CN104303993A - Breeding method for clonal DH33-27 variety of Eucalyptus urophylla*E.grandis - Google Patents
Breeding method for clonal DH33-27 variety of Eucalyptus urophylla*E.grandis Download PDFInfo
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- CN104303993A CN104303993A CN201410590344.2A CN201410590344A CN104303993A CN 104303993 A CN104303993 A CN 104303993A CN 201410590344 A CN201410590344 A CN 201410590344A CN 104303993 A CN104303993 A CN 104303993A
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Abstract
The invention discloses a breeding method for clonal DH33-27 variety of Eucalyptus urophylla*E.grandis. The method comprises the following steps: firstly, taking Eucalyptus urophylla U16 serving as a female parent and Eucalyptus grandis G33 serving as a male parent as parents; performing emasculation and bagging, pollen collection, hand pollination, cap bag removal, seed collecting and sowing and breeding to realize consistent character expression and stable inheritance. The clonal DH33-27 variety of Eucalyptus urophylla*E.grandis which is not subjected to segregation of character has the variety characteristics that the leaves are lanceolate, terminal buds are green, veins are obvious, seedling stems are slightly red, tree trunks in the early stage is brownish red, the upper parts of the tree trunks are cyan, and the tree trunks are completely peeled in the later period; the clonal DH33-27 variety of Eucalyptus urophylla*E.grandis has the characteristics of high general performance, straight stem shapes, order forest form, small branches, high annual average amount of growing stock and obvious economic benefit, is free of plant diseases and insect pests and suitable for large-scale cultivation promotion.
Description
Technical field
The present invention relates to field of plant breeding, particularly a kind of selection of tail alpine ash clone DH33-27 kind.
Background technology
China has many eucalypt specieses at present, as Eucalyptus urophylla, alpine ash, blue gum, eucalyptus camaldulensis, E. dunnii, Wang An etc., can produce multiple industrial timber; China enters Ecological Society, the strategy of sustainable development is subject to world's more and more national and public attention and accreditation, equally, the strategy of sustainable development of China's forestry is also instructing countries in the world development Modern Forestry, but is subject to the impact of eucalypt species, although China eucalyptus many kinds growing ability is strong, but because the felling cycle is short, instant eucalyptus regeneration capacity is strong, due to felling year after year and crop rotation, many eucalyptus growing areas are made to afforest not, severe water and soil erosion.Therefore, development of new eucalypt species, can ensure timber and other downstream links supply abundance, eucalyptus can be made again to accumulate biomass and promoted rapidly, within the short period, realize greening, the eucalyptus plantation for Subtropic of China area develops be about to face one new problem.
Inventor is through for many years to the research of eucalypt species seed selection work, and by working to the male parent hybridization of female parent seed selection of multiple eucalypt species, find that tail alpine ash is a kind of valuable economic forest, ecological forest kind, this arboreal growth phase is short, green hill can be made to afforest at three months to five months after plantation, within 6 years, can cut down, normal condition next mu of forest land can cut down 10 cubes; This tree is of many uses simultaneously: the timber that tail footpath is more than 25 centimetres, sells international market, can as fine paper raw material; Less than 25 centimeters, tail footpath can be used as plywood raw material; Diameter can be used as at the bottom of building mould at about 8 centimeters; Less also can be used as section, but how Breeding Traits is excellent, dimensionally stable, not easily producer sudden change, much remains to be done to meet again the eucalypt species of national strategy developing direction.
Summary of the invention
The object of this invention is to provide a kind of selection of tail alpine ash clone DH33-27 kind, to seek, a kind of fast grow is strong, biological accumulation amount is large, proterties is excellent and the eucalyptus new varieties of inheritance stability.
Technical scheme of the present invention is as follows:
Tail alpine ash clone DH33-27(
e. urophylla × E. grandis) selection of kind, with east gate Eucalyptus urophylla select tree U16(
eucalyptus urophylla) as maternal, using east gate alpine ash select tree G33 as male parent (
eucalyptus grandis); Obtaining the Characters through emasculation bagging, collection pollen, artificial pollination, removal bagging, seed collecting sowing and seed selection consistent, there is not the tail alpine ash clone DH33-27 kind of trait segregation in genetic stability.
Maternal east gate Eucalyptus urophylla select tree U16(
eucalyptus urophylla) originate in Indonesia, by forest farm, east gate, Guangxi, Eucalyptus urophylla Provenance Trial (E24) is set up to exotic tree, carry out seeds and individuality selects excellent, select maternal U16.
Male parent east gate alpine ash select tree G33(
eucalyptus grandis) draw from Australia, the seeds of being set up exotic tree respectively by forest farm, east gate, Guangxi and fertilizer test (E1), carry out seeds and individuality selects excellent, select male parent G33.
Parent selection criteria is: in progeny test test, general performance is excellent, form is logical straight, branch is little, forest form in good order, without damage by disease and insect.
Maternal east gate Eucalyptus urophylla select tree U16(
eucalyptus urophylla) feature: growth is fast, and trunk is logical straight, and bark surface is coarse, bottom rufous, and top canescence, branch is little.
Male parent east gate alpine ash select tree G33(
eucalyptus grandis) feature: growth is fast, and trunk is logical straight, and bark surface is smooth, bottom canescence, top dark green.
Select reason: Parent fast growing, individual volume are large, form is logical straight, and material is good, strong adaptability.
Described emasculation bagging is: selected 3 years these U16 of breeder mother of investigation are bloomed situation, blooms on a small quantity when observing tree has, most of bud, bud have green to become yellow-white and formed north lid come off ring time, carry out emasculation, while emasculation, carry out bagging isolation.In South China, Eucalyptus urophylla blossom phenophase, in annual 8-10 month, can be bloomed situation in investigating by the end of August maternal U16.
Described collection pollen is: selected 5 years these G33 of own father of investigation are bloomed situation, bloom on a small quantity when observing tree has, during major part bud, bud have green to become yellow-white and formed north lid come off ring time, artificial collection has the branch of bud, water planting 1-2 days in laboratory, pollen collecting is carried out in indoor, in drying basin, use silica dehydrator after 1 day the pollen collected, according to access times, that is: a secondary amounts can complete pollination 200-300 bud, proceed in dactylethrae, store front double-layer plastic bag to install, under being placed in low temperature shading environment, and sack of obturaging, prevent water suction, if short, storage temperature keeps 0-5 DEG C, long storage periods temperature remains on-20 DEG C ~-18 DEG C, and the improper meeting of Pollen preservation makes it lose activity, and takes out time to be used by usage amount.Described short is: period of storage is within 1 year, and described long storage periods is, period of storage was more than 1 year.In South China, alpine ash blossom phenophase, in annual 7-8 month, can be investigated male parent G33 in early July and to bloom situation.
Described artificial pollination is: after selected parent's bagging, remove bagging after 2-3 days, maternal column cap secretes transparent viscous liquid, now carries out first time pollination, puts cloth bag immediately after pollination; Second time pollination is carried out again after 2-3 days; During pollination, in dactylethrae, pick male parent flower pesticide with absorbent cotton, directly pollen is spread upon on maternal column cap, after pollination, put cloth bag immediately.
Described removal bagging is: in artificial pollination after 1 week, observes column cap and the style of pollination, until remove bagging after the dry blackening of column cap and style.
Described seed collecting sowing is: collect seed: in pollination after 90 days, and ovary surface across splits, and seed maturity can pluck seed; Sowing: mix seed and sterile fine earth thoroughly rear being evenly sprinkling upon on seedbed, seedbed builds as follows: with the pallet composition seedbed of 50cm × 50cm, tray bottom pad one deck newspaper, cultivate soil to be made up of the building ash of the grains of sand+20% of the yellow soil+30% of 50%, in advance with 1000-1500 times of liquid potassium permanganate by pallet and soil disinfection.Temperature requirement 26-30 DEG C, 5 hours sunshine, humidity 80%.
Described seed selection is: transplant when seed grows 3 pairs of true leaves on seedbed and cultivate to mud bag cup, the medium of mud bag cup kind consists of: 70% yellow soil+25% coal ash+5% perlite.Cultivate 1-2 month at mud bag cup, grow to 25-30cm high, afforest, set up the test of hybridization eucalyptus progeny test; Select hybrid generation defect individual, obtain fine individual plant DH33-27; Carry out cuttage and tissue culture propagation to this fine individual plant, and do 2-3 clonal test test, select choiceness DH33-27 eucalypt species, afforestation is produced in application, cumulative area 2000 mu.
Selection criteria: in clonal test test, general performance is excellent, form is logical directly, forest form is neat, branch is little, without damage by disease and insect, average annual accumulation is large, and economic benefit is obvious, is applicable to wide area popularizing planting.
The invention has the beneficial effects as follows:
(1) select original producton location Australia and Indonesia's eucalyptus provenance as parent, carry out afforestation seed selection in South China of China, by hybridizing the choiceness DH33-27 obtained, the Characters is consistent, and genetic stability, trait segregation does not occur.
(2) choiceness DH33-27 has following features: fast growing, rotation short (industrial fiber material); Form is logical directly, branch is little, forest form is neat; Material is good, and texture is straight, barren-resistant, is applicable to large area region plantation.
(3) the choiceness DH33-27 height of tree, tail footpath and year accumulation be maximum, mean stand height 24.63m, average tail footpath 18.27m, the accumulation of average per hectare reaches 57m
3/ hm
2, be the kind that clone tail alpine ash kind is given prominence to the most.
Embodiment
Inventor has carried out seed selection and the breeding work of eucalyptus new varieties of the present invention in eucalyptus forest farm, east gate, Guangxi, process is as follows:
(1) maternal: to introduce a collection of Indonesia provenance 8, in 3-4 month Raining season wooding 30 mu then, set up test E24 Eucalyptus urophylla Provenance Trial, investigates survival rate within one week and completes after-culture after afforestation;
Male parent: introduce a collection of Australian provenance 28, in 3-4 month Raining season wooding 35 mu then, the test E1 seeds of foundation and fertilizer test, investigates survival rate within one week and completes after-culture after afforestation.
Seeds are carried out and individuality selects excellent in the E24 provenance test set up, select maternal U16, male parent G33 is selected in the E1 seeds of having set up and fertilizer test, selection criteria: in provenance test contrast, kind of batch general performance is excellent, form logical straight, branch is little, forest form in good order, without damage by disease and insect, average annual accumulation is large, economic benefit is obvious, plantation suitable for large-scale promotion.
(2) emasculation bagging: to bloom situation in investigating 3 years selected these U16 of breeder mother by the end of August, bloom on a small quantity when observing tree has, most of bud, bud have green to become yellow-white and formed north lid come off ring time, carry out emasculation, while emasculation, carry out bagging isolation.
(3) pollen is gathered: to bloom situation in 5 years these G33 of own father that early July investigation is selected, bloom on a small quantity when observing tree has, during major part bud, bud have green to become yellow-white and formed north lid come off ring time, artificial collection has the branch of bud, water planting 1-2 days in laboratory, pollen collecting is carried out in indoor, in drying basin, use silica dehydrator after 1 day the pollen collected, according to access times, that is: a secondary amounts can complete pollination 200-300 bud, proceed in dactylethrae, store front double-layer plastic bag to install, under being placed in low temperature shading environment, and sack of obturaging, prevent water suction, if short, storage temperature keeps 0-5 DEG C, long storage periods temperature remains on-20 DEG C ~-18 DEG C, and the improper meeting of Pollen preservation makes it lose activity, and takes out time to be used by usage amount.
(4) artificial pollination: remove bagging after 2-3 days after selected parent's bagging, maternal column cap secretes transparent viscous liquid, now carries out first time pollination, puts cloth bag immediately after pollination; Second time pollination is carried out again after 2-3 days; During pollination, in dactylethrae, pick male parent flower pesticide with absorbent cotton, directly pollen is spread upon on maternal column cap, after pollination, put cloth bag immediately.
(5) remove bagging: after pollinating 1 week, observe column cap and the style of pollination, until remove bagging after the dry blackening of column cap and style.
(6) seed collecting sowing: collect seed: in pollination after 90 days, ovary surface across splits, and seed maturity can pluck seed; Sowing: mix seed and sterile fine earth thoroughly rear being evenly sprinkling upon on seedbed, seedbed builds as follows: with the pallet composition seedbed of 50cm × 50cm, tray bottom pad one deck newspaper, cultivate soil to be made up of the building ash of the grains of sand+20% of the yellow soil+30% of 50%, in advance with 1000-1500 times of liquid potassium permanganate by pallet and soil disinfection.Temperature requirement 26-30 DEG C, 5 hours sunshine, humidity 80%.
(7) seed selection: transplant when seed grows 3 pairs of true leaves on seedbed and cultivate to mud bag cup, the medium of mud bag cup kind consists of: 70% yellow soil+25% coal ash+5% perlite.Cultivate 1-2 month at mud bag cup, grow to 25-30cm high, afforest, set up the test of hybridization eucalyptus progeny test; Select hybrid generation defect individual, obtain fine individual plant DH33-27; Carry out cuttage and tissue culture propagation to this fine individual plant, and do 3 years clone comparative trials, in test, DH33-27 year Stock growth is in test had outstanding performance, and has potentiality to be exploited.Nineteen ninety-five starts to produce afforestation in Guangxi District, and the test gross area reaches 2000 mu, and we obtain genetic stability thus, and the tail alpine ash clone DH33-27(be separated does not occur proterties
e. urophylla × E. grandis) kind.This variety characteristic is: leaf lanceolar, and terminal bud is dark green, and vein is obvious, and seedling bar is partially red, early stage trunk brownish red, top cyan, and later stage decortication completely.
Inventor has carried out spread test to the tail alpine ash clone DH33-27 new varieties obtained in forest farm, east gate, Guangxi, and records the eucalyptus height of tree, the diameter of a cross-section of a tree trunk 1.3 meters above the ground and accumulation and calculate.
The raw eucalyptus Tree height growth of table 1:6.5 compares
Within 6.5 years, raw tail alpine ash clone DH33-27 and tail alpine ash family DH33 there is no the significance difference opposite sex on Tree height growth.In addition, the height of tree of DH33-27 kind reaches 24.66-25.11m, comparatively outstanding in tail alpine ash kind.
The raw eucalyptus breast diameter growth of table 2:6.5 compares
As can be seen from the table, 6.5 years raw tail alpine ash clone DH33-27 and tail alpine ash family DH33 there is no the significance difference opposite sex in breast diameter growth.Within 6.5 years, the raw tail alpine ash clone DH33-27 diameter of a cross-section of a tree trunk 1.3 meters above the ground reaches 18.27-18.33cm, and relative to other kind tail alpine ash, the diameter of a cross-section of a tree trunk 1.3 meters above the ground is maximum, and genetic character is also more stable, is reasonable fast-growing eucalypt species.
Table 3:3 life and 6.5 years raw accumulation growth fractions are comparatively
As can be seen from above-mentioned table, the merit in tail alpine ash clone DH33-27 new varieties clones heredity hybridization generation, within 3 years, accumulation reaches 123 m
3/ hm
2above, hybridizing family 3 years accumulations is 120m
3/ hm
2above; 6.5 years time, clone reaches as high as accumulation is 371.15 m
3/ hm
2, year accumulation reach 57.1
3/ hm
2, with hybridization generation without the significance difference opposite sex.
Claims (10)
1. a selection for tail alpine ash clone DH33-27 kind, is characterized in that: using east gate Eucalyptus urophylla select tree U16 as female parent, using east gate alpine ash select tree G33 as male parent; Obtaining the Characters through emasculation bagging, collection pollen, artificial pollination, removal bagging, seed collecting sowing and seed selection consistent, there is not the tail alpine ash clone DH33-27 kind of trait segregation in genetic stability.
2. the selection of tail alpine ash clone DH33-27 kind according to claim 1, it is characterized in that: described emasculation bagging is: selected 3 years these U16 of breeder mother of investigation are bloomed situation, bloom on a small quantity when observing tree has, major part bud, bud have green to become yellow-white and formed north lid come off ring time, carry out emasculation, while emasculation, carry out bagging isolation.
3. the selection of tail alpine ash clone DH33-27 kind according to claim 1, it is characterized in that: described collection pollen is: selected 5 years these G33 of own father of investigation are bloomed situation, bloom on a small quantity when observing tree has, during major part bud, bud have green to become yellow-white and formed north lid come off ring time, artificial collection has the branch of bud, water planting 1-2 days in laboratory, pollen collecting is carried out in indoor, in drying basin, use silica dehydrator after 1 day the pollen collected, according to access times, that is: a secondary amounts can complete pollination 200-300 bud, proceed in dactylethrae, store front double-layer plastic bag to install, under being placed in low temperature shading environment, and sack of obturaging, prevent water suction, if short, storage temperature keeps 0-5 DEG C, long storage periods temperature remains on-20 DEG C ~-18 DEG C, takes out time to be used by usage amount.
4. the selection of tail alpine ash clone DH33-27 kind according to claim 1, it is characterized in that: described artificial pollination is: remove bagging at selected parent's bagging after 2-3 days, maternal column cap secretes transparent viscous liquid, now carry out first time pollination, after pollination, put cloth bag immediately; Second time pollination is carried out again after 2-3 days; During pollination, in dactylethrae, pick male parent flower pesticide with absorbent cotton, directly pollen is spread upon on maternal column cap, after pollination, put cloth bag immediately.
5. the selection of tail alpine ash clone DH33-27 kind according to claim 1, is characterized in that: described removal bagging is: in artificial pollination after 1 week, observes column cap and the style of pollination, until remove bagging after the dry blackening of column cap and style.
6. the selection of tail alpine ash clone DH33-27 kind according to claim 1, is characterized in that: described seed collecting sowing is: collect seed: in pollination after 90 days, and ovary surface across splits, and seed maturity can pluck seed; Sowing: mix seed and sterile fine earth thoroughly rear being evenly sprinkling upon on seedbed, temperature requirement 26-30 DEG C, 5 hours sunshine, humidity 80%.
7. the selection of tail alpine ash clone DH33-27 kind according to claim 6, it is characterized in that: described seedbed builds as follows: with the pallet composition seedbed of 50cm × 50cm, tray bottom pad one deck newspaper, cultivate soil to be made up of the building ash of the grains of sand+20% of the yellow soil+30% of 50%, in advance with 1000-1500 times of liquid potassium permanganate by pallet and soil disinfection.
8. the selection of tail alpine ash clone DH33-27 kind according to claim 1, it is characterized in that: described seed selection is: when seed grows 3 pairs of true leaves on seedbed, transplanting to mud bag cup is cultivated, 1-2 month is cultivated at mud bag cup, grow to 25-30cm high, afforest, set up the test of hybridization eucalyptus progeny test; Select hybrid generation defect individual, obtain fine individual plant DH33-27; Carrying out cuttage and tissue culture propagation to this fine individual plant, and do 2-3 clone comparative trial, obtain genetic stability, there is not the tail alpine ash clone DH33-27 be separated in proterties.
9. the selection of tail alpine ash clone DH33-27 kind according to claim 8, is characterized in that: the medium of described mud bag cup kind consists of: 70% yellow soil+25% coal ash+5% perlite.
10. the tail alpine ash clone DH33-27 feature that method described in claim 1 selects is: leaf lanceolar, and terminal bud is dark green, and vein is obvious, and seedling bar is partially red, early stage trunk brownish red, top cyan, and later stage decortication completely.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105454047A (en) * | 2015-12-23 | 2016-04-06 | 广西壮族自治区国有东门林场 | Tissue culture rapid propagation method of eucalyptus cloeziana |
| CN105900829A (en) * | 2016-04-15 | 2016-08-31 | 中国林业科学研究院热带林业研究所 | Rapid breeding method of Eucapyptus urophylla + E. tereticornis artificial hydrids based on ion implantation |
| CN107258537A (en) * | 2017-06-27 | 2017-10-20 | 广西壮族自治区国有东门林场 | A kind of eucalyptus breeding tail eucalyptus camaldulensis DH191 4 tissue culture and rapid propagation method |
| CN107258536A (en) * | 2017-06-27 | 2017-10-20 | 广西壮族自治区国有东门林场 | A kind of eucalyptus breeding tail eucalyptus camaldulensis DH191 7 tissue culture and rapid propagation method |
-
2014
- 2014-10-29 CN CN201410590344.2A patent/CN104303993A/en active Pending
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105454047A (en) * | 2015-12-23 | 2016-04-06 | 广西壮族自治区国有东门林场 | Tissue culture rapid propagation method of eucalyptus cloeziana |
| CN105900829A (en) * | 2016-04-15 | 2016-08-31 | 中国林业科学研究院热带林业研究所 | Rapid breeding method of Eucapyptus urophylla + E. tereticornis artificial hydrids based on ion implantation |
| CN105900829B (en) * | 2016-04-15 | 2018-05-11 | 中国林业科学研究院热带林业研究所 | A kind of rapid breeding method based on the thin eucalyptus artificial hybridization kind of ion implanting tail |
| CN107258537A (en) * | 2017-06-27 | 2017-10-20 | 广西壮族自治区国有东门林场 | A kind of eucalyptus breeding tail eucalyptus camaldulensis DH191 4 tissue culture and rapid propagation method |
| CN107258536A (en) * | 2017-06-27 | 2017-10-20 | 广西壮族自治区国有东门林场 | A kind of eucalyptus breeding tail eucalyptus camaldulensis DH191 7 tissue culture and rapid propagation method |
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