WO2022244877A1 - エンベロープを有さないウイルスの付着が抑制された器具 - Google Patents

エンベロープを有さないウイルスの付着が抑制された器具 Download PDF

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WO2022244877A1
WO2022244877A1 PCT/JP2022/020992 JP2022020992W WO2022244877A1 WO 2022244877 A1 WO2022244877 A1 WO 2022244877A1 JP 2022020992 W JP2022020992 W JP 2022020992W WO 2022244877 A1 WO2022244877 A1 WO 2022244877A1
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group
virus
carbon atoms
coating film
linear
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PCT/JP2022/020992
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English (en)
French (fr)
Japanese (ja)
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一隆 的場
宏之 中嶋
泰斗 西野
祐大 菅原
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日産化学株式会社
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Priority to JP2023522742A priority Critical patent/JPWO2022244877A1/ja
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    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08F—MACROMOLECULAR COMPOUNDS OBTAINED BY REACTIONS ONLY INVOLVING CARBON-TO-CARBON UNSATURATED BONDS
    • C08F220/00—Copolymers of compounds having one or more unsaturated aliphatic radicals, each having only one carbon-to-carbon double bond, and only one being terminated by only one carboxyl radical or a salt, anhydride ester, amide, imide or nitrile thereof
    • C08F220/02—Monocarboxylic acids having less than ten carbon atoms; Derivatives thereof
    • C08F220/10—Esters
    • C08F220/12—Esters of monohydric alcohols or phenols
    • C—CHEMISTRY; METALLURGY
    • C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
    • C09D—COATING COMPOSITIONS, e.g. PAINTS, VARNISHES OR LACQUERS; FILLING PASTES; CHEMICAL PAINT OR INK REMOVERS; INKS; CORRECTING FLUIDS; WOODSTAINS; PASTES OR SOLIDS FOR COLOURING OR PRINTING; USE OF MATERIALS THEREFOR
    • C09D201/00—Coating compositions based on unspecified macromolecular compounds
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M1/00—Apparatus for enzymology or microbiology
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage

Definitions

  • the present invention relates to a device with suppressed virus adhesion, a method for reducing virus adhesion, a virus test kit, and a method for lowering the detection limit of virus tests.
  • Non-Patent Document 1 samples such as body fluids and sewage are collected, stored in a container, and then the virus is measured.
  • the virus may adhere to the surface of the storage container, causing the disappearance of the virus and a decrease in recoverability, which has been a problem.
  • proteins also reduce recovery and make stable detection difficult due to adsorption on the container surface. Materials have been reported (see, for example, Patent Document 1). Additives such as surfactants may improve the adsorption of proteins to the container surface, while surfactants destroy certain types of viruses. The problem was that there was a problem.
  • the purpose of the present invention is to provide an instrument with suppressed virus adhesion, a method for reducing virus adhesion, a virus test kit, and a method for improving virus test sensitivity.
  • the present invention includes the following.
  • the coating film contains a polymer of a monomer having a hydrophilic functional group.
  • the hydrophilic functional group is selected from phosphoric acid, phosphonic acid and ester structures thereof; betaine structure; amide structure; alkylene glycol residue; amino group; .
  • the coating film comprises a repeating unit containing a group represented by the following formula (a), a repeating unit containing a group represented by the following formula (b), and a group represented by the following formula (c)
  • a copolymer comprising a repeating unit comprising: [In the formula, U a1 , U a2 , U b1 , U b2 and U b3 each independently represent a hydrogen atom or a linear or branched alkyl group having 1 to 5 carbon atoms; R c is a linear or branched alkyl group having 4 to 18 carbon atoms, a cyclic hydrocarbon group having 3 to 10 carbon atoms, an aryl group having 6 to 10 carbon atoms, and an aralkyl group having 7 to 14 carbon atoms.
  • the virus is selected from Reoviridae, Caliciviridae, Picornaviridae, Astroviridae, Hepeviridae, Parvoviridae, Polyomaviridae, Papillomaviridae, Adenoviridae, and is adeno-associated 2.
  • [6] A method for reducing virus adhesion using the device according to any one of [1] to [5].
  • [7] A virus test kit comprising the device according to any one of [1] to [5].
  • [8] The device according to any one of [1] to [5], which is a virus storage container.
  • [9] A method for reducing the detection limit of a virus test using the device according to any one of [1] to [5].
  • a device with suppressed virus attachment and a method for reducing virus attachment using the device can be provided.
  • a virus storage container with less loss and a virus test kit with improved virus detection sensitivity can be provided.
  • the virus storage container of the present application also has the effect of maintaining virus infectivity (infectivity of virus) even after storage for a certain period of time.
  • the "virus” is a “non-enveloped virus (excluding adeno-associated virus)", which will be described later in detail. Non-enveloped viruses (excluding adeno-associated viruses)”.
  • halogen atom means a fluorine atom, a chlorine atom, a bromine atom or an iodine atom.
  • an "alkyl group” means a linear or branched saturated aliphatic hydrocarbon monovalent group.
  • Examples of the "straight or branched alkyl group having 1 to 5 carbon atoms” include, for example, methyl group, ethyl group, n-propyl group, isopropyl group, n-butyl group, isobutyl group, s-butyl group, t-butyl group, n-pentyl group, 1-methylbutyl group, 2-methylbutyl group, 3-methylbutyl group, 1,1-dimethylpropyl group, 1,2-dimethylpropyl group, 2,2-dimethylpropyl group or 1-ethylpropyl groups.
  • linear or branched alkyl group having 1 to 18 carbon atoms examples include, in addition to examples of “linear or branched alkyl group having 1 to 5 carbon atoms", hexyl group, heptyl group, octyl group, nonyl group, decyl group, undecyl group, dodecyl group, tridecyl group, tetradecyl group, pentadecyl group, hexadecyl group, heptadecyl group, octadecyl group, or isomers thereof.
  • the "linear or branched alkyl group having 1 to 5 carbon atoms which may be substituted with a halogen atom” means the above linear or branched alkyl group having 1 to 5 carbon atoms, or It means a linear or branched alkyl group having 1 to 5 carbon atoms, substituted with one or more halogen atoms. Examples of the “linear or branched alkyl group having 1 to 5 carbon atoms" are as described above.
  • a "linear or branched alkyl group having 1 to 5 carbon atoms substituted with one or more halogen atoms” means that one or more arbitrary hydrogen atoms of the above linear or branched alkyl group having 1 to 5 carbon atoms are , is substituted with a halogen atom, examples include fluoromethyl group, difluoromethyl group, trifluoromethyl group, chloromethyl group, dichloromethyl group, trichloromethyl group, bromomethyl group, iodomethyl group, 2, 2,2-trifluoroethyl group, 2,2,2-trichloroethyl group, perfluoroethyl group, perfluorobutyl group, perfluoropentyl group and the like.
  • ether linkage means -O-.
  • a linear or branched alkylene group having 1 to 10 carbon atoms which may be substituted with a halogen atom means a linear or branched alkylene group having 1 to 10 carbon atoms, or one or more halogen atoms. It means a linear or branched alkylene group having 1 to 10 carbon atoms substituted with .
  • alkylene group means a divalent organic group corresponding to the above alkyl group.
  • linear or branched alkylene group having 1 to 10 carbon atoms examples include methylene group, ethylene group, propylene group, trimethylene group, tetramethylene group, 1-methylpropylene group, 2-methylpropylene group, dimethylethylene group, ethylethylene group, pentamethylene group, 1-methyl-tetramethylene group, 2-methyl-tetramethylene group, 1,1-dimethyl-trimethylene group, 1,2-dimethyl-trimethylene group, 2,2-dimethyl- trimethylene group, 1-ethyl-trimethylene group, hexamethylene group, octamethylene group and decamethylene group, and the like.
  • ethylene group, propylene group, octamethylene group and decamethylene group are preferable.
  • a linear or branched alkylene group having 1 to 5 carbon atoms such as a propylene group, a trimethylene group and a tetramethylene group is more preferable, and an ethylene group or a propylene group is particularly preferable.
  • a linear or branched alkylene group having 1 to 10 carbon atoms substituted with one or more halogen atoms means that one or more arbitrary hydrogen atoms in the above alkylene group are replaced with halogen atoms. In particular, those in which some or all of the hydrogen atoms in the ethylene group or propylene group are replaced with halogen atoms are preferred.
  • a "cyclic hydrocarbon group having 3 to 10 carbon atoms” means one of monocyclic or polycyclic, saturated or partially unsaturated aliphatic hydrocarbons having 3 to 10 carbon atoms. means a valence group.
  • a monocyclic or bicyclic saturated aliphatic hydrocarbon monovalent group having 3 to 10 carbon atoms is preferable, for example, 3 carbon atoms such as cyclopropyl, cyclobutyl or cyclohexyl to 10 cycloalkyl groups, or bicycloalkyl groups having 4 to 10 carbon atoms such as bicyclo[3.2.1]octyl group, bornyl group and isobornyl group.
  • the "aryl group having 6 to 10 carbon atoms” means a monocyclic or polycyclic aromatic hydrocarbon monovalent group having 6 to 10 carbon atoms, such as phenyl group, naphthyl group or anthryl group.
  • the “aryl group having 6 to 10 carbon atoms” may be substituted with one or more of the above “linear or branched alkyl groups having 1 to 5 carbon atoms which may be substituted with halogen atoms”.
  • the "aralkyl group having 7 to 14 carbon atoms” is the group -R-R' (wherein R represents the above “linear or branched alkylene group having 1 to 5 carbon atoms", R ' represents the above-mentioned "aryl group having 6 to 10 carbon atoms”), and examples thereof include a benzyl group, a phenethyl group, an ⁇ -methylbenzyl group, and the like.
  • the aryl moiety of the "aralkyl group having 7 to 14 carbon atoms” may be substituted with one or more of the above "linear or branched alkyl groups having 1 to 5 carbon atoms which may be substituted with halogen atoms". good.
  • the "aryloxyalkyl group having 7 to 14 carbon atoms” is the group -R-O-R' (where R is the above “linear or branched alkylene group having 1 to 5 carbon atoms” and R′ represents the above “aryl group having 6 to 10 carbon atoms”), and examples thereof include a phenoxymethyl group, a phenoxyethyl group, a phenoxypropyl group, and the like.
  • the aryl moiety of the "aryloxyalkyl group having 7 to 14 carbon atoms” is substituted with one or more of the above "linear or branched alkyl groups having 1 to 5 carbon atoms which may be substituted with halogen atoms”.
  • halide ion means fluoride ion, chloride ion, bromide ion or iodide ion.
  • inorganic acid ions mean carbonate ions, sulfate ions, phosphate ions, hydrogen phosphate ions, dihydrogen phosphate ions, nitrate ions, perchlorate ions or borate ions.
  • An 2 - are halide ions, sulfate ions, phosphate ions, hydroxide ions and isothiocyanate ions, and halide ions are particularly preferred.
  • (meth)acrylate compounds mean both acrylate compounds and methacrylate compounds.
  • (meth)acrylic acid means acrylic acid and methacrylic acid.
  • the non-enveloped device of the present application with reduced virus attachment has a hydrophilic coating film on at least a portion of the surface.
  • a hydrophilic coating film is, for example, a static contact angle measurement using a contact angle meter (for example, fully automatic contact angle meter (Kyowa Interface Chemical Co., Ltd., DM-701)), in water (room temperature, for example, 25 ⁇ 5°C) is 140° or more, preferably 150° or more.
  • a contact angle meter for example, fully automatic contact angle meter (Kyowa Interface Chemical Co., Ltd., DM-701)
  • room temperature for example, 25 ⁇ 5°C
  • room temperature for example, 25 ⁇ 5°C
  • the hydrophilic coating film may be provided on at least a part of the surface of the device described later, but it is preferable that the coating film is formed over the entire surface that can come into contact with the virus, and the entire surface of the device is coated. It is more preferable that a film is formed.
  • the hydrophilic coating film contains a polymer of a monomer having a hydrophilic functional group.
  • a polymer of a monomer having a hydrophilic functional group according to the present invention may be a polymer of an ethylenically unsaturated monomer having a hydrophilic functional group or structure, or a polysaccharide or derivative thereof.
  • ethylenically unsaturated monomers include one or more ethylenically unsaturated monomers selected from the group consisting of (meth)acrylic acid and its esters; vinylpyrrolidone; and ethylene.
  • polysaccharides or derivatives thereof include cellulosic polymers such as hydroxyalkylcellulose (eg, hydroxyethylcellulose or hydroxypropylcellulose), starch, dextran, and curdlan.
  • hydrophilic functional group i.e., hydrophilic functional group or structure
  • hydrophilic functional group is selected from phosphoric acid, phosphonic acid and ester structures thereof; betaine structure; amide structure; alkylene glycol residue; is preferred.
  • a betaine structure means a monovalent or divalent group of compounds having an amphoteric center with a quaternary ammonium type cationic structure and an acidic anionic structure, such as the phosphorylcholine group: can be mentioned.
  • ethylenically unsaturated monomers having such a structure include 2-methacryloyloxyethylphosphorylcholine (MPC).
  • the amide structure has the formula: [Here, R 16 , R 17 and R 18 are each independently a hydrogen atom or an organic group (e.g., a methyl group, a hydroxymethyl group, a hydroxyethyl group, etc.)] means a group represented by Examples of ethylenically unsaturated monomers having such a structure include (meth)acrylamide, N-(hydroxymethyl)(meth)acrylamide, and the like. Furthermore, monomers or polymers having such a structure are disclosed in, for example, JP-A-2010-169604.
  • An alkylene glycol residue is an alkylene glycol (HO-Alk-OH; where Alk is a linear or branched alkylene group having 1 to 10 carbon atoms), one or both terminal hydroxyl groups of which are condensed with another compound. It means an alkyleneoxy group (--Alk--O--) that remains after reacting, and also includes poly(alkyleneoxy) groups in which alkyleneoxy units are repeated.
  • alkyleneoxy group --Alk--O---
  • Examples of ethylenically unsaturated monomers having such structures include 2-hydroxyethyl (meth)acrylate, methoxypolyethylene glycol (meth)acrylate, and the like. Further, monomers or polymers having such structures are disclosed in, for example, JP-A-2008-533489.
  • An amino group has the formula: —NH 2 , —NHR 19 or —NR 20 R 21 [wherein R 19 , R 20 and R 21 are each independently an organic group (for example, linear or branched alkyl group, etc.)].
  • Amino groups in the present invention include quaternary or salified amino groups. Examples of ethylenically unsaturated monomers having such a structure include dimethylaminoethyl (meth)acrylate, 2-(t-butylamino)ethyl (meth)acrylate, methacryloylcholine chloride, and the like.
  • a sulfinyl group has the formula: [Here, R 22 is an organic group (eg, an organic group having 1 to 10 carbon atoms, preferably an alkyl group having 1 to 10 carbon atoms and having one or more hydroxy groups, etc.)] means a group represented by Examples of polymers having such a structure include copolymers disclosed in Japanese Unexamined Patent Application Publication No. 2014-48278.
  • the polymer contained in the coating film of the present invention comprises a repeating unit containing a group represented by the following formula (a), a repeating unit containing a group represented by the following formula (b), and a repeating unit represented by the following formula (c). It may be a copolymer containing a repeating unit containing a group represented by: [In the formula, U a1 , U a2 , U b1 , U b2 and U b3 each independently represent a hydrogen atom or a linear or branched alkyl group having 1 to 5 carbon atoms; R c is a linear or branched alkyl group having 4 to 18 carbon atoms, a cyclic hydrocarbon group having 3 to 10 carbon atoms, an aryl group having 6 to 10 carbon atoms, and an aralkyl group having 7 to 14 carbon atoms.
  • an aryloxyalkyl group having 7 to 14 carbon atoms (wherein the aryl moiety may be substituted with a linear or branched alkyl group having 1 to 5 carbon atoms which may be substituted with a halogen atom ) represents;
  • An ⁇ represents an anion selected from the group consisting of halide ions, inorganic acid ions, hydroxide ions and isothiocyanate ions].
  • the coating film of the present invention is formed by applying a known hydrophilic coating film-forming composition to at least a part of the surface of the device described later, preferably the surface of the device that can come into contact with the virus, more preferably the surface of the device. can be formed by coating the entire surface of the by a known method.
  • the description of the hydrophilic coating film is as described above.
  • the device having the hydrophilic coating film of the present invention on at least a part of its surface preferably comprises a repeating unit containing a group represented by the following formula (a) and a group represented by the following formula (b): and a repeating unit containing a group represented by the following formula (c):
  • U a1 , U a2 , U b1 , U b2 and U b3 each independently represent a hydrogen atom or a linear or branched alkyl group having 1 to 5 carbon atoms ; a linear or branched alkyl group having 4 to 18 carbon atoms, a cyclic hydrocarbon group having 3 to 10 carbon atoms, an aryl group having 6 to 10 carbon atoms, an aralkyl group having 7 to 14 carbon atoms or an aralkyl group having 7 to 14 carbon atoms; represents an aryloxyalkyl group of 14 (wherein said aryl moiety is optionally substituted with a linear or branched alky
  • the copolymer contained in the coating film-forming composition comprises a repeating unit containing a group represented by formula (a) above and a group represented by formula (b) above. and a repeating unit containing the group represented by the above formula (c).
  • the repeating unit containing the group represented by the formula (c) includes repeating units containing the group represented by the formula (a) and repeating units containing the group represented by the formula (b). Different from units.
  • the polymer comprises a monomer containing a group represented by formula (a) above, a monomer containing a group represented by formula (b) above, and a monomer containing a group represented by formula (c) above.
  • copolymers include olefin-reacted vinyl polymer polymers, polyamides, polyesters, polycarbonates, polyurethanes, etc. Among them, olefin-reacted vinyl polymer polymers or (meth)acrylate compounds are particularly polymerized. (Meth)acrylic polymers are preferred.
  • the proportion of the repeating unit containing the group represented by formula (a) in the copolymer of the coating film of the present invention is 3 mol% to 80 mol%, preferably 3.5 mol% to 50 mol%. and more preferably 4 mol % to 30 mol %.
  • the copolymer according to the present invention may contain repeating units containing two or more groups represented by formula (a).
  • the proportion of repeating units containing the group represented by formula (b) in the copolymer of the coating film of the present invention is 3 mol% to 80 mol%, preferably 5 mol% to 70 mol%. , more preferably 8 mol % to 65 mol %.
  • the copolymer according to the present invention may contain repeating units containing two or more groups represented by formula (b).
  • the proportion of the repeating unit containing the group represented by the formula (c) in the copolymer according to the present invention may be the entire remainder obtained by subtracting the above formulas (a) and (b) from the total copolymer. , the remainder after subtracting the total ratio of the above formulas (a) and (b) and the fourth component described below, for example, 1 mol% to 90 mol%, preferably 3 mol% to 88 mol %. More preferably, it ranges from 5 mol % to 87 mol %. Most preferably it is 50 mol % to 86 mol %.
  • the copolymer according to the present invention may contain repeating units containing two or more groups represented by formula (c).
  • Copolymers containing repeating units of the following formulas (a1), (b1) and (c1) are particularly preferably used as the copolymer contained in the coating film-forming composition.
  • T a , T b , T c , U a1 , U a2 , U b1 , U b2 and U b3 each independently represents a hydrogen atom or a linear or branched alkyl group having 1 to 5 carbon atoms.
  • Q a and Q b each independently represent a single bond, an ester bond or an amide bond
  • Q c represents a single bond, an ether bond or an ester bond
  • R a and R b each independently represents a linear or branched alkylene group having 1 to 10 carbon atoms which may be substituted with a halogen atom
  • R c is a linear or branched alkyl group having 4 to 18 carbon atoms
  • An - represents a halide ion, an inorganic acid ion, a hydroxide ion and an isothiocyanate
  • m represents an integer of 0 to 6, preferably an integer of 1 to 6, more preferably an integer of 1 to 5, and particularly preferably 1.
  • the ratio of the copolymer containing the repeating units of the formulas (a1), (b1) and (c1) is, respectively, the above formulas (a), (b) and (c) in the copolymer according to the present invention. ) is the same as described above for the proportion of repeating units containing the group represented by ).
  • the above copolymers have the following formulas (A), (B) and (C): [In the formula, T a , T b , T c , U a1 , U a2 , U b1 , U b2 and U b3 each independently represents a hydrogen atom or a linear or branched alkyl group having 1 to 5 carbon atoms; Q a and Q b each independently represent a single bond, an ester bond or an amide bond, and Q c represents a single bond, an ether bond or an ester bond; R a and R b each independently represents a linear or branched alkylene group having 1 to 10 carbon atoms which may be substituted with a halogen atom, and R c is a linear chain having 4 to 18 carbon atoms or a branched alkyl group, a cyclic hydrocarbon group having 3 to 10 carbon atoms, an aryl group having 6 to 10 carbon atoms, an aralkyl group having 7
  • Ta, Tb and Tc are preferably a hydrogen atom, a methyl group or an ethyl group, more preferably a hydrogen atom or a methyl group.
  • U a1 , U a2 , U b1 , U b2 and U b3 are preferably a hydrogen atom, a methyl group, an ethyl group or a t-butyl group .
  • U b1 , U b2 and U b3 in b) are more preferably a hydrogen atom, a methyl group, an ethyl group or a t-butyl group.
  • the copolymer may further contain units derived from an optional fourth component.
  • the fourth component may contain a crosslinked structure derived from a (meth)acrylate compound having two or more functional groups.
  • a fourth component include ethylene glycol di(meth)acrylate, triethylene glycol di(meth)acrylate, propylene glycol di(meth)acrylate, bis(methacryloyloxymethyl) phosphate, bis[(2 -methacryloyloxy)ethyl], bis[3-(methacryloyloxy)propyl] phosphate, phosphiniridin tris(oxy-2,1-ethanediyl) triacrylate, and the like.
  • the ratio of the crosslinked structure derived from the (meth)acrylate compound having two or more functional groups in the copolymer is 0 mol% to 50 mol%, preferably 5 mol% to 45 mol%. and most preferably 10 mol % to 40 mol %.
  • the coating film-forming composition is applied to at least part of the surface of the instrument.
  • the coating method is not particularly limited, and common coating methods such as spin coating, dip coating, and solvent casting are used.
  • a method for obtaining a device having a coating film of the present invention may include a drying step of the coating film following the coating step described above.
  • the drying step of the coating film is carried out in the atmosphere or under vacuum, preferably at a temperature in the range of -200°C to 200°C.
  • the drying step removes the solvent in the coating film-forming composition, and the copolymers of the formulas (a) and (b) of the present invention form ionic bonds and are completely fixed to the container.
  • the coating film can be formed, for example, by drying at room temperature (10° C. to 35° C., for example, 25° C.). good too. Further, a drying step at extremely low to low temperatures (around -200°C to -30°C) by a freeze-drying method may be used.
  • Freeze-drying which is called vacuum freeze-drying, is a method in which a substance to be dried is usually cooled with a refrigerant and the solvent is removed by sublimation in a vacuum state.
  • Common refrigerants used in freeze-drying include a mixed medium of dry ice and methanol (-78°C), liquid nitrogen (-196°C), and the like.
  • drying temperature is -200°C or lower, an uncommon refrigerant must be used, which lacks versatility, and drying takes a long time due to solvent sublimation, resulting in poor efficiency. If the drying temperature is 200° C. or higher, the ionic bond reaction on the surface of the coating film proceeds too much, the surface loses hydrophilicity, and the ability to suppress virus adhesion is not exhibited. A more preferable drying temperature is 10°C to 180°C, and a more preferable drying temperature is 25°C to 150°C.
  • At least one selected from aqueous solutions containing water and electrolytes is added.
  • a step of washing with a solvent may be performed. Cleaning is preferably performed with running water, ultrasonic cleaning, or the like.
  • the aqueous solution containing the water and the electrolyte may be heated, for example, in the range of 40.degree. C. to 95.degree.
  • Aqueous solutions containing electrolytes are preferably PBS, physiological saline (containing only sodium chloride), Dulbecco's phosphate-buffered saline, Tris-buffered physiological saline, HEPES-buffered physiological saline, and Veronal-buffered physiological saline, and PBS is preferred. Especially preferred.
  • the coating film After being fixed, the coating film is not eluted even if it is washed with water, PBS, alcohol, etc., and remains firmly fixed to the substrate. Even if a virus adheres to the formed coating film, it can be easily removed by washing with water or the like.
  • treatment with radiation, electron beam, ethylene oxide, autoclave, etc. may be performed for sterilization.
  • the film thickness of the coating film of the present invention is preferably 10-1000 ⁇ , more preferably 10-500 ⁇ , most preferably 20-400 ⁇ .
  • the instrument of the present invention has a coating film formed from the above coating agent on at least part of the surface of the instrument. Specifically, the coating film is provided on the surface of the device that can come into contact with the virus, more preferably on the entire surface of the device.
  • the surface of the instrument may be subjected to a known plasma treatment.
  • a method of UV irradiation or oxygen plasma treatment is known to make the surface of oxides such as glass and ITO (Indium Tin Oxide) hydrophilic.
  • ITO Indium Tin Oxide
  • plasma includes active charged particles and active radicals such as vacuum plasma generated by oxygen-, nitrogen-, and fluorine-based single gases or mixed gases thereof, or plasma generated under atmospheric pressure or near atmospheric pressure. can be generated using a device capable of creating a space in which is present at a high density.
  • viruses In general, viruses (including vaccines and viral vectors) are broadly classified into enveloped and non-enveloped types.
  • An envelope is a membranous structure composed of lipids and proteins derived from host cells and glycoproteins derived from viruses, and there are viruses with and without envelopes.
  • virus means "non-enveloped virus (excluding adeno-associated virus)" unless otherwise specified.
  • Non-enveloped viruses include Reoviridae, Caliciviridae , Picornaviridae, Astroviridae, Hepeviridae, Parvoviridae, Polyomaviridae, Papillomaviridae, and Adenoviridae, and specific examples include reovirus, rotavirus, Colorado tick fever virus, No. Walk virus, Sapporo virus, polio virus, Coxsackie virus group A, Coxsackie virus group B, enterovirus, rhinovirus, hepatitis A virus, astrovirus, hepatitis E virus, B19 virus, JC virus, Hiropaviroma virus, feline calicivirus and Human adenovirus is included. These vaccine uses and vector uses are also included in the rights of the present application. One or a combination of two or more of these viruses is not excluded from the viruses referred to in this application.
  • non-enveloped viruses referred to in the present invention are not limited to those that are infectious or pathogenic to humans.
  • Non-enveloped viruses that are infectious or pathogenic to animals other than humans or plants are not excluded from the non-enveloped viruses referred to in the present application.
  • the device of the present invention is not particularly limited as long as it is used for non-enveloped viruses, but when used, it is required to be in contact with the virus and to suppress the attachment of the virus. is desirable.
  • the shape is also not particularly limited, such as flat plate shape, curved surface shape, uneven shape, and the like.
  • microwell plates include microwell plates, microplates, microtubes, chips, culture flasks, biodevices, syringes, prefilled syringes, filters, non-woven fabrics, vials, etc., which usually have multiple wells (depressions).
  • the storage container preferably has the aforementioned coating film on the virus-contacting surface.
  • the shape of the virus storage container is not particularly limited, such as a bottle shape or a tube shape, as long as it can store a virus-containing solution (such as an aqueous solution containing a virus, which is normally liquid at room temperature). It is preferable that the container has a lid or the like that can be tightly closed so that the container can be stored in a sealed state.
  • the material of the instrument of the present invention is also not particularly limited. Glass, metal-containing compounds, semi-metal-containing compounds, or resins can be used, but from the viewpoint of versatility, glass or resin moldings are preferably used.
  • Metal-containing compounds or semi-metal-containing compounds are, for example, ceramics, which are sintered bodies whose basic components are metal oxides and are sintered by heat treatment at high temperatures, semiconductors such as silicon, metal oxides or semi-metal oxides (silicon oxides, alumina, etc.), metal carbides or semi-metal carbides, metal nitrides or semi-metal nitrides (silicon nitride, etc.), metal borides or semi-metal borides, inorganic solid materials such as moldings of inorganic compounds, aluminum , nickel titanium, and stainless steel (SUS304, SUS316, SUS316L, etc.).
  • the resin may be a natural resin or a derivative thereof, or a synthetic resin.
  • the natural resin or a derivative thereof include cellulose, cellulose triacetate (CTA), nitrocellulose (NC), cellulose with immobilized dextran sulfate, and synthetic resins.
  • Resins include polyacrylonitrile (PAN), polyimide (PI), polyester polymer alloy (PEPA), polystyrene (PS), polysulfone (PSF), polyethylene terephthalate (PET), polymethyl methacrylate (PMMA), polyvinyl alcohol (PVA).
  • polyurethane PU
  • EVAL ethylene vinyl alcohol
  • PE polyethylene
  • PET polyester
  • PP polypropylene
  • PVDF polyvinylidene fluoride
  • PES polyethersulfone
  • PC polycarbonate
  • COP cycloolefin polymer
  • ZEONOR registered trademark
  • ZEONEX registered trademark
  • PVC polyvinyl chloride
  • PTFE polytetrafluoroethylene
  • UHPE ultra-high molecular weight polyethylene
  • PDMS polydimethylsiloxane
  • ABS acrylonitrile-butadiene-styrene resin
  • Teflon registered trademark
  • the method for reducing virus adhesion of the present invention is a method that includes the step of contacting a virus with the above-mentioned device, thereby reducing virus adherence to the device.
  • a virus-containing solution is placed in the container, and after storage for a certain period of time, the amount of virus in the solution changes little from the initial amount (for example, the change from the initial amount is 30%) within).
  • the certain period of time is, for example, one hour to one year.
  • the temperature may be frozen (eg -100°C to -20°C or below), refrigerated (eg below -20°C to below 10°C), or room temperature (eg below 10°C to 35°C).
  • the virus in the solution is, for example, 50% or more, 60% or more, 70% or more, 80% or more, 90% or more, 95% or more, 98% or more, 99% or more of the initial virus amount, preferably It means 100% retention.
  • the present invention also relates to the use of hydrophilic coating membranes to reduce the attachment of viruses to instruments.
  • the meaning of each term is as described above.
  • virus detection methods can be broadly classified into four types. The first is a method of detecting nucleic acids possessed by viruses, the second is a method of detecting proteins possessed by viruses, the third is a method of using the properties of viruses, and the fourth is a method of detecting virus particles themselves. is.
  • the method of detecting the nucleic acid possessed by the virus it is possible to identify and quantify the virus by detecting the sequence of the nucleic acid specific to the virus.
  • Hybridization methods Southern blot hybridization methods, Northern blot hybridization methods, in situ hybridization methods, microarray methods, and the like are applicable.
  • viruses can be identified and quantified by detecting virus-specific proteins. Examples include immunoassay and radioimmunoassay.
  • Viruses can be quantified by detecting their characteristic phenotypes (cytopathic effect, hemagglutination, etc.), and TCID50, PFU, HA, Such as LD50 is applicable.
  • viruses can be identified and quantified by detecting a single virus particle on the order of tens to hundreds of nanometers, and electron microscope observation (TEM, AFM, cryo-EM etc.), nanoparticle tracking analysis (NTA (NanoSight, Zeta View, etc.)), nanopore current measurement (qNano, etc.).
  • a specific detection device for example, a virus test kit
  • the lower limit of detection can be further lowered, and the performance of virus detection can be improved.
  • the coating of the present invention may be applied to the specimen-adhering portion of an extraction vessel used in Mizuho Medico's class III immunoassay series, adenovirus kit; Quick Chaser (registered trademark) Adeno, and the like.
  • primers used for PCR are known, for example, COG1F base sequence [5'-3'] CGY TGG ATG CGN TTY CAT GA (SEQ ID NO: 1), and are used for detection of the virus. It is preferably used for
  • Norovirus is preferably used as an antigen when viral protein 1 detects a virus using an antibody.
  • the virus specimen collection kit of the present invention comprises a combination of a tip attached to a pipette or the like, a virus extraction container, and a virus storage container, which is one embodiment of the above equipment, for collecting specimens.
  • ⁇ Method for reducing detection limit of virus test> When a virus test is performed using the device of the present invention, specifically using a virus test kit, the amount of virus adhering to the device is suppressed, so that a trace amount of virus present in the specimen can be detected. Lower limit of detection can be reduced. For example, detection of norovirus from sewage by environmental water (sewage) survey Usefulness of sewage norovirus monitoring for the purpose of early detection of infectious gastroenteritis epidemics (JSCE Journal G (Environment), Vol.72, No.
  • the present invention also relates to the use of hydrophilic coating membranes to reduce the detection limit of viruses.
  • the meaning of each term is as described above.
  • the device of the present invention is also a device for maintaining virus infectivity.
  • the infectivity of the virus is maintained, so that the virus can be appropriately preserved, studied, and evaluated.
  • "maintenance of infectivity” means that the virus infectivity is maintained, and the infectivity is determined by a method known to those skilled in the art, for example, the method described in Example 1 below. can be evaluated by
  • the present invention also relates to the use of hydrophilic coating membranes to retain the infectivity of viruses.
  • the meaning of each term is as described above.
  • choline 48-50% aqueous solution: manufactured by Japan Finechem Co., Ltd.
  • methacryloylcholine chloride 243 g of an 80% aqueous solution, manufactured by Mitsubishi Chemical Corp.
  • butyl methacrylate manufactured by Mitsubishi Chemical Corp.
  • Feline calicivirus adhesion evaluation test (a) Preparation of virus solution CRFK cells (manufactured by Dainippon Pharmaceutical Co., Ltd.) were infected with feline calicivirus (Feline calicivirus F-9 ATCC VR-782), cultured, and then centrifuged to remove cell debris. was removed and used as the virus solution.
  • TCID 50 tissue Culture Infectious Dose 50 measurement
  • three types of tubes were used: coated PP tubes, uncoated PP tubes and glass tubes.
  • a 10-fold serial dilution series (10 steps from ⁇ 10 1 to ⁇ 10 10 ) was prepared using a dilution medium (Eagle MEM medium "Nissui”) using the virus solution prepared in 3 types of tubes as the stock solution, and incubated at room temperature. Stored for 6 hours.
  • TCID 50 was calculated using the Reed-Muench method from the number of wells in which cytopathic effect (CPE) was observed at each virus dilution concentration, and the virus infectivity titer was measured. The results are shown in Table 1.
  • the PP tube coated with the coating film-forming composition of Preparation Example 1 maintains a virus concentration 10 ⁇ (6.93-6.67) ⁇ 1.8 times that of the uncoated PP tube by suppressing adhesion.
  • virus concentration 10 ⁇ (6.93-6.63) ⁇ 2.0 times compared to glass tube was maintained by adhesion suppression. Therefore, it was shown that the tube coated with the coating film-forming composition of Preparation Example 1 has the effect of suppressing virus adhesion even in the evaluation using TCID 50 as an index. It was also shown that the virus whose adhesion was suppressed maintained the state of infectivity.
  • a device with suppressed virus adhesion and a method for reducing virus adhesion using the device.
  • a virus storage container with less loss and a virus test kit with improved virus detection sensitivity can be provided.

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JP2008500104A (ja) * 2004-05-27 2008-01-10 アドヴァンスド カーディオヴァスキュラー システムズ， インコーポレイテッド ステント様インプラントにコートされたヘパリンを含有するブロックコポリマー
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JP2008500104A (ja) * 2004-05-27 2008-01-10 アドヴァンスド カーディオヴァスキュラー システムズ， インコーポレイテッド ステント様インプラントにコートされたヘパリンを含有するブロックコポリマー
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