WO2020037671A1 - 血样分析仪、血样分析方法及计算机存储介质 - Google Patents

血样分析仪、血样分析方法及计算机存储介质 Download PDF

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Publication number
WO2020037671A1
WO2020037671A1 PCT/CN2018/102313 CN2018102313W WO2020037671A1 WO 2020037671 A1 WO2020037671 A1 WO 2020037671A1 CN 2018102313 W CN2018102313 W CN 2018102313W WO 2020037671 A1 WO2020037671 A1 WO 2020037671A1
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Prior art keywords
sample
blood
container
mixing
blood sample
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Ceased
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PCT/CN2018/102313
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English (en)
French (fr)
Inventor
谢子贤
胡力坚
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Shenzhen Mindray Bio Medical Electronics Co Ltd
Shenzhen Mindray Scientific Co Ltd
Original Assignee
Shenzhen Mindray Bio Medical Electronics Co Ltd
Shenzhen Mindray Scientific Co Ltd
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Application filed by Shenzhen Mindray Bio Medical Electronics Co Ltd, Shenzhen Mindray Scientific Co Ltd filed Critical Shenzhen Mindray Bio Medical Electronics Co Ltd
Priority to PCT/CN2018/102313 priority Critical patent/WO2020037671A1/zh
Priority to CN201880096510.XA priority patent/CN112585445B/zh
Publication of WO2020037671A1 publication Critical patent/WO2020037671A1/zh
Priority to US17/170,862 priority patent/US20210223276A1/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/02—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations
    • G01N35/04—Details of the conveyor system
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F27/00—Mixers with rotary stirring devices in fixed receptacles; Kneaders
    • B01F27/80—Mixers with rotary stirring devices in fixed receptacles; Kneaders with stirrers rotating about a substantially vertical axis
    • B01F27/805—Mixers with rotary stirring devices in fixed receptacles; Kneaders with stirrers rotating about a substantially vertical axis wherein the stirrers or the receptacles are moved in order to bring them into operative position; Means for fixing the receptacle
    • B01F27/806—Mixers with rotary stirring devices in fixed receptacles; Kneaders with stirrers rotating about a substantially vertical axis wherein the stirrers or the receptacles are moved in order to bring them into operative position; Means for fixing the receptacle with vertical displacement of the stirrer, e.g. in combination with means for pivoting the stirrer about a vertical axis in order to co-operate with different receptacles
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F31/00—Mixers with shaking, oscillating, or vibrating mechanisms
    • B01F31/20—Mixing the contents of independent containers, e.g. test tubes
    • B01F31/201—Holders therefor
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F31/00—Mixers with shaking, oscillating, or vibrating mechanisms
    • B01F31/20—Mixing the contents of independent containers, e.g. test tubes
    • B01F31/23—Mixing the contents of independent containers, e.g. test tubes by pivoting the containers about an axis
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F31/00—Mixers with shaking, oscillating, or vibrating mechanisms
    • B01F31/44—Mixers with shaking, oscillating, or vibrating mechanisms with stirrers performing an oscillatory, vibratory or shaking movement
    • B01F31/441—Mixers with shaking, oscillating, or vibrating mechanisms with stirrers performing an oscillatory, vibratory or shaking movement performing a rectilinear reciprocating movement
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F33/00—Other mixers; Mixing plants; Combinations of mixers
    • B01F33/80—Mixing plants; Combinations of mixers
    • B01F33/82—Combinations of dissimilar mixers
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F35/00—Accessories for mixers; Auxiliary operations or auxiliary devices; Parts or details of general application
    • B01F35/10—Maintenance of mixers
    • B01F35/145—Washing or cleaning mixers not provided for in other groups in this subclass; Inhibiting build-up of material on machine parts using other means
    • B01F35/1452—Washing or cleaning mixers not provided for in other groups in this subclass; Inhibiting build-up of material on machine parts using other means using fluids
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/00584—Control arrangements for automatic analysers
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N2035/00465—Separating and mixing arrangements
    • G01N2035/00524—Mixing by agitating sample carrier
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N2035/00465—Separating and mixing arrangements
    • G01N2035/00534—Mixing by a special element, e.g. stirrer
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/02—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations
    • G01N35/04—Details of the conveyor system
    • G01N2035/0401—Sample carriers, cuvettes or reaction vessels
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/02—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations
    • G01N35/04—Details of the conveyor system
    • G01N2035/0401—Sample carriers, cuvettes or reaction vessels
    • G01N2035/0406—Individual bottles or tubes
    • G01N2035/041—Individual bottles or tubes lifting items out of a rack for access
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/02—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations
    • G01N35/04—Details of the conveyor system
    • G01N2035/0401—Sample carriers, cuvettes or reaction vessels
    • G01N2035/0412—Block or rack elements with a single row of samples

Definitions

  • the present application relates to the field of sample analysis, and in particular, to a blood sample analyzer and a blood sample mixing method for mixing and analyzing extracted micro-samples.
  • the analysis device In the clinical diagnosis process, it is often necessary to use an analysis device to measure blood, urine, body fluid (ascites, cerebrospinal, pleural fluid, etc.) samples collected from a patient.
  • the analysis device usually specifies the required sample size in advance.
  • blood samples there are currently two blood collection methods: venous blood and peripheral blood.
  • the venous blood collection method has a large blood volume ( ⁇ 1mL), which is usually suitable for adult patients. For infants, children, or severe patients, it is sometimes difficult to collect blood through the venous method. In this case, peripheral blood is often collected.
  • the volume of blood collected is small (mostly ⁇ 100 ⁇ L).
  • Blood collection tubes containing anticoagulants are usually used during blood collection.
  • Blood is composed of blood cells and plasma. Due to the different specific gravity of blood cells and blood samples, anticoagulated blood will stratify after standing for a period of time, so the blood samples must be thoroughly mixed before measurement, otherwise the measurement results will have a large deviation.
  • CN1334453A discloses a device for processing a blood product sample.
  • the device has a shaking device for stirring blood samples in a test tube.
  • the shaking device uses a clamping component to hold the test tube, and rotates the clamping component to make the test tube continuous at 360 °. Rotate to invert the test tube continuously up and down to stir the blood sample in the test tube upside down, thereby stirring the blood sample in the test tube.
  • venous blood samples For constant blood samples (venous blood samples), due to the large blood collection volume and good blood flowability, when the blood collection tube is inverted, venous blood will inevitably flow along the tube wall under the action of gravity.
  • the multiple inversion method disclosed in CN1334453A can be used Make the blood flow back and forth along the tube wall to achieve mixing.
  • CN1334453A the same stirring operation is used for constant blood samples and trace blood samples.
  • using the upside-down mixing method disclosed in CN1334453A will cause some blood to remain on the blood collection cap and tube wall and cause blood sample loss.
  • the lost blood sample accounts for a large proportion of the total blood collection volume. Small, does not affect the measurement.
  • the peripheral blood sample because the peripheral blood sample takes less blood and has poor fluidity, the peripheral blood tends to stick to the cap of the blood collection tube, the bottom of the blood collection tube, or the wall of the tube when the blood collection tube is reversed.
  • the upside-down mixing technology disclosed in the prior art will cause blood sample loss, adversely affect the measurement, and there is still difficulty in effectively solving the peripheral blood mixing problem. Therefore, using the upside-down mixing method disclosed in CN1334453A, even if a constant blood sample can be sufficiently stirred and homogenized, a trace amount of blood sample may not be stirred well.
  • CN103675309A discloses a sample processing device.
  • the processing device has a stirring motor part and a hand part.
  • the hand motor part is driven to rotate by the stirring motor part, so that the sample container is in an inverted state and upright. Spin between states.
  • the time for stirring the sample in the trace blood sample mode is longer than the time for stirring the sample in the constant blood sample mode, so that the trace blood The sample can be thoroughly stirred.
  • the time for stirring the sample in the micro blood sample mode is longer than the time for stirring the sample in the constant blood sample mode, so that the micro blood sample can be sufficiently stirred.
  • trace blood samples tend to stick to the cap or upper part of the tube wall.
  • CN107121559A discloses a method for mixing a mixed liquid of a peripheral blood sample and a diluent.
  • a sampling needle is inserted into a mixed liquid centrifuge tube, and the mixed liquid is processed by a method of automatic suction and discharge of the sampling needle. Mixing operation.
  • a whole blood sample it is not a homogeneous liquid, but consists of plasma (usually about 55% by volume) and blood cells (usually about 45% by volume). Blood cells can be understood as tiny particles whose density is generally slightly larger than plasma. Therefore, if a whole blood sample is added with an anticoagulant (to prevent blood clotting) and left for a period of time, the blood sample will delaminate in the blood collection tube 92: plasma is in the upper layer and blood cells are in the lower layer (see FIG. 1). If the automatic inhalation and ejection method disclosed in CN107121559A is used to mix the whole sample blood, the sampling needle is in the plasma layer or the blood cell layer due to the stratification of the blood sample. It is carried out in one of the plasma layer or blood cell layer. It is difficult to fully mix the plasma layer and the blood cell layer, and the amount of inhalation and discharge of the sampling needle each time is small, and it takes a long time to mix operating.
  • the present application proposes a sample analyzer and sample mixing method.
  • the device and method can effectively implement Stir minute whole blood samples such as peripheral blood samples.
  • a blood sample analyzer comprising: a sample transporting device for transporting a sample rack containing a first and / or a second sample container; a first mixing device having a means for stirring A sample stirring component for a blood sample in a first sample container, and the first mixing device is capable of driving the sample stirring component to the first mixing position of the sample rack containing the trace blood sample.
  • the blood sample in a sample container is mixed;
  • the second mixing device can obtain the sample rack or the second sample container on the sample rack, and can drive the A second sample container for a constant blood sample for mixing the blood samples;
  • a control device which is communicatively connected to the sample transport device, the first mixing device, and the second mixing device, and controls the sample transport device 2. The operations of the first mixing device and the second mixing device.
  • a blood sample analyzer which includes a sample chamber assembly having a sample chamber cover and a sample container fixing hole, and a unit for placing a micro blood sample or a constant blood sample on the sample container fixing hole.
  • Sample injection a mixing device having a sample stirring member for stirring the blood sample in the sample container, the mixing device can drive the sample stirring member to mix the blood sample in the sample container.
  • a blood sample analyzer including: a first mixing device capable of mixing blood samples in a first sample container; and a second mixing device capable of different from the above-mentioned
  • the method of the first mixing device mixes the blood samples in the second sample container;
  • the control device is communicatively connected with the first mixing device and the second mixing device, and can perform the following operations: (1) determine whether it is the first measurement mode or the second measurement mode; (2) when determining that it is the first measurement mode, control the first mixing device to mix the blood samples in the first sample container (3) When it is determined that the second measurement mode is performed, control the second mixing device to mix the blood samples in the second sample container.
  • a blood sample analyzer including: a sample transporting device for transporting a sample rack containing a sample container; a mixing device having a sample for agitating a blood sample in the sample container Stirring component, the mixing device can drive the sample stirring component to mix the blood sample in the sample container; a control device, which is communicatively connected with the sample transport device and the mixing device, and controls the sample transport The operation of the device and the mixing device.
  • a blood sample analysis method for blood routine including: transporting a sample container containing a blood sample to a mixing position; and driving a sample stirring component of a first mixing device to the sample.
  • the blood sample in the container is mixed; a predetermined sample amount of the blood sample is aspirated from the sample container on the mixing position to prepare a test sample for a routine blood test; a test for the test.
  • the relevant indexes of the blood routine detection item are obtained.
  • a fifth aspect of the present application provides a blood sample analysis method, including: a measurement mode determination step: determining whether a current measurement mode is a first measurement mode or a second measurement mode; a first test sample preparation step: In the first measurement mode, the first mixing device is controlled to drive the sample stirring unit to mix the blood samples in the sample container, and aspirate the first sampled blood sample to prepare the first test sample; the second test Sample preparation step: when it is judged that it is the second measurement mode, control the second mixing device to mix the blood sample in the sample container, and aspirate the blood sample of the second sample amount to prepare the second test A sample; and a detection step of detecting the first detection sample or the second detection sample.
  • a blood sample analyzer including: a sample transporting device for transporting a sample rack containing a sample container; a mixing device having a suction sample for sucking a blood sample in the sample container A device, the sample suction device can drive the sample suction of the sample suction device to suck and spit the blood sample in the sample container containing a trace amount of blood sample at the sampling position; a control device, the sample transportation device, The mixing device is communicatively connected to control the operations of the sample transport device and the mixing device.
  • a blood sample mixing method which includes: sucking a proper amount of air by a suction needle to form a section of isolated air column inside the suction needle; and driving the suction needle downward and close to a sample container. Bottom; after driving the aspirating needle to inhale an appropriate amount of blood sample, return the inhaled blood sample to the sample container, so that the blood sample in the sample container forms a certain flow until the The blood samples were mixed.
  • An eighth aspect of the present application provides a control device for a blood sample analyzer, including: at least one processor; and a memory storing instructions executable by the at least one processor, where the instructions are A processor, when executed, causes the blood sample analyzer to perform the method of any one of the above.
  • An eighth aspect of the present application provides a computer storage medium storing computer-executable instructions that, when executed by at least one processor of a blood sample analyzer, cause the blood sample analyzer to execute any of the above Item.
  • the sample transporting device can transport the sample rack containing the first and / or the second sample container to the first mixing position; the second mixing device can transfer the first mixing position from the first mixing position. Obtain the sample rack or the second sample container on the sample rack and transport it to the second mixing position.
  • the first mixing position is the same position as the second mixing position.
  • the head of the sample stirring member is cylindrical, paddle-shaped, or polygonal.
  • control device controls the sample stirring member to perform stirring in one or a combination of rotation, circular orbit, linear swing, or up and down oscillation modes.
  • sample stirring member can be moved up and down, and can be moved down to the first sample container on the first mixing position for stirring and mixing.
  • the blood sample analyzer further includes: a cleaning component for cleaning the sample agitating component; preferably, the cleaning component includes a cleaning liquid inlet and a cleaning liquid discharge outlet, for The sample stirring member in the component performs a cleaning operation; more preferably, the cleaning liquid discharge port can also be used for air extraction, so as to dry the sample stirring member.
  • the cleaning component includes a cleaning tank capable of cleaning the sample stirring component.
  • the blood sample analyzer further includes: a sample chamber assembly, including a sample chamber cover and a sample container fixing hole, for sampling a single sample of a micro blood sample or a constant blood sample placed on the sample container fixing hole.
  • a sample chamber assembly including a sample chamber cover and a sample container fixing hole, for sampling a single sample of a micro blood sample or a constant blood sample placed on the sample container fixing hole.
  • the blood sample analyzer further includes: the control device is further configured to determine whether the current sampling mode is the first sampling mode or the second sampling mode; when it is determined that the first sampling mode is the first sampling mode, controlling The sample transport device transports the sample rack containing the first and / or second sample container; when it is judged that the second sample mode is controlled, the sample chamber assembly is controlled to transport a single of the first and / Or a second sample container.
  • sample transport device transports the sample rack containing the first sample container to a predetermined position, and the grippers of the second mixing device can be grasped from the sample rack at the predetermined position.
  • the blood sample analyzer further includes: a measurement mode setting device for setting a first measurement mode and a second measurement mode; wherein the control device executes the following according to the settings of the measurement mode setting device Action: (1) determine whether it is the first measurement mode or the second measurement mode; (2) when it is determined that it is the first measurement mode, control the first mixing device to control the first sample The blood samples in the container are mixed; (3) when it is determined that the second measurement mode is the second mixing device, the second mixing device is controlled to grab the second sample container for mixing.
  • a measurement mode setting device for setting a first measurement mode and a second measurement mode
  • the control device executes the following according to the settings of the measurement mode setting device Action: (1) determine whether it is the first measurement mode or the second measurement mode; (2) when it is determined that it is the first measurement mode, control the first mixing device to control the first sample The blood samples in the container are mixed; (3) when it is determined that the second measurement mode is the second mixing device, the second mixing device is controlled to grab the second sample container for mixing.
  • the blood sample analyzer further includes: a sample suction device for sucking the mixed blood sample from the sample container; when it is determined that the first measurement mode is the first measurement mode, the control device controls the suction The sample device sucks a blood sample of a first blood collection amount from the first sample container; when it is determined that the second measurement mode is the control device, the control device controls the sample suction device from the second sample container Aspirate a blood sample of a second blood collection volume; wherein the first sampling volume is less than the second sampling volume; preferably, the first sampling volume is 5-50 ⁇ L, and more preferably 15-35 ⁇ L.
  • the measurement mode setting device is further configured to set a third measurement mode; when the control device determines that it is the third measurement mode, control the first mixing device to control the first sample container Mix pre-diluted blood samples.
  • the first mixing device mixes the trace whole blood sample of the first sample container; preferably, the trace whole blood sample of the first sample container is 30-250 ⁇ L, more preferably It is 50-200 ⁇ L, and more preferably 50-100 ⁇ L.
  • the repeated measurement of the hemoglobin value of the micro blood sample does not exceed ⁇ 2 g / L.
  • the blood sample analyzer is only used for processing trace whole blood samples and pre-diluted blood samples.
  • the blood sample analyzer further includes: a sample suction device for sucking the blood sample after mixing from the sample container; and a sample preparation device for suctioning the sample device
  • a blood sample is prepared as a test sample;
  • a control device is communicatively connected to the mixing device, the sample suction device and / or the sample preparation device, and controls the mixing device, the sample suction device and / or The operation of the sample preparation device.
  • the blood sample analyzer further includes: a measurement mode setting device for setting a first measurement mode and a second measurement mode; wherein the control device executes the following according to the settings of the measurement mode setting device Actions: (1) determine whether it is the first measurement mode or the second measurement mode; (2) when determining that it is the first measurement mode, control the sample suction device to remove the sample from the sample rack Aspirate a first sample of the blood sample in the container, and control the sample preparation device to prepare a first test sample; (3) when it is determined that the second measurement mode, control the sample suction device from Aspirate a second sample amount of the blood sample from the sample container on the sample rack, and control the sample preparation device to prepare a second test sample; wherein the first sample amount is less than the first sample amount.
  • Two sampling amounts preferably, the first sampling amount is 5-50 ⁇ L, and more preferably 15-35 ⁇ L.
  • the measurement mode setting device is further configured to set a third measurement mode; when the control device determines that it is the third measurement mode, control the blood analyzer to pre-dilute the blood sample, and then control the blood sample.
  • the sample suction device sucks a third sample amount of the pre-diluted blood sample from the sample container on the sample rack, and controls the sample preparation device to prepare a third detection sample.
  • control device can also perform the following operations: (1) determine whether it is the third measurement mode; (2) when the control device determines that it is the third measurement mode, control the first mixing device Mix the pre-diluted blood sample in the sample container.
  • the blood sample is a whole blood sample.
  • the blood sample analyzer further includes: a sample compartment assembly having a sample compartment cover and a sample container fixing hole for single sample injection of a blood sample placed on the sample container fixing hole; and / or A sample device for transporting a sample rack containing the first sample container and / or the second sample container.
  • control device can also perform the following operations: (1) determine whether it is the first sample mode or the second sample mode; (2) control the sample transport device when it is determined that it is the first sample mode Transporting the sample rack containing the sample container; (3) when it is determined that the second sample mode is in control, controlling the sample bin assembly to transport a single of the sample container to the blood analyzer.
  • the blood sample analysis method further includes: judging whether the current measurement mode is the first measurement mode or the second measurement mode; when it is determined that the first measurement mode is the first sampling mode, aspirating the first sample from the first sample container A sample of the mixed blood sample is prepared, and a first test sample is prepared.
  • the second measurement mode is, a second sampled sample of the mixed blood sample is pipetted from the second sample container, and the first Two test samples; wherein the first sampling amount is less than the second sampling amount; preferably, the first sampling amount is 5-50 ⁇ L, and more preferably 15-35 ⁇ L.
  • the blood sample analysis method further includes: determining whether the current measurement mode is the third measurement mode; and when determining that the current measurement mode is the third measurement mode, pipetting a third sample amount from the first sample container for mixing After the pre-diluted blood sample, a third test sample is prepared.
  • the blood sample analysis method further includes: judging whether the current sampling mode is the first sampling mode or the second sampling mode; when it is judged that the first sampling mode is, the sample is transported by the sample transport device. A container; when it is judged that it is the second sample injection mode, a single said sample container is transported by the sample compartment assembly to the blood analyzer.
  • the blood sample analysis method further includes: the second mixing device obtains the second sample container and mixes it upside down.
  • the measuring mode determining step it is further determined whether the current measuring mode is a third measuring mode; a third detection sample preparation step: when it is determined that the third measuring mode is, controlling the first measuring mode A mixing device mixes the pre-diluted blood sample in the sample container, and aspirates a third sample amount of the pre-diluted blood sample to prepare a third test sample; in the detecting step, detecting The third detection sample.
  • the blood sample analysis method further includes: a sampling mode determining step: determining whether the current sampling mode is a first sampling mode or a second sampling mode; a sample rack transporting step: when it is determined that the first sampling mode is the first sampling mode In the mode, the sample transport device is controlled to transport the sample rack containing the sample container to a predetermined position, and the mixed sample container to the first sampling position; the step of closing the sample compartment assembly: when it is judged that the In the second injection mode, the sample compartment assembly is closed, and the sample container is sent to the second sampling position.
  • the second mixing device transfers the sample container on the sample rack transported by the sample transport device to a predetermined position to the first mixing position for mixing; in the second detection In the sample preparation step, the second mixing device grabs a sample rack transported to a predetermined position by the sample transport device or the sample container on the sample rack and mixes it upside down.
  • sample suction device further comprises a suction suction device for driving the sample suction needle to suck and suck the blood sample in the sample container for mixing.
  • the suction driving device is a syringe.
  • the blood analyzer includes a mixing device capable of inverting and mixing a constant blood sample.
  • the suction and suction driving device can drive the sampling needle to suck an appropriate amount of air before mixing the blood samples in the sample container, so that a segment of isolated air column is formed inside the sampling needle.
  • sample suction device further comprises a sample suction air-drying device for air-drying the outer wall of the sample needle.
  • the sample suction device further includes a sample needle position sensor for sensing a downward position of the sample needle.
  • the blood sample mixing method further includes: determining whether the sampling mode is the first sampling mode or the second sampling mode; if the sampling mode is the first sampling mode, the sample transport device places the The sample container is transported to a first sampling position; if it is the second sampling mode, a single sample container is transported to a second sampling position by a sample bin assembly.
  • the method for mixing blood samples further comprises: air-drying the outer wall of the sampling needle before the sampling needle sucks an appropriate amount of air.
  • the device and method disclosed based on the above technical solution can effectively achieve the uniform stirring of trace samples such as peripheral blood samples, and can simultaneously solve the loss of trace blood samples such as peripheral blood caused by the blood sample remaining on the rubber cap and causing the analyzer to suck.
  • Technical problems that affect the measurement results due to insufficient samples can also enable the analytical instrument to be miniaturized while solving existing technical problems.
  • FIG. 1 is a schematic diagram showing the stratification of blood samples in blood collection tubes
  • FIG. 2 is an external perspective view of a blood sample analyzer according to an embodiment of the present application
  • FIG. 3 and 4 are schematic structural diagrams of a sample transport device according to an embodiment of the present application.
  • FIG. 5 is a schematic structural diagram of a first mixing device according to an embodiment of the present application.
  • FIG. 6 is a schematic structural diagram of a second mixing device according to an embodiment of the present application.
  • FIG. 7 is a schematic structural diagram of a sample suction device according to an embodiment of the present application.
  • FIGS. 8 to 11 are schematic structural diagrams of a container rotary scanning device according to an embodiment of the present application.
  • FIGS. 12 to 13 are schematic diagrams of working principles of a container rotary scanning device according to an embodiment of the present application.
  • 15 is a schematic diagram of a sample rack when a sample container is installed in the present application.
  • FIG. 16 is a schematic diagram of a sample holder when a micro blood collection tube is installed in the present application.
  • FIG. 17 is a schematic structural diagram of an adapter according to the present application.
  • FIG. 18 is a schematic structural diagram of another adapter according to the present application.
  • 19 is a structural block diagram of a control device of the present application.
  • FIG. 20 and FIG. 21 are block diagrams of the main flow of an example of processing and analyzing a blood sample by the blood sample analyzer of the present application;
  • 22 is a schematic diagram of a setting interface of a blood analyzer of the present application.
  • FIG. 23 is an example of a mixing operation of the first mixing device in an embodiment of the present application.
  • FIG. 24 is a schematic block diagram of a mixing operation in step S11 in an embodiment of the present application.
  • FIG. 25 is a cleaning example diagram of a stirring member of a first mixing device in an embodiment of the present application.
  • FIG. 26 is a diagram illustrating an example of aspirating a sample in step S13 in a first injection mode in an embodiment of the present application.
  • FIG. 27 is a diagram illustrating an example of aspirating a sample in step S22 in a second injection mode according to an embodiment of the present application.
  • FIG. 28 and FIG. 29 are block diagrams of the main flow of another example of processing and analyzing a blood sample by the blood sample analyzer of the present application;
  • FIG. 30 is a main flow block diagram of another example of processing and analyzing a blood sample by the blood sample analyzer of the present application.
  • FIG. 31 is a diagram showing analysis data of 100 ⁇ L of micro whole blood blood samples
  • FIG. 32 is a data diagram of 6 microwhole blood samples of different volumes each being mixed with the first mixing device 11 of the first embodiment of the present application, and the HGB is detected in the first measurement mode;
  • FIG. 33 is a schematic structural diagram of a mixing device in an embodiment of the present application.
  • FIG. 34 is a schematic structural diagram of another sample suction device according to an embodiment of the present application.
  • the peripheral blood sample has a small amount of blood and poor fluidity
  • the peripheral blood is often stuck to the bottom of the blood collection tube or the wall of the tube when the blood collection tube is inverted, and the blood sample is spilled or lost due to upside-down mixing.
  • Traditional upside-down mixing technology It is difficult to solve the problem of peripheral blood mixing.
  • the present application proposes a method for automatically mixing micro-samples, a mixing device, and an analyzer with a function for automatically mixing micro-samples, which realize the mixing of micro-samples by driving a stirring component to move in a sample container. .
  • FIG. 2 is an external perspective view of the blood sample analyzer according to the present embodiment.
  • the blood sample analyzer 1 includes an instrument main body, a casing 30, a sample transport device 17 disposed in front of the instrument main body, and the like.
  • the housing 30 is provided with a display part 31, operation buttons 32, and operation buttons 33.
  • the display part 31 can be a touch screen that can be operated by touch. By touching the display part 31, an input device 23 (such as a soft keyboard) can be displayed. (Refer to Figure 19).
  • the input device 23 may also be provided independently as hardware.
  • the above-mentioned instrument main body can be basically accommodated in the casing 30, and includes a first mixing device 11 for mixing blood samples in a sample container (blood collection tube) 91 and a second mixing device 12 for mixing blood samples in a sample container (blood collection tube) 92. 2.
  • Aspirating device 13 aspirating the blood sample mixed by the first mixing device 11 or the second mixing device 12 from the sample container 91 (92), and taking the sample transporting device 17 and transporting it to the scanning position (not shown)
  • Container rotary scanning device including container pressing component 14, container rotating component 15, code scanner 16
  • Sample preparation device for blood sample preparation and detection
  • a detector for detecting blood cells in blood from the detection sample prepared by the sample preparation
  • display unit 31 operation button 32
  • the control device 21 which is electrically connected to the operation button 33 and the corresponding part of the instrument body.
  • the blood sample analyzer 1 may further include a sample chamber assembly 18, which is used for the single sample injection of a trace whole blood sample or a constant blood sample, and is generally used for the measurement of emergency cut-off samples.
  • the sample chamber assembly 18 has a sample chamber cover 181 and a sample container fixing hole 182.
  • the sample compartment cover 181 can be opened to place the sample container containing the emergency cut-out sample into the sample container fixing hole 182 to fix the sample container, or remove the sample container from the sample container fixing hole 182 .
  • the diameter of the sample container fixing hole 182 is slightly larger than the outer diameter of the sample container or adapter 81 (82) (see FIGS. 17 and 18) that needs to be placed.
  • the sample transporting device 17 includes a sample rack supporting member 171, a sample rack feeding device 172, a sample rack bidirectional sample transporting device 173, and a sample rack feeding device 174.
  • the sample rack supporting member 171 includes a pre-analytical sample rack storage area 1711 where a plurality of sample racks 80 holding sample containers containing pre-analyzed samples can be placed, and a sample rack 80 which can hold several sample containers containing post-analytical samples.
  • the post-analysis sample rack storage area 1712 and the sample analysis area located between the pre-analysis sample rack storage area 1711 and the post-analysis sample rack storage area 1712.
  • a sample rack is sent into the turning area 1711a, and after the analysis, the sample rack storage area 1712 is provided with a sample rack out of the turning area 1712a.
  • the sample rack feeding device 172 has sample rack feeding parts 1721 and 1722.
  • the sample rack feeding parts 1721 and 1722 can move the sample rack 80 stored in the sample rack storage area 1711 before analysis to the sample rack by moving in the Y2 direction. Enter the steering zone 1711a.
  • the sample rack feeding parts 1721 and 1722 are driven by a stepping motor (not shown).
  • the sample rack 80 that enters the sample rack and enters the turning area 1711a will continue to be transported in the X1 direction by the sample rack bidirectional sample transport device 173.
  • the sample container 91 (92) containing the sample entering the analysis area will be sequentially transported to the scanning position and scanned by the container rotary scanning device 14-16, and then sent to a predetermined position by the first mixing device 11 to the sample container 91.
  • the sample rack 80 to which the sample container containing the sample is fixed is transported by the sample rack bidirectional sample transport device 173 to the sample rack delivery turning area 1712a, and the sample rack sending part 1741 of the sample rack sending device 174 will move horizontally in the direction of Y1 to move the sample.
  • the rack 80 is moved to the post-analysis sample rack storage area 1712.
  • the sample rack sending part 1741 is driven by a stepping motor (not shown).
  • FIG. 5 is a schematic structural diagram of a first mixing device according to this embodiment.
  • the first mixing device 11 can be movably mounted on the casing 30 or other support (not shown) of the blood sample analyzer 1, and can be moved up, down, left and right, or rotated by a motor drive.
  • the first mixing device 11 includes a stirring member driving motor 111, a sample stirring member 112, and a cleaning member 113.
  • the stirring component motor 111 may be a stepping motor, a servo motor, or a DC motor. In this embodiment, preferably, the motor 111 is a stepping motor.
  • the sample stirring member 112 may be a stirring rod having a cylindrical shape, a paddle shape, a polygonal shape, or the like, and driven by the stirring member motor 111 in one or more of rotation, circular orbit, linear swing, or up and down vibration modes. The combination of these methods performs the stirring action. At the same time, the sample stirring member 112 can drive the mixing device 11 to move up and down, and to the left and right under the driving of a driving device (not shown) of the mixing device.
  • the cleaning member 113 includes a cleaning liquid inlet 1131 and a cleaning liquid discharge port 1132.
  • the cleaning unit 113 performs the cleaning operation on the sample stirring unit 112 located therein to prevent the blood samples in the next blood sample containers from being stirred.
  • the cleaning solution is input through the cleaning solution inlet 1131 to clean the sample stirring member 112, and the cleaning solution after the cleaning is discharged through the cleaning solution.
  • the outlet 1132 is discharged, and the cleaning liquid is recovered.
  • the sample stirring member 112 may be dried, for example, the sample stirring member 112 is evacuated through the cleaning liquid discharge port 1132 to air dry the sample stirring member 112. .
  • the cleaning liquid discharge port 1132 is directly used for air extraction and air drying, which can save air drying equipment and make the device compact.
  • the sample stirring unit 112 of the first mixing device 11 is driven to perform the mixing operation on the samples in the sample container, so that the components of each layer in the whole blood sample in the sample container 91 can be quickly mixed. Moreover, during the mixing process, the bottom of the sample container 91 does not exceed the nozzle, and the sampling needle of the analyzer 1 does not contact the blood sample in the sample container 91, and the mixing device 11 can prevent the blood sample from being spilled and the blood sample not sticking to the tube. Mix the micro samples under the premise of the cap.
  • the sealed lid of the sample container 91 needs to be opened, or the sample container 91 without the sealed lid is used.
  • the sample container 91 contains a trace blood sample, usually 50 ⁇ L or more and 250 ⁇ L or less, such as 100 ⁇ L; the sample contained in the sample container may be a whole blood sample or a pre-diluted sample; the The sample contained in the sample container 91 may be a small amount of peripheral blood or a small amount of venous blood. As long as the volume is less than or equal to 250 ⁇ l, it is suitable for mixing with the first mixing device 11, and is particularly suitable for a volume greater than or equal to 50 ⁇ L and less than Mixing of micro whole blood samples equal to or less than 200 ⁇ L.
  • FIG. 6 is a schematic structural diagram of a second mixing device according to this embodiment.
  • the second mixing device 12 can grab the constant blood sample container sent by the sample transport device 17 placed on the sample rack 80 to a predetermined position of the analyzer 1 and mix the samples in an upside down mixing manner.
  • the constant blood sample may be a venous blood collection tube 92 or another type of venous blood collection tube; the constant blood sample container contains a second sample volume (constant) that is significantly larger than the first sample volume (trace blood sample) Blood sample), the second sample volume is usually ⁇ 1 mL; the sample contained in the constant blood sample container is a venous whole blood sample.
  • the second mixing device 12 includes: a gripper 1201, a first support frame 1211, a second support frame 1212, a third support frame 1213, a stepper motor 1221 to 1223, and a linear slide 1231 to 1232.
  • the first support frame 1211 is the main support of the second mixing device 12 and is used to fix the stepping motor 1221, the linear slide 1231 and the position sensors 1251 to 1252, and the first support frame 1211 is fixed to the analyzer 1 by screws.
  • Front plate the linear slide rail 1231 is placed in the Z1 and Z2 directions, and the second support frame 1212 and the sensor sensing piece 1261 are connected to the slider of the linear slide rail 1231 and can slide in the Z1 or Z2 direction
  • the second support frame is used for fixed steps
  • the linear slide 1232 is placed in the Y1 and Y2 directions, and the third support frame 1213 and the sensor induction piece 1262 are connected to the slider of the linear slide 1232, and can be along Y1 Or slide in Y2 direction;
  • the third support frame 1213 is used to fix the stepper motor 1223, the position sensor 1255, and the rotating shaft 1271 is fixed on the third supporting frame 12
  • the endless synchronous toothed belt 1241 is driven by the rotation of the stepping motor 1221 and rotates under the guidance of two synchronous wheels.
  • the second support frame 1212 is connected to the endless synchronous toothed belt 1241. Driven by the stepping motor 1221, the second support frame 1212 can drive the gripper 1201 and the sensor induction piece 1261 to move in the Z1 or Z2 direction; the position sensors 1251 and 1252, With the sensor induction piece 1261, it is used to realize the positioning of the gripper 1201 in the Z1 or Z2 direction.
  • the position sensor 1252 is used for positioning.
  • position sensor 1251 is used for positioning.
  • the endless synchronous toothed belt 1242 is driven by the rotation of the stepping motor 1222 and rotates under the guidance of two synchronous wheels.
  • the third support frame 1213 is connected to the endless synchronous toothed belt 1242. Driven by the stepping motor 1222, the third support frame 1213 can drive the gripper 1201 and the sensor induction piece 1262 to move in the Y1 or Y2 direction; the position sensors 1253 and 1254, With the sensor induction piece 1262, it is used to realize the positioning of the gripper 1201 in the Y1 or Y2 direction.
  • the third support frame 1213 drives the gripper 1201 to move along Y1
  • the position sensor 1254 is used for positioning.
  • position sensor 1253 is used for positioning.
  • the endless synchronous toothed belt 1243 is driven by the rotation of the stepping motor 1223 and rotates under the guidance of two synchronous wheels.
  • the rotating shaft 1271 is driven by the stepping motor 1223 to drive the gripper 1201 to rotate in the R7 or R8 direction; the position sensor 1255 cooperates with the sensor induction piece 1263 to realize the positioning of the gripper when the gripper moves in the R8 direction.
  • FIG. 7 is a schematic structural diagram of a sample suction device according to this embodiment. As shown in FIG. 7, the sample suction device 13 is used to draw a blood sample from a sample container 91 (92) sent from the sample transport device 17 to the sampling position of the analyzer 1 for sample preparation.
  • the suction device 13 includes: a suction needle 135, a suction needle moving assembly 131, a stepper motor 1301, synchronous wheels 1302 and 1303, and an endless synchronous toothed belt 1304 wound on the synchronous wheels 1302 and 1303. , Linear guides 1305, position sensors 1306, etc. placed along the Y1 and Y2 directions.
  • the suction needle moving component 131 is connected to the endless synchronous toothed belt 1304 through a connecting member.
  • the endless synchronous toothed belt 1304 is driven by the rotation of the stepper motor 1301 and rotates under the guidance of two synchronous wheels 1302 and 1303.
  • the suction needle moving component 131 can drive the suction needle 135 to move in the Y1 or Y2 direction under the driving of the stepper motor 1301.
  • the initial position of the suction needle moving component 131 in the Y1 and Y2 directions is positioned by a position sensor 1306 and a sensor sensing piece 1318 fixed to the suction needle moving component 131.
  • the sample needle moving assembly 131 includes a stepper motor 1311, a screw 1312, a nut 1323, a linear slide rail 1314, a sample needle fixing member 1315, a position sensor 1316, and a sensor induction sheet 1317.
  • the aspiration needle 135 is fixed on the aspiration needle fixing part 1315, and the aspiration needle fixing part 1315 is fixed on the linear slide rail 1314 placed along Z1 and Z2 by screws, and at the same time, the nut 1313 is stuck on the aspiration needle fixing part 1315. In the slot, relative rotation between the nut 1313 phase and the suction needle fixing member 1315 does not occur.
  • the lead screw 1312 is connected to the rotating shaft of the stepping motor 1311 by screws.
  • the stepping motor 1311 can drive the screw 1312 to rotate, and drive the suction needle fixing member 1315 to move the suction needle 135 in the Z1 or Z2 direction.
  • the initial positions of the suction needles 135 in the Z1 and Z2 directions are positioned by a position sensor 1317 and a photocoupler sensor sensing piece (not shown) provided on the suction needle fixing member 1315.
  • the sample suction needle 135 can move in two dimensions in the Y1, Y2 directions and Z1, Z2 directions. It can realize the function of drawing blood samples from the sample container and dividing blood samples into the sample preparation device.
  • FIGS. 8 to 11 are schematic structural diagrams of a container rotary code scanning device according to this embodiment.
  • FIGS. 12 to 13 are schematic diagrams of the working principle of a container rotary code scanning device according to this embodiment.
  • the container rotation scanning device 14 to 16 (where 14 is a container pressing component, 15 is a container rotating component, and 16 is a code scanner) are used to obtain the sample container label sent by the sample transport device 17 to the scanning position of the analyzer 1 for reading.
  • the coded information of the sample book is used for the sample information management of the analyzer.
  • the container pressing assembly 14 includes a stepping motor 141, a driven wheel bracket 142, a linear slide rail 143, and two driven wheels 144 a and 144 b.
  • the driven wheels 144a and 144b are rotatably fixed to the driven wheel bracket 142, and the driven wheel bracket 142 is fixed to the slider of the linear slide rail 143.
  • the linear slide rail 143 is provided along the Y1 and Y2 directions.
  • the driven wheel bracket 142 can move the driven wheels 144a and 144b in the Y1 and Y2 directions.
  • the driven wheel bracket 142 is provided with a notch 1421 for avoiding the scanning window of the scanner 16.
  • the container rotation assembly 15 includes a stepping motor 151, a rotating wheel 152, a rubber pad 153, and a coupling 154.
  • the rotating wheel 152 is connected to the rotating shaft of the stepping motor 151 through a coupling 154. Under the driving of the stepping motor 151, the rotating wheel 152 can rotate counterclockwise or clockwise.
  • the rubber pad 153 is placed on the outer ring of the rotating wheel 152 to increase the friction between the rotating wheel 152 and the container.
  • the driven wheel bracket 142 can move the driven wheels 144 a and 144 b in the Y1 direction to squeeze the sample container toward the container rotating assembly 15 Rotating wheel 152 (see FIG. 11).
  • the sample container and the rollers 144a and 144b are respectively wound around their respective central axes O2, O3 and O4 rotates along R12, R13, and R14; or the stepper motor 151 drives the rotating wheel 152 to rotate around the center O1 point in the direction of R11 ', and the sample container and the rollers 144a and 144b respectively rotate around their respective central axes O2, O3, and O4 along R12' , R13 ', and R14' (see Fig. 12).
  • the barcode label affixed to the sample container will face the barcode reader 16 at one stage.
  • the scanner 16 can read the number information of the barcode label on the sample container (refer to Figure 13). .
  • the container rotation scanning device can support scanning of a sample container having a suitable height and an inner diameter, which can be put into a test tube rack, and a barcode can be affixed on the tube wall, preferably an elongated sample container.
  • FIG. 14 is a schematic structural diagram of a sample rack according to this embodiment.
  • the sample rack 80 is provided with fixing holes 801a for fixing the sample container, and each fixing hole 801a is correspondingly provided with an opening 801b, and the opening 801b serves as a scanning window for the barcode label of the sample container;
  • a sample rack label pasting area 802 is provided.
  • the sample rack label pasting area 802 can be used to attach labels such as barcode labels, two-dimensional code labels, or RFID labels.
  • the encoded information of the label of the sample rack 80 includes measurement mode information.
  • the sample holder 80 may directly fix a plurality of venous blood collection tubes 92 or micro blood collection tubes 91 (see FIG. 15), or may fix the micro blood collection tubes 91 through an adapter 81 (see FIG. 16).
  • the adapter 81 (see FIG. 17) is provided with a fixing hole 811 for fixing the micro blood collection tube 91, and a step 812, which can prevent the adapter 81 from falling during the ascent of the gripper 1201, and a bottom portion is provided to reduce the weight of the adapter. Cavity 814.
  • the adapter 81 has a cap restriction portion 813 that can block the micro blood collection tube 91 than the adapter 82. When the micro blood collection tube 91 is placed in the fixing hole 811 of the adapter 81, the connection portion 913 of the micro blood collection tube 91 needs to be clicked in. Restriction part 813 of the adapter 81.
  • the cap and the tube of the micro blood collection tube 91 are inseparable, in order to prevent the cap from being covered to the tube under the restoring force of the connection portion, the cap is restricted by the restriction portion 813 of the adapter 81 to prevent the sampling needle from entering the micro tube
  • the blood vessel 91 is stuck on the cap during aspiration.
  • the restriction portion 813 is provided in a zigzag shape.
  • the diameter of the outer wall of the adapter 81 is smaller than the diameter of the sample container fixing hole 801a of the sample holder 80.
  • the inner diameter of the fixing hole 811 of the adapter 81 is slightly larger than the outer diameter of the fixed micro blood collection tube 91 tube. equal.
  • the adapter 82 (see FIG. 18) is provided with a fixing hole 821 for fixing the micro blood collection tube 91, and a step 822 (the function is the same as the step 812 of the adapter 81).
  • the adapter 82 is provided with a cavity 823 at the bottom to reduce weight. .
  • the diameter of the outer wall of the adapter 82 is smaller than the diameter of the fixing hole 801a of the sample holder 80, and the inside diameter of the fixing hole 821 of the adapter 82 is slightly larger than the outside diameter of the tube of the fixed micro blood collection tube 91.
  • the above-mentioned micro blood collection tube 91 can collect less blood samples regardless of whether the peripheral blood is collected through a capillary tube or a blood scraping method (most cases ⁇ 100 ⁇ L, and in rare cases 200-250 ⁇ L).
  • the sample container 91 may be one or more kinds of micro blood collection tubes, or may be other types of micro tubes; the sample amount of the micro sample is usually ⁇ 250 ⁇ L, preferably 30 to 250 ⁇ L. , More preferably 50 to 200 ⁇ L, and even more preferably 50 to 100 ⁇ L; the trace sample may be a whole blood sample or a pre-diluted sample; the trace sample may be a trace amount of peripheral blood, or a trace amount Venous blood.
  • FIG. 19 is a block diagram of the configuration of the control device 21.
  • the control device 21 is mainly composed of a CPU 211a, a ROM 211b, a RAM 211c, a hard disk 211d, a reading device 211e, an input / output interface 211f, a communication interface 211g, and an image output interface 211h.
  • the CPU 211a, ROM211b, RAM211c, hard disk 211d, reading device 211e, input / output interface 211f, communication interface 211g, and image output interface 211h are connected via a bus 211i.
  • the CPU 211a can execute a computer program stored in the ROM 211b and a computer program downloaded to the RAM 211c.
  • the CPU 211a executes the applications 214a, 214b, and 214c described later, and thereby functions as the control device 21.
  • the ROM 211b is composed of a mask ROM, a PROM, an EPROM, an EEPROM, and the like, and stores therein a computer program executed by the CPU 211a and data required by the computer program.
  • the RAM 211c is composed of SRAM, DRAM, or the like.
  • the RAM 211c is used to read computer programs stored in the ROM 211b and the hard disk 211d.
  • the RAM 211c can also be used as a work space when the CPU 211a executes these computer programs.
  • the hard disk 211d is provided with various computer programs for execution by the CPU 211a, such as an operating system and application programs, and data used in executing the computer programs.
  • the first mixing processing program 214a for the first mixing device 11, the second mixing processing program 214b for the second mixing device 12, and the sample transporting processing program 214c for the sample transport device 17 are also installed on this hard disk. 211d.
  • the CPU 211a executes these application programs 214a to 214c, thereby controlling the operations of each part of the first mixing device 11, the second mixing device 12, and the sample transport device 17.
  • the reading device 211e includes a floppy disk drive, a CD-ROM drive, a DVD-ROM drive, and the like, and can read computer programs or data stored in the portable storage medium 214.
  • Application programs 214a to 214c are stored in the portable storage medium 214, and the control device 21 can read the application programs 214a to 214c from the portable storage medium 214, and can load these application programs 214a to 214c into the hard disk 211d.
  • the application programs 214a to 214c can be provided not only from the portable storage medium 214, but also from an external device that is communicably connected to the control device 21 via an electronic communication line (regardless of wired or wireless) via the electronic communication line. .
  • An operating system capable of providing a graphical user interface is installed in the hard disk 211d.
  • the application programs 214a to 214c all run on the above operating system.
  • the input / output interface 211f includes a serial interface, a parallel interface, and an analog interface including a D / A converter, an A / D converter, and the like.
  • the input / output interface 211f is connected to an input device 23, and the user can input data to the control device 21 using the input device 23.
  • the communication interface 211g is a wired or wireless communication interface.
  • the control device 21 can transmit data with the first mixing device 11, the second mixing device 12, and the sample transport device 17 using a certain communication protocol through the communication interface 211g.
  • the image output interface 211h is connected to a display section 31 composed of an LCD, a CRT, or the like, and outputs an image signal corresponding to the image data received from the CPU 211a to the display section 31.
  • the display unit 31 displays an image (interface) in accordance with the input video signal.
  • the control device 21 controls the operations of the first mixing device 11, the second mixing device 12, and the sample transport device 17 through the above-mentioned structure.
  • FIG. 20 and FIG. 21 are block diagrams of the main flow of an example of the blood sample analyzer 1 analyzing and processing a blood sample.
  • the power of the blood sample analyzer 1 is turned on, and the control device 21 starts initialization (step S1).
  • the initialization of the program and the initialization of the liquid circuit components of the blood sample analyzer 1, the cleaning of the pipeline, and the resetting of the driving part are performed.
  • step S2 the control device 21 determines whether or not it is necessary to set the sample rack type. If it is necessary to set the sample rack type (step S2: YES), it proceeds to step S3; if it is determined that there is no need to set the sample rack type (step S2: NO), it proceeds to step S5.
  • step S3 the display unit 31 displays a sample rack type setting interface (refer to FIG. 22), and the user enters the sample rack type setting interface to set the sample rack information.
  • the number of the sample rack 80 can be set in the first column of the interface, and the sample rack 80 with the corresponding number can be set as the micro whole blood sample rack or the pre-diluted blood sample rack. If the micro whole blood sample rack or pre-diluted blood sample rack is not checked, or the numbered sample rack 80 is not set on this interface, the blood sample analyzer 1 will be treated as a constant blood sample rack.
  • the sample container on the corresponding sample rack 80 and the blood sample analyzer 1 are all treated as the first sample container 91; the micro whole blood sample is not checked
  • the blood sample analyzer 1 treats the sample container on the rack or the pre-diluted blood sample rack, or the sample container on the sample rack 80 without a set number in this interface as the second sample container 92.
  • the aspiration needle 135 of the blood analyzer 1 Aspirates from the first sample container 91 on the sample rack 80 of the corresponding number.
  • the first sample volume of blood sample is, for example, preferably 5-50 ⁇ L, more preferably 15-35 ⁇ L, and the most commonly used is 30 ⁇ L; when the pre-dilution (third measurement mode) is checked in the second column, the suction of the blood analyzer 1 When the sample needle draws the sample from the first sample container 91 on the corresponding numbered sample rack 80, the third sample blood sample is sucked, for example, 80 ⁇ L; when the micro whole blood sample rack or the pre-diluted blood sample rack is not checked (Second measurement mode), when the sampling needle of the blood analyzer 1 sucks a sample from the second sample container 92 on the sample rack 80 of the corresponding number, a second sample amount of blood sample is aspirated, for example, 50-300 ⁇ L, The most commonly used 70
  • FIG. 22 is a setting interface of the blood analyzer 1.
  • the user can call the setting interface through the display part 31 and set certain numbered sample racks as sample racks for micro blood collection tubes.
  • the blood analyzer 1 will be treated as a sample rack dedicated to micro blood collection tubes.
  • the container 91 is transferred to the first mixing device 11 for mixing (explained in detail later).
  • the blood analyzer 1 will be treated as a normal venous blood collection tube sample rack.
  • the second mixing device 12 is used to grab the sample container 92 on the sample rack. Mix well.
  • the user can also set the type of the micro blood collection tube fixed by the micro blood collection sample holder (refer to the third column of Fig. 22).
  • the tube type is optional
  • the sample volume contained in the micro blood collection tube (refer to the fourth column of FIG. 21)
  • the control device 21 of the blood analyzer 1 automatically selects the first mixing device 11 for driving the sample according to the user's setting
  • the rotation speed of the motor 111 of the agitating part 112 among which the type, size of the micro blood collection tube, and the relationship between the sample volume and the rotation speed of the motor, have been set in advance in a software program.
  • the control device of blood analyzer 1 21 Set the rotation speed of the motor 111 of the first mixing device 11 for driving the sample stirring part 112 to a default rotation speed for mixing the sample containers on the numbered sample rack.
  • the blood analyzer 1 will use the first mixing device 11 to mix the sample containers on the sample rack, and to sample samples other than numbers 1 to 5
  • the analyzer 1 will use the second mixing device 12 to mix the sample containers on the sample rack.
  • the sample racks with numbers 1-5 can be further distinguished. Sample containers with different volumes of blood samples, or sample containers with different shapes or sizes can be placed on the sample racks with numbers 1-3, 4 and 5, respectively.
  • the motor 111 of the first mixing device 11 uses M1 revolutions per revolution for mixing; for the sample containers on the sample rack number 4, the first mixing is performed.
  • the motor 111 of the device 11 performs mixing at a default rotation speed of M0 revolutions / revolution; for the sample container on the sample rack numbered 5, the motor 111 of the first mixing device 11 uses the rotation speed of M2 revolutions / revolution to perform mixing.
  • the blood analyzer 1 treats all the sample containers on the sample rack 80 numbered 1 to 5 as the first sample container 91, and treats the sample containers on the sample racks numbered 1-5 as the second sample container. 94 processing.
  • the aspiration needle of blood analyzer 1 aspirates from the sample container 91 on the sample racks numbered 1 to 4, pipette the first sample amount of blood sample (micro whole blood sample); the aspiration needle of blood analyzer 1
  • the third sample volume of the blood sample pre-diluted blood sample
  • the aspiration needle of the blood analyzer 1 starts from samples with numbers other than 1-5.
  • a second sample amount of blood sample is pipetted.
  • step S5 the user selects an injection mode.
  • step S6 the control device 21 determines whether it is the first injection mode. If it is determined as the first injection mode (step S6: YES), the control device 21 determines whether or not a start button (not shown) is pressed (step S7). If the control device 21 determines that the start button has not been pressed (step S7: NO), it proceeds to step S25. If it is determined that the start button has been pressed (step S7: YES), the process proceeds to step S8.
  • step S8 the sample transport device 17 transports the sample containers 91 (92) on the sample rack 80 to the scanning position (not shown) one by one, and the container rotation scanning device (including the container pressing component 14 and the container rotating component 15) 16 ⁇ Code Scanner 16) Read the sample code information on the sample container 91 (92) label 911 (921), and scan the sample rack 80 that has passed the code scanning position, and read the code information of the sample rack 80 label (step S9).
  • the control device 21 controls the sample transport device 17 to transport the sample containers 91 (92) on the sample rack 80 to the predetermined position 22 one by one (step S10).
  • the control device 21 controls the first mixing device 11 or the second mixing device 12 to perform a mixing operation on the blood sample in the sample container 91 (92) (step S11).
  • the control device 21 is based on the slave sample rack 80
  • the encoded information read by the tag determines whether the current measurement mode is the first measurement mode, the second measurement mode, or the third measurement mode.
  • control device 21 determines that the current measurement mode is the first measurement mode or the third measurement mode, it moves the first
  • the mixing device 11 performs agitation and mixing operation on the blood samples in the sample container 91 on the sample rack 80 at the predetermined position 22 (first mixing position); if the control device 21 determines that the current measurement mode is the second measurement mode Control the gripper 1201 of the second mixing device 12 to grab the current sample container 92 from the sample rack 80 at the predetermined position to a certain position (second mixing position) (not shown), and control the second mixing
  • the stepping motor 1223 of the device 12 drives the gripper 1201 to rotate, thereby performing the mixing operation of the blood sample on the current sample container.
  • the first mixing position can be set on the sample rack, that is, the hole position on the sample rack is used as the first mixing position, and the stirring portion is moved to this position to the test tube in the first mixing position.
  • the samples are mixed; or the first mixing position is a fixed position separately set relative to the sample rack, which is convenient for better fixing the test tube during the stirring operation.
  • a carrying device may be additionally provided to grab the first sample container and transport it to the first mixing position, or a grasping mechanism of the second mixing device may be used as the holding position. The carrying device transports the first sample container.
  • the first mixing position can be set on the sample holder, preferably, as shown in FIG.
  • control device 21 controls the container pressing assembly 14 to move so that the two driven wheels 144 a and 144 b clamp the sample container on the sample holder 80. 91 (92), and controls the sample stirring member 112 of the first mixing device 11 to enter the blood sample in the sample container 91 (92) downward (Z direction) to perform the mixing operation.
  • the gripper 1201 of the second mixing device 12 may grab the sample container 91 (92) from the sample rack 80 at the predetermined position 22 and move it to the predetermined position, and then The first mixing device 11 is moved so that the sample stirring member 112 of the first mixing device 11 enters the sample container 91 grasped by the clamping claw 1201 and performs the stirring and mixing operation.
  • step S11 when the control device 21 determines that the sample container on the current sample rack 80 is filled with a trace amount of whole blood sample (or When pre-diluting the sample container 91 of the micro blood sample), the container pressing assembly 14 is controlled to move the two driven wheels 144a and 144b to clamp the sample container 91 on the sample rack 80, and control the sample stirring part of the first mixing device 11 112 Downward (Z direction) blood samples entering the sample container 91 for mixing operation; when the control device 21 determines that the sample container on the current sample holder 80 is a sample container containing a constant blood sample according to the coded information of the sample holder 80 label At 92 o'clock, the second mixing device 12 is controlled to drive the gripper 1201 to grab the sampling container 92 from the sample holder 80 to a certain position and rotate to perform the mixing operation on the blood sample in the sample container 92 (for example, by inverting and mixing) ).
  • control device 21 can also control the movement of the container pressing assembly 14 so that the two driven wheels 144a and 144b clamp the sample container 91 on the sample rack 80 and move to a certain position, and control the first mixing device
  • the sample stirring member 112 of 11 enters the blood sample in the sample container 91 downward (Z direction) and performs a mixing operation.
  • the sample container 91 (92) containing the mixed blood sample is transported to a first sampling position (not shown) of the blood sample analyzer (step S12), and then the process proceeds to step S13.
  • step S13 the control device 21 controls the sample suction needle 135 of the sample suction device 13 to suck a predetermined amount of blood sample from the sample container 91 (92) on the sampling position according to the received measurement mode information.
  • the aspiration needle 135 of the aspiration device 13 aspirates a first sample amount of blood sample from the first sample container 91; in the third measurement mode, the aspiration of the sample aspiration device 13 The sample needle 135 aspirates a blood sample of a third sample amount from the first sample container 91; in the second measurement mode, the sample needle 135 of the sample suction device 13 aspirates a second sample amount from the second sample container 92 Blood sample.
  • step S14 the sample preparation device of the blood sample analyzer 1 prepares a test sample from the blood sample aspirated by the sample suction device 13.
  • a first test sample is prepared from the first sampled blood sample aspirated
  • a pre-diluted blood sample is prepared from the third sampled liquid.
  • step S15 the detector of the blood sample analyzer 1 detects the detection sample prepared by the sample preparation device, and obtains a detection result.
  • the control device 21 determines whether there is an unprocessed next sample container 91 (92) on the sample rack (step S16), and if there is an unprocessed sample container 91 (92) (step S16: Yes), returns to step S8 for corresponding processing . If all the sample containers 91 (92) have been processed (step S16: NO), the first sampling mode is ended (step S17), and the process proceeds to step S26.
  • step S6 the sample compartment cover 181 is opened (step S18).
  • step S18 when the control device 21 is in the second injection mode, if the sample compartment cover 181 was originally closed, this step S18 is performed. If the sample compartment cover 181 is originally opened, the process proceeds directly to the next step S19.
  • step S19 the user selects the measurement mode of the current blood sample through the setting interface of the blood analyzer 1.
  • the control device 21 determines whether or not a start button (not shown) has been pressed (step S20). If the control device 21 determines that the start button has not been pressed (step S20: NO), it proceeds to step S26. If it is determined that the start button has been pressed (step S20: YES), the process proceeds to step S21, the sample compartment cover 181 is closed, and the process proceeds to step S22.
  • the control device 21 controls the aspiration needle 135 of the aspiration device 13 based on the measurement mode information selected by the user in step S19 to aspirate a predetermined amount of blood sample from the sample container 91 (92) on the second sampling position. .
  • the aspiration needle 135 of the aspiration device 13 aspirates a first sample amount of blood sample from the first sample container 91; in the third measurement mode, the aspiration of the sample device 13
  • the sample needle 135 sucks a third sample volume of the pre-diluted blood sample from the first sample container 91; in the second measurement mode, the sample needle 135 of the sample suction device 13 sucks the second sample container 92 Sampling volume of blood sample.
  • the first sampling amount is smaller than the second sampling amount, for example, the first sampling amount is 5-50 ⁇ L, and more preferably 15-35 ⁇ L.
  • step S23 the sample preparation device of the blood sample analyzer 1 prepares a test sample from the blood sample aspirated by the sample suction device 13.
  • a first test sample is prepared from the first sampled blood sample aspirated
  • a pre-diluted blood sample is prepared from the third sampled liquid.
  • step S24 the detector of the blood sample analyzer 1 detects the detection sample prepared by the sample preparation device, obtains a detection result, ends the second injection mode (step S25), and proceeds to step S26.
  • step S26 if no shutdown instruction is received (step S26: NO), return to step S2; if a shutdown instruction is received (step S26: YES), shutdown is performed (step S27), and the process ends.
  • red blood cells, white blood cells and platelets such as white blood cell count (WBC), red blood cell count (RBC), hemoglobin concentration (HGB), hematocrit (HCT), average red blood cell volume (MCV), average red blood cells Hemoglobin content (MCH), mean erythrocyte hemoglobin concentration (MVHC), platelet count (PLT), lymphocyte ratio (LY%), monocyte ratio (MONO), neutrophil ratio (NEUT%), lymphocyte (LY ), Monocyte count (MONO), neutrophil count (NEUT), red blood cell distribution width (RDW), platelet volume distribution width (PDW), mean platelet volume (MPV), and / or large platelet ratio (P-LCR ) And so on.
  • WBC white blood cell count
  • RBC red blood cell count
  • HGB hemoglobin concentration
  • HCT hematocrit
  • MCV average red blood cell volume
  • MCH average red blood cells Hemoglobin content
  • MVHC mean erythrocyte hemoglobin concentration
  • PTT lymphocyte ratio
  • FIG. 24 shows a schematic block diagram of a mixing operation in step S11.
  • the control device 21 compares the coded information read from the sample rack 80 tag with the preset rack information of the user (step S1101), and determines whether the read coded information matches the preset rack information. (Step S1102). If the read encoding information matches the preset sample rack information (step S1102: Yes), it is judged that the sample on the current sample rack is executed in the first or third measurement mode, and preset preset mixing parameters are set to set the first mixing device. 11 (step S1103), and then the control device 21 controls the first mixing device 11 to perform a blood sample mixing operation on the current sample container 91 used in the first measurement mode or the third measurement mode (step S1104).
  • step S1102 If the read encoding information does not match the preset sample rack information (step S1102: NO), it is judged that the sample on the current sample rack performs the second measurement mode, and the control device 21 controls the second mixing device 12 to the current sample container. 92 performs a blood sample mixing operation (step S1105).
  • the first mixing device 11 drives the sample stirring member 112 to perform the mixing operation on the blood sample in the sample container 91, and then cleans and dries the sample stirring member 112.
  • the sample stirring member 112 may be cleaned and air-dried using a cleaning swab (see FIG. 5).
  • the swab may be fixed and cleaned and air-dried by moving the sample agitating member 112, or the sample agitating member 112 may be fixed and cleaned and air-dried by moving the swab.
  • the cleaning component may further include a cleaning tank 114.
  • the cleaning component may also be cleaned by using one cleaning tank (see FIG. 25). That is, after the sample stirring member 112 finishes stirring, it moves into the washing tank 114 by itself, and cleans the sample stirring member 112 by means of liquid flushing in the washing tank 114 or the like.
  • FIG. 26 is a diagram illustrating an example of aspirating a sample in step S13 in the first injection mode in this embodiment.
  • the encoded information of the sample rack 80 label read by the container rotary scanning device is compared with the sample rack information preset by the user (step S131). Based on the comparison result, it is determined whether the current sample rack 80 is a micro whole blood sample rack (step S132). If it is a micro whole blood sample rack (step S132: Yes), the measurement mode of the device is set to the first measurement mode (step S133). ), And then the sample aspiration device 13 aspirates the first sampled blood sample from the sample container 91 on the sample rack 80 (step S134).
  • step S132 If it is not a micro whole blood sample rack (step S132: No), it is determined whether it is a pre-diluted micro blood sample rack (step S135). If it is a pre-diluted micro blood sample holder (step S135: YES), the measurement mode of the device is set to the third measurement mode (step S136), and then the sample suction device 13 sucks the second sample from the sample container 91 on the sample holder 80. Three samples of blood samples (step S137).
  • step S135 If it is not a pre-diluted micro blood sample holder (step S135: No), the measurement mode of the device is set to the second measurement mode (step S138), and then the sample suction device 13 sucks the first sample container 92 from the sample container 80 on the sample holder 80 Two samples of the blood sample (step S139).
  • FIG. 27 is a diagram illustrating an example of aspirating the sample in step S22 in the second injection mode in this embodiment.
  • the measurement mode selected by the user is the first measurement mode (step S221), and if it is the first measurement mode (step S221: Yes), the sample suction device 13 removes the sample container from the sample container 80 91 (92, 93) aspirates the first sampled blood sample (step S222). If it is not the first measurement mode (step S221: NO), it is determined whether it is the second measurement mode (step S223).
  • step S223 If it is the second measurement mode (step S223: YES), the sample suction device 13 sucks a second sample amount of blood sample from the sample container 94 on the sample rack 80 (step S224). If it is not the second measurement mode (step S223: NO), the sample suction device 13 sucks a blood sample of a third sampling amount from the sample container 91 (92, 93) on the sample rack 80 (step S225).
  • the structure of the blood analyzer in this embodiment differs from the blood analyzer 1 in the first embodiment in that the blood analyzer in this embodiment does not have the second mixing device 12 and the rest is the same as the blood analyzer in the first embodiment.
  • the corresponding parts in 1 have the same structure, so the same structural parts use the same reference numerals and descriptions are omitted.
  • FIGS 28 and 29 show the main flow of an example of a blood sample analyzed and processed by a blood sample analyzer.
  • Steps S421 to S430 and S432 to S447 are the same as the processing operations of steps S1 to S10 and S12 to S27 in the first embodiment, and a description thereof is omitted here.
  • step S431 the control device 21 controls the first mixing device 11 to move above the current sample container 91 for the first measurement mode or the third measurement mode, and then lowers the sample stirring member 112 of the first mixing device 11 and drives The sample stirring unit 112 performs a mixing operation on the blood samples in the sample container 91.
  • the control device 21 controls the movement of the container pressing assembly 14 such that the two driven wheels 144 a and 144 b clamp the sample container 91 (92) on the sample rack 80 and controls the first mixing device 11.
  • the sample stirring unit 112 enters the blood sample in the sample container 91 (92) downward (Z direction) and performs a mixing operation.
  • the blood analyzer has only the first mixing device 11.
  • the first mixing device 11 can be used for mixing a small amount of whole blood sample, a pre-diluted blood sample or a venous blood sample. Before the sample transport device 17 transports the sample rack 80, the container lid of the sample container 92 on the sample rack 80 is opened. Preferably, the first mixing device 11 is only used for mixing a small amount of whole blood sample and a pre-diluted blood sample.
  • the difference between the structure of the blood analyzer in this embodiment and the blood analyzer in Embodiment 2 is that the blood analyzer in this embodiment is provided with only the second sample injection mode, without the sample transport device 17, that is, without Set the first injection mode to make the blood analyzer more compact.
  • the rest of the structure is the same as that of the corresponding part of the blood analyzer according to the second embodiment. Therefore, the same components are denoted by the same reference numerals and descriptions thereof are omitted.
  • FIG. 30 is a main flow block diagram of an example of analyzing a blood sample by the blood sample analyzer 1.
  • the control device 21 starts initialization (step S501).
  • the initialization of the program and the initialization of the liquid circuit components of the blood sample analyzer 1, the cleaning of the pipeline, and the resetting of the driving part are performed.
  • step S502 a measurement mode is selected on a setting interface displayed on the display section 31.
  • the control device 21 determines whether a start button (not shown) has been pressed (step S503). If the control device 21 determines that the start button has not been pressed (step S503: No), it proceeds to step S510. If it is determined that the start button has been pressed (step S503: YES), the sample compartment cover 181 is closed (step S504). Regarding step S504, when the sample compartment cover 181 was originally closed, the process proceeds directly to the next step S505. If the sample compartment cover 181 is originally open, the step S504 is performed.
  • the control device 21 controls the first mixing device 11 or the second mixing device 12 to perform a mixing operation on the blood sample in the sample container 91 (92) (step S505).
  • the control device 21 determines that the current measurement mode is the first measurement mode, the second measurement mode, or the third measurement mode. If the control device 21 determines that the current measurement mode is the first measurement mode or the third measurement mode, the control The first mixing device 11 performs a blood sample mixing operation on the sample container 91 in the sample container fixing hole 182.
  • step S505 if the control device 21 determines that the current measurement mode is the second measurement mode, the stepping motor 1223 controlling the second mixing device 12 drives the gripper 1201 to grab the sampling container 92 from the sample container fixing hole 18 to a certain level. Position and rotate to perform the mixing operation of the blood sample on the current sample container 92.
  • the second mixing device may not be provided, and only a small amount of whole blood or a pre-diluted blood sample may be detected, thereby further miniaturizing the blood analyzer.
  • the control device 21 controls the sample suction needle 135 of the sample suction device 13 to suck a predetermined amount of blood sample from the sample container 91 (92) on the sampling position according to the measurement mode information selected by the user.
  • the aspiration needle 135 of the aspiration device 13 aspirates a first sample amount of blood sample from the first sample container 91; in the third measurement mode, the aspiration of the sample device 13
  • the sample needle 135 aspirates a blood sample of a third sample amount from the first sample container 91; in the second measurement mode, the sample needle 135 of the sample suction device 13 aspirates a second sample amount from the second sample container 92 Blood sample.
  • the first sampling amount in the first measurement mode is less than the second sampling amount in the second mode, for example, the first sampling amount is preferably 5-50 ⁇ L, and more preferably 15-35 ⁇ L.
  • the sample chamber cover 181 is opened, and the aspirated sample container 91 (92) is taken out (step S507).
  • the step of opening the sample compartment cover 181 and taking out the aspirated sample container 91 (92) can also be performed at any time after the completion of the blood sample aspiration process, and is not limited to the completion of the blood sample aspiration process Remove immediately.
  • step S508 the sample preparation device of the blood sample analyzer 1 prepares a test sample from the blood sample aspirated by the sample suction device 13.
  • a first test sample is prepared from the first sampled blood sample aspirated
  • a pre-diluted blood sample is prepared from the third sampled liquid.
  • a third test sample; and in the second measurement mode, a second test sample is prepared from the blood sample with which the second sample amount is aspirated.
  • the first sampling amount is smaller than the second sampling amount, for example, the first sampling amount is 5-50 ⁇ L, and more preferably 15-35 ⁇ L.
  • step S509 the detector of the blood sample analyzer 1 detects the detection sample prepared by the sample preparation device, obtains a detection result, and proceeds to step S510.
  • step S510 if no shutdown instruction is received (step S510: NO), return to step S502; if a shutdown instruction is received (step S510: YES), shutdown is performed (step S511), and the process ends.
  • the sample in the sample container 91 mixed by the first mixing device 11 may be a whole blood sample or a pre-diluted sample.
  • the whole blood sample may be a peripheral whole blood sample or a venous whole blood sample.
  • the first mixing device 11 performs a mixing operation only on a trace whole blood sample or a pre-diluted blood sample.
  • Hemoglobin concentration refers to the amount of hemoglobin contained in a unit volume of blood. Hemoglobin, also called hemoglobin, exists only in red blood cells and is the main component of red blood cells.
  • the red blood cell concentration in the lower part of the blood sample is higher than the upper part.
  • the sampling needle draws the sample near the bottom of the blood collection tube (the blood analyzer will reduce the blood collection requirements of the blood analyzer, the sampling needle will be close to Take blood samples from the bottom of the blood vessel), the hemoglobin concentration (HGB) measured by the blood analyzer will be significantly higher than the actual value, and the fluctuation of the hemoglobin concentration (HGB) measured several times will be large. Therefore, the stability of the hemoglobin concentration (HGB) measurement is often used to measure the effect of blood sample mixing.
  • the sample stirring member 112 of the first mixing device 11 is driven and driven by the mixing device driving motor into the sample container to perform the stirring operation.
  • the present application is not limited to this.
  • the sample transport device 17 can also transport the sample rack 80 containing the sample container to a predetermined position, and grasp and move the sample rack 80 or the sample rack 80 by the grippers 1201 of the second mixing device 12.
  • the sample container to the mixing position so that without moving the first mixing device 11, the sample stirring member 112 is relatively entered into the sample container, and the blood sample in the sample container is stirred by driving the sample stirring member 112 . In this way, the blood sample in the sample container is stirred, so that the mobile device of the first mixing device 11 can be omitted.
  • the sample stirring member 112 may also be mounted on a mechanism that can be moved up and down, and driven by a motor, a pulley or a screw rod to control the stirring bar to move up and down.
  • the sample stirring member 112 is mounted on the up-and-down moving mechanism through a bearing connection, so that the sample stirring member 112 can perform a rotation movement along the axis of the sample stirring member 112 while moving up and down.
  • the sample container 91 (92) is placed on the sample rack 80 and horizontally moved to a predetermined position (mixing position) by the sample transport device 17. After the sample container 91 (92) arrives, the sample stirring member 112 moves downward (as shown in the Z direction in FIG.
  • the sample transport device 17 advances the sample container 91 (92) to move the sample container 91 (92) to the blood collection position, and the analyzer starts collecting blood and analyzing.
  • Hemoglobin concentration is an important parameter for blood sample measurement. It refers to the amount of hemoglobin contained in a unit volume of blood. Hemoglobin, also called hemoglobin, exists only in red blood cells and is the main component of red blood cells. Blood is composed of blood cells and plasma. Because the specific gravity of blood cells is greater than the specific gravity of plasma, blood will stratify after standing for a period of time, in which blood cells sink below and plasma lies above.
  • the red blood cell concentration in the lower part of the blood sample is higher than the upper part.
  • the sampling needle draws the sample near the bottom of the blood collection tube (the blood analyzer will reduce the blood collection requirements of the blood analyzer, the sampling needle will be close to Take blood samples from the bottom of the blood vessel), the hemoglobin concentration (HGB) measured by the blood analyzer will be significantly higher than the actual value, and the fluctuation of the hemoglobin concentration (HGB) measured several times will be large. Therefore, the stability of the hemoglobin concentration (HGB) measurement is often used to measure the effect of blood sample mixing.
  • a blood sample of a subject is prepared with a sample for detection of a hemoglobin concentration (HGB) detection item by a sample preparation device, and an indicator related to the hemoglobin concentration (HGB) is obtained by the detector.
  • HGB hemoglobin concentration
  • FIG. 31 is a graph showing the analysis data of 6 whole blood samples of 100 ⁇ L each.
  • the data was mixed by the mixing device 11 and measured six times in the first measurement mode. From the data in FIG. 31, the fluctuation range of the hemoglobin concentration (HGB) was only 1 g / L, which was very stable.
  • HGB hemoglobin concentration
  • Figure 32 shows six first sample containers 91 each containing 30 microliters, 50 microliters, 100 microliters, 150 microliters, 200 microliters, and 250 microliters of different whole blood, mixed by a mixing device 11 and measured six times in the first measurement mode using HGB It can be seen from the data that the fluctuation range of hemoglobin concentration (HGB) does not exceed ⁇ 2g / L, which meets the measurement requirements.
  • HGB hemoglobin concentration
  • the second mixing device 12 can grab the sample container on the sampling rack 80 and drive the sample container containing constant blood to perform upside-down mixing.
  • the second mixing device 12 can also grab the sample rack 80 and drive all the sample containers with constant blood on the sample rack 80 to mix upside down.
  • the mixing position refers to a position where the first mixing device 11 or the second mixing device 12 mixes the blood samples in the sample container.
  • the first mixing position is the same position as the predetermined position.
  • FIG. 33 is a schematic structural diagram of a mixing device according to this embodiment.
  • the sample transport device 17 or the sample bin assembly 18 transports the sample container 91 (92) to the sampling position.
  • the sampling position refers to a position where the sampling needle 205 (135) performs sampling.
  • the aspiration device 13 moves the aspiration needle 205 into the sample container 91 (92), and drives the aspiration needle 205 to inhale an appropriate amount of blood sample, and then returns the inhaled blood sample to the sample container 91 (92), so that the sample container
  • the blood samples in 91 (92) formed a certain flow, and the blood samples were mixed well.
  • FIG. 34 is a schematic structural diagram of a sample suction device according to this embodiment. As shown in FIG. 34, the sample suction device 20 is used to mix the blood samples of the sample container 91 (92) sent to the sampling position of the analyzer 1 by the sample transport device 17 and draw an appropriate amount of blood from the mixed blood samples. Samples are used for sample preparation.
  • the suction device 20 includes: a suction needle 205, a suction needle moving assembly 201, a stepper motor 2001, a synchronous wheel 2002 and 2003, an annular synchronous toothed belt 2004 wound around the synchronous wheel 2002 and 2003, and along the Y1 and Y2 directions
  • the suction and suction driving device is used to drive the suction needle 205 to suck an appropriate amount of blood sample, and then return the sucked blood sample to the sample container 91 (92), so that the blood sample in the sample container 91 (92) forms a certain amount.
  • the suction drive device is a syringe.
  • the suction needle moving assembly 201 is connected to the endless synchronous toothed belt 2004 through a connecting member.
  • the endless synchronous toothed belt 2004 is driven by the rotation of the stepper motor 2001 and rotates under the guidance of two synchronous wheels 2002 and 2003.
  • the sucking needle moving component 201 can drive the sucking needle 205 to move in the Y1 or Y2 direction under the driving of the stepping motor 2001.
  • the initial position of the suction needle moving component 201 in the Y1 and Y2 directions is positioned by the position sensor 2006 and the sensor sensing piece 2018 fixed on the suction needle moving component 201.
  • the sample needle moving assembly 201 includes: a stepper motor 2011, a screw 2012, a nut 2023, a linear slide 2014, a sample needle fixing member 2015, a position sensor 2016, and a sensor induction piece 2017, and a needle position sensor (not shown) )Wait.
  • the aspiration needle position sensor is used to sense the downward position of the aspiration needle 205 to prevent the needle tip of the aspiration needle 205 from continuing to descend after reaching the bottom of the sample container 91 (92), resulting in the needle tip of the aspiration needle 205 or the sample container 91. (92) Damaged.
  • the aspiration needle 205 is fixed on the aspiration needle fixing part 2015, and the aspiration needle fixing part 2015 is fixed on the linear slide rail 2014 placed along Z1 and Z2 by screws, and the nut 2013 is stuck on the aspiration needle fixing part 2015. There is no relative rotation between the nut 2013 and the suction needle fixing member 2015 in the slot.
  • the lead screw 2012 is connected to the rotating shaft of the stepper motor 2011 by screws.
  • the stepper motor 2011 can drive the screw 2012 to rotate and drive the suction needle fixing member 2015 to move the suction needle 205 in the Z1 or Z2 direction.
  • the initial position of the suction needle 205 in the Z1 and Z2 directions is positioned by the position sensor 2017 and an optical coupling sensor sensing piece (not shown) provided on the suction needle fixing member 2015, and the suction of the sample is performed by the suction needle position sensor.
  • the needle 205 is moved and positioned in the Z1 or Z2 direction to prevent the needle tip of the suction needle 205 from continuing to descend after reaching the bottom of the sample container 91 (92).
  • the suction needle 205 can move in two directions in the Y1, Y2 directions and Z1, Z2 directions.
  • the functions of mixing blood samples in a sample container by suction and spitting, and drawing an appropriate amount of blood samples from the mixed blood samples, and dividing blood samples into a sample preparation device can be realized.
  • the step of mixing the blood sample in the sample container 91 (92) through the suction needle 205 is as follows:
  • the control device 21 determines whether the sampling mode is the first sampling mode or the second sampling mode
  • the sample container 91 (92) on the sample rack 80 is transported to the first sampling position by the sample transport device 17; if it is the second sampling mode, a single sample is transferred by the sample compartment assembly 18 The container 91 (92) is transported to a second sampling position, where the first sampling position and the second adoption position may be the same position or different positions;
  • the outer wall of the suction needle 205 is air-dried by the suction needle air-drying device, and the suction driving device drives the suction needle 205 to suck an appropriate amount of air, so that a section of isolated air column is formed inside the suction needle 205;
  • the aspiration needle moving assembly 201 drives the aspiration needle 205 downward, and the aspiration needle position sensor or the aspiration needle driving device determines whether the needle tip of the aspiration needle 205 reaches the bottom of the sample container 91 (92) according to the number of motor steps.
  • the tip of the needle 205 reaches the bottom of the sample container 91 (92), and the aspiration needle moving assembly 201 stops driving the aspiration needle 205 downward, otherwise it continues to drive the aspiration needle 205 downward until it reaches the bottom of the sample container 91 (92);
  • the suction driving device drives the sampling needle 205 to suck an appropriate amount of blood sample, and then returns the sucked blood sample to the sample container 91 (92), so that the blood sample in the sample container 91 (92) forms a certain flow until the blood Sample mixing
  • the aspiration needle 205 draws an appropriate amount of the mixed blood sample from the sample container 91 (92) to collect the blood sample.
  • the whole sample is contained in the sample container 91 (92), and since the whole blood sample is directly sucked and mixed by using the suction needle 205, the suction needle 205 can be directly sucked after mixing A predetermined volume of whole blood sample is sufficient, and the sampling needle does not need to be cleaned.

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Abstract

本申请公开了一种血样分析仪，包括：运样装置，用于运送装有样本容器的样本架；第一混匀装置，具有用于搅拌所述样本容器中的血液样本的样本搅拌部件，所述第一混匀装置能够驱动所述样本搅拌部件对所述样本容器的血液样本进行混匀；第二混匀装置，能够获取所述样本架或所述样本架上的所述第二样本容器，并能够驱动所述第二混匀位上的装有常量血液样本的第二样本容器以进行血液样本的混匀；控制装置，与所述运样装置、所述第一混匀装置和所述第二混匀装置通信连接，控制所述运样装置、所述第一混匀装置和所述第二混匀装置的动作。此外，本申请还公开了一种血样混匀装置、血样分析方法、以及一种计算机存储介质。

Description

血样分析仪、血样分析方法及计算机存储介质 技术领域
本申请涉及样本分析领域，尤其涉及一种对抽取的微量样本进行混匀和分析的血样分析仪以及血样混匀方法。
背景技术
在临床诊断过程中，经常需要用分析装置测定从患者采集的血液、尿液、体液(腹水、脑脊髓、胸水等)样本。通常分析装置都会事先规定所需的样本量。以血液样本为例，目前采血方式有两种：采静脉血和采末梢血。静脉采方式采血量多(≥1mL)，通常适合于成年患者；而对于婴幼儿、儿童或重症患者有时难以通过静脉方式采血，这种情况下往往采末梢血，采末梢血面临的情况是能采集到的血量较少(大部分情况≤100μL)。
采血时，为了防止血液凝固，通常会采用含有抗凝剂的采血管。血液由血细胞和血浆构成，由于血细胞和血样的比重不同，抗凝后的血液静置一段时间后会产生分层，因此测量前需先将血样充分混匀，否则测量结果会产生较大偏差。
CN1334453A公开了一种用于处理血液制品样品的装置，该装置具有用于搅拌试管中血液样本的摇动装置，该摇动装置用夹紧组件夹持试管，并旋转夹紧组件使试管以360°连续旋转，以此连续地上下倒置试管，从而倒置搅拌试管内的血液样本，以此搅拌试管中的血液样本。
对于常量血液样本(静脉血样本)，由于采血量大，血液的流动性好，在采血管颠倒时，静脉血在重力作用下必然沿管壁流动，可以采用CN1334453A所公开的多次颠倒方式，使血液沿管壁往复流动实现混匀。
在CN1334453A中，对常量的血液样本和微量血液样本采用同样的搅拌作业。然而，采用CN1334453A公开的颠倒混匀方式会使部分血液残留在采血管帽及管壁上而造成血样损失，这对于静脉血样本之类常量样本而言，损失的血样占总采血量的比重很小，对测量不造成影响。但是，对末梢血样本之类的微量样本而言，因为末梢血样本采血量少，流动性差，采血管颠倒时末梢血往往黏附在采血管帽、采血管底部或管壁上不流动，上述在先技术公开的颠倒混匀技术均会造成血样损失，对测量造成不利影响，并且仍然存在难以有效解决末梢血混匀难题。因此，采用CN1334453A公开的颠倒混匀方式，即使对常量的血液样本能够充分搅拌均匀，但对于微量血液样本可能存在搅拌欠佳的情况。
对于上述CN1334453A中搅拌均匀欠佳的技术问题，CN103675309A公开了一种样本处理装置，该处理装置具有搅拌电机部件和手部件，通过搅拌电机部件来驱动手部件旋转，使得样本容器在倒置状态和直立状态之间旋转。在CN103675309A中，为了区分常量血液样本和微量血液样本之间搅拌均匀的要求不同，采用了使在微量血液样本模式下搅拌样本的时间长于在常量血液样本模式下搅拌样本的时间，以使得微量血液样本能够得到充分的搅拌。
虽然，在CN103675309A中，采用了使在微量血液样本模式下搅拌样本的时间长于在常量血液样本模式下搅拌样本的时间，以使得微量血液样本能够得到充分的搅拌。但是，仍然存在微量血液样本往往黏附在采血管帽或管壁上部的情况。
由此，采用上述在先技术公开的颠倒混匀技术混匀末梢血要面临两个问题：(1)目前国内绝大部分末梢血采血管均采用塑料帽，塑料帽不支持穿刺(直接穿刺塑料帽会损坏穿刺针，以及塑料碎屑会掉入采血管中污染血样)，因此测量前需要先打开采血管帽，颠倒采血管会使血样流出来；(2)即使国外有一些厂家提供带橡胶帽的采血管可支持穿刺，但一来进口采血管成本相对较高无法普及，更严重的问题是颠倒采血管会使部 分血样残留在橡胶帽上造成血样损失，由于末梢血采血量本身就少，损失的血样占总体采血量的比重大，极容易引起分析仪吸样不足而影响测量结果。
CN107121559A公开了一种将末梢血样本和稀释液的混合液进行混匀的方法，在该方法中：将采样针插入混合液离心管中，通过采样针的自动吸入和吐出的方法对混合液进行混匀操作。
对于全血样本，并不是匀质液体，而是由血浆(一般占体积55％左右)和血细胞(一般占体积45％左右)组成。血细胞可以理解为微小颗粒，其密度一般情况下比血浆略大。因此，如果全血样本在加了抗凝剂(防止血液凝结)并静置一段时间后，血液样本在采血管92中会出现分层现象：血浆在上层，血细胞在下层(参照图1)。如果采用CN107121559A所公开的自动吸入和吐出的方法对全样血进行混匀时，因血液样本出现分层而使得采样针处于血浆层或血细胞层，在采样针进行吸入和吐出操作时，也往往是在所处血浆层或血细胞层中的某一层进行，难以将血浆层和血细胞层进行充分地混匀，而且采样针每次吸入和吐出的量较小，需要较长的时间进行混匀操作。
基于上述在先技术公开的样本混匀技术所存在的技术问题，以及市场对末梢血全自动化测量的迫切需求，本申请提出一种样本分析仪和样本混匀方法，该装置和方法能够有效实现对末梢血样本之类的微量全血样本进行搅拌均匀。
发明内容
根据本申请的第一方面，提供了一种血样分析仪，包括：运样装置，用于运送装有第一和/或第二样本容器的样本架；第一混匀装置，具有用于搅拌第一样本容器中的血液样本的样本搅拌部件，所述第一混匀装置能够驱动所述样本搅拌部件对第一混匀位的所述样本架上的装有微量血液样本的所述第一样本容器中的血液样本进行混匀；第二混匀装置，能够获取所述样本架或所述样本架上的所述第二样本容器，并能够驱动第二混匀位上的装有常量血液样本的第二样本容器以进行血液样本的混匀；控制装置，与所述运样装置、所述第一混匀装置和所述第二混匀装置通信连接，控制所述运样装置、所述第一混匀装置和所述第二混匀装置的动作。
本申请的第二方面，提供了一种血样分析仪，包括：样本仓组件，具有样本仓盖和样本容器固定孔，用于放置在所述样本容器固定孔上的微量血样或常量血样的单样本进样；混匀装置，具有用于搅拌所述样本容器中的血液样本的样本搅拌部件，所述混匀装置能够驱动所述样本搅拌部件对所述样本容器的血液样本进行混匀。
本申请的第二方面，提供了一种血样分析仪，包括：第一混匀装置，能够对第一样本容器中的血液样本进行混匀；第二混匀装置，能够以不同于所述第一混匀装置的方式对所述第二样本容器中的血液样本进行混匀；控制装置，与所述第一混匀装置和所述第二混匀装置通信连接，并能够执行以下作业：(1)判断是第一测量模式还是第二测量模式；(2)判断是所述第一测量模式时，控制所述第一混匀装置对所述第一样本容器中的血液样本进行混匀；(3)判断是所述第二测量模式时，控制所述第二混匀装置对所述第二样本容器中的血液样本进行混匀。
本申请的第三方面，提供了一种血样分析仪，包括：运样装置，用于运送装有样本容器的样本架；混匀装置，具有用于搅拌所述样本容器中的血液样本的样本搅拌部件，所述混匀装置能够驱动所述样本搅拌部件对所述样本容器的血液样本进行混匀；控制装置，与所述运样装置和所述混匀装置通信连接，控制所述运样装置和所述混匀装置的动作。
本申请的第四方面，提供了一种用于血常规的血样分析方法，包括：将装有血液样本的样本容器运送至混匀位；驱动第一混匀装置的样本搅拌部件对所述样本容器中的血液样本进行混匀；从所述混匀位上的所述样本容器中吸移预定采样量的所述血液样本制备血常规检测项目用的检测用试样；检测所述检测用试样，获取所述血常规检测项目的 相关指标。
本申请的第五方面，提供了一种血样分析方法，包括：测量模式确定步骤：判断当前测量模式是第一测量模式还是第二测量模式；第一检测用试样制备步骤：当判断是所述第一测量模式时，控制第一混匀装置驱动样本搅拌部件对样本容器中的血液样本进行混匀，并吸移第一采样量的血液样本制备第一检测用试样；第二检测用试样制备步骤：当判断是所述第二测量模式时，控制第二混匀装置对所述样本容器中的血液样本进行混匀，并吸移第二采样量的血液样本制备第二检测用试样；及检测步骤：检测所述第一检测用试样或所述第二检测用试样。
本申请的第六方面，提供了一种血样分析仪，包括：运样装置，用于运送装有样本容器的样本架；混匀装置，具有用于吸吐样本容器中的血液样本的吸样装置，所述吸样装置能够驱动所述吸样装置的吸样针对采样位上装有微量血液样本的所述样本容器中的血液样本进行吸吐混匀；控制装置，与所述运样装置、所述混匀装置通信连接，控制所述运样装置、所述混匀装置的动作。
本申请的第七方面，提供了一种血样混匀方法，包括：吸样针吸取适量空气，使所述吸样针内部形成一段隔离气柱；驱动所述吸样针下行，靠近样本容器的底部；驱动所述吸样针吸入适量的血液样本后，将所吸入的所述血液样本回退至所述样本容器中，使得所述样本容器中的所述血液样本形成一定流动，直至所述血液样本混匀。
本申请的第八方面，提供了一种用于血样分析仪的控制装置，包括：至少一个处理器；以及存储器，存储所述至少一个处理器可执行的指令，所述指令在被所述至少一个处理器执行时使得所述血样分析仪执行以上任一项所述的方法。
本申请的第八方面，提供了一种计算机存储介质，存储有计算机可执行指令，所述计算机可执行指令在血样分析仪的至少一个处理器执行时，使得所述血样分析仪执行以上任一项所述的方法。
进一步地，所述运样装置能够将装有第一和/或第二样本容器的样本架运送至所述第一混匀位；所述第二混匀装置能够从所述第一混匀位获取所述样本架或所述样本架上的所述第二样本容器运送至所述第二混匀位。
进一步地，所述第一混匀位与所述第二混匀位为同一位置。
进一步地，所述样本搅拌部件的头部为圆柱型、桨状或多边形。
进一步地，所述控制装置控制所述样本搅拌部件以自转、圆周轨道、线性摆动或上下震荡方式中一种或几种方式的组合进行搅拌。
进一步地，所述样本搅拌部件能够进行上下移动，并能向下移动到所述第一混匀位上的第一样本容器中进行搅拌混匀。
进一步地，所述的血样分析仪还包括：清洗部件，用于对所述样本搅拌部件进行清洗作业；优选地，所述清洗部件包括清洗液进口和清洗液排出口，以对位于所述清洗部件中的所述样本搅拌部件进行清洗作业；更优选地，所述清洗液排出口还能用于进行抽气，以此对所述样本搅拌部件进行干燥处理。
进一步地，所述清洗部件包括能够对所述样本搅拌部件进行清洗的清洗池。
进一步地，所述的血样分析仪还包括：样本仓组件，包括样本仓盖和样本容器固定孔，用于放置在所述样本容器固定孔上的微量血样或常量血样的单样本进样。
进一步地，所述的血样分析仪还包括：所述控制装置还用于判断当前进样模式是第一进样模式还是第二进样模式；当判断是所述第一进样模式时，控制所述运样装置运送装有所述第一和/或第二样本容器的所述样本架；当判断是所述第二进样模式时，控制所述样本仓组件运送单个所述第一和/或第二样本容器。
进一步地，所述运样装置将装有所述第一样本容器的样本架运送至预定位置，所述第二混匀装置的夹爪能够从所述预定位置的所述样本架上抓取所述第一样本容器到所述第一混匀位。
进一步地，所述的血样分析仪还包括：测量模式设定装置，用于设置第一测量模式和第二测量模式；其中，所述控制装置根据所述测量模式设定装置的设定执行以下动作：(1)判断是所述第一测量模式还是所述第二测量模式；(2)当判断是所述第一测量模式时，控制所述第一混匀装置对所述第一样本容器中的血液样本进行混匀；(3)当判断是所述第二测量模式时，控制所述第二混匀装置抓取所述第二样本容器进行混匀。
进一步地，所述的血样分析仪还包括：吸样装置，用于从样本容器吸移经过混匀后的血液样本；当判断是所述第一测量模式时，所述控制装置控制所述吸样装置从所述第一样本容器中吸移第一采血量的血液样本；当判断是所述第二测量模式时，所述控制装置控制所述吸样装置从所述第二样本容器中吸移第二采血量的血液样本；其中，所述第一采样量少于所述第二采样量；优选的所述第一采样量为5-50μL，更优选的为15-35μL。
进一步地，所述测量模式设定装置还用于设置第三测量模式；当所述控制装置判断是所述第三测量模式时，控制所述第一混匀装置对所述第一样本容器中预稀释的血液样本进行混匀。
进一步地，所述第一混匀装置对所述第一样本容器的微量全血样本进行混匀；优选的，所述第一样本容器的微量全血样本为30-250μL，更优选的为50-200μL，更更优选地为50-100μL。
进一步地，所述第一混匀装置对所述第一样本容器的微量血液样本进行了混匀后，多次重复测量该微量血液样本的血红蛋白值的波动幅度不超过±2g/L。
进一步地，所述血样分析仪仅用于处理微量全血血样本和预稀释血样本。
进一步地，所述的血样分析仪还包括：吸样装置，用于从所述样本容器吸移经过混匀后的血液样本；制样装置，用于将所述吸样装置吸移的所述血液样本制备成检测用试样；控制装置，与所述混匀装置、所述吸样装置和/或所述制样装置通信连接，控制所述混匀装置、所述吸样装置和/或所述制样装置的动作。
进一步地，所述的血样分析仪还包括：测量模式设定装置，用于设置第一测量模式和第二测量模式；其中，所述控制装置根据所述测量模式设定装置的设定执行以下动作：(1)判断是所述第一测量模式还是所述第二测量模式；(2)判断是所述第一测量模式时，控制所述吸样装置从所述样本架上的所述样本容器中吸移第一采样量的所述血液样本，并控制所述制样装置制备第一检测用试样；(3)判断是所述第二测量模式时，控制所述吸样装置从所述样本架上的所述样本容器中吸移第二采样量的所述血液样本，并控制所述制样装置制备第二检测用试样；其中，所述第一采样量少于所述第二采样量；优选的所述第一采样量为5-50μL，更优选的为15-35μL。
进一步地，所述测量模式设定装置还用于设置第三测量模式；当所述控制装置判断是所述第三测量模式时，控制所述血液分析仪对已预稀释血液样本，然后控制所述吸样装置从所述样本架上的所述样本容器中吸移第三采样量的所述预稀释血液样本，并控制所述制样装置制备第三检测用试样。
进一步地，所述控制装置还能够执行以下作业：(1)判断是否为第三测量模式；(2)当所述控制装置判断是所述第三测量模式时，控制所述第一混匀装置对所述样本容器中预稀释的血液样本进行混匀。
进一步地，所述血液样本为全血样本。
进一步地，所述的血样分析仪还包括：样本仓组件，具有样本仓盖和样本容器固定孔，用于放置在所述样本容器固定孔上的血液样本的单样本进样；和/或运样装置，用于运送装有所述第一样本容器和/或所述第二样本容器的样本架。
进一步地，所述控制装置还能够执行以下作业：(1)判断是第一进样模式还是第二进样模式；(2)判断是所述第一进样模式时，控制所述运样装置运送装有所述样本容器的所述样本架；(3)判断是所述第二进样模式时，控制所述样本仓组件向所述血液分 析仪运送单个所述样本容器。
进一步地，所述的血样分析方法还包括：判断当前测量模式是第一测量模式还是第二测量模式；当判断是所述第一测量模式时，从第一样本容器中吸移第一采样量的混匀后血液样本，并制备第一检测用试样；当判断是所述第二测量模式时，从第二样本容器中吸移第二采样量的混匀后血液样本，并制备第二检测用试样；其中，所述第一采样量少于所述第二采样量；优选的所述第一采样量为5-50μL，更优选的为15-35μL。
进一步地，所述的血样分析方法还包括：判断当前测量模式是否为第三测量模式；当判断是所述第三测量模式时，从第一样本容器中吸移第三采样量的混匀后的预稀释血液样本，并制备第三检测用试样。
进一步地，所述的血样分析方法还包括：判断当前进样模式是第一进样模式还是第二进样模式；当判断是所述第一进样模式时，由运样装置运送所述样本容器；当判断是所述第二进样模式时，由样本仓组件向血液分析仪运送单个所述样本容器。
进一步地，所述的血样分析方法还包括：第二混匀装置获取第二样本容器进行颠倒混匀。
进一步地，在所述测量模式确定步骤中，还判断所述当前测量模式是否为第三测量模式；第三检测用试样制备步骤：当判断是所述第三测量模式时，控制所述第一混匀装置对所述样本容器中的预稀释的血液样本进行混匀，并吸移第三采样量的所述预稀释血液样本制备第三检测用试样；在所述检测步骤中，检测所述第三检测用试样。
进一步地，所述的血样分析方法还包括：进样模式确定步骤：判断当前进样模式是第一进样模式还是第二进样模式；样架运送步骤：当判断是所述第一进样模式时，控制运样装置将装有所述样本容器的所述样本架运送至预定位置，并将混匀后的样本容器运送至第一采样位；样本仓组件关闭步骤：当判断是所述第二进样模式时，关闭样本仓组件，所述样本容器被送至第二采样位。
进一步地，在第一检测用试样制备步骤中，第二混匀装置将由运样装置运送至预定位置的样本架上的样本容器搬运至第一混匀位置进行混匀；在第二检测用试样制备步骤中，由第二混匀装置抓取由运样装置运送至预定位置的样本架或所述样本架上的所述样本容器进行颠倒混匀。
进一步地，所述吸样装置还包括吸吐驱动装置，用于驱动所述吸样针吸吐所述样本容器中的血液样本进行混匀。
进一步地，所述吸吐驱动装置为注射器。
进一步地，所述血液分析仪包括可以对常量血液样本进行颠倒混匀的混匀装置。
进一步地，所述吸吐驱动装置能够驱动所述吸样针在对所述样本容器中的血液样本进行混匀前吸取适量空气，使所述吸样针内部形成一段隔离气柱。
进一步地，所述吸样装置还包括吸样针风干装置，用于将所述吸样针的外壁风干。
进一步地，所述吸样装置还包吸样针位置传感器，用于感知所述吸样针的下行位置。
进一步地，所述的血样混匀方法还包括：判断进样模式是第一进样模式还是第二进样模式；如果是所述第一进样模式，则由运样装置将样本架上的所述样本容器运送至第一采样位；如果是所述第二进样模式，则由样本仓组件将单个所述样本容器运送至第二采样位。
进一步地，所述的血样混匀方法还包括：在所述吸样针吸取适量空气前，将所述吸样针的外壁风干。
基于上述技术方案所公开的装置和方法能够有效实现对末梢血样本之类的微量样本进行搅拌均匀，并可同时解决血样残留在橡胶帽上造成如末梢血等微量血样的损失而引起分析仪吸样不足而影响测量结果的技术问题，还能够使分析仪器在解决现有技术问题的情况下实现小型化。
附图说明
图1为血液样本在采血管中出现分层现象示意图；
图2为本申请一实施方式的血样分析仪的外观斜视图；
图3和图4为本申请一实施方式的运样装置的结构示意图；
图5为本申请一实施方式的第一混匀装置的结构示意图；
图6为本申请一实施方式的第二混匀装置的结构示意图；
图7为本申请一实施方式的吸样装置的结构示意图；
图8～11为本申请一实施方式的容器旋转扫码装置结构示意图；
图12～13为本申请一实施方式的容器旋转扫码装置工作原理示意图；
图14为本申请的样本架结构示意图；
图15为本申请的装有样本容器时样本架示意图；
图16为本申请的装有微量采血管时样本架示意图；
图17为本申请的一适配器结构示意图；
图18为本申请的另一适配器结构示意图；
图19为本申请控制装置的结构框图；
图20和图21为本申请血样分析仪分析处理血液样本一例的主要流程框图；
图22为本申请血液分析仪的设置界面示意图；
图23为本申请一实施方式中第一混匀装置的混匀作业示例图；
图24为本申请一实施方式中步骤S11中混匀作业的流程示意框图；
图25为本申请一实施方式中第一混匀装置的搅拌部件清洗示例图；
图26为本申请一实施方式中第一进样模式下的步骤S13吸移样本流程例示图；
图27为本申请一实施方式中第二进样模式下的步骤S22吸移样本流程例示图。
图28和图29为本申请血样分析仪分析处理血液样本另一例的主要流程框图；
图30为本申请血样分析仪分析处理血液样本又一例的主要流程框图；
图31为100μL的微量全血血样分析数据图示；
图32为不同体积的微量全血样本各6支用本申请第一实施方式的第一混匀装置11混匀，用第一测量模式检测HGB的数据图示；
图33为本申请一实施方式中混匀装置的结构示意图；
图34为本申请一实施方式的另一吸样装置的结构示意图。
具体实施方式
下面详细描述本申请的实施例，所述实施例的示例在附图中示出，其中自始至终相同或类似的标号表示相同或类似的元件或具有相同或类似功能的元件。下面通过参考附图描述的实施例是示例性的，仅用于解释本申请，而不能解释为对本申请的限制。
本技术领域技术人员可以理解，除非特意声明，这里使用的单数形式“一”、“一个”、“所述”和“该”也可包括复数形式。应该进一步理解的是，本申请的说明书中使用的措辞“包括”是指存在所述特征、整数、步骤、操作、元件和/或组件，但是并不排除存在或添加一个或多个其他特征、整数、步骤、操作、元件、组件和/或它们的组。应该理解，当我们称元件被“连接”到另一元件时，它可以直接连接到其他元件，或者也可以存在中间元件。此外，这里使用的“连接”可以包括无线连接。这里使用的措辞“和/或”包括一个或更多个相关联的列出项的全部或任一单元和全部组合。
本技术领域技术人员可以理解，除非另外定义，这里使用的所有术语(包括技术术语和科学术语)，具有与本申请所属领域中的普通技术人员的一般理解相同的意义。还应该理解的是，诸如通用字典中定义的那些术语，应该被理解为具有与现有技术的上下文中的意义一致的意义，并且除非像这里一样被特定定义，否则不会用理想化或过于正式的含义来解释。
考虑到末梢血样本采血量少，流动性差，采血管颠倒时末梢血往往黏附在采血管底 部或管壁上不流动，且颠倒混匀容易造成血样泼洒或血样的损失，传统的颠倒混匀技术难以解决末梢血混匀难题。基于此，本申请提出一种微量样本自动混匀方法、混匀装置和以及具备微量样本自动混匀功能的分析仪，其通过驱动搅拌部件在样本容器中运动的方式来实现微量样本的混匀。
(实施方式1)
图2为本实施方式的血样分析仪的外观斜视图。如图2所示，血样分析仪1包括仪器主体、机壳30、配置于仪器主体前面的运样装置17等。其中，在机壳30上设置有显示部件31、操作按钮32和操作按钮33等，显示部件31可以采用可触摸操作的可触摸屏，通过触摸显示部件31可以显示输入设备23(例如软键盘等)(参照图19)。在本实施方式中，也可以输入设备23作为硬件独立设置。
上述仪器主体可基本容纳于机壳30中，包括混匀样本容器(采血管)91中的血样的第一混匀装置11和样本容器(采血管)92中的血样的第二混匀装置12、从样本容器91(92)中吸移经第一混匀装置11或第二混匀装置12混匀的血样的吸样装置13、获取运样装置17运送至扫码位(未图示)的样本架80及样本容器91(92)标签上的样本编码信息的容器旋转扫码装置(包括容器压紧组件14、容器旋转组件15、扫码器16)、由吸样装置13吸移的血样制备检测用试样的制样器(未图示)、从制样器制备的检测用试样中检测血液中的血细胞的检测器(未图示)、可与显示部件31、操作按钮32、操作按钮33和仪器主体的相应部件通电连接的控制装置21等。
在本实施方式中，血样分析仪1还可以包括样本仓组件18，用于微量全血样或常量血样的单样本进样，通常用于急诊插队样本的测量。样本仓组件18具有样本仓盖181和样本容器固定孔182。当需要测量急诊插队样本时，可以通过打开样本仓盖181，用于将装有急诊插队样本的样本容器放入样本容器固定孔182，以固定样本容器，或从样本容器固定孔182取出样本容器。样本容器固定孔182的孔径略大于需要放入的样本容器或适配器81(82)(参见图17和图18)的外径。
图3和图4为本实施方式的运样装置的结构示意图。如图3所示，运样装置17包括：样本架支撑部件171，样本架送入装置172，样本架双向运样装置173、样本架送出装置174。
样本架支撑部件171包括：可以放置若干固定有装有分析前样本的样本容器的样本架80的分析前样本架存放区1711，可以存放若干固定有装有分析后样本的样本容器的样本架80的分析后样本架存放区1712，以及位于分析前样本架存放区1711和分析后样本架存放区1712之间的样本分析区(无图示)。在分析前样本架存放区1711一侧有一个样本架送入转向区1711a，在分析后样本架存放区1712有一个样本架送出转向区1712a。
样本架送入装置172有样本架送入部件1721和1722，样本架送入部件1721和1722通过向Y2方向移动，可以将存放在分析前样本架存放区1711的样本架80逐个推到样本架送入转向区1711a。样本架送入部件1721和1722由无图所示的步进电机驱动。进入样本架送入转向区1711a的样本架80将由样本架双向运样装置173继续沿X1方向运送。进入分析区的装有样本的样本容器91(92)将依次运送至扫码位由容器旋转扫码装置14～16进行扫码，然后送到预定位置由第一混匀装置11对样本容器91进行混匀或由第二混匀装置12将样本容器92抓取到一定位置进行混匀，再然后送到采样位由吸样装置13从样本容器91(92)中吸移经第一混匀装置11或第二混匀装置12混匀的血样、由制样器将吸样装置13吸移的血样制备成检测用试样、以及由检测器对制样器制备的检测用试样中的血细胞进行检测。
固定有装有样本的样本容器的样本架80被样本架双向运样装置173运送至样本架送出转向区1712a后，样本架送出装置174的样本架送出部件1741会沿Y1方向水平移动，将样本架80推移到分析后样本架存放区1712。样本架送出部件1741由无图所示的步进电机驱动。
图5为本实施方式的第一混匀装置的结构示意图。第一混匀装置11可以移动地安装在血样分析仪1的机壳30或其它支架上(未图示)，并通过电机驱动可以实现上下、左右移动或转动。如图5所示，第一混匀装置11包括：搅拌部件驱动电机111、样本搅拌部件112、清洗部件113。其中，搅拌部件电机111可以是步进电机、伺服电机或直流电机。在本实施方式中，优选地，上述电机111为步进电机。
样本搅拌部件112可以是头部为圆柱型、桨状、多边形等等形状的搅拌棒，并通过搅拌部件电机111的驱动以自转、圆周轨道、线性摆动或上下震荡等方式中的一种或几种方式的组合进行搅拌动作。同时，样本搅拌部件112在混匀装置驱动电机(未图示)的驱动下可以驱动混匀装置11进行上下、左右移动。
用于对样本搅拌部件112进行清洗作业的清洗部件113。在本实施方式中，优选地，清洗部件113包括清洗液进口1131和清洗液排出口1132。在清洗部件113完成对一个血液样本容器91中的血液样本的搅拌作业时，通过清洗部件113对位于其中的样本搅拌部件112进行清洗作业，以防对下一各血液样本容器中血液样本进行搅拌作业时造成污染。具体地，在清洗部件113完成对一个血液样本容器91中的血液样本的搅拌作业时，通过清洗液进口1131输入清洗液对样本搅拌部件112进行清洗，并将清洗后的清洗液通过清洗液排出口1132排出，回收清洗液。同时，在本实施方式中，在用清洗液对样本搅拌部件112进行清洗后，还可以对样本搅拌部件112进行干燥处理，例如通过清洗液排出口1132进行抽气，以此风干样本搅拌部件112。直接利用清洗液排出口1132进行抽气风干，可以节约风干设备，使得装置小型化。
在本实施方式中，通过驱动第一混匀装置11的样本搅拌部件112对样本容器中样本进行混匀作业，使得样本容器91中的全血样本中各层成份能够迅速地混匀。而且，在混匀过程中，样本容器91的底部不超过管口，并且分析仪1的采样针不接触样本容器91中的血样，并且混匀装置11能在不导致血样泼洒和血样不沾管帽前提下实现微量样本的混匀。
在本实施方式中，在使用第一混匀装置11对样本容器91中的血样进行搅拌之前需要打开样本容器91的密封盖子，或者采用无密封盖子的样本容器91。
优选地，所述样本容器91中装有微量血液样本，通常大于等于50μL而小于等于250μL，例如100μL；所述样本容器中装的样本可以是全血样本，也可以是预稀释样本；所述样本容器91中装的样本可以是微量的末梢血，也可以是微量的静脉血，只要体积小于等于250μl都适合用该第一混匀装置11进行混匀，特别适合与体积大于等于50μL而小于等于200μL以下的微量全血液样本的混匀。
图6为本实施方式的第二混匀装置的结构示意图。第二混匀装置12可抓取放置在样本架80上的被运样装置17送至分析仪1的预定位置上的常量血液样本容器并对其中的样本以颠倒混匀方式进行混匀。所述常量血液样本可以是静脉采血管92，也可以是别的类型静脉采血管；所述常量血液样本容器中装有明显大于第一样本量(微量血液样本)的第二样本量(常量血液样本)的样本，所述第二样本量通常≥1mL；所述常量血液样本容器中装的样本为静脉全血样本。
如图6所示，第二混匀装置12包括：夹爪1201，第一支撑架1211，第二支撑架1212，第三支撑架1213，步进电机1221～1223，直线滑轨1231～1232，绕在同步轮上的环形同步齿形带1241～1243，位置传感器1121～1125，传感器感应片1261～1263，转轴1271。
第一支撑架1211是第二混匀装置12的主支撑件，用于固定步进电机1221，直线滑轨1231及位置传感器1251～1252，并且第一支撑架1211通过螺钉固定于分析仪1的前板；直线滑轨1231沿Z1和Z2方向放置，第二支撑架1212以及传感器感应片1261与直线滑轨1231的滑块连接，可以沿Z1或Z2方向滑动；第二支撑架用于固定步进电机1222，直线滑轨1232，及位置传感器1253～1254；直线滑轨1232沿Y1和Y2方向放置，第三支撑架1213及传感器感应片1262与直线滑轨1232的滑块连接，可以沿Y1或Y2方向 滑动；第三支撑架1213用于固定步进电机1223，位置传感器1255，并且转轴1271以转动连接方式固定在第三支撑架1213上，转轴1271可沿R7或R8方向转动；夹爪1201及传感器感应片1263与转轴1271固定连接，可跟随转轴1271沿R7或R8方向转动。
环形同步齿形带1241受步进电机1221的旋转驱动，在两个同步轮的引导下转动。第二支撑架1212与环形同步齿形带1241连接，在步进电机1221驱动下，第二支撑架1212可带动夹爪1201和传感器感应片1261沿Z1或Z2方向移动；位置传感器1251和1252，配合传感器感应片1261，用于实现夹爪1201沿Z1或Z2方向的定位，当第二支撑架1212带动夹爪1201沿Z1运动时，采用位置传感器1252定位，当第二支撑架1212带动夹爪1201沿Z2运动时，采用位置传感器1251定位。
环形同步齿形带1242受步进电机1222的旋转驱动，在两个同步轮的引导下转动。第三支撑架1213与环形同步齿形带1242连接，在步进电机1222驱动下，第三支撑架1213可带动夹爪1201和传感器感应片1262沿Y1或Y2方向移动；位置传感器1253和1254，配合传感器感应片1262，用于实现夹爪1201沿Y1或Y2方向的定位，当第三支撑架1213带动夹爪1201沿Y1运动时，采用位置传感器1254定位，当第三支撑架1213带动夹爪1201沿Y2运动时，采用位置传感器1253定位。
环形同步齿形带1243受步进电机1223的旋转驱动，在两个同步轮的引导下转动。转轴1271在在步进电机1223驱动下，带动夹爪1201沿R7或R8方向转动；位置传感器1255配合传感器感应片1263实现夹爪沿R8方向运动时的定位。
图7为本实施方式的吸样装置的结构示意图。如图7所示，吸样装置13用于从运样装置17送至分析仪1采样位的样本容器91(92)中吸取血样用于制样。
如图7所示，吸样装置13包括：吸样针135、吸样针移动组件131、步进电机1301、同步轮1302和1303、绕在同步轮1302和1303上的环形同步齿形带1304、沿Y1和Y2方向放置的直线导杆1305、位置传感器1306等。
吸样针移动组件131通过连接件与环形同步齿形带1304连接。环形同步齿形带1304受步进电机1301的旋转驱动，在两个同步轮1302和1303的引导下转动。吸样针移动组件131可以在步进电机1301的驱动下带动吸样针135沿Y1或Y2方向移动。吸样针移动组件131的在Y1、Y2方向的初始位置通过位置传感器1306和固定在吸样针移动组件131的传感器感应片1318实现定位。
吸样针移动组件131包括：步进电机1311、螺杆1312、螺母1323、直线滑轨1314、吸样针固定部件1315、位置传感器1316、以及传感器感应片1317等。
吸样针135固定在吸样针固定部件1315上，吸样针固定部件1315通过螺钉固定在沿Z1、Z2放置的直线滑轨1314上，同时螺母1313被卡在吸样针固定部件1315设置的卡槽中，并且螺母1313相和吸样针固定部件1315之间不产生相对转动。丝杠1312通过螺钉与步进电机1311转轴连接。步进电机1311可以带动螺杆1312转动，并驱动吸样针固定部件1315带着吸样针135沿Z1或Z2方向移动。吸样针135在Z1、Z2方向的初始位置通过位置传感器1317和设置在吸样针固定部件1315上的光耦传感器感应片(无图示)实现定位。
在步进电机步进电机1301和步进电机1311驱动下，吸样针135可以沿Y1、Y2方向以及Z1、Z2方向做二维移动。可以实现从样本容器中吸取血样，以及到制样器中分血样的功能。
图8～11为本实施方式的容器旋转扫码装置结构示意图；图12～13为本实施方式的容器旋转扫码装置工作原理示意图。
容器旋转扫码装置14～16(其中14是容器压紧组件，15是容器旋转组件，16是扫码器)用于获取运样装置17送至分析仪1扫码位的样本容器标签上读取样本的编码信息，用于分析仪的样本信息管理。
如图8所示，容器压紧组件14包括：步进电机141、从动轮支架142、直线滑轨143、 两个从动轮144a和144b。从动轮144a和144b可转动的固定在从动轮支架142，从动轮支架142固定在直线滑轨143的滑块上。直线滑轨143沿Y1、Y2方向设置，在步进电机141驱动下，从动轮支架142可以带着从动轮144a和144b沿Y1、Y2方向运动。此外，从动轮支架142设置了缺口1421用于避让扫码器16的扫码视窗。
如图9所示，容器旋转组件15包括：步进电机151、旋转轮152、橡胶垫153、联轴器154。旋转轮152通过联轴器154与步进电机151的转轴相连，在步进电机151驱动下，旋转轮152可以逆时针或顺时针旋转。套在旋转轮152外圈的橡胶垫153是为了增大旋转轮152与容器间的摩擦力。
如图10所示，在容器压紧组件14的初始位置，在步进电机141驱动下，从动轮支架142可以带着从动轮144a和144b沿Y1方向运动，将样本容器挤向容器旋转组件15的旋转轮152(参照图11)。此时，若旋转组件15的旋转轮152在步进电机151驱动下绕其中心O1点沿R11方向旋转，在摩擦力作用下，样本容器、滚轮144a和144b分别绕各自中心轴O2、O3和O4沿R12、R13和R14旋转；或者步进电机151驱动旋转轮152绕其中心O1点沿R11’方向旋转，则样本容器、滚轮144a和144b分别绕各自中心轴O2、O3和O4沿R12’、R13’和R14’旋转(参照图12)。样本容器在旋转过程中，粘贴在样本容器上的条码标签将有一个阶段朝向条码读取装置16，此时扫码器16就能读取样本容器上的条码标签的编号信息(参照图13)。
在本实施方式中，容器旋转扫码装置可以支持对这样具有合适高度和内径的、能放入试管架的、管壁上能贴条码的样本容器进行扫码，优选细长型的样本容器。
图14为本实施方式的样本架结构示意图。如图14所示，样本架80设置有固定样本容器用的固定孔801a，每个固定孔801a对应设有开口801b，开口801b作为样本容器条码标签的扫码窗口；另外，样本架80还专门设有样本架标签粘贴区802，样本架标签粘贴区802可粘贴条形码标签，二维码标签，或RFID标签等标签。优选地，样本架80标签的编码信息中包含测量模式信息。
样本架80可直接固定若干静脉采血管92或微量采血管91(参见图15)，或者可以通过适配器81固定微量采血管91(参见图16)。
适配器81(参照图17)设置了可固定微量采血管91的固定孔811，还有台阶812，可以防止适配器81在夹爪1201上升过程中掉落，以及为减轻适配器的重量而将底部设置了空腔814。适配器81比适配器82多了一个可阻挡微量采血管91的管帽的限制部813，在将微量采血管91放入适配器81的固定孔811时，需要将微量采血管91的连接部913卡入适配器81的的限制部813。由于微量采血管91的管帽和管身不可分离，为了避免管帽在连接部的回复力作用下盖向管身，通过适配器81的限制部813限制管帽，防止在采样针在进入微量采血管91吸样时扎在管帽上。优选地，为了避免在适配器81搅拌混匀时微量采血管91的连接部脱出适配器81的的限制部813，将限制部813设置成了Z字形状。适配器81的外壁直径小于样本架80的样本容器固定孔801a的孔径，适配器81的固定孔811内径略大于固定微量采血管91管身的外径；适配器81的外壁直径可以与适配器82的外壁直径相等。
适配器82(参照图18)设置有用于固定微量采血管91的固定孔821，还有台阶822(其功能与适配器81的台阶812相同)；此外适配器82为了减轻重量在其底部设有空腔823。适配器82的外壁直径小于样本架80的固定孔801a的孔径，适配器82的固定孔821内径略大于固定微量采血管91管身的外径。
上述微量采血管91，不管是通过毛细管还是通过刮血方式采末梢血，能够采集到的血样都较少(绝大多数情况≤100μL，少数情况会采到200-250μL)。
在本实施方式中，所述样本容器91可以是微量采血管中的一种或几种，也可以是别的类型微量管；所述微量样本的样本量通常≤250μL，优选地为30～250μL，更优选地为50～200μL，更更优选地为50～100μL；所述微量样本可以是全血样本，也可以是 预稀释样本；所述微量样本可以是微量的末梢血，也可以是微量的静脉血。
图19为控制装置21的结构框图。如图19所示，控制装置21主要由CPU211a、ROM211b、RAM211c、硬盘211d、读取装置211e、输入输出接口211f、通信接口211g和图像输出接口211h构成。CPU211a、ROM211b、RAM211c、硬盘211d、读取装置211e、输入输出接口211f、通信接口211g和图像输出接口211h通过总线211i连接。
CPU211a能够执行存储在ROM211b的计算机程序和下载到RAM211c的计算机程序。CPU211a执行后述应用程序214a、214b和214c，由此发挥控制装置21的功能。
ROM211b由掩膜ROM、PROM、EPROM、EEPROM等构成，其中存储着由CPU211a执行的计算机程序及其所需要的数据等。
RAM211c由SRAM或DRAM等构成。RAM211c用于读取ROM211b和硬盘211d中存储的计算机程序。RAM211c还可以用作CPU211a执行这些计算机程序时的工作空间。
硬盘211d装有操作系统和应用程序等供CPU211a执行的各种计算机程序及执行该计算机程序时所用的数据。第一混匀装置11用的第一混匀处理程序214a、第二混匀装置12用的第二混匀处理程序214b、以及运样装置17用的样本运送作业处理程序214c也装在此硬盘211d中。CPU211a执行这些应用程序214a～214c，由此来控制第一混匀装置11、第二混匀装置12和运样装置17的各部分的作业。
读取装置211e由软盘驱动器、CD-ROM驱动器或DVD-ROM驱动器等构成，能够读取便携型存储介质214中存储的计算机程序或数据。便携型存储介质214中存储有应用程序214a～214c，控制装置21能够从该便携型存储介质214读取应用程序214a～214c，并能够将这些应用程序214a～214c装入硬盘211d。
上述应用程序214a～214c不仅能够由便携型存储介质214提供，还可以从通过电子通信线路(不论有线、无线)与控制装置21进行了可通信连接的外部机器上经由该电子通信线路提供上述程序。
硬盘211d中装有可提供图形用户界面的操作系统。在以下说明中，应用程序214a～214c均在上述操作系统上运行。
输入输出接口211f由串行接口、并行接口、以及包括D/A转换器、A/D转换器等的模拟接口。输入输出接口211f连接着输入设备23，用户能够用该输入设备23向控制装置21输入数据。
通信接口211g是有线或无线的通信接口。控制装置21通过该通信接口211g就能使用一定的通信协议与第一混匀装置11、第二混匀装置12和运样装置17传输数据。
图像输出接口211h连接着由LCD或CRT等构成的显示部件31，将与从CPU211a接收的图像数据相应的影像信号输出到显示部件31。显示部件31按照输入的影像信号显示图像(界面)。
控制装置21通过上述结构控制第一混匀装置11、第二混匀装置12和运样装置17的作业。
图20和图21为血样分析仪1分析处理血液样本一例的主要流程框图。如图20和图21所示，首先接通血样分析仪1的电源，则控制装置21开始初始化(步骤S1)。在此初始化步骤中，进行程序的初始化和血样分析仪1的液路器部件初始化、管路的清洗和驱动部分复位等操作。
接着在步骤S2，控制装置21判断是否需要进行样本架类型设置。如果需要进行样本架类型设置(步骤S2：是)，则进入步骤S3，如果判断无需要进行样本架类型设置(步骤S2：否)，则进入步骤S5。
接着，在步骤S3，显示部件31显示样本架类型设置界面(参照图22)，用户进入样本架类型设置界面设置样本架信息。在该界面第一列可以设定样本架80的编号，第二列可以将对应编号的样本架80设定为微量全血样本架或预稀释血液样本架，二者最多只能选其一。如果没有勾选微量全血样本架或预稀释血液样本架，或者没有在该界面设定 编号的样本架80，血样分析仪1均视为常量血液样本架处理。即，当勾选微量全血样本架或预稀释血液样本架，则对应编号的样本架80上的样本容器，血样分析仪1全当做第一样本容器91处理；没有勾选微量全血样本架或预稀释血液样本架，或者没有在该界面设定编号的样本架80上的样本容器，血样分析仪1全当做第二样本容器92处理。
当第二列勾选的是微量全血(第一测量模式)时，血液分析仪1的吸样针135从对应编号的样本架80上的第一样本容器91中吸样时，吸移第一采样量的血液样本，例如优选5-50μL，更优选15-35μL，最常用的是30μL；当第二列勾选的是预稀释(第三测量模式)时，血液分析仪1的吸样针从对应编号的样本架80上的第一样本容器91中吸样时，吸移第三采样量的血液样本，例如80μL；当没有勾选微量全血样本架或预稀释血液样本架(第二测量模式)时，血液分析仪1的吸样针从对应编号的样本架80上的第二样本容器92中吸样时，吸移第二采样量的血液样本，例如50-300μL，最常用的70μL。优选的，第一采样量小于第二采样量。
图22为血液分析仪1的设置界面。如图22所示，用户可以通过显示部件31调用设置界面，将某些编号的样本架设为微量采血管专用样本架。凡在图22所示界面输入了编号的样本架，血液分析仪1均将视为作微量采血管专用样本架处理，在混匀时通过搬运装置将固定于微量采血管专用样本架上的样本容器91搬运至第一混匀装置11进行混匀(后面详细阐述)。凡在图22所示界面没有输入编号的样本架，血液分析仪1均将视为普通静脉采血管样本架处理，混匀时采用第二混匀装置12抓取该样本架上的样本容器92进行混匀。
在如图22所示的分析仪设置界面，用户还可以设置微量采血管专用样本架所固定的微量采血管的类型(参照图22第三列，在软件中以预先设定最常见的若干微量管类型供选择)，以及该微量采血管中装的样本量(参照图21第四列)，血液分析仪1的控制装置21根据用户的设置自动选择第一混匀装置11的用于驱动样本搅拌部件112的电机111的转速，其中微量采血管类型、尺寸以及样本量与电机转速的关联关系，预先在软件程序中已设定好。
在如图22所示的血液分析仪设置界面，如果用户将某些编号的样本架设为微量采血管专用样本架，但没有对应的设置微量采血管类型或样本量，血液分析仪1的控制装置21将第一混匀装置11的用于驱动样本搅拌部件112的电机111的转速设为默认转速用于该编号样本架上的样本容器的混匀。
如图22所示的设置，对于编号为1～5的样本架，血液分析仪1将使用第一混匀装置11对该样本架上的样本容器进行混匀，对编号不是1～5的样本架，分析仪1将使用第二混匀装置12对该样本架上的样本容器进行混匀。
在一个实施方式中，可以进一步对编号1-5的样本架区分，在编号1-3，编号4和5样本架上分别放置不同体积血样的样本容器，或放置不同形状或尺寸的样本容器，对于编号为1～3的样本架上的样本容器，第一混匀装置11的电机111采用M1圈/转的转速进行混匀；对于编号为4的样本架上的样本容器，第一混匀装置11的电机111以默认转速M0圈/转的转速进行混匀；对于编号为5的样本架上的样本容器，第一混匀装置11的电机111采用M2圈/转的转速进行混匀。
血液分析仪1将编号为1～5的样本架80上的样本容器全当作第一样本容器91处理，而将编号非1～5的样本架上的样本容器全当作第二样本容器94处理。血液分析仪1的吸样针从编号为1～4的样本架上的样本容器91吸样时，吸移第一采样量的血液样本(微量全血样本)；血液分析仪1的吸样针从编号为5的样本架80上的样本容器91吸样时，吸移第三采样量的血液样本(预稀释血液样本)；血液分析仪1的吸样针从编号为非1～5的样本架80上的样本容器92吸样时，吸移第二采样量的血液样本(常量血液样本)。
返回图20，在步骤S4中存储样本架信息，然后进入步骤S5。在步骤S5中，用户选择进样模式。
在步骤S6，控制装置21判断是否为第一进样模式。如果判断为第一进样模式(步骤S6：是)，则控制装置21判断是否按下启动按钮(未图示)(步骤S7)。如果控制装置21判断未按下启动按钮(步骤S7：否)，则进入步骤S25。如果判断启动按钮已按下(步骤S7：是)，则进入步骤S8。
在步骤S8，运样装置17将样本架80上的样本容器91(92)逐一运送至扫码位(无图示)，由容器旋转扫码装置(包括容器压紧组件14、容器旋转组件15、扫码器16)读取样本容器91(92)标签911(921)上的样本编码信息，以及对经过扫码位的样本架80进行扫码，读取样本架80标签的编码信息(步骤S9)。
控制装置21控制运样装置17将样本架80上的样本容器91(92)逐一运送至预定位置22(步骤S10)。
控制装置21控制第一混匀装置11或第二混匀装置12对样本容器91(92)中的血液样本进行混匀作业(步骤S11)，在步骤S11中，控制装置21基于从样本架80标签读取的编码信息判断当前的测量模式是第一测量模式、第二测量模式或第三测量模式，如果控制装置21判断当前的测量模式是第一测量模式或第三测量模式，移动第一混匀装置11对位于上述预定位置22(第一混匀位)的样本架80上的样本容器91中的血液样本进行搅拌混匀作业；如果控制装置21判断当前的测量模式是第二测量模式，控制第二混匀装置12的夹爪1201从位于上述预定位置的样本架80上抓取当前样本容器92到一定位置(第二混匀位)(无图示)，并控制第二混匀装置12的步进电机1223驱动夹爪1201转动，从而对当前样本容器进行血液样本的混匀作业。在其他实施方式中，第一混匀位可以设置在样本架上，即样本架上的孔位作为第一混匀位，搅拌部移动到此位置对位于第一混匀位中的试管中的样本进行混匀；或者第一混匀位为相对样本架单独设置的固定位，便于对试管在搅拌作业时进行更好的固定。当第一混匀位为相对样本架单独设置的固定位时，可以额外设置搬运装置抓取第一样本容器运送到第一混匀位，也可以利用第二混匀装置的抓取机构作为搬运装置运送第一样本容器。当第一混匀位可以设置在样本架上时，优选地，如图23所示，控制装置21控制容器压紧组件14移动使得两个从动轮144a和144b夹紧样本架80上的样本容器91(92)，并控制第一混匀装置11的样本搅拌部件112向下(Z方向)进入样本容器91(92)中的血液样本进行混匀作业。
在本实施方式中，在步骤S11中，也可以由第二混匀装置12的夹爪1201从上述预定位置22的样本架80上抓取样本容器91(92)并移至上述一定位置，然后移动第一混匀装置11使得第一混匀装置11的样本搅拌部件112进入夹爪1201所抓取的样本容器91进行搅拌混匀动作。
在本实施方式中，优选地，如图23所示，在步骤S11中，当控制装置21根据样本架80标签的编码信息确定当前样本架80上的样本容器是装有微量全血样本(或预稀释微量血液样本)的样本容器91时，控制容器压紧组件14移动使得两个从动轮144a和144b夹紧样本架80上的样本容器91，并控制第一混匀装置11的样本搅拌部件112向下(Z方向)进入样本容器91中的血液样本进行混匀作业；当控制装置21根据样本架80标签的编码信息确定当前样本架80上的样本容器是装有常量血样本的样本容器92时，控制第二混匀装置12驱动夹爪1201从样本架80上抓取样本容器92至一定位置并进行转动，以对样本容器92中的血液样本进行混匀作业(例如进行颠倒混匀)。
在本实施方式中，也可以由控制装置21控制容器压紧组件14移动使得两个从动轮144a和144b夹紧样本架80上的样本容器91并移至一定位置，并控制第一混匀装置11的样本搅拌部件112向下(Z方向)进入样本容器91中的血液样本进行混匀作业。
将装有混匀后的血液样本的样本容器91(92)运送至血样分析仪的第一采样位(无图示)(步骤S12)，然后进入步骤S13。
在步骤S13中，控制装置21根据收到的测量模式信息控制吸样装置13的吸样针135从采样位上的样本容器91(92)中吸移预定量的血液样本。具体地，在第一测量模式下， 吸样装置13的吸样针135从第一样本容器91中吸移第一采样量的血液样本；在第三测量模式下，吸样装置13的吸样针135从第一样本容器91中吸移第三采样量的血液样本；在第二测量模式下，吸样装置13的吸样针135从第二样本容器92中吸移第二采样量的血液样本。
在步骤S14中，血样分析仪1的制样器将吸样装置13吸移的血液样本制备检测用试样。其中，在第一测量模式下，用所吸移的第一采样量的血液样本制备第一检测用试样；在第三测量模式下，用所吸移第三采样量的预稀释血液样本制备第三检测用试样；在第二测量模式下，用所吸移第二采样量的血液样本制备第二检测用试样。
在步骤S15中，血样分析仪1的检测器对制样器所制备的检测用试样进行检测，获得检测结果。控制装置21判断样本架上是否存在未处理的下一个样本容器91(92)(步骤S16)，如果还有未处理的样本容器91(92)(步骤S16：是)，返回步骤S8进行相应处理。如果已经处理完所有的样本容器91(92)(步骤S16：否)，结束第一进样模式(步骤S17)，进入步骤S26。
如果判断为第二进样模式(步骤S6：否)，则打开样本仓盖181(步骤S18)。关于步骤S18，当控制装置21为第二进样模式时，如果样本仓盖181原先为关闭状态，则执行本步骤S18，如果样本仓盖181原先为打开状态，则直接进入下一步骤S19。
在步骤S19中，用户通过血液分析仪1的设置界面选择当前血液样本的测量模式。
控制装置21判断是否按下启动按钮(未图示)(步骤S20)。如果控制装置21判断未按下启动按钮(步骤S20：否)，则进入步骤S26。如果判断启动按钮已按下(步骤S20：是)，则进入步骤S21，关闭样本仓盖181，进入步骤S22。
在步骤S22中，控制装置21根据步骤S19中用户所选择的测量模式信息控制吸样装置13的吸样针135从第二采样位上的样本容器91(92)中吸移预定量的血液样本。具体地，在第一测量模式下，吸样装置13的吸样针135从第一样本容器91中吸移第一采样量的血液样本；在第三测量模式下，吸样装置13的吸样针135从第一样本容器91中吸移第三采样量的预稀释血液样本；在第二测量模式下，吸样装置13的吸样针135从第二样本容器92中吸移第二采样量的血液样本。优选的，第一采样量小于第二采样量，例如第一采样量为5-50μL，更优选的为15-35μL。
在步骤S23中，血样分析仪1的制样器将吸样装置13吸移的血液样本制备检测用试样。其中，在第一测量模式下，用所吸移的第一采样量的血液样本制备第一检测用试样；在第三测量模式下，用所吸移第三采样量的预稀释血液样本制备第三检测用试样；在第二测量模式下，用所吸移第二采样量的血液样本制备第二检测用试样。
在步骤S24中，血样分析仪1的检测器对制样器所制备的检测用试样进行检测，获得检测结果，结束第二进样模式(步骤S25)，进入步骤S26。
在步骤S26，如果未接收到关机指示(步骤S26：否)，返回步骤S2；如果接收到关机指示(步骤S26：是)，执行关机(步骤S27)，结束处理。
在上述实施方式中，可以用于对血常规中涉及红细胞、白细胞和血小板等主要检查项目进行血液分析，并在步骤S15中，通过检测器对制样器所制备的检测用试样进行检测，获得涉及红细胞、白细胞和血小板等主要检查项目的相关指标，比如白细胞计数(WBC)、红细胞计数(RBC)、血红蛋白浓度(HGB)、红细胞压积(HCT)、平均红细胞体积(MCV)、平均红细胞血红蛋白含量(MCH)、平均红细胞血红蛋白浓度(MVHC)、血小板计数(PLT)、淋巴细胞比值(LY％)、单核细胞比例(MONO)、中性粒细胞比例(NEUT％)、淋巴细胞(LY)、单核细胞计数(MONO)、中性粒细胞计数(NEUT)、红细胞分布宽度(RDW)、血小板体积分布宽度(PDW)、平均血小板体积(MPV)和/或大血小板比例(P-LCR)等等指标。
图24显示了步骤S11中混匀作业的流程示意框图。如图24所示，控制装置21将从样本架80标签读取的编码信息与用户预设样本架信息比对(步骤S1101)，并判断所读 取的编码信息与预设样本架信息是否匹配(步骤S1102)。如果所读取的编码信息与预设样本架信息匹配(步骤S1102：是)，则判断当前样本架上的样本执行第一或第三测量模式，调用预设混匀参数设置第一混匀装置11(步骤S1103)，然后控制装置21控制第一混匀装置11对用于第一测量模式或第三测量模式的当前样本容器91进行血液样本的混匀作业(步骤S1104)。如果所读取的编码信息与预设样本架信息不匹配(步骤S1102：否)，则判断当前样本架上的样本执行第二测量模式，控制装置21控制第二混匀装置12对当前样本容器92进行血液样本的混匀作业(步骤S1105)。
在本实施方式的步骤S1104中，第一混匀装置11驱动样本搅拌部件112对样本容器91中的血液样本完成混匀作业后，对样本搅拌部件112进行清洗并干燥。优选地，可以采用清洗拭子对样本搅拌部件112进行清洗和风干(参照图5)。此实施方式中，可以是拭子固定，移动样本搅拌部件112的方式清洗和风干，也可以固定样本搅拌部件112，移动拭子的方式进行清洗和风干。
在本实施方式中，清洗部件还可以是包括清洗池114，在步骤S1104中，还可以通过一个清洗池进行清洗(参照图25)。即，样本搅拌部件112完成搅拌后，自行移动到清洗池114内，通过清洗池114内液体冲刷等方式，将样本搅拌部件112清洗干净。
图26为本实施方式中第一进样模式下的步骤S13吸移样本流程例示图。如图26所示，将容器旋转扫码装置读取的样本架80标签的编码信息与用户预设的样本架信息进行比对(步骤S131)。基于比对结果判断当前样本架80是否为微量全血样本架(步骤S132)，如果是微量全血样本架(步骤S132：是)，将装置的测量模式设定为第一测量模式(步骤S133)，然后吸样装置13从该样本架80上的样本容器91吸移第一采样量的血液样本(步骤S134)。
如果不是微量全血样本架(步骤S132：否)，则判断是否为预稀释微量血样本架(步骤S135)。如果是预稀释微量血样本架(步骤S135：是)，将装置的测量模式设定为第三测量模式(步骤S136)，然后吸样装置13从该样本架80上的样本容器91吸移第三采样量的血液样本(步骤S137)。
如果不是预稀释微量血样本架(步骤S135：否)，将装置的测量模式设定为第二测量模式(步骤S138)，然后吸样装置13从该样本架80上的样本容器92吸移第二采样量的血液样本(步骤S139)。
图27为本实施方式中第二进样模式下的步骤S22吸移样本流程例示图。如图27所示，判断用户选设的测量模式是否为第一测量模式(步骤S221)，如果是第一测量模式(步骤S221：是)，吸样装置13从该样本架80上的样本容器91(92、93)吸移第一采样量的血液样本(步骤S222)。如果不是第一测量模式(步骤S221：否)，则判断是否为第二测量模式(步骤S223)。如果是第二测量模式(步骤S223：是)，吸样装置13从该样本架80上的样本容器94吸移第二采样量的血液样本(步骤S224)。如果不是第二测量模式(步骤S223：否)，则吸样装置13从该样本架80上的样本容器91(92、93)吸移第三采样量的血液样本(步骤S225)。
(实施方式2)
在本实施方式中的血液分析仪的结构与实施方式1的血液分析仪1的区别在于：本实施方式中的血液分析仪没有第二混匀装置12，其余部分与实施方式1的血液分析仪1中相应部分的结构相同，因此相同结构部分使用同样的标号并省略说明。
图28和图29显示了血样分析仪分析处理血液样本一例的主要流程。步骤S421～S430、S432～S447与实施方式1中的步骤S1～S10、S12～S27的处理作业相同，在此故省略其说明。
在步骤S431中，控制装置21控制第一混匀装置11移动至用于第一测量模式或第三测量模式的当前样本容器91上方后下降第一混匀装置11的样本搅拌部件112，并驱动样本搅拌部件112对样本容器91中的血液样本进行混匀作业。
优选地，如图23所示，控制装置21控制容器压紧组件14移动使得两个从动轮144a和144b夹紧样本架80上的样本容器91(92)，并控制第一混匀装置11的样本搅拌部件112向下(Z方向)进入样本容器91(92)中的血液样本进行混匀作业。
在本实施方式中，血液分析仪只有第一混匀装置11。该第一混匀装置11可以用于混微量全血样本、预稀释血液样本或静脉血样本，在运样装置17运送样本架80之前将样本架80上的样本容器92的容器盖打开。优选地，该第一混匀装置11仅用于混微量全血样本和预稀释血液样本。
(实施方式3)
在本实施方式中的血液分析仪的结构与实施方式2的血液分析仪的区别在于：本实施方式中的血液分析仪仅设置了第二进样模式，而不设置运样装置17，即不设置第一进样模式，从而使得血液分析仪更加小型化。其余部分与实施方式2的血液分析仪中相应部分的结构相同，因此相同结构部分使用同样的标号并省略说明。
图30为血样分析仪1分析血液样本一例的主要流程框图。如图30所示，首先接通血样分析仪1的电源，则控制装置21开始初始化(步骤S501)。在此初始化步骤中，进行程序的初始化和血样分析仪1的液路器部件初始化、管路的清洗和驱动部分复位等操作。
接着在步骤S502，在显示部件31显示的设置界面进行测量模式的选择。控制装置21判断是否按下启动按钮(未图示)(步骤S503)。如果控制装置21判断未按下启动按钮(步骤S503：否)，则进入步骤S510。如果判断启动按钮已按下(步骤S503：是)，则关闭样本仓盖181(步骤S504)。关于步骤S504，当样本仓盖181原先为关闭状态时，则直接进入下一步骤S505，如果样本仓盖181原先为打开状态，则执行本步骤S504。
控制装置21控制第一混匀装置11或第二混匀装置12对样本容器91(92)中的血液样本进行混匀作业(步骤S505)。在步骤S505中，控制装置21判断当前的测量模式是第一测量模式、第二测量模式或第三测量模式，如果控制装置21判断当前的测量模式是第一测量模式或第三测量模式，控制第一混匀装置11对样本容器固定孔182上的样本容器91进行血液样本的混匀作业。
在步骤S505中，如果控制装置21判断当前的测量模式是第二测量模式，控制第二混匀装置12的步进电机1223驱动夹爪1201从样本容器固定孔18中抓取样本容器92至一定位置并进行转动，从而对当前样本容器92进行血液样本的混匀作业。本领域技术人员能够理解，也可以不设第二混匀装置，仅检测微量全血或预稀释的血液样本，进一步使血液分析仪小型化。
在步骤S506中，控制装置21根据用户选择的测量模式信息控制吸样装置13的吸样针135从采样位上的样本容器91(92)中吸移预定量的血液样本。具体地，在第一测量模式下，吸样装置13的吸样针135从第一样本容器91中吸移第一采样量的血液样本；在第三测量模式下，吸样装置13的吸样针135从第一样本容器91中吸移第三采样量的血液样本；在第二测量模式下，吸样装置13的吸样针135从第二样本容器92中吸移第二采样量的血液样本。优选的，第一测量模式下的第一采样量少于第二模式下的第二采样量，例如第一采样量优选为5-50μL，更优选为15-35μL。
完成血液样本吸移处理后，打开样本仓盖181，取出已吸移的样本容器91(92)(步骤S507)。在本申请中，打开样本仓盖181取出已吸移的样本容器91(92)的步骤，也可以在完成血液样本吸移处理后的任何时候进行操作，并不限于完成血液样本吸移处理后立即取出。
在步骤S508中，血样分析仪1的制样器将吸样装置13吸移的血液样本制备检测用试样。其中，在第一测量模式下，用所吸移的第一采样量的血液样本制备第一检测用试样；在第三测量模式下，用所吸移第三采样量的预稀释血液样本制备第三检测用试样；在第二测量模式下，用所吸移第二采样量的血液样本制备第二检测用试样。优选的，第 一采样量小于第二采样量，例如第一采样量为5-50μL，更优选的为15-35μL。
在步骤S509中，血样分析仪1的检测器对制样器所制备的检测用试样进行检测，获得检测结果，进入步骤S510。
在步骤S510，如果未接收到关机指示(步骤S510：否)，返回步骤S502；如果接收到关机指示(步骤S510：是)，执行关机(步骤S511)，结束处理。
在本实施方式中，由第一混匀装置11进行混匀的样本容器91中的样本可以是全血样本，也可以是预稀释样本。所述全血样本可以是末梢全血样本，也可以是静脉全血样本。
在本实施方式中，优选地，由第一混匀装置11仅针对微量全血样本或预稀释血液样本进行混匀作业。
血液由血细胞和血浆构成，由于血细胞的比重大于血浆的比重，血液在静置一段时间后会发生分层，其中血细胞沉于下方而血浆位于上方。血样测量有一项参数是血红蛋白浓度(HGB)，它是指单位体积血液内所含血红蛋白的量。血红蛋白又称血色素，仅存在于红细胞中，是红细胞的主要组成部分。
当血样未充分混匀时，血样下部分的红细胞浓度高于上部分，当采样针在靠近采血管底部吸取样本时(血液分析仪为了降低血液分析仪对采血量的要求，采样针都会在接近采血管底部吸样)，血液分析仪测得的血红蛋白浓度(HGB)会明显高于实际值，并且多次测量血红蛋白浓度(HGB)的波动幅度较大。因此，血红蛋白浓度(HGB)测量的稳定性常常用来衡量血样混匀的效果。
在上述各实施方式中，不但避免了血液黏附在采血管帽或管壁上造成血样损失及末梢血混匀问题，而且能够充分搅拌均匀，当血样充分混匀时，其血红蛋白浓度(HGB)是一个非常稳定的参数，多次重复测量的波动幅度一般不超过±2g/L。
在上述实施方式中，通过混匀装置驱动电机驱动驱动第一混匀装置11的样本搅拌部件112进入样本容器进行搅拌作业。本申请并不限于此，还可以运样装置17将装有样本容器的样本架80运送至预定位置，通过第二混匀装置12的夹爪1201抓取和移动样本架80或样本架80上的样本容器至混匀位，从而在不移动第一混匀装置11的情况下，使得样本搅拌部件112相对的进入样本容器，并通过驱动样本搅拌部件112对样本容器中的血液样本进行搅拌动作。以此对样本容器中的血液样本进行搅拌作业，从而可以省去第一混匀装置11的移动装置。
在上述实施方式中，也可以将样本搅拌部件112安装在一个可以上下移动的机构上，通过电机驱动，皮带轮或者丝杆传动，控制搅拌杆上下移动。同时，样本搅拌部件112安装在上下移动机构的方式通过轴承连接，使得样本搅拌部件112在上下移动的同时可以沿样本搅拌部件112自身轴线做自转运动。上述样本容器91(92)放置在样本架80上，通过运样装置17水平运动至预定位置(混匀位)。样本容器91(92)到达后，样本搅拌部件112向下移动(如图23中的Z方向)，伸入样本容器91(92)内并到达样本容器91(92)底部。样本搅拌部件112到位后，通过沿样本搅拌部件112自身轴线自转的方式，带动样本容器91(92)内部的样本旋转，达到样本混匀的效果。完成混匀后，样本搅拌部件112上升同时，通过清洗部件113清洗样本搅拌部件112外壁粘附的少量样本。当样本搅拌部件112离开样本容器91(92)后，运样装置17推进样本容器91(92)运动，使得样本容器91(92)到达采血位，分析仪开始采血并分析。
本申请可用于测量血红蛋白浓度(HGB)。血红蛋白浓度(HGB)是血样测量的一项重要参数，它是指单位体积血液内所含血红蛋白的量。血红蛋白又称血色素，仅存在于红细胞中，是红细胞的主要组成部分。血液由血细胞和血浆构成，由于血细胞的比重大于血浆的比重，血液在静置一段时间后会发生分层，其中血细胞沉于下方而血浆位于上方。
当血样未充分混匀时，血样下部分的红细胞浓度高于上部分，当采样针在靠近采血 管底部吸取样本时(血液分析仪为了降低血液分析仪对采血量的要求，采样针都会在接近采血管底部吸样)，血液分析仪测得的血红蛋白浓度(HGB)会明显高于实际值，并且多次测量血红蛋白浓度(HGB)的波动幅度较大。因此，血红蛋白浓度(HGB)测量的稳定性常常用来衡量血样混匀的效果。
在本申请中，通过制样器将受检者的血液样本制备血红蛋白浓度(HGB)检测项目用的检测用试样，并由检测器获取血红蛋白浓度(HGB)相关指标。
在上述各实施方式中，不但避免了血液黏附在采血管帽或管壁上造成血样损失及末梢血混匀问题，而且能够充分搅拌均匀，当血样充分混匀时，其血红蛋白浓度(HGB)是一个非常稳定的参数，多次重复测量的波动幅度一般不超过±2g/L。此处的多次重复测量指两次以上的测量。
图31为6支各100μL的微量全血血样分析数据图示。由混匀装置11混匀，用第一测量模式分别测量6次的数据，从图31的数据来看，血红蛋白浓度(HGB)波动幅度只有1g/L，非常稳定。
图32是分别含有30μL、50μL、100μL、150μL、200μL和250μL的不同微量全血的第一样本容器91各6支，由混匀装置11混匀，用第一测量模式分别测量6次HGB的数据，从数据看出，血红蛋白浓度(HGB)波动幅度均不超过±2g/L，满足测量要求。
上述实施方式1中，第二混匀装置12可以抓取样本架80上的样本容器，并驱动装有常量血的样本容器进行颠倒混匀。但本申请并不限于此，第二混匀装置12也可以抓取样本架80，并驱动样本架80上装有常量血的所有样本容器进行颠倒混匀。
在本申请中，混匀位指第一混匀装置11或第二混匀装置12对样本容器中的血液样本进行混匀的位置。例如，当第一混匀装置11对位于预定位置上的样本容器中的血液样本进行混匀时，第一混匀位置与预定位置为同一位置。
(实施方式4)
图33为本实施方式的混匀装置的结构示意图。如图33所示，运样装置17或样本仓组件18将样本容器91(92)运送至采样位。所谓采样位是指采样针205(135)进行采样的位置。吸样装置13移动吸样针205进入样本容器91(92)，并驱动吸样针205吸入适量血液样本后,在将所吸入的血液样本回退至样本容器91(92)中,使得样本容器91(92)中的血液样本形成一定流动,将血液样本混匀。
图34为本实施方式的吸样装置的结构示意图。如图34所示，吸样装置20用于对运样装置17送至分析仪1采样位的样本容器91(92)的血液样本进行混匀并从混匀后的血液样本中吸取适量的血液样本用于制样。
吸样装置20包括：吸样针205、吸样针移动组件201、步进电机2001、同步轮2002和2003、绕在同步轮2002和2003上的环形同步齿形带2004、沿Y1和Y2方向放置的直线导杆2005、位置传感器2006、吸吐驱动装置(无图示)、吸样针风干装置(无图示)等。其中，吸吐驱动装置用于驱动吸样针205吸入适量血液样本后,在将所吸入的血液样本回退至样本容器91(92)中,使得样本容器91(92)中的血液样本形成一定流动,将血液样本混匀，优选地，吸吐驱动装置为注射器。
吸样针移动组件201通过连接件与环形同步齿形带2004连接。环形同步齿形带2004受步进电机2001的旋转驱动，在两个同步轮2002和2003的引导下转动。吸样针移动组件201可以在步进电机2001的驱动下带动吸样针205沿Y1或Y2方向移动。吸样针移动组件201的在Y1、Y2方向的初始位置通过位置传感器2006和固定在吸样针移动组件201的传感器感应片2018实现定位。
吸样针移动组件201包括：步进电机2011、螺杆2012、螺母2023、直线滑轨2014、吸样针固定部件2015、位置传感器2016、以及传感器感应片2017、吸样针位置传感器(无图示)等。其中，吸样针位置传感器用于感知吸样针205下行位置,防止吸样针205的针尖到达样本容器91(92)的底部后仍继续下行，而导致吸样针205的针尖或样本容器91 (92)破损。
吸样针205固定在吸样针固定部件2015上，吸样针固定部件2015通过螺钉固定在沿Z1、Z2放置的直线滑轨2014上，同时螺母2013被卡在吸样针固定部件2015设置的卡槽中，并且螺母2013相和吸样针固定部件2015之间不产生相对转动。丝杠2012通过螺钉与步进电机2011转轴连接。步进电机2011可以带动螺杆2012转动，并驱动吸样针固定部件2015带着吸样针205沿Z1或Z2方向移动。吸样针205在Z1、Z2方向的初始位置通过位置传感器2017和设置在吸样针固定部件2015上的光耦传感器感应片(无图示)实现定位，并通过吸样针位置传感器实现吸样针205在沿Z1或Z2方向上移动定位，防止吸样针205的针尖到达样本容器91(92)的底部后仍继续下行。
在步进电机步进电机2001和步进电机2011驱动下，吸样针205可以沿Y1、Y2方向以及Z1、Z2方向做二维移动。可以实现通过吸吐动作对样本容器中的血液样本混匀并从混匀后的血液样本中吸取适量的血样、以及到制样器中分血样的功能。
在本实施方式中，通过吸样针205吸吐样本容器91(92)中的血液样本对其进行混匀的步骤如下：
控制装置21判断进样模式是第一进样模式还是第二进样模式；
如果是第一进样模式，则由运样装置17将样本架80上的样本容器91(92)运送至第一采样位；如果是第二进样模式，则由样本仓组件18将单个样本容器91(92)运送至第二采样位，其中第一采样位和第二采用位可以是同一位置，也可以是不同的位置；
由吸样针风干装置将吸样针205的外壁风干，并吸吐驱动装置驱动吸样针205吸取适量空气，使吸样针205内部形成一段隔离气柱；
吸样针移动组件201驱动吸样针205下行，由吸样针位置传感器或者吸样针驱动装置根据电机步数判断吸样针205的针尖是否到达样本容器91(92)的底部，如果吸样针205的针尖到达样本容器91(92)的底部，吸样针移动组件201停止驱动吸样针205下行，否则继续驱动吸样针205下行，直至到达样本容器91(92)的底部；
吸吐驱动装置驱动吸样针205吸入适量的血液样本后，将所吸入的血液样本回退至样本容器91(92)中，使得样本容器91(92)中的血液样本形成一定流动，直至血液样本混匀；
吸样针205从样本容器91(92)吸取适量的混匀后的血液样本，以进行血液样本采集。
在本实施方式中，样本容器91(92)所装的为全血样本，并由于直接用吸样针205吸吐全血样本并进行混匀，因此在混匀后吸样针205可直接吸取预定体积的全血样本即可，不需要清洗采样针。
本技术领域技术人员可以理解，本申请中已经讨论过的各种操作、方法、流程中的步骤、措施、方案可以被交替、更改、组合或删除。进一步地，具有本申请中已经讨论过的各种操作、方法、流程中的其他步骤、措施、方案也可以被交替、更改、重排、分解、组合或删除。进一步地，现有技术中的具有与本申请中公开的各种操作、方法、流程中的步骤、措施、方案也可以被交替、更改、重排、分解、组合或删除。
以上所述仅是本申请的部分实施方式，应当指出，对于本技术领域的普通技术人员来说，在不脱离本申请原理的前提下，还可以做出若干改进和润饰，这些改进和润饰也应视为本申请的保护范围。

Claims (50)

  1. 一种血样分析仪，包括：
    运样装置，用于运送装有第一和/或第二样本容器的样本架；
    第一混匀装置，具有用于搅拌第一样本容器中的血液样本的样本搅拌部件，所述第一混匀装置能够驱动所述样本搅拌部件对第一混匀位的所述样本架上的装有微量血液样本的所述第一样本容器中的血液样本进行混匀；
    第二混匀装置，能够获取所述样本架或所述样本架上的所述第二样本容器，并能够驱动第二混匀位上的装有常量血液样本的第二样本容器以进行血液样本的混匀；
    控制装置，与所述运样装置、所述第一混匀装置和所述第二混匀装置通信连接，控制所述运样装置、所述第一混匀装置和所述第二混匀装置的动作。
  2. 根据权利要求1所述的血样分析仪，其特征在于：所述运样装置能够将装有第一和/或第二样本容器的样本架运送至所述第一混匀位；所述第二混匀装置能够从所述第一混匀位获取所述样本架或所述样本架上的所述第二样本容器运送至所述第二混匀位。
  3. 根据权利要求1所述的血样分析仪，其特征在于：所述第一混匀位与所述第二混匀位为同一位置。
  4. 根据权利要求1所述的血样分析仪，其特征在于：所述样本搅拌部件的头部为圆柱型、桨状或多边形。
  5. 根据权利要求1所述的血样分析仪，其特征在于：所述控制装置控制所述样本搅拌部件以自转、圆周轨道、线性摆动或上下震荡方式中一种或几种方式的组合进行搅拌。
  6. 根据权利要求1所述的血样分析仪，其特征在于：所述样本搅拌部件能够进行上下移动，并能向下移动到所述第一混匀位上的第一样本容器中进行搅拌混匀。
  7. 根据权利要求1所述的血样分析仪，还包括：
    清洗部件，用于对所述样本搅拌部件进行清洗作业；优选地，所述清洗部件包括清洗液进口和清洗液排出口，以对位于所述清洗部件中的所述样本搅拌部件进行清洗作业；更优选地，所述清洗液排出口还能用于进行抽气，以此对所述样本搅拌部件进行干燥处理。
  8. 根据权利要求1所述的血样分析仪，其特征在于，还包括：
    清洗部件，所述清洗部件包括能够对所述样本搅拌部件进行清洗的清洗池。
  9. 根据权利要求1～8所述的血样分析仪，还包括：
    样本仓组件，包括样本仓盖和样本容器固定孔，用于放置在所述样本容器固定孔上的微量血样或常量血样的单样本进样。
  10. 根据权利要求9所述的血样分析仪，还包括：
    所述控制装置还用于判断当前进样模式是第一进样模式还是第二进样模式；
    当判断是所述第一进样模式时，控制所述运样装置运送装有所述第一和/或第二样本容器的所述样本架；
    当判断是所述第二进样模式时，控制所述样本仓组件运送单个所述第一和/或第二样本容器。
  11. 根据权利要求1所述的血样分析仪，其特征在于：所述运样装置将装有所述第一样本容器的样本架运送至预定位置，所述第二混匀装置的夹爪能够从所述预定位置的所述样本架上抓取所述第一样本容器到所述第一混匀位。
  12. 根据权利要求1～11任意一项所述的血样分析仪，还包括：
    测量模式设定装置，用于设置第一测量模式和第二测量模式；
    其中，所述控制装置根据所述测量模式设定装置的设定执行以下动作：
    (1)判断是所述第一测量模式还是所述第二测量模式；
    (2)当判断是所述第一测量模式时，控制所述第一混匀装置对所述第一样本容器中的血液样本进行混匀；
    (3)当判断是所述第二测量模式时，控制所述第二混匀装置抓取所述第二样本容器 进行混匀。
  13. 根据权利要求12所述的血样分析仪，还包括：
    吸样装置，用于从样本容器吸移经过混匀后的血液样本；
    当判断是所述第一测量模式时，所述控制装置控制所述吸样装置从所述第一样本容器中吸移第一采血量的血液样本；
    当判断是所述第二测量模式时，所述控制装置控制所述吸样装置从所述第二样本容器中吸移第二采血量的血液样本；
    其中，所述第一采样量少于所述第二采样量；优选的所述第一采样量为5-50μL，更优选的为15-35μL。
  14. 根据权利要求12所述的血样分析仪，其特征在于：
    所述测量模式设定装置还用于设置第三测量模式；
    当所述控制装置判断是所述第三测量模式时，控制所述第一混匀装置对所述第一样本容器中预稀释的血液样本进行混匀。
  15. 根据权利要求1～14任意一项所述的血样分析仪，其特征在于：所述第一混匀装置对所述第一样本容器的血液样本进行混匀，所述第一样本容器中的血液样本为全血样本；优选的，所述第一样本容器的血液样本为30-250μL，更优选的为50-200μL，更加优选地为50-100μL。
  16. 根据权利要求1～15所述的血样分析仪，其特征在于：所述第一混匀装置对所述第一样本容器的微量血液样本进行了混匀后，多次重复测量该微量血液样本的血红蛋白值的波动幅度不超过±2g/L。
  17. 一种血样分析仪，包括：
    样本仓组件，具有样本仓盖和样本容器固定孔，用于放置在所述样本容器固定孔上的微量血样或常量血样的单样本进样；
    混匀装置，具有用于搅拌所述样本容器中的血液样本的样本搅拌部件，所述混匀装置能够驱动所述样本搅拌部件对所述样本容器的血液样本进行混匀。
  18. 根据权利要求17所述的血样分析仪，其特征在于：所述血样分析仪仅用于处理微量全血样本和预稀释血样本。
  19. 根据权利要求17所述的血样分析仪，还包括：
    吸样装置，用于从所述样本容器吸移经过混匀后的血液样本；
    制样装置，用于将所述吸样装置吸移的所述血液样本制备成检测用试样；
    控制装置，与所述混匀装置、所述吸样装置和/或所述制样装置通信连接，控制所述混匀装置、所述吸样装置和/或所述制样装置的动作。
  20. 根据权利要求19所述的血样分析仪，还包括：
    测量模式设定装置，用于设置第一测量模式和第二测量模式；
    其中，所述控制装置根据所述测量模式设定装置的设定执行以下动作：
    (1)判断是所述第一测量模式还是所述第二测量模式；
    (2)判断是所述第一测量模式时，控制所述吸样装置从所述样本架上的所述样本容器中吸移第一采样量的所述血液样本，并控制所述制样装置制备第一检测用试样；
    (3)判断是所述第二测量模式时，控制所述吸样装置从所述样本架上的所述样本容器中吸移第二采样量的所述血液样本，并控制所述制样装置制备第二检测用试样；
    其中，所述第一采样量少于所述第二采样量；优选的所述第一采样量为5-50μL，更优选的为15-35μL。
  21. 根据权利要求20所述的血样分析仪，其特征在于：
    所述测量模式设定装置还用于设置第三测量模式；
    当所述控制装置判断是所述第三测量模式时，控制所述吸样装置从所述样本架上的所述样本容器中吸移第三采样量的预稀释血液样本，并控制所述制样装置制备第三检测用 试样。
  22. 一种血样分析仪，包括：
    第一混匀装置，能够对第一样本容器中的血液样本进行混匀；
    第二混匀装置，能够以不同于所述第一混匀装置的方式对所述第二样本容器中的血液样本进行混匀；
    控制装置，与所述第一混匀装置和所述第二混匀装置通信连接，并能够执行以下作业：
    (1)判断是第一测量模式还是第二测量模式；
    (2)判断是所述第一测量模式时，控制所述第一混匀装置对所述第一样本容器中的血液样本进行混匀；
    (3)判断是所述第二测量模式时，控制所述第二混匀装置对所述第二样本容器中的血液样本进行混匀。
  23. 根据权利要求22所述的血样分析仪，其特征在于，所述控制装置还能够执行以下作业：
    (1)判断是否为第三测量模式；
    (2)当所述控制装置判断是所述第三测量模式时，控制所述第一混匀装置对所述样本容器中预稀释的血液样本进行混匀。
  24. 根据权利要求22所述的血样分析仪，其特征在于，所述第一样本容器中的血液样本为全血样本，优选的第一样本容器中的血液样本体积为30-250μl，更优选50-200μl，更加优选50-100μl。
  25. 根据权利要求22～24任意一项所述的血样分析仪，还包括：
    样本仓组件，具有样本仓盖和样本容器固定孔，用于放置在所述样本容器固定孔上的血液样本的单样本进样；和/或
    运样装置，用于运送装有所述第一样本容器和/或所述第二样本容器的样本架。
  26. 根据权利要求25所述的血样分析仪，其特征在于，所述控制装置还能够执行以下作业：
    (1)判断是第一进样模式还是第二进样模式；
    (2)判断是所述第一进样模式时，控制所述运样装置运送装有所述样本容器的所述样本架；
    (3)判断是所述第二进样模式时，控制所述样本仓组件向所述血液分析仪运送单个所述样本容器。
  27. 一种血样分析仪，包括：
    运样装置，用于运送装有样本容器的样本架；
    混匀装置，具有用于搅拌所述样本容器中的血液样本的样本搅拌部件，所述混匀装置能够驱动所述样本搅拌部件对所述样本容器的血液样本进行混匀；
    控制装置，与所述运样装置和所述混匀装置通信连接，控制所述运样装置和所述混匀装置的动作。
  28. 一种用于血常规的血样分析方法，包括：
    将装有血液样本的样本容器运送至混匀位；
    驱动第一混匀装置的样本搅拌部件对所述样本容器中的血液样本进行混匀；
    从所述混匀位上的所述样本容器中吸移预定采样量的所述血液样本制备血常规检测项目用的检测用试样；
    检测所述检测用试样，获取所述血常规检测项目的相关指标。
  29. 根据权利要求28所述的血样分析方法，还包括：
    判断当前测量模式是第一测量模式还是第二测量模式；
    当判断是所述第一测量模式时，从第一样本容器中吸移第一采样量的混匀后血液样 本，并制备第一检测用试样；
    当判断是所述第二测量模式时，从第二样本容器中吸移第二采样量的混匀后血液样本，并制备第二检测用试样；
    其中，所述第一采样量少于所述第二采样量；优选的所述第一采样量为5-50μL，更优选的为15-35μL。
  30. 根据权利要求29所述的血样分析方法，还包括：
    判断当前测量模式是否为第三测量模式；
    当判断是所述第三测量模式时，从第一样本容器中吸移第三采样量的混匀后的预稀释血液样本，并制备第三检测用试样。
  31. 根据权利要求28～30任意一项所述的血样分析方法，还包括：
    判断当前进样模式是第一进样模式还是第二进样模式；
    当判断是所述第一进样模式时，由运样装置运送所述样本容器；
    当判断是所述第二进样模式时，由样本仓组件向血液分析仪运送单个所述样本容器。
  32. 根据权利要求28～30任意一项所述的血样分析方法，其特征在于：所述样本搅拌部件以自转、圆周轨道、线性摆动或上下震荡方式中一种或几种方式的组合进行搅拌。
  33. 根据权利要求28～30任意一项所述的血样分析方法，还包括：第二混匀装置获取第二样本容器进行颠倒混匀。
  34. 根据权利要求28～30任意一项所述的血样分析方法，其特征在于：所述第一样本容器中血液样本的量≤250μL；优选地，所述第一样本容器中血液样本的量为30～250μL，更优选地，所述第一样本容器中血液样本的量为50～200μL，更更优选地，所述第一样本容器中血液样本的量为50～100μL。
  35. 一种血样分析方法，包括：
    测量模式确定步骤：判断当前测量模式是第一测量模式还是第二测量模式；
    第一检测用试样制备步骤：当判断是所述第一测量模式时，控制第一混匀装置驱动样本搅拌部件对样本容器中的血液样本进行混匀，并吸移第一采样量的血液样本制备第一检测用试样；
    第二检测用试样制备步骤：当判断是所述第二测量模式时，控制第二混匀装置对所述样本容器中的血液样本进行混匀，并吸移第二采样量的血液样本制备第二检测用试样；及
    检测步骤：检测所述第一检测用试样或所述第二检测用试样。
  36. 根据权利要求35所述的血样分析方法，其特征在于：
    在所述测量模式确定步骤中，还判断所述当前测量模式是否为第三测量模式；
    第三检测用试样制备步骤：当判断是所述第三测量模式时，控制所述第一混匀装置对所述样本容器中的预稀释的血液样本进行混匀，并吸移第三采样量的所述预稀释血液样本制备第三检测用试样；
    在所述检测步骤中，检测所述第三检测用试样。
  37. 根据权利要求35或36所述的血样分析方法，还包括：
    进样模式确定步骤：判断当前进样模式是第一进样模式还是第二进样模式；
    样架运送步骤：当判断是所述第一进样模式时，控制运样装置将装有所述样本容器的所述样本架运送至预定位置，并将混匀后的样本容器运送至第一采样位；
    样本仓组件关闭步骤：当判断是所述第二进样模式时，关闭样本仓组件，所述样本容器被送至第二采样位。
  38. 根据权利要求35或36所述的血样分析方法，其特征在于：
    在第一检测用试样制备步骤中，第二混匀装置将由运样装置运送至预定位置的样本架上的样本容器搬运至第一混匀位置进行混匀；
    在第二检测用试样制备步骤中，由第二混匀装置抓取由运样装置运送至预定位置的 样本架或所述样本架上的所述样本容器进行颠倒混匀。
  39. 根据权利要求35～37任一项所述的血样分析方法，其特征在于：由所述第一混匀装置进行混匀的样本容器中的血液样本为全血样本，所述血液样本的量为30～250μL，更优选地，所述血液样本的量为50～200μL，更更优选地，血液样本的量为50～100μL。
  40. 一种血样分析仪，包括：
    运样装置，用于运送装有样本容器的样本架；
    混匀装置，具有用于吸吐样本容器中的血液样本的吸样装置，所述吸样装置能够驱动所述吸样装置的吸样针对采样位上装有微量全血血液样本的所述样本容器中的血液样本进行吸吐混匀；
    控制装置，与所述运样装置、所述混匀装置通信连接，控制所述运样装置、所述混匀装置的动作。
  41. 根据权利要求40所述的血样分析仪，其特征在于：所述吸样装置还包括吸吐驱动装置，用于驱动所述吸样针吸吐所述样本容器中的血液样本进行混匀，优选的所述吸吐驱动装置为注射器。
  42. 根据权利要求41所述的血样分析仪，其特征在于：所述吸吐驱动装置能够驱动所述吸样针在对所述样本容器中的血液样本进行混匀前吸取适量空气，使所述吸样针内部形成一段隔离气柱。
  43. 根据权利要求40所述的血样分析仪，其特征在于：还包括第二混匀装置，能够获取所述样本架上装有常量血液样本的样本容器，并进行颠倒混匀。
  44. 根据权利要求40所述的血样分析仪，其特征在于：所述吸样装置还包括吸样针风干装置，用于将所述吸样针的外壁风干。
  45. 根据权利要求40～44任意一项所述的血样分析仪，其特征在于：所述吸样装置还包吸样针位置传感器，用于感知所述吸样针的下行位置。
  46. 根据权利要求40～44任意一项所述的血样分析仪，其特征在于：所述微量全血血液样本的量为30～250μL，优选地，所述血液样本的量的为50～200μL，更优选地，血液样本的量为50～100μL。
  47. 一种血样混匀方法，包括：
    吸样针吸取适量空气，使所述吸样针内部形成一段隔离气柱；
    驱动所述吸样针下行，靠近样本容器的底部；
    驱动所述吸样针吸入适量的全血血液样本后，将所吸入的所述血液样本回退至所述样本容器中，使得所述样本容器中的所述血液样本形成一定流动，直至所述血液样本混匀。
  48. 根据权利要求47所述的血样混匀方法，还包括：
    判断进样模式是第一进样模式还是第二进样模式；
    如果是所述第一进样模式，则由运样装置将样本架上的所述样本容器运送至第一采样位；如果是所述第二进样模式，则由样本仓组件将单个所述样本容器运送至第二采样位。
  49. 一种用于血样分析仪的控制装置，包括：
    至少一个处理器；以及
    存储器，存储所述至少一个处理器可执行的指令，所述指令在被所述至少一个处理器执行时使得所述血样分析仪执行根据权利要求27至38、46至47中任一项所述的方法。
  50. 一种计算机存储介质，存储有计算机可执行指令，所述计算机可执行指令在血样分析仪的至少一个处理器执行时，使得所述血样分析仪执行根据权利要求28至39、47至48中任一项所述的方法。
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US20210239725A1 (en) * 2018-08-24 2021-08-05 Shenzhen Mindray Bio-Medical Electronics Co., Ltd. Blood sample analyzer and blood sample agitating method
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