ATE276359T1 - Verfahren zur herstellung von antikörperfragmenten - Google Patents

Verfahren zur herstellung von antikörperfragmenten

Info

Publication number
ATE276359T1
ATE276359T1 AT00901571T AT00901571T ATE276359T1 AT E276359 T1 ATE276359 T1 AT E276359T1 AT 00901571 T AT00901571 T AT 00901571T AT 00901571 T AT00901571 T AT 00901571T AT E276359 T1 ATE276359 T1 AT E276359T1
Authority
AT
Austria
Prior art keywords
library
nucleic acid
antibody fragments
repertoire
naïve
Prior art date
Application number
AT00901571T
Other languages
English (en)
Inventor
Leon Gerardus Joseph Frenken
Der Logt Cornelis Paul Eri Van
Original Assignee
Unilever Nv
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=8241190&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=ATE276359(T1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Unilever Nv filed Critical Unilever Nv
Application granted granted Critical
Publication of ATE276359T1 publication Critical patent/ATE276359T1/de

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Classifications

    • CCHEMISTRY; METALLURGY
    • C40COMBINATORIAL TECHNOLOGY
    • C40BCOMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00Libraries per se, e.g. arrays, mixtures
    • C40B40/02Libraries contained in or displayed by microorganisms, e.g. bacteria or animal cells; Libraries contained in or displayed by vectors, e.g. plasmids; Libraries containing only microorganisms or vectors
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K16/00Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034Isolating an individual clone by screening libraries
    • C12N15/1037Screening libraries presented on the surface of microorganisms, e.g. phage display, E. coli display

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Immunology (AREA)
  • Virology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
AT00901571T 1999-01-19 2000-01-13 Verfahren zur herstellung von antikörperfragmenten ATE276359T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP99300351 1999-01-19
PCT/EP2000/000296 WO2000043507A1 (en) 1999-01-19 2000-01-13 Method for producing antibody fragments

Publications (1)

Publication Number Publication Date
ATE276359T1 true ATE276359T1 (de) 2004-10-15

Family

ID=8241190

Family Applications (1)

Application Number Title Priority Date Filing Date
AT00901571T ATE276359T1 (de) 1999-01-19 2000-01-13 Verfahren zur herstellung von antikörperfragmenten

Country Status (7)

Country Link
US (2) US6399763B1 (de)
EP (1) EP1144616B2 (de)
AT (1) ATE276359T1 (de)
AU (1) AU2291700A (de)
DE (1) DE60013767T3 (de)
DK (1) DK1144616T4 (de)
WO (1) WO2000043507A1 (de)

Families Citing this family (249)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1999037681A2 (en) * 1998-01-26 1999-07-29 Unilever Plc Method for producing antibody fragments
US7297478B1 (en) 2000-09-22 2007-11-20 Large Scale Biology Corporation Creation of variable length and sequence linker regions for dual-domain or multi-domain molecules
CA2441903C (en) 2000-05-26 2012-07-31 National Research Council Of Canada Single-domain brain-targeting antibody fragments derived from llama antibodies
US20030190598A1 (en) * 2000-05-26 2003-10-09 Jasmid Tanha Single-domain antigen-binding antibody fragments derived from llama antibodies
ATE352040T1 (de) 2000-11-17 2007-02-15 Univ Rochester In-vitro verfahren zur herstellung und identifizierung von immunglobulin moleküle in eukaryotischen zellen
WO2003025020A1 (en) * 2001-09-13 2003-03-27 Institute For Antibodies Co., Ltd. Method of constructing camel antibody library
EP1318195A1 (de) 2001-12-10 2003-06-11 CatchMabs B.V. Ein Struktur für die Präsentation erwünschter Peptidsequenzen
ES2528631T3 (es) 2003-04-08 2015-02-11 Progenics Pharmaceuticals, Inc. Formulaciones farmacéuticas que contienen metilnaltrexona
CN1214118C (zh) * 2003-07-18 2005-08-10 文剑 Dna抗体及其应用
US20050053989A1 (en) 2003-08-27 2005-03-10 Gil Sharon Libraries of recombinant chimeric proteins
US7642341B2 (en) 2003-12-18 2010-01-05 Merck Serono S.A. Angiogenesis inhibiting molecules, their selection, production and their use in the treatment of cancer
AU2005222788A1 (en) * 2004-02-27 2005-09-29 President And Fellows Of Harvard College Polynucleotide synthesis
US7785903B2 (en) 2004-04-09 2010-08-31 Genentech, Inc. Variable domain library and uses
US20050272917A1 (en) * 2004-05-14 2005-12-08 Hematech, Llc Methods for immunoglobulin purification
US20070298443A1 (en) * 2004-06-04 2007-12-27 Yiyou Chen Screening Method Using Antibody Heavy Chains
KR20070084069A (ko) 2004-10-08 2007-08-24 도만티스 리미티드 Tnfr1에 대한 단일 도메인 항체 및 이의 사용 방법
AU2006205900B8 (en) 2005-01-14 2012-04-05 Ablynx N.V. Methods and assays for distinguishing between different forms of diseases and disorders characterized by thrombocytopenia and/or by spontaneous interaction between Von Willebrand Factor (vWF) and platelets
EP2949668B1 (de) 2005-05-18 2019-08-14 Ablynx N.V. Verbesserte nanokörper-tm gegen tumornekrosefaktor alpha
BRPI0609797B8 (pt) 2005-05-20 2021-05-25 Ablynx Nv nanocorpos melhorados para o tratamento de desordens mediadas por agregação
US20090047300A1 (en) * 2005-09-27 2009-02-19 Abedelnasser Abulrob Blood-brain barrier epitopes and uses thereof
AR060871A1 (es) * 2006-05-09 2008-07-16 Genentech Inc Union de polipeptidos con supercontigos optimizados
WO2007137616A1 (en) * 2006-05-30 2007-12-06 Millegen Highly diversified antibody libraries
TWI489984B (zh) 2006-08-04 2015-07-01 Wyeth Corp 用於非經腸道傳輸化合物之配方及其用途
US8007797B2 (en) * 2006-09-28 2011-08-30 Merck Serono S.A. Junctional adhesion molecule-C (JAM-C) binding compounds and methods of their use
WO2008044928A1 (en) 2006-10-10 2008-04-17 Academisch Ziekenhuis Bij De Universiteit Van Amsterdam Complement inhibition for improved nerve regeneration
GB0621513D0 (en) 2006-10-30 2006-12-06 Domantis Ltd Novel polypeptides and uses thereof
JP5105468B2 (ja) * 2006-12-05 2012-12-26 独立行政法人産業技術総合研究所 細胞内で機能する抗体の創製および細胞表現型を制御するタンパク質の同定
AU2007336242B2 (en) 2006-12-19 2012-08-30 Ablynx N.V. Amino acid sequences directed against GPCRs and polypeptides comprising the same for the treatment of GPCR-related diseases and disorders
EP2514767A1 (de) 2006-12-19 2012-10-24 Ablynx N.V. Gegen Metalloproteinase der ADAM-Familie gerichtete Aminosäuren und Polypeptide damit zur Behandlung von Erkrankungen und Störungen im Zusammenhang mit ADAM
PL2308514T3 (pl) 2007-03-23 2013-11-29 To Bbb Holding B V Koniugaty do ukierunkowanego dostarczania leku poprzez barierę krew-mózg
MX2009010550A (es) 2007-03-29 2009-12-14 Progenics Pharm Inc Formas de cristal de bromuro de (r)-n-metilnaltrexona y uso de las mismas.
AU2008270274B2 (en) 2007-07-03 2012-06-28 Ablynx N.V. Providing improved immunoglobulin sequences by mutating CDR and/or FR positions
AU2008328779B2 (en) 2007-11-27 2014-06-05 Ablynx N.V. Amino acid sequences directed against HER2 and polypeptides comprising the same for the treatment of cancers and/or tumors
KR20150080038A (ko) 2007-11-30 2015-07-08 애브비 바이오테크놀로지 리미티드 단백질 제형 및 이의 제조방법
US8883146B2 (en) 2007-11-30 2014-11-11 Abbvie Inc. Protein formulations and methods of making same
US9181327B2 (en) 2008-01-07 2015-11-10 The United States Of America, As Represented By The Secretary, Department Of Health And Human Services Anti-HIV domain antibodies and method of making and using same
CN101959892B (zh) 2008-02-06 2014-01-08 普罗热尼奇制药公司 (r),(r)-2,2’-二-甲基纳曲酮的制备和用途
WO2009109635A2 (en) 2008-03-05 2009-09-11 Ablynx Nv Novel antigen binding dimer-complexes, methods of making and uses thereof
US9908943B2 (en) 2008-04-03 2018-03-06 Vib Vzw Single domain antibodies capable of modulating BACE activity
GB0809069D0 (en) 2008-05-19 2008-06-25 Univ Leuven Kath Gene signatures
EP2281005B1 (de) 2008-04-03 2013-11-20 Vib Vzw Einzeldomänenantikörper, die dazu in der lage sind, die bace1-aktivität zu modulieren
CA2720763A1 (en) 2008-04-07 2009-10-15 Ablynx Nv Amino acid sequences directed against the notch pathways and uses thereof
CN102089325A (zh) 2008-04-17 2011-06-08 埃博灵克斯股份有限公司 能够结合血清白蛋白的肽,以及包含其的化合物、构建体和多肽
JP6034023B2 (ja) 2008-05-16 2016-11-30 アブリンクス エン.ヴェー. Cxcr4及び他のgpcrに指向性を有するアミノ酸配列及びそれを含む化合物
LT2285408T (lt) 2008-06-05 2019-01-25 Ablynx N.V. Aminorūgščių sekos, nukreiptos prieš viruso apvalkalo baltymus, ir tokias sekas turintys polipeptidai, skirti virusinių ligų gydymui
CA2747644C (en) 2008-12-19 2023-01-24 Ablynx N.V. Method for generation of immunoglobulin sequences
WO2010100135A1 (en) 2009-03-05 2010-09-10 Ablynx N.V. Novel antigen binding dimer-complexes, methods of making/avoiding and uses thereof
WO2010125187A2 (en) 2009-04-30 2010-11-04 Ablynx Nv Method for the production of domain antibodies
CA2762837C (en) 2009-05-20 2021-08-03 Novimmune S.A. Synthetic polypeptide libraries and methods for generating naturally diversified polypeptide variants
CA2764158A1 (en) 2009-06-01 2010-12-09 Halo-Bio Rnai Therapeutics, Inc. Polynucleotides for multivalent rna interference, compositions and methods of use thereof
HUE051430T2 (hu) 2009-07-10 2021-03-01 Ablynx Nv Eljárás variábilis domének elõállítására
PL3725330T3 (pl) 2009-09-03 2025-12-08 Ablynx N.V. Stabilne formulacje polipeptydów i ich zastosowania
US9340605B2 (en) 2009-10-22 2016-05-17 Universiteit Twente VHH for application in tissue repair, organ regeneration, organ replacement and tissue engineering
EP2506874A1 (de) 2009-12-01 2012-10-10 Ablynx N.V. Von-willebrand-faktor-spezifische bindemittel und ihre verwendung
EP3309176B1 (de) 2009-12-14 2025-10-01 Ablynx N.V. Variable einzeldomänen-antikörper gegen ox40l, konstrukte und ihre therapeutische verwendung
WO2011083141A2 (en) 2010-01-08 2011-07-14 Ablynx Nv Method for generation of immunoglobulin sequences by using lipoprotein particles
CA2788993A1 (en) 2010-02-05 2011-08-11 Ablynx N.V. Peptides capable of binding to serum albumin and compounds, constructs and polypeptides comprising the same
US9120855B2 (en) 2010-02-10 2015-09-01 Novartis Ag Biologic compounds directed against death receptor 5
HUE044292T2 (hu) 2010-02-11 2019-10-28 Ablynx Nv Eljárások és készítmények aeroszolok elõállítására
US9101674B2 (en) 2010-03-29 2015-08-11 Vib Vzw Targeting and in vivo imaging of tumor-associated macrophages
US9556273B2 (en) 2010-03-29 2017-01-31 Vib Vzw Anti-macrophage mannose receptor single variable domains for targeting and in vivo imaging of tumor-associated macrophages
AU2011254557B2 (en) 2010-05-20 2015-09-03 Ablynx Nv Biological materials related to HER3
WO2011161263A1 (en) 2010-06-25 2011-12-29 Ablynx Nv Pharmaceutical compositions for cutaneous administration
GB201014715D0 (en) 2010-09-06 2010-10-20 Vib Vzw Nanobodies stabilizing functional conformational states of GPCRS
HUE034017T2 (en) 2010-08-26 2018-01-29 Agrosavfe N V Preparations for seed treatment
DK2632946T3 (en) 2010-10-29 2018-03-12 Ablynx Nv PROCEDURE FOR MANUFACTURING VARIABLE IMMUNGLOBULIN SINGLE DOMAINS
AU2012234282B2 (en) 2011-03-28 2015-07-16 Ablynx Nv Method for producing solid formulations comprising immunoglobulin single variable domains
UA117218C2 (uk) 2011-05-05 2018-07-10 Мерк Патент Гмбх Поліпептид, спрямований проти il-17a, il-17f та/або il17-a/f
EP3590950A1 (de) 2011-05-09 2020-01-08 Ablynx NV Verfahren zur herstellung von einzelvariablen immunglobulindomänen
KR102072250B1 (ko) 2011-05-27 2020-03-02 아블린쓰 엔.브이. Rankl 결합 펩티드를 이용한 골 재흡수 억제
HUE036982T2 (hu) 2011-06-21 2018-08-28 Vib Vzw GPCR:G-fehérje komplexek ellen irányuló kötõdomének és alkalmazások
WO2012175740A1 (en) 2011-06-23 2012-12-27 Ablynx Nv Immunoglobulin single variable domains directed against ige
DK2747782T3 (en) 2011-09-23 2018-04-23 Ablynx Nv Long-term inhibition of interleukin-6-mediated signal transmission
US10112988B2 (en) 2012-01-09 2018-10-30 Icb International, Inc. Methods of assessing amyloid-beta peptides in the central nervous system by blood-brain barrier permeable peptide compositions comprising a vab domain of a camelid single domain heavy chain antibody against an anti-amyloid-beta peptide
US10112987B2 (en) 2012-01-09 2018-10-30 Icb International, Inc. Blood-brain barrier permeable peptide compositions comprising a vab domain of a camelid single domain heavy chain antibody against an amyloid-beta peptide
EP2617732A1 (de) 2012-01-19 2013-07-24 Vib Vzw Werkzeuge und Verfahren zur Expression von Membranproteinen
EP2855526B1 (de) 2012-05-24 2018-11-07 VIB vzw Einzelne immunoglobulin d'immunoglobulin variable antimakrophagen-mannose-rezeptordomänen zur anzielung und in-vivo-bildgebung von tumorassoziierten makrophagen
WO2014087010A1 (en) 2012-12-07 2014-06-12 Ablynx N.V. IMPROVED POLYPEPTIDES DIRECTED AGAINST IgE
US9593157B2 (en) 2013-01-30 2017-03-14 Vib Vzw Chimeric polypeptides comprising G protein-coupled receptors and VHH antibodies
US9553473B2 (en) 2013-02-04 2017-01-24 Ossia Inc. Systems and methods for optimally delivering pulsed wireless power
CA2900147C (en) 2013-02-05 2023-09-05 Vib Vzw Muscarinic acetylcholine receptor binding agents and uses thereof
US9617339B2 (en) 2013-03-15 2017-04-11 Vib Vzw Method of imaging a cardiovascular disease with an anti-macrophage mannose receptor immunoglobulin single variable domain
CN116076508A (zh) 2013-04-29 2023-05-09 鲁汶天主教大学 包含结合至鞘脂的抗体的农用化学组合物
NL1040254C2 (en) 2013-05-17 2014-11-24 Ablynx Nv Stable formulations of immunoglobulin single variable domains and uses thereof.
EP2883883A1 (de) 2013-12-16 2015-06-17 Cardio3 Biosciences S.A. Therapeutische Ziele und Mittel zur Behandlung von ischämiebedingten Reperfusionsschäden
EP3099707B1 (de) 2014-01-30 2021-12-29 Vib Vzw Opioidrezeptorbinder und verwendungen davon
NL2013661B1 (en) 2014-10-21 2016-10-05 Ablynx Nv KV1.3 Binding immunoglobulins.
EP3194976B1 (de) 2014-07-22 2020-04-01 Vib Vzw Verfahren zur auswahl von agenten, die proteinkomplexe stabilisieren
US20180036442A1 (en) 2014-07-29 2018-02-08 Vrije Universiteit Brussel Radio-labelled antibody fragments for use in the prognosis, diagnosis of cancer as well as for the prediction of cancer therapy response
MX356502B (es) 2014-07-29 2018-05-31 Univ Brussel Vrije Fragmentos de anticuerpos radiomarcados para usarse en la prevencion y/o tratamiento de cancer.
JP7089877B2 (ja) 2014-11-05 2022-06-23 バイオタリス・エン・フェー 重鎖抗体の可変ドメインをコードするポリヌクレオチドを含むトランスジェニック植物
EP3233910B1 (de) 2014-12-19 2019-12-11 Ablynx N.V. Cysteinverknüpfte nanokörperdimere
KR20190080992A (ko) 2015-05-21 2019-07-08 하푼 테라퓨틱스, 인크. 삼중특이성 결합 단백질 및 사용 방법
CA2991398A1 (en) 2015-07-17 2017-01-26 Vrije Universiteit Brussel Radiolabelled antibody fragments for use in treating cancer
CN105384825B (zh) 2015-08-11 2018-06-01 南京传奇生物科技有限公司 一种基于单域抗体的双特异性嵌合抗原受体及其应用
MX2018002090A (es) 2015-08-24 2018-09-12 Halo Bio Rnai Therapeutics Inc Nanoparticulas de polinucleótido para modulación de expresión génica y sus usos.
RU2018122255A (ru) 2015-11-27 2019-12-19 Аблинкс Нв Полипептиды, ингибирующие cd40l
EP3411065B1 (de) 2016-02-05 2021-03-31 Orionis Biosciences BV Clec9a liganden
US11248057B2 (en) 2016-03-07 2022-02-15 Vib Vzw CD20 binding single domain antibodies
CA3017813C (en) 2016-03-17 2021-12-07 Oslo Universitetssykehus Hf Fusion proteins targeting tumour associated macrophages for treating cancer
WO2017182603A1 (en) 2016-04-22 2017-10-26 Université Libre de Bruxelles A new biomarker expressed in pancreatic beta cells useful in imaging or targeting beta cells
WO2017182605A1 (en) 2016-04-22 2017-10-26 Université Libre de Bruxelles A new biomarker expressed in pancreatic beta cells useful in imaging or targeting beta cells
US20190127447A1 (en) 2016-05-02 2019-05-02 Ablynx N.V. Treatment of rsv infection
EP3455245A2 (de) 2016-05-13 2019-03-20 Orionis Biosciences NV Therapeutisches targeting von nichtzellulären strukturen
US11623958B2 (en) 2016-05-20 2023-04-11 Harpoon Therapeutics, Inc. Single chain variable fragment CD3 binding proteins
EP3493844A4 (de) 2016-05-20 2021-03-24 Harpoon Therapeutics Inc. Einzeldomänen-serumalbuminbindendes protein
PL3461261T3 (pl) 2016-05-20 2025-11-12 Harpoon Therapeutics, Inc. Białka wiążące jednołańcuchowy fragment zmienny CD3
WO2018007442A1 (en) 2016-07-06 2018-01-11 Ablynx N.V. Treatment of il-6r related diseases
WO2018014260A1 (en) 2016-07-20 2018-01-25 Nanjing Legend Biotech Co., Ltd. Multispecific antigen binding proteins and methods of use thereof
WO2018029182A1 (en) 2016-08-08 2018-02-15 Ablynx N.V. Il-6r single variable domain antibodies for treatment of il-6r related diseases
EP3512880A1 (de) 2016-09-15 2019-07-24 Ablynx NV Gegen makrophagenmigrationshemmenden faktor gerichte variable immunglobulin-einzeldomänen
WO2018068201A1 (en) 2016-10-11 2018-04-19 Nanjing Legend Biotech Co., Ltd. Single-domain antibodies and variants thereof against ctla-4
US10968281B2 (en) * 2016-10-14 2021-04-06 Institut Curie Anti-LSP1 antibody
CN110177809B (zh) 2016-11-16 2023-11-03 埃博灵克斯股份有限公司 能够结合CD123和TCRα/β的T细胞募集多肽
MX2019006045A (es) 2016-11-23 2019-11-11 Harpoon Therapeutics Inc Proteinas triespecificas dirigidas a psma y metodos de uso.
KR20210087108A (ko) 2016-11-23 2021-07-09 하푼 테라퓨틱스, 인크. 전립선 특이 막 항원 결합 단백질
WO2018099968A1 (en) 2016-11-29 2018-06-07 Ablynx N.V. Treatment of infection by respiratory syncytial virus (rsv)
EP3577133A1 (de) 2017-02-06 2019-12-11 Orionis Biosciences NV Gezielte chimäre proteine und verwendungen davon
US11535668B2 (en) 2017-02-28 2022-12-27 Harpoon Therapeutics, Inc. Inducible monovalent antigen binding protein
JP7186401B2 (ja) 2017-02-28 2022-12-09 フエー・イー・ベー・フエー・ゼツト・ウエー タンパク質の経口送達のための手段及び方法
WO2018192974A1 (en) 2017-04-18 2018-10-25 Université Libre de Bruxelles Biomarkers and targets for proliferative diseases
JP7678244B2 (ja) 2017-05-11 2025-05-16 ブイアイビー ブイゼットダブリュ 可変免疫グロブリンドメインのグリコシル化
SG10202107880XA (en) 2017-05-12 2021-09-29 Harpoon Therapeutics Inc Mesothelin binding proteins
CA3063362A1 (en) 2017-05-12 2018-11-15 Harpoon Therapeutics, Inc. Msln targeting trispecific proteins and methods of use
WO2018222587A1 (en) 2017-05-30 2018-12-06 The Regents Of The University Of California NANOBODIES AGAINST CYSTIC FIBROSIS TRANSMEMBRANE CONDUCTANCE REGULATOR (CFTR) INHIBITORY FACTOR (Cif)
AU2018277310B2 (en) 2017-06-02 2024-07-11 Ablynx Nv Aggrecan binding immunoglobulins
HRP20241562T1 (hr) 2017-06-02 2025-01-31 Merck Patent Gmbh Adamts vezujući imunoglobulini
JP7249961B2 (ja) 2017-06-02 2023-03-31 メルク パテント ゲゼルシャフト ミット ベシュレンクテル ハフツング Adamts5、mmp13およびアグリカンに結合するポリペプチド
TW202417517A (zh) 2017-06-02 2024-05-01 德商麥克專利有限公司 與mmp13結合之免疫球蛋白
WO2019000223A1 (en) 2017-06-27 2019-01-03 Nanjing Legend Biotech Co., Ltd. ENABLERS OF IMMUNE EFFECTOR CELLS OF CHIMERIC ANTIBODIES AND METHODS OF USE THEREOF
KR102625929B1 (ko) 2017-07-19 2024-01-16 브이아이비 브이지더블유 혈청 알부민 결합제
PL3694529T3 (pl) 2017-10-13 2024-12-16 Harpoon Therapeutics, Inc. Trójswoiste białka i sposoby zastosowania
IL315737A (en) 2017-10-13 2024-11-01 Harpoon Therapeutics Inc B-cell maturation antigen-binding proteins
WO2019086548A1 (en) 2017-10-31 2019-05-09 Vib Vzw Novel antigen-binding chimeric proteins and methods and uses thereof
JP7369127B2 (ja) 2017-12-28 2023-10-25 ナンジン レジェンド バイオテック カンパニー,リミテッド Tigitに対する単一ドメイン抗体及びその変異体
KR102839330B1 (ko) 2018-01-15 2025-07-30 난징 레전드 바이오테크 씨오., 엘티디. Pd-1에 대한 단일-도메인 항체 및 이의 변이체
WO2019148089A1 (en) 2018-01-26 2019-08-01 Orionis Biosciences Inc. Xcr1 binding agents and uses thereof
WO2019152979A1 (en) 2018-02-05 2019-08-08 Orionis Biosciences, Inc. Fibroblast binding agents and use thereof
WO2019155041A1 (en) 2018-02-12 2019-08-15 Vib Vzw Gβγ COMPLEX ANTIBODIES AND USES THEREOF
CA3092421A1 (en) 2018-03-01 2019-09-06 Vrije Universiteit Brussel Human pd-l1-binding immunoglobulins
WO2019175131A1 (en) * 2018-03-14 2019-09-19 F. Hoffmann-La Roche Ag Method for affinity maturation of antibodies
EP3768701B1 (de) 2018-03-23 2023-08-02 Université Libre de Bruxelles Agonistenmoleküle des wnt-signalwegs
JP2021519093A (ja) 2018-03-27 2021-08-10 ユーエムシー ユトレヒト ホールディング ビー.ブイ. 微小血管血栓症の処置のための標的化血栓溶解
US11958903B2 (en) 2018-03-30 2024-04-16 Nanjing Legend Biotech Co., Ltd. Single-domain antibodies against LAG-3 and uses thereof
MX2020012217A (es) 2018-05-14 2021-03-02 Harpoon Therapeutics Inc Porción de unión para activación condicional de moléculas de inmunoglobulina.
US12195544B2 (en) 2018-09-21 2025-01-14 Harpoon Therapeutics, Inc. EGFR binding proteins and methods of use
CN113286817B (zh) 2018-09-25 2025-01-28 哈普恩治疗公司 Dll3结合蛋白及使用方法
US12410225B2 (en) 2018-11-08 2025-09-09 Orionis Biosciences, Inc Modulation of dendritic cell lineages
JP2022521551A (ja) 2019-02-26 2022-04-08 ツイスト バイオサイエンス コーポレーション Glp1受容体の変異体核酸ライブラリ
EP3963328A1 (de) 2019-04-29 2022-03-09 Confo Therapeutics N.V. Chimäre proteine und verfahren zum screening von verbindungen und liganden, die an gpcrs binden
WO2020221888A1 (en) 2019-04-30 2020-11-05 Vib Vzw Cystic fibrosis transmembrane conductance regulator stabilizing agents
MX2021014007A (es) 2019-05-14 2022-01-24 Harpoon Therapeutics Inc Proteinas de union a epcam y metodos de uso.
EP3976650A1 (de) 2019-05-28 2022-04-06 Vib Vzw Krebsbehandlung durch abzielen auf plexine im immunbereich
US12472254B2 (en) 2019-05-28 2025-11-18 Vib Vzw CD8+ T-cells lacking plexins and their application in cancer treatment
EP4034564A4 (de) 2019-09-23 2023-12-13 Twist Bioscience Corporation Variante nukleinsäurebibliotheken für einzeldomänenantikörper
US20220380456A1 (en) 2019-10-21 2022-12-01 Vib Vzw Nanodisc-specific antigen-binding chimeric proteins
WO2021095031A2 (en) 2019-11-11 2021-05-20 Ibi-Ag Innovative Bio Insecticides Ltd. Insect control nanobodies and uses thereof
CA3158991A1 (en) 2019-11-27 2021-06-03 Vib Vzw Positive allosteric modulators of the calcium-sensing receptor
GB201918279D0 (en) 2019-12-12 2020-01-29 Vib Vzw Glycosylated single chain immunoglobulin domains
EP4077372A1 (de) 2019-12-20 2022-10-26 Vib Vzw Nanokörperaustauschchromatographie
WO2021140205A1 (en) 2020-01-10 2021-07-15 Confo Therapeutics N.V. Methods for generating antibodies and antibody fragments and libraries comprising same
WO2021156490A2 (en) 2020-02-06 2021-08-12 Vib Vzw Corona virus binders
KR20220144841A (ko) 2020-02-21 2022-10-27 하푼 테라퓨틱스, 인크. Flt3 결합 단백질 및 사용 방법
US20230087785A1 (en) 2020-02-25 2023-03-23 Vib Vzw Leucine-Rich Repeat Kinase 2 Allosteric Modulators
WO2021198396A1 (en) 2020-03-31 2021-10-07 Biotalys NV Anti-fungal polypeptides
JP2023523600A (ja) 2020-04-22 2023-06-06 マブウェル (シャンハイ) バイオサイエンス カンパニー リミテッド ヒトプログラム細胞死リガンド1(pd-l1)を標的とする単一可変ドメイン抗体およびその誘導体
WO2021229104A1 (en) 2020-05-15 2021-11-18 Université de Liège Anti-cd38 single-domain antibodies in disease monitoring and treatment
US11981932B2 (en) 2020-06-17 2024-05-14 Janssen Biotech, Inc. Materials and methods for the manufacture of pluripotent stem cells
WO2022003156A1 (en) 2020-07-02 2022-01-06 Oncurious Nv Ccr8 non-blocking binders
WO2022023584A1 (en) 2020-07-31 2022-02-03 Biotalys NV Methods of increasing recombinant protein yields
AU2021331088A1 (en) 2020-08-26 2023-05-04 Twist Bioscience Corporation Methods and compositions relating to glp1r variants
WO2022063947A1 (en) 2020-09-24 2022-03-31 Vib Vzw Combination of p2y6 inhibitors and immune checkpoint inhibitors
WO2022063957A1 (en) 2020-09-24 2022-03-31 Vib Vzw Biomarker for anti-tumor therapy
BR112023005273A2 (pt) 2020-09-25 2023-04-25 Ablynx Nv Polipeptídeos compreendendo domínios variáveis únicos de imunoglobulina direcionados a il-13 e ox40l
WO2022117569A1 (en) 2020-12-02 2022-06-09 Oncurious Nv A ccr8 antagonist antibody in combination with a lymphotoxin beta receptor agonist antibody in therapy against cancer
WO2022117572A2 (en) 2020-12-02 2022-06-09 Oncurious Nv An ltbr agonist in combination therapy against cancer
KR20230123497A (ko) 2020-12-18 2023-08-23 아블린쓰 엔.브이. IL-6 및 TNF-α를 표적화하는 면역글로불린 단일 가변도메인을 포함하는 폴리펩티드
GB202020502D0 (en) 2020-12-23 2021-02-03 Vib Vzw Antibody composistion for treatment of corona virus infection
EP4267618A1 (de) 2020-12-24 2023-11-01 Vib Vzw Nichtblockierende, menschliche ccr8-bindemittel
US20240052045A1 (en) 2020-12-24 2024-02-15 Vib Vzw Murine cross-reactive human ccr8 binders
EP4267617A1 (de) 2020-12-24 2023-11-01 Vib Vzw Menschliche ccr8-bindemittel
WO2022156908A1 (en) 2021-01-25 2022-07-28 Vrije Universiteit Brussel Method for preparing a lyophilized composition
WO2022156907A1 (en) 2021-01-25 2022-07-28 Vrije Universiteit Brussel Method and kit for labeling a biomolecule with one or more detectable labels, including a radiolabel
WO2022157373A1 (en) 2021-01-25 2022-07-28 Vrije Universiteit Brussel Compositions and kits for in vivo imaging of cardiac sarcoidosis
CN117794566A (zh) 2021-02-05 2024-03-29 Vib研究所 沙贝病毒结合剂
AU2022216460A1 (en) 2021-02-05 2023-09-21 Universiteit Gent Sarbecovirus binders
US20240130999A1 (en) 2021-02-17 2024-04-25 Vib Vzw Inhibition of SLC4A4 in the Treatment of Cancer
KR102836732B1 (ko) 2021-02-19 2025-07-21 (주)샤페론 Pd-l1에 대한 단일 도메인 항체 및 이의 용도
EP4294516A1 (de) 2021-02-19 2023-12-27 Vib Vzw Kationunabhängige mannose-6-phosphatrezeptorbindemittel
KR102862614B1 (ko) 2021-02-19 2025-09-22 (주)샤페론 Cd47에 대한 단일 도메인 항체 및 이의 용도
JP7773239B2 (ja) 2021-02-19 2025-11-19 シャペロン インク. Pd-l1及びcd47に対する二重特異性単一ドメイン抗体及びその用途
CN117321076A (zh) 2021-02-19 2023-12-29 美国卫生及公众服务部代表 中和SARS-CoV-2的单结构域抗体
WO2022199804A1 (en) 2021-03-24 2022-09-29 Vib Vzw Nek6 inhibition to treat als and ftd
US20240261446A1 (en) 2021-05-17 2024-08-08 Université de Liège Anti-cd38 single domain antibodies in disease monitoring and treatment
EP4359421A1 (de) 2021-06-23 2024-05-01 Vib Vzw Mittel und verfahren zur auswahl spezifischer bindemittel
WO2022269473A1 (en) 2021-06-23 2022-12-29 Janssen Biotech, Inc. Materials and methods for hinge regions in functional exogenous receptors
CN117580865A (zh) 2021-06-29 2024-02-20 山东先声生物制药有限公司 Cd16抗体及其应用
WO2023016828A2 (en) 2021-07-30 2023-02-16 Vib Vzw Cation-independent mannose-6-phosphate receptor binders for targeted protein degradation
JP7727086B2 (ja) 2021-07-30 2025-08-20 山▲東▼先声生物制▲薬▼有限公司 抗pvrig/抗tigit二重特異性抗体及び応用
US20250009908A1 (en) 2021-10-05 2025-01-09 Vrije Universiteit Brussel Fluorescently Labeled Immunoglobulin Single Variable Domains
WO2023057601A1 (en) 2021-10-06 2023-04-13 Biotalys NV Anti-fungal polypeptides
WO2023098846A1 (zh) 2021-12-03 2023-06-08 江苏先声药业有限公司 抗bcma纳米抗体及其应用
TW202342508A (zh) 2021-12-17 2023-11-01 比利時商艾伯霖克斯公司 包含靶向TCRαβ、CD33和CD123的免疫球蛋白單可變結構域的多肽
WO2023125888A1 (zh) 2021-12-31 2023-07-06 山东先声生物制药有限公司 一种gprc5d抗体及其应用
EP4463479A1 (de) 2022-01-12 2024-11-20 Vib Vzw Humane ntcp-bindemittel für therapeutische verwendung und leberspezifische gezielte abgabe
US20250129387A1 (en) 2022-02-02 2025-04-24 Biotalys NV Methods for genome editing
EP4476250A1 (de) 2022-02-07 2024-12-18 Vib Vzw Gentechnisch veränderte stabilisierung aglycosylierter fc-regionen
KR20240154657A (ko) 2022-03-07 2024-10-25 다케다 야쿠힌 고교 가부시키가이샤 임상 인간 igg 제품의 친화도 크로마토그래피 생산
WO2023198848A1 (en) 2022-04-13 2023-10-19 Vib Vzw An ltbr agonist in combination therapy against cancer
US20250289904A1 (en) 2022-05-02 2025-09-18 Umc Utrecht Holding B.V. Single domain antibodies for the detection of plasmin-cleaved vwf
US20250313611A1 (en) 2022-05-18 2025-10-09 Vib Vzw Sarbecovirus spike s2 subunit binders
IL317463A (en) 2022-06-06 2025-02-01 Shandong Simcere Biopharmaceutical Co Ltd Multispecific antibodies targeting BCMA, GPRC5D and T cells and their application
CN115012043A (zh) * 2022-07-01 2022-09-06 南京瑞源生物技术有限公司 一种构建纳米抗体文库的方法
EP4551600A1 (de) 2022-07-04 2025-05-14 Vib Vzw Blut-cerebrospinal-fluidbarriere-überkreuzende antikörper
US20250188492A1 (en) 2022-07-22 2025-06-12 Janssen Biotech, Inc. Enhanced Transfer of Genetic Instructions to Effector Immune Cells
WO2024068744A1 (en) 2022-09-27 2024-04-04 Vib Vzw Antivirals against human parainfluenza virus
EP4349374A1 (de) 2022-10-05 2024-04-10 Vrije Universiteit Brussel Variable einzeldomänen des anti-urokinase-plasminogenaktivatorrezeptors immunglobulin
WO2024083843A1 (en) 2022-10-18 2024-04-25 Confo Therapeutics N.V. Amino acid sequences directed against the melanocortin 4 receptor and polypeptides comprising the same for the treatment of mc4r-related diseases and disorders
JP2025537809A (ja) 2022-11-15 2025-11-20 アイメック・ヴェーゼットウェー 液滴操作のための方法およびシステム
AR131388A1 (es) 2022-12-15 2025-03-12 Univ Aarhus Activación sintética de receptores transmembrana multiméricos
EP4638735A1 (de) 2022-12-22 2025-10-29 Biotalys NV Verfahren zur genomeditierung
WO2024145551A1 (en) 2022-12-29 2024-07-04 Biotalys NV Agrochemical compositions
EP4642916A2 (de) 2022-12-30 2025-11-05 Biotalys NV Sekretionssignale
EP4642233A1 (de) 2022-12-30 2025-11-05 Biotalys NV Agglomerat
WO2024141638A1 (en) 2022-12-30 2024-07-04 Biotalys NV Self-emulsifiable concentrate
EP4655324A1 (de) 2023-01-27 2025-12-03 Vib Vzw Cd163-bindende konjugate
WO2024156881A1 (en) 2023-01-27 2024-08-02 Vib Vzw CD8b-BINDING POLYPEPTIDES
EP4662246A1 (de) 2023-02-09 2025-12-17 Seni-Preps B.V. Variable immunglobulin-einzeldomänen zur hemmung von urease und verwendung davon
WO2024175787A1 (en) 2023-02-24 2024-08-29 Vrije Universiteit Brussel Anti-inflammatory pannexin 1 channel inhibitors
US20240344078A1 (en) 2023-03-14 2024-10-17 Aarhus Universitet Genetically altered nfr5 receptor kinases
AU2024243709A1 (en) 2023-04-03 2025-11-06 Katholieke Universiteit Leuven Blood-brain barrier crossing antibodies
WO2024231348A1 (en) 2023-05-11 2024-11-14 Vib Vzw Slc4a4/nbce1 inhibitors
WO2024240162A1 (en) 2023-05-23 2024-11-28 Shanghai Allygen Biologics Co., Ltd. Pd-l1 and trop-2 targeting conjugates comprising effector molecules and uses thereof
WO2024261344A1 (en) 2023-06-23 2024-12-26 Vib Vzw Novel binders targeting the multi-drug resistant pathogen acinetobacter baumannii
EP4483951A1 (de) 2023-06-30 2025-01-01 Université de Liège Einzeldomänenantikörper zur hemmung der aktivität neutrophiler elastase
WO2025034806A1 (en) 2023-08-08 2025-02-13 Wisconsin Alumni Research Foundation Single-domain antibodies and variants thereof against fibroblast activation protein
NL2036011B1 (en) 2023-10-12 2025-04-30 Synapse Res Institute Molecules for reversing anti-coagulant activity of direct oral anticoagulants
WO2025088085A1 (en) 2023-10-26 2025-05-01 Abscint Nv Image guided biopsy of her2 positive lesions
WO2025093683A1 (en) 2023-11-03 2025-05-08 Neuvasq Biotechnologies Sa Wnt7 signaling agonists
WO2025109176A1 (en) 2023-11-22 2025-05-30 Exevir Bio Bv Optimized sarbecovirus spike s2 subunit binders and compositions comprising the same
WO2025125577A1 (en) 2023-12-14 2025-06-19 Vib Vzw Antibodies against influenza b virus
EP4574981A1 (de) 2023-12-22 2025-06-25 Biotalys NV Anti-fungale vhh-antikörper
WO2025154056A1 (en) 2024-01-21 2025-07-24 Ibi-Ag Innovative Bio Insecticides Ltd. Anti-insect cda nanobodies and uses thereof
WO2025154058A1 (en) 2024-01-21 2025-07-24 Ibi-Ag Innovative Bio Insecticides Ltd. Anti-insect hsp70 nanobodies and uses thereof
WO2025178959A1 (en) 2024-02-20 2025-08-28 University Of Georgia Research Foundation, Inc. Single-domain antibodies and variants thereof against tab1
WO2025181155A1 (en) 2024-02-26 2025-09-04 Vib Vzw Human beta-glucocerebrosidase binders and uses thereof
WO2025196308A1 (en) 2024-03-22 2025-09-25 Vib Vzw Means and methods for displaying fc-containing proteins on cells and selection thereof
WO2025219231A1 (en) 2024-04-15 2025-10-23 Vib Vzw Computer-implemented means and methods for the de novo design of antibodies targeting a specific epitope
WO2025238157A1 (en) 2024-05-15 2025-11-20 Katholieke Universiteit Leuven Multispecific binding agent suitable for use in cancer immune therapy

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0584421A1 (de) * 1992-08-21 1994-03-02 Cécile Casterman Immunoglobuline ohne Leichtkette
ES2162863T3 (es) * 1993-04-29 2002-01-16 Unilever Nv Produccion de anticuerpos o fragmentos (funcionalizados) de los mismos derivados de inmunoglobulinas de cadena pesada de camelidae.

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