WO2011130725A3 - Production of organic acids from xylose rich hydrolysate by bacterial fermentation - Google Patents

Production of organic acids from xylose rich hydrolysate by bacterial fermentation Download PDF

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Publication number
WO2011130725A3
WO2011130725A3 PCT/US2011/032803 US2011032803W WO2011130725A3 WO 2011130725 A3 WO2011130725 A3 WO 2011130725A3 US 2011032803 W US2011032803 W US 2011032803W WO 2011130725 A3 WO2011130725 A3 WO 2011130725A3
Authority
WO
WIPO (PCT)
Prior art keywords
ability
carbon
source
lignocellulosic hydrolysate
metabolic evolution
Prior art date
Application number
PCT/US2011/032803
Other languages
French (fr)
Other versions
WO2011130725A2 (en
Inventor
Tammy B. Grabar
William Houser
Original Assignee
Myriant Technologies Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Myriant Technologies Inc filed Critical Myriant Technologies Inc
Priority to BR112012026539A priority Critical patent/BR112012026539A2/en
Publication of WO2011130725A2 publication Critical patent/WO2011130725A2/en
Publication of WO2011130725A3 publication Critical patent/WO2011130725A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/24—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Enterobacteriaceae (F), e.g. Citrobacter, Serratia, Proteus, Providencia, Morganella, Yersinia
    • C07K14/245—Escherichia (G)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1205—Phosphotransferases with an alcohol group as acceptor (2.7.1), e.g. protein kinases
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/04—Alpha- or beta- amino acids
    • C12P13/14—Glutamic acid; Glutamine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/04—Alpha- or beta- amino acids
    • C12P13/20—Aspartic acid; Asparagine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
    • C12P7/42—Hydroxy-carboxylic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
    • C12P7/44—Polycarboxylic acids
    • C12P7/46—Dicarboxylic acids having four or less carbon atoms, e.g. fumaric acid, maleic acid
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
    • C12P7/56—Lactic acid
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
    • C12P7/58—Aldonic, ketoaldonic or saccharic acids

Abstract

This invention relates to the metabolic evolution of a microbial organism previously optimized for producing an organic acid in commercially significant quantities under fermentative conditions using a hexose sugar as sole source of carbon in a minimal mineral medium. As a result of this metabolic evolution, the microbial organism acquires the ability to use pentose sugars in non-detoxified lignocellulosic hydrolysate for its growth while retaining the original growth kinetics, the rate of organic acid production and the ability to use hexose sugars as a source of carbon. More specifically, this invention provides bacterial strains that have the ability to produce succinic acid using non-detoxified lignocellulosic hydrolysate as the source of organic carbon. This invention also discloses the genetic changes in the microorganism that arise during the course of metabolic evolution in the presence of non-detoxified lignocellulosic hydrolysate.
PCT/US2011/032803 2010-04-16 2011-04-16 Production of organic acids from xylose rich hydrolysate by bacterial fermentation WO2011130725A2 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
BR112012026539A BR112012026539A2 (en) 2010-04-16 2011-04-16 "production of organic acids from xylose-rich hydrolyzate by bacterial fermentation"

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US34269510P 2010-04-16 2010-04-16
US61/342,695 2010-04-16

Publications (2)

Publication Number Publication Date
WO2011130725A2 WO2011130725A2 (en) 2011-10-20
WO2011130725A3 true WO2011130725A3 (en) 2012-04-19

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US2011/032803 WO2011130725A2 (en) 2010-04-16 2011-04-16 Production of organic acids from xylose rich hydrolysate by bacterial fermentation

Country Status (2)

Country Link
BR (1) BR112012026539A2 (en)
WO (1) WO2011130725A2 (en)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104862321A (en) * 2015-06-01 2015-08-26 大连理工大学 Potassium ion transporting protein gene trkH as well as coded protein and application thereof
CN111218408B (en) * 2020-01-21 2021-05-04 天津科技大学 Aspergillus niger strain for efficiently producing malic acid, construction method and application

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2015034948A1 (en) 2013-09-03 2015-03-12 Myriant Corporation A process for manufacturing acrylic acid, acrylonitrile and 1,4-butanediol from 1,3-propanediol
CN104046586B (en) * 2014-07-02 2016-08-17 山东大学 One strain gene engineering bacterium and the application in producing (2R, 3R)-2,3-butanediol thereof
WO2016207147A1 (en) * 2015-06-22 2016-12-29 Dsm Ip Assets B.V. Process for enzymatic hydrolysis of lignocellulosic material and fermentation of sugars
CN109517776A (en) * 2018-11-16 2019-03-26 河北科技师范学院 A kind of Salmonella enteritidis icdA gene-deleted strain and its application

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6962794B2 (en) * 1995-05-05 2005-11-08 Genecor International, Inc. Application of glucose transport mutants for production of aromatic pathway compounds

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6962794B2 (en) * 1995-05-05 2005-11-08 Genecor International, Inc. Application of glucose transport mutants for production of aromatic pathway compounds

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
ALMEIDA, J. R. ET AL.: "Screening of Saccharomyces cerevisiae strains with respect to anaerobic growth in non-detoxified lignocellulose hydrolysate", BIORESOURCE TECHNOLOGY., vol. 100, no. 14, 28 March 2009 (2009-03-28), pages 3674 - 3677 *
GROSSIORD, B. P. ET AL.: "Characterization, expression, and mutation of the Lactococcus lactis gaIPMKTE genes, involved in galactose utilization via the Leloir pathway", JOURNAL OF BACTERIOLOGY., vol. 185, no. 3, February 2003 (2003-02-01), pages 870 - 878 *
TIAN, S. ET AL.: "Yeast strains for ethanol production from lignocellulosic hydrolysates during in situ detoxification", BIOTECHNOLOGY ADVANCES., vol. 27, no. 5, 22 April 2009 (2009-04-22), pages 656 - 660 *
ZHANG, X. ET AL.: "Metabolic evolution of energy-conserving pathways for succinate production in Escherichia coli", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES, vol. 106, no. 48, 16 November 2009 (2009-11-16), pages 20180 - 20185 *

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104862321A (en) * 2015-06-01 2015-08-26 大连理工大学 Potassium ion transporting protein gene trkH as well as coded protein and application thereof
CN104862321B (en) * 2015-06-01 2018-03-13 大连理工大学 Kalium ion transport GFP trkH, its encoding proteins and its application
CN111218408B (en) * 2020-01-21 2021-05-04 天津科技大学 Aspergillus niger strain for efficiently producing malic acid, construction method and application

Also Published As

Publication number Publication date
BR112012026539A2 (en) 2015-09-22
WO2011130725A2 (en) 2011-10-20

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