WO2019177371A8 - 미생물을 이용한 폴리(3-하이드록시프로피오네이트-b-락테이트) 블록공중합체 - Google Patents

미생물을 이용한 폴리(3-하이드록시프로피오네이트-b-락테이트) 블록공중합체 Download PDF

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WO2019177371A8
WO2019177371A8 PCT/KR2019/002909 KR2019002909W WO2019177371A8 WO 2019177371 A8 WO2019177371 A8 WO 2019177371A8 KR 2019002909 W KR2019002909 W KR 2019002909W WO 2019177371 A8 WO2019177371 A8 WO 2019177371A8
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hydroxypropionate
lactate
block copolymer
biosynthesized
glycerol
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PCT/KR2019/002909
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WO2019177371A1 (ko
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강혜옥
강동균
김철웅
허인영
최정윤
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주식회사 엘지화학
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Priority to US16/754,480 priority Critical patent/US11286510B2/en
Priority to CN201980004611.4A priority patent/CN111194353B/zh
Priority to JP2020519969A priority patent/JP7102514B2/ja
Publication of WO2019177371A1 publication Critical patent/WO2019177371A1/ko
Publication of WO2019177371A8 publication Critical patent/WO2019177371A8/ko
Priority to US17/679,007 priority patent/US11845973B2/en

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Abstract

본 발명은, 신규한 3-하이드록시프로피오네이트-락테이트의 블록공중합체[P(3HP-b-LA)]{Method for preparing poly(3-hydroxypropionate-b- lactate) block copolymer by using microorganisms} 및 그 제조방법에 관한 것으로, 더욱 상세하게는 락트산을 생합성하지 못하도록 개량한 재조합 대장균을 이용하여, 배양 초기에 글리세롤을 탄소원으로 하여 3-하이드록시프로피오네이트를 생산하는 유전자들과 개량된 PHA 신타아제를 통해 P(3HP)를 생합성하는 제1 배양단계; 및 탄소원으로 글리세롤과 글루코오스가 함께 공급시 글루코오스만 선택적으로 대장균 내로 유입시키는 탄소 이화대사억제 시스템(Carbon catabolic repression system)을 이용하여 P(3HP) 생산을 억제시키고, IPTG 유도 시스템을 통해 락테이트 생성 효소와 락틸-CoA 전환 효소가 발현되도록 하여 중단된 P(3HP) 말단에 폴리락테이트를 생합성시키는 제2 배양단계;를 포함하는 3-하이드록시프로피오네이트-락테이트의 블록 공중합체를 제조하는 방법 및 이에 의해 제조된 3-하이드록시프로피오네이트-락테이트의 블록 공중합체를 제공한다.
PCT/KR2019/002909 2018-03-15 2019-03-13 미생물을 이용한 폴리(3-하이드록시프로피오네이트-b-락테이트) 블록공중합체 WO2019177371A1 (ko)

Priority Applications (4)

Application Number Priority Date Filing Date Title
US16/754,480 US11286510B2 (en) 2018-03-15 2019-03-13 Poly(3-hydroxypropionate-b-lactate) block copolymer using microorganisms
CN201980004611.4A CN111194353B (zh) 2018-03-15 2019-03-13 使用微生物的聚(3-羟基丙酸酯-b-乳酸酯)嵌段共聚物
JP2020519969A JP7102514B2 (ja) 2018-03-15 2019-03-13 微生物を用いたポリ（３－ヒドロキシプロピオネート－ｂ－ラクテート）ブロック共重合体
US17/679,007 US11845973B2 (en) 2018-03-15 2022-02-23 Poly(3-hydroxypropionate-b-lactate) block copolymer using microorganisms

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR10-2018-0030522 2018-03-15
KR1020180030522A KR102519456B1 (ko) 2018-03-15 2018-03-15 미생물을 이용한 폴리(3-하이드록시프로피오네이트-b-락테이트) 블록공중합체

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US16/754,480 A-371-Of-International US11286510B2 (en) 2018-03-15 2019-03-13 Poly(3-hydroxypropionate-b-lactate) block copolymer using microorganisms
US17/679,007 Division US11845973B2 (en) 2018-03-15 2022-02-23 Poly(3-hydroxypropionate-b-lactate) block copolymer using microorganisms

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WO2019177371A1 WO2019177371A1 (ko) 2019-09-19
WO2019177371A8 true WO2019177371A8 (ko) 2019-11-07

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US (2) US11286510B2 (ko)
JP (1) JP7102514B2 (ko)
KR (1) KR102519456B1 (ko)
CN (1) CN111194353B (ko)
WO (1) WO2019177371A1 (ko)

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JP5436966B2 (ja) 2009-08-03 2014-03-05 日立粉末冶金株式会社 焼結歯車の製造方法
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KR101505172B1 (ko) 2011-12-20 2015-03-24 삼성전자주식회사 3-히드록시프로피온산을 생산하는 재조합 미생물 및 이를 이용한 3-히드록시프로피온산의 생산방법
KR102088504B1 (ko) * 2015-09-03 2020-03-12 주식회사 엘지화학 3-하이드록시프로피오네이트, 2-하이드록시부티레이트 및 락테이트를 반복단위로 포함하는 삼중합체 및 이의 제조방법
KR102361618B1 (ko) 2018-01-08 2022-02-09 주식회사 엘지화학 2-하이드록시부티레이트 및 3-하이드록시부티레이트의 공중합체 및 이의 제조방법

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US20220251612A1 (en) 2022-08-11
US11845973B2 (en) 2023-12-19
KR102519456B1 (ko) 2023-04-06
CN111194353A (zh) 2020-05-22
WO2019177371A1 (ko) 2019-09-19
JP7102514B2 (ja) 2022-07-19
CN111194353B (zh) 2023-08-01
JP2020536546A (ja) 2020-12-17
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