WO2017134306A1 - Cd8 binding agents - Google Patents

Cd8 binding agents Download PDF

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Publication number
WO2017134306A1
WO2017134306A1 PCT/EP2017/052553 EP2017052553W WO2017134306A1 WO 2017134306 A1 WO2017134306 A1 WO 2017134306A1 EP 2017052553 W EP2017052553 W EP 2017052553W WO 2017134306 A1 WO2017134306 A1 WO 2017134306A1
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Prior art keywords
seq
cancer
binding agent
amino acid
acid sequence
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PCT/EP2017/052553
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English (en)
French (fr)
Inventor
Jan Tavernier
Anje CAUWELS
Nikolai Kley
Sarah Gerlo
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Universiteit Gent
Vlaams Instituut voor Biotechnologie VIB
Orionis Biosciences NV
Original Assignee
Universiteit Gent
Vlaams Instituut voor Biotechnologie VIB
Orionis Biosciences NV
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Application filed by Universiteit Gent, Vlaams Instituut voor Biotechnologie VIB, Orionis Biosciences NV filed Critical Universiteit Gent
Priority to US16/075,317 priority Critical patent/US11236166B2/en
Priority to CN201780021559.4A priority patent/CN109071627B/zh
Priority to EP17704207.4A priority patent/EP3411397A1/en
Priority to EP21195070.4A priority patent/EP3998281A1/en
Priority to JP2018540141A priority patent/JP6991979B2/ja
Priority to CA3013558A priority patent/CA3013558C/en
Publication of WO2017134306A1 publication Critical patent/WO2017134306A1/en
Anticipated expiration legal-status Critical
Priority to US17/554,180 priority patent/US12364753B2/en
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    • C07K16/2803—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against the immunoglobulin superfamily
    • C07K16/2815—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against the immunoglobulin superfamily against CD8
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Definitions

  • the present invention relates, in part, to binding agents (e.g., antibodies, such as, without limitation, VHHs) which bind CD8 and their use as therapeutic and diagnostic agents.
  • binding agents e.g., antibodies, such as, without limitation, VHHs
  • cancer Despite major advances in medicine, cancer remains one of the leading causes of death globally, with an estimated 12.7 million cases each year.
  • One of the major stumbling blocks in designing effective anticancer therapy is cancer immune evasion, in which cancer cells evade immune surveillance and destruction thereby resulting in clinically overt cancer.
  • Mechanisms of immune evasion include the selection of tumor variants resistant to immune effectors and the progressive formation of an immune suppressive environment within the tumor.
  • CD8 + T lymphocytes also known as cytotoxic T cells or CTLs
  • CD8+ CTLs curb cancer development by mechanisms including production of interferon (IFN)-Y and cytotoxins, exocytosis of lytic proteins (e.g., perforin, granzymes), and receptor-ligand binding of FAS molecules.
  • IFN interferon
  • lytic proteins e.g., perforin, granzymes
  • receptor-ligand binding of FAS molecules e.g., FAS molecules.
  • tumors can evade immune surveillance by crippling CTL functionality via, for instance, production of immune suppressive cytokines and engagement of immune checkpoint inhibition, either by the cancer cells themselves or by non-cancerous cells present in the tumor microenvironment. Further still, cancer cells have been shown to delete CTLs through apoptosis.
  • immunotherapeutic agents including, for example, those that can effectively derail tumor evasion and enhance anti-tumor immunity as mediated, for example, by CTLs.
  • the present invention relates to CD8 binding agents having at least one targeting moiety that specifically binds to CD8.
  • these CD8 binding agents bind to, but do not functionally modulate (including, without limitation, partially or fully neutralizing) CD8. Therefore, in various embodiments, the present CD8 binding agents have use in, for instance, recruiting a CD8-expressing cell to a site of interest while still allowing the CD8-expressing cell to signal via CD8 (i.e. the binding of the CD8 binding agent does not reduce or eliminate CD8 signaling at the site of interest).
  • the targeting moiety is a single domain antibody (NANOBODY or VHH).
  • the CD8 binding agent further comprises a signaling agent, e.g., without limitation, an interferon, an interleukin, and a tumor necrosis factor, that may be modified to attenuate activity.
  • the CD8 binding agent comprises additional targeting moieties that bind to other antigens of interest.
  • the other antigens of interest are present on tumor cells.
  • the other antigens of interest are present on immune cells.
  • the present CD8 binding agent may directly or indirectly recruit an immune cell, e.g. an immune cell that can kill and/or suppress a tumor cell (e.g., cytotoxic T cells), to a site of action (such as, by way of non-limiting example, the tumor microenvironment).
  • the present CD8 binding agents find use in the treatment of various diseases or disorders such as cancer, infections, immune disorders, autoimmune diseases, and other diseases and disorders, and the present invention encompasses various methods of treatment.
  • Figure 1 panel A, provides histograms showing the binding of six VHHs specific for mouse CD8 to CHO cells transfected with CD8a.
  • Panel B provides histograms showing mouse splenocytes stained with six VHHs specific for mouse CD8.
  • the relative binding affinities of the six VHHs are as follows: 1-11269 > 1-11265 and 1-11268> I- 11278 and 1-11287.
  • Figure 2 provides histograms depicting the effects of various VHHs specific for mouse CD8 on OVA-induced T cell proliferation and activation.
  • Figure 3 shows that the administration of a fusion of a VHH specific for mouse CD8 with a modified human interferon (Q124R mutant) led to reductions in tumor size.
  • mCD8Nb(R1) corresponds to clone R1 CDE28
  • mCD8Nb(R2) corresponds to clone R2CDE71.
  • Figure 4 shows that the administration of a fusion of a VHH specific for mouse CD8 with a modified human interferon (Q124R mutant) did not result in weight loss or hematological toxicity.
  • the histograms from left to right are: naive mice, PBS, untargeted chimera ⁇ i.e. a modified IFN, Q124R, targeting moiety for an irrelevant target), and two CD8 VHHs fused to a modified human IFN (Q124R).
  • mCD8Nb(R1) corresponds to clone R1 CDE28
  • mCD8Nb(R2) corresponds to clone R2CDE71.
  • Figure 5 depicts the binding characteristics of anti-human CD8 VHHs on HekT cells.
  • FIG. 6 panels A-C, depict the binding characteristics of anti-human CD8 VHHs.
  • Panel A shows cellular florescence as detected by flow cytometry.
  • Panel B shows median fluorescence intensity (MFI), which was calculated for five VHH dilutions and compared to binding obtained with a control VHH.
  • Panel C shows the percentage of human peripheral blood mononuclear cells (PMBCs) that bind the CD8 VHH (His+) among CD3- antigen presenting cells. Calculation was performed for five VHH dilutions and compared to binding obtained with the control VHH.
  • PMBCs human peripheral blood mononuclear cells
  • Figure 7 shows a mouse tumor growth study in which C57BL/6 mice were inoculated subcutaneously with B16 melanoma tumor cells. Perilesional treatment with the indicated treatment agents was started when tumors reached certain size as measured by caliper. Graph shows the evolution of tumor size over the indicated time.
  • Figure 8 shows a mouse tumor growth study in which mice were inoculated with 4T1 mammary tumor cells. The mice were treated with the indicated agents. Graphs show the evolution of tumor size over the indicated time.
  • Figure 9 shows a mouse tumor growth study in which mice were inoculated with 4T1 mammary tumor cells. The mice were then treated with the indicated agents with or without doxorubicin. Graphs show the evolution of tumor size over the indicated time.
  • Figure 10 shows, in panels A, B, and C, human CD8 targeting of mono-specific chimeras.
  • Zebra-plot of CD8 versus pSTATI staining of stimulated PBMCs is shown in panel A.
  • Panels B and C mean fluorescent intensities (MFI) of pSTATI staining of CD8-positive (panel B) or CD8-negative (panel C) are plotted (in both panels B and C, the order of the curves at X-axis point 100 is: anti-human CD8 VHH/human IFN R149A, anti-BclH O VHH/human IFN R149A, and anti-human CD8 VHH/human IFN R33A/E120A).
  • MFI mean fluorescent intensities
  • the present invention is based, in part, on the discovery of agents ⁇ e.g. antibodies such as, by way of non- limiting example, VHHs) that recognize and bind to CD8.
  • these CD8 binding agents bind to, but do not functionally modulate CD8.
  • these CD8 binding agents may bind and directly or indirectly recruit immune cells to sites in need of therapeutic action ⁇ e.g. a tumor).
  • the present invention provides pharmaceutical compositions comprising the CD8 binding agents and their use in the treatment of various diseases.
  • the present CD8 binding agent is a protein-based agent capable of specific binding to CD8.
  • the present CD8 binding agent is a protein-based agent capable of specific binding to CD8 without functionally modulation ⁇ e.g. partial or complete neutralization) of CD8.
  • CD8 is a heterodimeric type I transmembrane glycoprotein, whose a and ⁇ chains are both composed of an immunoglobulin (Ig)-like extracellular domain connected by an extended O-glycosylated stalk to a single-pass transmembrane domain and a short cytoplasmic tail (Li ef a/., 2013).
  • the cytoplasmic region of the a-chain contains two cysteine motifs that serve as a docking site for src tyrosine kinase p56lck (Lck). In contrast, this Lck binding domain appears to be absent from the ⁇ chain, suggesting that the CD8 ⁇ chain is not involved in downstream signaling (Artyomov ef a/., 2010).
  • CD8 functions as a co-receptor for the T-cell receptor with its principle role being the recruitment of Lck to the TCR-pMHC complex following co-receptor binding to MHC (Turner ef a/., 1990, Veillette ef a/., 1988).
  • the CD8 binding agent of the invention comprises a targeting moiety having an antigen recognition domain that recognizes an epitope present on the CD8 a and/or ⁇ chains.
  • the antigen-recognition domain recognizes one or more linear epitopes on the CD8 a and/or ⁇ chains.
  • a linear epitope refers to any continuous sequence of amino acids present on the CD8 a and/or ⁇ chains.
  • the antigen-recognition domain recognizes one or more conformational epitopes present on the CD8 a and/or ⁇ chains.
  • a conformation epitope refers to one or more sections of amino acids (which may be discontinuous) which form a three-dimensional surface with features and/or shapes and/or tertiary structures capable of being recognized by an antigen recognition domain.
  • the CD8 binding agent of the present invention may bind to the full-length and/or mature forms and/or isoforms and/or splice variants and/or fragments and/or any other naturally occurring or synthetic analogs, variants, or mutants of human CD8 a and/or ⁇ chains.
  • the CD8 binding agent of the invention may bind to any forms of the human CD8 a and/or ⁇ chains, including monomeric, dimeric, heterodimeric, multimeric and associated forms.
  • the CD8 binding agent binds to the monomeric form of CD8 a chain or CD8 ⁇ chain.
  • the CD8 binding agent binds to a homodimeric form comprised of two CD8 a chains or two CD8 ⁇ chains. In a further embodiment, the CD8 binding agent binds to a heterodimeric form comprised of one CD8 a chain and one CD8 ⁇ chain.
  • the present CD8 binding agent comprises a targeting moiety with an antigen recognition domain that recognizes one or more epitopes present on the human CD8 a chain.
  • the human CD8 a chain comprises the amino acid sequence of:
  • the human CD8 a chain comprises the amino acid sequence of:
  • the human CD8 a chain comprises the amino acid sequence of:
  • the present CD8 binding agent comprises a targeting moiety with an antigen recognition domain that recognizes one or more epitopes present on the human CD8 ⁇ chain.
  • the human CD8 ⁇ chain comprises the amino acid sequence of:
  • the human CD8 ⁇ chain comprises the amino acid sequence of:
  • the human CD8 ⁇ chain comprises the amino acid sequence of:
  • the human CD8 ⁇ chain comprises the amino acid sequence of:
  • the human CD8 ⁇ chain comprises the amino acid sequence of:
  • the human CD8 ⁇ chain comprises the amino acid sequence of:
  • the human CD8 ⁇ chain comprises the amino acid sequence of:
  • the human CD8 ⁇ chain comprises the amino acid sequence of:
  • the present CD8 binding agent comprises a targeting moiety capable of specific binding.
  • the CD8 binding agent comprises a targeting moiety having an antigen recognition domain such as an antibody or derivatives thereof.
  • the CD8 binding agent comprises a targeting moiety which is an antibody.
  • the antibody is a full-length multimeric protein that includes two heavy chains and two light chains. Each heavy chain includes one variable region (e.g., VH) and at least three constant regions (e.g., CHi, CH2 and CH3), and each light chain includes one variable region (VL) and one constant region (CL). The variable regions determine the specificity of the antibody.
  • Each variable region comprises three hypervariable regions also known as complementarity determining regions (CDRs) flanked by four relatively conserved framework regions (FRs).
  • CDRs complementarity determining regions
  • FRs relatively conserved framework regions
  • the three CDRs referred to as CDR1 , CDR2, and CDR3, contribute to the antibody binding specificity.
  • the antibody is a chimeric antibody. In some embodiments, the antibody is a humanized antibody.
  • the CD8 binding agent comprises a targeting moiety which is an antibody derivative or format.
  • the present CD8 binding agent comprises a targeting moiety which is a single- domain antibody, a recombinant heavy-chain-only antibody (VHH), a single-chain antibody (scFv), a shark heavy-chain-only antibody (VNAR), a microprotein (cysteine knot protein, knottin), a DARPin; a Tetranectin; an Affibody; a Transbody; an Anticalin; an AdNectin; an Affilin; an Affimer, a Microbody; a peptide aptamer; an alterase; a plastic antibody; a phylomer; a stradobody; a maxibody; an evibody; a fynomer, an armadillo repeat protein, a Kunitz domain, an avimer, an atrimer, a probody, an immunobody,
  • the CD8 binding agent comprises a targeting moiety which is a single-domain antibody, such as a VHH.
  • the VHH may be derived from, for example, an organism that produces VHH antibody such as a camelid, a shark, or the VHH may be a designed VHH.
  • VHHs are antibody-derived therapeutic proteins that contain the unique structural and functional properties of naturally-occurring heavy-chain antibodies. VHH technology is based on fully functional antibodies from camelids that lack light chains. These heavy-chain antibodies contain a single variable domain (VHH) and two constant domains (CH2 and CH3). VHHs are commercially available under the trademark of NANOBODY or NANOBODIES.
  • the CD8 binding agent comprises a VHH.
  • the CD8 binding agent comprises a targeting moiety which is a VHH comprising a single amino acid chain having four "framework regions” or FRs and three “complementary determining regions” or CDRs.
  • framework region or “FR” refers to a region in the variable domain which is located between the CDRs.
  • complementary determining region or “CDR” refers to variable regions in VHHs that contains the amino acid sequences capable of specifically binding to antigenic targets.
  • the CD8 binding agent comprises a VHH having a variable domain comprising at least one CDR1 , CDR2, and/or CDR3 sequences.
  • the CDR1 sequence is selected from:
  • the CDR2 sequence is selected from:
  • the CDR3 sequence is selected from:
  • AGSLYTCVQSIVWPARPYYDMDY (SEQ ID NO:18).
  • the CD8 binding agent comprises SEQ ID NO:12, SEQ ID NO:14, and SEQ ID NO:16. In various embodiments, the CD8 binding agent comprises SEQ ID NO:12, SEQ ID NO:14, and SEQ ID NO:17. In various embodiments, the CD8 binding agent comprises SEQ ID NO:12, SEQ ID NO:14, and SEQ ID NO:18.
  • the CD8 binding agent comprises SEQ ID NO:12, SEQ ID NO:15, and SEQ ID NO:16. In various embodiments, the CD8 binding agent comprises SEQ ID NO:12, SEQ ID NO:15, and SEQ ID NO:17. In various embodiments, the CD8 binding agent comprises SEQ ID NO:12, SEQ ID NO:15, and SEQ ID NO:18.
  • the CD8 binding agent comprises SEQ ID NO:13, SEQ ID NO:14, and SEQ ID NO:16. In various embodiments, the CD8 binding agent comprises SEQ ID NO:13, SEQ ID NO:14, and SEQ ID NO:17. In various embodiments, the CD8 binding agent comprises SEQ ID NO:13, SEQ ID NO:14, and SEQ ID NO:18. In various embodiments, the CD8 binding agent comprises SEQ ID N0:13, SEQ ID N0:15, and SEQ ID N0:16. In various embodiments, the CD8 binding agent comprises SEQ ID N0:13, SEQ ID N0:15, and SEQ ID N0:17. In various embodiments, the CD8 binding agent comprises SEQ ID N0:13, SEQ ID N0:15, and SEQ ID N0:18.
  • the CD8 binding agent comprises an amino acid sequence selected from the following sequences:
  • R3HCD129 (SEQ ID NO:20)
  • the CD8 binding agent comprises an amino acid sequence described in US Patent Publication No. 2014/0271462, the entire contents of which are incorporated by reference. In various embodiments, the CD8 binding agent comprises an amino acid sequence described in Table 0.1 , Table 0.2, Table 0.3, and/or Figures 1A-12I of US Patent Publication No. 2014/0271462, the entire contents of which are incorporated by reference.
  • the CD8 binding agent comprises a HCDR1 of a HCDR1 of SEQ ID NO: 22 or 23 and/or a HCDR2 of HCDR1 of SEQ ID NO: 22 or 23 and/or a HCDR3 of HCDR1 of SEQ ID NO: 22 or 23 and/or a LCDR1 of LCDR1 of SEQ ID NO: 24 and/or a LCDR2 of LCDR1 of SEQ ID NO: 24 and/or a LCDR3 of LCDR1 of SEQ ID NO: 24.
  • the present invention contemplates the use of any natural or synthetic analogs, mutants, variants, alleles, homologs and orthologs (herein collectively referred to as "analogs") of the CD8 binding agent of the invention as described herein.
  • the amino acid sequence of the CD8 binding agent further includes an amino acid analog, an amino acid derivative, or other non-classical amino acids.
  • the CD8 binding agent comprises a targeting moiety comprising a sequence that is at least 60% identical to any one of SEQ ID NOs: 12-24.
  • the CD8 binding agent may comprise a targeting moiety comprising a sequence that is at least about 60%, at least about 61%, at least about 62%, at least about 63%, at least about 64%, at least about 65%, at least about 66%, at least about 67%, at least about 68%, at least about 69%, at least about 70%, at least about 71%, at least about 72%, at least about 73%, at least about 74%, at least about 75%, at least about 76%, at least about 77%, at least about 78%, at least about 79%, at least about 80%, at least about 81%, at least about 82%, at least about 83%, at least about 84%, at least about 85%, at least about 86%, at least about 87%, at least about 88%, at least about 89%, at least about 90%, at least about 91%, at least about 9
  • the CD8 binding agent comprises a targeting moiety comprising an amino acid sequence having one or more amino acid mutations with respect to SEQ ID NOs: 12-24.
  • the CD8 binding agent comprises a targeting moiety comprising an amino acid sequence having one, or two, or three, or four, or five, or six, or seen, or eight, or nine, or ten, or fifteen, or twenty amino acid mutations with respect to SEQ ID NOs: 12-24.
  • the one or more amino acid mutations may be independently selected from substitutions, insertions, deletions, and truncations.
  • the amino acid mutations are amino acid substitutions, and may include conservative and/or non-conservative substitutions.
  • Constant substitutions may be made, for instance, on the basis of similarity in polarity, charge, size, solubility, hydrophobicity, hydrophilicity, and/or the amphipathic nature of the amino acid residues involved.
  • the 20 naturally occurring amino acids can be grouped into the following six standard amino acid groups: (1) hydrophobic: Met, Ala, Val, Leu, He; (2) neutral hydrophilic: Cys, Ser, Thr; Asn, Gin; (3) acidic: Asp, Glu; (4) basic: His, Lys, Arg; (5) residues that influence chain orientation: Gly, Pro; and (6) aromatic: Trp, Tyr, Phe.
  • conservative substitutions are defined as exchanges of an amino acid by another amino acid listed within the same group of the six standard amino acid groups shown above. For example, the exchange of Asp by Glu retains one negative charge in the so modified polypeptide.
  • glycine and proline may be substituted for one another based on their ability to disrupt a-helices.
  • non-conservative substitutions are defined as exchanges of an amino acid by another amino acid listed in a different group of the six standard amino acid groups (1) to (6) shown above.
  • the substitutions may also include non-classical amino acids (e.g. selenocysteine, pyrrolysine, W-formylmethionine ⁇ -alanine, GABA and ⁇ -Aminolevulinic acid, 4-aminobenzoic acid (PABA), D- isomers of the common amino acids, 2,4-diaminobutyric acid, a-amino isobutyric acid, 4-aminobutyric acid, Abu, 2-amino butyric acid, ⁇ -Abu, ⁇ -Ahx, 6-amino hexanoic acid, Aib, 2-amino isobutyric acid, 3-amino propionic acid, ornithine, norleucine, norvaline, hydroxyproline, sarcosme, citrulline, homocitrulline, cysteic acid, t-butylglycine, t- butylalanine, phenylglycine,
  • amino acid mutation may be in the CDRs of the targeting moiety (e.g., the CDR1 , CDR2 or CDR3 regions).
  • amino acid alteration may be in the framework regions (FRs) of the targeting moiety (e.g., the FR1 , FR2, FR3, or FR4 regions).
  • Modification of the amino acid sequences may be achieved using any known technique in the art e.g., site- directed mutagenesis or PCR based mutagenesis. Such techniques are described, for example, in Sambrook ef a/., Molecular Cloning: A Laboratory Manual, Cold Spring Harbor Press, Plainview, N.Y., 1989 and Ausubel et al., Current Protocols in Molecular Biology, John Wiley & Sons, New York, N.Y., 1989.
  • the mutations do not substantially reduce the present CD8 binding agent's capability to specifically bind to CD8. In various embodiments, the mutations do not substantially reduce the present CD8 binding agent's capability to specifically bind to CD8 without functionally modulating CD8.
  • the binding affinity of the CD8 binding agent of the invention for the full-length and/or mature forms and/or isoforms and/or splice variants and/or fragments and/or any other naturally occurring or synthetic analogs, variants, or mutants (including monomeric, dimeric, heterodimeric, multimeric and/or associated forms) of human CD8 a and/or ⁇ chains may be described by the equilibrium dissociation constant (KD).
  • the CD8 binding agent comprises a targeting moiety that binds to the full-length and/or mature forms and/or isoforms and/or splice variants and/or fragments and/or any other naturally occurring or synthetic analogs, variants, or mutants (including monomeric, dimeric, heterodimeric, multimeric and/or associated forms) of human CD8 a and/or ⁇ chains with a KD of less than about 1 uM, about 900 nM, about 800 nM, about 700 nM, about 600 nM, about 500 nM, about 400 nM, about 300 nM, about 200 nM, about 100 nM, about 90 nM, about 80 nM, about 70 nM, about 60 nM, about 50 nM, about 40 nM, about 30 nM, about 20 nM, about 10 nM, or about 5 nM, or about 1 nM.
  • a targeting moiety that binds to the full-length and/or mature
  • the CD8 binding agent comprises a targeting moiety that binds but does not functionally modulate the antigen of interest, i.e., CD8.
  • the targeting moiety of the CD8 binding agent simply targets the antigen but does not substantially functionally modulate the antigen, e.g.it does not substantially inhibit, reduce or neutralize a biological effect that the antigen has.
  • the targeting moiety of the CD8 binding agent binds an epitope that is physically separate from an antigen site that is important for its biological activity (e.g. an antigen's active site).
  • non-functionally modulating (e.g. non-neutralizing) binding finds use in various embodiments of the present invention, including methods in which the present CD8 binding agent is used to directly or indirectly recruit active immune cells to a site of need via an effector antigen.
  • the present CD8 binding agent may be used to directly or indirectly recruit cytotoxic T cells via CD8 to a tumor cell in a method of reducing or eliminating a tumor (e.g. the CD8 binding agent may comprise a targeting moiety having an anti-CD8 antigen recognition domain and a targeting moiety having a recognition domain (e.g. an antigen recognition domain) directed against a tumor antigen or receptor).
  • CD8 signaling is an important piece of the tumor reducing or eliminating effect.
  • the CD8 binding agent of the invention is part of a chimera or fusion with one or more signaling agents. Accordingly, the present invention provides for chimeric or fusion proteins that include, for example, a targeting moiety against CD8 and one or more signaling agents.
  • the signaling agent is modified to have reduced affinity or activity for one or more of its receptors, which allows for attenuation of activity (inclusive of agonism or antagonism) and/or prevents nonspecific signaling or undesirable sequestration of the chimeric or fusion protein.
  • the signaling agent is antagonistic in its wild type form and bears one or more mutations that attenuate its antagonistic activity.
  • the signaling agent is antagonistic due to one or more mutations, e.g. an agonistic signaling agent is converted to an antagonistic signaling agent and, such a converted signaling agent, optionally, also bears one or more mutations that attenuate its antagonistic activity (e.g. as described in WO 2015/007520, the entire contents of which are hereby incorporated by reference).
  • the signaling agent is a modified (e.g. mutant) form of the signaling agent having one or more modifications (e.g. mutations).
  • the mutations allow for the modified signaling agent to have one or more of attenuated activity such as one or more of reduced binding affinity, reduced endogenous activity, and reduced specific bioactivity relative to unmutated, i.e. the wild type form of the signaling agent (e.g. comparing the same signaling agent in a wild type form versus a modified (e.g. mutant) form).
  • the mutations which attenuate or reduce binding or affinity include those mutations which substantially reduce or ablate binding or activity.
  • the mutations which attenuate or reduce binding or affinity are different than those mutations which substantially reduce or ablate binding or activity.
  • the mutations allow for the signaling agent to have improved safety, e.g. reduced systemic toxicity, reduced side effects, and reduced off-target effects relative to unmutated, i.e. wild type, signaling agent (e.g. comparing the same signaling agent in a wild type form versus a modified (e.g. mutant) form).
  • the agent may have improved safety due to one of more modifications, e.g. mutations.
  • improved safety means that the present chimeric protein provides lower toxicity (e.g. systemic toxicity and/or tissue/organ-associated toxicities); and/or lessened or substantially eliminated side effects; and/or increased tolerability, lessened or substantially eliminated adverse events; and/or reduced or substantially eliminated off-target effects; and/or an increased therapeutic window.
  • the signaling agent is modified to have one or more mutations that reduce its binding affinity or activity for one or more of its receptors. In some embodiments, the signaling agent is modified to have one or more mutations that substantially reduce or ablate binding affinity or activity for the receptors.
  • the activity provided by the wild type signaling agent is agonism at the receptor (e.g. activation of a cellular effect at a site of therapy). For example, the wild type signaling agent may activate its receptor.
  • the mutations result in the modified signaling agent to have reduced or ablated activating activity at the receptor. For example, the mutations may result in the modified signaling agent to deliver a reduced activating signal to a target cell or the activating signal could be ablated.
  • the activity provided by the wild type signaling agent is antagonism at the receptor (e.g. blocking or dampening of a cellular effect at a site of therapy).
  • the wild type signaling agent may antagonize or inhibit the receptor.
  • the mutations result in the modified signaling agent to have a reduced or ablated antagonizing activity at the receptor.
  • the mutations may result in the modified signaling agent to deliver a reduced inhibitory signal to a target cell or the inhibitory signal could be ablated.
  • the signaling agent is antagonistic due to one or more mutations, e.g. an agonistic signaling agent is converted to an antagonistic signaling agent (e.g.
  • a converted signaling agent optionally, also bears one or mutations that reduce its binding affinity or activity for one or more of its receptors or that substantially reduce or ablate binding affinity or activity for one or more of its receptors.
  • the reduced affinity or activity at the receptor is restorable by attachment with one or more of the targeting moieties as described herein (e.g., targeting moiety against CD8). In other embodiments, the reduced affinity or activity at the receptor is not substantially restorable by the activity of one or more of the targeting moieties.
  • the chimeric proteins of the present invention reduce off-target effects because their signaling agents have mutations that weaken or ablate binding affinity or activity at a receptor. In various embodiments, this reduction in side effects is observed relative with, for example, the wild type signaling agents.
  • the signaling agent is active on target cells because the targeting moiety(ies) compensates for the missing/insufficient binding (e.g., without limitation and/or avidity) required for substantial activation.
  • the modified signaling agent is substantially inactive en route to the site of therapeutic activity and has its effect substantially on specifically targeted cell types which greatly reduces undesired side effects.
  • the signaling agent may include one or more mutations that attenuate or reduce binding or affinity for one receptor (i.e., a therapeutic receptor) and one or more mutations that substantially reduce or ablate binding or activity at a second receptor. In such embodiments, these mutations may be at the same or at different positions (i.e., the same mutation or multiple mutations). In some embodiments, the mutation(s) that reduce binding and/or activity at one receptor is different than the mutation(s) that substantially reduce or ablate at another receptor. In some embodiments, the mutation(s) that reduce binding and/or activity at one receptor is the same as the mutation(s) that substantially reduce or ablate at another receptor.
  • the present chimeric proteins have a modified signaling agent that has both mutations that attenuate binding and/or activity at a therapeutic receptor and therefore allow for a more controlled, on-target therapeutic effect (e.g. relative wild type signaling agent) and mutations that substantially reduce or ablate binding and/or activity at another receptor and therefore reduce side effects (e.g. relative to wild type signaling agent).
  • the substantial reduction or ablation of binding or activity is not substantially restorable with a targeting moiety (e.g., a targeting moiety against CD8 or any other targeting moiety described herein).
  • the substantial reduction or ablation of binding or activity is restorable with a targeting moiety.
  • substantially reducing or ablating binding or activity at a second receptor also may prevent deleterious effects that are mediated by the other receptor.
  • substantially reducing or ablating binding or activity at the other receptor causes the therapeutic effect to improve as there is a reduced or eliminated sequestration of the therapeutic chimeric proteins away from the site of therapeutic action. For instance, in some embodiments, this obviates the need of high doses of the present chimeric proteins that compensate for loss at the other receptor. Such ability to reduce dose further provides a lower likelihood of side effects.
  • the modified signaling agent comprises one or more mutations that cause the signaling agent to have reduced, substantially reduced, or ablated affinity, e.g. binding (e.g. KD) and/or activation (for instance, when the modified signaling agent is an agonist of its receptor, measurable as, for example, KA and/or EC50) and/or inhibition (for instance, when the modified signaling agent is an antagonist of its receptor, measurable as, for example, Ki and/or IC50), for one or more of its receptors.
  • the reduced affinity at the immumodulating agent's receptor allows for attenuation of activity (inclusive of agonism or antagonism).
  • the modified signaling agent has about 1 %, or about 3%, about 5%, about 10%, about 15%, about 20%, about 25%, about 30%, about 35%, about 40%, about 45%, about 50%, about 60%, about 65%, about 70%, about 75%, about 80%, about 85%, about 90%, about 95%, or about 10%-20%, about 20%-40%, about 50%, about 40%-60%, about 60%-80%, about 80%-100% of the affinity for the receptor relative to the wild type signaling agent.
  • the binding affinity is at least about 2-fold lower, about 3-fold lower, about 4-fold lower, about 5-fold lower, about 6-fold lower, about 7-fold lower, about 8-fold lower, about 9-fold lower, at least about 10-fold lower, at least about 15-fold lower, at least about 20-fold lower, at least about 25-fold lower, at least about 30-fold lower, at least about 35-fold lower, at least about 40-fold lower, at least about 45-fold lower, at least about 50-fold lower, at least about 100-fold lower, at least about 150-fold lower, or about 10-50-fold lower, about 50-100-fold lower, about 100-150-fold lower, about 150-200-fold lower, or more than 200-fold lower relative to the wild type signaling agent.
  • the attenuation or reduction in binding affinity of a modified signaling agent for one receptor is less than the substantial reduction or ablation in affinity for the other receptor. In some embodiments, the attenuation or reduction in binding affinity of a modified signaling agent for one receptor is less than the substantial reduction or ablation in affinity for the other receptor by about 1 %, or about 3%, about 5%, about 10%, about 15%, about 20%, about 25%, about 30%, about 35%, about 40%, about 45%, about 50%, about 60%, about 65%, about 70%, about 75%, about 80%, about 85%, about 90%, or about 95%.
  • the modified signaling agent comprises one or more mutations that reduce the endogenous activity of the signaling agent to about 75%, or about 70%, or about 60%, or about 50%, or about 40%, or about 30%, or about 25%, or about 20%, or about 10%, or about 5%, or about 3%, or about 1 %, e.g., relative to the wild type signaling agent.
  • the modified signaling agent comprises one or more mutations that cause the signaling agent to have reduced affinity for its receptor that is lower than the binding affinity of the targeting moiety(ies) for its(their) receptor(s).
  • this binding affinity differential is between signaling agent/receptor and targeting moiety/receptor on the same cell.
  • this binding affinity differential allows for the signaling agent, e.g. mutated signaling agent, to have localized, on-target effects and to minimize off-target effects that underlie side effects that are observed with wild type signaling agent.
  • this binding affinity is at least about 2-fold, or at least about 5-fold, or at least about 10-fold, or at least about 15-fold lower, or at least about 25-fold, or at least about 50-fold lower, or at least about 100-fold, or at least about 150- fold.
  • Receptor binding activity may be measured using methods known in the art. For example, affinity and/or binding activity may be assessed by Scatchard plot analysis and computer-fitting of binding data (e.g. Scatchard, 1949) or by reflectometric interference spectroscopy under flow through conditions, as described by Brecht ef a/. (1993), the entire contents of all of which are hereby incorporated by reference.
  • the signaling agent is an immune-modulating agent, e.g. one or more of an interleukin, interferon, and tumor necrosis factor.
  • the signaling agent is an interleukin or a modified interleukin, including for example IL-1 ; IL-2; IL-3; IL-4; IL-5; IL-6; IL-7; IL-8; IL-9; IL-10; IL-11 ; IL-12; IL-13; IL-14; IL-15; IL-16; IL-17; IL-18; IL-19; IL-20; IL-21 ; IL-22; IL-23; IL-24; IL-25; IL-26; IL-27; IL-28; IL-29; IL-30; IL-31 ; IL-32; IL-33; IL-35; IL-36 or a fragment, variant, analogue, or family-member thereof.
  • Interleukins are a group of multi- functional cytokines synthesized by lymphocytes, monocytes, and macrophages.
  • Known functions include stimulating proliferation of immune cells (e.g., T helper cells, B cells, eosinophils, and lymphocytes), chemotaxis of neutrophils and T lymphocytes, and/or inhibition of interferons.
  • Interleukin activity can be determined using assays known in the art: Matthews ef a/., in Lymphokines and Interferens: A Practical Approach, Clemens ef a/., eds, IRL Press, Washington, D.C. 1987, pp. 221-225; and Orencole & Dinarello (1989) Cytokine 1 , 14-20.
  • the signaling agent is an interferon or a modified version of an interferon such as interferon types I, II, and III.
  • interferon types I, II, and III Illustrative interferons, including for example, interferon-a-1 , 2, 4, 5, 6, 7, 8, 10, 13, 14, 16, 17, and 21 , interferon- ⁇ and interferon- ⁇ , interferon ⁇ , interferon ⁇ , interferon ⁇ , and interferon TO.
  • the signaling agent is a tumor necrosis factor (TNF) or a modified version of a tumor necrosis factor (TNF) or a protein in the TNF family, including but not limited to, TNF-a, TNF- ⁇ , LT- ⁇ , CD40L, CD27L, CD30L, FASL, 4-1 BBL, OX40L, and TRAIL.
  • TNF tumor necrosis factor
  • TNF tumor necrosis factor
  • TNF tumor necrosis factor
  • TNF tumor necrosis factor
  • TNF tumor necrosis factor
  • a protein in the TNF family including but not limited to, TNF-a, TNF- ⁇ , LT- ⁇ , CD40L, CD27L, CD30L, FASL, 4-1 BBL, OX40L, and TRAIL.
  • the modified signaling agent comprises an amino acid sequence that has at least about 60%, or at least about 61 %, or at least about 62%, or at least about 63%, or at least about 64%, or at least about 65%, or at least about 66%, or at least about 67%, or at least about 68%, or at least about 69%, or at least about 70%, or at least about 71 %, or at least about 72%, or at least about 73%, or at least about 74%, or at least about 75%, or at least about 76%, or at least about 77%, or at least about 78%, or at least about 79%, or at least about 80%, or at least about 81 %, or at least about 82%, or at least about 83%, or at least about 84%, or at least about 85%, or at least about 86%, or at least about 87%, or at least about 88%, or at least about 89%, or at least about 90%, or at least about 91 %, or at least about 92%, or
  • the modified signaling agent comprises an amino acid sequence that has at least about 60%, or at least about 61 %, or at least about 62%, or at least about 63%, or at least about 64%, or at least about 65%, or at least about 66%, or at least about 67%, or at least about 68%, or at least about 69%, or at least about 70%, or at least about 71 %, or at least about 72%, or at least about 73%, or at least about 74%, or at least about 75%, or at least about 76%, or at least about 77%, or at least about 78%, or at least about 79%, or at least about 80%, or at least about 81 %, or at least about 82%, or at least about 83%, or at least about 84%, or at least about 85%, or at least about 86%, or at least about 87%, or at least about 88%, or at least about 89%, or at least about 90%, or at least about 91 %, or at least about 92%, or at least about
  • the modified signaling agent comprises an amino acid sequence having one or more amino acid mutations.
  • the one or more amino acid mutations may be independently selected from substitutions, insertions, deletions, and truncations.
  • the amino acid mutations are amino acid substitutions, and may include conservative and/or non-conservative substitutions, as described elsewhere herein.
  • substitutions may also include non-classical amino acids as described elsewhere herein.
  • the modified signaling agents bear mutations that affect affinity and/or activity at one or more receptors.
  • the receptors of any signaling agents, as described herein, are known in the art.
  • Illustrative mutations which provide reduced affinity and/or activity (e.g. agonistic) at a receptor are found in WO 2013/107791 ⁇ e.g. with regard to interferons), WO 2015/007542 ⁇ e.g. with regard to interleukins), and WO 2015/007903 ⁇ e.g. with regard to TNF), the entire contents of each of which are hereby incorporated by reference.
  • Illustrative mutations which provide reduced affinity and/or activity ⁇ e.g. antagonistic) at a receptor are found in WO 2015/007520, the entire contents of which are hereby incorporated by reference.
  • the modified signaling agent is interferon a.
  • the modified IFN-a agent has reduced affinity and/or activity for the IFN- ⁇ / ⁇ receptor (IFNAR), i.e., IFNAR1 and/or IFNAR2 chains.
  • the modified IFN-a agent has substantially reduced or ablated affinity and/or activity for the IFN- ⁇ / ⁇ receptor (IFNAR), i.e., IFNAR1 and/or IFNAR2 chains.
  • the modified signaling agent is the allelic form IFN-a2a having the amino acid sequence of:
  • IFN-a2a SEQ ID NO:25
  • the modified signaling agent is the allelic form IFN-a2b having the amino acid sequence of (which differs from IFN-a2a at amino acid position 23):
  • IFN-a2b SEQ ID NO:26
  • said IFN-a2 mutant (IFN-a2a or IFN-a2b) is mutated at one or more amino acids at positions 144-154, such as amino acid positions 148, 149 and/or 153.
  • the IFN-a2 mutant comprises one or more mutations selected from L153A, R149A, and M148A. Such mutants are described, for example, in WO2013/107791 and Piehler ef a/., (2000) J. Biol. Chem, 275:40425-33, the entire contents of all of which are hereby incorporated by reference.
  • the IFN-a2 mutants have reduced affinity and/or activity for IFNAR1.
  • the IFN-a2 mutant comprises one or more mutations selected from F64A, N65A, T69A, L80A, Y85A, and Y89A, as described in WO2010/030671 , the entire contents of which is hereby incorporated by reference.
  • the IFN-a2 mutant comprises one or more mutations selected from K133A, R144A, R149A, and L153A as described in WO2008/124086, the entire contents of which is hereby incorporated by reference.
  • the IFN-a2 mutant comprises one or more mutations selected from R120E and R120E/K121 E, as described in WO2015/007520 and WO2010/030671 , the entire contents of which are hereby incorporated by reference.
  • said IFN-a2 mutant antagonizes wildtype IFN-a2 activity.
  • said mutant IFN-a2 has reduced affinity and/or activity for IFNAR1 while affinity and/or activity of IFNAR2 is retained.
  • the human IFN-a2 mutant comprises (1) one or more mutations selected from R120E and R120E/K121 E, which, without wishing to be bound by theory, create an antagonistic effect and (2) one or more mutations selected from K133A, R144A, R149A, and L153A, which, without wishing to be bound by theory, allow for an attenuated effect at, for example, IFNAR2.
  • the human IFN-a2 mutant comprises R120E and L153A.
  • the human IFN-a2 mutant comprises one or more mutations selected from, L15A, A19W, R22A, R23A, L26A, F27A, L30A, L30V, K31A, D32A, R33K, R33A, R33Q, H34A, D35A, Q40A, D114R, L117A, R120A, R125A , K134A, R144A, A145G, A145M, M148A, R149A, S152A, L153A, and N156A as disclosed in WO 2013/059885, the entire disclosures of which are hereby incorporated by reference.
  • the human IFN-a2 mutant comprises the mutations H57Y, E58N, Q61 S, and/or L30A as disclosed in WO 2013/059885. In some embodiments, the human IFN-a2 mutant comprises the mutations H57Y, E58N, Q61 S, and/or R33A as disclosed in WO 2013/059885. In some embodiments, the human IFN-a2 mutant comprises the mutations H57Y, E58N, Q61 S, and/or M148A as disclosed in WO 2013/059885. In some embodiments, the human IFN-a2 mutant comprises the mutations H57Y, E58N, Q61 S, and/or L153A as disclosed in WO 2013/059885.
  • the human IFN-a2 mutant comprises the mutations N65A, L80A, Y85A, and/or Y89A as disclosed in WO 2013/059885. In some embodiments, the human IFN-a2 mutant comprises the mutations N65A, L80A, Y85A, Y89A, and/or D114A as disclosed in WO 2013/059885.
  • the modified signaling agent is interferon ⁇ .
  • the modified interferon ⁇ agent has reduced affinity and/or activity for the IFN- ⁇ / ⁇ receptor (IFNAR), i.e., IFNAR1 and/or IFNAR2 chains.
  • the modified interferon ⁇ agent has substantially reduced or ablated affinity and/or activity for the IFN- ⁇ / ⁇ receptor (IFNAR), i.e., IFNAR1 and/or IFNAR2 chains.
  • the modified signaling agent is interferon ⁇ .
  • the modified interferon ⁇ agent has reduced affinity and/or activity for the interferon-gamma receptor (IFNGR), i.e., IFNGR1 and IFNGR2 chains.
  • the modified interferon ⁇ agent has substantially reduced or ablated affinity and/or activity for the interferon-gamma receptor (IFNGR), i.e., IFNGR1 and/or IFNGR2 chains.
  • the modified signaling agent is TNF-a.
  • TNF is a pleiotropic cytokine with many diverse functions, including regulation of cell growth, differentiation, apoptosis, tumorigenesis, viral replication, autoimmunity, immune cell functions and trafficking, inflammation, and septic shock. It binds to two distinct membrane receptors on target cells: TNFR1 (p55) and TNFR2 (p75).
  • TNFR1 exhibits a very broad expression pattern whereas TNFR2 is expressed preferentially on certain populations of lymphocytes, Tregs, endothelial cells, certain neurons, microglia, cardiac myocytes and mesenchymal stem cells. Very distinct biological pathways are activated in response to receptor activation, although there is also some overlap.
  • TNFR1 signaling is associated with induction of apoptosis (cell death) and TNFR2 signaling is associated with activation of cell survival signals (e.g. activation of NFkB pathway).
  • Administration of TNF is systemically toxic, and this is largely due to TNFR1 engagement.
  • activation of TNFR2 is also associated with a broad range of activities and, as with TNFR1 , in the context of developing TNF based therapeutics, control over TNF targeting and activity is important.
  • the modified signaling agent has reduced affinity and/or activity for TNFR1 and/or TNFR2. In some embodiments, the modified signaling agent has substantially reduced or ablated affinity and/or activity for TNFR1 and/or TNFR2.
  • TNFR1 is expressed in most tissues, and is involved in cell death signaling while, by contrast, TNFR2 is involved in cell survival signaling. Accordingly, in embodiments directed to methods of treating cancer, the modified signaling agent has reduced affinity and/or activity for TNFR1 and/or substantially reduced or ablated affinity and/or activity for TNFR2.
  • the chimeric proteins may be targeted to a cell for which apoptosis is desired, e.g.
  • the modified signaling agent has reduced affinity and/or activity for TNFR2 and/or substantially reduced or ablated affinity and/or activity for TNFR1.
  • the present chimeric proteins in some embodiments, comprise modified TNF-a agent that allows of favoring either death or survival signals.
  • the chimeric protein has a modified TNF having reduced affinity and/or activity for TNFR1 and/or substantially reduced or ablated affinity and/or activity for TNFR2.
  • a chimera in some embodiments, is a more potent inducer of apoptosis as compared to a wild type TNF and/or a chimera bearing only mutation(s) causing reduced affinity and/or activity for TNFR1.
  • Such a chimera finds use in inducing tumor cell death or a tumor vasculature endothelial cell death (e.g. in the treatment of cancers).
  • these chimeras avoid or reduce activation of T reg cells via TNFR2, for example, thus further supporting TNFR1 -mediated antitumor activity in vivo.
  • the chimeric protein has a modified TNF having reduced affinity and/or activity for TNFR2 and/or substantially reduced or ablated affinity and/or activity for TNFR1.
  • a chimera in some embodiments, is a more potent activator of cell survival in some cell types, which may be a specific therapeutic objective in various disease settings, including without limitation, stimulation of neurogenesis.
  • a TNFR2- favoring chimeras also are useful in the treatment of autoimmune diseases (e.g. Crohn's, diabetes, MS, colitis etc. and many others described herein).
  • the chimera is targeted to auto-reactive T cells.
  • the chimera promotes T reg cell activation and indirect suppression of cytotoxic T cells.
  • the chimera causes the death of auto-reactive T cells, e.g. by activation of TNFR2 and/or avoidance of TNFR1 (e.g. a modified TNF having reduced affinity and/or activity for TNFR2 and/or substantially reduced or ablated affinity and/or activity for TNFR1).
  • TNFR1 e.g. a modified TNF having reduced affinity and/or activity for TNFR2 and/or substantially reduced or ablated affinity and/or activity for TNFR1
  • these auto-reactive T cells have their apoptosis/survival signals altered e.g. by NFkB pathway activity/signaling alterations.
  • the chimera causes the death of autoreactive T cells having lesions or modifications in the NFkB pathway, which underlie an imbalance of their cell death (apoptosis)/survival signaling properties and, optionally, altered susceptibility to certain death-inducing signals (e.g., TNFR2 activation).
  • a TNFR2 based chimera has additional therapeutic applications in diseases, including various autoimmune diseases, heart disease, de-myelinating and neurodegenerative disorders, and infectious disease, among others.
  • the wild type TNF-a has the amino acid sequence of:
  • TNF-a SEQ ID NO:27
  • the modified TNF-a agent has mutations at one or more amino acid positions 29, 31 , 32, 84, 85, 86, 87, 88, 89, 145, 146 and 147 which produces a modified TNF-a with reduced receptor binding affinity. See, for example, U.S. Patent No. 7,993,636, the entire contents of which are hereby incorporated by reference.
  • the modified human TNF-a moiety has mutations at one or more amino acid positions R32, N34, Q67, H73, L75, T77, S86, Y87, V91 , I97, T105, P106, A109, P113, Y115, E127, N137, D143, and A145, as described, for example, in WO/2015/007903, the entire contents of which is hereby incorporated by reference (numbering according to the human TNF sequence, Genbank accession number BAG70306, version BAG70306.1 Gl: 197692685).
  • the modified human TNF-a moiety has substitution mutations selected from R32G, N34G, Q67G, H73G, L75G, L75A, L75S, T77A, S86G, Y87Q, Y87L, Y87A, Y87F, V91G, V91A, I97A, I97Q, I97S, T105G, P106G, A109Y, P113G, Y115G, Y115A, E127G, N137G, D143N, A145G and A145T.
  • the human TNF-a moiety has a mutation selected from Y87Q, Y87L, Y87A, and Y87F.
  • the human TNF-a moiety has a mutation selected from I97A, I97Q, and I97S. In a further embodiment, the human TNF-a moiety has a mutation selected from Y115A and Y115G. In some embodiments, the modified TNF-a agent has one or more mutations selected from N39Y, S147Y, and Y87H, as described in WO2008/124086, the entire contents of which is hereby incorporated by reference.
  • the modified human TNF-a moiety has mutations that provide receptor selectivity as described in PCT/I B2016/001668, the entire contents of which are hereby incorporated by reference.
  • the mutations to TNF are TNF-R1 selective.
  • the mutations to TNF which are TNF-R1 selective are at one or more of positions R32, S86, and E146.
  • the mutations to TNF which are TNF-R1 selective are one or more of R32W, S86T, and E146K.
  • the mutations to TNF which are TNF-R1 selective are one or more of R32W, R32W/S86T, R32W/E146K and E146K.
  • the mutations to TNF are TNF-R2 selective. In some embodiments, the mutations to TNF which are TNF-R2 selective are at one or more of positions A145, E146, and S147. In some embodiments, the mutations to TNF which are TNF-R2 selective are one or more of A145T, A145R, E146D, and S147D. In some embodiments, the mutations to TNF which are TNF-R2 selective are one or more of A145R, A145T/S147D, and A145T/E146D/S147D.
  • the modified signaling agent is TNF- ⁇ .
  • TNF- ⁇ can form a homotrimer or a heterotrimer with LT- ⁇ ( ⁇ _ ⁇ - ⁇ 1 ⁇ 2).
  • the modified signaling agent has substantially reduced or ablated affinity and/or activity for TNFR1 and/or TNFR2 and/or herpes virus entry mediator (HEVM) and/or LT ⁇ R.
  • HEVM herpes virus entry mediator
  • the wild type TNF- ⁇ has the amino acid sequence of:
  • TNF-beta SEQ ID NO:28
  • the modified TNF- ⁇ agent may comprise mutations at one or more amino acids at positions 106-113, which produce a modified TNF- ⁇ with reduced receptor binding affinity to TNFR2.
  • the modified signaling agent has one or more substitution mutations at amino acid positions 106- 113.
  • the substitution mutations are selected from Q107E, Q107D, S106E, S106D, Q107R, Q107N, Q107E/S106E, Q107E/S106D, Q107D/S106E, and Q107D/S106D.
  • the modified signaling agent has an insertion of about 1 to about 3 amino acids at positions 106-113.
  • the modified agent is a TNF family member (e.g. TNF-alpha, TNF-beta) which can be a single chain trimeric version as described in WO 2015/007903, the entire contents of which are incorporated by reference.
  • TNF family member e.g. TNF-alpha, TNF-beta
  • the modified agent is a TNF family member (e.g. TNF-alpha, TNF-beta) which has reduced affinity and/or activity, i.e. antagonistic activity (e.g. natural antagonistic activity or antagonistic activity that is the result of one or more mutations, see, e.g., WO 2015/007520, the entire contents of which are hereby incorporated by reference) at TNFR1.
  • the modified agent is a TNF family member (e.g. TNF-alpha, TNF-beta) which also, optionally, has substantially reduced or ablated affinity and/or activity for TNFR2.
  • the modified agent is a TNF family member (e.g.
  • TNF-alpha, TNF-beta which has reduced affinity and/or activity, i.e. antagonistic activity (e.g. natural antagonistic activity or antagonistic activity that is the result of one or more mutations, see, e.g., WO 2015/007520, the entire contents of which are hereby incorporated by reference) at TNFR2.
  • the modified agent is a TNF family member (e.g. TNF-alpha, TNF-beta) which also, optionally, has substantially reduced or ablated affinity and/or activity for TNFR1.
  • the constructs of such embodiments find use in, for example, methods of dampening TNF response in a cell specific manner.
  • the antagonistic TNF family member e.g. TNF-alpha, TNF-beta
  • the antagonistic TNF family member is a single chain trimeric version as described in WO 2015/007903.
  • the modified signaling agent is TRAIL.
  • the modified TRAIL agent has reduced affinity and/or activity for DR4 (TRAIL-RI) and/or DR5 (TRAIL-RII) and/or DcR1 and/or DcR2.
  • the modified TRAIL agent has substantially reduced or ablated affinity and/or activity for DR4 (TRAIL-RI) and/or DR5 (TRAIL-RII) and/or DcR1 and/or DcR2.
  • the wild type TRAIL has the amino acid sequence of:
  • the modified TRAIL agent may comprise a mutation at amino acid positions T127-R132, E144-R149, E155-H161 , Y189-Y209, T214-1220,K224-A226, W231 , E236-L239, E249-K251 , T261-H264 and H270-E271 (Numbering based on the human sequence, Genbank accession number NP _003801 , version 10 NP _003801.1 , GI: 4507593; see above).
  • the modified signaling agent is an interleukin. In an embodiment, the modified signaling agent is IL-1. In an embodiment, the modified signaling agent is IL-1a or IL-1 ⁇ . In some embodiments, the modified signaling agent has reduced affinity and/or activity for IL-1 R1 and/or IL-1 RAcP. In some embodiments, the modified signaling agent has substantially reduced or ablated affinity and/or activity for IL-1 R1 and/or IL-1 RAcP. In some embodiments, the modified signaling agent has reduced affinity and/or activity for IL-1 R2. In some embodiments, the modified signaling agent has substantially reduced or ablated affinity and/or activity for IL-1 R2. For instance, in some embodiments, the present modified IL-1 agents avoid interaction at IL-1 R2 and therefore substantially reduce its function as a decoy and/or sink for therapeutic agents. In an embodiment, the wild type IL-1 ⁇ has the amino acid sequence of:
  • IL-1 beta (mature form, wild type) (SEQ ID NO:30)
  • IL-1 is a proinflammatory cytokine and an important immune system regulator. It is a potent activator of CD4 T cell responses, increases proportion of Th17 cells and expansion of IFNy and IL-4 producing cells. IL-1 is also a potent regulator of CD8 + T cells, enhancing antigen-specific CD8 + T cell expansion, differentiation, migration to periphery and memory.
  • IL-1 receptors comprise IL-1 R1 and IL-1 R2. Binding to and signaling through the IL-1 R1 constitutes the mechanism whereby IL-1 mediates many of its biological (and pathological) activities.
  • IL1 -R2 can function as a decoy receptor, thereby reducing IL-1 availability for interaction and signaling through the IL-1 R1.
  • the modified IL-1 has reduced affinity and/or activity (e.g. agonistic activity) for IL-1 R1. In some embodiments, the modified IL-1 has substantially reduced or ablated affinity and/or activity for IL-1 R2. In such embodiments, there is restorable IL-1/ IL-1 R1 signaling and prevention of loss of therapeutic chimeras at IL- R2 and therefore a reduction in dose of IL-1 that is required (e.g. relative to wild type or a chimera bearing only an attenuation mutation for IL-R1). Such constructs find use in, for example, methods of treating cancer, including, for example, stimulating the immune system to mount an anti-cancer response.
  • the modified IL-1 has reduced affinity and/or activity (e.g. antagonistic activity, e.g. natural antagonistic activity or antagonistic activity that is the result of one or more mutations, see, e.g., WO 2015/007520, the entire contents of which are hereby incorporated by reference) for IL-1 R1.
  • the modified IL-1 has substantially reduced or ablated affinity and/or activity for IL-1 R2.
  • there is the IL-1/ IL-1 R1 signaling is not restorable and prevention of loss of therapeutic chimeras at IL-R2 and therefore a reduction in dose of IL-1 that is required (e.g. relative to wild type or a chimera bearing only an attenuation mutation for IL-R1).
  • Such constructs find use in, for example, methods of treating autoimmune diseases, including, for example, suppressing the immune system.
  • the modified signaling agent has a deletion of amino acids 52-54 which produces a modified human IL-1 ⁇ with reduced binding affinity for type I IL-1 R and reduced biological activity. See, for example, WO 1994/000491 , the entire contents of which are hereby incorporated by reference.
  • the modified human IL-1 ⁇ has one or more substitution mutations selected from A117G/P118G, R120X, L122A, T125G/L126G, R127G, Q130X, Q131 G, K132A, S137G/Q138Y, L145G, H146X, L145A/L147A, Q148X, Q148G/Q150G, Q150G/D151A, M152G, F162A, F162A/Q164E, F166A, Q164E/E167K, N169G/D170G, I172A, V174A, K208E, K209X, K209A/K210A, K219X, E221X, E221 S/N224A, N224S/K225S, E244K, N245Q (where X can be any change in amino acid, e.g., a non-conservative change), which exhibit reduced binding to IL-1 R, as described, for example, in WO2015/
  • the modified human IL-1 ⁇ may have one or more mutations selected from R120A, R120G, Q130A, Q130W, H146A, H146G, H146E, H146N, H146R, Q148E, Q148G, Q148L, K209A, K209D, K219S, K219Q, E221 S and E221 K.
  • the modified human IL-1 ⁇ comprises the mutations Q131 G and Q148G.
  • the modified human IL-1 ⁇ comprises the mutations Q148G and K208E.
  • the modified human IL-1 ⁇ comprises the mutations R120G and Q131 G.
  • the modified human IL-1 ⁇ comprises the mutations R120G and H146A. In an embodiment, the modified human IL-1 ⁇ comprises the mutations R120G and H146N. In an embodiment, the modified human IL-1 ⁇ comprises the mutations R120G and H146R. In an embodiment, the modified human IL-1 ⁇ comprises the mutations R120G and H146E. In an embodiment, the modified human IL-1 ⁇ comprises the mutations R120G and H146G. In an embodiment, the modified human IL-1 ⁇ comprises the mutations R120G and K208E. In an embodiment, the modified human IL-1 ⁇ comprises the mutations R120G, F162A, and Q164E.
  • the modified signaling agent is IL-2.
  • the modified signaling agent has reduced affinity and/or activity for IL-2Ra and/or ⁇ L-2R and/or IL-2Ry.
  • the modified signaling agent has reduced affinity and/or activity for ⁇ L-2R and/or IL-2Ry.
  • the modified signaling agent has substantially reduced or ablated affinity and/or activity for IL-2Ra. Such embodiments may be relevant for treatment of cancer, for instance when the modified IL-2 is agonistic at ⁇ L-2R and/or IL-2Ry.
  • the present constructs may favor attenuated activation of CD8 + T cells (which can provide an anti-tumor effect), which have IL2 receptors ⁇ and ⁇ and disfavor T reg s (which can provide an immune suppressive, pro- tumor effect), which have IL2 receptors ⁇ , ⁇ , and ⁇ .
  • the preferences for ⁇ L-2R and/or IL-2Ry over IL-2Ra avoid IL-2 side effects such as pulmonary edema.
  • IL-2-based chimeras are useful for the treatment of autoimmune diseases, for instance when the modified IL-2 is antagonistic (e.g.
  • the present constructs may favor attenuated suppression of CD8 + T cells (and therefore dampen the immune response), which have IL2 receptors ⁇ and ⁇ and disfavor T reg s which have IL2 receptors ⁇ , ⁇ , and ⁇ .
  • the chimeras bearing IL-2 favor the activation of T reg s, and therefore immune suppression, and activation of disfavor of CD8 + T cells.
  • these constructs find use in the treatment of diseases or diseases that would benefit from immune suppression, e.g. autoimmune disorders.
  • the chimeric protein has targeting moieties as described herein directed to CD8 + T cells as well as a modified IL-2 agent having reduced affinity and/or activity for ⁇ L-2R and/or IL-2Ry and/or substantially reduced or ablated affinity and/or activity for IL-2Ra.
  • these constructs provide targeted CD8 + T cell activity and are generally inactive (or have substantially reduced activity) towards T reg cells.
  • such constructs have enhanced immune stimulatory effect compared to wild type IL-2 (e.g., without wishing to be bound by theory, by not stimulating Tregs), whilst eliminating or reducing the systemic toxicity associated with IL-2.
  • the wild type IL-2 has the amino acid sequence of:
  • IL-2 mature form, wild type (SEQ ID NO:31)
  • the modified IL-2 agent has one or more mutations at amino acids L72 (L72G, L72A, L72S, L72T, L72Q, L72E, L72N, L72D, L72R, or L72K), F42 (F42A, F42G, F42S, F42T, F42Q, F42E, F42N, F42D, F42R, or F42K) and Y45 (Y45A, Y45G, Y45S, Y45T, Y45Q, Y45E, Y45N, Y45D, Y45R or Y45K).
  • modified IL-2 agents have reduced affinity for the high- affinity IL-2 receptor and preserves affinity to the intermediate-affinity IL-2 receptor, as compared to the wild-type IL-2. See, for example, US Patent Publication No. 2012/0244112, the entire contents of which are hereby incorporated by reference.
  • the modified signaling agent is IL-3.
  • the modified signaling agent has reduced affinity and/or activity for the IL-3 receptor, which is a heterodimer with a unique alpha chain paired with the common beta (beta c or CD131) subunit.
  • the modified signaling agent has substantially reduced or ablated affinity and/or activity for the IL-3 receptor, which is a heterodimer with a unique alpha chain paired with the common beta (beta c or CD131) subunit.
  • the modified signaling agent is IL-4.
  • the modified signaling agent has reduced affinity and/or activity for type 1 and/or type 2 IL-4 receptors.
  • the modified signaling agent has substantially reduced or ablated affinity and/or activity for type 1 and/or type 2 IL-4 receptors.
  • Type 1 IL-4 receptors are composed of the IL-4Ra subunit with a common ⁇ chain and specifically bind IL-4.
  • Type 2 IL-4 receptors include an IL-4Ra subunit bound to a different subunit known as IL-13Ra1.
  • the modified signaling agent has substantially reduced or ablated affinity and/or activity the type 2 IL-4 receptors.
  • the wild type IL-4 has the amino acid sequence of:
  • IL-4 mature form, wild type (SEQ ID NO:32)
  • the modified IL-4 agent has one or more mutations at amino acids R121 (R121A, R121 D, R121 E, R121 F, R121 H, R121 I, R121 K, R121 N, R121 P, R121T, R121W), E122 (E122F), Y124 (Y124A, Y124Q, Y124R, Y124S, Y124T) and S125 (S125A).
  • E122 (E122F) E122 (E122F)
  • Y124 Y124A, Y124Q, Y124R, Y124S, Y124T
  • S125A S125A
  • the modified signaling agent is IL-6.
  • IL-6 signals through a cell-surface type I cytokine receptor complex including the ligand-binding IL-6R chain (CD126), and the signal-transducing component gp130.
  • IL-6 may also bind to a soluble form of IL-6R (slL-6R), which is the extracellular portion of IL-6R.
  • slL-6R soluble form of IL-6R
  • the sIL- 6R/IL-6 complex may be involved in neurites outgrowth and survival of neurons and, hence, may be important in nerve regeneration through remyelination.
  • the modified signaling agent has reduced affinity and/or activity for IL-6R/gp130 and/or slL-6R.
  • the modified signaling agent has substantially reduced or ablated affinity and/or activity for IL-6R/gp130 and/or slL-6R.
  • the wild type IL-6 has the amino acid sequence of:
  • IL-6 mature form, wild type (SEQ ID NO:33)
  • the modified signaling agent has one or more mutations at amino acids 58, 160, 163, 171 or 177.
  • these modified IL-6 agents exhibit reduced binding affinity to IL-6Ralpha and reduced biological activity. See, for example, WO 97/10338, the entire contents of which are hereby incorporated by reference.
  • the modified signaling agent is IL-10.
  • the modified signaling agent has reduced affinity and/or activity for IL-10 receptor-1 and IL-10 receptor-2.
  • the modified signaling agent has substantially reduced affinity and/or activity for IL-10 receptor-1 and IL-10 receptor-2
  • the modified signaling agent is IL-11.
  • the modified signaling agent has reduced affinity and/or activity for IL-11 Ra and/or IL-11 R and/or gp130.
  • the modified signaling agent has substantially reduced affinity and/or activity for IL-11 Ra and/or IL-11 R and/or gp130.
  • the modified signaling agent is IL-12.
  • the modified signaling agent has reduced affinity and/or activity for IL-12Rpi and/or IL-12R 2.
  • the modified signaling agent has substantially reduced or ablated affinity and/or activity for IL-12Rpi and/or IL-12R 2.
  • the modified signaling agent is IL-13.
  • the modified signaling agent has reduced affinity and/or activity for the IL-4 receptor (IL-4Ra) and IL-13Ra1.
  • the modified signaling agent has substantially reduced or ablated affinity and/or activity for IL-4 receptor (IL-4Ra) or IL-13Ra1.
  • the wild type IL-13 has the amino acid sequence of:
  • IL-13 mature form, wild type (SEQ ID NO:34)
  • the modified IL-13 agent has one or more mutations at amino acids 13, 16, 17, 66, 69, 99, 102, 104, 105, 106, 107, 108, 109, 112, 113 and 114. Without wishing to be bound by theory, it is believed that these modified IL-13 agents exhibit reduced biological activity. See, for example, WO 2002/018422, the entire contents of which are hereby incorporated by reference.
  • the modified signaling agent is IL-18. In some embodiments, the modified signaling agent has reduced affinity and/or activity for IL-18Ra and/or IL-18 ⁇ . In some embodiments, the modified signaling agent has substantially reduced or ablated affinity and/or activity for IL-18Ra and/or IL-18 ⁇ . In some embodiments, the modified signaling agent has substantially reduced or ablated affinity and/or activity for IL-18Ra type II, which is an isoform of IL-18Ra that lacks the TIR domain required for signaling.
  • the wild type IL-18 has the amino acid sequence of:
  • the modified IL-18 agent may comprise one or more mutations in amino acids or amino acid regions selected from Y37-K44, R49-Q54, D59-R63, E67-C74, R80, M87-A97, N 127-K129, Q139-M149, K165-K171 , R183 and Q190-N191 , as described in WO/2015/007542, the entire contents of which are hereby incorporated by reference (numbering based on the human IL-18 sequence, Genbank accession number AAV38697, version AAV38697.1 , Gl: 54696650).
  • the modified signaling agent is IL-33.
  • the modified signaling agent has reduced affinity and/or activity for the ST-2 receptor and IL-1 RAcP.
  • the modified signaling agent has substantially reduced or ablated affinity and/or activity for the ST-2 receptor and IL-1 RAcP.
  • the wild type IL-33 has the amino acid sequence of:
  • the modified IL-33 agent may comprise one or more mutations in amino acids or amino acid regions selected from I113-Y122, S127-E139, E144-D157, Y163-M183, E200, Q215, L220-C227 and T260- E269, as described in WO/2015/007542, the entire contents of which are hereby incorporated by reference (numbering based on the human sequence, Genbank accession number NP_254274, version NP_254274.1 , Gl:15559209).
  • the present chimeric protein has (i) a CD8 binding agent and (ii) a targeting moiety which is directed against a tumor cell, along with any of the modified (e.g. mutant) form signaling agents described herein.
  • the present chimeric protein has a targeting moiety directed against CD8 on T cells and a second targeting moiety directed against PD-L1 or PD-L2 on tumor cells.
  • the signaling agent is a toxin or toxic enzyme.
  • the toxin or toxic enzyme is derived from plants and bacteria.
  • Illustrative toxins or toxic enzymes include, but are not limited to, the diphtheria toxin, Pseudomonas toxin, anthrax toxin, ribosome-inactivating proteins (RIPs) such as ricin and saporin, modeccin, abrin, gelonin, and poke weed antiviral protein. Additional toxins include those disclosed in Mathew et al., (2009) Cancer Sci 100(8): 1359-65, the entire disclosures are hereby incorporated by reference.
  • the chimeric proteins of the invention may be utilized to induce cell death in cell-type specific manner.
  • the toxin may be modified, e.g. mutated, to reduce affinity and/or activity of the toxin for an attenuated effect, as described with other signaling agents herein.
  • the CD8 binding agent of the invention is part of a chimera or fusion with one or more signaling agents as described herein and/or one or more additional targeting moieties. Accordingly, the present invention provides for chimeric or fusion proteins that include one or more signaling agents and a targeting moiety against CD8 and/or one or more additional targeting moieties.
  • the CD8 binding agent of the invention is multispecific, i.e., the CD8 binding agent comprises two or more targeting moieties having recognition domains that recognize and bind two or more targets, e.g. antigens, or receptors, or epitopes.
  • the CD8 binding agent of the invention may comprise two more targeting moieties having recognition domains that recognize and bind two or more epitopes on the same antigen or on different antigens.
  • such multi-specific CD8 binding agents exhibit advantageous properties such as increased avidity and/or improved selectivity.
  • the CD8 binding agent of the invention comprises two targeting moieties and is bispecific, i.e., binds and recognizes two epitopes on the same antigen or on different antigens.
  • the multispecific CD8 binding agent of the invention comprises two or more targeting moieties with each targeting moiety being an antibody or an antibody derivative as described herein.
  • the multispecific CD8 binding agent of the invention comprises at least one VHH comprising an antigen recognition domain against CD8 and one antibody or antibody derivative comprising an antigen recognition domain against a tumor antigen.
  • the present multispecific CD8 binding agents have two or more targeting moieties that target different antigens or receptors, and one targeting moiety may be attenuated for its antigen or receptor, e.g. the targeting moiety binds its antigen or receptor with a low affinity or avidity (including, for example, at an affinity or avidity that is less than the affinity or avidity the other targeting moiety has for its for its antigen or receptor, for instance the difference between the binding affinities may be about 10-fold, or 25-fold, or 50-fold, or 100-fold, or 300-fold, or 500-fold, or 1000-fold, or 5000-fold; for instance the lower affinity or avidity targeting moiety may bind its antigen or receptor at a KD in the mid- to high-nM or low- to mid- ⁇ range while the higher affinity or avidity targeting moiety may bind its antigen or receptor at a KD in the mid- to high-pM or low- to mid-nM range).
  • a low affinity or avidity including, for example,
  • the present multispecific CD8 binding agents comprises an attenuated targeting moiety that is directed against a promiscuous antigen or receptor, which may improve targeting to a cell of interest (e.g. via the other targeting moiety) and prevent effects across multiple types of cells, including those not being targeted for therapy (e.g. by binding promiscuous antigen or receptor at a higher affinity than what is provided in these embodiments).
  • the multispecific CD8 binding agent of the invention may be constructed using methods known in the art, see for example, U.S. Patent No. 9,067,991 , U.S. Patent Publication No. 20110262348 and WO 2004/041862, the entire contents of which are hereby incorporated by reference.
  • the multispecific CD8 binding agent of the invention comprising two or more targeting moieties may be constructed by chemical crosslinking, for example, by reacting amino acid residues with an organic derivatizing agent as described by Blattler ef a/., Biochemistry 24,1517-1524 and EP294703, the entire contents of which are hereby incorporated by reference.
  • the multispecific CD8 binding agent comprising two or more targeting moieties is constructed by genetic fusion, i.e., constructing a single polypeptide which includes the polypeptides of the individual targeting moieties.
  • a single polypeptide construct may be formed which encodes a first VHH with an antigen recognition domain against CD8 and a second antibody or antibody derivative with an antigen recognition domain against a tumor antigen.
  • a method for producing bivalent or multivalent VHH polypeptide constructs is disclosed in PCT patent application WO 96/34103, the entire contents of which is hereby incorporated by reference.
  • the multispecific CD8 binding agent of the invention may be constructed by using linkers.
  • the carboxy-terminus of a first VHH with an antigen recognition domain against CD8 may be linked to the amino-terminus of a second antibody or antibody derivative with an antigen recognition domain against a tumor antigen (or vice versa).
  • Exemplary linkers that may be used are described herein.
  • the components of the multispecific CD8 binding agent of the invention are directly linked to each other without the use of linkers.
  • the multi-specific CD8 binding agent of the invention recognizes and binds to CD8 and one or more antigens found on one or more immune cells, which can include, without limitation, megakaryocytes, thrombocytes, erythrocytes, mast cells, basophils, neutrophils, eosinophils, monocytes, macrophages, natural killer cells, T lymphocytes (e.g., cytotoxic T lymphocytes, T helper cells, natural killer T cells), B lymphocytes, plasma cells, dendritic cells, or subsets thereof.
  • the CD8 binding agent specifically binds to an antigen of interest and effectively directly or indirectly recruits one of more immune cells.
  • the multi-specific CD8 binding agent of the invention recognizes and binds to CD8 and one or more antigens found on tumor cells.
  • the present CD8 binding agents may directly or indirectly recruit an immune cell to a tumor cell or the tumor microenvironment.
  • the present CD8 binding agents may directly or indirectly recruit an immune cell, e.g. an immune cell that can kill and/or suppress a tumor cell (e.g., a CTL), to a site of action (such as, by way of non-limiting example, the tumor microenvironment).
  • the present CD8 binding agents are capable of, or find use in methods involving, shifting the balance of immune cells in favor of immune attack of a tumor.
  • the present CD8 binding agents can shift the ratio of immune cells at a site of clinical importance in favor of cells that can kill and/or suppress a tumor (e.g. T cells, cytotoxic T lymphocytes, T helper cells, natural killer (NK) cells, natural killer T (NKT) cells, anti-tumor macrophages (e.g. M1 macrophages), neutrophils, B cells, dendritic cells or subsets thereof and in opposition to cells that protect tumors (e.g.
  • T cells cytotoxic T lymphocytes, T helper cells, natural killer (NK) cells, natural killer T (NKT) cells, anti-tumor macrophages (e.g. M1 macrophages), neutrophils, B cells, dendritic cells or subsets thereof and in opposition to cells that protect tumors (e.g.
  • the present CD8 binding agent is capable of increasing a ratio of effector T cells to regulatory T cells.
  • the multi-specific CD8 binding agent of the invention comprises a targeting moiety having an antigen recognition domain that specifically binds to an antigen associated with tumor cells.
  • the targeting moiety directly or indirectly recruits tumor cells.
  • the recruitment of the tumor cell is to one or more effector cell (e.g. an immune cell as described herein) that can kill and/or suppress the tumor cell.
  • the targeting moiety directly or indirectly recruits T cells to a tumor cell, for example, by virtue of the two targeting moieties interacting with their respective antigens on a tumor and CD8-positive immune cell (e.g. T cell).
  • Tumor cells refer to an uncontrolled growth of cells or tissues and/or an abnormal increased in cell survival and/or inhibition of apoptosis which interferes with the normal functioning of bodily organs and systems.
  • tumor cells include benign and malignant cancers, polyps, hyperplasia, as well as dormant tumors or micro metastases.
  • Illustrative tumor cells include, but are not limited to cells of: basal cell carcinoma, biliary tract cancer; bladder cancer; bone cancer; brain and central nervous system cancer; breast cancer; cancer of the peritoneum; cervical cancer; choriocarcinoma; colon and rectum cancer; connective tissue cancer; cancer of the digestive system; endometrial cancer; esophageal cancer; eye cancer; cancer of the head and neck; gastric cancer (including gastrointestinal cancer); glioblastoma; hepatic carcinoma; hepatoma; intra- epithelial neoplasm; kidney or renal cancer; larynx cancer; leukemia; liver cancer; lung cancer (e.g., small-cell lung cancer, non-small cell lung cancer, adenocarcinoma of the lung, and squamous carcinoma of the lung); melanoma; myeloma; neuroblastoma; oral cavity cancer (lip, tongue, mouth, and pharynx); ovarian cancer; pancreatic cancer
  • Tumor cells, or cancer cells also include, but are not limited to, carcinomas, e.g. various subtypes, including, for example, adenocarcinoma, basal cell carcinoma, squamous cell carcinoma, and transitional cell carcinoma), sarcomas (including, for example, bone and soft tissue), leukemias (including, for example, acute myeloid, acute lymphoblastic, chronic myeloid, chronic lymphocytic, and hairy cell), lymphomas and myelomas (including, for example, Hodgkin and non-Hodgkin lymphomas, light chain, non-secretory, MGUS, and plasmacytomas), and central nervous system cancers (including, for example, brain (e.g. gliomas (e.g.
  • astrocytoma oligodendroglioma, and ependymoma
  • meningioma meningioma
  • pituitary adenoma a neurotrophic factor
  • neuromas a neurotrophic factor-derived neurotrophic factor-derived neurotrophic factor-derived neurotrophic factor-derived neurotrophic factor-derived neurotrophic factor-derived neurotrophic factor-derived neurotrophic factor-derived neurotrophic tumors (e.g. meningiomas and neurofibroma).
  • Illustrative tumor antigens include, but are not limited to, MART-1/Melan-A, gp100, Dipeptidyl peptidase IV (DPPIV), adenosine deaminase-binding protein (ADAbp), cyclophilin b, Colorectal associated antigen (CRC)- 0017-1A/GA733, Carcinoembryonic Antigen (CEA) and its immunogenic epitopes CAP-1 and CAP-2, etv6, aml1 , Prostate Specific Antigen (PSA) and its immunogenic epitopes PSA-1 , PSA-2, and PSA-3, prostate-specific membrane antigen (PSMA), T-cell receptor/CD3-zeta chain, MAGE-family of tumor antigens (e.g., MAGE-A1 , MAGE-A2, MAGE-A3, MAGE-A4, MAGE-A5, MAGE-A6, MAGE-A7, MAGE-A8, MAGE-A9, MAGE-
  • the present multi-specific CD8 binding agent recognizes and binds to CD8 as well as an antigen on a tumor cell. In some embodiments, the multi-specific CD8 binding agent directly or indirectly recruits CTLs to the tumor cell or tumor microenvironment.
  • the present multi-specific CD8 binding agent has targeting moieties which target two different cells (e.g. to make a synapse) or the same cell (e.g. to get a more concentrated signaling agent effect).
  • the multi-specific CD8 binding agent of the invention comprises a targeting moiety having an antigen recognition domain that specifically binds to a target (e.g. antigen, receptor) associated with T cells.
  • a target e.g. antigen, receptor
  • the targeting moiety recruits directly or indirectly T cells.
  • the antigen recognition domains specifically bind to effector T cells.
  • the antigen recognition domain directly or indirectly recruits effector T cells, e.g., in some embodiments, to a therapeutic site (e.g. a locus with one or more disease cell or cell to be modulated for a therapeutic effect).
  • Illustrative effector T cells include cytotoxic T cells (e.g.
  • CD4 + effector T cells e.g. ⁇ TCR, CD3 + , CD4 + , CCR7 + , CD62Lhi, IL7R/CD127 +
  • CD8 + effector T cells e.g. ⁇ TCR, CD3 + , CD8 + , CCR7 + , CD62Lhi, IL7R/CD127 +
  • effector memory T cells e.g. CD62Llow, CD44 + , TCR, CD3 + , IL7R/CD127 + , IL-15R + , CCR7low
  • central memory T cells e.g.
  • CD62L + effector T cells CD62L + effector T cells
  • CD8 + effector memory T cells TEM including early effector memory T cells (CD27 + CD62L " ) and late effector memory T cells (CD27 " CD62L “ ) (TemE and TemL, respectively); CD127( + )CD25(low/-) effector T cells; CD127(-)CD25(-) effector T cells; CD8 + stem cell memory effector cells (TSCM) (e.g.
  • TH1 effector T-cells e.g. CXCR3 + , CXCR6 + and CCR5 + ; ⁇ ⁇ TCR, CD3 + , CD4 + , IL-12R + , IFNyR + , CXCR3 + ), TH2 effector T cells (e.g. CCR3 + , CCR4 + and CCR8 + ; ⁇ ⁇ TCR, CD3 + , CD4 + , IL-4R + , IL-33R + , CCR4 + , IL-17RB + , CRTH2 + ); TH9 effector T cells (e.g.
  • TH17 effector T cells e.g. ⁇ TCR, CD3 + , CD4 + , IL-23R + , CCR6 + , IL-1 R + ); CD4 + CD45RO + CCR7 + effector T cells, ICOS + effector T cells; CD4 + CD45RO + CCR7(-) effector T cells; and effector T cells secreting IL-2, IL-4 and/or IFN- ⁇ .
  • Illustrative T cell antigens of interest include, for example (and inclusive of the extracellular domains, where applicable): CD8, CD3, SLAMF4, IL-2Ra, 4-1 BB/TNFRSF9, IL-2 R ⁇ , ALCAM, B7-1 , IL-4 R, B7-H3, BLAME/SLAMFS, CEACAM1 , IL-6 R, CCR3, IL-7 Ra, CCR4, CXCRI/IL-S RA, CCR5, CCR6, IL-10R a, CCR 7, IL-I 0 R ⁇ , CCRS, IL-12 R ⁇ 1 , CCR9, IL-12 R ⁇ 2, CD2, IL-13 R a 1 , IL-13, CD3, CD4, ILT2/CDS5j, ILT3/CDS5k, ILT4/CDS5d, ILT5/CDS5a, lutegrin a 4/CD49d, CDS, Integrin a E/CD103, CD6, Integrin a M/CD
  • the multi-specific CD8 binding agent of the invention comprises a targeting moiety having an antigen recognition domain that specifically binds to a target (e.g. antigen, receptor) associated with B cells.
  • a target e.g. antigen, receptor
  • the targeting moiety directly or indirectly recruits B cells, e.g., in some embodiments, to a therapeutic site (e.g. a locus with one or more disease cell or cell to be modulated for a therapeutic effect).
  • Illustrative B cell antigens of interest include, for example, CD10, CD19, CD20, CD21 , CD22, CD23, CD24, CD37, CD38, CD39, CD40, CD70, CD72, CD73, CD74, CDw75, CDw76, CD77, CD78, CD79a/b, CD80, CD81 , CD82, CD83, CD84, CD85, CD86, CD89, CD98, CD126, CD127, CDw130, CD138, CDw150, and B-cell maturation antigen (BCMA).
  • the CD8 binding agent comprises a targeting moiety that binds one or more of these illustrative B cell antigens.
  • the multi-specific CD8 binding agent of the invention comprises a targeting moiety having an antigen recognition domain that specifically bind to a target (e.g. antigen, receptor) associated with Natural Killer cells.
  • a target e.g. antigen, receptor
  • the targeting moiety directly or indirectly recruits Natural Killer cells, e.g., in some embodiments, to a therapeutic site (e.g. a locus with one or more disease cell or cell to be modulated for a therapeutic effect).
  • Illustrative Natural Killer cell antigens of interest include, for example TIGIT, 2B4/SLAMF4, KIR2DS4, CD155/PVR, KIR3DL1 , CD94, LMIR1/CD300A, CD69, LMIR2/CD300c, CRACC/SLAMF7, LMIR3/CD300LF, Kirlalpha, DNAM-1 , LMIR5/CD300LB, Fc-epsilon Rll, LMIR6/CD300LE, Fc- ⁇ RI/CD64, MICA, Fc-Y RIIB/CD32b, MICB, Fc- ⁇ RIIC/CD32c, MULT-1 , Fc- ⁇ RIIA/CD32a, Nectin-2/CD112, Fc- ⁇ RIII/CD16, NKG2A, FcRH1/IRTA5, NKG2C, FcRH2/IRTA4, NKG2D, FcRH4/IRTA1 , NKp30, Fc
  • the multi-specific CD8 binding agent of the invention comprises a targeting moiety having an antigen recognition domain that specifically binds to a target (e.g. antigen, receptor) associated with macrophages/monocytes.
  • a target e.g. antigen, receptor
  • the targeting moiety directly or indirectly recruits macrophages/monocytes, e.g., in some embodiments, to a therapeutic site (e.g. a locus with one or more disease cell or cell to be modulated for a therapeutic effect).
  • Illustrative macrophages/monocyte antigens of interest include, for example SIRPIa, B7-1/CD80, ILT4/CD85d, B7-H1 , ILT5/CD85a, Common ⁇ Chain, Integrin a 4/CD49d, BLAME/SLAMF8, Integrin a X/CDIIc, CCL6/C10, Integrin ⁇ 2/CD18, CD155/PVR, Integrin ⁇ 3/CD61 , CD31/PECAM-1 , Latexin, CD36/SR-B3, Leukotriene B4 R1 , CD40/TNFRSF5, LIMPIIISR-B2, CD43, LMIR1/CD300A, CD45, LMIR2/CD300c, CD68, LMIR3/CD300LF, CD84/SLAMF5, LMIR5/CD300LB, CD97, LMIR6/CD300LE, CD163, LRP-1 , CD2F-10/SLAMF9, MARCO, CRACC/S
  • the multi-specific CD8 binding agent of the invention comprises a targeting moiety having an antigen recognition domain that specifically binds to a target (e.g. antigen, receptor) associated with dendritic cells.
  • a target e.g. antigen, receptor
  • the targeting moiety directly or indirectly recruits dendritic cells, e.g., in some embodiments, to a therapeutic site (e.g. a locus with one or more disease cell or cell to be modulated for a therapeutic effect).
  • Illustrative dendritic cell antigens of interest include, for example, CLEC9A, XCR1 , RANK, CD36/SRB3, LOX-1/SR-E1 , CD68, MARCO, CD163, SR-A1/MSR, CD5L, SREC-1 , CL-PI/COLEC12, SREC-II, LIMPIIISRB2, RP105, TLR4, TLR1 , TLR5, TLR2, TLR6, TLR3, TLR9, 4-IBB Ligand/TNFSF9, IL-12/IL-23 p40, 4- Amino-1 ,8-naphthalimide, ILT2/CD85j, CCL21/6Ckine, ILT3/CD85k, 8-oxo-dG, ILT4/CD85d, 8D6A, ILT5/CD85a, A2B5, lutegrin a 4/CD49d, Aag, Integrin ⁇ 2/CD18, AMICA, Langerin, B7-2/CD
  • the multi-specific CD8 binding agent of the invention comprises a targeting moiety having an antigen recognition domain that specifically binds a target (e.g. antigen, receptor) on immune cells selected from, but not limited to, megakaryocytes, thrombocytes, erythrocytes, mast cells, basophils, neutrophils, and eosinophils.
  • a target e.g. antigen, receptor
  • the antigen recognition domains directly or indirectly recruit megakaryocytes, thrombocytes, erythrocytes, mast cells, basophils, neutrophils, and eosinophil, e.g., in some embodiments, to a therapeutic site (e.g. a locus with one or more disease cell or cell to be modulated for a therapeutic effect).
  • the multi-specific CD8 binding agent of the invention comprises a targeting moiety having an antigen recognition domain that specifically binds to a target (e.g. antigen, receptor) associated with megakaryocytes and/or thrombocytes.
  • a target e.g. antigen, receptor
  • Illustrative megakaryocyte and/or thrombocyte antigens of interest include, for example, GP llb/llla, GPIb, vWF, PF4, and TSP.
  • the CD8 binding agent comprises a targeting moiety that binds one or more of these illustrative megakaryocyte and/or thrombocyte antigens.
  • the multi-specific CD8 binding agent of the invention comprises a targeting moiety having an antigen recognition domain that specifically binds to a target (e.g. antigen, receptor) associated with erythrocytes.
  • a target e.g. antigen, receptor
  • Illustrative erythrocyte antigens of interest include, for example, CD34, CD36, CD38, CD41a (platelet glycoprotein llb/llla), CD41 b (GPIIb), CD71 (transferrin receptor), CD105, glycophorin A, glycophorin C, c-kit, HLA-DR, H2 (MHC-II), and Rhesus antigens.
  • the CD8 binding agent comprises a targeting moiety that binds one or more of these illustrative erythrocyte antigens.
  • the multi-specific CD8 binding agent of the invention comprises a targeting moiety having an antigen recognition domain that specifically binds to a target (e.g. antigen, receptor) associated with mast cells.
  • a target e.g. antigen, receptor
  • Illustrative mast cells antigens of interest include, for example, SCFR/CD117, Fc e RI, CD2, CD25, CD35, CD88, CD203c, C5R1 , CMAI, FCERIA, FCER2, TPSABI.
  • the CD8 binding agent comprises a targeting moiety that binds one or more of these mast cell antigens.
  • the multi-specific CD8 binding agent of the invention comprises a targeting moiety having an antigen recognition domain that specifically binds to a target (e.g. antigen, receptor) associated with basophils.
  • a target e.g. antigen, receptor
  • basophils antigens of interest include, for example, Fc e RI, CD203c, CD123, CD13, CD107a, CD107b, and CD164.
  • the CD8 binding agent comprises a targeting moiety that binds one or more of these basophil antigens.
  • the multi-specific CD8 binding agent of the invention comprises a targeting moiety having an antigen recognition domain that specifically binds to a target (e.g. antigen, receptor) associated with neutrophils.
  • a target e.g. antigen, receptor
  • Illustrative neutrophils antigens of interest include, for example, 7D5, CD10/CALLA, CD13, CD16 (FcRIII), CD18 proteins (LFA-1 , CR3, and p150, 95), CD45, CD67, and CD177.
  • the CD8 binding agent comprises a targeting moiety that binds one or more of these neutrophil antigens.
  • the multi-specific CD8 binding agent of the invention comprises a targeting moiety having an antigen recognition domain that specifically binds to a target (e.g.
  • eosinophils antigens of interest include, for example, CD35, CD44 and CD69.
  • the CD8 binding agent comprises a targeting moiety that binds one or more of these eosinophil antigens.
  • the multi-specific CD8 binding agent of the invention comprises a targeting moiety having an antigen recognition domain that specifically binds to any appropriate antigen or receptor or cell surface markers known by the skilled artisan.
  • the antigen or cell surface marker is a tissue-specific marker.
  • tissue-specific markers include, but are not limited to, endothelial cell surface markers such as ACE, CD14, CD34, CDH5, ENG, ICAM2, MCAM, NOS3, PECAMI, PROCR, SELE, SELP, TEK, THBD, VCAMI, VWF; smooth muscle cell surface markers such as ACTA2, MYHIO, MYH1 1 , MYH9, MYOCD; fibroblast (stromal) cell surface markers such as ALCAM, CD34, COLIAI, COL1A2, COL3A1 , FAP, PH-4; epithelial cell surface markers such as CDID, K6IRS2, KRTIO, KRT13, KRT17, KRT18, KRT19, KRT4, KRT5, KRT8, MUCI, TACSTDI; neovasculature markers such as CD13, TFNA, Alpha-v beta-3 ( ⁇ 3), E-selectin; and adipocyte surface markers such as ADIPOQ, FA
  • the multi-specific CD8 binding agent of the invention comprises a targeting moiety having an antigen recognition domain that specifically binds to a checkpoint marker expressed on a T cell, e.g. one or more of PD-1 , CD28, CTLA4, ICOS, BTLA, KIR, LAG3, CD137, OX40, CD27, CD40L, TIM3, and A2aR.
  • the multi-specific CD8 binding agent of the invention comprises a targeting moiety having an antigen recognition domain that specifically binds to a checkpoint marker, e.g.
  • PD- 1/PD-L1 or PD-L2 CD28/CD80 or CD86
  • CTLA4/ CD80 or CD86 CTLA4/ CD80 or CD86
  • ICOS/ICOSL or B7RP1 BTLA/HVEM
  • KIR KIR
  • LAG3, CD137/CD137L, OX40/OX40L, CD27, CD40L, TIM3/Gal9, and A2aR KIR
  • LAG3, CD137/CD137L CD137/CD137L
  • OX40/OX40L CD27, CD40L, TIM3/Gal9
  • A2aR A2aR.
  • the present multispecific CD8 binding agent comprises a targeting moiety directed against (i) CD8; (ii) a checkpoint marker expressed on a T cell, e.g. one or more of PD-1 , CD28, CTLA4, ICOS, BTLA, KIR, LAG3, CD137, OX40, Cd27, CD40L, TIM3, and A2aR and/or (iii) a targeting moiety is directed against a tumor cell, along with any of the modified (e.g. mutant) signaling agents described herein.
  • a targeting moiety directed against (i) CD8; (ii) a checkpoint marker expressed on a T cell, e.g. one or more of PD-1 , CD28, CTLA4, ICOS, BTLA, KIR, LAG3, CD137, OX40, Cd27, CD40L, TIM3, and A2aR and/or (iii) a targeting moiety is directed against a tumor cell, along with any of the modified (e.
  • the present multi-specific CD8 binding agent has one or more targeting moieties directed against PD-1.
  • the CD8 binding agent has one or more targeting moieties which selectively bind a PD-1 polypeptide.
  • the CD8 binding agent comprises one or more antibodies, antibody derivatives or formats, peptides or polypeptides, or fusion proteins that selectively bind a PD-1 polypeptide.
  • the targeting moiety comprises the anti-PD-1 antibody pembrolizumab (aka MK-3475, KEYTRUDA), or fragments thereof.
  • pembrolizumab and other humanized anti-PD-1 antibodies are disclosed in Hamid, et al. (2013) New England Journal of Medicine 369 (2): 134-44, US 8,354,509, and WO 2009/114335, the entire disclosures of which are hereby incorporated by reference.
  • pembrolizumab or an antigen-binding fragment thereof for use in the methods provided herein comprises a heavy chain comprising the amino acid sequence of:
  • the targeting moiety comprises the anti-PD-1 antibody, nivolumab (aka BMS-936558, MDX- 1106, ONO-4538, OPDIVO), or fragments thereof.
  • nivolumab (clone 5C4) and other human monoclonal antibodies that specifically bind to PD-1 are disclosed in US 8,008,449 and WO 2006/121168, the entire disclosures of which are hereby incorporated by reference.
  • nivolumab or an antigen- binding fragment thereof comprises a heavy chain comprising the amino acid sequence of:
  • the targeting moiety comprises the anti-PD-1 antibody pidilizumab (aka CT-011 , hBAT or hBAT-1), or fragments thereof.
  • pidilizumab and other humanized anti-PD-l monoclonal antibodies are disclosed in US 2008/0025980 and WO 2009/101611 , the entire disclosures of which are hereby incorporated by reference.
  • the anti-PD-1 antibody or an antigen-binding fragment thereof for use in the methods provided herein comprises a light chain variable regions comprising an amino acid sequence selected from SEQ ID NOS: 15-18 of US 2008/0025980:
  • SEQ ID No: 23 of US 2008/0025980 (SEQ ID NO:48): QIQLVQSGSELKKPGASVKISCKASGYTFTNYGMNWVRQAPGQGLQWMGWINTDSGESTY AEEFKGRFVFSLDTSVNTAYLQITSLTAEDTGMYFCVRVGYDALDYWGQGTLVTVSS;
  • the targeting moiety comprises a light chain comprising SEQ ID N0:18 of US 2008/0025980 and a heavy chain comprising SEQ ID NO:22 of US 2008/0025980.
  • the targeting moiety comprises AMP-514 (aka MEDI-0680).
  • the targeting moiety comprises the PD-L2-Fc fusion protein AMP-224, which is disclosed in WO2010/027827 and WO 2011/066342, the entire disclosures of which are hereby incorporated by reference.
  • the targeting moiety may include a targeting domain which comprises SEQ ID NO:4 of WO2010/027827 (SEQ ID NO:50): LFTVTVPKELYI IEHGSNVTLECNFDTGSHVNLGAI ASLQKVEND SPHRERATLLEEQ LPLGKASFHIPQVQVRDEGQYQCI I IYGVAWDYKYLTLKVKASYRKINTHILKVPETDEV ELTCQATGYPLAEVSWPNVSVPANTSHSRTPEGLYQVTSVLRLKPPPGRNFSCVFWNTHV RELTLASIDLQSQMEPRTHPTWLLHIFIPFCI IAFIFIATVIALRKQLCQKLYSSKDTTK RPVTTTKREVNSAI
  • B7-DC fusion protein which comprises SEQ ID NO:83 of WO2010/027827 (SEQ ID NO:51):
  • MIFLLLMLSLELQLHQIAALFTVTVPKELYI IEHGSNVTLECNFDTGSHVNLGAI ASLQ KVENDTSPHRERATLLEEQLPLGKASFHIPQVQVRDEGQYQCI I IYGVAWDYKYLTLKVK ASYRKINTHILKVPETDEVELTCQATGYPLAEVSWPNVSVPANTSHSRTPEGLYQVTSVL RLKPPPGRNFSCVFWNTHVRELTLASIDLQSQMEPRTHPTWEPKSCDKTHTCPPCPAPEL LGGPSVFLFPPKPKDTLMISRTPEVTCVWDVSHEDPEVKFNWYVDGVEVHNAKTKPREE QYNSTYRWSVLTVLHQDWLNGKEYKCKVSNKALPAPIEKTISKAKGQPREPQVYTLPPS RDELTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSKLTVDK SRWQQGNVFSCS
  • the targeting moiety comprises the peptide AUNP 12 or any of the other peptides disclosed in US 2011/0318373 or 8,907,053.
  • the targeting moiety may comprise AUNP 12 ⁇ i.e., Compound 8 or SEQ ID NO:49 of US 2011/0318373) which has the sequence of SEQ ID NO:52:
  • SNTSESFK (SNTSESF) FRVTQLAPKAQIKE-NH2
  • the targeting moiety comprises the anti-PD-1 antibody 1 E3, or fragments thereof, as disclosed in US 2014/0044738, the entire disclosures of which are hereby incorporated by reference.
  • 1 E3 or an antigen-binding fragment thereof for use in the methods provided herein comprises a heavy chain variable region comprising the amino acid sequence of: EVQLQQSGPV LVKPGASVKM SCKASGYTFT DYYMNWVKQS HGKSLEWIGN INPYNGGTTY NQKFKGKATL TVDKSSRTAY MEINSLTSED SAVYYCARGR IYDGSLDYWG QGTALTVSS (SEQ ID NO:53) ;
  • a light chain variable region comprising the amino acid sequence of: DIQMTQFPSS LCASQGGKVT VTCKASQDIN NYMAWYQHKP GKGPRLLIHY TSTLLSGIPS RFSGSGSGRD YSFSISNLEP EDIATYYCLQ YDNLWTFGGG
  • TKLEiK (SEQ ID NO:54) .
  • the targeting moiety comprises the anti-PD-1 antibody 1 E8, or fragments thereof, as disclosed in US 2014/0044738, the entire disclosures of which are hereby incorporated by reference.
  • 1 E8 or an antigen-binding fragment thereof for use in the methods provided herein comprises a heavy chain variable region comprising the amino acid sequence of:
  • the targeting moiety comprises the anti-PD-1 antibody 1 H3, or fragments thereof, as disclosed in US 2014/0044738, the entire disclosures of which are hereby incorporated by reference.
  • 1 H3 or an antigen-binding fragment thereof for use in the methods provided herein comprises a heavy chain variable region comprising the amino acid sequence of:
  • the targeting moiety comprises a VHH directed against PD-1 as disclosed, for example, in US 8,907,065 and WO 2008/071447, the entire disclosures of which are hereby incorporated by reference.
  • the VHHs against PD-1 comprise SEQ ID NOS: 347-351 of US 8,907,065:
  • SEQ ID No: 347 of US 8,907,065 (SEQ ID NO:59): EVQLVESGGGLVQAGKSLRLSCAASGSI FSIHAMGWFRQAPGKEREFVAA ITWSGGITYYEDSVKGRFTI SRDNAKNTVYLQMNSLKPEDTAIYYCAADR AESSWYDYWGQGTQVTVSS ;
  • SEQ ID No: 348 of US 8,907,065 (SEQ ID NO:60): EVQLVESGGGLVQAGGSLRLSCAASGSIASIHAMGWFRQAPGKEREFVAV ITWSGGITYYADSVKGRFTI SRDNAKNTVYLQMNSLKPEDTAIYYCAGDK HQSSWYDYWGQGTQVTVSS ;
  • SEQ ID No: 349 of US 8,907,065 (SEQ ID NO:61): EVQLVESGGGLVQAGGSLRLSCAASGSISSIHAMGWFRQAPGKEREFVAA ITWSGGITYYADSLKGRFTI SRDNAKNTGYLQMNSLKPEDTAIYYCAADR AQSSWYDYWGQGTQVTVSS ;
  • SEQ ID No: 350 of US 8,907,065 (SEQ ID NO:62): EVQLVESGGGLVQAGGSLGLSCAASGSI FSINAMAWFRQAPGKEREFVAL I SWSGGSTYYEDSVKGRFTI SRDNAKNTVYLQMNSLKPEDTAIYYCAADR VDSNWYDYWGQGTQVTVSS ;
  • SEQ ID No: 351 of US 8,907,065 (SEQ ID NO:63): EVQLVESGGGLVQAGGSLRLSCAASGRAFSSGTMGWFRRAPGKEREFVA SIPWSGGRIYYADSVKGRFTISRDNAQNTVYLQMNSLKPEDTAVYYCAVK ERSTGWDFASWGQCTQVTVSS .
  • the targeting moiety comprises any one of the anti-PD-1 antibodies, or fragments thereof, as disclosed in US2011/0271358 and WO2010/036959, the entire contents of which are hereby incorporated by reference.
  • the antibody or an antigen-binding fragment thereof for use in the methods provided herein comprises a heavy chain comprising an amino acid sequence selected from SEQ ID NOS: 25-29 of US2011/0271358:
  • SEQ ID No: 25 of US2011/0271358 (SEQ ID NO:64): QVQLVQSGAELKQPGASVKMSCKASGYSFTSSWIHWVKQAPGQGLEWIGYIYPSTGFTEY NQKFKDRATLTADKSTSTAYMELSSLRSEDSAVYYCARWRDSSGYHAMDYWGQGTSVTVS
  • SEQ ID No: 26 of US2011/0271358 (SEQ ID NO:65): QVQLVQSGAEVKQPGASVKMSCKASGYSFTSSWIHWVKQAPGQGLEWIGYIYPSTGFTEY
  • a light chain comprising an amino acid sequence selected from SEQ ID NOS: 30-33 of US2011/0271358: SEQ ID No: 30 of US2011/0271358 (SEQ ID NO:69):
  • SEQ ID No: 32 of US2011/0271358 (SEQ ID NO:71): EIVLTQSPATLSLSPGQRLTISCRASQSVSTSGYSYMHWYQQKPDQSPKLLIKFGSNLES GIPARFSGSGSGTDFTLTISSLEPEDFATYYCQHSWEIPYTFGQGTKLEIK;
  • the present multi-specific CD8 binding agent comprises one or more antibodies directed against PD-1 , or antibody fragments thereof, selected from TSR-042 (Tesaro, Inc.), REGN2810 (Regeneron Pharmaceuticals, Inc.), PDR001 (Novartis Pharmaceuticals), and BGB-A317 (BeiGene Ltd.)
  • the present multi-specific CD8 binding agent has one or more targeting moieties directed against PD-L1.
  • the CD8 binding agent has one or more targeting moieties which selectively bind a PD-L1 polypeptide.
  • the CD8 binding agent comprises one or more antibodies, antibody derivatives or formats, peptides or polypeptides, or fusion proteins that selectively bind a PD-L1 polypeptide.
  • the targeting moiety comprises the anti-PD-L1 antibody MEDI4736 (aka durvalumab), or fragments thereof.
  • MEDI4736 is selective for PD-L1 and blocks the binding of PD-L1 to the PD-1 and CD80 receptors.
  • MEDI4736 and antigen-binding fragments thereof for use in the methods provided herein comprises a heavy chain and a light chain or a heavy chain variable region and a light chain variable region.
  • the sequence of MEDI4736 is disclosed in WO/2017/06272, the entire contents of which are hereby incorporated by reference.
  • MEDI4736 or an antigen-binding fragment thereof for use in the methods provided herein comprises a heavy chain comprising the amino acid sequence of:
  • VLDSDGSFFL YSKLTVDKSR WQQGNVFSCS VMHEALHNHY TQKSLSLSPG
  • EIVLTQSPGT LSLSPGERAT LSCRASQRVS SSYLAWYQQK PGQAPRLLIY DASSRATGIP DRFSGSGSGT DFTLTISRLE PEDFAVYYCQ QYGSLPWTFG QGTKVEIKRT VAAPSVFIFP PSDEQLKSGT ASVVCLLNNF YPREAKVQWK VDNALQSGNS QESVTEQDSK DSTYSLSSTL TLSKADYEKH KVYACEVTHQ GLSSPVTKSF NRGEC (SEQ ID N074) .
  • the MEDI4736 or an antigen-binding fragment thereof for use in the methods provided herein comprises a heavy chain variable region comprising the amino acid sequence of SEQ ID NO:4 of WO/2017/06272 (SEQ ID NO:75):
  • the targeting moiety comprises the anti-PD-L1 antibody atezolizumab (aka MPDL3280A, RG7446), or fragments thereof.
  • atezolizumab or an antigen-binding fragment thereof for use in the methods provided herein comprises a heavy chain comprising the amino acid sequence of:
  • the targeting moiety comprises the anti-PD-L1 antibody avelumab (aka MSB0010718C), or fragments thereof.
  • avelumab or an antigen-binding fragment thereof for use in the methods provided herein comprises a heavy chain comprising the amino acid sequence of: EVQLLESGGG LVQPGGSLRL SCAASGFTFS SYIMMWVRQA PGKGLEWVSS
  • LDSDGSFFLY SKLTVDKSRW QQGNVFSCSV MHEALHNHYT QKSLSLSPGK (SEQ ID NO:79) ; and/or a light chain comprising the amino acid sequence of:
  • the targeting moiety comprises the anti-PD-L1 antibody BMS-936559 (aka 12A4, MDX-1105), or fragments thereof, as disclosed in US 2013/0309250 and WO2007/005874, the entire disclosures of which are hereby incorporated by reference.
  • BMS-936559 or an antigen-binding fragment thereof for use in the methods provided herein comprises a heavy chain variable region comprising the amino acid sequence of:
  • VSS (SEQ ID NO:81) ;
  • a light chain variable region comprising the amino acid sequence of: EIVLTQSPATLSLSPGERATLSCRASQSVSSYLAWYQQKPGQAPRLLIYDASNRATGIPA RFSGSGSGTDFTLTI SSLEPEDFAVYYCQQRSNWPTFGQGTKVEIK (SEQ ID NO:82) .
  • the targeting moiety comprises the anti-PD-L1 antibody 3G10, or fragments thereof, as disclosed in US 2013/0309250 and WO2007/005874, the entire disclosures of which are hereby incorporated by reference.
  • 3G10 or an antigen-binding fragment thereof for use in the methods provided herein comprises a heavy chain variable region comprising the amino acid sequence of:
  • the targeting moiety comprises the anti-PD-L1 antibody 10A5, or fragments thereof, as disclosed in US 2013/0309250 and WO2007/005874, the entire disclosures of which are hereby incorporated by reference.
  • 10A5 or an antigen-binding fragment thereof for use in the methods provided herein comprises a heavy chain variable region comprising the amino acid sequence of:
  • the targeting moiety comprises the anti-PD-L1 antibody 5F8, or fragments thereof, as disclosed in US 2013/0309250 and WO2007/005874, the entire disclosures of which are hereby incorporated by reference.
  • 5F8 or an antigen-binding fragment thereof for use in the methods provided herein comprises a heavy chain variable region comprising the amino acid sequence of:
  • a light chain variable region comprising the amino acid sequence of: EIVLTQSPGTLSLSPGERATLSCRASQSVSSSYLAWYQQKPGQAPRLLIYGASSRATGIP
  • the targeting moiety comprises the anti-PD-L1 antibody 10H10, or fragments thereof, as disclosed in US 2013/0309250 and WO2007/005874, the entire disclosures of which are hereby incorporated by reference.
  • 10H10 or an antigen-binding fragment thereof for use in the methods provided herein comprises a heavy chain variable region comprising the amino acid sequence of:
  • the targeting moiety comprises the anti-PD-L1 antibody 1 B12, or fragments thereof, as disclosed in US 2013/0309250 and WO2007/005874, the entire disclosures of which are hereby incorporated by reference.
  • 1 B12 or an antigen-binding fragment thereof for use in the methods provided herein comprises a heavy chain variable region comprising the amino acid sequence of:
  • VSS (SEQ ID NO:91 ) ;
  • the targeting moiety comprises the anti-PD-L1 antibody 7H1 , or fragments thereof, as disclosed in US 2013/0309250 and WO2007/005874, the entire disclosures of which are hereby incorporated by reference.
  • 7H1 or an antigen-binding fragment thereof for use in the methods provided herein comprises a heavy chain variable region comprising the amino acid sequence of:
  • VSS (SEQ ID NO:93) ;
  • the targeting moiety comprises the anti-PD-L1 antibody 1 1 E6, or fragments thereof, as disclosed in US 2013/0309250 and WO2007/005874, the entire disclosures of which are hereby incorporated by reference.
  • 11 E6 or an antigen-binding fragment thereof for use in the methods provided herein comprises a heavy chain variable region comprising the amino acid sequence of:
  • the targeting moiety comprises the anti-PD-L1 antibody 12B7, or fragments thereof, as disclosed in US 2013/0309250 and WO2007/005874, the entire disclosures of which are hereby incorporated by reference.
  • 12B7 or an antigen-binding fragment thereof for use in the methods provided herein comprises a heavy chain variable region comprising the amino acid sequence of: QVQLVQSGAEVKEPGSSVKVSCKASGGTFNSYAISWVRQAPGQGLEWMGGIIPLFGIAHY AQKFQGRVTITADESTNTAYMDLSSLRSEDTAVYYCARKYSYVSGSPFGMDVWGQGTTVT
  • VSS (SEQ ID NO:97) ;
  • a light chain variable region comprising the amino acid sequence of: EIVLTQSPATLSLSPGERATLSCRASQSVSSYLAWYQQKPGQAPRLLIYDASNRATGIPA RFSGSGTDFTLTISSLEPEDFAVYYCQQRSNWPTFGQGTRLEIK (SEQ ID NO:98) .
  • the targeting moiety comprises the anti-PD-L1 antibody 13G4, or fragments thereof, as disclosed in US 2013/0309250 and WO2007/005874, the entire disclosures of which are hereby incorporated by reference.
  • 13G4 or an antigen-binding fragment thereof for use in the methods provided herein comprises a heavy chain variable region comprising the amino acid sequence of:
  • the targeting moiety comprises the anti-PD-L1 antibody 1 E12, or fragments thereof, as disclosed in US 2014/0044738, the entire disclosures of which are hereby incorporated by reference.
  • 1 E12 or an antigen-binding fragment thereof for use in the methods provided herein comprises a heavy chain variable region comprising the amino acid sequence of:
  • the targeting moiety comprises the anti-PD-L1 antibody 1 F4, or fragments thereof, as disclosed in US 2014/0044738, the entire disclosures of which are hereby incorporated by reference.
  • 1 F4 or an antigen-binding fragment thereof for use in the methods provided herein comprises a heavy chain variable region comprising the amino acid sequence of:
  • the targeting moiety comprises the anti-PD-L1 antibody 2G1 1 , or fragments thereof, as disclosed in US 2014/0044738, the entire disclosures of which are hereby incorporated by reference.
  • 2G1 1 or an antigen-binding fragment thereof for use in the methods provided herein comprises a heavy chain variable region comprising the amino acid sequence of: EVKLQESGPS LVKPSQTLSL TCSVTGYSII SDYWNWIRKF PGNKLEYLGY ISYTGSTYYN PSLKSRISIT RDTSKNQYYL QLNSVTTEDT ATYYCARRGG WLLPFDYWGQ GTTLTVSS (SEQ ID NO: 105) ;
  • a light chain variable region comprising the amino acid sequence of: DIVMTQSPSS LAVSVGEKVS MGCKSSQSLL YSSNQKNSLA WYQQKPGQSP KLLIDWASTR ESGVPDRFTG SGSGTDFTLT ISSVKAEDLA VYYCQQYYGY PLTFGAGTKL ELK (SEQ ID NO:106) .
  • the targeting moiety comprises the anti-PD-L1 antibody 3B6, or fragments thereof, as disclosed in US 2014/0044738, the entire disclosures of which are hereby incorporated by reference.
  • 3B6 or an antigen-binding fragment thereof for use in the methods provided herein comprises a heavy chain variable region comprising the amino acid sequence of:
  • a light chain variable region comprising the amino acid sequence of: DIVMTQSPAI MSASPGEKVT MTCSASSSIR YMHWYQQKPG TSPKRWISDT
  • TKLEiK (SEQ ID NO:108) .
  • the targeting moiety comprises the anti-PD-L1 antibody 3D10, or fragments thereof, as disclosed in US 2014/0044738 and WO2012/145493, the entire disclosures of which are hereby incorporated by reference.
  • 3D10 or an antigen-binding fragment thereof for use in the methods provided herein comprises a heavy chain variable region comprising the amino acid sequence of:
  • TKLELK (SEQ ID NO:110) .
  • the targeting moiety comprises any one of the anti-PD-L1 antibodies disclosed in US2011/0271358 and WO2010/036959, the entire contents of which are hereby incorporated by reference.
  • the antibody or an antigen-binding fragment thereof for use in the methods provided herein comprises a heavy chain comprising an amino acid sequence selected from SEQ ID Nos: 34-38 of US2011/0271358:
  • SEQ ID No: 38 of US2011/0271358 (SEQ ID NO:115): EVQLVQSGAEVKKPGASVKVSCKASGYTFTSYVMHWVRQAPGQRLEWIGYVNPFNDGTKY NEMFKGRATITSDKSTSTAYMELSSLRSEDTAVYYCARQAWGYPWGQGTLVTVSS; and/or a light chain comprising an amino acid sequence selected from SEQ ID Nos: 39-42 of US2011/0271358: SEQ ID No: 39 of US2011/0271358 (SEQ ID NO:116):
  • the targeting moiety comprises the anti-PD-L1 antibody 2.7A4, or fragments thereof, as disclosed in WO 2011/066389, US8,779,108, and US2014/0356353, the entire disclosures of which are hereby incorporated by reference.
  • 2.7A4 or an antigen-binding fragment thereof for use in the methods provided herein comprises a heavy chain variable region comprising the amino acid sequence of:
  • SEQ ID No: 2 of WO 2011/066389 (SEQ ID NO:120): EVQLVESGGGLVKPGGSLRLSCAASGFTFSTYSMNWVRQAPGKGLEWVSSISSSGDYIYY ADSVKGRFTISRDNAKNSLFLQMNSLKAEDTAVYYCARDLVTSMVAFDYWGQGTLVTVSS;
  • a light chain variable region comprising the amino acid sequence of: SEQ ID No: 7 of WO 2011/066389 (SEQ ID NO:121): SYELTQPPSVSVSPGQAARITCSGDALPQKYVFWYQQKSGQAPVLVIYEDSKRPSGIPER FSGSSSGTMATL ISGAQVEDEADYYCYSTDRSGNHRVFGGGTRLTVL .
  • the targeting moiety comprises the anti-PD-L1 antibody 2.9D10, or fragments thereof, as disclosed in WO 2011/066389, US8,779,108, and US2014/0356353, the entire disclosures of which are hereby incorporated by reference.
  • 2.9D10 or an antigen-binding fragment thereof for use in the methods provided herein comprises a heavy chain variable region comprising the amino acid sequence of:
  • SEQ ID No: 12 of WO 2011/066389 (SEQ ID NO:122): EVQLVESGGGLVQPGGSLRLSCAASGFTFSSYWMSWVRQAPGKGLEWVANIKQDGGEQYY
  • the targeting moiety comprises the anti-PD-L1 antibody 2.14H9, or fragments thereof, as disclosed in WO 201 1/066389, US8.779.108, and US2014/0356353, the entire disclosures of which are hereby incorporated by reference.
  • 2.14H9 or an antigen-binding fragment thereof for use in the methods provided herein comprises a heavy chain variable region comprising the amino acid sequence of:
  • a light chain variable region comprising the amino acid sequence of: SEQ ID No: 27 of WO 201 1/066389 (SEQ ID NO:125): EIVLTQSPGTLSLSPGERATLSCRASQRVSSSYLAWYQQKPGQAPRLLIYDASSRATGIP DRFSGSGSGTDFTLTISRLEPEDFAVYYCQQYGSLPWTFGQGTEVEIK.
  • the targeting moiety comprises the anti-PD-L1 antibody 2.20A8, or fragments thereof, as disclosed in WO 201 1/066389, US8.779.108, and US2014/0356353, the entire disclosures of which are hereby incorporated by reference.
  • 2.20A8 or an antigen-binding fragment thereof for use in the methods provided herein comprises a heavy chain variable region comprising the amino acid sequence of:
  • a light chain variable region comprising the amino acid sequence of: SEQ ID No: 37 of WO 201 1/066389 (SEQ ID NO:127):
  • the targeting moiety comprises the anti-PD-L1 antibody 3.15G8, or fragments thereof, as disclosed in WO 201 1/066389, US8.779.108, and US2014/0356353, the entire disclosures of which are hereby incorporated by reference.
  • 3.15G8 or an antigen-binding fragment thereof for use in the methods provided herein comprises a heavy chain variable region comprising the amino acid sequence of:
  • the targeting moiety comprises the anti-PD-L1 antibody 3.18G1 , or fragments thereof, as disclosed in WO 201 1/066389, US8.779.108, and US2014/0356353, the entire disclosures of which are hereby incorporated by reference.
  • 3.18G1 or an antigen-binding fragment thereof for use in the methods provided herein comprises a heavy chain variable region comprising the amino acid sequence of:
  • a light chain variable region comprising the amino acid sequence of: SEQ ID No: 57 of WO 201 1/066389 (SEQ ID NO:131 ):
  • the targeting moiety comprises the anti-PD-L1 antibody 2.7A40PT, or fragments thereof, as disclosed in WO 201 1/066389, US8.779.108, and US2014/0356353, and US2014/0356353, the entire disclosures of which are hereby incorporated by reference.
  • 2.7A40PT or an antigen- binding fragment thereof for use in the methods provided herein comprises a heavy chain variable region comprising the amino acid sequence of:
  • the targeting moiety comprises the anti-PD-L1 antibody 2.14H90PT, or fragments thereof, as disclosed in WO 2011/066389, US8.779.108, and US2014/0356353, the entire disclosures of which are hereby incorporated by reference.
  • 2.14H90PT or an antigen-binding fragment thereof for use in the methods provided herein comprises a heavy chain variable region comprising the amino acid sequence of:
  • SEQ ID No: 72 of WO 2011/066389 (SEQ ID NO:134): EVQLVESGGGLVQPGGSLRLSCAASGFTFSRYWMSWVRQAPGKGLEWVANIKQDGSEKYY VDSVKGRFTISRDNAKNSLYLQMNSLRAEDTAVYYCAREGGWFGELAFDYWGQGTLVTVS
  • a light chain variable region comprising the amino acid sequence of: SEQ ID No: 77 of WO 2011/066389 (SEQ ID NO:135):
  • the targeting moiety comprises any one of the anti-PD-L1 antibodies disclosed in WO2016/061142, the entire contents of which are hereby incorporated by reference.
  • the antibody or an antigen-binding fragment thereof for use in the methods provided herein comprises a heavy chain comprising an amino acid sequence selected from SEQ ID Nos: 18, 30, 38, 46, 50, 54, 62, 70, and 78 of WO2016/061142:
  • SEQ ID No: 18 of WO2016/061142 (SEQ ID NO:136): QVQLVQSGAEVKKPGASVKVSCKASGYTFTSYWMYWVRQATGQGLEWMGRIDPNSGSTKY NEKFKNRFTISRDDSKNTAYLQMNSLKTEDTAVYYCARDYRKGLYAMDYWGQGTTVTVSS;
  • SEQ ID No: 62 of WO2016/061142 (SEQ ID NO:142): EVQLVQSGAEVKKPGESLRISCKGSGYTFTSYWMYWVRQARGQRLEWIGRIDPNSGSTKY NEKFKNRLTISKDTSKNQVVLTMTNMDPVDTATYYCARDYRKGLYAMDYWGQGTTVTVSS;
  • a light chain comprising an amino acid sequence selected from SEQ ID Nos: 22, 26, 34, 42, 58, 66, 74, 82, and 86 of WO2016/061142:
  • the targeting moiety comprises any one of the anti-PD-L1 antibodies disclosed in WO2016/022630, the entire contents of which are hereby incorporated by reference.
  • the antibody or an antigen-binding fragment thereof for use in the methods provided herein comprises a heavy chain comprising an amino acid sequence selected from SEQ ID Nos: 2, 6, 10, 14, 18, 22, 26, 30, 34, 38, 42, and 46 of WO2016/022630:
  • SEQ ID No: 2 of WO2016/022630 (SEQ ID NO:154): EVKLVESGGGLVKPGGSLKLSCAASGFI FRSYGMSWVRQTPEKRLEWVASISSGGSTYYP DSVKGRFTI SRDNARNILYLQMSSLRSEDTAMYDCARGYDSGFAYWGQGTLVTVSE ;
  • SEQ ID No: 18 of WO2016/022630 (SEQ ID NO:158): EVKLFESGGGLVQPGGSLKLSCVASGFDFSTYWMHWVRQAPGQGLEWIGQINPDSTTINY
  • SEQ ID No: 22 of WO2016/022630 (SEQ ID NO:159): EVQLQESGPSLVKPSQTLSLTCSVTGDSITSGYWNWIRKFPGNKLEYMGYISYSGSTYYN PSLKSRISITRDTSKNQYYLQLNSVTTEDTATYYCARSLLWFSTGFAYWGQGTLVTVSA;
  • SEQ ID No: 42 of WO2016/022630 (SEQ ID NO:164): QITLKESGPTLVKPTQTLTLTCTVSGFSLSTYGVHWIRQPPGKALEWLGVIWRGVTTDYN AAFMSRLTITKDNSKNQWLTMNNMDPVDTATYYCARLGFYAMDYWGQGTLVTVSS;
  • SEQ ID No: 24 of WO2016/022630 (SEQ ID NO:171): QIVLTQSPAIMSASPGEKVTLTCSASSSVSSSYLYWNQQKPGSSPKVWIYNTSNLASGVP ARFSGSGSGTSYSLTISSMEAEDAASYFCHQWRSYPPTLGAGTKLELK;
  • SEQ ID No: 44 of WO2016/022630 (SEQ ID NO:176): DIQMTQSPSSLSASVGDRVTITCKASQSVSNDVAWYQQKPGKAPKLLIYYAANRYTGVPD RFSGSGYGTDFTFTI SSLQPEDIATYFCQQDYTSPYTFGQGTKLEIK;
  • the targeting moiety comprises any one of the anti-PD-L1 antibodies disclosed in WO2015/112900, the entire contents of which are hereby incorporated by reference.
  • the antibody or an antigen-binding fragment thereof for use in the methods provided herein comprises a heavy chain comprising an amino acid sequence selected from SEQ ID Nos: 38, 50, 82, and 86 of WO 2015/112900: SEQ ID No: 38 of WO2015/112900 (SEQ ID NO:178):
  • SEQ ID No: 50 of WO 2015/112900 (SEQ ID NO:179): EVQLVQSGAEVKKPGESLRISCKGSGYTFTTYWMHWIRQSPSRGLEWLGNIYPGTGGSNF DEKFKNRFTISRDNSKNTLYLQMNSLRAEDTAVYYCTRWTTGTGAYWGQGTTVTVSS;
  • SEQ ID No: 42 of WO2015/112900 (SEQ ID NO:182): EIVLTQSPATLSLSPGERATLSCKSSQSLLDSGNQKNFLTWYQQKPGQAPRLLIYWASTR ESGVPSRFSGSGSGTEFTLTISSLQPDDFATYYCQNDYSYPYTFGQGTKVEIK;
  • SEQ ID No: 70 of WO 2015/112900 (SEQ ID NO:188): EIVLTQSPATLSLSPGERATLSCKSSQSLLDSGNQKNFLTWYQQKPGQAPRLLIYWASTR ESGVPSRFSGSGTDFTFTISSLEAEDAATYYCQNDYSYPYTFGQGTKVEIK;
  • the targeting moiety comprises any one of the anti-PD-L1 antibodies disclosed in WO 2010/077634 and US 8,217,149, the entire disclosures of which are hereby incorporated by reference.
  • the anti-PD-L1 antibody or an antigen-binding fragment thereof for use in the methods provided herein comprises a heavy chain region comprising the amino acid sequence of: SEQ ID No: 20 of WO 2010/077634 (SEQ ID NO:191):
  • the targeting moiety comprises any one of the anti-PD-L1 antibodies obtainable from the hybridoma accessible under CNCM deposit numbers CNCM 1-4122, CNCM I-4080 and CNCM 1-4081 as disclosed in US 20120039906, the entire disclosures of which are hereby incorporated by reference.
  • the targeting moiety comprises a VHH directed against PD-L1 as disclosed, for example, in US 8,907,065 and WO 2008/071447, the entire disclosures of which are hereby incorporated by reference.
  • the VHHs against PD-L1 comprise SEQ ID NOS: 394-399 of US 8,907,065:
  • SEQ ID No: 394 of US 8,907,065 (SEQ ID NO:193): EVQLVESGGGLVQPGGSLRLSCAASGFTLDYYAIGWFRQAPGKEREWASS I SSSDGSTYYADSVKGRFTI SRDNAKNTVFLQMNSLKPEDTAVYSCAASQ APITIATMMKPFYDYWGQGTQVTVSS;
  • SEQ ID No: 395 of US 8,907,065 (SEQ ID NO:194): EVQLVESGGGLVQPGGSLRLSCAASGFTLDYYAKCWFRQAPGKEREWVSC ISSSDGSTYYADSVKGRFTISRDNAKNTVYLQMNSLKPEDTAVYFCAARH GGPLTVEYFFDYWGQGTQVTVSS :
  • SEQ ID No: 396 of US 8,907,065 (SEQ ID NO:195): EVQLVESGGGLVQPGGSLRLSCAASGFTFDYYAIGWFRQAPGKAREGVSC I SGGDNSTYYADSVKGRFTI SRDNAKNTVYLQMNSLKPEDTAVYYCATGG WKYCSGYDPEYIYWGQGTQVTVSS ;
  • SEQ ID No: 397 of US 8,907,065 (SEQ ID NO:196): EVQLVESGGGLVQAGGSLRLSCAASGSTFSQYDVGWYRQAPGKQRELVA FSSSGGRTIYPDSVKGRFTFSRDNTKNTVYLQMTSLKPEDTAVYYCKIDW YLNSYWGQGTQVTVSS ;
  • SEQ ID No: 399 of US 8,907,065 (SEQ ID NO:198): EVQLVESGGGLVQAGGSLTISCAASGITFSDSIVSWYRRARGKQREWVAG I SNGGTTKYAESVLGRFTI SRDNAKNNVYLQMNGLNPEDTAVYLCKVRQY WGQGTQVTVSS .
  • the present multi-specific CD8 binding agent has one or more targeting moieties directed against PD-L2.
  • the CD8 binding agent has one or more targeting moieties which selectively bind a PD-L2 polypeptide.
  • the CD8 binding agent comprises one or more antibodies, antibody derivatives or formats, peptides or polypeptides, or fusion proteins that selectively bind a PD-L2 polypeptide.
  • the targeting moiety comprises a VHH directed against PD-L2 as disclosed, for example, in US 8,907,065 and WO 2008/071447, the entire disclosures of which are hereby incorporated by reference.
  • the VHHs against PD-1 comprise SEQ ID Nos: 449-455 of US 8,907,065:
  • SEQ ID No: 449 of US 8,907,065 (SEQ ID NO:199): EVQLVESGGGLVQAGGSLRLSCAASESTVLINAMGWYRQAPGKQRELVAS I SSGGSTNYADSVKGRFTI SRDNAKNTVYLQMNSLKPEDTAVYYCNADVY PQDYGLGYVEGKVYYGHDYWGTGTLVTVSS;
  • SEQ ID No: 450 of US 8,907,065 (SEQ ID NO:200): EVQLVESGGGLVQAGGSLRLSCAASGSTFSNYVSNYAMGWGRQAPGTQ
  • SEQ ID No: 451 of US 8,907,065 (SEQ ID NO:201): EVQLVESGGGLVQAGGSLRLSCVASGXALKIXVMGWYRQAPGKQRELV AAITSGGRTNYSDSVKGRFTI SGDNAXNTVYLQMNSLKSEDTAVYYCRE WNSGYPPVDYWGQGTQVTVSS ;
  • SEQ ID No: 452 of US 8,907,065 (SEQ ID NO:202): EVQLVESGGGLVQAGGSLRLSCAASGRTFSSGTMGWFRRAPGKEREFV
  • SEQ ID No: 453 of US 8,907,065 (SEQ ID NO:203): EVQLVESGGGLVQTGGSLRLSCAASGFTLDYYGIGWFRQAPGKEREGVS FISGSDGSTYYAESVKGRFTISRDKAKNTVYLQMNSLKPEDTAVYYCAAD PWGPPSIATMTSYEYKHWGQGTQVTVSS ;
  • SEQ ID No: 454 of US 8,907,065 (SEQ ID NO:204): EVQLVESGGGLVQPGGSLRLSCAASGFTFSTYTMIWLRRAPGKGFEWV STIDKDGNTNYVDSVKGRFAVSRDNTKNTLYLQMNSLKPEDTAMYYCTK HGSSARGQGTRVTVSS;
  • SEQ ID No: 455 of US 8,907,065 SEQ ID NO:205: EVQLVESGGGLVEPGGSLRLSCVASGFTFSSYDMSWVRQAPGKGLE
  • the targeting moiety comprises any one of the anti-PD-L2 antibodies disclosed in US2011/0271358 and WO2010/036959, the entire contents of which are hereby incorporated by reference.
  • the antibody or an antigen-binding fragment thereof for use in the methods provided herein comprises a heavy chain comprising an amino acid sequence selected from SEQ ID Nos: 43-47 of US2011/0271358:
  • SEQ ID No: 47 of US2011/0271358 (SEQ ID NO:210): QVQLVQSGAEVKKPGASVKVSCKASGYTFTGYTMHWVRQAPGQGLEWIGYINPRSGYTEY NQKFKDRTTITADKSTSTAYMELSSLRSEDTAVYYCARPWFAYWGQGTLVTVSS;
  • a light chain comprising an amino acid sequence selected from SEQ ID Nos: 48-51 of US2011/0271358: SEQ ID No: 48 of US2011/0271358 (SEQ ID NO:211): DIVMTQSPASLTVTPGEKVTITCKSSQSLLNSGNQKNYLTWYQQKPGQPPKLLIYWASTR ESGVPDRFTGSGSGTDFTLTISSLQAEDVAVYYCQNDYSYPLTFGQGTKLEIK;
  • the targeting moieties of the invention may comprise a sequence that targets PD-1 , PD- L1 , and/or PD-L2 which is at least about 60%, at least about 61%, at least about 62%, at least about 63%, at least about 64%, at least about 65%, at least about 66%, at least about 67%, at least about 68%, at least about 69%, at least about 70%, at least about 71%, at least about 72%, at least about 73%, at least about 74%, at least about 75%, at least about 76%, at least about 77%, at least about 78%, at least about 79%, at least about 80%, at least about 81%, at least about 82%, at least about 83%, at least about 84%, at least about 85%, at least about 86%, at least about 87%, at least about 88%, at least about 89%, at least about 90%, at least about 91%, at least about 92%, at least about 93%, at least about 94%, at least about 95%,
  • the targeting moieties of the invention may comprise any combination of heavy chain, light chain, heavy chain variable region, light chain variable region, complementarity determining region (CDR), and framework region sequences that target PD-1 , PD-L1 , and/or PD-L2 as disclosed herein.
  • CDR complementarity determining region
  • Additional antibodies, antibody derivatives or formats, peptides or polypeptides, or fusion proteins that selectively bind or target PD-1 , PD-L1 and/or PD-L2 are disclosed in WO 2011/066389, US 2008/0025980, US 2013/0034559, US 8,779,108, US 2014/0356353, US 8,609,089, US 2010/028330, US 2012/0114649, WO 2010/027827, WO 2011./066342, US 8,907,065, WO 2016/062722, WO 2009/101611 , WO2010/027827, WO 2011/066342, WO 2007/005874 , WO 2001/014556, US2011/0271358, WO 2010/036959, WO 2010/077634, US 8,217,149, US 2012/0039906, WO 2012/145493, US 2011/0318373, U.S.
  • the multi-specific CD8 binding agent of the invention comprises a targeting moiety having an antigen recognition domain that specifically binds to XCR1 , e.g. on DCs. In various embodiments, the multi-specific CD8 binding agent of the invention comprises a targeting moiety having an antigen recognition domain that comprise all of or part of XCL1.
  • the multi-specific CD8 binding agents have targeting moieties having recognition domains which specifically bind to a target (e.g. antigen, receptor) which is part of a non-cellular structure.
  • a target e.g. antigen, receptor
  • the antigen or receptor is not an integral component of an intact cell or cellular structure.
  • the antigen or receptor is an extracellular antigen or receptor.
  • the target is a non-proteinaceous, non-cellular marker, including, without limitation, nucleic acids, inclusive of DNA or RNA, such as, for example, DNA released from necrotic tumor cells or extracellular deposits such as cholesterol.
  • the target (e.g. antigen, receptor) of interest is part of the non-cellular component of the stroma or the extracellular matrix (ECM) or the markers associated therewith.
  • stroma refers to the connective and supportive framework of a tissue or organ. Stroma may include a compilation of cells such as fibroblasts/myofibroblasts, glial, epithelia, fat, immune, vascular, smooth muscle, and immune cells along with the extracellular matrix (ECM) and extracellular molecules.
  • the target (e.g. antigen, receptor) of interest is part of the non-cellular component of the stroma such as the extracellular matrix and extracellular molecules.
  • the ECM refers to the non-cellular components present within all tissues and organs.
  • the ECM is composed of a large collection of biochemically distinct components including, without limitation, proteins, glycoproteins, proteoglycans, and polysaccharides. These components of the ECM are usually produced by adjacent cells and secreted into the ECM via exocytosis. Once secreted, the ECM components often aggregate to form a complex network of macromolecules.
  • the chimeric protein of the invention comprises a targeting moiety that recognizes a target (e.g., an antigen or receptor or non-proteinaceous molecule) located on any component of the ECM.
  • Illustrative components of the ECM include, without limitation, the proteoglycans, the non-proteoglycan polysaccharides, fibers, and other ECM proteins or ECM non-proteins, e.g. polysaccharides and/or lipids, or ECM associated molecules (e.g. proteins or non-proteins, e.g. polysaccharides, nucleic acids and/or lipids).
  • the targeting moiety recognizes a target (e.g. antigen, receptor) on ECM proteoglycans.
  • Proteoglycans are glycosylated proteins.
  • the basic proteoglycan unit includes a core protein with one or more covalently attached glycosaminoglycan (GAG) chains.
  • GAG glycosaminoglycan chains.
  • Proteoglycans have a net negative charge that attracts positively charged sodium ions (Na+), which attracts water molecules via osmosis, keeping the ECM and resident cells hydrated. Proteoglycans may also help to trap and store growth factors within the ECM.
  • Illustrative proteoglycans that may be targeted by the chimeric proteins of the invention include, but are not limited to, heparan sulfate, chondroitin sulfate, and keratan sulfate.
  • the targeting moiety recognizes a target (e.g. antigen, receptor) on non-proteoglycan polysaccharides such as hyaluronic acid.
  • the targeting moiety recognizes a target (e.g. antigen, receptor) on ECM fibers.
  • ECM fibers include collagen fibers and elastin fibers.
  • the targeting moiety recognizes one or more epitopes on collagens or collagen fibers.
  • Collagens are the most abundant proteins in the ECM. Collagens are present in the ECM as fibrillar proteins and provide structural support to resident cells.
  • the targeting moiety recognizes and binds to various types of collagens present within the ECM including, without limitation, fibrillar collagens (types I, II, III, V, XI), facit collagens (types IX, XII, XIV), short chain collagens (types VIII, X), basement membrane collagens (type IV), and/or collagen types VI, VII, or XIII.
  • Elastin fibers provide elasticity to tissues, allowing them to stretch when needed and then return to their original state.
  • the target moiety recognizes one or more epitopes on elastins or elastin fibers.
  • the targeting moiety recognizes one or more ECM proteins including, but not limited to, a tenascin, a fibronectin, a fibrin, a laminin, or a nidogen/entactin.
  • the targeting moiety recognizes and binds to tenascin.
  • the tenascin (TN) family of glycoproteins includes at least four members, tenascin-C, tenascin-R, tenascin-X, and tenascin W.
  • the primary structures of tenascin proteins include several common motifs ordered in the same consecutive sequence: amino-terminal heptad repeats, epidermal growth factor (EGF)-like repeats, fibronectin type III domain repeats, and a carboxyl-terminal fibrinogen-like globular domain. Each protein member is associated with typical variations in the number and nature of EGF-like and fibronectin type III repeats.
  • Isoform variants also exist particularly with respect to tenascin-C. Over 27 splice variants and/or isoforms of tenascin-C are known. In a particular embodiment, the targeting moiety recognizes and binds to tenascin-CA1. Similarly, tenascin-R also has various splice variants and isoforms. Tenascin-R usually exists as dimers or trimers. Tenascin-X is the largest member of the tenascin family and is known to exist as trimers. Tenascin-W exists as trimers. In some embodiments, the targeting moiety recognizes one or more epitopes on a tenascin protein. In some embodiments, the targeting moiety recognizes the monomeric and/or the dimeric and/or the trimeric and/or the hexameric forms of a tenascin protein.
  • the targeting moieties recognize and bind to fibronectin.
  • Fibronectins are glycoproteins that connect cells with collagen fibers in the ECM, allowing cells to move through the ECM. Upon binding to integrins, fibronectins unfolds to form functional dimers.
  • the targeting moiety recognizes the monomeric and/or the dimeric forms of fibronectin.
  • the targeting moiety recognizes one or more epitopes on fibronectin.
  • the targeting moiety recognizes fibronectin extracellular domain A (EDA) or fibronectin extracellular domain B (EDB).
  • Elevated levels of EDA are associated with various diseases and disorders including psoriasis, rheumatoid arthritis, diabetes, and cancer.
  • the targeting moiety recognizes fibronectin that contains the EDA isoform and may be utilized to target the chimeric protein to diseased cells including cancer cells.
  • the targeting moiety recognizes fibronectin that contains the EDB isoform.
  • such targeting moieties may be utilized to target the chimeric protein to tumor cells including the tumor neovasculature.
  • the targeting moiety recognizes and binds to fibrin.
  • Fibrin is another protein substance often found in the matrix network of the ECM. Fibrin is formed by the action of the protease thrombin on fibrinogen which causes the fibrin to polymerize.
  • the targeting moiety recognizes one or more epitopes on fibrin. In some embodiments, the targeting moiety recognizes the monomeric as well as the polymerized forms of fibrin.
  • the targeting moiety recognizes and binds to laminin.
  • Laminin is a major component of the basal lamina, which is a protein network foundation for cells and organs.
  • Laminins are heterotrimeric proteins that contain an a-chain, a ⁇ -chain, and a ⁇ -chain.
  • the targeting moiety recognizes one or more epitopes on laminin.
  • the targeting moiety recognizes the monomeric, the dimeric as well as the trimeric forms of laminin.
  • the targeting moiety recognizes and binds to a nidogen or entactin.
  • Nidogens/entactins are a family of highly conserved, sulfated glycoproteins. They make up the major structural component of the basement membranes and function to link laminin and collagen IV networks in basement membranes. Members of this family include nidogen-1 and nidogen-2.
  • the targeting moiety recognizes an epitope on nidogen-1 and/or nidogen-2.
  • the targeting moiety comprises an antigen recognition domain that recognizes an epitope present on any of the targets (e.g., ECM proteins) described herein.
  • the antigen- recognition domain recognizes one or more linear epitopes present on the protein.
  • a linear epitope refers to any continuous sequence of amino acids present on the protein.
  • the antigen-recognition domain recognizes one or more conformational epitopes present on the protein.
  • a conformation epitope refers to one or more sections of amino acids (which may be discontinuous) which form a three-dimensional surface with features and/or shapes and/or tertiary structures capable of being recognized by an antigen recognition domain.
  • the targeting moiety may bind to the full-length and/or mature forms and/or isoforms and/or splice variants and/or fragments and/or any other naturally occurring or synthetic analogs, variants, or mutants of any of the targets (e.g., ECM proteins) described herein.
  • the targeting moiety may bind to any forms of the proteins described herein, including monomeric, dimeric, trimeric, tetrameric, heterodimeric, multimeric and associated forms.
  • the targeting moiety may bind to any post-translationally modified forms of the proteins described herein, such as glycosylated and/or phosphorylated forms.
  • the targeting moiety comprises an antigen recognition domain that recognizes extracellular molecules such as DNA. In some embodiments, the targeting moiety comprises an antigen recognition domain that recognizes DNA. In an embodiment, the DNA is shed into the extracellular space from necrotic or apoptotic tumor cells or other diseased cells.
  • the targeting moiety comprises an antigen recognition domain that recognizes one or more non-cellular structures associated with atherosclerotic plaques.
  • the fibro-lipid (fibro-fatty) plaque is characterized by an accumulation of lipid-laden cells underneath the intima of the arteries. Beneath the endothelium there is a fibrous cap covering the atheromatous core of the plaque.
  • the core includes lipid-laden cells (macrophages and smooth muscle cells) with elevated tissue cholesterol and cholesterol ester content, fibrin, proteoglycans, collagen, elastin, and cellular debris.
  • the central core of the plaque usually contains extracellular cholesterol deposits (released from dead cells), which form areas of cholesterol crystals with empty, needle-like clefts.
  • extracellular cholesterol deposits released from dead cells
  • a fibrous plaque is also localized under the intima, within the wall of the artery resulting in thickening and expansion of the wall and, sometimes, spotty localized narrowing of the lumen with some atrophy of the muscular layer.
  • the fibrous plaque contains collagen fibers (eosinophilic), precipitates of calcium (hematoxylinophilic) and lipid-laden cells.
  • the targeting moiety recognizes and binds to one or more of the non-cellular components of these plaques such as the fibrin, proteoglycans, collagen, elastin, cellular debris, and calcium or other mineral deposits or precipitates.
  • the cellular debris is a nucleic acid, e.g. DNA or RNA, released from dead cells.
  • the targeting moiety comprises an antigen recognition domain that recognizes one or more non-cellular structures found in the brain plaques associated with neurodegenerative diseases.
  • the targeting moiety recognizes and binds to one or more non-cellular structures located in the amyloid plaques found in the brains of patients with Alzheimer's disease.
  • the targeting moiety may recognize and bind to the peptide amyloid beta, which is a major component of the amyloid plaques.
  • the targeting moiety recognizes and binds to one or more non-cellular structures located in the brains plaques found in patients with Huntington's disease.
  • the targeting moiety recognizes and binds to one or more non-cellular structures found in plaques associated with other neurodegenerative or musculoskeletal diseases such as Lewy body dementia and inclusion body myositis.
  • the CD8 binding agent may include one or more functional groups, residues, or moieties.
  • the one or more functional groups, residues, or moieties are attached or genetically fused to any of the signaling agents or targeting moieties described herein.
  • such functional groups, residues or moieties confer one or more desired properties or functionalities to the CD8 binding agent of the invention. Examples of such functional groups and of techniques for introducing them into the CD8 binding agent are known in the art, for example, see Remington's Pharmaceutical Sciences, 16th ed., Mack Publishing Co., Easton, Pa. (1980).
  • the CD8 binding agent may by conjugated and/or fused with another agent to extend half-life or otherwise improve pharmacodynamic and pharmacokinetic properties.
  • the CD8 binding agent may be fused or conjugated with one or more of PEG, XTEN (e.g., as rPEG), polysialic acid (POLYXEN), albumin [e.g., human serum albumin or HAS), elastin-like protein (ELP), PAS, HAP, GLK, CTP, transferrin, and the like.
  • the CD8 binding agent may be fused or conjugated with an antibody or an antibody fragment such as an Fc fragment.
  • the chimeric protein may be fused to either the N-terminus or the C-terminus of the Fc domain of human immunoglobulin (Ig) G.
  • each of the individual chimeric proteins is fused to one or more of the agents described in BioDrugs (2015) 29:215-239, the entire contents of which are hereby incorporated by reference.
  • the functional groups, residues, or moieties comprise a suitable pharmacologically acceptable polymer, such as poly(ethyleneglycol) (PEG) or derivatives thereof (such as methoxypoly(ethyleneglycol) or mPEG).
  • PEG poly(ethyleneglycol)
  • attachment of the PEG moiety increases the half-life and/or reduces the immunogenecity of the CD8 binding protein.
  • any suitable form of pegylation can be used, such as the pegylation used in the art for antibodies and antibody fragments (including but not limited to single domain antibodies such as VHHs); see, for example, Chapman, Nat. Biotechnol., 54, 531-545 (2002); by Veronese and Harris, Adv. Drug Deliv. Rev.
  • reagents for pegylation of proteins are also commercially available, for example, from Nektar Therapeutics, USA.
  • site-directed pegylation is used, in particular via a cysteine-residue (see, for example, Yang ef a/., Protein Engineering, 16, 10, 761-770 (2003), the entire contents of which is hereby incorporated by reference).
  • PEG may be attached to a cysteine residue that naturally occurs in the CD8 binding agent of the invention.
  • the CD8 binding agent of the invention is modified so as to suitably introduce one or more cysteine residues for attachment of PEG, or an amino acid sequence comprising one or more cysteine residues for attachment of PEG may be fused to the amino- and/or carboxy-terminus of the CD8 binding agent, using techniques known in the art.
  • the functional groups, residues, or moieties comprise N-linked or O-linked glycosylation.
  • the N-linked or O-linked glycosylation is introduced as part of a co-translational and/or post- translational modification.
  • the functional groups, residues, or moieties comprise one or more detectable labels or other signal-generating groups or moieties.
  • Suitable labels and techniques for attaching, using and detecting them are known in the art and, include, but are not limited to, fluorescent labels (such as fluorescein, isothiocyanate, rhodamine, phycoerythrin, phycocyanin, allophycocyanin, o-phthaldehyde, and fluorescamine and fluorescent metals such as Eu or others metals from the lanthanide series), phosphorescent labels, chemiluminescent labels or bioluminescent labels (such as luminal, isoluminol, theromatic acridinium ester, imidazole, acridinium salts, oxalate ester, dioxetane or GFP and its analogs), radio-isotopes, metals, metals chelates or metallic cations or other metals or metallic cations that are particularly suited
  • VHHs and polypeptides of the invention may, for example, be used for in vitro, in vivo or in situ assays (including immunoassays known per se such as ELISA, RIA, EIA and other "sandwich assays," etc.) as well as in vivo diagnostic and imaging purposes, depending on the choice of the specific label.
  • the functional groups, residues, or moieties comprise a tag that is attached or genetically fused to the CD8 binding agent.
  • the CD8 binding agent may include a single tag or multiple tags.
  • the tag for example is a peptide, sugar, or DNA molecule that does not inhibit or prevent binding of the CD8 binding agent to CD8 or any other antigen of interest such as tumor antigens.
  • the tag is at least about: three to five amino acids long, five to eight amino acids long, eight to twelve amino acids long, twelve to fifteen amino acids long, or fifteen to twenty amino acids long. Exemplary tags are described for example, in U.S. Patent Publication No. US2013/0058962.
  • the tag is an affinity tag such as glutathione-S-transferase (GST) and histidine (His) tag.
  • the CD8 binding agent comprises a His tag.
  • the functional groups, residues, or moieties comprise a chelating group, for example, to chelate one of the metals or metallic cations.
  • Suitable chelating groups include, without limitation, diethyl-enetriaminepentaacetic acid (DTPA) or ethylenediaminetetraacetic acid (EDTA).
  • the functional groups, residues, or moieties comprise a functional group that is one part of a specific binding pair, such as the biotin-(strept)avidin binding pair.
  • a functional group may be used to link the CD8 binding agent of the invention to another protein, polypeptide or chemical compound that is bound to the other half of the binding pair, i.e., through formation of the binding pair.
  • a CD8 binding agent of the invention may be conjugated to biotin, and linked to another protein, polypeptide, compound or carrier conjugated to avidin or streptavidin.
  • such a conjugated CD8 binding agent may be used as a reporter, for example, in a diagnostic system where a detectable signal-producing agent is conjugated to avidin or streptavidin.
  • binding pairs may, for example, also be used to bind the CD8 binding agent to a carrier, including carriers suitable for pharmaceutical purposes.
  • carriers suitable for pharmaceutical purposes include carriers suitable for pharmaceutical purposes.
  • One non-limiting example are the liposomal formulations described by Cao and Suresh, Journal of Drug Targeting, 8, 4, 257 (2000).
  • Such binding pairs may also be used to link a therapeutically active agent to the CD8 binding agent of the invention.
  • the present CD8 binding agent optionally comprises one or more linkers.
  • the present CD8 binding agent comprises a linker connecting the targeting moiety and the signaling agent.
  • the present chimeric protein comprises a linker within the signaling agent (e.g. in the case of single chain TNF, which can comprise two linkers to yield a trimer).
  • the CD8 binding agent includes a linker that connects each binding region and/or targeting moieties.
  • the linker may be utilized to link various functional groups, residues, or moieties as described herein to the CD8 binding agent.
  • the linker is a single amino acid or a plurality of amino acids that does not affect or reduce the stability, orientation, binding, neutralization, and/or clearance characteristics of the binding regions and the binding protein.
  • the linker is selected from a peptide, a protein, a sugar, or a nucleic acid.
  • the linker may be derived from naturally-occurring multi-domain proteins or are empirical linkers as described, for example, in Chichili et al., (2013), Protein Sci. 22(2): 153-167, Chen et al., (2013), Adv Drug Deliv Rev. 65(10): 1357-1369, the entire contents of which are hereby incorporated by reference.
  • the linker may be designed using linker designing databases and computer programs such as those described in Chen ef a/., (2013), Adv Drug Deliv Rev. 65(10):1357-1369 and Crasto ef a/., (2000), Protein Eng.
  • the linker may be functional.
  • the linker may function to improve the folding and/or stability, improve the expression, improve the pharmacokinetics, and/or improve the bioactivity of the present CD8 binding agent.
  • the linker is a polypeptide. In some embodiments, the linker is less than about 100 amino acids long.
  • the linker may be less than about 100, about 95, about 90, about 85, about 80, about 75, about 70, about 65, about 60, about 55, about 50, about 45, about 40, about 35, about 30, about 25, about 20, about 19, about 18, about 17, about 16, about 15, about 14, about 13, about 12, about 11 , about 10, about 9, about 8, about 7, about 6, about 5, about 4, about 3, or about 2 amino acids long.
  • the linker is flexible. In another embodiment, the linker is rigid.
  • the linker is a polypeptide. In some embodiments, the linker is greater than about 100 amino acids long. For example, the linker may be greater than about 100, about 95, about 90, about 85, about 80, about 75, about 70, about 65, about 60, about 55, about 50, about 45, about 40, about 35, about 30, about 25, about 20, about 19, about 18, about 17, about 16, about 15, about 14, about 13, about 12, about 11 , about 10, about 9, about 8, about 7, about 6, about 5, about 4, about 3, or about 2 amino acids long. In some embodiments, the linker is flexible. In another embodiment, the linker is rigid.
  • the linker is substantially comprised of glycine and serine residues (e.g. about 30%, or about 40%, or about 50%, or about 60%, or about 70%, or about 80%, or about 90%, or about 95%, or about 97% glycines and serines).
  • the linker is (Gly4Ser) n , where n is from about 1 to about 8, e.g. 1 , 2, 3, 4, 5, 6, 7, or 8.
  • the linker sequence is GGSGGSGGGGSGGGGS (SEQ ID NO:215).
  • the linker is a hinge region of an antibody (e.g., of IgG, IgA, IgD, and IgE, inclusive of subclasses (e.g. lgG1 , lgG2, lgG3, and lgG4, and lgA1 and lgA2)).
  • the linker is a hinge region of an antibody (e.g., of IgG, IgA, IgD, and IgE, inclusive of subclasses (e.g. lgG1 , lgG2, lgG3, and lgG4, and lgA1 and lgA2)).
  • the hinge region acts as a flexible spacer, allowing the Fab portion to move freely in space.
  • the hinge domains are structurally diverse, varying in both sequence and length among immunoglobulin classes and subclasses. For example, the length and flexibility of the hinge region varies among the IgG subclasses.
  • the hinge region of lgG1 encompasses amino acids 216-231 and, because it is freely flexible, the Fab fragments can rotate about their axes of symmetry and move within a sphere centered at the first of two inter-heavy chain disulfide bridges.
  • lgG2 has a shorter hinge than lgG1 , with 12 amino acid residues and four disulfide bridges.
  • the hinge region of lgG2 lacks a glycine residue, is relatively short, and contains a rigid poly-proline double helix, stabilized by extra inter-heavy chain disulfide bridges. These properties restrict the flexibility of the lgG2 molecule.
  • lgG3 differs from the other subclasses by its unique extended hinge region (about four times as long as the lgG1 hinge), containing 62 amino acids (including 21 prolines and 11 cysteines), forming an inflexible poly-proline double helix.
  • the Fab fragments are relatively far away from the Fc fragment, giving the molecule a greater flexibility.
  • the elongated hinge in lgG3 is also responsible for its higher molecular weight compared to the other subclasses.
  • the hinge region of lgG4 is shorter than that of lgG1 and its flexibility is intermediate between that of lgG1 and lgG2.
  • the flexibility of the hinge regions reportedly decreases in the order lgG3>lgG1 >lgG4>lgG2.
  • the immunoglobulin hinge region can be further subdivided functionally into three regions: the upper hinge region, the core region, and the lower hinge region.
  • the upper hinge region includes amino acids from the carboxyl end of Cm to the first residue in the hinge that restricts motion, generally the first cysteine residue that forms an interchain disulfide bond between the two heavy chains.
  • the length of the upper hinge region correlates with the segmental flexibility of the antibody.
  • the core hinge region contains the inter-heavy chain disulfide bridges, and the lower hinge region joins the amino terminal end of the CH2 domain and includes residues in CH2. Id.
  • the core hinge region of wild-type human lgG1 contains the sequence Cys-Pro-Pro-Cys which, when dimerized by disulfide bond formation, results in a cyclic octapeptide believed to act as a pivot, thus conferring flexibility.
  • the present linker comprises, one, or two, or three of the upper hinge region, the core region, and the lower hinge region of any antibody (e.g., of IgG, IgA, IgD, and IgE, inclusive of subclasses (e.g. lgG1 , lgG2, lgG3, and lgG4, and lgA1 and lgA2)).
  • the hinge region may also contain one or more glycosylation sites, which include a number of structurally distinct types of sites for carbohydrate attachment.
  • lgA1 contains five glycosylation sites within a 17-amino-acid segment of the hinge region, conferring resistance of the hinge region polypeptide to intestinal proteases, considered an advantageous property for a secretory immunoglobulin.
  • the linker of the present invention comprises one or more glycosylation sites.
  • the linker is a hinge-CH2-CH3 domain of a human lgG4 antibody.
  • the present CD8 binding agent can be linked to an antibody Fc region, comprising one or both of CH2 and CH3 domains, and optionally a hinge region.
  • vectors encoding the present CD8 binding agents linked as a single nucleotide sequence to an Fc region can be used to prepare such polypeptides.
  • the linker is a synthetic linker such as PEG.
  • the linker may be functional.
  • the linker may function to improve the folding and/or stability, improve the expression, improve the pharmacokinetics, and/or improve the bioactivity of the present CD8 binding agent.
  • the linker may function to target the CD8 binding agent to a particular cell type or location. Modifications and Production of CD8 Binding Agents
  • the CD8 binding agent comprises a targeting moiety that is a VHH.
  • the VHH is not limited to a specific biological source or to a specific method of preparation.
  • the VHH can generally be obtained: (1) by isolating the VHH domain of a naturally occurring heavy chain antibody; (2) by expression of a nucleotide sequence encoding a naturally occurring VHH domain; (3) by "humanization” of a naturally occurring VHH domain or by expression of a nucleic acid encoding a such humanized VHH domain; (4) by "camelization” of a naturally occurring VH domain from any animal species, such as from a mammalian species, such as from a human being, or by expression of a nucleic acid encoding such a camelized VH domain; (5) by "camelization” of a "domain antibody” or “Dab” as described in the art, or by expression of a nucleic acid encoding such a camelized VH domain; (6) by using
  • the CD8 binding agent comprises a VHH that corresponds to the VHH domains of naturally occurring heavy chain antibodies directed against human CD8.
  • VHH sequences can generally be generated or obtained by suitably immunizing a species of Camel id with a CD8 molecule, (i.e., so as to raise an immune response and/or heavy chain antibodies directed against CD8), by obtaining a suitable biological sample from the Camelid (such as a blood sample, or any sample of B-cells), and by generating VHH sequences directed against CD8, starting from the sample, using any suitable known techniques.
  • naturally occurring VHH domains against CD8 can be obtained from naive libraries of Camelid VHH sequences, for example, by screening such a library using CD8 or at least one part, fragment, antigenic determinant or epitope thereof using one or more screening techniques known in the art.
  • Such libraries and techniques are, for example, described in W09937681 , WO0190190, WO03025020 and WO03035694, the entire contents of which are hereby incorporated by reference.
  • improved synthetic or semi-synthetic libraries derived from naive VHH libraries may be used, such as VHH libraries obtained from naive VHH libraries by techniques such as random mutagenesis and/or CDR shuffling, as for example, described in WO0043507, the entire contents of which are hereby incorporated by reference.
  • another technique for obtaining VHH sequences directed against a CD8 involves suitably immunizing a transgenic mammal that is capable of expressing heavy chain antibodies (i.e., so as to raise an immune response and/or heavy chain antibodies directed against CD8), obtaining a suitable biological sample from the transgenic mammal (such as a blood sample, or any sample of B-cells), and then generating VHH sequences directed against CD8 starting from the sample, using any suitable known techniques.
  • a suitable biological sample such as a blood sample, or any sample of B-cells
  • VHH sequences directed against CD8 starting from the sample, using any suitable known techniques.
  • the heavy chain antibody-expressing mice and the further methods and techniques described in WO02085945 and in WO04049794 can be used.
  • the CD8 binding agent comprises a VHH that has been "humanized” i.e., by replacing one or more amino acid residues in the amino acid sequence of the naturally occurring VHH sequence (and in particular in the framework sequences) by one or more of the amino acid residues that occur at the corresponding position(s) in a VH domain from a conventional 4-chain antibody from a human being.
  • VHH has been "humanized” i.e., by replacing one or more amino acid residues in the amino acid sequence of the naturally occurring VHH sequence (and in particular in the framework sequences) by one or more of the amino acid residues that occur at the corresponding position(s) in a VH domain from a conventional 4-chain antibody from a human being.
  • humanization techniques known in the art.
  • possible humanizing substitutions or combinations of humanizing substitutions may be determined by methods known in the art, for example, by a comparison between the sequence of a VHH and the sequence of a naturally occurring human VH domain.
  • the humanizing substitutions are chosen such that the resulting humanized VHHs still retain advantageous functional properties.
  • the VHHs of the invention may become more "human-like," while still retaining favorable properties such as a reduced immunogenicity, compared to the corresponding naturally occurring VHH domains.
  • the humanized VHHs of the invention can be obtained in any suitable manner known in the art and thus are not strictly limited to polypeptides that have been obtained using a polypeptide that comprises a naturally occurring VHH domain as a starting material.
  • the CD8 binding agent comprises a VHH that has been "camelized,” i.e., by replacing one or more amino acid residues in the amino acid sequence of a naturally occurring VH domain from a conventional 4- chain antibody by one or more of the amino acid residues that occur at the corresponding position(s) in a VHH domain of a heavy chain antibody of a camelid.
  • VHH that has been "camelized,” i.e., by replacing one or more amino acid residues in the amino acid sequence of a naturally occurring VH domain from a conventional 4- chain antibody by one or more of the amino acid residues that occur at the corresponding position(s) in a VHH domain of a heavy chain antibody of a camelid.
  • such "camelizing" substitutions are inserted at amino acid positions that form and/or are present at the VH-VL interface, and/or at the so-called Camelidae hallmark residues (see, for example, WO9404678, the entire contents of which are hereby incorporated by reference).
  • the VH sequence that is used as a starting material or starting point for generating or designing the camelized VHH is a VH sequence from a mammal, for example, the VH sequence of a human being, such as a VH3 sequence.
  • the camelized VHHs can be obtained in any suitable manner known in the art (i.e., as indicated under points (1)-(8) above) and thus are not strictly limited to polypeptides that have been obtained using a polypeptide that comprises a naturally occurring VH domain as a starting material.
  • both "humanization” and “camelization” can be performed by providing a nucleotide sequence that encodes a naturally occurring VHH domain or VH domain, respectively, and then changing, in a manner known in the art, one or more codons in the nucleotide sequence in such a way that the new nucleotide sequence encodes a "humanized” or “camelized” VHH, respectively.
  • This nucleic acid can then be expressed in a manner known in the art, so as to provide the desired VHH of the invention.
  • the amino acid sequence of the desired humanized or camelized VHH of the invention can be designed and then synthesized de novo using techniques for peptide synthesis known in the art.
  • a nucleotide sequence encoding the desired humanized or camelized VHH can be designed and then synthesized de novo using techniques for nucleic acid synthesis known in the art, after which the nucleic acid thus obtained can be expressed in a manner known in the art, so as to provide the desired VHH of the invention.
  • VHHs of the invention and/or nucleic acids encoding the same starting from naturally occurring VH sequences or VHH sequences, are known in the art, and may, for example, comprise combining one or more parts of one or more naturally occurring VH sequences (such as one or more FR sequences and/or CDR sequences), one or more parts of one or more naturally occurring VHH sequences (such as one or more FR sequences or CDR sequences), and/or one or more synthetic or semi-synthetic sequences, in a suitable manner, so as to provide a VHH of the invention or a nucleotide sequence or nucleic acid encoding the same.
  • DNA sequences encoding the CD8 binding agents of the invention can be chemically synthesized using methods known in the art. Synthetic DNA sequences can be ligated to other appropriate nucleotide sequences, including, e.g., expression control sequences, to produce gene expression constructs encoding the desired CD8 binding agents. Accordingly, in various embodiments, the present invention provides for isolated nucleic acids comprising a nucleotide sequence encoding the CD8 binding agent of the invention.
  • Nucleic acids encoding the CD8 binding agent of the invention can be incorporated (ligated) into expression vectors, which can be introduced into host cells through transfection, transformation, or transduction techniques.
  • nucleic acids encoding the CD8 binding agent of the invention can be introduced into host cells by retroviral transduction.
  • Illustrative host cells are E.coli cells, Chinese hamster ovary (CHO) cells, human embryonic kidney 293 (HEK 293) cells, HeLa cells, baby hamster kidney (BHK) cells, monkey kidney cells (COS), human hepatocellular carcinoma cells (e.g., Hep G2), and myeloma cells.
  • Transformed host cells can be grown under conditions that permit the host cells to express the genes that encode the CD8 binding agent of the invention. Accordingly, in various embodiments, the present invention provides expression vectors comprising nucleic acids that encode the CD8 binding agent of the invention. In various embodiments, the present invention additional provides host cells comprising such expression vectors.
  • a gene is to be expressed in E. coli, it is first cloned into an expression vector by positioning the engineered gene downstream from a suitable bacterial promoter, e.g., Trp or Tac, and a prokaryotic signal sequence.
  • a suitable bacterial promoter e.g., Trp or Tac
  • a prokaryotic signal sequence e.g., Trp or Tac
  • the engineered gene is to be expressed in eukaryotic host cells, e.g., CHO cells, it is first inserted into an expression vector containing for example, a suitable eukaryotic promoter, a secretion signal, enhancers, and various introns.
  • the gene construct can be introduced into the host cells using transfection, transformation, or transduction techniques.
  • the CD8 binding agent of the invention can be produced by growing a host cell transfected with an expression vector encoding the CD8 binding agent under conditions that permit expression of the protein. Following expression, the protein can be harvested and purified using techniques well known in the art, e.g., affinity tags such as glutathione-S-transferase (GST) and histidine (His) tags or by chromatography.
  • affinity tags such as glutathione-S-transferase (GST) and histidine (His) tags or by chromatography.
  • the CD8 binding agent comprises a His tag (which is optionally cleavable via an engineered proteolytic cleavage site).
  • the present invention provides for a nucleic acid encoding a CD8 binding agent of the present invention.
  • the present invention provides for a host cell comprising a nucleic acid encoding a CD8 binding agent of the present invention.
  • the CD8 binding agents described herein can possess a sufficiently basic functional group, which can react with an inorganic or organic acid, or a carboxyl group, which can react with an inorganic or organic base, to form a pharmaceutically acceptable salt.
  • a pharmaceutically acceptable acid addition salt is formed from a pharmaceutically acceptable acid, as is well known in the art.
  • Such salts include the pharmaceutically acceptable salts listed in, for example, Journal of Pharmaceutical Science, 66, 2-19 (1977) and The Handbook of Pharmaceutical Salts; Properties, Selection, and Use. P. H. Stahl and C. G. Wermuth (eds.), Verlag, Zurich (Switzerland) 2002, which are hereby incorporated by reference in their entirety.
  • salts include, by way of non-limiting example, sulfate, citrate, acetate, oxalate, chloride, bromide, iodide, nitrate, bisulfate, phosphate, acid phosphate, isonicotinate, lactate, salicylate, acid citrate, tartrate, oleate, tannate, pantothenate, bitartrate, ascorbate, succinate, maleate, gentisinate, fumarate, gluconate, glucaronate, saccharate, formate, benzoate, glutamate, methanesulfonate, ethanesulfonate, benzenesulfonate, p-toluenesulfonate, camphorsulfonate, pamoate, phenylacetate, trifluoroacetate, acrylate, chlorobenzoate, dinitrobenzoate, hydroxybenzoate, methoxybenzoate, methylbenzo
  • Suitable bases include, but are not limited to, hydroxides of alkali metals such as sodium, potassium, and lithium; hydroxides of alkaline earth metal such as calcium and magnesium; hydroxides of other metals, such as aluminum and zinc; ammonia, and organic amines, such as unsubstituted or hydroxy-substituted mono-, di-, or tri-alkylamines, dicyclohexylamine; tributyl amine; pyridine; N-methyl, N-ethylamine; diethylamine; triethylamine; mono-, bis-, or tris-(2-OH-lower alkylamines), such as mono-; bis-, or tris-(2-hydroxyethyl)amine, 2-hydroxy-tert-butylamine, or tris-(hydroxy
  • the present invention pertains to pharmaceutical compositions comprising the CD8 binding agents described herein and a pharmaceutically acceptable carrier or excipient.
  • Any pharmaceutical compositions described herein can be administered to a subject as a component of a composition that comprises a pharmaceutically acceptable carrier or vehicle.
  • Such compositions can optionally comprise a suitable amount of a pharmaceutically acceptable excipient so as to provide the form for proper administration.
  • pharmaceutical excipients can be liquids, such as water and oils, including those of petroleum, animal, vegetable, or synthetic origin, such as peanut oil, soybean oil, mineral oil, sesame oil and the like.
  • the pharmaceutical excipients can be, for example, saline, gum acacia, gelatin, starch paste, talc, keratin, colloidal silica, urea and the like.
  • auxiliary, stabilizing, thickening, lubricating, and coloring agents can be used.
  • the pharmaceutically acceptable excipients are sterile when administered to a subject. Water is a useful excipient when any agent described herein is administered intravenously.
  • Saline solutions and aqueous dextrose and glycerol solutions can also be employed as liquid excipients, specifically for injectable solutions.
  • suitable pharmaceutical excipients also include starch, glucose, lactose, sucrose, gelatin, malt, rice, flour, chalk, silica gel, sodium stearate, glycerol monostearate, talc, sodium chloride, dried skim milk, glycerol, propylene, glycol, water, ethanol and the like. Any agent described herein, if desired, can also comprise minor amounts of wetting or emulsifying agents, or pH buffering agents. Other examples of suitable pharmaceutical excipients are described in Remington's Pharmaceutical Sciences 1447-1676 (Alfonso R. Gennaro eds., 19th ed. 1995), incorporated herein by reference.
  • the present invention includes the described pharmaceutical compositions (and/or additional therapeutic agents) in various formulations.
  • Any inventive pharmaceutical composition (and/or additional therapeutic agents) described herein can take the form of solutions, suspensions, emulsion, drops, tablets, pills, pellets, capsules, capsules containing liquids, gelatin capsules, powders, sustained-release formulations, suppositories, emulsions, aerosols, sprays, suspensions, lyophilized powder, frozen suspension, dessicated powder, or any other form suitable for use.
  • the composition is in the form of a capsule.
  • the composition is in the form of a tablet.
  • the pharmaceutical composition is formulated in the form of a soft-gel capsule.
  • the pharmaceutical composition is formulated in the form of a gelatin capsule.
  • the pharmaceutical composition is formulated as a liquid.
  • the inventive pharmaceutical compositions (and/or additional agents) can also include a solubilizing agent.
  • the agents can be delivered with a suitable vehicle or delivery device as known in the art. Combination therapies outlined herein can be co-delivered in a single delivery vehicle or delivery device.
  • compositions comprising the inventive pharmaceutical compositions (and/or additional agents) of the present invention may conveniently be presented in unit dosage forms and may be prepared by any of the methods well known in the art of pharmacy. Such methods generally include the step of bringing the therapeutic agents into association with a carrier, which constitutes one or more accessory ingredients. Typically, the formulations are prepared by uniformly and intimately bringing the therapeutic agent into association with a liquid carrier, a finely divided solid carrier, or both, and then, if necessary, shaping the product into dosage forms of the desired formulation (e.g., wet or dry granulation, powder blends, efc, followed by tableting using conventional methods known in the art).
  • a carrier which constitutes one or more accessory ingredients.
  • the formulations are prepared by uniformly and intimately bringing the therapeutic agent into association with a liquid carrier, a finely divided solid carrier, or both, and then, if necessary, shaping the product into dosage forms of the desired formulation (e.g., wet or dry granulation, powder blends, efc,
  • any pharmaceutical compositions (and/or additional agents) described herein is formulated in accordance with routine procedures as a composition adapted for a mode of administration described herein.
  • Routes of administration include, for example: oral, intradermal, intramuscular, intraperitoneal, intravenous, subcutaneous, intranasal, epidural, sublingual, intranasal, intracerebral, intravaginal, transdermal, rectally, by inhalation, or topically.
  • Administration can be local or systemic.
  • the administering is effected orally.
  • the administration is by parenteral injection.
  • the mode of administration can be left to the discretion of the practitioner, and depends in-part upon the site of the medical condition. In most instances, administration results in the release of any agent described herein into the bloodstream.
  • compositions for oral delivery can be in the form of tablets, lozenges, aqueous or oily suspensions, granules, powders, emulsions, capsules, syrups, or elixirs, for example.
  • Orally administered compositions can comprise one or more agents, for example, sweetening agents such as fructose, aspartame or saccharin; flavoring agents such as peppermint, oil of wintergreen, or cherry; coloring agents; and preserving agents, to provide a pharmaceutically palatable preparation.
  • compositions can be coated to delay disintegration and absorption in the gastrointestinal tract thereby providing a sustained action over an extended period of time.
  • Selectively permeable membranes surrounding an osmotically active driving any CD8 binding agents described herein are also suitable for orally administered compositions. In these latter platforms, fluid from the environment surrounding the capsule is imbibed by the driving compound, which swells to displace the agent or agent composition through an aperture.
  • These delivery platforms can provide an essentially zero order delivery profile as opposed to the spiked profiles of immediate release formulations.
  • a time-delay material such as glycerol monostearate or glycerol stearate can also be useful.
  • Oral compositions can include standard excipients such as mannitol, lactose, starch, magnesium stearate, sodium saccharin, cellulose, and magnesium carbonate.
  • the excipients are of pharmaceutical grade.
  • Suspensions in addition to the active compounds, may contain suspending agents such as, for example, ethoxylated isostearyl alcohols, polyoxyethylene sorbitol and sorbitan esters, microcrystalline cellulose, aluminum metahydroxide, bentonite, agar-agar, tragacanth, efc, and mixtures thereof.
  • Dosage forms suitable for parenteral administration include, for example, solutions, suspensions, dispersions, emulsions, and the like. They may also be manufactured in the form of sterile solid compositions (e.g. lyophilized composition), which can be dissolved or suspended in sterile injectable medium immediately before use. They may contain, for example, suspending or dispersing agents known in the art.
  • Formulation components suitable for parenteral administration include a sterile diluent such as water for injection, saline solution, fixed oils, polyethylene glycols, glycerine, propylene glycol or other synthetic solvents; antibacterial agents such as benzyl alcohol or methyl paraben; antioxidants such as ascorbic acid or sodium bisulfite; chelating agents such as EDTA; buffers such as acetates, citrates or phosphates; and agents for the adjustment of tonicity such as sodium chloride or dextrose.
  • a sterile diluent such as water for injection, saline solution, fixed oils, polyethylene glycols, glycerine, propylene glycol or other synthetic solvents
  • antibacterial agents such as benzyl alcohol or methyl paraben
  • antioxidants such as ascorbic acid or sodium bisulfite
  • chelating agents such as EDTA
  • buffers such as acetates, citrates or phosphates
  • suitable carriers include physiological saline, bacteriostatic water, Cremophor ELTM (BASF, Parsippany, NJ) or phosphate buffered saline (PBS).
  • the carrier should be stable under the conditions of manufacture and storage, and should be preserved against microorganisms.
  • the carrier can be a solvent or dispersion medium containing, for example, water, ethanol, polyol (for example, glycerol, propylene glycol, and liquid polyetheylene glycol), and suitable mixtures thereof.
  • compositions provided herein can be made into aerosol formulations (i.e., "nebulized") to be administered via inhalation.
  • Aerosol formulations can be placed into pressurized acceptable propellants, such as dichlorodifluoromethane, propane, nitrogen, and the like.
  • compositions (and/or additional agents) described herein can be administered by controlled-release or sustained-release means or by delivery devices that are well known to those of ordinary skill in the art.
  • delivery devices include, but are not limited to, those described in U.S. Patent Nos. 3,845,770; 3,916,899; 3,536,809; 3,598,123; 4,008,719; 5,674,533; 5,059,595; 5,591 ,767; 5,120,548; 5,073,543; 5,639,476; 5,354,556; and 5,733,556, each of which is incorporated herein by reference in its entirety.
  • Such dosage forms can be useful for providing controlled- or sustained-release of one or more active ingredients using, for example, hydropropyl cellulose, hydropropylmethyl cellulose, polyvinylpyrrolidone, other polymer matrices, gels, permeable membranes, osmotic systems, multilayer coatings, microparticles, liposomes, microspheres, or a combination thereof to provide the desired release profile in varying proportions.
  • Suitable controlled- or sustained-release formulations known to those skilled in the art, including those described herein, can be readily selected for use with the active ingredients of the agents described herein.
  • the invention thus provides single unit dosage forms suitable for oral administration such as, but not limited to, tablets, capsules, gelcaps, and caplets that are adapted for controlled- or sustained-release.
  • Controlled- or sustained-release of an active ingredient can be stimulated by various conditions, including but not limited to, changes in pH, changes in temperature, stimulation by an appropriate wavelength of light, concentration or availability of enzymes, concentration or availability of water, or other physiological conditions or compounds.
  • a controlled-release system can be placed in proximity of the target area to be treated, thus requiring only a fraction of the systemic dose (see, e.g., Goodson, in Medical Applications of Controlled Release, supra, vol. 2, pp. 115-138 (1984)).
  • Other controlled-release systems discussed in the review by Langer, 1990, Science 249:1527-1533 may be used.
  • compositions preferably are sterile. Sterilization can be accomplished, for example, by filtration through sterile filtration membranes. Where the composition is lyophilized, filter sterilization can be conducted prior to or following lyophilization and reconstitution.
  • the actual dose of the CD8 binding agent to be administered according to the present invention will vary according to the particular dosage form, and the mode of administration. Many factors that may modify the action of the CD8 binding agent (e.g., body weight, gender, diet, time of administration, route of administration, rate of excretion, condition of the subject, drug combinations, genetic disposition and reaction sensitivities) can be taken into account by those skilled in the art. Administration can be carried out continuously or in one or more discrete doses within the maximum tolerated dose. Optimal administration rates for a given set of conditions can be ascertained by those skilled in the art using conventional dosage administration tests.
  • a suitable dosage of the CD8 binding agent is in a range of about 0.01 mg/kg to about 10 g/kg of body weight of the subject, about 0.01 mg/kg to about 1 g/kg of body weight of the subject, about 0.01 mg/kg to about 100 mg/kg of body weight of the subject, about 0.01 mg/kg to about 10 mg/kg of body weight of the subject, for example, about 0.01 mg/kg, about 0.02 mg/kg, about 0.03 mg/kg, about 0.04 mg/kg, about 0.05 mg/kg, about 0.06 mg/kg, about 0.07 mg/kg, about 0.08 mg/kg, about 0.09 mg/kg, about 0.1 mg/kg, about 0.2 mg/kg, about 0.3 mg/kg, about 0.4 mg/kg, about 0.5 mg/kg, about 0.6 mg/kg, about 0.7 mg/kg, about 0.8 mg/kg, about 0.9 mg/kg, about 1 mg/kg, about 1.1 mg/kg, about 1.2 mg/kg, about 1.3 mg/kg,
  • Individual doses of the CD8 binding agent can be administered in unit dosage forms containing, for example, from about 0.01 mg to about 100 g, from about 0.01 mg to about 75 g, from about 0.01 mg to about 50 g, from about 0.01 mg to about 25 g, about 0.01 mg to about 10 g, about 0.01 mg to about 7.5 g, about 0.01 mg to about 5 g, about 0.01 mg to about 2.5 g, about 0.01 mg to about 1 g, about 0.01 mg to about 100 mg, from about 0.1 mg to about 100 mg, from about 0.1 mg to about 90 mg, from about 0.1 mg to about 80 mg, from about 0.1 mg to about 70 mg, from about 0.1 mg to about 60 mg, from about 0.1 mg to about 50 mg, from about 0.1 mg to about 40 mg active ingredient, from about 0.1 mg to about 30 mg, from about 0.1 mg to about 20 mg, from about 0.1 mg to about 10 mg, from about 0.1 mg to about 5 mg, from about 0.1 mg to about 3 mg, from about 0.1 mg to
  • a unit dosage form can be about 0.01 mg, about 0.02 mg, about 0.03 mg, about 0.04 mg, about 0.05 mg, about 0.06 mg, about 0.07 mg, about 0.08 mg, about 0.09 mg, about 0.1 mg, about 0.2 mg, about 0.3 mg, about 0.4 mg, about 0.5 mg, about 0.6 mg, about 0.7 mg, about 0.8 mg, about 0.9 mg, about 1 mg, about 2 mg, about 3 mg, about 4 mg, about 5 mg, about 6 mg, about 7 mg, about 8 mg, about 9 mg about 10 mg, about 15 mg, about 20 mg, about 25 mg, about 30 mg, about 35 mg, about 40 mg, about 45 mg, about 50 mg, about 55 mg, about 60 mg, about 65 mg, about 70 mg, about 75 mg, about 80 mg, about 85 mg, about 90 mg, about 95 mg, about 100 mg, about 200 mg, about 500 mg, about 1 g, about 2.5 g, about 5 g, about 10 g, about 25 g, about 50 g, about 75 g, about 80 mg
  • the CD8 binding agent is administered at an amount of from about 0.01 mg to about 100 g daily, from about 0.01 mg to about 75 g daily, from about 0.01 mg to about 50 g daily, from about 0.01 mg to about 25 g daily, from about 0.01 mg to about 10 g daily, from about 0.01 mg to about 7.5 g daily, from about 0.01 mg to about 5 g daily, from about 0.01 mg to about 2.5 g daily, from about 0.01 mg to about 1 g daily, from about 0.01 mg to about 100 mg daily, from about 0.1 mg to about 100 mg daily, from about 0.1 mg to about 95 mg daily, from about 0.1 mg to about 90 mg daily, from about 0.1 mg to about 85 mg daily, from about 0.1 mg to about 80 mg daily, from about 0.1 mg to about 75 mg daily, from about 0.1 mg to about 70 mg daily, from about 0.1 mg to about 65 mg daily, from about 0.1 mg to about 60 mg daily, from about 0.1 mg to about 55 mg daily, from about 0.1 mg to about 50 mg daily
  • the CD8 binding agent is administered at a daily dose of about 0.01 mg, about 0.02 mg, about 0.03 mg, about 0.04 mg, about 0.05 mg, about 0.06 mg, about 0.07 mg, about 0.08 mg, about 0.09 mg, about 0.1 mg, about 0.2 mg, about 0.3 mg, about 0.4 mg, about 0.5 mg, about 0.6 mg, about 0.7 mg, about 0.8 mg, about 0.9 mg, about 1 mg, about 2 mg, about 3 mg, about 4 mg, about 5 mg, about 6 mg, about 7 mg, about 8 mg, about 9 mg about 10 mg, about 15 mg, about 20 mg, about 25 mg, about 30 mg, about 35 mg, about 40 mg, about 45 mg, about 50 mg, about 55 mg, about 60 mg, about 65 mg, about 70 mg, about 75 mg, about 80 mg, about 85 mg, about 90 mg, about 95 mg, about 100 mg, about 200 mg, about 500 mg, about 1 g, about 2.5 g, about 5 g, about 7.5 g, about 10 g, about 25
  • the pharmaceutical composition comprising the CD8 binding agent may be administered, for example, more than once daily (e.g., about two times, about three times, about four times, about five times, about six times, about seven times, about eight times, about nine times, or about ten times daily), about once per day, about every other day, about every third day, about once a week, about once every two weeks, about once every month, about once every two months, about once every three months, about once every six months, or about once every year.
  • more than once daily e.g., about two times, about three times, about four times, about five times, about six times, about seven times, about eight times, about nine times, or about ten times daily
  • about once per day about every other day, about every third day, about once a week, about once every two weeks, about once every month, about once every two months, about once every three months, about once every six months, or about once every year.
  • the pharmaceutical composition of the present invention is co-administered in conjunction with additional therapeutic agent(s). Co-administration can be simultaneous or sequential.
  • the additional therapeutic agent and the CD8 binding agent of the present invention are administered to a subject simultaneously.
  • the term "simultaneously" as used herein, means that the additional therapeutic agent and the CD8 binding agent are administered with a time separation of no more than about 60 minutes, such as no more than about 30 minutes, no more than about 20 minutes, no more than about 10 minutes, no more than about 5 minutes, or no more than about 1 minute.
  • Administration of the additional therapeutic agent and the CD8 binding agent can be by simultaneous administration of a single formulation (e.g., a formulation comprising the additional therapeutic agent and the CD8 binding agent) or of separate formulations (e.g., a first formulation including the additional therapeutic agent and a second formulation including the CD8 binding agent).
  • Co-administration does not require the therapeutic agents to be administered simultaneously, if the timing of their administration is such that the pharmacological activities of the additional therapeutic agent and the CD8 binding agent overlap in time, thereby exerting a combined therapeutic effect.
  • the additional therapeutic agent and the CD8 binding agent can be administered sequentially.
  • sequentially means that the additional therapeutic agent and the CD8 binding agent are administered with a time separation of more than about 60 minutes.
  • the time between the sequential administration of the additional therapeutic agent and the CD8 binding agent can be more than about 60 minutes, more than about 2 hours, more than about 5 hours, more than about 10 hours, more than about 1 day, more than about 2 days, more than about 3 days, more than about 1 week apart, more than about 2 weeks apart, or more than about one month apart.
  • the optimal administration times will depend on the rates of metabolism, excretion, and/or the pharmacodynamic activity of the additional therapeutic agent and the CD8 binding agent being administered. Either the additional therapeutic agent or the CD8 binding agent cell may be administered first.
  • Co-administration also does not require the therapeutic agents to be administered to the subject by the same route of administration. Rather, each therapeutic agent can be administered by any appropriate route, for example, parenterally or non-parenterally.
  • the CD8 binding agent described herein acts synergistically when co-administered with another therapeutic agent.
  • the CD8 binding agent and the additional therapeutic agent may be administered at doses that are lower than the doses employed when the agents are used in the context of monotherapy.
  • the present invention pertains to chemotherapeutic agents as additional therapeutic agents.
  • chemotherapeutic agents include, but are not limited to, alkylating agents such as thiotepa and CYTOXAN cyclosphosphamide; alkyl sulfonates such as busulfan, improsulfan and piposulfan; aziridines such as benzodopa, carboquone, meturedopa, and uredopa; ethylenimines and methylamelamines including altretamine, triethylenemelamine, trietylenephosphoramide, triethiylenethiophosphoramide and trimethylolomelamine; acetogenins (e.g., bullatacin and bullatacinone); a camptothecin (including the synthetic analogue topot
  • dynemicin including dynemicin A; bisphosphonates, such as clodronate; an esperamicin; as well as neocarzinostatin chromophore and related chromoprotein enediyne antibiotic chromophores), aclacinomysins, actinomycin, authramycin, azaserine, bleomycins, cactinomycin, carabicin, caminomycin, carzinophilin, chromomycinis, dactinomycin, daunorubicin, detorubicin, 6-diazo-5-oxo-L-norleucine, ADRIAMYCIN doxorubicin (including morpholino- doxorubicin, cyanomorpholino-doxorubicin, 2-pyrrolino-doxorubicin and deoxy doxorubicin), epirub
  • irinotecan Camptosar, CPT-11 (including the treatment regimen of irinotecan with 5-FU and leucovorin); topoisomerase inhibitor RFS 2000; difluoromethylornithine (DMFO); retinoids such as retinoic acid; capecitabine; combretastatin; leucovorin (LV); oxaliplatin, including the oxaliplatin treatment regimen (FOLFOX); lapatinib (Tykerb); inhibitors of PKC-a, Raf, H-Ras, EGFR (e.g., erlotinib (Tarceva)) and VEGF-A that reduce cell proliferation and pharmaceutically acceptable salts, acids or derivatives of any of the above.
  • the methods of treatment can further include the use
  • the present invention pertains to anti-infectives as additional therapeutic agents.
  • the anti-infective is an anti- viral agent including, but not limited to, Abacavir, Acyclovir, Adefovir, Amprenavir, Atazanavir, Cidofovir, Darunavir, Delavirdine, Didanosine, Docosanol, Efavirenz, Elvitegravir, Emtricitabine, Enfuvirtide, Etravirine, Famciclovir, and Foscarnet.
  • the anti-infective is an anti-bacterial agent including, but not limited to, cephalosporin antibiotics (cephalexin, cefuroxime, cefadroxil, cefazolin, cephalothin, cefaclor, cefamandole, cefoxitin, cefprozil, and ceftobiprole); fluoroquinolone antibiotics (cipro, Levaquin, floxin, tequin, avelox, and norflox); tetracycline antibiotics (tetracycline, minocycline, oxytetracycline, and doxycycline); penicillin antibiotics (amoxicillin, ampicillin, penicillin V, dicloxacillin, carbenicillin, vancomycin, and methicillin); monobactam antibiotics (aztreonam); and carbapenem antibiotics (ertapenem, doripenem, imipenem/cilastatin, and meropenem).
  • cephalosporin antibiotics ce
  • the anti-infectives include anti-malarial agents (e.g., chloroquine, quinine, mefloquine, primaquine, doxycycline, artemether/lumefantrine, atovaquone/proguanil and sulfadoxine/pyrimethamine), metronidazole, tinidazole, ivermectin, pyrantel pamoate, and albendazole.
  • anti-malarial agents e.g., chloroquine, quinine, mefloquine, primaquine, doxycycline, artemether/lumefantrine, atovaquone/proguanil and sulfadoxine/pyrimethamine
  • metronidazole e.g., chloroquine, quinine, mefloquine, primaquine, doxycycline, artemether/lumefantrine, atovaquone/proguanil and sulfa
  • the additional therapeutic agent is an immunosuppressive agent.
  • the immunosuppressive agent is an antiinflammatory agent such as a steroidal anti-inflammatory agent or a non-steroidal anti-inflammatory agent (NSAID).
  • NSAID non-steroidal anti-inflammatory agent
  • Steroids, particularly the adrenal corticosteroids and their synthetic analogues, are well known in the art.
  • NSAIDS that may be used in the present invention, include but are not limited to, salicylic acid, acetyl salicylic acid, methyl salicylate, glycol salicylate, salicylmides, benzyl-2,5-diacetoxybenzoic acid, ibuprofen, fulindac, naproxen, ketoprofen, etofenamate, phenylbutazone, and indomethacin.
  • the immunosupressive agent may be cytostatics such as alkylating agents, antimetabolites (e.g., azathioprine, methotrexate), cytotoxic antibiotics, antibodies (e.g., basiliximab, daclizumab, and muromonab), anti-immunophilins (e.g., cyclosporine, tacrolimus, sirolimus), inteferons, opioids, TNF binding proteins, mycophenolates, and small biological agents (e.g., fingolimod, myriocin). Additional anti-inflammatory agents are described, for example, in U.S. Patent No. 4,537,776, the entire contents of which is incorporated by reference herein.
  • the present invention relates to combination therapy with one or more immune- modulating agents, for example, without limitation, agents that modulate immune checkpoint.
  • the immune-modulating agent targets one or more of PD-1 , PD-L1 , and PD-L2.
  • the immune-modulating agent is PD-1 inhibitor.
  • the immune-modulating agent is an antibody specific for one or more of PD-1 , PD-L1 , and PD-L2.
  • the immune-modulating agent is an antibody such as, by way of non-limitation, nivolumab, (ONO-4538/BMS- 936558, MDX1106, OPDIVO, BRISTOL MYERS SQUIBB), pembrolizumab (KEYTRUDA, MERCK), pidilizumab (CT-011 , CURE TECH), MK-3475 (MERCK), BMS 936559 (BRISTOL MYERS SQUIBB), MPDL3280A (ROCHE).
  • the immune-modulating agent targets one or more of CD137 or CD137L.
  • the immune-modulating agent is an antibody specific for one or more of CD137 or CD137L.
  • the immune-modulating agent is an antibody such as, by way of non-limitation, urelumab (also known as BMS-663513 and anti-4-1 BB antibody).
  • the present CD8 binding agent is combined with urelumab (optionally with one or more of nivolumab, lirilumab, and urelumab) for the treatment of solid tumors and/or B-cell non-Hodgkins lymphoma and/or head and neck cancer and/or multiple myeloma.
  • the immune-modulating agent is an agent that targets one or more of CTLA-4, AP2M1 , CD80, CD86, SHP-2, and PPP2R5A.
  • the immune-modulating agent is an antibody specific for one or more of CTLA-4, AP2M1 , CD80, CD86, SHP-2, and PPP2R5A.
  • the immune-modulating agent is an antibody such as, by way of non-limitation, ipilimumab (MDX-010, MDX-101 , Yervoy, BMS) and/or tremelimumab (Pfizer).
  • the present CD8 binding agent is combined with ipilimumab (optionally with bavituximab) for the treatment of one or more of melanoma, prostate cancer, and lung cancer.
  • the immune-modulating agent targets CD20.
  • the immune-modulating agent is an antibody specific CD20.
  • the immune-modulating agent is an antibody such as, by way of non-limitation, Ofatumumab (GENMAB), obinutuzumab (GAZYVA), AME-133v (APPLIED MOLECULAR EVOLUTION), Ocrelizumab (GENENTECH), TRU-015 (TRUBION/EMERGENT), veltuzumab (IMMU-106).
  • the present invention relates to combination therapy with one or more chimeric agents described in WO 2013/10779, WO 2015/007536, WO 2015/007520, WO 2015/007542, and WO 2015/007903, the entire contents of which are hereby incorporated by reference in their entireties.
  • the CD8 binding agent described herein include derivatives that are modified, i.e., by the covalent attachment of any type of molecule to the composition such that covalent attachment does not prevent the activity of the composition.
  • derivatives include composition that have been modified by, inter alia, glycosylation, lipidation, acetylation, pegylation, phosphorylation, amidation, derivatization by known protecting/blocking groups, proteolytic cleavage, linkage to a cellular ligand or other protein, efc. Any of numerous chemical modifications can be carried out by known techniques, including, but not limited to specific chemical cleavage, acetylation, formylation, metabolic synthesis of tunicamycin, efc.
  • the CD8 binding agent described herein further comprise a cytotoxic agent, comprising, in illustrative embodiments, a toxin, a chemotherapeutic agent, a radioisotope, and an agent that causes apoptosis or cell death.
  • a cytotoxic agent comprising, in illustrative embodiments, a toxin, a chemotherapeutic agent, a radioisotope, and an agent that causes apoptosis or cell death.
  • agents may be conjugated to a composition described herein.
  • the CD8 binding agent described herein may thus be modified post-translationally to add effector moieties such as chemical linkers, detectable moieties such as for example fluorescent dyes, enzymes, substrates, bioluminescent materials, radioactive materials, and chemiluminescent moieties, or functional moieties such as for example streptavidin, avidin, biotin, a cytotoxin, a cytotoxic agent, and radioactive materials.
  • effector moieties such as chemical linkers, detectable moieties such as for example fluorescent dyes, enzymes, substrates, bioluminescent materials, radioactive materials, and chemiluminescent moieties, or functional moieties such as for example streptavidin, avidin, biotin, a cytotoxin, a cytotoxic agent, and radioactive materials.
  • Illustrative cytotoxic agents include, but are not limited to, methotrexate, aminopterin, 6-mercaptopurine, 6- thioguanine, cytarabine, 5-fluorouracil decarbazine; alkylating agents such as mechlorethamine, thioepa chlorambucil, melphalan, carmustine (BSNU), mitomycin C, lomustine (CCNU), 1-methylnitrosourea, cyclothosphamide, mechlorethamine, busulfan, dibromomannitol, streptozotocin, mitomycin C, cis- dichlorodiamine platinum (II) (DDP) cisplatin and carboplatin (paraplatin); anthracyclines include daunorubicin (formerly daunomycin), doxorubicin (adriamycin), detorubicin, carminomycin, idarubicin, epirubicin, mitoxantron
  • cytotoxic agents include paclitaxel (taxol), ricin, pseudomonas exotoxin, gemcitabine, cytochalasin B, gramicidin D, ethidium bromide, emetine, etoposide, tenoposide, colchicin, dihydroxy anthracin dione, 1- dehydrotestosterone, glucocorticoids, procaine, tetracaine, lidocaine, propranolol, puromycin, procarbazine, hydroxyurea, asparaginase, corticosteroids, mytotane (0,P'-(DDD)), interferons, and mixtures of these cytotoxic agents.
  • taxol taxol
  • ricin pseudomonas exotoxin
  • gemcitabine cytochalasin B
  • gramicidin D ethidium bromide
  • emetine emetine
  • etoposide tenoposide
  • cytotoxic agents include, but are not limited to, chemotherapeutic agents such as carboplatin, cisplatin, paclitaxel, gemcitabine, calicheamicin, doxorubicin, 5-fluorouracil, mitomycin C, actinomycin D, cyclophosphamide, vincristine, bleomycin, VEGF antagonists, EGFR antagonists, platins, taxols, irinotecan, 5- fluorouracil, gemcytabine, leucovorine, steroids, cyclophosphamide, melphalan, vinca alkaloids (e.g., vinblastine, vincristine, vindesine and vinorelbine), mustines, tyrosine kinase inhibitors, radiotherapy, sex hormone antagonists, selective androgen receptor modulators, selective estrogen receptor modulators, PDGF antagonists, TNF antagonists, IL-1 antagonists, interleukins (e.g.
  • IL-12 or IL-2 IL-12R antagonists
  • Toxin conjugated monoclonal antibodies tumor antigen specific monoclonal antibodies
  • Erbitux Avastin
  • Pertuzumab anti-CD20 antibodies
  • Rituxan ocrelizumab
  • ofatumumab DXL625, HERCEPTIN®, or any combination thereof.
  • Toxic enzymes from plants and bacteria such as ricin, diphtheria toxin and Pseudomonas toxin may be conjugated to the therapeutic agents (e.g. antibodies) to generate cell-type-specific-killing reagents (Youle, ef a/., Proc. Nat'l Acad. Sci.
  • cytotoxic agents include cytotoxic ribonucleases as described by Goldenberg in U.S. Pat. No. 6,653,104.
  • Embodiments of the invention also relate to radioimmunoconjugates where a radionuclide that emits alpha or beta particles is stably coupled to the CD8 binding agent, with or without the use of a complex-forming agent.
  • radionuclides include beta-emitters such as Phosphorus-32, Scandium-47, Copper-67, Gallium-67, Yttrium- 88, Yttrium-90, lodine-125, lodine-131 , Samarium-153, Lutetium-177, Rhenium-186 or Rhenium-188, and alpha- emitters such as Astatine-211 , Lead-212, Bismuth-212, Bismuth-213 or Actinium-225.
  • beta-emitters such as Phosphorus-32, Scandium-47, Copper-67, Gallium-67, Yttrium- 88, Yttrium-90, lodine-125, lodine-131 , Samarium-153, Lutetium-177, Rhenium-186 or Rhenium-188, and alpha- emitters such as Astatine-211 , Lead-212, Bismuth-212, Bismuth-213 or Actinium-225.
  • Illustrative detectable moieties further include, but are not limited to, horseradish peroxidase, acetylcholinesterase, alkaline phosphatase, beta-galactosidase and luciferase.
  • Further illustrative fluorescent materials include, but are not limited to, rhodamine, fluorescein, fluorescein isothiocyanate, umbelliferone, dichlorotriazinylamine, phycoerythrin and dansyl chloride.
  • Further illustrative chemiluminescent moieties include, but are not limited to, luminol.
  • Further illustrative bioluminescent materials include, but are not limited to, luciferin and aequorin.
  • Further illustrative radioactive materials include, but are not limited to, lodine-125, Carbon-14, Sulfur-35, Tritium and Phosphorus-32.
  • Methods and compositions described herein have application to treating various diseases and disorders, including, but not limited to cancer, infections, immune disorders, inflammatory diseases or conditions, and autoimmune diseases.
  • any of the present agents may be for use in the treating, or the manufacture of a medicament for treating, various diseases and disorders, including, but not limited to cancer, infections, immune disorders, inflammatory diseases or conditions, and autoimmune diseases.
  • the present invention relates to the treatment of, or a patient having cancer.
  • cancer refers to any uncontrolled growth of cells that may interfere with the normal functioning of the bodily organs and systems, and includes both primary and metastatic tumors.
  • Primary tumors or cancers that migrate from their original location and seed vital organs can eventually lead to the death of the subject through the functional deterioration of the affected organs.
  • a metastasis is a cancer cell or group of cancer cells, distinct from the primary tumor location, resulting from the dissemination of cancer cells from the primary tumor to other parts of the body. Metastases may eventually result in death of a subject.
  • cancers can include benign and malignant cancers, polyps, hyperplasia, as well as dormant tumors or micrometastases.
  • Illustrative cancers that may be treated include, but are not limited to, basal cell carcinoma, biliary tract cancer; bladder cancer; bone cancer; brain and central nervous system cancer; breast cancer; cancer of the peritoneum; cervical cancer; choriocarcinoma; colon and rectum cancer; connective tissue cancer; cancer of the digestive system; endometrial cancer; esophageal cancer; eye cancer; cancer of the head and neck; gastric cancer (including gastrointestinal cancer); glioblastoma; hepatic carcinoma; hepatoma; intra-epithelial neoplasm; kidney or renal cancer; larynx cancer; leukemia; liver cancer; lung cancer (e.g., small-cell lung cancer, non-small cell lung cancer, adenocarcinoma of the lung, and squamous carcinoma of the lung); melanoma;
  • Illustrative cancers that may be treated include, but are not limited to, carcinomas, e.g. various subtypes, including, for example, adenocarcinoma, basal cell carcinoma, squamous cell carcinoma, and transitional cell carcinoma), sarcomas (including, for example, bone and soft tissue), leukemias (including, for example, acute myeloid, acute lymphoblastic, chronic myeloid, chronic lymphocytic, and hairy cell), lymphomas and myelomas (including, for example, Hodgkin and non-Hodgkin lymphomas, light chain, non-secretory, MGUS, and plasmacytomas), and central nervous system cancers (including, for example, brain (e.g.
  • gliomas e.g. astrocytoma, oligodendroglioma, and ependymoma
  • meningioma e.g. astrocytoma, oligodendroglioma, and ependymoma
  • pituitary adenoma e.g. astrocytoma, oligodendroglioma, and ependymoma
  • spinal cord tumors e.g. meningiomas and neurofibroma
  • the present invention relates to the treatment of, or a patient having a microbial infection and/or chronic infection.
  • infections include, but are not limited to, HIV/AIDS, tuberculosis, osteomyelitis, hepatitis B, hepatitis C, Epstein-Barr virus or parvovirus, T cell leukemia virus, bacterial overgrowth syndrome, fungal or parasitic infections.
  • the present compositions are used to treat or prevent one or more inflammatory diseases or conditions, such as inflammation, acute inflammation, chronic inflammation, respiratory disease, atherosclerosis, restenosis, asthma, allergic rhinitis, atopic dermatitis, septic shock, rheumatoid arthritis, inflammatory bowel disease, inflammatory pelvic disease, pain, ocular inflammatory disease, celiac disease, Leigh Syndrome, Glycerol Kinase Deficiency, Familial eosinophilia (FE), autosomal recessive spastic ataxia, laryngeal inflammatory disease; Tuberculosis, Chronic cholecystitis, Bronchiectasis, Silicosis and other pneumoconioses.
  • inflammatory diseases or conditions such as inflammation, acute inflammation, chronic inflammation, respiratory disease, atherosclerosis, restenosis, asthma, allergic rhinitis, atopic dermatitis, septic shock, rheumatoid arthritis
  • the present compositions are used to treat or prevent one or more autoimmune diseases or conditions, such as multiple sclerosis, diabetes mellitus, lupus, celiac disease, Crohn's disease, ulcerative colitis, Guillain-Barre syndrome, scleroderms, Goodpasture's syndrome, Wegener's granulomatosis, autoimmune epilepsy, Rasmussen's encephalitis, Primary biliary sclerosis, Sclerosing cholangitis, Autoimmune hepatitis, Addison's disease, Hashimoto's thyroiditis, Fibromyalgia, Menier's syndrome; transplantation rejection (e.g., prevention of allograft rejection) pernicious anemia, rheumatoid arthritis, systemic lupus erythematosus, dermatomyositis, Sjogren's syndrome, lupus erythematosus, multiple sclerosis, myasthenia
  • kits for the administration of any CD8 binding agent described herein e.g. with or without additional therapeutic agents.
  • the kit is an assemblage of materials or components, including at least one of the inventive pharmaceutical compositions described herein.
  • the kit contains at least one of the pharmaceutical compositions described herein.
  • the kit is configured for the purpose of treating human subjects.
  • Instructions for use may be included in the kit.
  • Instructions for use typically include a tangible expression describing the technique to be employed in using the components of the kit to effect a desired therapeutic outcome, such as to treat cancer.
  • the kit also contains other useful components, such as, diluents, buffers, pharmaceutically acceptable carriers, syringes, catheters, applicators, pipetting or measuring tools, bandaging materials or other useful paraphernalia as will be readily recognized by those of skill in the art.
  • the materials and components assembled in the kit can be provided to the practitioner stored in any convenience and suitable ways that preserve their operability and utility.
  • the components can be provided at room, refrigerated or frozen temperatures.
  • the components are typically contained in suitable packaging materials.
  • the packaging material is constructed by well-known methods, preferably to provide a sterile, contaminant-free environment.
  • the packaging material may have an external label which indicates the contents and/or purpose of the kit and/or its components.
  • an “effective amount,” when used in connection with medical uses is an amount that is effective for providing a measurable treatment, prevention, or reduction in the rate of pathogenesis of a disease of interest.
  • something is "decreased" if a read-out of activity and/or effect is reduced by a significant amount, such as by at least about 10%, at least about 20%, at least about 30%, at least about 40%, at least about 50%, at least about 60%, at least about 70%, at least about 80%, at least about 90%, at least about 95%, at least about 97%, at least about 98%, or more, up to and including at least about 100%, in the presence of an agent or stimulus relative to the absence of such modulation.
  • activity is decreased and some downstream read-outs will decrease but others can increase.
  • activity is "increased” if a read-out of activity and/or effect is increased by a significant amount, for example by at least about 10%, at least about 20%, at least about 30%, at least about 40%, at least about 50%, at least about 60%, at least about 70%, at least about 80%, at least about 90%, at least about 95%, at least about 97%, at least about 98%, or more, up to and including at least about 100% or more, at least about 2-fold, at least about 3-fold, at least about 4-fold, at least about 5-fold, at least about 6-fold, at least about 7-fold, at least about 8-fold, at least about 9-fold, at least about 10-fold, at least about 50-fold, at least about 100-fold, in the presence of an agent or stimulus, relative to the absence of such agent or stimulus.
  • compositional percentages are by weight of the total composition, unless otherwise specified.
  • the word "include,” and its variants is intended to be non-limiting, such that recitation of items in a list is not to the exclusion of other like items that may also be useful in the compositions and methods of this technology.
  • the terms “can” and “may” and their variants are intended to be non- limiting, such that recitation that an embodiment can or may comprise certain elements or features does not exclude other embodiments of the present technology that do not contain those elements or features.
  • the words "preferred” and “preferably” refer to embodiments of the technology that afford certain benefits, under certain circumstances. However, other embodiments may also be preferred, under the same or other circumstances. Furthermore, the recitation of one or more preferred embodiments does not imply that other embodiments are not useful, and is not intended to exclude other embodiments from the scope of the technology.
  • compositions described herein needed for achieving a therapeutic effect may be determined empirically in accordance with conventional procedures for the particular purpose.
  • the therapeutic agents are given at a pharmacologically effective dose.
  • a “pharmacologically effective amount,” “pharmacologically effective dose,” “therapeutically effective amount,” or “effective amount” refers to an amount sufficient to produce the desired physiological effect or amount capable of achieving the desired result, particularly for treating the disorder or disease.
  • An effective amount as used herein would include an amount sufficient to, for example, delay the development of a symptom of the disorder or disease, alter the course of a symptom of the disorder or disease (e.g., slow the progression of a symptom of the disease), reduce or eliminate one or more symptoms or manifestations of the disorder or disease, and reverse a symptom of a disorder or disease.
  • Therapeutic benefit also includes halting or slowing the progression of the underlying disease or disorder, regardless of whether improvement is realized.
  • Effective amounts, toxicity, and therapeutic efficacy can be determined by standard pharmaceutical procedures in cell cultures or experimental animals, e.g., for determining the LD50 (the dose lethal to about 50% of the population) and the ED50 (the dose therapeutically effective in about 50% of the population).
  • the dosage can vary depending upon the dosage form employed and the route of administration utilized.
  • the dose ratio between toxic and therapeutic effects is the therapeutic index and can be expressed as the ratio LD50/ED50.
  • compositions and methods that exhibit large therapeutic indices are preferred.
  • a therapeutically effective dose can be estimated initially from in vitro assays, including, for example, cell culture assays.
  • a dose can be formulated in animal models to achieve a circulating plasma concentration range that includes the IC50 as determined in cell culture, or in an appropriate animal model.
  • Levels of the described compositions in plasma can be measured, for example, by high performance liquid chromatography.
  • the effects of any particular dosage can be monitored by a suitable bioassay. The dosage can be determined by a physician and adjusted, as necessary, to suit observed effects of the treatment.
  • the effect will result in a quantifiable change of at least about 10%, at least about 20%, at least about 30%, at least about 50%, at least about 70%, or at least about 90%. In some embodiments, the effect will result in a quantifiable change of about 10%, about 20%, about 30%, about 50%, about 70%, or even about 90% or more.
  • Therapeutic benefit also includes halting or slowing the progression of the underlying disease or disorder, regardless of whether improvement is realized.
  • compositions for treating the diseases or disorders described herein are equally applicable to use of a composition for treating the diseases or disorders described herein and/or compositions for use and/or uses in the manufacture of a medicaments for treating the diseases or disorders described herein.
  • AcTaferon is occasionally used herein to reference an interferon-based chimera.
  • mutations to IFN are relative to human IFN-a2 - SEQ ID NO:25.
  • the Q124R mutant is representative of an attenuated human IFN alpha 2 mutant that can be assayed in vivo in a murine model.
  • Q124R is a human IFN mutation that is suitable for use in the mouse (i.e. it is a human mutant IFN that functions in mouse). See Nat. Comm. 2014;5:3016. doi: 10.1038/ncomms4016, the entire contents of which are hereby incorporated by reference.
  • the R33A/E120R mutant is representative of human IFN alpha 2 mutant that is non-functional (and is used as a control)
  • Anti-BcM O VHH is used in these Examples as a control (targeting an irrelevant antigen, i.e. "untargeted”).
  • a VHH library was subject to 2 consecutive rounds of panning (in solution), performed on stably transfected CHO-K1 cells expressing mouse CD8.
  • the enrichment for antigen-specific phages was assessed after each round of panning by comparing the number of phagemid particles eluted from transfected cells (output) with the number of phagemid particles used for panning (input).
  • the phage output increased about 10 2 -fold in the 2 nd round, as compared to the output from the 1 st round.
  • the input phage was always about 5 ⁇ 10 11 and the output from first round was about 10 9 phage particles.
  • VHH genes were redoned from pHEN4 to pHEN6c vectors. Specifically, the VHH gene was amplified by PCR using recombinant pHEN4 harboring the VHH gene as template and primers A6E and 38. Primers A6E and 38 were frameworkl and framework4 primers, respectively. The primer sequences were as follows:
  • Primer A6E (5' GAT GTG CAG CTG CAG GAG TCT GGR* GGA GG 3') (SEQ ID NO:228).
  • Primer 38 (5' GGA CTA GTG CGG CCG CTG GAG ACG GTG ACC TGG GT 3') (SEQ ID NO:229).
  • the amplification protocol included about 30 cycles of PCR, each cycle included 30 seconds at 94°C, 30 seconds at 55°C and 45 seconds at 72°C, followed by 10 minutes extension at 72°C at the end of PCR. A fragment of about 400 bp was amplified.
  • the PCR product was purified (e.g. by Qiaquick PCR purification kit from Qiagen) and digested overnight with Pstl.
  • the purified PCR product was then digested with BstEII overnight (or with Eco91 l from Fermentas).
  • the temperature used for digestion varied. For example, digestion with BstEII was done at 50°C or 60°C depending on the supplier of the enzyme.
  • the PCR product was purified.
  • the pHEN6c vector was digested with Pstl for 3 hours, purified as described above, and then digested with BstEII for 2 to 3 hours. Alternatively, digestion was carried out using Eco91 l from Fermentas.
  • the digested vector was ran on 1 % agarose gel, with the vector band excised out of the gel and purified (e.g. by Qiaquick gel extraction kit from Qiagen).
  • the PCR fragment was subsequently ligated to the vector.
  • Electrocompetent WK6 cells were transformed with the ligation reaction, and transformants were selected using LB/agar/ampicillin (100 ⁇ g/ml)/glucose (1%) plates.
  • Positive clones were screened by PCR using universal reverse and universal forward primers. A fragment of about 550 bp was amplified, if the insert was present. To verify the identity of the clones, at least 2 clones per each VHH were sequenced using universal reverse primers. Antigen binding capacity was retested by ELISA or any other appropriate assay.
  • VHH gene cloned in pHEN6c vector was generated which contained PelB signal sequence at the N-terminus and Hise-tail at the C-terminus.
  • the PelB leader sequence directed the VHH to the periplasmic space of the E.coli, and the His-tag was used for the purification and detection of VHH (e.g. in ELISA, Western Blot, efc).
  • VHHs were expressed and purification. Specifically, on day 1 , 10-20 ml of LB + ampicillin (100 g/ml) + glucose (1%) were innoculated with a freshly transformed WK6 colony. This pre-culture was incubated at 37°C overnight with shaking at 200-250 rpm. On day 2, a TB medium was used for expressing the VHHs. The TB medium included, per liter: 2.3 g KH 2 P0 4 , 16.4 g K 2 HP0 4 .3H 2 0, 12 g Tryptone (Duchefa Biochemie), 24 g Yeast (Duchefa Biochemie), and 4 ml 100% glycerol (Duchefa Biochemie).
  • a baffled shaker flask of 1 liter was filled with 330 ml TB and autoclaved.
  • KH 2 P0 4 and K 2 HP0 4 .3H 2 0 were not autoclaved. Instead, KH 2 P0 4 and K 2 HP0 4 .3H 2 0 were prepared, filter sterilized, and then added to the rest of the medium that was already autoclaved.
  • About 1 ml of the pre-culture was added to 330 ml of TB supplemented with 100 g/ml Ampicillin, 2 mM MgCI 2 and 0.1% glucose and subsequently grew at 37°C with shaking (200-250 rpm) until an ODwo of 0.6-0.9 was reached.
  • IPTG final concentration of 1 mM
  • the culture was incubated at 28°C with shaking overnight (about 16-18 hours).
  • the ODwo after overnight induction was usually between 25 and 30.
  • At least 1 liter of culture (3 bottles) per clone was prepared with an average yield of between 1 and 15 mg/l.
  • VHHs Extraction of the VHHs from the periplasm of E. coli was carried out on day 3.
  • the overnight induced cultures were centrifuged for 8 minutes at 8000 rpm.
  • the cell pellets from 1 liter culture were resuspended in 12 ml TES by pipetting up and down and shaken for 1 hour on ice.
  • Per each 12 ml TES used about 18 ml TES/4 were added and incubated on ice for an additional hour with shaking followed by centrifugation for 30 minutes at 8000 rpm at 4°C.
  • the supernatant which contained proteins extracted from the periplasmic spaced was transferred to fresh falcon tubes.
  • VHHs were subsequently purified by IMAC which utilized the following solution: HIS-select (SIGMA), PBS, and 50 mM NaAcetate pH 4.6.
  • SIGMA HIS-select
  • PBS PBS
  • His-select was equilibrated with PBS. Specifically, per periplasmic extract derived from 1 liter culture, 1 ml of Resin (about 2 ml His-select solution) was added to a 50 ml falcon tube. PBS was also added to final volume of 50 ml and mixed. Centrifugation was carried out at 2000 rpm for 2 minutes, and the supernatant was discarded. The resin was washed with PBS twice as described above. The periplasmic extract was added to the resin, incubated for 30 minutes to 1 hour at room temperature with gentle shaking. The samples were loaded on PD-10 columns with a filter at the bottom (GE healthcare, cat. No.
  • VHHs The amount of protein was estimated by OD280 measurement of eluted sample. Extinction coefficient of each clone was determined by protParam tool under primary structure analysis at the Expasy proteomics server. Further purification of VHHs could be achieved by different methods. For example, the samples could be concentrated (Vivaspin 5000 MW cutoff, Vivascience) by centrifuging at 2000 rpm at 4°C until an appropriate volume for loading on a Superdex 75 16/60 was obtained (max. 4 ml). The concentrated sample was loaded on a Superdex 75 16/60 column equilibrated with PBS. Peak fractions were pooled, and OD280 measurements were performed for quantification. In general, VHHs eluted after 85-95 minutes when run at 1 ml/min. Aliquots of concentrated VHH samples were stored at -20°C at a concentration of about 1 mg/ml.
  • VHHs were tested for binding to CD8. Specifically, mouse splenocytes were stained with anti-CD8 VHHs at 1 ug/ml and anti-His FITC mAb. Out of 31 positive VHHs, six were stained positive on splenocytes.
  • Figure 1, panels A-B show binding of the six VHHs to CHO cells transfected with CD8a or to splenocytes. In all experiments, the six selected VHHs were labeled as follows:
  • OT-I Rag-/- T cells were cultured for 48 hours in a 50:1 ratio with unloaded or OVA peptide (SIINFEKL, SEQ ID NO:232)-loaded dendritic cells.
  • Co-cultures were supplemented with 50 ng of the mCD8 VHHs, an irrelevant VHH or a monoclonal Ab known to block TCR activation (CT-CD8).
  • OVA-induced T cell activation was determined by measuring CFSE dilution and CD25 expression.
  • Co-cultures were performed in triplicate, cells were pooled for flow cytometric analysis. Results are shown in Figure 2.
  • none of the selected VHHs affected CTL activation, in stark contrast to CT-mAB, a monoclonal antibody known to interfere with the antigen presentation process.
  • the VHH gene cloned in pMECS vector contained PelB signal sequence at the N-terminus and HA tag and His6 tag at the C-terminus (PelB Ieader-VHH-HA-His6).
  • the PelB leader sequence directed the VHH to the periplasmic space of the E.coli, and the HA and Hise tags was used for the purification and detection of VHH [e.g. in ELISA, Western Blot, efc).
  • the Hise tag was followed by an amber stop codon (TAG) and this amber stop codon was followed by gene III of M13 phage.
  • TAG amber stop codon
  • suppressor E. coli strains e.g. TG1
  • the amber stop codon was read as glutamine and therefore the VHH was expressed as fusion protein with protein III of the phage which allowed the display of the VHH on the phage coat for panning.
  • the efficiency of suppression is not 100% and therefore the expression of VHHs in suppressor strains led to two different types of VHH molecules, fused to protein III and without protein III).
  • non-suppressor E. coli strains e. g., WK6
  • the amber stop codon was read as stop codon and therefore the resulting VHH was not fused to protein III.
  • pMECS In order to express and purify VHHs cloned in pMECS vector, pMECS was prepared containing the gene of the VHH of interest, and the plasmid was transformed into a non-suppressor strain (e.g., WK6).
  • the VHH of the resulting clone was sequenced using the MP057 primer (5'-TTATGCTTCCGGCTCGTATG-3') (SEQ ID NO:233) to verify the identity of the clone.
  • Antigen binding capacity was retested by ELISA or any other appropriate assay.
  • the non-suppressor strain (e.g., WK6) containing the recombinant pMECS vector with the VHH gene was used for the expression and purification of the VHH.
  • the Hise tag was cleaved off upon storage of the VHH. Accordingly, the VHH gene was recloned from pMECS into pHEN6c vector, if the His6 tag was to be used for detection, etc. Specifically, the VHH gene was amplified by PCR using recombinant pMECS harboring the VHH gene as template and primers A6E and PMCF. Primers A6E and PMCF were frameworki and framework4 primers, respectively. The primer sequences were as follows:
  • Primer A6E (5' GAT GTG CAG CTG CAG GAG TCT GGR* GGA GG 3') (SEQ ID NO:228).
  • Primer PMCF (5' CTA GTG CGG CCG CTG AGG AGA CGG TGA CCT GGG T 3') (SEQ ID NO:234).
  • the amplification protocol included about 30 cycles of PCR, each cycle included 30 seconds at 94°C, 30 seconds at 55°C and 45 seconds at 72°C, followed by 10 minutes extension at 72°C at the end of PCR. A fragment of about 400 bp was amplified.
  • the PCR product was purified (e.g. by Qiaquick PCR purification kit from Qiagen) and digested overnight with Pstl.
  • the purified PCR product was digested with BstEII overnight (or with Eco91 l from Fermentas).
  • the temperature used for digestion varied. For example, digestion with BstEII was done at 50°C or 60°C depending on the supplier of the enzyme.
  • the PCR product was purified.
  • the pHEN6c vector was digested with Pstl for 3 hours, purified as described above, and then digested with BstEII for 2 to 3 hours. Alternatively, digestion was carried out using Eco91 l from Fermentas.
  • the digested vector was ran on 1 % agarose gel, with the vector band excised out of the gel and purified (e.g. by Qiaquick gel extraction kit from Qiagen).
  • the PCR fragment was subsequently ligated to the vector.
  • Electrocompetent WK6 cells were transformed with the ligation reaction, and transformants were selected using LB/agar/ampicillin (100 ⁇ g/ml)/glucose (1-2%) plates.
  • Positive clones were screened by PCR using universal reverse and universal forward primers. A fragment of about 550 bp was amplified, if the insert was present. To verify the identity of the clones, at least 2 clones per each VHH were sequenced using universal reverse primers. Antigen binding capacity was retested by ELISA or any other appropriate assay.
  • VHH gene cloned in pHEN6c vector was generated which contained PelB signal sequence at the N-terminus and Hise-tail at the C-terminus.
  • the PelB leader sequence directed the VHH to the periplasmic space of the E.coli, and the His-tag was used for the purification and detection of VHH (e.g. in ELISA, Western Blot, efc).
  • VHHs were expressed and purification. Specifically, on day 1 , 10-20 ml of LB + ampicillin (100 g/ml) + glucose (1 %) were innoculated with a freshly transformed WK6 colony. This pre-culture was incubated at 37°C overnight with shaking at 200-250 rpm. On day 2, a TB medium was used for expressing the VHHs. The TB medium included, per liter: 2.3 g KH 2 P0 4 , 16.4 g K 2 HP0 4 .3H 2 0, 12 g Tryptone (Duchefa Biochemie), 24 g Yeast (Duchefa Biochemie), and 4 ml 100% glycerol (Duchefa Biochemie)
  • a baffled shaker flask of 1 liter was filled with 330 ml TB and autoclaved.
  • KH 2 P0 4 and K 2 HP0 4 .3H 2 0 were not autoclaved. Instead, KH 2 P0 4 and K 2 HP0 4 .3H 2 0 were prepared, filter sterilized, and then added to the rest of the medium that was already autoclaved.
  • About 1 ml of the pre-culture was added to 330 ml of TB supplemented with 100 g/ml Ampicillin, 2 mM MgCI 2 and 0.1 % glucose and subsequently grew at 37°C with shaking (200-250 rpm) until an ODwo of 0.6-0.9 was reached.
  • IPTG final concentration of 1 mM
  • the culture was incubated at 28°C with shaking overnight (about 16-18 hours).
  • the ODwo after overnight induction was usually between 25 and 30.
  • At least 1 liter of culture (3 bottles) per clone was prepared with an average yield of between 1 and 15 mg/l.
  • VHHs Extraction of the VHHs from the periplasm of E. coli was carried out on day 3.
  • the overnight induced cultures were centrifuged for 8 minutes at 8000 rpm.
  • the cell pellets from 1 liter culture were resuspended in 12 ml TES by pipetting up and down and shaken for 1 hour on ice.
  • Per each 12 ml TES used about 18 ml TES/4 were added and incubated on ice for an additional hour with shaking followed by centrifuge for 30 minutes at 8000 rpm at 4°C.
  • the supernatant which contained proteins extracted from the periplasmic spaced was transferred to fresh falcon tubes.
  • VHHs were subsequently purified by IMAC which utilized the following solution: HIS-select (SIGMA), PBS, and 50 mM NaAcetate pH 4.6.
  • SIGMA HIS-select
  • PBS PBS
  • His-select was equilibrated with PBS. Specifically, per periplasmic extract derived from 1 liter culture, 1 ml of Resin (about 2 ml His-select solution) was added to a 50 ml falcon tube. PBS was also added to final volume of 50 ml and mixed. Centrifugation was carried out at 2000 rpm for 2 minutes, and the supernatant was discarded. The resin was washed with PBS twice as described above. The periplasmic extract was added to the resin, incubated for 30 minutes to 1 hour at room temperature with gentle shaking. The samples were loaded on PD-10 columns with a filter at the bottom (GE healthcare, cat. No.
  • VHHs The amount of protein was estimated by OD280 measurement of eluted sample. Extinction coefficient of each clone was determined by protParam tool under primary structure analysis at the Expasy proteomics server. Further purification of VHHs could be achieved by different methods. For example, the samples could be concentrated (Vivaspin 5000 MW cutoff, Vivascience) by centrifuging at 2000 rpm at 4°C until an appropriate volume for loading on a Superdex 75 16/60 was obtained (max. 4 ml). The concentrated sample was loaded on a Superdex 75 16/60 column equilibrated with PBS. Peak fractions were pooled, and OD280 measurements were performed for quantification. In general, VHHs eluted after 85-95 minutes when run at 1 ml/min. Aliquots of concentrated VHH samples were stored at -20°C at a concentration of about 1 mg/ml.
  • VHHs comprising antigen recognition domains against human CD8 were constructed.
  • the VHHs were named R3HCD27, R3HCD129, and R2HCD26.
  • the amino acid sequences of the VHHs are as follows:
  • R3HCD129 (SEQ ID NO:20)
  • R2HCD26 (SEQ ID NO:21)
  • HEK293-T cells were transfected with a human CD8a expression plasmid and stained with the three His-tagged VHHs (i.e., R2HCD26, R3HCD27, and R3HCD129) at 2 glm ⁇ , followed by staining with an anti-His Fitc conjugated antibody. Cellular fluorescence was detected via flow cytometry. Results as shown in Figure 5 show that the VHHs bound to CD8.
  • PBMCs peripheral blood mononuclear cells
  • PBMCs peripheral blood mononuclear cells
  • Results indicate that at the 10 nM and 100 nM concentrations, all three VHHs had higher MFI than the control antibody (suggesting specific binding to CD8) with the R2HCD26 antibody showing the highest MFI.
  • Panel C of Figure 6 shows that a significant portion of CD3-antigen positive cells in the PBMC also stained positive for CD8.
  • Figure 7 shows the anti-tumor activities of a murine bispecific chimera ("CD8-Q124R-PD-L1") analyzed using the B16 melanoma model.
  • the bi-specific (anti-murine CD8 and anti-murine PD-L1) fusion to modified human IFN alpha (Q124R) provided better anti-tumor activity as compared to a fusion of anti-CD8 to modified human IFN alpha (Q124R).
  • Figure 8 shows a 4T1 mammary tumor model study in which a bi-specific (anti-murine CD8 and anti-murine PD- L1) fusion to modified human IFN alpha (Q124R) provided better anti-tumor activity as compared to the coadministration of a fusion of anti-CD8 to modified human IFN alpha (Q124R) and a fusion of anti-PD-L1 to modified human IFN alpha (Q124R) or the co-administration of a fusion of anti-CD8 to modified human IFN alpha (Q124R) and an anti-PD-L1 VHH.
  • a bi-specific (anti-murine CD8 and anti-murine PD- L1) fusion to modified human IFN alpha Q124R
  • Q124R bi-specific (anti-murine CD8 and anti-murine PD- L1) fusion to modified human IFN alpha
  • Figure 9 shows in the 4T1 mammary tumor model, the use of the bi-specific (anti-murine CD8 and anti-murine PD-L1) fusion to modified human IFN alpha (Q124R) with doxorubicin resulted in a curative effect in 3 out of 6 mice (i.e., the mice were completely tumor free).
  • PBMCs peripheral blood mononuclear cells
  • a chimera of anti-human CD8 VHH/human IFN R149A i.e., pmTW-SlgK-hCD8_R2HCD26 (SEQ ID NO:21)- (GGS) 20 -hlFNa2_R149A-GGS-(His) 9 construct
  • anti-human CD8 VHH/human IFN R33A/E120A i.e., pmTW- SlgK-hCD8_R2HCD26 (SEQ ID NO:21)-(GGS) 2 o-hlFNa2_R33A/E120A-GGS-(His) 9 construct
  • Cells were grown to a density of 0.6x10 6 cells per ml in Freestyle medium and transfected with 25K PEI (polyethylenimine) according to standard protocols. Three days after transfection, fresh medium was added to the cultures and cells were grown for two or three additional days. Medium was harvested, cells removed by centrifugation and filter-sterilized. Recombinant proteins were purified using Ni Excel resin (GE Healthcare) according to the manufacturer's instructions and imidazole removed from the samples with PD10 columns (GE Healthcare).
  • PEI polyethylenimine
  • PBMCs from buffy coats of healthy donors were isolated using density gradient centrifugation with Ficoll-Paque (GE Healthcare). Cells were washed twice with FACS buffer (2% FBS, 1 mM EDTA in PBS) and stained with anti-human CD8 APC (clone RPE-T8; BD Pharmingen) for 20 minutes at 4°C. After two washes, cells were stimulated with a serial dilution of CD8-targeting chimeras for 15 minutes at 37°C.
  • Isolated PBMCs were stimulated with a serial dilution of CD8-targeting chimeras (anti-human CD8 VHH/human IFN R149A, anti-human CD8 VHH/human IFN R33A/E120A, or anti-BclM O VHH/human IFN R149A) and stained for CD8 (APC) and pSTATI (PE).
  • CD8-targeting chimeras anti-human CD8 VHH/human IFN R149A, anti-human CD8 VHH/human IFN R33A/E120A, or anti-BclM O VHH/human IFN R149A
  • T cell receptor as a multicomponent signalling machine: CD4/CD8 coreceptors and CD45 in T cell activation. Annual Review of Immunology 10, 645-74.
  • the human CD8 coreceptor effects cytotoxic T cell activation and antigen sensitivity primarily by mediating complete phosphorylation of the T cell receptor zeta chain. 77?e Journal of Biological Chemistry 276, 32786-92.
  • CD4 and CD8 T cell surface antigens are associated with the internal membrane tyrosine-protein kinase p56lck. Cell 55, 301-8.
  • T cell receptor and coreceptor CD8 alphaalpha bind peptide-MHC independently and with distinct kinetics. Immunity ⁇ 10, 219-25.
  • CD4 and CD8 modulators of T-cell receptor recognition of antigen and of immune responses? Current Opinion in Immunology 10, 82-7.

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