WO2018038577A8 - 7-아미노세팔로스포란산의 고농도 생산 재조합 아크레모니움 크리소제눔 균주의 제조방법 및 이 방법으로 제조된 균주 - Google Patents

7-아미노세팔로스포란산의 고농도 생산 재조합 아크레모니움 크리소제눔 균주의 제조방법 및 이 방법으로 제조된 균주 Download PDF

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WO2018038577A8
WO2018038577A8 PCT/KR2017/009330 KR2017009330W WO2018038577A8 WO 2018038577 A8 WO2018038577 A8 WO 2018038577A8 KR 2017009330 W KR2017009330 W KR 2017009330W WO 2018038577 A8 WO2018038577 A8 WO 2018038577A8
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aca
acremonium chrysogenum
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WO2018038577A1 (ko
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신용철
표쩌
윤상활
이현서
최연희
김은정
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아미코젠주식회사
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Publication of WO2018038577A1 publication Critical patent/WO2018038577A1/ko
Publication of WO2018038577A8 publication Critical patent/WO2018038577A8/ko

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/78—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5)
    • C12N9/80—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5) acting on amide bonds in linear amides (3.5.1)
    • C—CHEMISTRY; METALLURGY
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    • C12P35/00—Preparation of compounds having a 5-thia-1-azabicyclo [4.2.0] octane ring system, e.g. cephalosporin
    • C12P35/06—Cephalosporin C; Derivatives thereof
    • C—CHEMISTRY; METALLURGY
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    • C12Y—ENZYMES
    • C12Y305/00—Hydrolases acting on carbon-nitrogen bonds, other than peptide bonds (3.5)
    • C12Y305/01—Hydrolases acting on carbon-nitrogen bonds, other than peptide bonds (3.5) in linear amides (3.5.1)
    • C12Y305/01093—Glutaryl-7-aminocephalosporanic-acid acylase (3.5.1.93)

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Abstract

본 발명은 7-아미노세팔로스포란산의 고농도 생산 재조합 아크레모니움 크리 소제눔 균주의 제조방법 및 이 방법으로 제조된 균주에 관한 것이며, 보다 상세하 게는 7-ACA 고생산성 아크레모니움 크리소제눔 균주의 제조 방법, 이 방법으로 제 조된 재조합 아크레모니움 크리소제눔 균주, 상기 균주를 7-ACA를 생산할 수 있는 조건에서 배양하고, 이를 회수하는 7-ACA의 생산방법, 상기 제조방법에 이용된 아 크레모니움 크리소제눔 균주에서의 단백질 고발현용 프로모터, 아크레모니움 크리 소제눔 균주에서의 CPC 아실라제 고발현용 발현 백터, 상기 균주를 7-ACA 생산에 사용하는 용도, 상기 생산된 7— ACA를 항생제 제조에 사용하는 용도 및 상기 7-ACA 를 투여하는 것을 포함하는 세균 감염성 질환의 치료방법에 관한 것이다. 본 발명 의 방법에 의하면 재조합 아크레모니움 크리소제눔 균주를 제조하여 이의 발효를 통하여 7-ACA를 고농도로 제조할수 있다.
PCT/KR2017/009330 2016-08-26 2017-08-25 7-아미노세팔로스포란산의 고농도 생산 재조합 아크레모니움 크리소제눔 균주의 제조방법 및 이 방법으로 제조된 균주 WO2018038577A1 (ko)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201780050799.7A CN110214188B (zh) 2016-08-26 2017-08-25 7-氨基头孢烷酸的高浓度生产重组顶头孢霉菌株的制造方法及利用其方法所制造的菌株

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KR1020160109365A KR101808192B1 (ko) 2016-08-26 2016-08-26 7-아미노세팔로스포란산의 고농도 생산 재조합 아크레모니움 크리소제눔 균주의 제조방법 및 이 방법으로 제조된 균주
KR10-2016-0109365 2016-08-26

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WO2018038577A1 WO2018038577A1 (ko) 2018-03-01
WO2018038577A8 true WO2018038577A8 (ko) 2019-02-21

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WO (1) WO2018038577A1 (ko)

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CN110214188A (zh) 2019-09-06
WO2018038577A1 (ko) 2018-03-01
CN110214188B (zh) 2023-06-06

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