ES2156812B1 - Una proteasa extracelular de acremonium chrysogenum con actividad cpc-acetilhidrolasa y su utilizacion para la sintesis de derivados desacetilados de cefalosporina c e inactivacion del gen para aumentar la produccion de cefalosporina. - Google Patents

Una proteasa extracelular de acremonium chrysogenum con actividad cpc-acetilhidrolasa y su utilizacion para la sintesis de derivados desacetilados de cefalosporina c e inactivacion del gen para aumentar la produccion de cefalosporina.

Info

Publication number
ES2156812B1
ES2156812B1 ES009900731A ES9900731A ES2156812B1 ES 2156812 B1 ES2156812 B1 ES 2156812B1 ES 009900731 A ES009900731 A ES 009900731A ES 9900731 A ES9900731 A ES 9900731A ES 2156812 B1 ES2156812 B1 ES 2156812B1
Authority
ES
Spain
Prior art keywords
cephalosporin
cpc
gene
production
activity
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
ES009900731A
Other languages
English (en)
Other versions
ES2156812A1 (es
Inventor
Alvarez Javier Velasco
Martin Santiago Gutierrez
Blanco Francisco J Casqueiro
Garcia Sonia Campoy
Fierro Francisco Fierro
Fuente Jose Luis Barredo
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Antibioticos SA
Original Assignee
Antibioticos SA
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Antibioticos SA filed Critical Antibioticos SA
Priority to ES009900731A priority Critical patent/ES2156812B1/es
Priority to PCT/ES2000/000126 priority patent/WO2000061767A1/es
Priority to JP2000611690A priority patent/JP2002541812A/ja
Priority to EP00914189A priority patent/EP1170369A1/en
Priority to US09/958,413 priority patent/US6815189B1/en
Priority to AU35595/00A priority patent/AU3559500A/en
Publication of ES2156812A1 publication Critical patent/ES2156812A1/es
Application granted granted Critical
Publication of ES2156812B1 publication Critical patent/ES2156812B1/es
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/18—Carboxylic ester hydrolases (3.1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/58—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from fungi
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P35/00—Preparation of compounds having a 5-thia-1-azabicyclo [4.2.0] octane ring system, e.g. cephalosporin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P35/00—Preparation of compounds having a 5-thia-1-azabicyclo [4.2.0] octane ring system, e.g. cephalosporin
    • C12P35/02—Preparation of compounds having a 5-thia-1-azabicyclo [4.2.0] octane ring system, e.g. cephalosporin by desacylation of the substituent in the 7 position
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P35/00—Preparation of compounds having a 5-thia-1-azabicyclo [4.2.0] octane ring system, e.g. cephalosporin
    • C12P35/06—Cephalosporin C; Derivatives thereof

Abstract

Una proteasa extracelular de Acremonium chrysogenum con actividad CPC-acetilhidrolasa y su utilización para la síntesis de derivados desacetilados de cefalosporina C e inactivación del gen para aumentar la producción de cefaloporina. El procedimiento incluye la preparación de una enzima con actividad CPC- acetilhidrolasa (CPC-AH) a partir de caldos de cultivo del hongo filamentoso A.chrysogenum y la caracterización bioquímica de dicha enzima. Asimismo, describe la clonación y secuenciación de un fragmento de ADN genómico y otro de ADN complementario (ADNc) que contienen el gen cahB que codifica para dicha enzima. Mediante el uso del gen en forma de ADNc se describe la producción de la actividad enzimática CPC-AH B en Escherichia coli. Finalmente, se incluye un procedimiento para aumentar la producción de cefalosporina en A. chrysogenum mediante la inactivación del gen cahB.
ES009900731A 1999-04-09 1999-04-09 Una proteasa extracelular de acremonium chrysogenum con actividad cpc-acetilhidrolasa y su utilizacion para la sintesis de derivados desacetilados de cefalosporina c e inactivacion del gen para aumentar la produccion de cefalosporina. Expired - Fee Related ES2156812B1 (es)

Priority Applications (6)

Application Number Priority Date Filing Date Title
ES009900731A ES2156812B1 (es) 1999-04-09 1999-04-09 Una proteasa extracelular de acremonium chrysogenum con actividad cpc-acetilhidrolasa y su utilizacion para la sintesis de derivados desacetilados de cefalosporina c e inactivacion del gen para aumentar la produccion de cefalosporina.
PCT/ES2000/000126 WO2000061767A1 (es) 1999-04-09 2000-04-07 Una proteasa extracelular de $i(acremonium chrysogenum) con actividad cpc-acetilhidrolasa y su utilizacion para la sintesis de derivados desacetilados de cefalosporina c e inactivacion del gen para aumentar la produccion de cefalosporina
JP2000611690A JP2002541812A (ja) 1999-04-09 2000-04-07 ＣＰＣアセチルヒドロラーゼ活性を有するＡｃｒｅｍｏｎｉｕｍｃｈｒｙｓｏｇｅｎｕｍの細胞外プロテアーゼならびにセファロスポリンＣの脱アセチル化誘導体の合成およびセファロスポリンの収率を上昇させるための遺伝子の不活性化におけるその使用
EP00914189A EP1170369A1 (en) 1999-04-09 2000-04-07 Extracellular protease from acremonium chrysogenum with cpc-acetyl hydrolase activity and its utilization in the synthesis of deacetylated derivatives of cephalosporin c and inactivation of the gene for increasing production of cephalosporin
US09/958,413 US6815189B1 (en) 1999-04-09 2000-04-07 Gene from acremonium chrysogenum encoding a protein with cephalosporin C acetylhydrolase activity and methods of use of such gene
AU35595/00A AU3559500A (en) 1999-04-09 2000-04-07 Extracellular protease from (acremonium chrysogenum) with cpc-acetyl hydrolase activity and its utilization in the synthesis of deacetylated derivatives of cephalosporin c and inactivation of the gene for increasing production of cephalosporin

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
ES009900731A ES2156812B1 (es) 1999-04-09 1999-04-09 Una proteasa extracelular de acremonium chrysogenum con actividad cpc-acetilhidrolasa y su utilizacion para la sintesis de derivados desacetilados de cefalosporina c e inactivacion del gen para aumentar la produccion de cefalosporina.

Publications (2)

Publication Number Publication Date
ES2156812A1 ES2156812A1 (es) 2001-07-16
ES2156812B1 true ES2156812B1 (es) 2002-02-01

Family

ID=8307966

Family Applications (1)

Application Number Title Priority Date Filing Date
ES009900731A Expired - Fee Related ES2156812B1 (es) 1999-04-09 1999-04-09 Una proteasa extracelular de acremonium chrysogenum con actividad cpc-acetilhidrolasa y su utilizacion para la sintesis de derivados desacetilados de cefalosporina c e inactivacion del gen para aumentar la produccion de cefalosporina.

Country Status (6)

Country Link
US (1) US6815189B1 (es)
EP (1) EP1170369A1 (es)
JP (1) JP2002541812A (es)
AU (1) AU3559500A (es)
ES (1) ES2156812B1 (es)
WO (1) WO2000061767A1 (es)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7376587B1 (en) 2000-07-11 2008-05-20 Western Union Financial Services, Inc. Method for enabling transfer of funds through a computer network
US8897536B2 (en) * 2011-04-24 2014-11-25 Universidade Da Coruna. Otri Molecular block-matching method for gel image analysis
CN102757999A (zh) * 2011-04-29 2012-10-31 上海医药工业研究院 一种用于生产7-氨基头孢烷酸的发酵培养基及其发酵方法
MD4186C1 (ro) * 2012-02-20 2013-06-30 Институт Микробиологии И Биотехнологии Академии Наук Молдовы Tulpină de fungi Fusarium gibbosum - producătoare de proteaze acide şi neutre, xilanaze şi b-glucozidaze
MD4285C1 (ro) * 2013-02-28 2014-12-31 Институт Микробиологии И Биотехнологии Академии Наук Молдовы Tulpină de fungi Trichoderma koningii Oudemans - producătoare de proteaze acide, neutre şi alcaline
KR101808192B1 (ko) * 2016-08-26 2018-01-18 아미코젠주식회사 7-아미노세팔로스포란산의 고농도 생산 재조합 아크레모니움 크리소제눔 균주의 제조방법 및 이 방법으로 제조된 균주

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2040707C (en) * 1990-04-27 2002-07-09 Kenji Mitsushima Cephalosporin acetylhydrolase gene and protein encoded by said gene
KR100270508B1 (ko) 1998-04-24 2001-02-01 고두모 신규세파로스포린디아세틸라아제유전자및이를이용한단백질제조방법

Also Published As

Publication number Publication date
EP1170369A1 (en) 2002-01-09
JP2002541812A (ja) 2002-12-10
ES2156812A1 (es) 2001-07-16
AU3559500A (en) 2000-11-14
WO2000061767A1 (es) 2000-10-19
US6815189B1 (en) 2004-11-09

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