ZA200607622B - Method for purifying plasmid DNA - Google Patents
Method for purifying plasmid DNA Download PDFInfo
- Publication number
- ZA200607622B ZA200607622B ZA200607622A ZA200607622A ZA200607622B ZA 200607622 B ZA200607622 B ZA 200607622B ZA 200607622 A ZA200607622 A ZA 200607622A ZA 200607622 A ZA200607622 A ZA 200607622A ZA 200607622 B ZA200607622 B ZA 200607622B
- Authority
- ZA
- South Africa
- Prior art keywords
- plasmid dna
- composition
- less
- dna
- chromatography
- Prior art date
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Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/10—Protozoa; Culture media therefor
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K48/00—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
- A61K48/0091—Purification or manufacturing processes for gene therapy compositions
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P35/00—Antineoplastic agents
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
- C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
- C12N15/101—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by chromatography, e.g. electrophoresis, ion-exchange, reverse phase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/26—Preparation of nitrogen-containing carbohydrates
- C12P19/28—N-glycosides
- C12P19/30—Nucleotides
- C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2303/00—Specific treatment goals
- C02F2303/06—Sludge reduction, e.g. by lysis
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| ZA200607622B true ZA200607622B (en) | 2008-05-28 |
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| ZA200607622A ZA200607622B (en) | 2004-04-19 | 2006-09-12 | Method for purifying plasmid DNA |
Country Status (16)
| Country | Link |
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| JP (2) | JP2007532123A (fr) |
| KR (1) | KR20060135062A (fr) |
| CN (2) | CN101006170A (fr) |
| AT (1) | ATE496990T1 (fr) |
| AU (2) | AU2005233309B2 (fr) |
| BR (1) | BRPI0509490A (fr) |
| CA (1) | CA2559368A1 (fr) |
| DE (1) | DE602005026113D1 (fr) |
| DK (1) | DK1737945T3 (fr) |
| EA (1) | EA010576B1 (fr) |
| MX (1) | MXPA06011568A (fr) |
| NO (1) | NO20065290L (fr) |
| NZ (1) | NZ549835A (fr) |
| WO (1) | WO2005100542A1 (fr) |
| ZA (1) | ZA200607622B (fr) |
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| CA2559368A1 (fr) * | 2004-04-19 | 2005-10-27 | Centelion | Procede destine a purifier de l'adn plasmidique |
| AU2008256681B2 (en) * | 2007-05-23 | 2014-07-31 | Vgxi, Inc | Compositions comprising high concentration of biologically active molecules and processes for preparing the same |
| KR20110028258A (ko) * | 2008-04-30 | 2011-03-17 | 그래댈리스, 인코포레이티드 | 고순도 플라스미드 ｄｎａ 제제 및 이의 제조방법 |
| US9149772B2 (en) | 2010-01-15 | 2015-10-06 | Board Of Regents, The University Of Texas Systems | Enhancing flux of a microporous hollow fiber membrane |
| US9688921B2 (en) | 2013-02-26 | 2017-06-27 | Board Of Regents, The University Of Texas System | Oil quality using a microporous hollow fiber membrane |
| US9643127B2 (en) | 2010-01-15 | 2017-05-09 | Board Of Regents Of The University Of Texas System | Simultaneous removal of oil and gases from liquid sources using a hollow fiber membrane |
| US8491792B2 (en) | 2010-01-15 | 2013-07-23 | Board Of Regents, The University Of Texas System | Non-dispersive process for insoluble oil recovery from aqueous slurries |
| US8617396B2 (en) | 2010-01-15 | 2013-12-31 | Board Of Regents, The University Of Texas System | Non-dispersive process for insoluble oil recovery from aqueous slurries |
| EP2523737A4 (fr) * | 2010-01-15 | 2015-10-14 | Univ Texas | Procédé non dispersif pour la récupération d'huile insoluble à partir de bouillies aqueuses |
| US9782726B2 (en) | 2010-01-15 | 2017-10-10 | Board Of Regents, The University Of Texas System | Non-dispersive process for oil recovery |
| US20130115598A1 (en) * | 2011-10-13 | 2013-05-09 | Lawrence Loeb | Oligonucleotide probe retrieval assay for dna transactions in mammalian cells |
| GB2497575A (en) * | 2011-12-15 | 2013-06-19 | Protein Technologies Ltd | Cell lysis in a flowing liquid using a lytic agent |
| WO2013188849A1 (fr) | 2012-06-14 | 2013-12-19 | Board Of Regents, The University Of Texas System | Récupération non-dispersive du pétrole à partir de sources liquides de l'industrie pétrolifère |
| EP2682168A1 (fr) | 2012-07-02 | 2014-01-08 | Millipore Corporation | Dispositif de tirage et métier à filer |
| CN104370997B (zh) * | 2014-09-24 | 2018-07-31 | 陈辉 | 去除生物制品中细菌内毒素的试剂盒、方法及其生物制品的制备方法 |
| CN106884011B (zh) * | 2015-12-16 | 2020-11-17 | 固安鼎泰海规生物科技有限公司 | 一种规模化质粒纯化的联合液相层析分离方法 |
| WO2017186815A1 (fr) * | 2016-04-26 | 2017-11-02 | Proqr Therapeutics Ii B.V. | Oligonucléotides antisens pour l'expression améliorée de la frataxine |
| AU2017284202B2 (en) * | 2016-06-14 | 2021-11-04 | Biogen Ma Inc. | Hydrophobic interaction chromatography for purification of oligonucleotides |
| CN106554956A (zh) * | 2016-12-07 | 2017-04-05 | 安徽智飞龙科马生物制药有限公司 | 一种产业化制备BCG‑CpG‑DNA的方法 |
| GB201710130D0 (en) | 2017-06-26 | 2017-08-09 | Ge Healthcare Bioprocess R & D Ab | A Method of seperation |
| JP6570795B2 (ja) * | 2017-08-25 | 2019-09-04 | 積水メディカル株式会社 | メチル化ｄｎａ分離及び／又は検出用クロマトグラフィー用充填剤 |
| CN108531563A (zh) * | 2018-02-05 | 2018-09-14 | 深圳市尚维高科有限公司 | 多孔微球的用途及裂解液以及裂解液的使用方法 |
| CN110348090B (zh) * | 2019-06-28 | 2021-05-04 | 浙江大学 | 基于人工神经网络实现多柱连续流层析设计及分析的方法 |
| CN111088248B (zh) * | 2019-12-23 | 2020-10-16 | 广州湾区生物科技有限公司 | 一种用于提取无内毒素质粒的磁性材料及其使用方法 |
| CN111394427B (zh) * | 2020-04-22 | 2023-04-11 | 宁波市博坤生物科技有限公司 | 一种含泥土检材中微量dna的提取方法 |
| CN112798697B (zh) * | 2020-11-16 | 2022-11-22 | 武汉波睿达生物科技有限公司 | 慢病毒包装系统辅助质粒的超螺旋结构纯度的检测方法 |
| CN115404154A (zh) * | 2021-05-10 | 2022-11-29 | 艾棣维欣(苏州)生物制品有限公司 | 用于提取细菌中质粒dna的装置 |
| CN115404232A (zh) * | 2021-05-10 | 2022-11-29 | 艾棣维欣(苏州)生物制品有限公司 | 用于提取细菌中质粒dna的方法 |
| CN114085828A (zh) * | 2021-11-17 | 2022-02-25 | 安徽荃银高科种业股份有限公司 | 一种水稻基因组dna的快速低成本提取方法及其应用 |
| WO2023095665A1 (fr) * | 2021-11-29 | 2023-06-01 | 東ソー株式会社 | Procédé d'isolement d'anticorps |
| EP4234695A1 (fr) * | 2022-02-24 | 2023-08-30 | Sartorius BIA Separations d.o.o. | Procédés d'élimination sélective de contaminants au cours d'un processus de purification d'agents biologiques |
| CN116987581A (zh) * | 2023-08-08 | 2023-11-03 | 江苏耀海生物制药有限公司 | 一种质粒dna制备装置及制备工艺 |
Family Cites Families (14)
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|---|---|---|---|---|
| US5561064A (en) * | 1994-02-01 | 1996-10-01 | Vical Incorporated | Production of pharmaceutical-grade plasmid DNA |
| FR2728264B1 (fr) * | 1994-12-16 | 1997-01-31 | Rhone Poulenc Rorer Sa | Purification d'adn par formation de triple helice avec un oligonucleotide immobilise |
| EP0811055B1 (fr) * | 1995-12-21 | 2004-06-09 | Genzyme Corporation | Procede de lyse de cellules |
| FR2746412B1 (fr) * | 1996-03-21 | 1998-06-12 | Rhone Poulenc Rorer Sa | Purification d'adn plasmidique de qualite pharmaceutique |
| US7026468B2 (en) * | 1996-07-19 | 2006-04-11 | Valentis, Inc. | Process and equipment for plasmid purification |
| US7807822B2 (en) * | 1996-08-01 | 2010-10-05 | Robert Bridenbaugh | Methods for purifying nucleic acids |
| FR2773818B1 (fr) * | 1998-01-21 | 2000-02-18 | Pasteur Merieux Serums Vacc | Procede et dispositif de lyse de bacteries |
| WO2000073318A1 (fr) * | 1999-05-28 | 2000-12-07 | Bio Science Contract Production Corporation | Procedes de purification d'adn et adn purifie |
| ES2298239T3 (es) * | 2000-05-26 | 2008-05-16 | Centelion | Purificacion de una formacion de triple helice con oligonucleotido inmovilizado. |
| EP1320741A4 (fr) * | 2000-09-18 | 2006-09-06 | Univ Washington | Dispositifs microfluidiques pour manipulation par rotation de l'interface fluidique entre plusieurs ecoulements fluidiques |
| US20040002081A1 (en) * | 2001-12-18 | 2004-01-01 | Boehringer Ingelheim International Gmbh And Bia Separations D.O.O. | Method and device for isolating and purifying a polynucleotide of interest on a manufacturing scale |
| JP4723254B2 (ja) * | 2002-12-23 | 2011-07-13 | バイカル インコーポレイテッド | プラスミドｄｎａを精製するための方法 |
| BRPI0413907A (pt) * | 2003-09-17 | 2006-10-24 | Centelion | método de preparação de plasmìdeo dna de grau farmacêutico |
| CA2559368A1 (fr) * | 2004-04-19 | 2005-10-27 | Centelion | Procede destine a purifier de l'adn plasmidique |
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2005
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- 2005-04-19 KR KR1020067023198A patent/KR20060135062A/ko not_active Application Discontinuation
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- 2005-04-19 DK DK05739527.9T patent/DK1737945T3/da active
- 2005-04-19 WO PCT/EP2005/005213 patent/WO2005100542A1/fr active Application Filing
- 2005-04-19 MX MXPA06011568A patent/MXPA06011568A/es active IP Right Grant
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Also Published As
| Publication number | Publication date |
|---|---|
| MXPA06011568A (es) | 2006-12-20 |
| JP2011132249A (ja) | 2011-07-07 |
| KR20060135062A (ko) | 2006-12-28 |
| CN102031258A (zh) | 2011-04-27 |
| WO2005100542A1 (fr) | 2005-10-27 |
| CN101006170A (zh) | 2007-07-25 |
| NO20065290L (no) | 2006-11-17 |
| JP2007532123A (ja) | 2007-11-15 |
| BRPI0509490A (pt) | 2007-09-11 |
| NZ549835A (en) | 2008-12-24 |
| DE602005026113D1 (de) | 2011-03-10 |
| AU2005233309A1 (en) | 2005-10-27 |
| ATE496990T1 (de) | 2011-02-15 |
| CA2559368A1 (fr) | 2005-10-27 |
| EA200601728A1 (ru) | 2007-04-27 |
| AU2005233309B2 (en) | 2011-03-31 |
| US20070213289A1 (en) | 2007-09-13 |
| AU2011200978A1 (en) | 2011-04-07 |
| DK1737945T3 (da) | 2011-05-09 |
| EA010576B1 (ru) | 2008-10-30 |
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| AU2005233309B2 (en) | Method for purifying plasmid DNA | |
| AU2004272748C1 (en) | Method of preparation of pharmaceutically grade plasmid DNA | |
| ES2359069T3 (es) | Método para purificar adn plásmido. | |
| CA2579340A1 (fr) | Preparations liquides stables d'adn plasmidique | |
| JP2006521105A (ja) | 生物分子の生成方法及び装置 | |
| EP1737945B1 (fr) | Procede destine a purifier de l'adn plasmidique | |
| ZA200602244B (en) | Method of preparation of pharmaceutically grade plasmid DNA | |
| O'Mahony et al. | Integration of Bacteria Capture via Filtration and in Situ Lysis for Recovery of Plasmid DNA under Industry‐Compatible Conditions | |
| Prazeres | Plasmid Purification |