfgrass |
| CN104855273A (en) * | 2015-05-21 | 2015-08-26 | 广西南亚热带农业科学研究所 | Artificial hybrid pollination tool for cassava |
| CN117327712A (en) * | 2023-10-24 | 2024-01-02 | 江苏省中国科学院植物研究所 | Method for regulating and controlling root growth of eremochloa ophiuroides through transferring EoBBR gene |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102524053B (en) | Seed production method for hybrid pepper variety | |
| CN102334445B (en) | Pollination method for purity-conservation Chinese cabbage breeding material | |
| CN106856966B (en) | A kind of hybrid selection method of photoperiod-sensitive type sudangrass | |
| CN103004583B (en) | Rapid purification method for cucumber female flower lines | |
| CN113785741A (en) | Seed production method for pepper breeding | |
| CN101697707B (en) | Method for cultivating amomum villosum | |
| CN106035055A (en) | Hybridization method for vigna radiata | |
| CN101116419A (en) | Centipede grass cross-breeding method | |
| CN111657141A (en) | Method for improving selfing pollination efficiency of corn bagging | |
| CN111567398A (en) | Malvaceae crop distant hybridization method | |
| CN104082129B (en) | A kind of pollinating method for pomegranate crossbreeding | |
| CN110074111A (en) | A kind of inorganic agent and method improving millet Cross fertile rate | |
| CN106818226B (en) | Multi-variety top-grafting head-changing hybrid seed production method for persimmon trees | |
| CN102742500B (en) | Artificial hybridization method for millet | |
| CN109122290A (en) | A kind of method of effective passivation Soviet Union gulf corn germplasm photoperiod sensitivity | |
| CN101731138A (en) | Compact corn seed producing method | |
| CN101473785B (en) | Bagged separated self-pollinated purified seed production method for dwarf coconut | |
| CN107853171A (en) | One kind food leaf grass and its implantation methods | |
| CN108739364B (en) | Method for improving seed production efficiency of self-incompatible gramineous plant hybridization | |
| CN107581056B (en) | A kind of papaya cross breeding method | |
| CN111264379A (en) | Banana cultivation method convenient for artificial pollination | |
| CN105359960A (en) | Populus cross breeding method | |
| CN111972282A (en) | Production method of cotton genic male sterile dual-purpose line hybrid seeds | |
| CN101595838A (en) | A kind of method of mango artificial hybridization pollination | |
| CN104885927A (en) | Natural pollination hybridizing method with castration for longan |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Open date: 20080206 |