WO2023016357A1 - 生物培育肉装置 - Google Patents

生物培育肉装置 Download PDF

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Publication number
WO2023016357A1
WO2023016357A1 PCT/CN2022/110462 CN2022110462W WO2023016357A1 WO 2023016357 A1 WO2023016357 A1 WO 2023016357A1 CN 2022110462 W CN2022110462 W CN 2022110462W WO 2023016357 A1 WO2023016357 A1 WO 2023016357A1
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Prior art keywords
tank
muscle
cell proliferation
medium
rotating shaft
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PCT/CN2022/110462
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English (en)
French (fr)
Inventor
王守伟
杨峰
李莹莹
李石磊
刘文婷
李雨爽
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中国肉类食品综合研究中心
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Application filed by 中国肉类食品综合研究中心 filed Critical 中国肉类食品综合研究中心
Priority to US18/031,903 priority Critical patent/US20230383223A1/en
Priority to GB2304113.0A priority patent/GB2613749A/en
Publication of WO2023016357A1 publication Critical patent/WO2023016357A1/zh

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    • C—CHEMISTRY; METALLURGY
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    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M21/00—Bioreactors or fermenters specially adapted for specific uses
    • C12M21/08—Bioreactors or fermenters specially adapted for specific uses for producing artificial tissue or for ex-vivo cultivation of tissue
    • A—HUMAN NECESSITIES
    • A22—BUTCHERING; MEAT TREATMENT; PROCESSING POULTRY OR FISH
    • A22C—PROCESSING MEAT, POULTRY, OR FISH
    • A22C7/00—Apparatus for pounding, forming, or pressing meat, sausage-meat, or meat products
    • A22C7/0092—Apparatus for pounding, forming, or pressing meat, sausage-meat, or meat products with worms or other rotary mounted members
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L13/00—Meat products; Meat meal; Preparation or treatment thereof
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L35/00—Food or foodstuffs not provided for in groups A23L5/00 – A23L33/00; Preparation or treatment thereof
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23P—SHAPING OR WORKING OF FOODSTUFFS, NOT FULLY COVERED BY A SINGLE OTHER SUBCLASS
    • A23P30/00—Shaping or working of foodstuffs characterised by the process or apparatus
    • A23P30/10—Moulding
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23P—SHAPING OR WORKING OF FOODSTUFFS, NOT FULLY COVERED BY A SINGLE OTHER SUBCLASS
    • A23P30/00—Shaping or working of foodstuffs characterised by the process or apparatus
    • A23P30/20—Extruding
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F27/00—Mixers with rotary stirring devices in fixed receptacles; Kneaders
    • B01F27/60—Mixers with rotary stirring devices in fixed receptacles; Kneaders with stirrers rotating about a horizontal or inclined axis
    • B01F27/72—Mixers with rotary stirring devices in fixed receptacles; Kneaders with stirrers rotating about a horizontal or inclined axis with helices or sections of helices
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    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/48—Holding appliances; Racks; Supports
    • C—CHEMISTRY; METALLURGY
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    • C12M25/00—Means for supporting, enclosing or fixing the microorganisms, e.g. immunocoatings
    • C12M25/06—Plates; Walls; Drawers; Multilayer plates
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    • C12M25/00—Means for supporting, enclosing or fixing the microorganisms, e.g. immunocoatings
    • C12M25/14—Scaffolds; Matrices
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    • C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
    • C12M27/02—Stirrer or mobile mixing elements
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    • C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
    • C12M27/18—Flow directing inserts
    • C12M27/20—Baffles; Ribs; Ribbons; Auger vanes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/04—Filters; Permeable or porous membranes or plates, e.g. dialysis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/18—External loop; Means for reintroduction of fermented biomass or liquid percolate
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
    • C12M41/18—Heat exchange systems, e.g. heat jackets or outer envelopes
    • C12M41/22—Heat exchange systems, e.g. heat jackets or outer envelopes in contact with the bioreactor walls
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
    • C12M41/32—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of substances in solution
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
    • C12M41/34—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of gas
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/42—Means for regulation, monitoring, measurement or control, e.g. flow regulation of agitation speed
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
    • C12M47/04—Cell isolation or sorting
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12M99/00—Subject matter not otherwise provided for in other groups of this subclass
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F2101/00—Mixing characterised by the nature of the mixed materials or by the application field
    • B01F2101/06—Mixing of food ingredients

Definitions

  • the invention relates to a biological cultivation meat device.
  • Biologically cultivated meat is the closest to traditional meat among all types of artificial meat. It has all the physical and chemical properties of real meat, which makes it have high commercial value. Although it is favored by the market, due to the lack of professional production equipment, the production method is still manual, which makes the production cost huge and the output is extremely low.
  • the present invention provides a device capable of simulating the growth environment of myoblasts, promoting the division and refusion of myoblasts, and finally processing them into meat products, so as to reduce production costs and increase production capacity.
  • the technical solution adopted by the present invention to solve its technical problems is: it comprises a culture medium regulating tank, a cell proliferation tank, a muscle separation tank, a compression molding device and a control system; Set the pipeline connecting the top, the cell proliferation tank is equipped with the medium inflow device connected to the medium regulating tank, a number of culture panels are arranged outside the pipeline, the culture panels are hinged to the pipeline, and the driving mechanism for turning the culture panel up and down around the hinge point is set on the pipeline.
  • the bottom of the cell proliferation tank is set in a funnel shape, the first muscle collection device connected to the cell proliferation tank is set in the pipeline, the second mixing slurry is set below the pipeline, and the temperature control heating device is set in the cell proliferation tank; the muscle collection device is connected to the muscle separation tank, A filter membrane layer is set in the muscle separation tank, a second muscle collection device connected to the compression molding device is arranged above the filter membrane layer, and a medium recovery device connected to the medium adjustment tank is arranged at the bottom of the muscle separation tank; the medium adjustment tank and the cell
  • the gas control device and the physical and chemical detection device of the culture medium are installed in the multiplication tank.
  • the culture panel includes a front panel, a rear panel, a rotating shaft and a bracket; the brackets are hinged on both sides of the pipeline, and the top of the cell proliferation tank is hinged to set a telescopic pull rod, and the output shaft of the telescopic pull rod is hinged to the first bracket below to cultivate up and down
  • the panels are connected by setting pull wires.
  • a rotating shaft is arranged on the bracket, and the front panel and the rear panel are arranged on the rotating shaft, and the shaft sleeve is arranged on the rotating shaft to realize coaxial reverse rotation through gear cooperation.
  • a muscle stirring bin is set in the compression molding device, and an extruding worm rotated by a third driving motor is set in the muscle stirring bin, and the left end of the muscle stirring bin is connected to a second muscle collecting device and an auxiliary material inlet is set, and an auxiliary material inlet is set at the muscle stirring bin.
  • the right end is provided with a molding die.
  • the beneficial effects of the present invention are: different from traditional manual production, the present invention controls the cultivation environment through automatic equipment, realizes the automatic integration from cultivation to finished products, increases production capacity, and reduces costs.
  • medium adjustment tank 1 cell proliferation tank 2, pipeline 21, medium inflow device 22, culture panel 23, pull wire 230, front panel 231, rear panel 232, rotating shaft 233, shaft sleeve 234, bracket 235, first Gear 236, second gear 237, third gear 238, connecting gear 239, umbrella baffle plate 24, second mixing paddle 25, second drive motor 251, flexible shaft 252, drive tooth 253, first muscle collection device 26, Vacuum interlayer 27, telescopic pull rod 28, drive motor 29, flexible shaft 291, muscle separation tank 3, compression molding device 4, second muscle collection device 41, muscle stirring chamber 42, third drive motor 43, extrusion worm 44, stirring Rod 45, auxiliary material inlet 46, forming mold 47, medium regulation system 51, cell proliferation control system 52, muscle collection and forming control system 53.
  • Biologically cultivated meat device of the present invention comprises medium adjustment tank 1, cell proliferation tank 2, muscle separation tank 3, compression molding device 4 and control system.
  • the control system is divided into a medium regulation system 51 for controlling the medium regulating tank 1, a cell proliferation control system 52 for controlling the cell proliferation tank 2, and a muscle collection and forming control system 53 for controlling the muscle separation tank 3 and the compression molding device 4.
  • the culture medium regulating tank 1 is provided with an injection port 10 for injecting culture medium, and is provided with a valve.
  • a first mixing paddle 11 for mixing the culture medium is installed in the medium adjustment tank 1, a pipe 21 connected to the top of the medium adjustment tank 1 is arranged in the cell proliferation tank 2, and a connection medium is arranged in the medium adjustment tank 1.
  • the medium flow into the device 22 of the tank 1 is regulated.
  • a plurality of cultivation panels 23 are arranged outside the pipeline 21, and the cultivation panels are hinged to the pipeline 21, and a driving mechanism for turning the cultivation panels 23 up and down around the hinge point is arranged on the pipeline 21.
  • the bottom of the cell proliferation tank 2 is set in a funnel shape to facilitate the collection of muscle tissue.
  • the second mixing slurry 25 is rotated under the pipeline 21 , responsible for the secondary mixing of the medium.
  • the bottom of the second mixing paddle 25 is rotated and arranged on the pipeline 21, and the bottom of the second mixing paddle 25 is provided with internal teeth in a ring shape, and the second driving motor 251 is used to drive a driving gear cooperating with the internal gears through a flexible shaft 252 253 to drive the second mixing paddle 25.
  • a first muscle collection device 26 connected to the cell proliferation tank 1 is arranged in the pipeline 21 .
  • the cell proliferation tank 2 is provided with a vacuum interlayer 27 for heat preservation, and is provided with a temperature-controlled heating device.
  • a gas control device 12 and a medium physical and chemical detection device 13 are arranged in the medium adjustment tank 1 and the cell proliferation tank 2.
  • the medium physical and chemical detection device 12 is responsible for detecting the condition of the medium, and controls the gas control device 12 and the temperature through a corresponding control system.
  • the control heating device makes corresponding adjustments to the internal temperature and gas.
  • the gas control device 12 in the cell proliferation tank 1 can detect data such as dissolved oxygen, carbon dioxide concentration and real-time pressure of the culture medium in real time and upload them to the cell proliferation control system 51 .
  • the first muscle collection device 26 is connected to the top in the muscle separation tank 3, and an umbrella-shaped baffle 24 is arranged at the bottom of the pipeline 21 to reduce the impact of the second mixed slurry 25 on the first collection device 26 at the bottom of the cell proliferation tank 2 .
  • a filter membrane for filtering muscle tissue is arranged in the muscle separation tank 3, and a protein membrane can be used as a filter membrane layer 31 to separate the muscle tissue from the culture medium.
  • the second muscle collection device 41 of the compression molding device 4 is set on the filter membrane layer 31
  • the medium recovery device 32 connected to the medium adjustment tank 1 is set at the bottom of the muscle separation tank 3 .
  • the muscle agitation bin 42 is set in the compression molding device 4, the extrusion worm 44 that is rotated by the third drive motor 43 is arranged in the muscle agitation bin 42, and the left side of the extrusion worm 44 is provided with a stirring rod 45,
  • the left end of the muscle stirring bin 42 is connected to the second muscle collecting device 41 and an auxiliary material inlet 46 for adding auxiliary materials such as pigments is set, and a molding die 47 is set at the right end of the muscle stirring bin 42 .
  • the extruding worm 44 rotates, the muscle tissue input by the second muscle collection device 41 into the muscle stirring chamber 42 will be gradually injected into the molding die 47 by the extruding worm 44 .
  • the culture medium inflow device 22 and the culture medium recovery device 32 are water pipes with a water pump, and the used water pipes are covered with micropores to reduce the impact on cells caused by excessive flow rates.
  • the first muscle collection device 26 and the second muscle collection device 41 are absorption ducts with centrifugal turbines.
  • the medium inflow device 22 and the medium recovery device 32 and the first muscle collection device 26 and the second muscle collection device 41 are respectively provided with flow regulating valves to control the size.
  • the culture panel 23 includes a front panel 231 , a rear panel 232 , a rotating shaft 233 , a shaft sleeve 234 and a bracket 235 .
  • the support 235 is hingedly arranged on both sides of the pipeline 21 , and the top of the cell multiplication tank 2 is hingedly provided with a telescopic pull rod 28 , and the output shaft of the telescopic pull rod 28 is hinged to the bracket 235 .
  • the rotating shaft 233 is set on the bracket 235, the front panel 231 and the rear panel 232 are rotatably arranged on the rotating shaft 233, the first gear 236 is respectively arranged on the front panel 231 and the rear panel 232, the axle sleeve 234 is rotatably arranged on the rotating shaft 233, the rotating shaft 233 and
  • the left and right sides of the shaft sleeve 234 are respectively provided with a second gear 237 and a third gear 238, the two second gears 237 mesh with the two first gears 236 respectively, and the two third gears 238 pass through the connecting gears arranged on the bracket 235 239 driving connection,
  • the top of the muscle proliferation tank 2 is provided with a driving motor 29, and the driving motor 29 drives and connects the rotating shaft 233 through a flexible shaft 291.
  • Up and down adjacent front panels 231 are connected to front panels 231 by pull wires 230 , and vertically adjacent rear panels 232 are also connected to each other by pull wires 230 .
  • the third gear 238 on the rotating shaft 233 and the shaft sleeve 234 will realize the coaxial reverse rotation through the connecting gear 239, during which the two second gears 237 on the rotating shaft 233 and the shaft sleeve 234 will be connected to the front panel 231 and the two first gears 236 on the rear panel 232 are meshed so that the front panel 231 and the rear panel 232 are folded in half up and down on the rotating shaft 233 .
  • the telescopic pull rod 28 between the bracket 235 and the cell proliferation tank 2 is stretched or stretched, so that the entire culture panel 2 is turned up and down around the hinge point of the bracket 235 and the pipeline 21 . Since the upper and lower adjacent cultivation panels 23 are connected by two pull wires 230, when the uppermost cultivation panel 23 is folded in half or reversed, the other cultivation panels 23 below will move synchronously.
  • the culture panel 23 is made of a material with good hydrophilicity and high biocompatibility, and other structures in contact with the muscle tissue and culture medium are all made of hydrophobic materials.
  • the present invention When the present invention is in use, first add the raw materials (PH buffer, serum, growth factor, myogenic factor and culture medium, etc.) for making biologically cultivated meat in the medium regulating tank in proportion, and then pass the first medium controlled by the medium regulating system.
  • the mixing paddle rotates the raw material to make the culture medium.
  • the medium is injected into the cell proliferation tank through the medium inflow device, and then the cell proliferation control system controls the cooperation of the temperature control heating device and the gas control device to simulate the biological environment in the body suitable for the proliferation of myoblasts, so that the cells in the medium Cells proliferate on culture panels.
  • the cell proliferation control system will control the stretching and drive the motor to fold the entire medium panel in half and then tilt it downwards, so that the cells gather, and the biological factors in the tank are adjusted through the medium to promote the fusion of myoblasts Myotubes are formed to further form muscle tissue, and then the muscle tissue slowly slides into the collection expansion, and then the muscle tissue is extracted into the muscle separation tank by the first muscle collection device.
  • the muscle tissue that enters the muscle separation tank will flow into the filtered medium through the filter membrane, so that the medium will flow into the medium adjustment tank through the medium recovery device, and the filtered muscle tissue will be sent to the compressor through the second muscle collection device.
  • seasoning and other auxiliary materials can be added to the auxiliary material inlet during the period.
  • the stirring rod at the left end of the rotating extrusion worm will mix the muscle tissue with the seasoning, and then use the worm to transfer the muscle tissue in the mixing chamber to the forming mold. Internal delivery, allowing the muscle tissue to be injected into the molding die and then extruded into a finished product.
  • the medium adjustment tank and the medium control system are responsible for adjusting the medium components in different cultured meat production stages, such as the delivery of pro-cell growth factors during the cell proliferation period, and the delivery of myogenic factors during the muscle fiber formation period to control the production of cultured meat
  • the culture medium after the secondary recovery will be removed toxins from cells, and new cytokines and cell culture components will be delivered to realize the repeated use of the culture medium and the regeneration of multiple applications.
  • the cell proliferation tank and the cell proliferation control system are responsible for promoting cell proliferation through the unfolded culture panel, and using microgravity to promote cell myotube fusion and muscle fiber generation through the folded culture panel, thereby promoting the production of muscle microtissue.
  • the muscle separation tank, compression molding device, and muscle collection and molding control system are responsible for the separation of the cultured meat microtissues from the culture medium, as well as the shaping, coloring, mixing and molding of the cultured meat microtissues, and finally make it a finished product of cultured meat.

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Abstract

生物培育肉装置，包括培养基调节罐（1）、细胞增殖罐（2）、肌肉分离罐（3）压缩成型装置（4）和控制系统。其中控制系统分为控制培养基调节罐（1）的培养基调控系统（51）、控制细胞增殖罐（2）的细胞增殖控制系统（52）和控制肌肉分离罐（3）与压缩成型装置（4）的肌肉收集成型控制系统（53）。区别传统于人工制作，所述装置通过自动化设备控制培养环境，实现培养到加工成品的自动一体化，提高产能，并降低成本。

Description

生物培育肉装置 技术领域
本发明涉及一种生物培育肉装置。
背景技术
生物培育肉是所有人造肉种类中与传统肉最为接近的，其具有真实肉类所有的理化性质，这使它具备很高的商用价值。虽然被市场看好，但由于没有专业的制作设备，制作方式还是由人工制作，使其生产成本巨大，且产量极低。
发明内容
针对上述现有技术的不足，本发明提供了一种能模拟成肌细胞生长环境，促进成肌细胞的分裂再融合，最终加工成肉制品的设备，来降低生产成本和提高产能。
本发明解决其技术问题所采用的技术方案是：它包括培养基调节罐、细胞增殖罐、肌肉分离罐、压缩成型装置和控制系统；培养基调节罐内设置第一混合浆，细胞增殖罐内设置连接顶部的管道，细胞增殖罐设置连接培养基调节罐的培养基流入装置，管道外排列设置若干培养面板，培养面板与管道铰接，管道上设置使培养面板绕铰接点上下翻转的驱动机构，细胞增殖罐的底部设置为漏斗状，管道内设置连接细胞增殖罐的第一肌肉收集装置，管道的下方设置第二混合浆，细胞增殖罐设置温控加热装置；肌肉收集装置连接肌肉分离罐，肌肉分离罐内设置滤膜层，在滤膜层的上方设置连接压缩成型装置的第二肌肉收集装置，肌肉分离罐的底部设置连接培养基调节罐的培养基回收装置；培养基调节罐和细胞增殖罐内设置气体控制装置和培养基理化检测装置。
进一步的，培养面板包括前面板、后面板、转轴和支架；支架铰接在管道的两侧，细胞增殖罐内的顶部铰接设置伸缩拉杆，伸缩拉杆的输出轴铰接下方的第一个支架，上下培养面板之间通过设置拉线连接。
进一步的，支架上设置转轴，转轴上转动设置前面板和后面板，转轴 上设置通过齿轮配合实现同轴反向转动的轴套，转轴与轴套分别和前面板与后面板啮合，细胞增殖罐设置驱动转轴的驱动电机。
进一步的，压缩成型装置内设置肌肉搅拌仓，肌肉搅拌仓内设置通过第三驱动电机转动的挤压蜗杆，肌肉搅拌仓的左端连接第二肌肉收集装置并设置一个辅料入口，在肌肉搅拌仓的右端设置成型模具。
本发明的有益效果是：区别传统于人工制作，本发明通过自动化设备控制培养环境，实现培养到加工成品的自动一体化，提高产能，并降低成本。
附图说明
附图1为本发明的整体结构示意图；
附图2为本发明的内部结构示意图；
附图3为细胞增殖罐被切开后的内部结构图；
附图4为培养面板的结构示意图；
附图5为培养面板的顶面剖视图。
图中：培养基调节罐1、细胞增殖罐2、管道21、培养基流入装置22、培养面板23、拉线230、前面板231、后面板232、转轴233、轴套234、支架235、第一齿轮236、第二齿轮237、第三齿轮238、连接齿轮239、伞状挡板24、第二混合浆25、第二驱动电机251、软轴252、驱动齿253、第一肌肉收集装置26、真空夹层27、伸缩拉杆28、驱动电机29、软轴291、肌肉分离罐3、压缩成型装置4、第二肌肉收集装置41、肌肉搅拌仓42、第三驱动电机43、挤压蜗杆44、搅拌杆45、辅料入口46、成型模具47、培养基调控系统51、细胞增殖控制系统52、肌肉收集成型控制系统53。
具体实施方式
为了更好地理解本发明，下面结合附图1至附图5来详细解释本发明的实施方式。
需要说明的是，下述的前后左右上下是以附图1中所示的前后左右上下为准的。
本发明的生物培育肉装置，包括培养基调节罐1、细胞增殖罐2、肌 肉分离罐3、压缩成型装置4和控制系统。其中控制系统分为控制培养基调节罐1的培养基调控系统51、控制细胞增殖罐2的细胞增殖控制系统52和控制肌肉分离罐3与压缩成型装置4的肌肉收集成型控制系统53。
如附图1和2中所示，培养基调节罐1设置用于注入培养基的注入口10，并设置阀门。培养基调节罐1内转动设置用于混合培养基的第一混合浆11，细胞增殖罐2内设置一根连接培养基调节罐1内顶部的管道21，培养基调节罐1内设置连接培养基调节罐1的培养基流入装置22。管道21外排列设置若干培养面板23，培养面板与管道21铰接，管道21上设置使培养面板23绕铰接点上下翻转的驱动机构。细胞增殖罐2的底部设置为漏斗状，方便肌肉组织汇集。
因为细胞增殖罐2内流入的培养基中接种细胞在不同位置，不同层次细胞的浓度不同，同时刚加入的细胞需要在培养基中混匀，所以在管道21的下方转动设置第二混合浆25，负责对培养基的二次混合。如附图3中所示，第二混合浆25转动设置管道21的底部，第二混合浆25底部环形设置内齿，利用第二驱动电机251通过软轴252驱动一个与内齿配合的驱动齿253来驱动第二混合浆25。设置在管道21内设置连接细胞增殖罐1的第一肌肉收集装置26。细胞增殖罐2设置保温用的真空夹层27，并设置温控加热装置。培养基调节罐1和细胞增殖罐2内设置气体控制装置12和培养基理化检测装置13，培养基理化检测装置12负责检测培养基的情况，并通过相应的控制系统控制气体控制装置12和温控加热装置对内部的温度和气体做出相应的调节。细胞增殖罐1内的气体控制装置12能实时检测培养基的溶氧量、二氧化碳浓度以及实时压强等数据并上传到细胞增殖控制系统51。
第一肌肉收集装置26连接肌肉分离罐3内的顶部，在管道21的底部设置一个伞状挡板24，用于减轻第二混合浆25对细胞增殖罐2的底部第一收集装置26的影响。
肌肉分离罐3内设置用于过滤肌肉组织的滤膜，可用蛋白膜做成滤膜层31，将肌肉组织和培养基分离。在滤膜层31上设置压缩成型装置4的第二肌肉收集装置41，在肌肉分离罐3的底部设置连接培养基调节罐1内的培养基回收装置32。如附图5中所示，压缩成型装置4内设置肌肉 搅拌仓42，肌肉搅拌仓42内设置通过第三驱动电机43转动的挤压蜗杆44，挤压蜗杆44的左侧设置搅拌杆45，肌肉搅拌仓42的左端连接第二肌肉收集装置41并设置一个用于添加色素等辅料的辅料入口46，在肌肉搅拌仓42的右端设置成型模具47。当挤压蜗杆44旋转时，第二肌肉收集装置41输入到肌肉搅拌仓42内的肌肉组织将被挤压蜗杆44逐渐注入成型模具47中。
培养基流入装置22和培养基回收装置32为带有水泵的水管，所用的水管内布满微孔，降低流速过大对细胞产生影响。第一肌肉收集装置26和第二肌肉收集装置41为带有离心涡轮的吸收管道。培养基流入装置22和培养基回收装置32与第一肌肉收集装置26和第二肌肉收集装置41分别设置控制大小的流量调节阀。
如附图3和4中所示，培养面板23包括前面板231、后面板232、转轴233、轴套234和支架235。支架235铰接设置在管道21的两侧，细胞增殖罐2内的顶部铰接设置伸缩拉杆28，伸缩拉杆28的输出轴铰接支架235。支架235上转动设置转轴233，前面板231和后面板232转动设置在转轴233上，前面板231和后面板232上分别设置第一齿轮236，轴套234转动设置在转轴233上，转轴233和轴套234的左右两侧分别设置第二齿轮237和第三齿轮238，两个第二齿轮237分别与两个第一齿轮236啮合，两个第三齿轮238通过设置在支架235上的连接齿轮239驱动连接，肌肉增殖罐2顶部设置驱动电机29，驱动电机29通过软轴291驱动连接转轴233。上下相邻的前面板231和前面板231之间通过拉线230连接，上下相邻的后面板232与后面板232之间也通过拉线230连接。
当转轴233旋转时，转轴233和轴套234上的第三齿轮238将通过连接齿轮239实现同轴反向旋转，期间转轴233和轴套234上的两个第二齿轮237将因为与前面板231和后面板232上的两个第一齿轮236的啮合，而使前面板231和后面板232在转轴233上实现上下对折。支架235与细胞增殖罐2之间的伸缩拉杆28在伸缩或撑开，使整个培养面板2绕支架235与管道21的铰接点上下翻转。由于上下相邻的培养面板23之间通过两根拉线230连接，当最上方的培养面板23对折或反转时，下方的其它培养面板23将同步运动。
培养面板23采用亲水性好，且生物相容性高的材料，其它与肌肉组织和培养基所接触的结构均采用疏水性材料制作。
本发明使用时，首先在培养基调节罐内按比例加入制作生物培育肉的原料(PH缓冲液、血清、生长因子、生肌因子和培养基等)，再通过培养基调控系统控制的第一混合浆旋转原料制作出培养基。然后通过培养基流入装置将培养基注入细胞增殖罐内，再由细胞增殖控制系统控制温控加热装置和气体控制装置的配合，模拟适宜成肌细胞增殖的机体内生物环境，使培养基中的细胞在培养面板上增殖。当增殖到一定量后，细胞增殖控制系统会控制伸缩和驱动电机将整个培养基面板对折后再向下倾斜，使细胞聚集，在通过培养基调节罐中的生物因子作用，促使成肌细胞融合成肌管，进一步形成肌肉组织，然后肌肉组织慢慢滑入收集扩，再由第一肌肉收集装置抽取肌肉组织到肌肉分离罐内。进入肌肉分离罐的肌肉组织将由滤膜一起流入的过滤培养基，使培养基通过培养基回收装置重新流入到培养基调节罐内，而过滤出的肌肉组织将通过第二肌肉收集装置输送到压缩成型装置的搅拌仓内，期间可在辅料入口内添加调味品等辅料，旋转的挤压蜗杆左端的搅拌杆会将肌肉组织和调味料混合，再利用蜗杆将搅拌仓内的肌肉组织向成型模具内输送，让肌肉组织注入成型模具后挤压成成品。
整个过程中，培养基调节罐和培养基控制系统负责调节不同培育肉制造时期的培养基成分，如对细胞增殖时期投递促细胞生长因子，肌纤维形成时期投递成肌因子等进行控制培育肉的制造过程；同时还将对二次回收后的培养基进行细胞内毒素去除，并投递新的细胞因子及细胞培养成分，实现培养基重复利用及可再生多次应用。
细胞增殖罐和细胞增殖控制系统负责通过展开的培养面板促进细胞的增殖，通过折叠后的培养面板利用微重力作用，促进细胞肌管融合及肌纤维生成，进而促进肌肉微组织制造。
肌肉分离罐、压缩成型装置和肌肉收集成型控制系统负责对培育肉微组织与培养基的分离，以及对培育肉微组织的塑形，调色，混合成型，最终使其成为培育肉成品。

Claims (4)

  1. 生物培育肉装置，其特征在于：它包括培养基调节罐、细胞增殖罐、肌肉分离罐、压缩成型装置和控制系统；培养基调节罐内设置第一混合浆，细胞增殖罐内设置连接顶部的管道，细胞增殖罐设置连接培养基调节罐的培养基流入装置，管道外排列设置若干培养面板，培养面板与管道铰接，管道上设置使培养面板绕铰接点上下翻转的驱动机构，细胞增殖罐的底部设置为漏斗状，管道内设置连接细胞增殖罐的第一肌肉收集装置，管道的下方设置第二混合浆，细胞增殖罐设置温控加热装置；肌肉收集装置连接肌肉分离罐，肌肉分离罐内设置滤膜层，在滤膜层的上方设置连接压缩成型装置的第二肌肉收集装置，肌肉分离罐的底部设置连接培养基调节罐的培养基回收装置；培养基调节罐和细胞增殖罐内设置气体控制装置和培养基理化检测装置。
  2. 如权利要求1中所述的生物培育肉装置，其特征在于：培养面板包括前面板、后面板、转轴和支架；支架铰接在管道的两侧，细胞增殖罐内的顶部铰接设置伸缩拉杆，伸缩拉杆的输出轴铰接下方的第一个支架，上下培养面板之间通过设置拉线连接。
  3. 如权利要求1或2中所述的生物培育肉装置，其特征在于：支架上设置转轴，转轴上转动设置前面板和后面板，转轴上设置通过齿轮配合实现同轴反向转动的轴套，转轴与轴套分别和前面板与后面板啮合，细胞增殖罐设置驱动转轴的第二驱动电机。
  4. 如权利要求1中所述的生物培育肉装置，其特征在于：压缩成型装置内设置肌肉搅拌仓，肌肉搅拌仓内设置通过第三驱动电机转动的挤压蜗杆，肌肉搅拌仓的左端连接第二肌肉收集装置并设置一个辅料入口，在肌肉搅拌仓的右端设置成型模具。
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