WO2019013493A3 - 고농도 세포 배양 방법을 이용한 다당체의 생산 방법 - Google Patents

고농도 세포 배양 방법을 이용한 다당체의 생산 방법 Download PDF

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Publication number
WO2019013493A3
WO2019013493A3 PCT/KR2018/007663 KR2018007663W WO2019013493A3 WO 2019013493 A3 WO2019013493 A3 WO 2019013493A3 KR 2018007663 W KR2018007663 W KR 2018007663W WO 2019013493 A3 WO2019013493 A3 WO 2019013493A3
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WO
WIPO (PCT)
Prior art keywords
concenration
cell culture
polysaccharide
producing polysaccharide
culturing
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PCT/KR2018/007663
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English (en)
French (fr)
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WO2019013493A2 (ko
Inventor
이창규
곽지윤
유민지
Original Assignee
(주)카보엑스퍼트
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Publication of WO2019013493A2 publication Critical patent/WO2019013493A2/ko
Publication of WO2019013493A3 publication Critical patent/WO2019013493A3/ko

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/04—Polysaccharides, i.e. compounds containing more than five saccharide radicals attached to each other by glycosidic bonds

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  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Microbiology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

본 발명은 1) 대장균(Escherchia coli) 변이체 TBP38 균주를 1차 탄소원으로 글루코오스를 포함하는 배지에서 배양하는 단계; 2) 상기 단계 1)의 배양액에 2차 탄소원을 첨가한 후 20~30시간 동안 배양하고 배양된 균체를 획득하는 단계; 및 3) 상기 단계 2)에서 획득한 배양된 균체로부터 다당체를 추출하는 단계를 포함하는 다당체의 생산 방법에 관한 것으로, 본 발명의 방법은 유용한 다당체의 생산성을 증대시키는데 이용될 수 있다.
PCT/KR2018/007663 2017-07-13 2018-07-05 고농도 세포 배양 방법을 이용한 다당체의 생산 방법 WO2019013493A2 (ko)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR10-2017-0089133 2017-07-13
KR1020170089133A KR101958017B1 (ko) 2017-07-13 2017-07-13 고농도 세포 배양 방법을 이용한 다당체의 생산 방법

Publications (2)

Publication Number Publication Date
WO2019013493A2 WO2019013493A2 (ko) 2019-01-17
WO2019013493A3 true WO2019013493A3 (ko) 2019-03-28

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PCT/KR2018/007663 WO2019013493A2 (ko) 2017-07-13 2018-07-05 고농도 세포 배양 방법을 이용한 다당체의 생산 방법

Country Status (2)

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KR (1) KR101958017B1 (ko)
WO (1) WO2019013493A2 (ko)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2021054493A1 (ko) * 2019-09-18 2021-03-25 (주)카보엑스퍼트 기능성 다당체의 제조방법 및 화장료 조성물
KR102251189B1 (ko) * 2019-09-18 2021-05-14 (주)카보엑스퍼트 기능성 다당체의 제조방법 및 화장료 조성물

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20010032497A (ko) * 1997-11-26 2001-04-25 에이.이. 스테일리 매니팩츄어링 컴파니 막 분리 단계를 포함하는 효소성 전분 당화
KR20160049158A (ko) * 2014-10-24 2016-05-09 충남대학교산학협력단 면역증강효과를 갖는 수용성 다당체 및 이를 함유하는 조성물

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20010032497A (ko) * 1997-11-26 2001-04-25 에이.이. 스테일리 매니팩츄어링 컴파니 막 분리 단계를 포함하는 효소성 전분 당화
KR20160049158A (ko) * 2014-10-24 2016-05-09 충남대학교산학협력단 면역증강효과를 갖는 수용성 다당체 및 이를 함유하는 조성물

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
CURATTI ET AL.: "Sucrose synthase is involved in the conversion of sucrose to polysaccharides in filamentous nitrogen-fixing cyanobacteria", PLANTA, vol. 228, no. 4, 2008, pages 617 - 625, XP019630361 *
PARK, JONG-TAE: "Role of maltose enzymes in glycogen synthesis by Escherichia coli", JOURNAL OF BACTERIOLOGY, vol. 193, no. 10, 2011, pages 2517 - 2526, XP055584163, ISSN: 0021-9193, DOI: 10.1128/JB.01238-10 *

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Publication number Publication date
KR20190007747A (ko) 2019-01-23
KR101958017B1 (ko) 2019-03-15
WO2019013493A2 (ko) 2019-01-17

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