CN113604359B - 生物培育肉装置 - Google Patents

生物培育肉装置 Download PDF

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CN113604359B
CN113604359B CN202110906899.3A CN202110906899A CN113604359B CN 113604359 B CN113604359 B CN 113604359B CN 202110906899 A CN202110906899 A CN 202110906899A CN 113604359 B CN113604359 B CN 113604359B
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muscle
cell proliferation
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CN113604359A (zh
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王守伟
杨峰
李莹莹
李石磊
刘文婷
李雨爽
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China Meat Research Centre
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Abstract

本发明公开了生物培育肉装置，包括培养基调节罐、细胞增殖罐、肌肉分离罐、压缩成型装置和控制系统。其中控制系统分为控制培养基调节罐的培养基调控系统、控制细胞增殖罐的细胞增殖控制系统和控制肌肉分离罐与压缩成型装置的肌肉收集成型控制系统。区别传统于人工制作，本发明通过自动化设备控制培养环境，实现培养到加工成品的自动一体化，提高产能，并降低成本。

Description

生物培育肉装置
技术领域
本发明涉及一种生物培育肉装置。
背景技术
生物培育肉是所有人造肉种类中与传统肉最为接近的，其具有真实肉类所有的理化性质，这使它具备很高的商用价值。虽然被市场看好，但由于没有专业的制作设备，制作方式还是由人工制作，使其生产成本巨大，且产量极低。
发明内容
针对上述现有技术的不足，本发明提供了一种能模拟成肌细胞生长环境，促进成肌细胞的分裂再融合，最终加工成肉制品的设备，来降低生产成本和提高产能。
本发明解决其技术问题所采用的技术方案是：它包括培养基调节罐、细胞增殖罐、肌肉分离罐、压缩成型装置和控制系统；培养基调节罐内设置第一混合浆，细胞增殖罐内设置连接顶部的管道，细胞增殖罐设置连接培养基调节罐的培养基流入装置，管道外排列设置若干培养面板，培养面板与管道铰接，管道上设置使培养面板绕铰接点上下翻转的驱动机构，细胞增殖罐的底部设置为漏斗状，管道内设置连接细胞增殖罐的第一肌肉收集装置，管道的下方设置第二混合浆，细胞增殖罐设置温控加热装置；肌肉收集装置连接肌肉分离罐，肌肉分离罐内设置滤膜层，在滤膜层的上方设置连接压缩成型装置的第二肌肉收集装置，肌肉分离罐的底部设置连接培养基调节罐的培养基回收装置；培养基调节罐和细胞增殖罐内设置气体控制装置和培养基理化检测装置。
进一步的，培养面板包括前面板、后面板、转轴和支架；支架铰接在管道的两侧，细胞增殖罐内的顶部铰接设置伸缩拉杆，伸缩拉杆的输出轴铰接下方的第一个支架，上下培养面板之间通过设置拉线连接。
进一步的，支架上设置转轴，转轴上转动设置前面板和后面板，转轴上设置通过齿轮配合实现同轴反向转动的轴套，转轴与轴套分别和前面板与后面板啮合，细胞增殖罐设置驱动转轴的驱动电机。
进一步的，压缩成型装置内设置肌肉搅拌仓，肌肉搅拌仓内设置通过第三驱动电机转动的挤压蜗杆，肌肉搅拌仓的左端连接第二肌肉收集装置并设置一个辅料入口，在肌肉搅拌仓的右端设置成型模具。
本发明的有益效果是：区别传统于人工制作，本发明通过自动化设备控制培养环境，实现培养到加工成品的自动一体化，提高产能，并降低成本。
附图说明
附图1为本发明的整体结构示意图；
附图2为本发明的内部结构示意图；
附图3为细胞增殖罐被切开后的内部结构图；
附图4为培养面板的结构示意图；
附图5为培养面板的顶面剖视图。
图中：培养基调节罐1、细胞增殖罐2、管道21、培养基流入装置22、培养面板23、拉线230、前面板231、后面板232、转轴233、轴套234、支架235、第一齿轮236、第二齿轮237、第三齿轮238、连接齿轮239、伞状挡板24、第二混合浆25、第二驱动电机251、软轴252、驱动齿253、第一肌肉收集装置26、真空夹层27、伸缩拉杆28、驱动电机29、软轴291、肌肉分离罐3、压缩成型装置4、第二肌肉收集装置41、肌肉搅拌仓42、第三驱动电机43、挤压蜗杆44、搅拌杆45、辅料入口46、成型模具47、培养基调控系统51、细胞增殖控制系统52、肌肉收集成型控制系统53。
具体实施方式
为了更好地理解本发明，下面结合附图1至附图5来详细解释本发明的实施方式。
需要说明的是，下述的前后左右上下是以附图1中所示的前后左右上下为准的。
本发明的生物培育肉装置，包括培养基调节罐1、细胞增殖罐2、肌肉分离罐3、压缩成型装置4和控制系统。其中控制系统分为控制培养基调节罐1的培养基调控系统51、控制细胞增殖罐2的细胞增殖控制系统52和控制肌肉分离罐3与压缩成型装置4的肌肉收集成型控制系统53。
如附图1和2中所示，培养基调节罐1设置用于注入培养基的注入口10，并设置阀门。培养基调节罐1内转动设置用于混合培养基的第一混合浆11，细胞增殖罐2内设置一根连接培养基调节罐1内顶部的管道21，培养基调节罐1内设置连接培养基调节罐1的培养基流入装置22。管道21外排列设置若干培养面板23，培养面板与管道21铰接，管道21上设置使培养面板23绕铰接点上下翻转的驱动机构。细胞增殖罐2的底部设置为漏斗状，方便肌肉组织汇集。
因为细胞增殖罐2内流入的培养基中接种细胞在不同位置，不同层次细胞的浓度不同，同时刚加入的细胞需要在培养基中混匀，所以在管道21的下方转动设置第二混合浆25，负责对培养基的二次混合。如附图3中所示，第二混合浆25转动设置管道21的底部，第二混合浆25底部环形设置内齿，利用第二驱动电机251通过软轴252驱动一个与内齿配合的驱动齿253来驱动第二混合浆25。设置在管道21内设置连接细胞增殖罐1的第一肌肉收集装置26。细胞增殖罐2设置保温用的真空夹层27，并设置温控加热装置。培养基调节罐1和细胞增殖罐2内设置气体控制装置12和培养基理化检测装置13，培养基理化检测装置12负责检测培养基的情况，并通过相应的控制系统控制气体控制装置12和温控加热装置对内部的温度和气体做出相应的调节。细胞增殖罐1内的气体控制装置12能实时检测培养基的溶氧量、二氧化碳浓度以及实时压强等数据并上传到细胞增殖控制系统51。
第一肌肉收集装置26连接肌肉分离罐3内的顶部，在管道21的底部设置一个伞状挡板24，用于减轻第二混合浆25对细胞增殖罐2的底部第一收集装置26的影响。
肌肉分离罐3内设置用于过滤肌肉组织的滤膜，可用蛋白膜做成滤膜层31，将肌肉组织和培养基分离。在滤膜层31上设置压缩成型装置4的第二肌肉收集装置41，在肌肉分离罐3的底部设置连接培养基调节罐1内的培养基回收装置32。如附图5中所示，压缩成型装置4内设置肌肉搅拌仓42，肌肉搅拌仓42内设置通过第三驱动电机43转动的挤压蜗杆44，挤压蜗杆44的左侧设置搅拌杆45，肌肉搅拌仓42的左端连接第二肌肉收集装置41并设置一个用于添加色素等辅料的辅料入口46，在肌肉搅拌仓42的右端设置成型模具47。当挤压蜗杆44旋转时，第二肌肉收集装置41输入到肌肉搅拌仓42内的肌肉组织将被挤压蜗杆44逐渐注入成型模具47中。
培养基流入装置22和培养基回收装置32为带有水泵的水管，所用的水管内布满微孔，降低流速过大对细胞产生影响。第一肌肉收集装置26和第二肌肉收集装置41为带有离心涡轮的吸收管道。培养基流入装置22和培养基回收装置32与第一肌肉收集装置26和第二肌肉收集装置41分别设置控制大小的流量调节阀。
如附图3和4中所示，培养面板23包括前面板231、后面板232、转轴233、轴套234和支架235。支架235铰接设置在管道21的两侧，细胞增殖罐2内的顶部铰接设置伸缩拉杆28，伸缩拉杆28的输出轴铰接支架235。支架235上转动设置转轴233，前面板231和后面板232转动设置在转轴233上，前面板231和后面板232上分别设置第一齿轮236，轴套234转动设置在转轴233上，转轴233和轴套234的左右两侧分别设置第二齿轮237和第三齿轮238，两个第二齿轮237分别与两个第一齿轮236啮合，两个第三齿轮238通过设置在支架235上的连接齿轮239驱动连接，肌肉增殖罐2顶部设置驱动电机29，驱动电机29通过软轴291驱动连接转轴233。上下相邻的前面板231和前面板231之间通过拉线230连接，上下相邻的后面板232与后面板232之间也通过拉线230连接。
当转轴233旋转时，转轴233和轴套234上的第三齿轮238将通过连接齿轮239实现同轴反向旋转，期间转轴233和轴套234上的两个第二齿轮237将因为与前面板231和后面板232上的两个第一齿轮236的啮合，而使前面板231和后面板232在转轴233上实现上下对折。支架235与细胞增殖罐2之间的伸缩拉杆28在伸缩或撑开，使整个培养面板2绕支架235与管道21的铰接点上下翻转。由于上下相邻的培养面板23之间通过两根拉线230连接，当最上方的培养面板23对折或反转时，下方的其它培养面板23将同步运动。
培养面板23采用亲水性好，且生物相容性高的材料，其它与肌肉组织和培养基所接触的结构均采用疏水性材料制作。
本发明使用时，首先在培养基调节罐内按比例加入制作生物培育肉的原料(PH缓冲液、血清、生长因子、生肌因子和培养基等)，再通过培养基调控系统控制的第一混合浆旋转原料制作出培养基。然后通过培养基流入装置将培养基注入细胞增殖罐内，再由细胞增殖控制系统控制温控加热装置和气体控制装置的配合，模拟适宜成肌细胞增殖的机体内生物环境，使培养基中的细胞在培养面板上增殖。当增殖到一定量后，细胞增殖控制系统会控制伸缩和驱动电机将整个培养基面板对折后再向下倾斜，使细胞聚集，在通过培养基调节罐中的生物因子作用，促使成肌细胞融合成肌管，进一步形成肌肉组织，然后肌肉组织慢慢滑入收集扩，再由第一肌肉收集装置抽取肌肉组织到肌肉分离罐内。进入肌肉分离罐的肌肉组织将由滤膜一起流入的过滤培养基，使培养基通过培养基回收装置重新流入到培养基调节罐内，而过滤出的肌肉组织将通过第二肌肉收集装置输送到压缩成型装置的搅拌仓内，期间可在辅料入口内添加调味品等辅料，旋转的挤压蜗杆左端的搅拌杆会将肌肉组织和调味料混合，再利用蜗杆将搅拌仓内的肌肉组织向成型模具内输送，让肌肉组织注入成型模具后挤压成成品。
整个过程中，培养基调节罐和培养基控制系统负责调节不同培育肉制造时期的培养基成分，如对细胞增殖时期投递促细胞生长因子，肌纤维形成时期投递成肌因子等进行控制培育肉的制造过程；同时还将对二次回收后的培养基进行细胞内毒素去除，并投递新的细胞因子及细胞培养成分，实现培养基重复利用及可再生多次应用。
细胞增殖罐和细胞增殖控制系统负责通过展开的培养面板促进细胞的增殖，通过折叠后的培养面板利用微重力作用，促进细胞肌管融合及肌纤维生成，进而促进肌肉微组织制造。
肌肉分离罐、压缩成型装置和肌肉收集成型控制系统负责对培育肉微组织与培养基的分离，以及对培育肉微组织的塑形，调色，混合成型，最终使其成为培育肉成品。

Claims (1)

1.生物培育肉装置，其特征在于：它包括培养基调节罐、细胞增殖罐、肌肉分离罐、压缩成型装置和控制系统；培养基调节罐内设置第一混合桨，细胞增殖罐内设置连接顶部的管道，细胞增殖罐设置连接培养基调节罐的培养基流入装置，管道外排列设置若干培养面板，培养面板与管道铰接，管道上设置使培养面板绕铰接点上下翻转的驱动机构，细胞增殖罐的底部设置为漏斗状，管道内设置连接细胞增殖罐的第一肌肉收集装置，管道的下方转动设置第二混合桨，细胞增殖罐设置温控加热装置；第一肌肉收集装置连接肌肉分离罐的顶部，肌肉分离罐内设置滤膜层，在滤膜层的上方设置连接压缩成型装置的第二肌肉收集装置，肌肉分离罐的底部设置连接培养基调节罐的培养基回收装置；培养基调节罐和细胞增殖罐内设置气体控制装置和培养基理化检测装置；
培养面板包括前面板、后面板、转轴和支架；支架铰接在管道的两侧，细胞增殖罐内的顶部铰接设置伸缩拉杆，伸缩拉杆的输出轴铰接下方的第一个支架，上下培养面板之间通过设置拉线连接；
支架上设置转轴，转轴上转动设置前面板和后面板，转轴上设置通过齿轮配合实现同轴反向转动的轴套，转轴与轴套分别和前面板与后面板啮合，细胞增殖罐设置驱动转轴的第二驱动电机；
压缩成型装置内设置肌肉搅拌仓，肌肉搅拌仓内设置通过第三驱动电机转动的挤压蜗杆，肌肉搅拌仓的左端连接第二肌肉收集装置并设置一个辅料入口，在肌肉搅拌仓的右端设置成型模具。
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