Classifications

• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
  • A61K31/00—Medicinal preparations containing organic active ingredients
  • A61K31/33—Heterocyclic compounds
  • A61K31/395—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins
  • A61K31/435—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having six-membered rings with one nitrogen as the only ring hetero atom
  • A61K31/44—Non condensed pyridines; Hydrogenated derivatives thereof
  • A61K31/4427—Non condensed pyridines; Hydrogenated derivatives thereof containing further heterocyclic ring systems
  • A61K31/4439—Non condensed pyridines; Hydrogenated derivatives thereof containing further heterocyclic ring systems containing a five-membered ring with nitrogen as a ring hetero atom, e.g. omeprazole
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
  • A61K31/00—Medicinal preparations containing organic active ingredients
  • A61K31/33—Heterocyclic compounds
  • A61K31/395—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins
  • A61K31/435—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having six-membered rings with one nitrogen as the only ring hetero atom
  • A61K31/44—Non condensed pyridines; Hydrogenated derivatives thereof
  • A61K31/4427—Non condensed pyridines; Hydrogenated derivatives thereof containing further heterocyclic ring systems
  • A61K31/444—Non condensed pyridines; Hydrogenated derivatives thereof containing further heterocyclic ring systems containing a six-membered ring with nitrogen as a ring heteroatom, e.g. amrinone
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
  • A61K31/00—Medicinal preparations containing organic active ingredients
  • A61K31/70—Carbohydrates; Sugars; Derivatives thereof
  • A61K31/7042—Compounds having saccharide radicals and heterocyclic rings
  • A61K31/7052—Compounds having saccharide radicals and heterocyclic rings having nitrogen as a ring hetero atom, e.g. nucleosides, nucleotides
  • A61K31/706—Compounds having saccharide radicals and heterocyclic rings having nitrogen as a ring hetero atom, e.g. nucleosides, nucleotides containing six-membered rings with nitrogen as a ring hetero atom
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
  • A61K45/00—Medicinal preparations containing active ingredients not provided for in groups A61K31/00 - A61K41/00
  • A61K45/06—Mixtures of active ingredients without chemical characterisation, e.g. antiphlogistics and cardiaca
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
  • A61P35/00—Antineoplastic agents
  • A61P35/02—Antineoplastic agents specific for leukemia
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
  • A61K2300/00—Mixtures or combinations of active ingredients, wherein at least one active ingredient is fully defined in groups A61K31/00 - A61K41/00

Definitions

• the therapies involve treatment with an IDH1 inhibitor and a DNA demethylating agent.
• Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate (i.e., a-ketoglutarate). These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+).
• NAD(+) the electron acceptor
• NADP(+) the NADP(+)-dependent isocitrate dehydrogenases
• Each NADP(+)-dependent isozyme is a homodimer.
• IDH1 isocitrate dehydrogenase 1 (NADP+), cytosolic
• IDP isocitrate dehydrogenase 1
• IDCD isocitrate dehydrogenase 1
• PICD PICD
• the protein encoded by this gene is the NADP(+) -dependent isocitrate dehydrogenase found in the cytoplasm and peroxisomes. It contains the PTS-1 peroxisomal targeting signal sequence.
• the presence of this enzyme in peroxisomes suggests roles in the regeneration of NADPH for intraperoxisomal reductions, such as the conversion of 2, 4-dienoyl- CoAs to 3-enoyl-CoAs, as well as in peroxisomal reactions that consume 2-oxoglutarate, namely the alpha-hydroxylation of phytanic acid.
• the cytoplasmic enzyme serves a significant role in cytoplasmic NADPH production.
• the human IDH1 gene encodes a protein of 414 amino acids.
• the nucleotide and amino acid sequences for human IDH1 can be found as GenBank entries NM_005896.2 and NP_005887.2 respectively.
• the nucleotide and amino acid sequences for IDH1 are also described in, e.g., Nekrutenko et ah, Mol. Biol. Evol. 15: 1674-1684(1998); Geisbrecht et ah, J. Biol. Chem. 274:30527-30533(1999); Wiemann et ah, Genome Res. 11:422-435(2001); The MGC Project Team, Genome Res.
• Non-mutant e.g., wild type, IDHl catalyzes the oxidative decarboxylation of isocitrate to a-ketoglutarate thereby reducing NAD + (NADP + ) to NADH (NADPH), e.g., in the forward reaction:
• the hematologic malignancy is an advanced hematologic malignancy.
• provided herein is a method of treating hematologic
• lymphoma e.g., T-cell lymphoma
• AML acute myelogenous leukemia
• MDS myelodysplastic syndrome
• MDS myeloproliferative neoplasms
• CMML chronic myelomonocytic leukemia
• B-ALL B-acute lymphoblastic leukemias
• lymphoma e.g., T-cell lymphoma
• the DNA demethylating agent is a cytidine analog.
• cytidine analogs useful in the methods provided herein include, but are not limited to, 5-azacitidine (azacitidine), 5-azadeoxycytidine (decitabine), cytarabine, pseudoisocytidine, gemcitabine, zebularine, FCdR, Emtriva, 5,6-dihydro-5-azacitidine and procaine.
• the cytidine analog is decitabine or azacitidine.
• the cytidine analog is azacitidine.
• provided herein is a method of treating hematologic
• hematologic malignancies such as acute myelogenous leukemia (AML), myelodysplastic syndrome (MDS), myeloproliferative neoplasms (MPN), chronic myelomonocytic leukemia (CMML), B-acute lymphoblastic leukemias (B-ALL), or lymphoma (e.g., T-cell lymphoma), each characterized by the presence of a mutant allele of IDHl, comprising administering to a subject a therapeutically effective amount of COMPOUND 2 and azacitidine.
• the hematologic malignancy is an advanced hematologic malignancy.
• provided herein is a method of treating hematologic
• hematologic malignancies such as acute myelogenous leukemia (AML), myelodysplastic syndrome (MDS), myeloproliferative neoplasms (MPN), chronic myelomonocytic leukemia (CMML), B-acute lymphoblastic leukemias (B-ALL), or lymphoma (e.g., T-cell lymphoma), each characterized by the presence of a mutant allele of IDHl, comprising administering to a subject a pharmaceutical composition comprising a therapeutically effective amount of COMPOUND 2 and a cytidine analog.
• the hematologic malignancy is an advanced hematologic malignancy.
• provided herein is a method of treating hematologic
• hematologic malignancy is an advanced hematologic malignancy.
• a method of treating solid tumors such as glioma, melanoma, chondrosarcoma, cholangiocarcinoma (including intrahepatic
• IHCC cholangiocarcinoma
• NSCLC non-small cell lung cancer
• a method of treating solid tumors such as glioma, melanoma, chondrosarcoma, cholangiocarcinoma (including intrahepatic
• IHCC cholangiocarcinoma
• NSCLC non-small cell lung cancer
• a method of treating solid tumors such as glioma, melanoma, chondrosarcoma, cholangiocarcinoma (including intrahepatic
• IHCC cholangiocarcinoma
• NSCLC non-small cell lung cancer
• composition comprising a therapeutically effective amount of COMPOUND 2 and azacitidine.
• a pharmaceutical composition comprising a therapeutically effective amount of COMPOUND 2 and a cytidine analog.
• a pharmaceutical composition comprising a therapeutically effective amount of COMPOUND 2 and azacitidine.
• FIG. 1A depicts COMPOUND 2 and azacitidine combination schedule for sequential treatment: 3 days (QDx3) of pre-treatment with azacitidine (AZA), followed by treatment with the COMPOUND 2 for 7 days, followed by erythropoietin (EPO) + COMPOUND 2 for another 7 days. The cells were harvested on Day 18 and subjected to various endpoint assays for monitoring differentiation and death.
• FIG. IB depicts COMPOUND 2 and azacitidine combination schedule for concurrent treatment: treatment for 7 days with the combination of azacitidine and COMPOUND 2, followed by 7 days treatment with azacitidine, COMPOUND 2 and EPO.
• FIG. 2A depicts the effect of azacitidine, COMPOUND 2 and a concurrent combination of azacitidine and COMPOUND 2 on cell pellet color, with red color indicating hemoglobinization. Azacitidine had little or no effect on cell pellet color; however, with the azacitidine + COMPOUND 2 combination, coloration/hemoglobinization was noticeably greater than with COMPOUND 2 alone.
• FIG. 2B depicts similar differentiation effect with a sequential schedule of azacitidine and COMPOUND 2.
• FIG. 2C depicts effect of concurrent and sequential schedules on differentiation marker CD235a (Glycophorin A). Enhanced CD235a expression was observed in cells treated with the concurrent combination of azacitidine + COMPOUND 2, as compared to single agents.
• FIG. 3A depicts the effect on RNA expression of differentiation marker HBG with single agents azacitidine and COMPOUND 2, and concurrent combination of azacitidine + COMPOUND 2.
• FIG. 3B depicts the effect on RNA expression of differentiation marker KLF1 with single agents azacitidine and COMPOUND 2, and concurrent combination of azacitidine + COMPOUND 2.
• FIG. 3C depicts the effect on RNA expression of differentiation marker HBG with single agents AZA and COMPOUND 2, and sequential combination of azacitidine + COMPOUND 2.
• FIG. 3D depicts the effect on RNA expression of differentiation marker KLF1 with single agents azacitidine and COMPOUND 2, and concurrent combination of azacitidine + COMPOUND 2.
• FIG. 4 depicts the effect of the combination of azacitidine and COMPOUND 2 on real time growth and apoptosis of TF-1 R132H cells.
• FIG. 5 is an X-ray powder diffractogram (XPRD) of COMPOUND 2 form 1.
• FIG. 6 is a differential scanning calorimetry (DSC) profile of DSC
• FIG. 7 is a thermal gravimetric analysis (TGA) profile of TGA
• FIG. 8 is an X-ray powder diffractogram (XPRD) of COMPOUND 2 form 2.
• FIG. 9 is a differential scanning calorimetry (DSC) profile of
• FIG. 10 is a thermal gravimetric analysis (TGA) profile of
• a "mutant IDHl inhibitor” or “inhibitor of IDHl mutant(s)” means a molecule e.g., a polypeptide, peptide, or small molecule (e.g., a molecule of less than 1,000 daltons), or aptomer, that binds to an IDHl mutant subunit and inhibits neoactivity, e.g., by inhibiting formation of a dimer, e.g., a homodimer of mutant IDHl subunits or a heterodimer of a mutant and a wildype subunit.
• the neoactivity inhibition is at least about 60%, 70%, 80%, 90%, 95% or 99% as compared to the activity in the absence of the mutant IDHl inhibitor.
• the mutant IDHl inhibitor is COMPOUND 2.
• the term “elevated levels of 2HG” means 10%, 20% 30%, 50%, 75%, 100%, 200%, 500% or more 2HG is present in a subject that carries a mutant IDHl allele than is present in a subject that does not carry a mutant IDHl allele.
• the term “elevated levels of 2HG” may refer to the amount of 2HG within a cell, within a tumor, within an organ comprising a tumor, or within a bodily fluid.
• the term "bodily fluid” includes one or more of amniotic fluid surrounding a fetus, aqueous humour, blood (e.g., blood plasma), serum, Cerebrospinal fluid, cerumen, chyme, Cowper's fluid, female ejaculate, interstitial fluid, lymph, breast milk, mucus (e.g., nasal drainage or phlegm), pleural fluid, pus, saliva, sebum, semen, serum, sweat, tears, urine, vaginal secretion, or vomit.
• blood e.g., blood plasma
• serum Cerebrospinal fluid
• cerumen cerumen
• chyme chyme
• Cowper's fluid female ejaculate, interstitial fluid
• lymph lymph
• mucus e.g., nasal drainage or phlegm
• pleural fluid pus, saliva, sebum, semen, serum, sweat, tears, urine, vaginal secretion, or vomit.
• inhibitor or “prevent” include both complete and partial inhibition and prevention.
• An inhibitor may completely or partially inhibit the intended target.
• the term "subject” is intended to include human and non-human animals.
• exemplary human subjects include a human patient (referred to as a patient) having a disorder, e.g., a disorder described herein or a normal subject.
• non-human animals of one aspect of the invention includes all vertebrates, e.g., non-mammals (such as chickens, amphibians, reptiles) and mammals, such as non-human primates, domesticated and/or agriculturally useful animals, e.g., sheep, dog, cat, cow, pig, etc.
• treat means decrease, suppress, attenuate, diminish, arrest, or stabilize the development or progression of a disease/disorder (e.g., an hematologic malignancy, including an advanced hematologic malignancy, such as acute myelogenous leukemia (AML),
• a disease/disorder e.g., an hematologic malignancy, including an advanced hematologic malignancy, such as acute myelogenous leukemia (AML)
• MDS myelodysplastic syndrome
• MPN myeloproliferative neoplasms
• CMML myelomonocytic leukemia
• B-ALL B-acute lymphoblastic leukemias
• lymphoma e.g., T-cell lymphoma
• solid tumor including glioma, melanoma, chondrosarcoma, cholangiocarcinoma (including intrahepatic cholangiocarcinoma (IHCC), prostate cancer, colon cancer, or non-small cell lung cancer (NSCLC), each characterized by the presence of a mutant allele of IDH1), lessen the severity of the disease/disorder or improve the symptoms associated with the disease/disorder.
• IHCC intrahepatic cholangiocarcinoma
• NSCLC non-small cell lung cancer
• An amount of a compound, including a pharmaceutically acceptable salt, solvate, tautomer, stereoisomer, isotopologue, prodrug or a polymorph thereof, effective to treat a disorder or a "therapeutically effective amount” or “therapeutically effective dose” refers to an amount of the compound, including a pharmaceutically acceptable salt, solvate, tautomer, stereoisomer, isotopologue, prodrug, or a polymorph thereof, which is effective, upon single or multiple dose administration to a subject, in treating a cell, or in curing, alleviating, relieving or improving a subject with a disorder beyond that expected in the absence of such treatment.
• co-administering means that the additional cancer therapeutic agent may be administered together with a compound provided herein as part of a single dosage form (such as a composition comprising a compound and a second therapeutic agent as described above) or as separate, multiple dosage forms.
• the additional cancer therapeutic agent may be administered together with a compound provided herein as part of a single dosage form (such as a composition comprising a compound and a second therapeutic agent as described above) or as separate, multiple dosage forms.
• the additional cancer therapeutic agent may be administered together with a compound provided herein as part of a single dosage form (such as a composition comprising a compound and a second therapeutic agent as described above) or as separate, multiple dosage forms.
• the additional cancer therapeutic agent may be
• both the compounds provided herein and the second therapeutic agent(s) are administered by conventional methods.
• composition comprising both a compound provided herein and a second therapeutic agent
• administration of a composition does not preclude the separate administration of that same therapeutic agent, any other second therapeutic agent or any compound provided herein to said subject at another time during a course of treatment.
• co-administering as used herein with respect to an additional cancer treatment means that the additional cancer treatment may occur prior to, consecutively with, concurrently with or following the administration of a compound provided herein.
• DNA demethylating agent refers to an agent that inhibits the transfer of a methyl group to DNA.
• the DNA demethylating agent is a cytidine analog.
• a cytidine analog referred to herein is intended to encompass the free base of the cytidine analog, or a salt, solvate, hydrate, cocrystal, complex, prodrug, precursor, metabolite, and/or derivative thereof.
• a cytidine analog referred to herein encompasses the free base of the cytidine analog, or a salt, solvate, hydrate, cocrystal or complex thereof.
• a cytidine analog referred to herein encompasses the free base of the cytidine analog, or a pharmaceutically acceptable salt, solvate, or hydrate thereof.
• substantially free of other stereoisomers means a preparation enriched in a compound having a selected stereochemistry at one or more selected stereocenters by at least about 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95%, 96%, 97%, 98%, or 99%.
• enriched means that at least the designated percentage of a preparation is the compound having a selected stereochemistry at one or more selected stereocenters.
• crystalline refers to a solid having a highly regular chemical structure.
• a crystalline COMPOUND 2 may be produced as one or more single crystalline forms of COMPOUND 2.
• the terms "crystalline form”, “single crystalline form” and “polymorph” are synonymous; the terms distinguish between crystals that have different properties (e.g., different XRPD patterns and/or different DSC scan results).
• polymorph includes pseudopolymorphs, which are typically different solvates of a material, and thus their properties differ from one another. Thus, each distinct polymorph and pseudopolymorph of COMPOUND 2 is considered to be a distinct single crystalline form herein.
• substantially crystalline refers to forms that may be at least a particular weight percent crystalline. Particular weight percentages are 10%, 20%, 30%, 40%, 50%, 60%, 70%, 75%, 80%, 85%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, 99.5%, 99.9%, or any percentage between 10% and 100%.
• substantially crystalline refers to a COMPOUND 2 that is at least 70% crystalline.
• substantially crystalline refers to a COMPOUND 2 that is at least 90% crystalline.
• isolated refers to forms that may be at least a particular weight percent of a particular crystalline form of compound . Particular weight percentages are 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, 99.5%, 99.9%, or any percentage between 90% and 100%.
• solvate or solvated means a physical association of a compound, including a crystalline form thereof, of this invention with one or more solvent molecules. This physical association includes hydrogen bonding. In certain instances the solvate will be capable of isolation, for example when one or more solvent molecules are incorporated in the crystal lattice of the crystalline solid. "Solvate or solvated” encompasses both solution-phase and isolable solvates. Representative solvates include, for example, a hydrate, ethanolates or a methanolate.
• hydrate is a solvate wherein the solvent molecule is 3 ⁇ 40 that is present in a defined stoichiometric amount, and may, for example, include hemihydrate, monohydrate, dihydrate, or trihydrate.
• mixture is used to refer to the combined elements of the mixture regardless of the phase- state of the combination (e.g., liquid or liquid/ crystalline).
• seeding is used to refer to the addition of a crystalline material to initiate recrystallization or crystallization.
• antisolvent is used to refer to a solvent in which compounds, including crystalline forms thereof, are poorly soluble.
• pharmaceutically acceptable carrier or adjuvant refers to a carrier or adjuvant that may be administered to a subject, together with a compound of one aspect of this invention, and which does not destroy the pharmacological activity thereof and is nontoxic when administered in doses sufficient to deliver a therapeutic amount of the compound.
• a pharmaceutically-acceptable salt refers to non-toxic acid or base addition salts of the compound to which the term refers. Examples of pharmaceutically acceptable salts are discussed in Berge et ah, 1977, “Pharmaceutically Acceptable Salts.” J. Pharm. Sci. Vol. 66, pp. 1-19.
• the term “about” means approximately, in the region of, roughly, or around. When the term “about” is used in conjunction with a numerical range, it modifies that range by extending the boundaries above and below the numerical values set forth. In general, the term “about” is used herein to modify a numerical value above and below the stated value by a variance of 10%.
• COMPOUND 2 is (5)-N-((5)- l-(2-chlorophenyl)-2-((3,3-difluorocyclobutyl)amino)- 2-oxoethyl)-l-(4-cyanopyridin-2-yl)-N-(5-fluoropyridin-3-yl)-5-oxopyrrolidine-2-carboxamide, a pharmaceutically acceptable salt, solvate, tautomer, stereoisomer, isotopologue, prodrug, or a polymorph thereof.
• COMPOUND 2 has the following chemical structure:
• COMPOUND 2 may also comprise one or more isotopic substitutions.
• isotopic substitutions For example,
• H may be in any isotopic form ("Isotopologues"), including 1 H, 2 H (D or deuterium), and 3 H (T or tritium); C may be in any isotopic form, including 12 C, 13 C, and 14 C; O may be in any isotopic form, including 16 0 and ls O; and the like.
• COMPOUND 2 is enriched in a specific isotopic form of H, C and/or O by at least about 60%, 65%, 70%, 75%, 80%, 85%, 90%, 95%, 96%, 97%, 98%, or 99%.
• COMPOUND 2 in certain embodiments may also be represented in multiple tautomeric forms, in such instances, one aspect of the invention expressly includes all tautomeric forms of COMPOUND 2 described herein, even though only a single tautomeric form may be represented (e.g., keto-enol tautomers). All such isomeric forms of COMPOUND 2 are expressly included herein. Synthesis of COMPOUND 2 is described in US published application US -2013 -0190249- A 1 published July 25, 2013, which is incorporated by reference in its entirety.
• COMPOUND 2 is anionic, or has a functional group which may be anionic (e.g., -NH- may be -N- " ), then a salt may be formed with a suitable cation.
• suitable inorganic cations include, but are not limited to, alkali metal ions such as Na + and K + , alkaline earth cations such as Ca 2+ and Mg 2+ , and other cations such as Al 3+ .
• Examples of some suitable substituted ammonium ions are those derived from: ethylamine, diethylamine, dicyclohexylamine, triethylamine, butylamine, ethylenediamine, ethanolamine, diethanolamine, piperazine, benzylamine, phenylbenzylamine, choline, meglumine, and tromethamine, as well as amino acids, such as lysine and arginine.
• An example of a common quaternary ammonium ion is N(CH 3 ) 4 + .
• COMPOUND 2 is cationic, or has a functional group that may be cationic (e.g., -NHR may be -NH 2 R + ), then a salt may be formed with a suitable anion.
• suitable inorganic anions include, but are not limited to, those derived from the following inorganic acids: hydrochloric, hydrobromic, hydroiodic, sulfuric, sulfurous, nitric, nitrous, phosphoric, and phosphorous.
• Suitable organic anions include, but are not limited to, those derived from the following organic acids: 2-acetyoxybenzoic, acetic, ascorbic, aspartic, benzoic, camphorsulfonic, cinnamic, citric, edetic, ethanedisulfonic, ethanesulfonic, fumaric,
• glucohep tonic gluconic, glutamic, glycolic, hydroxymaleic, hydroxynaphthalene carboxylic, isethionic, lactic, lactobionic, lauric, maleic, malic, methanesulfonic, mucic, oleic, oxalic, palmitic, pamoic, pantothenic, phenylacetic, phenylsulfonic, propionic, pyruvic, salicylic, stearic, succinic, sulfanilic, tartaric, toluenesulfonic, and valeric.
• suitable polymeric organic anions include, but are not limited to, those derived from the following polymeric acids: tannic acid, carboxymethyl cellulose.
• COMPOUND 2 for use in the methods and pharmaceutical compositions provided herein therefore includes COMPOUND 2 itself, as well as its pharmaceutically acceptable salts, solvates, tautomers, stereoisomers, isotopologues, prodrugs or polymorphs.
• COMPOUND 2 provided herein may be modified and converted to a prodrug by appending appropriate functionalities to enhance selected biological properties, e.g., targeting to a particular tissue.
• modifications i.e., prodrugs
• prodrugs include those which increase biological penetration into a given biological compartment (e.g., blood, lymphatic system, central nervous system), increase oral availability, increase solubility to allow administration by injection, alter metabolism and alter rate of excretion.
• prodrugs include esters (e.g., phosphates, amino acid (e.g., valine) esters), carbamates and other pharmaceutically acceptable derivatives, which, upon administration to a subject, are capable of providing active compounds.
• COMPOUND 2 can exist in a variety of solid forms. In one embodiment, provided herein are solid forms that include neat crystal forms. In another embodiment, provided herein are solid forms that include solvated forms and amorphous forms.
• the present disclosure provides certain solid forms of COMPOUND 2. In certain embodiments, the present disclosure provides compositions comprising COMPOUND 2 in a form described herein. In some embodiments of provided compositions, COMPOUND 2 is present as a mixture of one or more solid forms; in some embodiments of provided compositions, COMPOUND 2 is present in a single form.
• COMPOUND 2 is a single crystalline form, or any one of the single crystalline forms described herein. Synthesis of crystalline forms of COMPOUND 2 is described in international application publications WO 2015/138837 and WO 2015/138839, both published September 17, 2015, both incorporated by reference herein in their entireties. Also provided are pharmaceutical compositions comprising at least one pharmaceutically acceptable carrier or diluent; and COMPOUND 2, wherein COMPOUND 2 is a single crystalline form, or any one of the crystalline forms being described herein. Also provided are uses of COMPOUND 2, wherein COMPOUND 2 is a single crystalline form, or any one of the single crystalline forms described herein, to prepare a pharmaceutical composition.
• At least a particular percentage by weight of COMPOUND 2 is crystalline. Particular weight percentages may be 10%, 20%, 30%, 40%, 50%, 60%, 70%, 75%, 80%, 85%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, 99.5%, 99.9%, or any percentage between 10% and 100%.
• a particular percentage by weight of COMPOUND 2 is crystalline
• the remainder of COMPOUND 2 is the amorphous form of COMPOUND 2.
• Non-limiting examples of crystalline COMPOUND 2 include a single crystalline form of compound 1 or a mixture of different single crystalline forms.
• COMPOUND 2 is at least 90% by weight crystalline. In some other
• COMPOUND 2 is at least 95% by weight crystalline.
• COMPOUND 2 is a specific single crystalline form or a combination of single crystalline forms. Particular weight percentages may be 10%, 20%, 30%, 40%, 50%, 60%, 70%, 75%, 80%, 85%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, 99.5%, 99.9%, or any percentage between 10% and 100%.
• COMPOUND 2 is at least 90% by weight of a single crystalline form.
• COMPOUND 2 is at least 95% by weight of a single crystalline form.
• COMPOUND 2 embodiments of the invention may be described with reference to a particular crystalline form of COMPOUND 2, as characterized by one or more properties as discussed herein.
• the descriptions characterizing the crystalline forms may also be used to describe the mixture of different crystalline forms that may be present in a crystalline COMPOUND 2.
• the particular crystalline forms of COMPOUND 2 may also be characterized by one or more of the characteristics of the crystalline form as described herein, with or without regard to referencing a particular crystalline form.
• the crystalline forms are further illustrated by the detailed descriptions and illustrative examples given below.
• the XRPD peaks described in Tables 1 to 2 may vary by + 0.2° depending upon the instrument used to obtain the data.
• the intensity of the XRPD peaks described in Tables 1 to 2 may vary by 10%.
• a single crystalline form, Form 1, of COMPOUND 2 is characterized by the X-ray powder diffraction (XRPD) pattern shown in FIG. 5, and data shown in Table 1, obtained using CuKa radiation.
• the polymorph can be characterized by one or more of the peaks taken from FIG. 5, as shown in Table 1.
• the polymorph can be characterized by one or two or three or four or five or six or seven or eight or nine of the peaks shown in Table 1.
• Form 1 can be characterized by the peaks identified at 2 ⁇ angles of 8.6, 15.6, 18.5, 20.6, 21.6, and 26.4°. In another embodiment, Form 1 can be characterized by the peaks identified at 2 ⁇ angles of 8.6, 15.6, 18.5, and 21.6°.
• Form 1 can be characterized by the differential scanning calorimetry profile (DSC) shown in FIG. 6.
• DSC differential scanning calorimetry profile
• the DSC graph plots the heat flow as a function of temperature from a sample, the temperature rate change being about 10 °C /min.
• the profile is characterized by an endothermic transition with an onset temperature of about 140.1°C with a melt at about 149.9°C.
• Form 1 can be characterized by thermal gravimetric analysis (TGA) shown in FIG. 7.
• TGA thermal gravimetric analysis
• the TGA profile graphs the percent loss of weight of the sample as a function of temperature, the temperature rate change being about 10 °C /min.
• the weight loss represents a loss of about 0.44% of the weight of the sample as the temperature is changed from about 29.0°C to 125.0°C.
• a single crystalline form, Form 2, of the COMPOUND 2 is characterized by the X-ray powder diffraction (XRPD) pattern shown in FIG. 8, and data shown in Table 2, obtained using CuKa radiation.
• the polymorph can be characterized by one or more of the peaks taken from FIG. 8, as shown in Table 2.
• the polymorph can be characterized by one or two or three or four or five or six or seven or eight or nine or ten of the peaks shown in Table 2.
• Form 2 can be characterized by the peaks identified at 2 ⁇ angles of 9.8, 11.6, 19.6, 22.5, 23.0, and 31.4°. In another embodiment, Form 2 can be characterized by the peaks identified at 2 ⁇ angles of 9.8, 11.6, 19.6, and 23.0°.
• Form 2 can be characterized by the differential scanning calorimetry profile (DSC) shown in FIG. 9.
• DSC differential scanning calorimetry profile
• the DSC graph plots the heat flow as a function of temperature from a sample, the temperature rate change being about 10 °C /min.
• the profile is characterized by an endothermic transition with an onset temperature of about 62.7°C with a melt at about 72.5°C, and an endothermic transition with an onset temperature of about 145.6°C with a melt at about 153.6°C.
• Form 2 can be characterized by thermal gravimetric analysis (TGA) shown in FIG. 10.
• TGA thermal gravimetric analysis
• the TGA profile graphs the percent loss of weight of the sample as a function of temperature, the temperature rate change being about 10 °C /min.
• the weight loss represents a loss of about 0.57 % of the weight of the sample as the temperature is changed from about 29.3°C to 170.3°C.
• Other embodiments are directed to a single crystalline form of COMPOUND 2 characterized by a combination of the aforementioned characteristics of any of the single crystalline forms discussed herein.
• the characterization may be by any combination of one or more of the XRPD, TGA, and DSC described for a particular polymorph.
• the single crystalline form of COMPOUND 2 may be characterized by any combination of the XRPD results regarding the position of the major peaks in a XRPD scan; and/or any combination of one or more of parameters derived from data obtained from a XRPD scan.
• the single crystalline form of COMPOUND 2 may also be characterized by TGA determinations of the weight loss associated with a sample over a designated temperature range; and/or the
• the methods provided herein comprise administration or co-administration of one or more DNA demethylating agents.
• the DNA demethylating agents are cytidine analogs.
• the cytidine analog is azacitidine) or 5-aza-2' -deoxycytidine (decitabine).
• the cytidine analog is azacitidine.
• the cytidine analog is 5-aza-2' -deoxycytidine
• the cytidine analog is, for example: ⁇ - ⁇ -D- arabinofuranosylcytosine (Cytarabine or ara-C); pseudoiso-cytidine (psi ICR); 5-fluoro-2'- deoxycytidine (FCdR); 2'-deoxy-2',2'-difluorocytidine (Gemcitabine); 5-aza-2'-deoxy-2' ,2'- difluorocytidine; 5-aza-2'-deoxy-2'-fluorocytidine; l-P-D-ribofuranosyl-2(lH)-pyrimidinone (Zebularine); 2',3'-dideoxy-5-fluoro-3 '-thiacytidine (Emtriva); 2'-cyclocytidine (Ancitabine); 1- P-D-arabinofuranosyl-5-azacytosine (Fazarabine or ara-AC); 6-azacit
• the cytidine analogs include any compound which is structurally related to cytidine or deoxycytidine and functionally mimics and/or antagonizes the action of cytidine or deoxycytidine.
• exemplary cytidine analogs have the structures provided below:
• Cytidine analogs for use in the methods provided herein may be prepared using synthetic methods and procedures referenced herein or otherwise available in the literature. For example, particular methods for synthesizing azacitidine and decitabine are disclosed, e.g. , in U.S. Patent No. 7,038,038 and references discussed therein, each of which is incorporated herein by reference. Other cytidine analogs for use in the methods provided herein may be prepared, e.g. , using procedures known in the art, or may be purchased from a commercial source. In one embodiment, the cytidine analogs for use in the methods provided herein may be prepared in a particular solid form (e.g. , amorphous or crystalline form). See, e.g. , U.S. Patent 6,887,855, issued May 8, 2005 and U.S. Patent 6,943,249, issued September 13, 2005, both of which are incorporated herein by reference in their entireties.
• the cytidine analog used in the methods provided herein is a free base, or a pharmaceutically acceptable salt or solvate thereof.
• the free base or the pharmaceutically acceptable salt or solvate is a solid.
• the free base or the pharmaceutically acceptable salt or solvate is a solid in an amorphous form.
• the free base or the pharmaceutically acceptable salt or solvate is a solid in a crystalline form.
• particular embodiments provide azacitidine and decitabine in solid forms, which can be prepared, for example, according to the methods described in U.S. Patent Nos.
• azacitidine and decitabine in solid forms can be prepared using other methods known in the art.
• cytidine analog used in the methods provided herein is a pharmaceutically acceptable salt of the cytidine analog, which includes, but is not limited to, acetate, adipate, alginate, aspartate, benzoate, benzenesulfonate (besylate), bisulfate, butyrate, citrate, camphorate, camphorsulfonate, cyclopentanepropionate, digluconate, dodecylsulfate, 1,2- ethanedisulfonate (edisylate), ethanesulfonate (esylate), formate, fumarate, glucoheptanoate, glycerophosphate, glycolate, hemisulfate, heptanoate, hexanoate, hydrochloride, hydrobromide, hydroiodide, 2-hydroxyethanesulfonate, lactate, maleate, malonate, methanesul
• Azacitidine is 4-amino- l-P-D-ribofuranozyl-s-triazin-2(lH)-one, also known as VIDAZA® (Celgene Corporation). Its empirical formula is C 8 Hi 2 N 4 0 5 , the molecular weight is 244.
• Azacitidine is a white to off-white solid that is insoluble in acetone, ethanol and methyl ketone; slightly soluble in ethanol/water (50/50), propylene glycol and polyethylene glycol; sparingly soluble in water, water-saturated octanol, 5% dextrose in water, N-methyl-2- pyrrolidone, normal saline and 5% Tween 80 in water, and soluble in dimethylsulfoxide (DMSO).
• DMSO dimethylsulfoxide
• VIDAZA ® is approved for treatment in patients with higher-risk MDS. It is supplied in a sterile form for reconstitution as a suspension for subcutaneous injection or reconstitution as a solution with further dilution for intravenous infusion. Vials of VIDAZA® contain 100 mg of azacitidine and 100 mg of mannitol as a sterile lyophilized powder. The approved dosing schedule is a twice-daily subcutaneous injection or a single daily intravenous infusion on seven consecutive days of a 28-day treatment cycle.
• Oral azacitidine is effective and safe in lower-risk myelodisplastic syndrome (MDS) and acute myeloid leukemia (AML) patients.
• the dose used in MDS and AML patients is 300 mg once daily based on extended dosing (14 or 21 days of the 28-day treatment cycle).
• the starting dose for oral azacitidine is 120 mg and the maximum tolerated dose is 480 mg.
• Decitabine is 4-amino- l-(2-deoxy-P-D-erythro-pentofuranosyl)- l,3,5-triazin- 2(lH)one, also known as DACOGEN ® . Its empirical formula is C 8 Hi 2 N 4 0 4 , the molecular weight is 228.21. Decitabine is a fine, white to almost white powder that is slightly soluble in ethanol/water (50/50), methanol/water (50/50) and methanol; sparingly soluble in water, and soluble in dimethylsulfoxide (DMSO).
• DMSO dimethylsulfoxide
• DACOGEN is approved for treatment in patients with myelodisplastic syndromes. It is supplied in a clear colorless glass vial as white sterile lyophilized powder for injection. Each 20 mL, as a single dose, glass vial contains 50 mg decitabine, 68 mg monobasic potassium phosphate (potassium dihydrogen phosphate) and 11.6 mg sodium hydrochloride.
• compositions and routes of administration are provided.
• a pharmaceutical composition comprising a therapeutically effective amount of a mutant IDHl inhibitor and a DNA demethylating agent.
• the mutant IDHl inhibitor is COMPOUND 2.
• composition comprising a therapeutically effective amount of COMPOUND 2 and azacitidine.
• COMPOUND 2 and azacitidine are formulated as one composition.
• COMPOUND 2 and azacitidine are formulated as separate compositions.
• the compounds utilized in the methods provided herein may be formulated together with a pharmaceutically acceptable carrier or adjuvant into pharmaceutically acceptable compositions prior to be administered to a subject.
• such pharmaceutically acceptable compositions further comprise additional therapeutic agents in amounts effective for achieving a modulation of disease or disease symptoms, including those described herein.
• Pharmaceutically acceptable carriers, adjuvants and vehicles that may be used in the pharmaceutical compositions of one aspect of this invention include, but are not limited to, ion exchangers, alumina, aluminum stearate, lecithin, self-emulsifying drug delivery systems (SEDDS) such as d-oc-tocopherol polyethyleneglycol 1000 succinate, surfactants used in pharmaceutical dosage forms such as Tweens or other similar polymeric delivery matrices, serum proteins, such as human serum albumin, buffer substances such as phosphates, glycine, sorbic acid, potassium sorbate, partial glyceride mixtures of saturated vegetable fatty acids, water, salts or electrolytes, such as protamine sulfate, disodium hydrogen phosphate, potassium hydrogen phosphate, sodium chloride, zinc salts, colloidal silica, magnesium trisilicate, polyvinyl pyrrolidone, cellulose-based substances, polyethylene glycol, sodium
• Cyclodextrins such as ⁇ -, ⁇ -, and ⁇ -cyclodextrin, or chemically modified derivatives such as hydroxyalkylcyclodextrins, including 2- and
• 3-hydroxypropyl-P-cyclodextrins may also be advantageously used to enhance delivery of COMPOUND 2 described herein.
• the pharmaceutical composition comprises COMPOUND 2 and an excipient.
• the pharmaceutical composition that comprises COMPOUND 2 and an excipient is for oral administration.
• the excipient is a diluent, a binder, a disintegrant, a wetting agent, a stabilizer, a glidant, or a lubricant.
• the pharmaceutical composition comprises COMPOUND 2 and/or azacitidine and an excipient.
• the pharmaceutical composition that comprises COMPOUND 2 and/or azacitidine and an excipient is for oral administration.
• Oral delivery formats for COMPOUND 2 and/or azacitidine include, but are not limited to, tablets, capsules, caplets, solutions, suspensions, and syrups, and may also comprise a plurality of granules, beads, powders or pellets that may or may not be encapsulated. Such formats may also be referred to herein as the "drug core" which contains COMPOUND 2 and/or azacitidine.
• the formulation is a tablet comprising COMPOUND 2 and/or azacitidine.
• the formulation is a capsule comprising COMPOUND 2 and/or azacitidine.
• the tablets or capsules provided herein optionally comprise one or more excipients, such as, for example, glidants, diluents, lubricants, colorants, disintegrants, granulating agents, binding agents, polymers, and coating agents.
• the formulation is an immediate release tablet.
• the formulation is a controlled release tablet releasing the active pharmaceutical ingredient (API), e.g. , substantially in the stomach.
• the formulation is a hard gelatin capsule.
• the formulation is a soft gelatin capsule.
• the capsule is a hydroxypropyl methylcellulose (HPMC) capsule.
• the formulation is an immediate release capsule.
• the formulation is an immediate or controlled release capsule releasing the API, e.g. , substantially in the stomach.
• the formulation is a rapidly disintegrating tablet that dissolves substantially in the mouth following administration.
• embodiments herein encompass the use of COMPOUND 2 and/or azacitidine for the preparation of a pharmaceutical composition for treating a malignancy, characterized by the presence of a mutant allele of IDH1, wherein the composition is prepared for oral administration.
• compositions e.g. , immediate release oral formulations and/or formulations that release the API substantially in the stomach
• COMPOUND 2 and/or azacitidine that achieve a particular AUC value (e.g. , AUC(O-t) or AUC(O-co)) in the subject (e.g. , human) to which the formulation is orally administered.
• AUC value e.g. , AUC(O-t) or AUC(O-co)
• Particular embodiments provide oral formulations that achieve an AUC value of at least about 25 ng-hr/mL, at least about 50 ng-hr/mL, at least about 75 ng-hr/mL, at least about 100 ng-hr/mL, at least about 150 ng-hr/mL, at least about 200 ng-hr/mL, at least about 250 ng- hr/mL, at least about 300 ng-hr/mL, at least about 350 ng-hr/mL, at least about 400 ng-hr/mL, at least about 450 ng-hr/mL, at least about 500 ng-hr/mL, at least about 550 ng-hr/mL, at least about 600 ng-hr/mL, at least about 650 ng-hr/mL, at least about 700 ng-hr/mL, at least about 750 ng-hr/mL, at least about 800 ng-hr/mL, at
• compositions e.g. , immediate release oral formulations and/or formulations that release the API substantially in the stomach
• COMPOUND 2 and/or azacitidine that achieve a particular maximum plasma concentration ("Cmax") in the subject to which the formulation is orally administered.
• Particular embodiments provide oral formulations that achieve a Cmax of the COMPOUND 2 and/or citidine analog of at least about 25 ng/mL, at least about 50 ng/mL, at least about 75 ng/mL, at least about 100 ng/mL, at least about 150 ng/mL, at least about 200 ng/mL, at least about 250 ng/mL, at least about 300 ng/mL, at least about 350 ng/mL, at least about 400 ng/mL, at least about 450 ng/mL, at least about 500 ng/mL, at least about 550 ng/mL, at least about 600 ng/mL, at least about 650 ng/mL, at least about 700 ng/mL, at least about 750 ng/mL, at least about 800 ng/mL, at least about 850 ng/mL, at least about 900 ng/mL, at least about 950 ng/mL, at least about 1000 ng/mL, at
• compositions e.g. , immediate release oral formulations and/or formulations that release the API substantially in the stomach
• COMPOUND 2 and/or azacitidine that achieve a particular time to maximum plasma concentration ("Tmax") in the subject to which the formulation is orally administered.
• Particular embodiments provide oral formulations that achieve a Tmax of the cytidine analog of less than about 10 min., less than about 15 min., less than about 20 min., less than about 25 min., less than about 30 min., less than about 35 min., less than about 40 min., less than about 45 min., less than about 50 min., less than about 55 min., less than about 60 min., less than about 65 min., less than about 70 min., less than about 75 min., less than about 80 min., less than about 85 min., less than about 90 min., less than about 95 min., less than about 100 min., less than about 105 min., less than about 110 min., less than about 115 min., less than about 120 min., less than about 130 min., less than about 140 min., less than about 150 min., less than about 160 min., less than about 170 min., less than about 180 min., less than about 190 min., less than about 200 min., less than about 210 min., less than about
• compositions herein provide oral dosage forms comprising COMPOUND 2 and/or azacitidine wherein the oral dosage forms have an enteric coating.
• embodiments provide a permeable or partly permeable (e.g. , "leaky”) enteric coating with pores.
• the permeable or partly permeable enteric-coated tablet releases the COMPOUND 2 and/or azacitidine in an immediate release manner substantially in the stomach.
• dosage forms designed to maximize the absorption and/or efficacious delivery of COMPOUND 2 and/or azacytidine, upon oral administration, e.g. , for release substantially in the stomach.
• certain embodiments herein provide a solid oral dosage form of COMPOUND 2 and/or azacitidine using pharmaceutical excipients designed for immediate release of the API upon oral administration, e.g. , substantially in the stomach.
• Particular immediate release formulations comprise a specific amount of COMPOUND 2 and/or azacitidine and optionally one or more excipients.
• the formulation may be an immediate release tablet or an immediate release capsule (such as, e.g. , an HPMC capsule).
• compositions provided herein comprising COMPOUND 2 and/or azacitidine provided herein (e.g. , immediate release oral formulations and/or formulations that release the API substantially in the stomach).
• azacitidine provided herein
• the formulations provided herein may be prepared using conventional methods known to those skilled in the field of pharmaceutical formulation, as described, e.g. , in pertinent textbooks. See, e.g., REMINGTON, THE SCIENCE AND PRACTICE OF PHARMACY, 20th Edition, Lippincott Williams & Wilkins, (2000); ANSEL et ah, PHARMACEUTICAL DOSAGE FORMS AND DRUG DELIVERY SYSTEMS, 7th Edition, Lippincott Williams & Wilkins, (1999); GIBSON, PHARMACEUTICAL PREFORMULATION AND FORMULATION, CRC Press (2001).
• formulations provided herein comprise COMPOUND 2 and/or azacitidine in a specific amount.
• the specific amount of COMPOUND 2 and/or azacitidine in the formulation is, e.g. , about 10 mg.
• the specific amount is about 20 mg.
• the specific amount is about 40 mg.
• the specific amount is about 60 mg.
• the specific amount is about 80 mg.
• the specific amount is about 100 mg.
• the specific amount is about 120 mg.
• the specific amount is about 140 mg.
• the specific amount is about 160 mg. In one embodiment, the specific amount is about 180 mg. In one embodiment, the specific amount is about 200 mg. In one embodiment, the specific amount is about 220 mg. In one embodiment, the specific amount is about 240 mg. In one embodiment, the specific amount is about 260 mg. In one embodiment, the specific amount is about 280 mg. In one embodiment, the specific amount is about 300 mg. In one embodiment, the specific amount is about 320 mg. In one embodiment, the specific amount is about 340 mg. In one embodiment, the specific amount is about 360 mg. In one embodiment, the specific amount is about 380 mg. In one embodiment, the specific amount is about 400 mg. In one embodiment, the specific amount is about 420 mg. In one embodiment, the specific amount is about 440 mg.
• the specific amount is about 460 mg. In one embodiment, the specific amount is about 480 mg. In one embodiment, the specific amount is about 500 mg. In one embodiment, the specific amount is about 600 mg. In one embodiment, the specific amount is about 700 mg. In one embodiment, the specific amount is about 800 mg. In one embodiment, the specific amount is about 900 mg. In one embodiment, the specific amount is about 1000 mg. In one embodiment, the specific amount is about 1100 mg. In one embodiment, the specific amount is about 1200 mg. In one embodiment, the specific amount is about 1300 mg. In one embodiment, the specific amount is about 1400 mg. In one embodiment, the specific amount is about 1500 mg. In one embodiment, the specific amount is about 1600 mg. In one embodiment, the specific amount is about 1700 mg.
• the specific amount is about 1800 mg. In one embodiment, the specific amount is about 1900 mg. In one embodiment, the specific amount is about 2000 mg. In one embodiment, the specific amount is about 2100 mg. In one embodiment, the specific amount is about 2200 mg. In one embodiment, the specific amount is about 2300 mg. In one embodiment, the specific amount is about 2400 mg. In one embodiment, the specific amount is about 2500 mg. In one embodiment, the specific amount is about 3000 mg. In one embodiment, the specific amount is about 4000 mg. In one embodiment, the specific amount is about 5000 mg.
• the formulation is a tablet, wherein the tablet is
• the method for forming the tablets is direct compression of a powdered, crystalline and/or granular composition comprising COMPOUND 2 and/or azacitidine alone or in combination with one or more excipients, such as, for example, carriers, additives, polymers, or the like.
• the tablets may be prepared using wet granulation or dry granulation processes.
• the tablets are molded rather than compressed, starting with a moist or otherwise tractable material.
• compression and granulation techniques are used.
• the formulation is a capsule, wherein the capsules may be manufactured using standard, art-recognized capsule processing procedures and equipments.
• soft gelatin capsules may be prepared in which the capsules contain a mixture of COMPOUND 2 and/or the cytidine analog and vegetable oil or non-aqueous, water miscible materials such as, for example, polyethylene glycol and the like.
• hard gelatin capsules may be prepared containing granules of COMPOUND 2 and/or the cytidine analog in combination with a solid pulverulent carrier, such as, for example, lactose, saccharose, sorbitol, mannitol, potato starch, corn starch, amylopectin, cellulose derivatives, or gelatin.
• a hard gelatin capsule shell may be prepared from a capsule composition comprising gelatin and a small amount of plasticizer such as glycerol.
• the capsule shell may be made of a carbohydrate material.
• the capsule composition may additionally include polymers, colorings, flavorings and opacifiers as required.
• the capsule comprises HPMC.
• the formulation of COMPOUND 2 and/or azacitidine is prepared using aqueous solvents without causing significant hydrolytic degradation ofazacitidine.
• the formulation of COMPOUND 2 and/or azacitidine is a tablet which contains a coating applied to the drug core using aqueous solvents without causing significant hydrolytic degradation of azacitidine in the formulation.
• water is employed as the solvent for coating the drug core.
• the oral dosage form of COMPOUND 2 and/or azacitidine is a tablet containing a film coat applied to the drug core using aqueous solvents.
• water is employed as the solvent for film-coating.
• the tablet containing COMPOUND 2 and/or azacitidine is film-coated using aqueous solvents without effecting degradation of the pharmaceutical composition.
• water is used as the film coating solvent without effecting degradation of the pharmaceutical composition.
• an oral dosage form comprising COMPOUND 2 and/or azacitidine and an aqueous film coating effects immediate drug release upon oral delivery.
• the oral dosage form comprising COMPOUND 2 and/or azacitidine and an aqueous film coating effects controlled drug release to the upper gastrointestinal tract, e.g. , the stomach, upon oral administration.
• a tablet with an aqueous-based film coating comprises COMPOUND 2 and/or azacytidine as the API.
• a controlled release pharmaceutical formulation for oral administration of azacitidine that releases COMPOUND 2 and/or azacitidine substantially in the stomach comprising: a) a specific amount of COMPOUND 2 and/or azacitidine; b) a drug release controlling component for controlling the release of COMPOUND 2 and/or azacitidine substantially in the upper gastrointestinal tract, e.g. , the stomach; and c) optionally one or more excipients.
• the oral dosage form comprising COMPOUND 2 and/or azacitidine is prepared as a controlled release tablet or capsule which includes a drug core comprising the pharmaceutical composition and optional excipients.
• a formulation provided herein comprising COMPOUND 2 and/or azacitidine provided herein is a controlled release tablet or capsule, which comprises a therapeutically effective amount of COMPOUND 2 and/or azacitidine, a drug release controlling component that controls the release of COMPOUND 2 and/or azacitidine substantially in the stomach upon oral administration, and optionally, one or more excipients.
• a drug release controlling component that is a polymer matrix, which swells upon exposure to gastric fluid to effect the gastric retention of the formulation and the sustained release of COMPOUND 2 and/or azacitidine from the polymer matrix substantially in the stomach.
• such formulations may be prepared by incorporating COMPOUND 2 and/or azacitidine into a suitable polymeric matrix during formulation.
• suitable polymeric matrix examples of such formulations are known in the art. See, e.g., Shell et al, U.S. Patent Publication No. 2002/0051820 (Application No. 09/990,061); Shell et al, U.S. Patent Publication No. 2003/0039688 (Application No. 10/045,823); Gusler et al, U.S. Patent
• the drug release controlling component may comprise a shell surrounding the drug-containing core, wherein the shell releases COMPOUND 2 and/or azacitidine from the core by, e.g., permitting diffusion of COMPOUND 2 and/or azacitidine from the core and promoting gastric retention of the formulation by swelling upon exposure to gastric fluids to a size that is retained in the stomach.
• such formulations may be prepared by first compressing a mixture of COMPOUND 2 and/or azacitidine and one or more excipients to form a drug core, and compressing another powdered mixture over the drug core to form the shell, or enclosing the drug core with a capsule shell made of suitable materials. Examples of such formulations are known in the art. See, e.g., Berner et al, U.S. Patent
• the pharmaceutical formulations provided herein contain COMPOUND 2 and/or azacitidine and, optionally, one or more excipients to form a "drug core.”
• excipients include, e.g., diluents (bulking agents), lubricants, disintegrants, fillers, stabilizers, surfactants, preservatives, coloring agents, flavoring agents, binding agents, excipient supports, glidants, permeation enhancement excipients, plasticizers and the like, e.g., as known in the art. It will be understood by those in the art that some substances serve more than one purpose in a pharmaceutical composition.
• some substances are binders that help hold a tablet together after compression, yet are also disintegrants that help break the tablet apart once it reaches the target delivery site. Selection of excipients and amounts to use may be readily determined by the formulation scientist based upon experience and consideration of standard procedures and reference works available in the art.
• formulations provided herein comprise one or more binders.
• Binders may be used, e.g. , to impart cohesive qualities to a tablet, and thus ensure that the tablet remains intact after compression.
• Suitable binders include, but are not limited to, starch
• Binding agents also include, e.g.
• acacia agar, alginic acid, cabomers, carrageenan, cellulose acetate phthalate, ceratonia, chitosan, confectioner's sugar, copovidone, dextrates, dextrin, dextrose, ethylcellulose, gelatin, glyceryl behenate, guar gum, hydroxyethyl cellulose, hydroxyethylmethyl cellulose, hydroxypropyl cellulose, hydroxypropyl starch, hypromellose, inulin, lactose, magnesium aluminum silicate, maltodextrin, maltose,
• the binding agent can be, relative to the drug core, in the amount of about 2% w/w of the drug core; about 4% w/w of the drug core, about 6% w/w of the drug core, about 8% w/w of the drug core, about 10% w/w of the drug core, about 12% w/w of the drug core, about 14% w/w of the drug core, about 16% w/w of the drug core, about 18% w/w of the drug core, about 20% w/w of the drug core, about 22% w/w of the drug core, about 24% w/w of the drug core, about 26% w/w of the drug core, about 28% w/w of the drug core, about 30% w/w of the drug core, about 32% w/w of the drug core, about 34% w/w/
• formulations provided herein comprise one or more diluents.
• Diluents may be used, e.g. , to increase bulk so that a practical size tablet is ultimately provided.
• Suitable diluents include dicalcium phosphate, calcium sulfate, lactose, cellulose, kaolin, mannitol, sodium chloride, dry starch, microcrystalline cellulose (e.g.
• micro fine cellulose pregelitinized starch, calcium carbonate, calcium sulfate, sugar, dextrates, dextrin, dextrose, dibasic calcium phosphate dihydrate, tribasic calcium phosphate, kaolin, magnesium carbonate, magnesium oxide, maltodextrin, mannitol, polymethacrylates (e.g. , EUDRAGIT), potassium chloride, sodium chloride, sorbitol and talc, among others.
• Diluents also include, e.g.
• Diluents may be used in amounts calculated to obtain a desired volume for a tablet or capsule; in certain embodiments, a diluent is used in an amount of about 5% or more, about 10% or more, about 15% or more, about 20% or more, about 22% or more, about 24% or more, about 26% or more, about 28% or more, about 30% or more, about 32% or more, about 34% or more, about 36% or more, about 38% or more, about 40% or more, about 42% or more, about 44% or more, about 46% or more, about 48% or more, about 50% or more, about 52% or more, about 54% or more, about 56% or more, about 58% or more, about 60% or more, about 62% or more, about 64% or more, about 68% or more, about 70% ore more,
• formulations provided herein comprise one or more lubricants.
• Lubricants may be used, e.g. , to facilitate tablet manufacture; examples of suitable lubricants include, for example, vegetable oils such as peanut oil, cottonseed oil, sesame oil, olive oil, corn oil, and oil of theobroma, glycerin, magnesium stearate, calcium stearate, and stearic acid. In certain embodiments, stearates, if present, represent no more than approximately 2 weight % of the drug-containing core. Further examples of lubricants include, e.g.
• the lubricant is magnesium stearate.
• the lubricant is present, relative to the drug core, in an amount of about 0.2% w/w of the drug core, about 0.4% w/w of the drug core, about 0.6% w/w of the drug core, about 0.8% w/w of the drug core, about 1.0% w/w of the drug core, about 1.2% w/w of the drug core, about 1.4% w/w of the drug core, about 1.6% w/w of the drug core, about 1.8% w/w of the drug core, about 2.0% w/w of the drug core, about 2.2% w/w of the drug core, about 2.4% w/w of the drug core, about 2.6% w/w of the drug core, about 2.8% w/w of the drug core, about 3.0% w/w of the drug core, about 3.5% w/w of the drug core, about 4% w/w of the drug core, about 4.5% w/w of the drug core, about 5% w/w of the drug core, about 6% w/w of
• formulations provided herein comprise one or more disintegrants.
• Disintegrants may be used, e.g. , to facilitate disintegration of the tablet, and may be, e.g. , starches, clays, celluloses, algins, gums or crosslinked polymers.
• Disintegrants also include, e.g. , alginic acid, carboxymethylcellulose calcium, carboxymethylcellulose sodium (e.g. , AC-DI-SOL, PRIMELLOSE), colloidal silicon dioxide, croscarmellose sodium, crospovidone (e.g.
• guar gum magnesium aluminum silicate, methyl cellulose, microcrystalline cellulose, polacrilin potassium, powdered cellulose, pregelatinized starch, sodium alginate, sodium starch glycolate (e.g. , EXPLOTAB) and starch.
• Additional disintegrants include, e.g. , calcium alginate, chitosan, sodium docusate,
• the disintegrant is, relative to the drug core, present in the amount of about 1% w/w of the drug core, about 2% w/w of the drug core, about 3% w/w of the drug core, about 4% w/w of the drug core, about 5% w/w of the drug core, about 6% w/w of the drug core, about 7% w/w of the drug core, about 8% w/w of the drug core, about 9% w/w of the drug core, about 10% w/w of the drug core, about 12% w/w of the drug core, about 14% w/w of the drug core, about 16% w/w of the drug core, about 18% w/w of the drug core, about 20% w/w of the drug core, about 22% w/w of the drug core, about 24% w/w of the drug core, about 26% w/w of the drug core, about 28% w//
• formulations provided herein comprise one or more stabilizers.
• Stabilizers also called absorption enhancers
• Stabilizing agents include, e.g.
• d-Alpha-tocopheryl polyethylene glycol 1000 succinate (Vitamin E TPGS), acacia, albumin, alginic acid, aluminum stearate, ammonium alginate, ascorbic acid, ascorbyl palmitate, bentonite, butylated hydroxytoluene, calcium alginate, calcium stearate, calcium carboxymethylcellulose, carrageenan, ceratonia, colloidal silicon dioxide, cyclodextrins, diethanolamine, edetates, ethylcellulose, ethyleneglycol palmitostearate, glycerin monostearate, guar gum, hydroxypropyl cellulose, hypromellose, invert sugar, lecithin, magnesium aluminum silicate, monoethanolamine, pectin, poloxamer, polyvinyl alcohol, potassium alginate, potassium polacrilin, povidone, propyl gallate, propylene glycol, propylene glycol alginate,
• the stabilizer is, relative to the drug core, present in the amount of about 1% w/w of the drug core, about 2% w/w of the drug core, about 3% w/w of the drug core, about 4% w/w of the drug core, about 5% w/w of the drug core, about 6% w/w of the drug core, about 7% w/w of the drug core, about 8% w/w of the drug core, about 9% w/w of the drug core, about 10% w/w of the drug core, about 12% w/w of the drug core, about 14% w/w of the drug core, about 16% w/w of the drug core, about 18% w/w of the drug core, about 20% w/w of the drug core, about 22% w/w of the drug core, about 24% w/w of the drug core, about 26% w/w of the drug core, about 28% w/w of the drug core, about 30% w/w of the
• formulations provided herein comprise one or more glidants.
• Glidants may be used, e.g. , to improve the flow properties of a powder composition or granulate or to improve the accuracy of dosing.
• Excipients that may function as glidants include, e.g. , colloidal silicon dioxide, magnesium trisilicate, powdered cellulose, starch, tribasic calcium phosphate, calcium silicate, powdered cellulose, colloidal silicon dioxide, magnesium silicate, magnesium trisilicate, silicon dioxide, starch, tribasic calcium phosphate, and talc.
• the glidant is, relative to the drug core, present in the amount of less than about 1% w/w of the drug core, about 1% w/w of the drug core, about 2% w/w of the drug core, about 3% w/w of the drug core, about 4% w/w of the drug core, about 5% w/w of the drug core, about 6% w/w of the drug core, about 7% w/w of the drug core, about 8% w/w of the drug core, about 9% w/w of the drug core, about 10% w/w of the drug core, about 12% w/w of the drug core, about 14% w/w of the drug core, about 16% w/w of the drug core, about 18% w/w of the drug core, about 20% w/w of the drug core, about 22% w/w of the drug core, about 24% w/w of the drug core, about 26% w/w of the drug core, about 28% w/w of
• formulations provided herein comprise one or more permeation enhancers (also called, e.g. , permeability enhancers).
• the permeation enhancer enhances the uptake of azacitidine through the gastrointestinal wall (e.g. , the stomach).
• the permeation enhancer alters the rate and/or amount of azacitidine that enters the bloodstream.
• d-alpha-tocopheryl polyethylene glycol- 1000 succinate (Vitamin E TPGS) is used as a permeation enhancer.
• one or more other suitable permeation enhancers are used, including, e.g. , any permeation enhancer known in the art.
• the pharmaceutical compositions provided herein may be administered orally, parenterally, by inhalation spray, topically, rectally, nasally, buccally, vaginally or via an implanted reservoir, preferably by oral administration or administration by injection.
• the pharmaceutical compositions may contain any conventional non-toxic pharmaceutically-acceptable carriers, adjuvants or vehicles.
• the pH of the formulation may be adjusted with pharmaceutically acceptable acids, bases or buffers to enhance the stability of the formulated compound or its delivery form.
• parenteral as used herein includes subcutaneous, intracutaneous, intravenous, intramuscular, intraarticular, intraarterial, intrasynovial, intrasternal, intrathecal, intralesional and intracranial injection or infusion techniques.
• the pharmaceutical compositions provided herein may be in the form of a sterile injectable preparation, for example, as a sterile injectable aqueous or oleaginous suspension.
• This suspension may be formulated according to techniques known in the art using suitable dispersing or wetting agents (such as, for example, Tween 80) and suspending agents.
• the sterile injectable preparation may also be a sterile injectable solution or suspension in a non-toxic parenterally acceptable diluent or solvent, for example, as a solution in 1,3-butanediol.
• suitable vehicles and solvents that may be employed are mannitol, water, Ringer' s solution and isotonic sodium chloride solution.
• sterile, fixed oils are conventionally employed as a solvent or suspending medium.
• any bland fixed oil may be employed including synthetic mono- or diglycerides.
• Fatty acids, such as oleic acid and its glyceride derivatives are useful in the preparation of injectables, as are natural pharmaceutically- acceptable oils, such as olive oil or castor oil, especially in their polyoxyethylated versions.
• These oil solutions or suspensions may also contain a long-chain alcohol diluent or dispersant, or carboxymethyl cellulose or similar dispersing agents which are commonly used in the formulation of pharmaceutically acceptable dosage forms such as emulsions and or suspensions.
• Other commonly used surfactants such as Tweens or Spans and/or other similar emulsifying agents or bioavailability enhancers which are commonly used in the manufacture of
• compositions may also be used for the purposes of formulation.
• compositions provided herein may also be administered in the form of suppositories for rectal administration.
• suppositories for rectal administration.
• These compositions can be prepared by mixing a compound of one aspect of this invention with a suitable non-irritating excipient which is solid at room temperature but liquid at the rectal temperature and therefore will melt in the rectum to release the active components.
• suitable non-irritating excipient include, but are not limited to, cocoa butter, beeswax and polyethylene glycols.
• Topical administration of the pharmaceutical compositions provided herein is useful when the desired treatment involves areas or organs readily accessible by topical application.
• the pharmaceutical composition should be formulated with a suitable ointment containing the active components suspended or dissolved in a carrier.
• Carriers for topical administration of the compounds of one aspect of this invention include, but are not limited to, mineral oil, liquid petroleum, white petroleum, propylene glycol, polyoxyethylene polyoxypropylene compound, emulsifying wax and water.
• the pharmaceutical composition can be formulated with a suitable lotion or cream containing the active compound suspended or dissolved in a carrier with suitable emulsifying agents.
• Suitable carriers include, but are not limited to, mineral oil, sorbitan monostearate, polysorbate 60, cetyl esters wax, cetearyl alcohol, 2-octyldodecanol, benzyl alcohol and water.
• the pharmaceutical compositions provided herein may also be topically applied to the lower intestinal tract by rectal suppository formulation or in a suitable enema formulation. Topically-transdermal patches are also included herein.
• compositions provided herein may be administered by nasal aerosol or inhalation.
• Such compositions are prepared according to techniques well-known in the art of pharmaceutical formulation and may be prepared as solutions in saline, employing benzyl alcohol or other suitable preservatives, absorption promoters to enhance bioavailability, fluorocarbons, and/or other solubilizing or dispersing agents known in the art.
• compositions provided herein comprise a combination of COMPOUND 2 and azacitidine
• both the COMPOUND 2 and azacitidine should be present at dosage levels of between about 1 to 100%, and more preferably between about 5 to 95% of the dosage normally administered in a monotherapy regimen.
• Azacitidine may be administered separately, as part of a multiple dose regimen, from the compounds of one aspect of this invention.
• azacitidine may be part of a single dosage form, mixed together with COMPOUND 2 in a single composition.
• the compositions provided herein can, for example, be administered by injection, intravenously, intraarterially, subdermally, intraperitoneally, intramuscularly, or subcutaneously; or orally, buccally, nasally, transmucosally, topically, in an ophthalmic preparation, or by inhalation, with a dosage ranging from about 0.5 to about 100 mg/kg of body weight, alternatively dosages between 1 mg and 1000 mg/dose, every 4 to 120 hours, or according to the requirements of the particular drug.
• the methods herein contemplate administration of an effective amount of compound or compound composition to achieve the desired or stated effect.
• the pharmaceutical compositions are administered from about 1 to about 6 times per day or alternatively, as a continuous infusion.
• Such administration can be used as a chronic or acute therapy.
• the amount of active ingredient that may be combined with the carrier materials to produce a single dosage form varies depending upon the host treated and the particular mode of administration.
• a typical preparation contains from about 5% to about 95% active compound (w/w).
• such preparations contain from about 20% to about 80% active compound.
• a maintenance dose of a compound, composition or combination provided herein may be administered, if necessary. Subsequently, the dosage or frequency of administration, or both, may be reduced, as a function of the symptoms, to a level at which the improved condition is retained when the symptoms have been alleviated to the desired level. Subjects may, however, require intermittent treatment on a long-term basis upon any recurrence of disease symptoms.
• COMPOUND 2 is administered in compositions, comprising COMPOUND 2, and one or more polymer(s) as part of a solid dispersion (e.g. , an amorphous solid dispersion).
• the solid dispersion comprises COMPOUND 2, and one or more polymer(s).
• the solid dispersion comprises COMPOUND 2, one or more polymer(s), and one or more surfactant(s).
• the solid dispersion comprises COMPOUND 2, and one polymer.
• the solid dispersion comprises COMPOUND 2, one polymer, and a surfactant.
• the solid dispersion comprises
• COMPOUND 2 one or more polymer(s), and optionally one or more solubility enhancing surfactant.
• the aqueous solubility of Form 1 is about 0.025 mg/mL to about 0.035 mg/mL and the aqueous solubility of Form 2 is about 0.008 mg/mL to about 0.010 mg/mL.
• Form 2 has a solubility of about 0.018 mg/mL in fasted state simulated intestinal fluid (FASSIF) at a pH of 6.1 at 4 hours.
• amorphous spray-dried dispersions have a solubility of about 0.05 mg/mL to about 0.50 mg/mL in FASSIF at 3 hours.
• the solid dispersion exhibits at least about 20%, at least about 30%, at least about 40%, at least about 50%, at least about 60%, at least about 70%, at least about 80%, or at least about 90% higher exposure of COMPOUND 2, when administered to a subject as compared to administration of in-situ amorphous COMPOUND 2. In some embodiments, the solid dispersion exhibits at least about 20%, at least about 30%, at least about 40%, at least about 50%, at least about 60%, at least about 70%, at least about 80%, or at least about 90% higher exposure of COMPOUND 2, when administered to a subject as compared to administration of neat crystalline COMPOUND 2.
• COMPOUND 2 exposure is improved when solid dispersion dosage forms are administered as compared to neat crystalline COMPOUND 2 Form 2.
• At least a portion of COMPOUND 2, in the solid dispersion is in the amorphous state (e.g. , at least about 50%, at least about 55%, at least about 60%, at least about 65%, at least about 70%, at least about 75%, at least about 80%, at least about 85%, at least about 90%, at least about 95%, at least about 98%, or at least about 99%).
• the solid dispersion is substantially free of crystalline COMPOUND 2.
• the composition is an amorphous solid (e.g. spray dried) dispersion comprising COMPOUND 2, and a polymer.
• the amorphous solid dispersion can include, e.g. , less than about 30%, less than about 20%, less than about 15%, less than about 10%, less than about 5%, less than about 4%, less than about 3%, less than about 2%, or less than about 1% of crystalline COMPOUND 2, e.g. , be substantially free of crystalline COMPOUND 2.
• the solid dispersion exhibits a predetermined level of physical and/or chemical stability.
• the solid dispersion retains about 50%, about 60%, about 70%, about 80%, about 90%, about 95%, about 98%, or about 99%, of amorphous COMPOUND 2, when stored at 25°C in a closed water tight container, e.g. , an amber glass vial, high density polyethylene (HDPE) container or double polyethylene bags with twisted nylon tie placed in an HDPE container with desiccant.
• a closed water tight container e.g. , an amber glass vial, high density polyethylene (HDPE) container or double polyethylene bags with twisted nylon tie placed in an HDPE container with desiccant.
• HDPE high density polyethylene
• the polymer increases the chemical or physical stability (e.g. , as measured by a Modulated Differential Scanning Calorimeter) of COMPOUND 2, when stored (e.g. , at 2-8°C, e.g. 4°C or at room temperature) by at least about 10% (e.g. , by at least about 20%, by at least about 30%, by at least about 40%, by at least about 50%, by at least about 60%, by at least about 70%, by at least about 80%, or by at least about 90%) compared to amorphous COMPOUND 2, without being in the presence of the polymer.
• a Modulated Differential Scanning Calorimeter e.g. , as measured by a Modulated Differential Scanning Calorimeter
• a solid dispersion generally exhibits a glass transition temperature, where the dispersion makes a transition from a glassy solid to a rubbery composition.
• the higher the glass transition temperature the greater the physical stability of the dispersion.
• the existence of a glass transition temperature generally indicates that at least a large portion of the composition (e.g. , dispersion) is in an amorphous state.
• the glass transition temperature (Tg) of a solid dispersion suitable for pharmaceutical applications is generally at least about 50°C. In some embodiments, higher temperatures are preferred. Therefore, in some embodiments, a solid dispersion disclosed herein has a Tg of at least about 100°C (e.g.
• the Tg is up to about 200°C. In some embodiments, the Tg is up to about 130°C (e.g.
• the glass transition temperatures disclosed herein are measured under dry conditions.
• the solid dispersion has a higher glass transition temperature than the glass transition temperature of amorphous COMPOUND 2, without being in the presence of the polymer(s). In some embodiments, the solid dispersion has a relaxation rate that is lower than the relaxation rate of amorphous COMPOUND 2, without being in the presence of the polymer(s).
• polymers in the solid dispersion include cellulose derivatives (e.g. , hydroxypropylmethylcellulose also known as hypromellose, (HPMC),
• HPMC hypromellose
• hydroxypropylmethylcellulose phthalate also known as hypromellose phthalate (HPMCP), hydroxypropylmethylcellulose acetate succinate, also known as hpromellose acetate succinate, (HPMCAS), hydroxypropylcellulose (HPC)), ethylcellulose, or cellulose acetate phthalate;
• polyvinylpyrrolidones PVP
• polyethylene glycols PEG
• polyvinyl alcohols PVA
• polyvinyl esters such as Polyvinyl Acetate Phthalate (PVAP)
• acrylates such as polymethacrylate (e.g. , Eudragit.RTM. E); cyclodextrins (e.g.
• PVP-VA polyvinylpyrollidone-vinyl acetate
• PVMA Polyvinyl caprolactam-poly vinyl
• acetate-polyethyleneglycol copolymer Methylacrylate/methacrylic acid copolymer
• Soluplus Copovidone
• mixtures thereof including for example polyvinylpyrollidone-vinyl acetate (PVP-VA), Polyvinyl caprolactam-poly vinyl, and acetate-polyethyleneglycol copolymer, Methylacrylate/methacrylic acid copolymer; Soluplus; Copovidone; and mixtures thereof.
• the solid dispersion includes one water-soluble polymer. In some embodiments, the solid dispersion includes one partially water-soluble polymer. In some embodiments, the polymer is a cellulose polymer.
• the polymer is HPMCAS (e.g. , HPMCAS of different grades: HPMCAS-M, HPMCAS-MG or HPMCAS -HG).
• the polymer is PVAP.
• the polymer is HPMC (e.g. , HPMC of different grades: HMPC60SH50, HPMCE50 or HPMCE15).
• the polymer is HPMCP (e.g. , HPMCP of different grades: e.g. , HMPCP-HP55).
• the polymer is a pH-dependent enteric polymer.
• pH-dependent enteric polymers include, but are not limited to, cellulose derivatives (e.g. , cellulose acetate phthalate (CAP)), HPMCP, HPMCAS, carboxymethylcellulose (CMC) or a salt thereof (e.g. , a sodium salt such as (CMC-Na)); cellulose acetate trimellitate (CAT),
• HPCAP hydroxypropylcellulose acetate phthalate
• HPMCAP hydroxypropylmethyl-cellulose acetate phthalate
• MCAP methylcellulose acetate phthalate
• polymethacrylates e.g. , Eudragit S
• the polymer is hydroxypropylmethylcellulose acetate succinate, also known as hypromellose acetate succinate, (HPMCAS), e.g. , HMPCAS-HG.
• HPMCAS hypromellose acetate succinate
• the polymer(s) is an insoluble cross-linked polymer, for example a polyvinylpyrrolidone (e.g. , Crospovidone).
• the polymer(s) is polyvinylpyrrolidone (PVP).
• the one or more polymer(s) is present in the solid dispersion in an amount of between about 10% w/w and 90% w/w (e.g. , between about 20% w/w and about 80% w/w; between about 30% w/w and about 70% w/w; between about 40% w/w and about 60% w/w; or between about 15% w/w and about 35% w/w).
• the polymer(s) is present in the solid dispersion in an amount of from about 10% w/w to about 80% w/w, for example from about 30% w/w to about 75% w/w, or from about 40% w/w to about 65% w/w, or from about 45% w/w to about 55% w/w, for example, about 46% w/w, about 47% w/w, about 48% w/w, about 49% w/w, about 50% w/w, about 51% w/w, about 52% w/w, about 53% w/w, or about 54% w/w.
• the polymer(s) is present in the solid dispersion in an amount of about 48% w/w, about 48.5% w/w, about 49% w/w, about 49.5% w/w, about 50% w/w, about 50.5% w/w, about 51% w/w, about 51.5% w/w, about 52% w/w, or about 52.5% w/w.
• the polymer(s) is present in the solid dispersion in an amount of from about 30% w/w to about 70% w/w. In some embodiments, the polymer(s) is present in the solid dispersion in an amount of from about 35% w/w to about 65% w/w. In some embodiments, the polymer(s) is present in the solid dispersion in an amount of from about 40% w/w to about 60% w/w. In some embodiments, the polymer(s) is present in the solid dispersion in an amount of from about 45% w/w to about 55% w/w. In some embodiments, the polymer(s) is present in the solid dispersion in an amount of about 50% w/w.
• COMPOUND 2 is present in the solid dispersion in an amount of from about 10% w/w and 90% w/w (e.g. , between about 20% w/w and about 80% w/w; between about 30% w/w and about 70% w/w; between about 40% w/w and about 60% w/w; or between about 15% w/w and about 35% w/w).
• COMPOUND 2 is present in the solid dispersion in an amount of from about 10% w/w to about 80% w/w, for example from about 30% w/w to about 75% w/w, or from about 40% w/w to about 65% w/w, or from about 45% w/w to about 55% w/w, for example, about 46% w/w, about 47% w/w, about 48% w/w, about 49% w/w, about 50% w/w, about 51% w/w, about 52% w/w, about 53% w/w, or about 54% w/w.
• COMPOUND 2 is present in the solid dispersion in an amount of about 48% w/w, about 48.5% w/w, about 49% w/w, about 49.5% w/w, about 50% w/w, about 50.5% w/w, about 51% w/w, about 51.5% w/w, about 52% w/w, or about 52.5% w/w.
• COMPOUND 2 is present in the solid dispersion in an amount of from about 30% w/w to about 70% w/w. In some embodiments, COMPOUND 2, is present in the solid dispersion in an amount of from about 35% w/w to about 65% w/w. In some embodiments, COMPOUND 2, is present in the solid dispersion in an amount of from about 40% w/w to about 60% w/w. In some embodiments, COMPOUND 2, is present in the solid dispersion in an amount of from about 45% w/w to about 55% w/w. In some embodiments, COMPOUND 2, is present in the solid dispersion in an amount of about 50% w/w.
• the solid dispersion includes about 20% w/w to about 80% w/w COMPOUND 2, and about 20% w/w to about 80% of polymer(s). In another embodiment, the solid dispersion includes about 25% w/w to about 75% w/w COMPOUND 2, and about 25% w/w to about 75% of polymer(s). In another embodiment, the solid dispersion includes about 30% w/w to about 70% w/w COMPOUND 2, and about 30% w/w to about 70% of polymer(s). In another embodiment, the solid dispersion includes about 35% w/w to about 65% w/w
• the solid dispersion includes about 40% w/w to about 60% w/w COMPOUND 2, and about 40% w/w to about 60% of polymer(s). In another embodiment, the solid dispersion includes about 45% w/w to about 55% w/w COMPOUND 2, and about 45% w/w to about 55% of polymer(s). In another embodiment, the solid dispersion includes about 50% w/w COMPOUND 2, and about 50% w/w of polymer(s).
• the solid dispersion includes about 45% w/w to about 55% w/w COMPOUND 2, and about 45% w/w to about 55% w/w HPMCAS (e.g. , HPMCAS-MG or HPMCAS-HG, or other grades such as LF, MF, HF, or LG) or PVAP.
• the solid dispersion includes about 50% w/w COMPOUND 2, and about 50% w/w of HPMCAS.
• the solid dispersion also includes a surfactant or inert pharmaceutically acceptable substance.
• surfactants in the solid dispersion include sodium lauryl sulfate (SLS), vitamin E or a derivative thereof (e.g. , vitamin E TPGS), Docusate Sodium, sodium dodecyl sulfate, polysorbates (such as Tween 20 and Tween 80), poloxamers (such as Poloxamer 335 and Poloxamer 407), glyceryl monooleate, Span 65, Span 25, Capryol 90, pluronic copolymers (e.g. , Pluronic F108, Pluronic P- 123), and mixtures thereof.
• the surfactant is SLS.
• the surfactant is vitamin E or a derivative thereof (e.g. , vitamin E TPGS).
• the surfactant is present in the solid dispersion in an amount of from about 0.1% w/w to about 10% w/w, for example from about 0.5% w/w to about 2% w/w, or from about 1% w/w to about 3% w/w, from about 1% w/w to about 4% w/w, or from about 1% w/w to about 5% w/w.
• the surfactant is present in the solid dispersion in an amount of about 0.1% w/w, about 0.2% w/w, about 0.3% w/w, about 0.4%w/w, about 0.5% w/w, about 0.6% w/w, about 0.7% w/w, about 0.8% w/w, about 0.9% w/w, or about 1% w/w.
• the surfactant is present in the solid dispersion in an amount of about 0.5% w/w, about 1% w/w, about 1.5% w/w, about 2% w/w, about 2.5% w/w, about 3% w/w, about 3.5% w/w, about 4% w/w, about 4.5% w/w, or about 5% w/w.
• the solid dispersion may be prepared according to a process described herein.
• methods that could be used include those that involve rapid removal of solvent or solvent mixture from a mixture or cooling a molten sample. Such methods include, but are not limited to, rotational evaporation, freeze-drying (i.e., lyophilization), vacuum drying, melt congealing, and melt extrusion.
• One embodiment of this disclosure involves solid dispersion obtained by spray-drying. In one embodiment, the product obtained by spray drying is dried to remove the solvent or solvent mixture.
• Preparations disclosed herein can be obtained by spray-drying a mixture comprising COMPOUND 2, one or more polymer(s), and an appropriate solvent or solvent mixture.
• Spray drying involves atomization of a liquid mixture containing, e.g. , a solid and a solvent or solvent mixture, and removal of the solvent or solvent mixture.
• the solvent or solvent mixture can also contain a nonvolatile solvent, such as glacial acetic acid. Atomization may be done, for example, through a two-fluid or pressure or electrosonic nozzle or on a rotating disk.
• Spray drying converts a liquid feed to a dried particulate form.
• Spray drying generally involves the atomization of a liquid feed solution into a spray of droplets and contacting the droplets with hot air or gas in a drying chamber.
• the sprays are generally produced by either rotary (wheel) or nozzle atomizers. Evaporation of moisture from the droplets and formation of dry particles proceed under controlled temperature and airflow conditions.
• a secondary drying process such as fluidized bed drying or vacuum drying, may be used to reduce residual solvents (and other additives, such as glacial acetic acid) to pharmaceutically acceptable levels.
• spray-drying involves contacting a highly dispersed liquid suspension or solution (e.g. , atomized solution), and a sufficient volume of hot air or gas (e.g. , nitrogen, e.g. , pure nitrogen) to produce evaporation and drying of the liquid droplets.
• the preparation to be spray dried can be any solution, coarse suspension, slurry, colloidal dispersion, or paste that may be atomized using the selected spray-drying apparatus.
• the preparation is sprayed into a current of warm filtered air (or into gas, e.g. , nitrogen) that evaporates the solvent and conveys the dried product to a collector (e.g. , a cyclone).
• a collector e.g. , a cyclone
• the spent air or gas is then exhausted with the solvent (or solvent mixture including any additives such as glacial acetic acid), (e.g. , then filtered) or alternatively the spent air or gas is sent to a condenser to capture and potentially recycle the solvent or solvent mixture.
• a gas e.g. , nitrogen
• the gas is then optionally recycled, heated again and returned to the unit in a closed loop system.
• Commercially available types of apparatus may be used to conduct the spray-drying.
• commercial spray dryers are manufactured by Buchi Ltd. and Niro (e.g. , the PSD line of spray driers manufactured by Niro).
• Spray-drying typically employs solids loads of material from about 1% to about 30% or up to about 50% (i.e., therapeutically active compound plus and excipients), preferably at least about 10%. In some embodiments, solids loads of less than 10% may result in poor yields and unacceptably long run-times. In general, the upper limit of solids loads is governed by the viscosity of (e.g. , the ability to pump) the resulting solution and the solubility of the components in the solution. Generally, the viscosity of the solution can determine the size of the particle in the resulting powder product.
• the spray-drying is conducted with an inlet temperature of from about 40°C to about 200°C, for example, from about 70°C to about 150°C, preferably from about 40°C to about 60°C, about 50°C to about 55°C, or about 80°C to about 110°C, e.g. , about 90°C.
• the spray-drying is generally conducted with an outlet temperature of from about 20 °C to about 100°C, for example from about 25°C to about 30°C ⁇ e.g. , about 26°C), about 40°C to about 50°C, about 50°C to about 65°C, e.g. , about 56°C to about 58°C.
• Removal of the solvent or solvent mixture may require a subsequent drying step, such as tray drying, fluid bed drying ⁇ e.g. , from about room temperature to about 100°C), vacuum drying, microwave drying, rotary drum drying or biconical vacuum drying ⁇ e.g. , from about room temperature to about 200°C).
• a subsequent drying step such as tray drying, fluid bed drying ⁇ e.g. , from about room temperature to about 100°C), vacuum drying, microwave drying, rotary drum drying or biconical vacuum drying ⁇ e.g. , from about room temperature to about 200°C).
• the spray-drying is fluidized spray drying (FSD).
• the steps in FSD can include, for example: preparing a liquid feed solution ⁇ e.g. , containing COMPOUND 2, and optionally a polymer(s) and/or surfactant(s), dissolved or suspended in solvent(s)); atomizing ⁇ e.g. , with a pressure nozzle, a rotary atomizer or disk, two-fluid nozzle or other atomizing methods) the feed solution upon delivery into the drying chamber of a spray dryer, e.g. , operating in FSD mode; drying the feed solution in the drying chamber with heated air or a heated gas ⁇ e.g.
• the fines into the drying chamber, wherein the re-introduced fines can agglomerate with newly formed product to generate an agglomerated product, wherein if the agglomerated product is large enough, it will separate out, if it is not large enough to separate out, the agglomerated product will be carried by convection to the top of the chamber and to the cyclone and re-introduced into the chamber. This process repeats until an agglomerated product that is large enough to drop out is formed.
• the fines can be re-introduced from the cyclone to the drying chamber via a feed pipe.
• the feed solution can instead be spray congealed, e.g. , the chamber is at room temperature (e.g. , 21+ 4 °C) or is cooled, e.g. , cooled gas (e.g. , nitrogen) is used for the process.
• room temperature e.g. , 21+ 4 °C
• cooled gas e.g. , nitrogen
• FSD can further include collecting the agglomerated product in a first fluidizing chamber; which can be followed by discharging the agglomerated product from the first fluidizing chamber to a second fluidizing chamber, wherein a post-drying process can occur.
• the agglomerated product (e.g. , that separates out in the drying chamber) can then be transferred from the second fluidizing chamber to a third fluidizing chamber, where the agglomerated product is cooled.
• the agglomerated product (e.g. , a solid dispersion of an amorphous compound) can then be further processed.
• the product can be directly compressed.
• the product can optionally be blended with a surfactant, excipient, or
• the product can optionally be further processed, e.g. , milled, granulated, blended, and/or mixed with a melt granulate, surfactant, excipient, and/or pharmaceutically acceptable carrier.
• FSD can be performed in a commercial spray dryer operating in fluidized spray dryer mode (FSD mode). FSD can be accomplished in either open cycle mode or closed cycle mode (e.g. , the drying gas, e.g. , nitrogen, is recycled).
• suitable spray dryers for use in FSD include dryers from Niro (e.g. , the PSD line of spray driers manufactured by Niro:
• FSD can essentially be performed in any spray dryer that is configured to allow for the re-introduction of fines into the drying chamber.
• Additional post drying e.g. , in a vacuum or fluidized bed dryer or a double cone or biconical post-dryer or a tumble dryer, can be performed if needed/applicable to remove further solvents.
• a post-drying step is performed.
• the dispersion can be directly compressed into a dosage form.
• the inlet temperature is between about 50°C and about 200°C, e.g. , between about 60°C and about 150°C, between about 70°C and about 100°C, between about 60°C and about 95°C, between about 65°C and about 85°C, between about 70°C and about 90°C, between about 85°C and about 95°C, or between about 70°C and about 85°C.
• the outlet temperature is between about room temperature (e.g. , USP room temperature (e.g. , 21+4°C)) and about 80°C, e.g. , between about 25°C and about 75°C, between about 30°C and about 65°C, between about 35°C and about 70°C, between about 40°C and about 65 °C, between about 45 °C and about 60°C, between about 35°C and about 45°C, between about 35°C and about 40°C, or between about 37°C and about 40°C.
• room temperature e.g. , USP room temperature (e.g. , 21+4°C)
• 80°C e.g. , between about 25°C and about 75°C, between about 30°C and about 65°C, between about 35°C and about 70°C, between about 40°C and about 65 °C, between about 45 °C and about 60°C, between about 35°C and about 45°C, between about 35°C and about 40°
• the temperature set points of the fluidized beds is between about room temperature (e.g. , USP room temperature (e.g. , 21+4°C)) and about 100°C, e.g. , between about 30°C and about 95°C, between about 40°C and about 90°C, between about 50°C and about 80°C, between about 60°C and about 85°C, between about 65°C and about 95°C, or between about 80°C and about 95°C.
• FSD can be performed on a mixture containing COMPOUND 2.
• FSD can be performed on a mixture containing COMPOUND 2, and one or more polymer(s), and optionally one or more surfactant(s), and optionally one or more additional excipients(s)) to obtain a solid dispersion of amorphous COMPOUND 2 thereof, e.g. , that can be directly compressed into an oral dosage form (e.g. , tablet).
• the dispersion can be blended with one or more excipients prior to compression.
• the process for preparing a solid dispersion of COMPOUND 2 comprises:
• the solvent is removed by spray drying.
• the solid dispersion is tray dried using a convection tray dryer.
• the solid dispersion is screened.
• COMPOUND 2 is crystalline. In another embodiment,
• COMPOUND 2 is amorphous.
• spray drying may be done and is often done in the presence of an inert gas such as nitrogen.
• processes that involve spray drying may be done in the presence of a supercritical fluid involving carbon dioxide or a mixture including carbon dioxide.
• the process for preparing a solid dispersion of COMPOUND 2 comprises:
• Post-drying and/or polishing the wet spray dried dispersion to below ICH or given specifications for residual solvents can optionally be performed.
• the solvent comprises one or more volatile solvent(s) to dissolve or suspend COMPOUND 2 and the polymer(s). In some embodiments, the one or more solvent(s) completely dissolves COMPOUND 2 and the polymer(s).
• the one or more solvent(s) is a volatile solvent (e.g. , methylene chloride, acetone, methanol, ethanol, chloroform, tetrahydrofuran (THF), or a mixture thereof).
• suitable volatile solvents include those that dissolve or suspend the therapeutically active compound either alone or in combination with another co-solvent.
• the solvent(s) completely dissolves the therapeutically active compound.
• the solvent is acetone.
• the solvent is methanol.
• the solvent is a non- volatile solvent (e.g. , organic acids such as glacial acetic acid, dimethyl sulfoxide (DMSO), dimethylformamide (DMF), or water).
• a non-volatile solvent is a component in a solvent system.
• the non-volatile solvent is present as a component in a solvent from about 1% to about 20% w/w (e.g. , from about 3% w/w to about 15% w/w, from about 4% w/w to about 12% w/w, or from about 5% w/w to about 10% w/w).
• the solvent is a mixture of solvents.
• the solvent can include from about 0% to about 30% acetone and from about 70% to about 100% methanol, or the solvent can include from about 0% to about 40% acetone and from about 60% to about 100% methanol.
• Other exemplary ratios of methanol to acetone include 80:20, 75:25, 70:30, 60:40, 55:45, and 50:50.
• the solvent is a combination of solvents including at least one non-volatile solvent.
• the solvent is a combination of components that includes both a volatile solvent and a non- volatile solvent.
• the solvent system is a combination of a volatile solvent or combination of solvents such as methanol and acetone with a non-volatile solvent such as glacial acetic acid.
• the solvent system comprises from about 40% to about 80% methanol, from about 20% to about 35% acetone, and from about 1% to about 15% glacial acetic acid (e.g. , from about 50% to about 70% methanol, from about 25% to about 30% acetone, and from about 3% to about 12% glacial acetic acid).
• the solvent system is a combination of a volatile solvent or combination of solvents such as methanol and acetone with a non- volatile solvent such as water.
• the solvent system comprises from about 40% to about 80% methanol, from about 20% to about 35% acetone, and from about 0.1% to about 15% water (e.g. , from about 50% to about 70% methanol, from about 25% to about 30% acetone, and from about 1% to about 5% water).
• the pharmaceutical compositions of the solid dispersion may be made by a process described herein.
• compositions comprising: (a) a solid dispersion, comprising COMPOUND 2 and a polymer; and (b) one or more pharmaceutically acceptable carrier(s).
• pharmaceutically acceptable carriers are fillers, disintegrants, wetting agents, glidants, and lubricants.
• the pharmaceutical compositions may be orally administered in any orally acceptable dosage form including, but not limited to, capsules, tablets, emulsions and aqueous suspensions, dispersions and solutions.
• the pharmaceutical composition is a tablet.
• the pharmaceutical composition comprises a directly compressed dosage form of COMPOUND 2.
• the pharmaceutical composition also includes a filler.
• the filler can be, for example, microcrystalline cellulose, lactose, mannitol, ethyl cellulose, sorbitol, starch, sucrose, calcium phosphate, powdered cellulose, silicified microcrystalline cellulose, isomalt, or mixtures thereof.
• the filler is microcrystalline cellulose.
• the filler is present in the pharmaceutical composition in an amount of between about 10% w/w and 50% w/w (e.g. , between about 15% w/w and about 45% w/w; between about 20% w/w and about 40% w/w; between about 25% w/w and about 35% w/w; or between about 28% w/w and about 32% w/w).
• the filler is present in the pharmaceutical composition in an amount of from about 20% w/w to about 35% w/w, for example from about 25% w/w to about 34% w/w, or from about 26% w/w to about 33% w/w, or from about 27% w/w to about 32% w/w, for example, about 28% w/w, about 28.5% w/w, about 29% w/w, about 29.5% w/w about 30% w/w, about 30.5% w/w, about 31% w/w, or about 31.5% w/w.
• the filler is present in the pharmaceutical composition in an amount of about 29% w/w, about 29.1% w/w, about 29.2% w/w, about 29.3% w/w, about 29.4% w/w, about 29.5% w/w, about 29.6% w/w, about 29.7% w/w, about 29.8% w/w, about 29.9% w/w, or about 30% w/w.
• the filler is present in the pharmaceutical composition in an amount of between about 25% w/w and about 35% w/w. In some
• the filler is present in the pharmaceutical composition in an amount of about 29.5% w/w.
• the pharmaceutical composition also includes a disintegrant.
• the disintegrant can be, for example, colloidal silicon dioxide, powdered cellulose, calcium silicate, crospovidone, calcium alginate, methyl cellulose, chitosan, carboxy methyl cellulose, croscarmellose sodium, carboxymethyl starch, sodium alginate, sodium starch glycolate, pregelatinized starch, or mixtures thereof.
• the disintegrant is
• the disintegrant is present in the pharmaceutical composition in an amount of between about 1% w/w and 15% w/w (e.g. , between about 3% w/w and about 12% w/w; between about 4% w/w and about 10% w/w; between about 5% w/w and about 7% w/w; or between about 6% w/w and about 7% w/w).
• the disintegrant is present in the pharmaceutical composition in an amount of about 3% w/w, about 3.5% w/w, about 4% w/w, about 49.5% w/w about 5% w/w, about 5.5% w/w, about 6% w/w, or about 6.5% w/w, about 7% w/w, about 7.5% w/w, about 8% w/w, about 8.5% w/w, about 9% w/w, about 9.5% w/w, or about 10% w/w.
• the disintegrant is present in the pharmaceutical composition in an amount of between about 5% w/w and about 7% w/w. In some embodiments, the disintegrant is present in the pharmaceutical composition in an amount of about 6% w/w.
• the pharmaceutical composition also includes a wetting agent.
• the wetting agent can be, for example, sodium lauryl sulfate, sodium dodecyl sulfate, polysorbates (such as Tween 20 and Tween 80), poloxamers (such as Poloxamer 335 and Poloxamer 407), glyceryl monooleate, or mixtures thereof.
• the wetting agent is sodium lauryl sulfate.
• the wetting agent is present in the pharmaceutical composition in an amount of between about 0.1% w/w and 2% w/w (e.g. , between about 0.5% w/w and about 2% w/w; between about 0.5% w/w and about 1.5% w/w; or between about 1% w/w and about 1.5% w/w).
• the wetting agent is present in the pharmaceutical composition in an amount of about 0.1% w/w, about 0.2% w/w, about 0.3% w/w, about 0.4% w/w about 0.5% w/w, about 0.6% w/w, about 0.7% w/w, or about 0.8% w/w, about 0.9% w/w, about 1% w/w, about 1.1 % w/w, about 1.2% w/w, about 1.3% w/w, about 1.4% w/w, about 1.5% w/w, about 1.6% w/w, about 1.7% w/w, about 1.8% w/w, about 1.9% w/w, or about 2% w/w.
• the wetting agent is present in the pharmaceutical composition in an amount of between about 0.5% w/w and about 1.5% w/w. In some embodiments, the wetting agent is present in the pharmaceutical composition in an amount of about 1% w/w.
• the pharmaceutical composition also includes a glidant.
• the glidant can be, for example, silicon dioxide, colloidal silicon dioxide, tribasic calcium phosphate, magnesium stearate, magnesium trisilicate, powdered cellulose, talc, starch, and mixtures thereof.
• the glidant is colloidal silicon dioxide.
• the glidant is present in the pharmaceutical composition in an amount of between about 0.1% w/w and 5% w/w (e.g. , between about 1% w/w and about 4% w/w; between about 1% w/w and about 3% w/w; or between about 1.5% w/w and about 2.5% w/w).
• the glidant is present in the pharmaceutical composition in an amount of about 0.5% w/w, about 1% w/w, about 1.5% w/w, about 2% w/w about 2.5% w/w, about 3% w/w, about 3.5% w/w, or about 4% w/w, about 4.5% w/w, or about 5% w/w.
• the glidant is present in the pharmaceutical composition in an amount of about 1.1% w/w, about 1.2% w/w, about 1.3% w/w, about 1.4% w/w, about 1.5% w/w, about 1.6% w/w, about 1.7% w/w, about 1.8% w/w, about 1.9% w/w, about 2% w/w, 2.1% w/w, about 2.2% w/w, about 2.3% w/w, about 2.4% w/w, about 2.5% w/w, about 2.6% w/w, about 2.7% w/w, about 2.8% w/w, about 2.9% w/w, or about 3% w/w.
• the glidant is present in the pharmaceutical composition in an amount of between about 1% w/w and about 3% w/w. In some embodiments, the glidant is present in the pharmaceutical composition in an amount of about 2% w/w.
• the pharmaceutical composition also includes a lubricant.
• the lubricant can be, for example, magnesium stearate, talc, sodium stearyl fumarate, glyceryl behenate, hydrogenated vegetable oil, zinc stearate, calcium stearate, sucrose stearate, polyvinyl alcohol, magnesium lauryl sulfate, or mixtures thereof.
• the lubricant is magnesium stearate.
• the lubricant is present in the pharmaceutical composition in an amount of between about 0.1% w/w and 5% w/w (e.g. , between about 1% w/w and about 4% w/w; between about 1% w/w and about 3% w/w; or between about 1% w/w and about 2% w/w).
• the lubricant is present in the pharmaceutical composition in an amount of about 0.5% w/w, about 1% w/w, about 1.5% w/w, about 2% w/w about 2.5% w/w, about 3% w/w, about 3.5% w/w, or about 4% w/w, about 4.5% w/w, or about 5% w/w.
• the lubricant is present in the pharmaceutical composition in an amount of about 0.1% w/w, about 0.2% w/w, about 0.3% w/w, about 0.4% w/w, about 0.5% w/w, about 0.6% w/w, about 0.7% w/w, about 0.8% w/w, about 0.9% w/w, about 1% w/w, about 1.1% w/w, about 1.2% w/w, about 1.3% w/w, about 1.4% w/w, about 1.5% w/w, about 1.6% w/w, about 1.7% w/w, about 1.8% w/w, about 1.9% w/w, about 2% w/w, 2.1% w/w, about 2.2% w/w, about 2.3% w/w, about 2.4% w/w, or about 2.5% w/w.
• the lubricant is present in the pharmaceutical composition in an amount of between about 0.5% w/w and about 2.5% w/w. In some embodiments, the lubricant is present in the pharmaceutical composition in an amount of about 1.5% w/w.
• the solid dispersion makes up about 25% to 85% by weight of the total weight of the pharmaceutical composition. In some embodiments, the solid dispersion makes up about 50% to about 70% by weight of the total weight of the pharmaceutical composition.
• the COMPOUND 2 makes up about 15% to 45% of the total weight of the pharmaceutical composition, and the one or more polymer(s) makes up about 15% to 45% of the total weight of the pharmaceutical composition.
• the COMPOUND 2 makes up about 20% w/w of the pharmaceutical composition
• the one or more polymer(s) makes up about 40% w/w of the pharmaceutical composition.
• the COMPOUND 2 makes up about 25% w/w of the pharmaceutical composition
• the one or more polymer(s) makes up about 35% w/w of the pharmaceutical composition.
• the COMPOUND 2 makes up about 30% w/w of the pharmaceutical composition
• the one or more polymer(s) makes up about 30% w/w of the pharmaceutical composition.
• the COMPOUND 2 makes up about 35% w/w of the pharmaceutical composition
• the one or more polymer(s) makes up about 25% w/w of the pharmaceutical composition.
• the solid dispersion makes up from between about 50% w/w to about 70% w/w of the pharmaceutical composition
• the filler makes up from between about 25% w/w to about 35% w/w of the pharmaceutical composition
• the disintegrant makes up from between about 5% w/w to about 7% w/w of the pharmaceutical composition
• the wetting agent makes up from between about 0.5% w/w to about 1.5% w/w of the pharmaceutical composition
• the glidant makes up from between about 1% w/w to about 3% w/w of the pharmaceutical composition
• the lubricant makes up from between about 0.5% w/w to about 2.5% w/w of the pharmaceutical composition thereby totaling 100% by weight of the composition.
• the solid dispersion makes up about 60% w/w of the pharmaceutical composition
• the filler makes up about 29.5% w/w of the pharmaceutical composition
• the disintegrant makes up about 6% w/w of the pharmaceutical composition
• the wetting agent makes up about 1% w/w of the pharmaceutical composition
• the glidant makes up about 2% w/w of the pharmaceutical composition
• the lubricant makes up about 1.5% w/w of the pharmaceutical composition.
• the pharmaceutical composition comprises, from between about 25% w/w to about 35% w/w of COMPOUND 2 from between about 25% w/w to about 35% w/w of hypromellose acetate succinate (HPMCAS), from between about 25% w/w to about 35% w/w of microcrystalline cellulose, from between about 5% w/w to about 7% w/w
• HPMCAS hypromellose acetate succinate
• croscarmellose sodium from between about 0.5% w/w to about 1.5% w/w sodium lauryl sulfate, about from between about 1% w/w to about 3% w/w colloidal silicon dioxide, and rom between about 0.5% w/w to about 2.5% w/w of magnesium stearate, thereby totaling 100% by weight of the composition.
• the pharmaceutical composition comprises, about 30% w/w of COMPOUND 2 about 30% w/w of hypromellose acetate succinate (HPMCAS), about 29.5% w/w of microcrystalline cellulose, about 6% w/w croscarmellose sodium, about 1% w/w sodium lauryl sulfate, about 2% w/w colloidal silicon dioxide, and about 1.5% w/w of magnesium stearate.
• HPMCAS hypromellose acetate succinate
• the solid dispersion, filler, disintegrant, wetting agent, glidant, and lubricant are added intragranularly. In some embodiments, an additional amount of the filler, disintegrant, glidant, and lubricant are added extragranularly.
• the pharmaceutical composition comprises, the following intragranularly added components: the solid dispersion makes up from about 50% w/w to about 70% w/w of the pharmaceutical composition, the filler makes up from about 18% w/w to about 26% w/w of the pharmaceutical composition, disintegrant makes up from about 2% w/w to about 6% w/w of the pharmaceutical composition, wetting agent makes up from about 0.5% w/w to about 1.5% w/w of the pharmaceutical composition, glidant makes up from about 0.5% w/w to about 1.5% w/w of the pharmaceutical composition, and lubricant makes up from about 0.25% w/w to about 1% w/w of the pharmaceutical composition.
• the solid dispersion makes up from about 50% w/w to about 70% w/w of the pharmaceutical composition
• the filler makes up from about 18% w/w to about 26% w/w of the pharmaceutical composition
• disintegrant makes up from about 2% w/w to about 6% w/w of the pharmaceutical
• a the pharmaceutical composition comprises the following extragranularly added components: an additional amount of the filler makes up from about 4% w/w to about 12% w/w of the pharmaceutical composition, an additional amount of the disintegrant makes up from about 1% w/w to about 3% w/w of the pharmaceutical composition, an additional amount of the glidant makes up from about 0.5% w/w to about 1.5% w/w of the pharmaceutical composition, and an additional amount of the lubricant makes up from about 0.5% w/w to about 1.5% w/w of the pharmaceutical composition, and are added extragranularly.
• the pharmaceutical composition comprises, the following intragranularly added components: the solid dispersion makes up about 60% w/w of the pharmaceutical composition, the filler makes up about 21.5% w/w of the pharmaceutical composition, disintegrant makes up about 4% w/w of the pharmaceutical composition, wetting agent makes up about 1% w/w of the pharmaceutical composition, glidant makes up about 1% w/w of the pharmaceutical composition, and lubricant makes up about 0.5% w/w of the pharmaceutical composition.
• the solid dispersion makes up about 60% w/w of the pharmaceutical composition
• the filler makes up about 21.5% w/w of the pharmaceutical composition
• disintegrant makes up about 4% w/w of the pharmaceutical composition
• wetting agent makes up about 1% w/w of the pharmaceutical composition
• glidant makes up about 1% w/w of the pharmaceutical composition
• lubricant makes up about 0.5% w/w of the pharmaceutical composition.
• a the pharmaceutical composition comprises the following extragranularly added components: an additional amount of the filler makes up about 8% w/w of the pharmaceutical composition, an additional amount of the disintegrant makes up about 2% w/w of the pharmaceutical composition, an additional amount of the glidant makes up about 1% w/w of the pharmaceutical composition, and an additional amount of the lubricant makes up about 1% w/w of the pharmaceutical composition, and are added extragranularly.
• the pharmaceutical composition comprises, the following intragranularly added components: the solid dispersion comprising COMPOUND 2 and hypromellose acetate succinate (HPMCAS), makes up from about 50% w/w to about 70% w/w of the pharmaceutical composition, microcrystalline cellulose makes up from about 18% w/w to about 26% w/w of the pharmaceutical composition, croscarmellose sodium makes up from about 2% w/w to about 6% w/w of the pharmaceutical composition, sodium lauryl sulfate makes up from about 0.5% w/w to about 1.5% w/w of the pharmaceutical composition, colloidal silicon dioxide makes up from about 0.5% w/w to about 1.5% w/w of the pharmaceutical composition, and magnesium stearate makes up from about 0.25% w/w to about 1% w/w of the solid dispersion comprising COMPOUND 2 and hypromellose acetate succinate (HPMCAS), makes up from about 50% w/w to about 70% w/w of the pharmaceutical composition, microcrystalline
• a the pharmaceutical composition comprises the following extragranularly added components: an additional amount of microcrystalline cellulose makes up from about 4% w/w to about 12% w/w of the pharmaceutical composition, an additional amount of croscarmellose sodium makes up from about 1% w/w to about 3% w/w of the pharmaceutical composition, an additional amount of colloidal silicon dioxide makes up from about 0.5% w/w to about 1.5% w/w of the pharmaceutical composition, and an additional amount of magnesium stearate makes up from about 0.5% w/w to about 1.5% w/w of the pharmaceutical composition, and are added extragranularly.
• the pharmaceutical composition comprises, the following intragranularly added components: the solid dispersion comprising COMPOUND 2 and hypromellose acetate succinate (HPMCAS), makes up about 60% w/w of the pharmaceutical composition, microcrystalline cellulose makes up about 21.5% w/w of the pharmaceutical composition, croscarmellose sodium makes up about 4% w/w of the pharmaceutical
• HPMCAS hypromellose acetate succinate
• sodium lauryl sulfate makes up about 1% w/w of the pharmaceutical composition
• colloidal silicon dioxide makes up about 1% w/w of the pharmaceutical composition
• magnesium stearate makes up about 0.5% w/w of the pharmaceutical composition.
• a the pharmaceutical composition comprises the following extragranularly added components: an additional amount of microcrystalline cellulose makes up about 8% w/w of the pharmaceutical composition, an additional amount of croscarmellose sodium makes up about 2% w/w of the pharmaceutical composition, an additional amount of colloidal silicon dioxide makes up about 1% w/w of the pharmaceutical composition, and an additional amount of magnesium stearate makes up about 1% w/w of the pharmaceutical composition, and are added extragranularly.
• the inhibitory activity of COMPOUND 2 against IDH1 mutants can be tested by methods described in Example A of PCT Publication No. WO 2013/107291 and US Publication No. US 2013/0190249, hereby incorporated by reference in their entireties, or analogous methods.
• hematologic malignancy is an advanced hematologic malignancy.
• provided herein is a method of treating solid tumors by administering to a subject a combination of a mutant IDHl inhibitor and a DNA demethylating agent.
• the mutant IDHl inhibitor is COMPOUND 2.
• the DNA demethylating agent is azacitidine.
• provided herein is a method of treating hematologic
• MDS myelodysplastic syndrome
• MPN myeloproliferative neoplasms
• CMML chronic myelomonocytic leukemia
• B-ALL B-acute lymphoblastic leukemias
• lymphoma e.g., T-cell lymphoma
• the hematologic malignancy is an advanced hematologic malignancy.
• provided herein is a method of treating hematologic
• hematologic malignancies such as acute myelogenous leukemia (AML), myelodysplastic syndrome (MDS), myeloproliferative neoplasms (MPN), chronic myelomonocytic leukemia (CMML), B-acute lymphoblastic leukemias (B-ALL), or lymphoma (e.g., T-cell lymphoma), each characterized by the presence of a mutant allele of IDHl, comprising administering to a subject a therapeutically effective amount of COMPOUND 2, or a solid suspension thereof, and azacitidine.
• the hematologic malignancy is an advanced hematologic malignancy.
• hematologic malignancies such as acute myelogenous leukemia (AML), myelodysplastic syndrome (MDS), myeloproliferative neoplasms (MPN), chronic myelomonocytic leukemia (CMML), B-acute lymphoblastic leukemias (B-ALL), or lymphoma (e.g., T-cell lymphoma), each characterized by the presence of a mutant allele of IDHl, comprising administering to a subject a therapeutically effective amount of a single crystalline form of COMPOUND 2 and azacitidine.
• the single crystalline form of COMPOUND 2 is any percentage between 90% and 100% pure.
• the hematologic malignancy is an advanced hematologic malignancy.
• provided herein is a method of treating hematologic
• hematologic malignancies such as acute myelogenous leukemia (AML), myelodysplastic syndrome (MDS), myeloproliferative neoplasms (MPN), chronic myelomonocytic leukemia (CMML), B-acute lymphoblastic leukemias (B-ALL), or lymphoma (e.g., T-cell lymphoma), each characterized by the presence of a mutant allele of IDHl, comprising administering to a subject a pharmaceutical composition comprising a therapeutically effective amount of COMPOUND 2 and azacitidine.
• the hematologic malignancy is an advanced hematologic malignancy.
• a method of treating an hematologic malignancy such as acute myelogenous leukemia (AML), myelodysplastic syndrome (MDS), myeloproliferative neoplasms (MPN), chronic myelomonocytic leukemia (CMML), B-acute lymphoblastic leukemias (B-ALL), or lymphoma (e.g., T-cell lymphoma), each characterized by the presence of a mutant allele of IDHl, comprising administering to a subject a pharmaceutical composition comprising a therapeutically effective amount of a single crystalline form of COMPOUND 2 and azacitidine.
• the single crystalline form of COMPOUND 2 and azacitidine.
• COMPOUND 2 is any percentage between 90% and 100% pure.
• the hematologic malignancy is an advanced hematologic malignancy.
• a method of treating a solid tumor such as glioma, melanoma, chondrosarcoma, cholangiocarcinoma, sarcoma, or non-small cell lung cancer, each characterized by the presence of a mutant allele of IDHl, comprising administering to a subject a therapeutically effective amount of COMPOUND 2, or a crystalline form thereof, and azacitidine.
• a method of treating a solid tumor such as glioma, melanoma, chondrosarcoma, cholangiocarcinoma, sarcoma, or non-small cell lung cancer, each characterized by the presence of a mutant allele of IDHl, comprising administering to a subject a therapeutically effective amount of COMPOUND 2 and azacitidine.
• a method of treating a solid tumor such as glioma, melanoma, chondrosarcoma, cholangiocarcinoma (including intrahepatic cholangiocarcinoma (IHCC), prostate cancer, colon cancer, or non-small cell lung cancer (NSCLC), each characterized by the presence of a mutant allele of IDH1, comprising administering to a subject a therapeutically effective amount of COMPOUND 2, or a solid suspension thereof, and azacitidine.
• a solid tumor such as glioma, melanoma, chondrosarcoma, cholangiocarcinoma (including intrahepatic cholangiocarcinoma (IHCC), prostate cancer, colon cancer, or non-small cell lung cancer (NSCLC), each characterized by the presence of a mutant allele of IDH1, comprising administering to a subject a therapeutically effective amount of COMPOUND 2, or a solid suspension thereof, and azacitidine.
• COMPOUND 2 intra
• a solid tumor such as glioma, melanoma, chondrosarcoma, cholangiocarcinoma (including intrahepatic
• cholangiocarcinoma IHCC
• prostate cancer colon cancer
• NSCLC non-small cell lung cancer
• COMPOUND 2 is any percentage between 90% and 100% pure.
• a method of treating a solid tumor such as glioma, melanoma, chondrosarcoma, cholangiocarcinoma (including intrahepatic
• cholangiocarcinoma IHCC
• prostate cancer colon cancer
• NSCLC non-small cell lung cancer
• a method of treating a solid tumor such as glioma, melanoma, chondrosarcoma, cholangiocarcinoma (including intrahepatic
• cholangiocarcinoma IHCC
• prostate cancer colon cancer
• NSCLC non-small cell lung cancer
• each characterized by the presence of a mutant allele of IDH1 comprising administering to a subject a pharmaceutical composition comprising a therapeutically effective amount of a single crystalline form of COMPOUND 2 and azacitidine.
• the single crystalline form of COMPOUND 2 is any percentage between 90% and 100% pure.
• the malignancy to be treated is characterized by a mutant allele of IDH1, wherein the IDH1 mutation results in a new ability of the enzyme to catalyze the NAPH dependent reduction of a ketoglutarate to R( ) 2 hydroxyglutarate in a patient.
• the mutant ⁇ 3 ⁇ 1 has an R132X mutation.
• the R132X mutation is selected from R132H, R132C, R132L, R132V, R132S and R132G.
• the R132X mutation is R132H or R132C.
• the R132X mutation is R132H.
• mutant alleles of IDHl wherein the IDHl mutation results in a new ability of the enzyme to catalyze the NAPH- dependent reduction of a-ketoglutarate to R(-)-2-hydroxyglutarate, and in particular R132H mutations of IDHl, characterize a subset of all types of cancers, without regard to their cellular nature or location in the body.
• the compounds, and methods desribed herein are useful to treat an hematologic malignancy, including an advanced hematologic malignancy, such as acute myelogenous leukemia (AML), myelodysplastic syndrome (MDS), myeloproliferative neoplasms (MPN), chronic myelomonocytic leukemia (CMML), B-acute lymphoblastic leukemias (B-ALL), or lymphoma (e.g., T-cell lymphoma), each characterized by the presence of a mutant allele of IDHl imparting such activity and in particular an IDHl R132H or R132C mutation.
• AML acute myelogenous leukemia
• MDS myelodysplastic syndrome
• MPN myeloproliferative neoplasms
• CMML chronic myelomonocytic leukemia
• B-ALL B-acute lymphoblastic leukemias
• lymphoma e.g.
• the compounds, and methods desribed herein are useful to treat a solid tumor, such as glioma, melanoma, chondrosarcoma, cholangiocarcinoma (including intrahepatic cholangiocarcinoma (IHCC), prostate cancer, colon cancer, or non-small cell lung cancer (NSCLC), each characterized by the presence of a mutant allele of IDHl imparting such activity and in particular an IDHl R132H or R132C mutation.
• a solid tumor such as glioma, melanoma, chondrosarcoma, cholangiocarcinoma (including intrahepatic cholangiocarcinoma (IHCC), prostate cancer, colon cancer, or non-small cell lung cancer (NSCLC), each characterized by the presence of a mutant allele of IDHl imparting such activity and in particular an IDHl R132H or R132C mutation.
• the malignancy is a tumor wherein at least 30, 40, 50, 60, 70, 80 or 90% of the tumor cells carry an IDHl mutation, and in particular an an IDHl R132H or R132C mutation, at the time of diagnosis or treatment.
• the efficacy of treatment of malignancy is monitored by measuring the levels of 2HG in the subject. Typically levels of 2HG are measured prior to treatment, wherein an elevated level is indicated for the use of COMPOUND 2. Once the elevated levels are established, the level of 2HG is determined during the course of and/or following termination of treatment to establish efficacy. In certain embodiments, the level of 2HG is only determined during the course of and/or following termination of treatment. A reduction of 2HG levels during the course of treatment and following treatment is indicative of efficacy. Similarly, a determination that 2HG levels are not elevated during the course of or following treatment is also indicative of efficacy.
• 2HG measurements are utilized together with other well-known determinations of efficacy of malignancy treatment, such as reduction in number and size of tumors and/or other cancer-associated lesions, improvement in the general health of the subject, and alterations in other biomarkers that are associated with malignancy treatment efficacy.
• 2HG can be detected in a sample by LC/MS.
• the sample is mixed 80:20 with methanol, and centrifuged at 3,000 rpm for 20 minutes at 4 degrees Celsius.
• the resulting supernatant can be collected and stored at -80 degrees Celsius prior to LC-MS/MS to assess 2-hydroxyglutarate levels.
• LC liquid chromatography
• Each method can be coupled by negative electrospray ionization (ESI, -3.0 kV) to triple-quadrupole mass spectrometers operating in multiple reaction monitoring (MRM) mode, with MS parameters optimized on infused metabolite standard solutions.
• ESI, -3.0 kV negative electrospray ionization
• MRM multiple reaction monitoring
• Metabolites can be separated by reversed phase chromatography using 10 mM tributyl- amine as an ion pairing agent in the aqueous mobile phase, according to a variant of a previously reported method (Luo et al. J Chromatogr A 1147, 153-64, 2007).
• Another method is specific for 2-hydroxyglutarate, running a fast linear gradient from 50% -95% B (buffers as defined above) over 5 minutes.
• a Synergi Hydro-RP, 100mm x 2 mm, 2.1 ⁇ particle size (Phenomonex) can be used as the column, as described above.
• Metabolites can be quantified by comparison of peak areas with pure metabolite standards at known concentration. Metabolite flux studies from
• 13 C-glutamine can be performed as described, e.g., in Munger et al. Nat Biotechnol 26, 1179-86, 2008.
• 2HG is directly evaluated.
• a derivative of 2HG formed in process of performing the analytic method is evaluated.
• a derivative can be a derivative formed in MS analysis.
• Derivatives can include a salt adduct, e.g., a Na adduct, a hydration variant, or a hydration variant which is also a salt adduct, e.g., a Na adduct, e.g., as formed in MS analysis.
• a metabolic derivative of 2HG is evaluated.
• examples include species that build up or are elevated, or reduced, as a result of the presence of 2HG, such as glutarate or glutamate that will be correlated to 2HG, e.g., R-2HG.
• Exemplary 2HG derivatives include dehydrated derivatives such as the compounds provided below or a salt adduct thereof:
• 2HG is known to accumulate in the inherited metabolic disorder 2-hydroxyglutaric aciduria. This disease is caused by deficiency in the enzyme 2-hydroxyglutarate dehydrogenase, which converts 2HG to a-KG (Struys, E. A. et al. Am J Hum Genet 76, 358-60 (2005)).
• 2-hydroxyglutarate dehydrogenase deficiencies accumulate 2HG in the brain as assessed by MRI and CSF analysis, develop leukoencephalopathy, and have an increased risk of developing brain tumors (Aghili, M., Zahedi, F. & Rafiee, J Neurooncol 91, 233-6 (2009); Kolker, S., Mayatepek, E. & Hoffmann, G.
• transaminases which allow utilization of glutamate nitrogen for amino and nucleic acid biosynthesis, and aKG-dependent prolyl hydroxylases such as those which regulate Hif 1-alpha levels.
• provided herein is a method of treating 2-hydroxyglutaric aciduria, particularly D-2-hydroxyglutaric aciduria, in a subject by
• Treatment methods described herein can additionally comprise various evaluation steps prior to and/or following treatment with COMPOUND 2 and azacitidine.
• the method prior to and/or after treatment with COMPOUND 2 and azacitidine, the method further comprises the step of evaluating the growth, size, weight, invasiveness, stage and/or other phenotype of the malignancy.
• the method prior to and/or after treatment with COMPOUND and azacitidine, the method further comprises the step of evaluating the IDH1 genotype of the malignancy. This may be achieved by ordinary methods in the art, such as DNA sequencing, immuno analysis, and/or evaluation of the presence, distribution or level of 2HG.
• the method prior to and/or after treatment with COMPOUND 2 and azacitidine, the method further comprises the step of determining the 2HG level in the subject.
• This may be achieved by spectroscopic analysis, e.g., magnetic resonance-based analysis, e.g., MRI and/or MRS measurement, sample analysis of bodily fluid, such as serum or spinal cord fluid analysis, or by analysis of surgical material, e.g., by mass-spectroscopy.
• COMPOUND 2 and azacitidine are administered concurrently. In one embodiment, COMPOUND 2 and azacitidine are administered sequentially.
• COMPOUND 2 may be administered by oral, parenteral (e.g. , intramuscular, intraperitoneal, intravenous, CTV, intracistemal injection or infusion, subcutaneous injection, or implant), inhalation, nasal, vaginal, rectal, sublingual, or topical (e.g. , transdermal or local) routes of administration.
• COMPOUND 2 may be formulated alone or together with one or more active agent(s), in suitable dosage unit with pharmaceutically acceptable excipients, carriers, adjuvants and vehicles, appropriate for each route of administration.
• the amount of COMPOUND 2 administered in the methods provided herein may range, e.g. , between about 5 mg/day and about 2,000 mg/day. In one embodiment, the range is between about 10 mg/day and about 2,000 mg/day. In one
• the range is between about 20 mg/day and about 2,000 mg/day. In one
• the range is between about 50 mg/day and about 1,000 mg/day. In one
• the range is between about 100 mg/day and about 1,000 mg/day. In one
• the range is between about 100 mg/day and about 500 mg/day. In one embodiment, the range is between about 150 mg/day and about 500 mg/day. In one embodiment, the range is or between about 150 mg/day and about 250 mg/day. In certain embodiments, particular dosages are, e.g. , about 10 mg/day. In one embodiment, the dose is about 20 mg/day. In one
• the dose is about 50 mg/day. In one embodiment, the dose is about 75 mg/day. In one embodiment, the dose is about 100 mg/day. In one embodiment, the dose is about 120 mg/day. In one embodiment, the dose is about 150 mg/day. In one embodiment, the dose is about 200 mg/day. In one embodiment, the dose is about 250 mg/day. In one embodiment, the dose is about 300 mg/day. In one embodiment, the dose is about 350 mg/day. In one embodiment, the dose is about 400 mg/day. In one embodiment, the dose is about 450 mg/day. In one embodiment, the dose is about 500 mg/day. In one embodiment, the dose is about 600 mg/day. In one embodiment, the dose is about 700 mg/day.
• the dose is about 800 mg/day. In one embodiment, the dose is about 900 mg/day. In one embodiment, the dose is about 1,000 mg/day. In one embodiment, the dose is about 1,200 mg/day. In one embodiment, the dose is or about 1,500 mg/day. In certain embodiments, particular dosages are, e.g. , up to about 10 mg/day. In one embodiment, the particular dose is up to about 20 mg/day. In one embodiment, the particular dose is up to about 50 mg/day. In one embodiment, the particular dose is up to about 75 mg/day. In one embodiment, the particular dose is up to about 100 mg/day. In one embodiment, the particular dose is up to about 120 mg/day. In one embodiment, the particular dose is up to about 150 mg/day.
• particular dosages are, e.g. , up to about 10 mg/day. In one embodiment, the particular dose is up to about 20 mg/day. In one embodiment, the particular dose is up to about 50 mg/day. In one embodiment, the particular dose is up to about 75 mg/
• the particular dose is up to about 200 mg/day. In one embodiment, the particular dose is up to about 250 mg/day. In one embodiment, the particular dose is up to about 300 mg/day. In one embodiment, the particular dose is up to about 350 mg/day. In one embodiment, the particular dose is up to about 400 mg/day. In one embodiment, the particular dose is up to about 450 mg/day. In one embodiment, the particular dose is up to about 500 mg/day. In one embodiment, the particular dose is up to about 600 mg/day. In one embodiment, the particular dose is up to about 700 mg/day. In one embodiment, the particular dose is up to about 800 mg/day. In one embodiment, the particular dose is up to about 900 mg/day. In one embodiment, the particular dose is up to about 1,000 mg/day. In one embodiment, the particular dose is up to about 1,200 mg/day. In one embodiment, the particular dose is up to about 1,500 mg/day.
• the amount of COMPOUND 2 in the pharmaceutical is the amount of COMPOUND 2 in the pharmaceutical
• composition or dosage form provided herein may range, e.g. , between about 5 mg and about 2,000 mg. In one embodiment, the range is between about 10 mg and about 2,000 mg. In one embodiment, the range is between about 20 mg and about 2,000 mg. In one embodiment, the range is between about 50 mg and about 1,000 mg. In one embodiment, the range is between about 50 mg and about 500 mg. In one embodiment, the range is between about 50 mg and about 250 mg. In one embodiment, the range is between about 100 mg and about 500 mg. In one embodiment, the range is between about 150 mg and about 500 mg. In one embodiment, the range is between about 150 mg and about 250 mg. In certain embodiments, particular amounts are, e.g. , about 10 mg. In one embodiment, the particular amount is about 20 mg.
• the particular amount is about 50 mg. In one embodiment, the particular amount is about 75 mg. In one embodiment, the particular amount is about 100 mg. In one embodiment, the particular amount is about 120 mg. In one embodiment, the particular amount is about 150 mg. In one embodiment, the particular amount is about 200 mg. In one embodiment, the particular amount is about 250 mg. In one embodiment, the particular amount is about 300 mg. In one embodiment, the particular amount is about 350 mg. In one embodiment, the particular amount is about 400 mg. In one embodiment, the particular amount is about 450 mg. In one embodiment, the particular amount is about 500 mg. In one embodiment, the particular amount is about 600 mg. In one embodiment, the particular amount is about 700 mg. In one embodiment, the particular amount is about 800 mg.
• the particular amount is about 900 mg. In one embodiment, the particular amount is about 1,000 mg. In one embodiment, the particular amount is about 1,200 mg. In one embodiment, the particular amount is or about 1,500 mg. In certain embodiments, particular amounts are, e.g. , up to about 10 mg. In one embodiment, the particular amount is up to about 20 mg. In one embodiment, the particular amount is up to about 50 mg. In one embodiment, the particular amount is up to about 75 mg. In one embodiment, the particular amount is up to about 100 mg. In one embodiment, the particular amount is up to about 120 mg. In one embodiment, the particular amount is up to about 150 mg. In one embodiment, the particular amount is up to about 200 mg. In one embodiment, the particular amount is up to about 250 mg.
• the particular amount is up to about 300 mg. In one embodiment, the particular amount is up to about 350 mg. In one embodiment, the particular amount is up to about 400 mg. In one embodiment, the particular amount is up to about 450 mg. In one embodiment, the particular amount is up to about 500 mg. In one embodiment, the particular amount is up to about 600 mg. In one embodiment, the particular amount is up to about 700 mg. In one embodiment, the particular amount is up to about 800 mg. In one embodiment, the particular amount is up to about 900 mg. In one embodiment, the particular amount is up to about 1,000 mg. In one embodiment, the particular amount is up to about 1,200 mg. In one embodiment, the particular amount is up to about 1,500 mg.
• COMPOUND 2 can be delivered as a single dose such as, e.g. , a single bolus injection, or oral tablets or pills; or over time such as, e.g. , continuous infusion over time or divided bolus doses over time.
• comound 1 can be administered repetitively if necessary, for example, until the patient experiences stable disease or regression, or until the patient experiences disease progression or unacceptable toxicity. Stable disease or lack thereof is determined by methods known in the art such as evaluation of patient' s symptoms, physical examination, visualization of the tumor that has been imaged using X-ray, CAT, PET, or MRI scan and other commonly accepted evaluation modalities.
• COMPOUND 2 is administered to a patient in cycles (e.g. , daily administration for one week, then a rest period with no administration for up to three weeks). Cycling therapy involves the administration of an active agent for a period of time, followed by a rest for a period of time, and repeating this sequential administration. Cycling therapy can reduce the development of resistance, avoid or reduce the side effects, and/or improves the efficacy of the treatment.
• cycles e.g. , daily administration for one week, then a rest period with no administration for up to three weeks. Cycling therapy involves the administration of an active agent for a period of time, followed by a rest for a period of time, and repeating this sequential administration. Cycling therapy can reduce the development of resistance, avoid or reduce the side effects, and/or improves the efficacy of the treatment.
• a method provided herein comprises administering
• the median number of cycles administered in a group of patients is about 1. In one embodiment, the median number of cycles administered in a group of patients is about 2. In one embodiment, the median number of cycles administered in a group of patients is about 3. In one embodiment, the median number of cycles administered in a group of patients is about 4. In one embodiment, the median number of cycles administered in a group of patients is about 5. In one embodiment, the median number of cycles administered in a group of patients is about 6.
• the median number of cycles administered in a group of patients is about 7. In one embodiment, the median number of cycles administered in a group of patients is about 8. In one embodiment, the median number of cycles administered in a group of patients is about 9. In one embodiment, the median number of cycles administered in a group of patients is about 10. In one embodiment, the median number of cycles administered in a group of patients is about 11. In one embodiment, the median number of cycles administered in a group of patients is about 12. In one embodiment, the median number of cycles administered in a group of patients is about 13. In one embodiment, the median number of cycles administered in a group of patients is about 14. In one embodiment, the median number of cycles administered in a group of patients is about 15. In one embodiment, the median number of cycles administered in a group of patients is about 16.
• the median number of cycles administered in a group of patients is about 17. In one embodiment, the median number of cycles administered in a group of patients is about 18. In one embodiment, the median number of cycles administered in a group of patients is about 19. In one embodiment, the median number of cycles administered in a group of patients is about 20. In one embodiment, the median number of cycles administered in a group of patients is about 21. In one embodiment, the median number of cycles administered in a group of patients is about 22. In one embodiment, the median number of cycles administered in a group of patients is about 23. In one embodiment, the median number of cycles administered in a group of patients is about 24. In one embodiment, the median number of cycles administered in a group of patients is about 25.
• the median number of cycles administered in a group of patients is about 26. In one embodiment, the median number of cycles administered in a group of patients is about 27. In one embodiment, the median number of cycles administered in a group of patients is about 28. In one embodiment, the median number of cycles administered in a group of patients is about 29. In one embodiment, the median number of cycles administered in a group of patients is about 30. In one embodiment, the median number of cycles administered in a group of patients is greater than about 30 cycles.
• treatment cycles comprise multiple doses of COMPOUND 2 administered to a subject in need thereof over multiple days (e.g. , 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, or greater than 14 days), optionally followed by treatment dosing holidays (e.g. , 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, or greater than 28 days).
• days e.g. 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, or greater than 14 days
• treatment dosing holidays e.g. , 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, or greater than 28 days.
• azacitidine may be administered by oral, parenteral (e.g. , intramuscular,
• Azacitidine may be formulated, alone or together with COMPOUND 2 and/or one or more active agent(s), in suitable dosage unit with pharmaceutically acceptable excipients, carriers, adjuvants and vehicles, appropriate for each route of administration.
• azacitidine is administered by, e.g. , intravenous (IV), subcutaneous (SC) or oral routes.
• IV intravenous
• SC subcutaneous
• Certain embodiments herein provide co-administration of azacitidine with COMPOUND 2 and/or one or more additional active agents to provide a synergistic therapeutic effect in subjects in need thereof.
• the co-administered active agent(s) may be cancer therapeutic agents, as described herein.
• the co- administered active agent(s) may be inhibitors of IDH1.
• the coadministered agent(s) may be dosed, e.g. , orally or by injection (e.g. , IV or SC).
• treatment cycles comprise multiple doses of azacitidine administered to a subject in need thereof over multiple days (e.g. , 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, or greater than 14 days), optionally followed by treatment dosing holidays (e.g. , 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, or greater than 28 days).
• Suitable dosage amounts for the methods provided herein include, e.g. , therapeutically effective amounts and prophylactically effective amounts.
• the amount of azacitidine is between about 100 mg/m /day and about 1,000
• the amount of azacitidine is between about 100 mg/m /day and about 500 mg/m /day. In certain embodiments, the amount of azacitidine is between about
• the amount of azacitidine is
• azacitidine is between about 50 mg/m /day and about 100 mg/m /day. In certain embodiments,
• the amount of azacitidine is between about 50 mg/m /day and about 75 mg/m /day. In certain embodiments, the amount of azacitidine is between about 120 mg/m /day and about 250
• the particular dosage is about 50 mg/m /day. In one embodiment, the particular dosage is about 60 mg/m /day. In one embodiment, the particular
• 2 2 dosage is about 75 mg/m /day. In one embodiment, the particular dosage is about 80 mg/m /day. In one embodiment, the particular dosage is about 100 mg/m /day. In one embodiment, the particular dosage is about 120 mg/m /day. In one embodiment, the particular dosage is about 140
• the particular dosage is about 150 mg/m /day. In one embodiment, the particular dosage is about 150 mg/m /day. In one
• the particular dosage is about 180 mg/m /day. In one embodiment, the particular dosage is about 200 mg/m /day. In one embodiment, the particular dosage is about 220
• the particular dosage is about 240 mg/m /day. In one embodiment, the particular dosage is about 240 mg/m /day. In one
• the particular dosage is about 250 mg/m /day. In one embodiment, the particular dosage is about 260 mg/m /day. In one embodiment, the particular dosage is about 280
• the particular dosage is about 300 mg/ m /day. In one embodiment, the particular dosage is about 300 mg/ m /day. In one
• the particular dosage is about 320 mg/m /day. In one embodiment, the particular 2
• the dosage is about 350 mg/m /day. In one embodiment, the particular dosage is about 380
• the particular dosage is about 400 mg/m /day. In one embodiment, the particular dosage is about 450 mg/m /day. In one embodiment, the particular dosage is about 500 mg/m /day. In certain embodiments, the particular dosage is up to about 100
• the particular dosage is up to about 120 mg/m /day. In one embodiment, the particular dosage is up to about 140 mg/m /day. In one embodiment, the particular dosage is up to about 150 mg/m /day. In one embodiment, the particular dosage is up
• the particular dosage is up to about 200 mg/m /day. In one embodiment, the particular dosage is up to about 200
• the particular dosage is up to about 220 mg/m /day. In one embodiment, the particular dosage is up to about 240 mg/m /day. In one embodiment, the particular dosage is up to about 250 mg/m /day. In one embodiment, the particular dosage is up
• the particular dosage is up to about 280 mg/m /day. In one embodiment, the particular dosage is up to about 280
• the particular dosage is up to about 300 mg/ m /day. In one embodiment, the particular dosage is up to about 320 mg/m /day. In one embodiment, the particular dosage is up to about 350 mg/m /day. In one embodiment, the particular dosage is up
• the particular dosage is up to about 400
• the particular dosage is up to about 450 mg/m /day. In one embodiment, the particular dosage is up to about 500 mg/m /day. In one embodiment, the particular dosage is up to about 750 mg/m /day. In one embodiment, the particular dosage is up to about 1000 mg/m /day.
• the amount of azacitidine administered in the methods provided herein may range, e.g. , between about 5 mg/day and about 2,000 mg/day. In one embodiment, the range is between about 10 mg/day and about 2,000 mg/day. In one embodiment, the range is between about 20 mg/day and about 2,000 mg/day. In one embodiment, the range is between about 50 mg/day and about 1,000 mg/day. In one embodiment, the range is between about 100 mg/day and about 1,000 mg/day. In one embodiment, the range is between about 100 mg/day and about 500 mg/day. In one embodiment, the range is between about 150 mg/day and about 500 mg/day. In one embodiment, the range is between about 150 mg/day and about 250 mg/day.
• the particular dosage is about 10 mg/day. In one embodiment, the particular dosage is about 20 mg/day. In one embodiment, the particular dosage is about 50 mg/day. In one embodiment, the particular dosage is about 75 mg/day. In one embodiment, the particular dosage is about 100 mg/day. In one embodiment, the particular dosage is about 120 mg/day. In one embodiment, the particular dosage is about 150 mg/day. In one embodiment, the particular dosage is about 200 mg/day. In one embodiment, the particular dosage is about 250 mg/day. In one embodiment, the particular dosage is about 300 mg/day. In one embodiment, the particular dosage is about 350 mg/day. In one embodiment, the particular dosage is about 400 mg/day. In one embodiment, the particular dosage is about 450 mg/day.
• the particular dosage is about 500 mg/day. In one embodiment, the particular dosage is about 600 mg/day. In one embodiment, the particular dosage is about 700 mg/day. In one embodiment, the particular dosage is about 800 mg/day. In one embodiment, the particular dosage is about 900 mg/day. In one embodiment, the particular dosage is about 1,000 mg/day. In one embodiment, the particular dosage is about 1,200 mg/day. In one embodiment, the particular dosage is about 1,500 mg/day. In certain embodiments, the particular dosage is up to about 10 mg/day. In one embodiment, the particular dosage is up to about 20 mg/day. In one embodiment, the particular dosage is up to about 50 mg/day. In one embodiment, the particular dosage is up to about 75 mg/day. In one embodiment, the particular dosage is up to about 100 mg/day. In one embodiment, the particular dosage is up to about 500 mg/day. In one embodiment, the particular dosage is about 600 mg/day. In one embodiment, the particular dosage is about 700 mg/day. In one embodiment, the particular dosage is about 800 mg/day. In one embodiment,
• the particular dosage is up to about 120 mg/day. In one embodiment, the particular dosage is up to about 150 mg/day. In one embodiment, the particular dosage is up to about 200 mg/day,. In one embodiment, the particular dosage is up to about 250 mg/day. In one embodiment, the particular dosage is up to about 300 mg/day. In one embodiment, the particular dosage is up to about 350 mg/day. In one embodiment, the particular dosage is up to about 400 mg/day. In one embodiment, the particular dosage is up to about 450 mg/day. In one
• the particular dosage is up to about 500 mg/day. In one embodiment, the particular dosage is up to about 600 mg/day. In one embodiment, the particular dosage is up to about 700 mg/day. In one embodiment, the particular dosage is up to about 800 mg/day. In one
• the particular dosage is up to about 900 mg/day. In one embodiment, the particular dosage is up to about 1,000 mg/day. In one embodiment, the particular dosage is up to about 1,200 mg/day. In one embodiment, the particular dosage is up to about 1,500 mg/day.
• the amount of azacitidine in the pharmaceutical composition or dosage form provided herein may range, e.g. , between about 5 mg and about 2,000 mg. In one embodiment, the range is between about 10 mg and about 2,000 mg. In one embodiment, the range is between about 20 mg and about 2,000 mg. In one embodiment, the range is between about 50 mg and about 1,000 mg. In one embodiment, the range is between about 50 mg and about 500 mg. In one embodiment, the range is between about 50 mg and about 250 mg. In one embodiment, the range is between about 100 mg and about 500 mg. In one embodiment, the range is between about 150 mg and about 500 mg. In one embodiment, the range is between about 150 mg and about 250 mg. In certain embodiments, the particular amount is about 10 mg.
• the particular amount is about 20 mg. In one embodiment, the particular amount is about 50 mg. In one embodiment, the particular amount is about 75 mg. In one embodiment, the particular amount is about 100 mg. In one embodiment, the particular amount is about 120 mg. In one embodiment, the particular amount is about 150 mg. In one embodiment, the particular amount is about 200 mg. In one embodiment, the particular amount is about 250 mg. In one embodiment, the particular amount is about 300 mg. In one embodiment, the particular amount is about 350 mg. In one embodiment, the particular amount is about 400 mg. In one embodiment, the particular amount is about 450 mg. In one embodiment, the particular amount is about 500 mg. In one embodiment, the particular amount is about 600 mg. In one embodiment, the particular amount is about 700 mg.
• the particular amount is about 800 mg. In one embodiment, the particular amount is about 900 mg. In one embodiment, the particular amount is about 1,000 mg. In one embodiment, the particular amount is about 1,200 mg. In one embodiment, the particular amount is about 1,500 mg. In certain embodiments, the particular amount is up to about 10 mg. In one embodiment, the particular amount is up to about 20 mg. In one embodiment, the particular amount is up to about 50 mg. In one
• the particular amount is up to about 75 mg. In one embodiment, the particular amount is up to about 100 mg. In one embodiment, the particular amount is up to about 120 mg. In one embodiment, the particular amount is up to about 150 mg. In one embodiment, the particular amount is up to about 200 mg. In one embodiment, the particular amount is up to about 250 mg. In one embodiment, the particular amount is up to about 300 mg. In one embodiment, the particular amount is up to about 350 mg. In one embodiment, the particular amount is up to about 400 mg. In one embodiment, the particular amount is up to about 450 mg. In one embodiment, the particular amount is up to about 500 mg. In one embodiment, the particular amount is up to about 600 mg. In one embodiment, the particular amount is up to about 700 mg.
• the particular amount is up to about 800 mg. In one embodiment, the particular amount is up to about 900 mg. In one embodiment, the particular amount is up to about 1,000 mg. In one embodiment, the particular amount is up to about 1,200 mg. In one embodiment, the particular amount is up to about 1,500 mg.
• azacitidine can be delivered as a single dose such as, e.g. , a single bolus injection, or oral tablets or pills; or over time such as, e.g. , continuous infusion over time or divided bolus doses over time.
• azacitidine can be administered repetitively if necessary, for example, until the patient experiences stable disease or regression, or until the patient experiences disease progression or unacceptable toxicity. Stable disease or lack thereof is determined by methods known in the art such as evaluation of patient' s symptoms, physical examination, visualization of the tumor that has been imaged using X-ray, CAT, PET, or MRI scan and other commonly accepted evaluation modalities.
• azacitidine can be administered once daily or divided into multiple daily doses such as twice daily, three times daily, and four times daily.
• the administration can be continuous (i.e., daily for consecutive days or every day), intermittent, e.g. , in cycles (i.e. , including days, weeks, or months of rest when no drug is administered).
• azacitidine is administered daily, for example, once or more than once each day for a period of time.
• azacitidine is administered daily for an uninterrupted period of at least 7 days.
• azacitidine is administered up to 52 weeks.
• azacitidine is administered intermittently, i.e.
• azacitidine is administered for one to six days per week. In one embodiment, azacitidine is administered on alternate days. In one embodiment, azacitidine is administered in cycles (e.g. , administered daily or
• azacitidine is administered daily for two to eight consecutive weeks, then a rest period with no
• the frequency of administration ranges from about daily to about monthly
• azacitidine is administered once a day.
• azacitidine is administered twice a day.
• azacitidine is administered three times a day.
• azacitidine is administered four times a day.
• azacitidine is administered once every other day.
• azacitidine is administered twice a week.
• azacitidine is administered once every week.
• azacitidine is administered once every two weeks.
• azacitidine is administered once every three weeks.
• azacitidine is administered once every four weeks.
• azacitidine is administered once per day from one day to six months. In one embodiment, azacitidine is administered from one week to three months. In one embodiment, azacitidine is administered from one week to four weeks. In one embodiment, azacitidine is administered from one week to three weeks. In one embodiment, azacitidine is administered from one week to two weeks. In one embodiment, azacitidine is administered once per day for about one week. In one embodiment, azacitidine is administered once per day for about two weeks. In one embodiment, azacitidine is administered once per day for about three weeks. In one embodiment, azacitidine is administered once per day for about four weeks. In one embodiment, azacitidine is administered once per day for about 6 weeks. In one embodiment,
• azacitidine is administered once per day for about 9 weeks. In one embodiment, azacitidine is administered once per day for about 12 weeks. In one embodiment, azacitidine is administered once per day for about 15 weeks. In one embodiment, azacitidine is administered once per day for about 18 weeks. In one embodiment, azacitidine is administered once per day for about 21 weeks. In one embodiment, azacitidine is administered once per day for about 26 weeks. In certain embodiments, azacitidine is administered intermittently. In certain embodiment,
• azacitidine is administered intermittently in the amount of between about 50
• azacitidine is administered continuously. In certain embodiments, azacitidine is administered continuously in the amount of
• azacitidine is administered to a patient in cycles (e.g. , daily administration for one week, then a rest period with no administration for up to three weeks). Cycling therapy involves the administration of an active agent for a period of time, followed by a rest for a period of time, and repeating this sequential administration. Cycling therapy can reduce the development of resistance, avoid or reduce the side effects, and/or improves the efficacy of the treatment.
• a method provided herein comprises administering azacitidine in 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, or greater than 40 cycles.
• the median number of cycles administered in a group of patients is about 1. In one embodiment, the median number of cycles is about 2. In one embodiment, the median number of cycles is about 3. In one embodiment, the median number of cycles is about 4. In one embodiment, the median number of cycles is about 5. In one embodiment, the median number of cycles is about 6. In one embodiment, the median number of cycles is about 7. In one embodiment, the median number of cycles is about 8. In one embodiment, the median number of cycles is about 9. In one
• the median number of cycles is about 10. In one embodiment, the median number of cycles is about 11. In one embodiment, the median number of cycles is about 12. In one embodiment, the median number of cycles is about 13. In one embodiment, the median number of cycles is about 14. In one embodiment, the median number of cycles is about 15. In one embodiment, the median number of cycles is about 16. In one embodiment, the median number of cycles is about 17. In one embodiment, the median number of cycles is about 18. In one embodiment, the median number of cycles is about 19. In one embodiment, the median number of cycles is about 20. In one embodiment, the median number of cycles is about 21. In one embodiment, the median number of cycles is about 22. In one embodiment, the median number of cycles is about 23. In one embodiment, the median number of cycles is about 24.
• the median number of cycles is about 25. In one embodiment, the median number of cycles is about 26. In one embodiment, the median number of cycles is about 27. In one embodiment, the median number of cycles is about 28. In one embodiment, the median number of cycles is about 29. In one embodiment, the median number of cycles is about 30. In one embodiment, the median number of cycles is greater than about 30 cycles.
• azacitidine is administered to a patient at a dose provided herein over a cycle of 28 days which consists of a 7-day treatment period and a 21 -day resting period.
• azacitidine is administered to a patient at a dose provided herein each day from day 1 to day 7, followed with a resting period from day 8 to day 28 with no administration of azacitidine.
• azacitidine is administered to a patient in cycles, each cycle consisting of a 7-day treatment period followed with a 21-day resting period.
• azacitidine is administered to a patient at a dose of about 50, about 60, about 70, about 75, about 80, about 90, or about 100 mg/m /day, for 7 days, followed with a resting period of 21 days.
• azacitidine is administered intravenously.
• azacitidine is administered subcutaneously.
• azacitidine is administered orally in cycles.
• azacitidine is administered daily in single or divided doses for about one week.
• azacitidine is administered daily for about two weeks.
• azacitidine is administered daily for about three weeks.
• azacitidine is administered daily for about four weeks.
• azacitidine is administered daily for about five weeks.
• azacitidine is administered daily for about six weeks.
• azacitidine is administered daily for about eight weeks.
• azacitidine is administered daily for about ten weeks. In one embodiment, azacitidine is administered daily for about fifteen weeks. In one embodiment, azacitidine is administered daily for or about twenty weeks. The administration is followed by a rest period of about 1 day to about ten weeks. In one embodiment, the methods provided herein contemplate cycling treatments of about one week. In one embodiment, the methods provided herein contemplate cycling treatments of about two weeks. In one embodiment, the methods provided herein contemplate cycling treatments of about three weeks. In one embodiment, the methods provided herein contemplate cycling treatments of about four weeks. In one embodiment, the methods provided herein contemplate cycling treatments of about five weeks. In one
• the methods provided herein contemplate cycling treatments of about six weeks. In one embodiment, the methods provided herein contemplate cycling treatments of about eight weeks. In one embodiment, the methods provided herein contemplate cycling treatments of about ten weeks. In one embodiment, the methods provided herein contemplate cycling treatments of about fifteen weeks. In one embodiment, the methods provided herein contemplate cycling treatments of about twenty weeks.
• azacitidine is administered daily in single or divided doses for about one week. In one embodiment, azacitidine is administered daily for about two weeks. In one embodiment, azacitidine is administered daily for about three weeks. In one embodiment, azacitidine is administered daily for about four weeks. In one embodiment, azacitidine is administered daily for about five weeks.
• azacitidine is administered daily for about six weeks.
• the resting period of about 1, 3, 5, 7, 9, 12, 14, 16, 18, 20, 22, 24, 26, 28, 29, or 30 days.
• the rest period is 1 day.
• the rest period is 3 days.
• the rest period is 7 days.
• the rest period is 14 days.
• the rest period is 28 days.
• the frequency, number and length of dosing cycles can be increased or decreased.
• azacitidine is administered subcutaneously for 7 days. In one embodiment, azacitidine is administered on days 1-7 of each 28-day cycle. In one embodiment, 75 mg/m2/day of azacitidine is administered on days 1-7 of each 28-day cycle.
• HBG hemoglobinization assay
• KLF1 RNA by RT-qPCR
• CD235a-positive cell populations by flow cytometry (differentiation markers)
• growth and apoptosis by IncuCyte Zoom real-time imaging.
• COMPOUND 2 was used as a 10 mM stock solution in DMSO. The stock was aliquoted as 20 ⁇ batches and stored at -20 °C. The running stock was thawed and kept at room temperature in the dark for use in ongoing experiments.
• Azacitidine (AZA) was stored in a desiccator at 4 °C. The required quantity was weighed in a Mettler covered weighing balance and reconstituted in RNase and DNase free water to give a 10 mM running stock. The solution was aliquoted as 30 ⁇ batches and stored at -20 °C. A fresh 10 mM AZA vial was thawed each time for an experiment and discarded after use.
• a lOOx master stock for each compound was made fresh each time it was required from frozen stocks i.e. 100 ⁇ stock was prepared by adding 10 ⁇ of 10 mM stock in 990 ⁇ of media. From this 100X stock, the required volume was added to cells for a given desired final concentration.
• EPQ differentiation assay TFl/pLVX and TF1 IDH1 R132H cells (100,000 cells/ml) were pretreated for 7 days with COMPOUND 2, AZA or a combination (medium changed every 2 days) and washed three times with PBS to remove residual GM-CSF. Cells were then induced to differentiate using EPO (2 unit/ml) in the presence or absence of
• COMPOUND 2 Induction continued for 7 days and the cell pellets were collected and imaged for hemoglobinization content (as a surrogate for differentiation into blood lineage).
• HBG and KLFl qPCR The RNA was isolated from cells by RNAeasy kit (Qiagen) and 500 ng RNA was used to make cDNA (Superscript VILO kit, Life technologies) and followed by real-time qPCR to detect fetal hemoglobin (HBG) and KLF-1 gene expression using Taqman probes obtained from Applied Biosciences.
• FIG. 1 Cells were treated with vehicle, AZA alone, COMPOUND 2 alone, or the combination of AZA + COMPOUND 2.
• AZA AZA + COMPOUND 2.
• the sequential schedule cells were pre-treated with AZA for three days before addition of COMPOUND 2.
• the concurrent schedule cells were co-treated with AZA and COMPOUND 2 throughout the assay.
• TF- 1-IDH1 R132H cells were treated with DMSO, single agent AZA (1 ⁇ ), single agent COMPOUND 2 (0.1, 0.3 or 1.0 ⁇ ), or the combination of AZA + COMPOUND 2 at each concentration.
• Apoptosis Single agent COMPOUND 2 had no effect on induction of apoptosis. At late time points (> 60 hrs), single agent AZA increased apoptosis above the DMSO control. Cells treated with combinations of AZA+COMPOUND 2 (0.1 and 0.3 ⁇ COMPOUND 2) had greater induction of apoptosis than single agent AZA, showing potentiation.
• AML is a complex disease with a differentiation block phenotype.
• the differentiation block can be caused by mutations in genes that control cellular
• memory/epigenetic state e.g. DNMT3A, TET2, IDH1/2 and ASXL1 (Ley et al., (2010).
• DNMT3A mutations in acute myeloid leukemia N Engl J Med 363, 2424-2433; Patel et al., (2012). Prognostic relevance of integrated genetic profiling in acute myeloid leukemia. N Engl J Med 366, 1079-1089).
• the differentiation program needs to be re- wired to overcome the epigenetic effects of these founder mutations.
• COMPOUND 2 effect on HBG mRNA expression, and potentiation of AZA effect on apoptosis.
• Sequential AZA + COMPOUND 2 treatment enhanced differentiation, as shown by the increase in hemoglobinization beyond that of single agents, and greater than additive increase in mRNA expression of HBG gene.
• IDHl- mutant cancers and in particular, IDH1 R132H -mutant cancers.
• Example 2 Phase lb/2 Open-Label, Randomized Study of 2 Combinations of Isocitrate Dehydrogenase (IDH) Mutant Targeted Therapies Plus Azacitidine: Oral COMPOUND 2 Plus Subcutaneous Azacitidine and Oral COMPOUND 1 Plus SC Azacitidine in Subjects With Newly Diagnosed Acute Myeloid Leukemia Harboring an IDHl or an IDH2 Mutation, Respectively, Who Are Not Candidates to Receive Intensive
• IDH Isocitrate Dehydrogenase
• Indication Treatment of patients 18 years and older with newly diagnosed acute myeloid leukemia (AML) harboring an IDHl or an IDH2 mutation who are not candidates to receive intensive induction chemotherapy (IC).
• AML acute myeloid leukemia
• IC intensive induction chemotherapy
• COMPOUND 2 plus subcutaneous (SC) azacitidine and oral 2-methyl-l-[(4-[6- (trifluoromethyl)pyridin-2-yl] -6- ⁇ [2-(trifluoromethyl)pyridin-4-yl] amino ⁇ - 1 ,3 ,5-triazin-2- yl)amino]propan-2-ol (hereinafter COMPOUND 1) plus SC azacitidine in subjects with newly diagnosed AML harboring an IDHl or an IDH2 mutation, respectively, who are not candidates to receive intensive IC.
• SC subcutaneous
• COMPOUND 1 when administered with SC azacitidine with the suppression of 2- hydroxyglutarate (2-HG) levels in bone marrow and plasma samples.
• 2-HG 2- hydroxyglutarate
• This Phase lb/2 study is an open-label, randomized, multicenter trial to evaluate the safety and efficacy of oral COMPOUND 2 + SC azacitidine and oral COMPOUND 1 + SC azacitidine in subjects with newly diagnosed AML harboring an IDH1 or an IDH2 mutation, respectively.
• the study population consists of subjects who are not candidates to receive intensive IC.
• the study comprises a Phase lb dose-escalation stage and a Phase 2 randomized stage.
• the Phase lb stage is an open-label dose-finding study to evaluate the safety and tolerability of the combinations of oral COMPOUND 2 and oral COMPOUND 1 with SC azacitidine to define the RP2Ds of these 2 agents when administered in combination with SC azacitidine.
• the preliminary clinical activities of the oral COMPOUND 2 + SC azacitidine and the oral COMPOUND 1 + SC azacitidine regimens will also be assessed.
• the Phase lb stage consists of 3 periods: 1) screening; 2) treatment; and 3) follow-up.
• Subject screening procedures will occur during the screening period within 28 days prior to the start of study treatment.
• the diagnosis of AML with an IDH mutation will be based on local review of both hematopathology and IDH gene mutation testing of bone marrow aspirate and/or peripheral blood samples.
• Subjects eligible for enrollment must not be candidates to receive intensive IC, based on the investigator's judgment, due to the presence of comorbidities, declining performance status, or other factors.
• Subjects with newly diagnosed AML harboring an IDH1 mutation will be assigned to the oral COMPOUND 2 + SC azacitidine arm, and subjects with newly diagnosed AML harboring an IDH2 mutation will be assigned to the oral COMPOUND 1 + SC azacitidine arm.
• assignment to the oral COMPOUND 2 or COMPOUND 1 treatment arm will be based on a joint investigator and medical monitor decision and documented in the source.
• a Dose Review Team consisting of a medical monitor, lead safety physician, biostatistician, other functional area representatives or designees, as appropriate, and all active site investigators and/or designees (at sites with a subject who has received study drug), will review all adverse events (AEs) experienced by subjects during Cycle 1 of each dose level to determine whether the maximum tolerated dose (MTD) of oral COMPOUND 2 or COMPOUND 1 when administered in combination with SC azacitidine has been exceeded.
• One dose level of oral COMPOUND 2 500 mg daily
• 2 dose levels of oral COMPOUND 1 100 mg daily and 200 mg daily
• Dose levels lower than 500 mg daily for oral COMPOUND 2 and lower than 100 mg daily for oral COMPOUND 1 will be evaluated if these doses in combination with SC azacitidine are found to exceed the MTD during Cycle 1. Dose interruptions/delays and dose reductions may be used to manage toxicities. Subjects may receive study treatment until disease progression/relapse, study treatment becomes intolerable, or the subject wishes to discontinue study treatment for any reason. Response to treatment will be assessed by the investigators according to the modified International Working Group (rWG) AML Response Criteria (Cheson, et al. J Clin Oncol 2003;21(24):4642-9) . Hematologic improvement (HI) will be assessed according to the IWG myelodysplastic syndromes HI criteria (Cheson et al, Blood
• the Phase 2 stage is an open-label randomized study to evaluate the efficacy of the combinations of oral COMPOUND 2 and oral COMPOUND 1 with SC azacitidine versus SC azacitidine alone in order to assess the overall response rate (ORR), event- free survival (EFS), and morphologic complete remission (CR).
• ORR overall response rate
• EFS event- free survival
• CR morphologic complete remission
• the Phase 2 stage will also consist of 3 periods: 1) screening; 2) treatment; and 3) follow-up.
• subject screening procedures will occur during the screening period within 28 days prior to the start of study treatment, but the diagnosis of AML will be performed locally for enrollment and confirmed based on a subsequent central review.
• the IDH mutation will be assessed centrally using samples of both bone marrow aspirate and/or peripheral blood.
• Subjects eligible for enrollment are those who are not candidates to receive intensive IC, based on the investigator's judgment, due to the presence of co-morbidities, declining
• subjects with newly diagnosed AML harboring an IDH1 or IDH2 mutation will be randomized in a 2: 1 ratio to 1 of 3 arms.
• Subjects with IDH1 mutation will be randomized to receive oral COMPOUND 2 + SC azacitidine (Arm 1) versus SC azacitidine (Arm 3) in a 2: 1 ratio; and subject with IDH2 mutation will be randomized to receive oral COMPOUND 1 + SC azacitidine (Arm 2) versus SC azacitidine (Arm 3) in a 2: 1 ratio.
• Arms 1 and 2 will randomize a minimum of 50 subjects, and Arm 3 will randomize a minimum of 25 IDH1 and 25 IDH2 (50 subjects total in Arm 3) (150 subjects total in all arms).
• Study treatment will start the same day as randomization. Assessments during study treatment include efficacy, safety, HRQoL, healthcare resource utilization, pharmacokinetics, pharmacodynamics, and correlative studies.
• Dosing interruptions, dosing delays or dose modifications may occur for managing toxicities and/or augmenting treatment response during study treatment and .
• EOT End of Treatment
• eCRF electronic case report form
• the full length of the study is expected to be approximately 60 months including recruitment, screening, treatment, and follow up for Phase lb and Phase 2.
• Recruitment is expected to take 7 months for Phase lb, and 17 months for Phase 2.
• the expected duration of the Phase lb segment of the study is approximately 13 months, including a screening period for up to 28 days, and the expected duration of the Phase 2 segment of the study is approximately 25 months, including a screening period for up to 28 days.
• the End of Trial is defined as either the date of the last visit of the last subject to complete the post-treatment follow-up, or the date of receipt of the last data point from the last subject that is required for primary, secondary, and/or exploratory analysis, as pre-specified in the protocol, whichever is the later date.
• COMPOUND 2 and COMPOUND 1 are administered orally once a day (QD) on Days 1-28 of each 28-day cycle. Subjects should be instructed to take their daily dose at approximately the same time each day + 4 hours. Each dose should be taken with a glass of water and consumed over as short a time as possible. Subjects should be instructed to swallow tablets whole and to not chew the tablets. Fasting is required for 2 hours prior to and 1 hour following COMPOUND 2 or COMPOUND 1 administration. Water is allowed during fasting. [00342] Azacitidine will be administered SC for 7 days of each 28-day treatment cycle starting on Day 1 during both Phase lb and Phase 2.
• subjects randomized to the azacitidine alone arms will receive azacitidine 75 mg/m /day SC for 7 days of each 28-day cycle. All randomized subjects will receive azacitidine 75 mg/m /day SC for 7 days every 28 days until the end of the study, unless they are discontinued from the treatment. In addition, subjects may receive best supportive care as needed, including antibiotics and transfusions, per investigator discretion. In the event that 2 or fewer doses are missed during the 7-day dosing period, dosing should continue so the subject receives the full 7 days of therapy. If 3 or more days are missed during the 7-day dosing period, the investigator should contact the sponsor and a decision on dosing will be made on a case-by-case basis.
• Phase lb will use a 3 + 3 design.
• COMPOUND 2 one dose level will be explored enrolling 3 subjects.
• Cohort 1 will be initiated with oral COMPOUND 2 500 mg once a day and azacitidine 75 mg/m /day SC for 7 days of each 28-day cycle starting on Day 1 of each cycle.
• a Cohort -1 will be explored at 250 mg once a day and azacitidine 75 mg/m /day SC for 7 days of each 28-day cycle if 2 or more subjects in Cohort 1 have a dose-limiting toxicity (DLT) in Cohort 1.
• DLT dose-limiting toxicity
• Cohort 1 will be initiated with oral COMPOUND 1 100 mg once a day and azacitidine 75 mg/m /day SC for 7 days of each 28- day cycle starting on Day 1 of each cycle. If no DLTs are observed, the RP2D will be confirmed by the DRT and the 100 mg dose will be used as the starting dose for the Phase 2 segment of the study.
• Dose escalation to Cohort 2 will also be initiated with oral COMPOUND 1 200 mg once a day and azacitidine 75 mg/m /day SC for 7 days of each 28-day cycle starting on Day 1 of each cycle to explore the tolerability of the combination at this dose level.
• a Cohort -1 with oral COMPOUND 1 50 mg daily and azacitidine 75 mg/m 2 /day SC for 7 days of each 28-day cycle starting on Day 1 of each cycle will be explored if 2 or more subjects have a DLT in Cohort 1.
• Subjects with an IDH1 mutation will receive COMPOUND 2 at the RP2D orally QD on Days 1-28 of each 28-day cycle + azacitidine 75 mg/m /day SC for 7 days of each 28-day cycle.
• Subjects with an IDH2 mutation will receive COMPOUND 1 at the RP2D orally QD on Days 1-28 of each 28-day cycle + azacitidine 75 mg/m /day SC for 7 days of each 28-day cycle.
• Serial blood and bone marrow sampling will be used to determine response to therapy starting at Cycle 2. Response will be assessed locally during Phase lb. During Phase 2, response will be assessed locally and confirmed centrally according to the modified rWG criteria based on the reported hematology laboratory parameters, peripheral blood smear, bone marrow aspirates and/or biopsies, and cytogenetics.
• Subjects who discontinue study treatment prior to relapse or progression will complete monthly site visits until confirmation of relapse or progression.
• monthly follow up can be performed by site visits or phone calls. Subjects will be followed until they have died, are lost to follow up, withdraw consent for further data collection, or until study closure.
• Safety assessments include adverse events, physical examination, Eastern
• Oral COMPOUND 2 and COMPOUND 1 are dispensed on Day 1 of each treatment cycle and accounted for after completion of each treatment cycle.
• Azacitidine will be administered SC by study site personnel. Accurate recording of all IP, including preparation and dosing , will be made in the appropriate section of the subject's CRF and source documents.
• Statistical analyses in Phase lb will be primarily descriptive in nature. Tabulations will be produced for disposition, demographic and baseline disease characteristics, safety, PK, PD, and clinical activity parameters. Categorical data will be summarized by frequency distributions (numbers and percentages of subjects) and continuous data will be summarized by descriptive statistics (mean, standard deviation, median, minimum, and maximum). Data will be summarized by dose level and overall when appropriate.
• the primary efficacy endpoint of Overall Response Rate (ORR) in Phase 2 includes responses of CR, CRp, morphologic leukemia- free state [MLFS], CRi, and PR, according to modified rWG AML response criteria.
• the treatment difference in ORR will be tested using the Fisher's exact test in the ITT population. This test will provide the pivotal p-value for the comparison of the ORRs of oral COMPOUND 2 + SC azacitidine versus pooled azacitidine mono therapy group which includes subjects with IHD1 or IDH2 mutations and who are randomized to the azacitidine mono therapy , and ORRs of oral COMPOUND 1 + SC azacitidine versus pooled azacitidine mono therapy group separately.
• a maximum of 150 subjects will be randomized in this study with 50 IDH1 subjects in the oral COMPOUND 2 + SC azacitidine arm, 50 IDH2 subjects in the oral COMPOUND 1 + SC azacitidine arm, and a combined 50 IDH1 or IDH2 subjects in the azacitidine mono therapy arm (pooled azacitidine mono therapy).
• the comparisons will be conducted separately for oral COMPOUND 2 + SC azacitidine versus pooled azacitidine mono therapy and COMPOUND 1 + azacitidine versus pooled azacitidine mono therapy.
• Subject is > 18 years of age at the time of signing the informed consent form (ICF).
• Subject has previously untreated AML primary (ie, de novo) or secondary
• AML progression of MDS or myeloproliferative neoplasms ([MPN], or therapy-related) AML according to the WHO classification with > 20% leukemic blasts in the bone marrow: Have an IDH1 or IDH2 gene mutation (R132, R140, or R172); Validated local testing may be used to confirm eligibility for Phase 1, but central testing must be performed to confirm eligibility for Phase 2; By the investigator's assessment who are not candidates to receive intensive IC.
• Subject has an Eastern Cooperative Oncology Group (ECOG) performance status of 0, 1 or 2 .
• ECG Eastern Cooperative Oncology Group
• Subject has adequate organ function defined as: Serum aspartate
• AST/SGOT aminotransferase/serum glutamic oxaloacetic transaminase
• ALT/SGPT alanine aminotransferase
• GFR Cockroft-Gault glomerular filtration rate
• FCBP childbearing potential
• Females of childbearing potential may participate, providing they meet the following conditions: Agree to abstain from sexual intercourse or to use at least two effective contraceptive methods (oral, injectable, patch, or implantable hormonal contraceptive; tubal ligation; intra-uterine device; synthetic double-barrier contraceptive with spermicide; or vasectomized partner) at screening and throughout the study, and for 4 months following the last study treatment (6 months following the last dose of azacitidine in Canada); and have a negative serum ⁇ -subunit of human chorionic gonadotropin ( ⁇ -hCG) pregnancy test (sensitivity of at least 25 mlU/mL) at screening; and have a negative serum or urine (investigator's discretion under local regulations) ⁇ -hCG pregnancy test (sensitivity of at least 25 mlU/mL) within 72 hours prior to the start of study treatment in the Treatment Period (note that the screening serum pregnancy test can be used as the test prior to the start of study treatment in the Treatment
• Subject is suspected or proven to have acute promyelocytic leukemia based on morphology, immunophenotype, molecular assay, or karyotype
• Subject has AML secondary to chronic myelogenous leukemia (CML).
• CML chronic myelogenous leukemia
• Subject has received a targeted agent against an IDH1 or IDH2 mutation.
• Subject has received prior systemic anticancer therapy, HSCT, or radiotherapy for AML.
• hydroxyurea is allowed prior to the start of study treatment for the control of leukocytosis in subjects with white blood cell (WBC) counts > 30 x 10 9 /L (however, hydroxyurea should not be given within 72 hours prior to and after administration of azacitidine).
• WBC white blood cell
• hydroxyurea should not be given within 72 hours prior to and after administration of azacitidine.
• secondary AML eg, MDS or MPN
• full treatment information will be collected within the CRF.
• Subject has received prior treatment with azacitidine or decitabine for MDS .
• Subject has or is suspected of having central nervous system (CNS) leukemia.
• CNS central nervous system
• Subject has immediate life-threatening, severe complications of leukemia such as uncontrolled bleeding, pneumonia with hypoxia or shock, and/or disseminated intravascular coagulation.
• Subject has significant active cardiac disease within 6 months prior to the start of study treatment, including New York Heart Association (NYHA) class III or IV congestive heart failure; acute coronary syndrome (ACS); and/or stroke; or left ventricular ejection fraction (LVEF) ⁇ 40% by echocardiogram (ECHO) or multi-gated acquisition (MUGA) scan obtained within 28 days prior to the start of study treatment.
• NYHA New York Heart Association
• ACS acute coronary syndrome
• LVEF left ventricular ejection fraction
• ECHO echocardiogram
• MUGA multi-gated acquisition
• Subject has prior history of malignancy, other than MDS, MPN, or AML, unless the subject has been free of the disease for > 1 year prior to the start of study treatment.
• subjects with the following history/concurrent conditions are allowed: basal or squamous cell carcinoma of the skin; carcinoma in situ of the cervix; carcinoma in situ of the breast; incidental histologic finding of prostate cancer (T la or Tib using the tumor, node, metastasis clinical staging system).
• Subject is known seropositive for or has active viral infection with human
• HIV immunodeficiency virus
• HBV hepatitis B virus
• HCV hepatitis C virus
• Subject is known to have dysphagia, short-gut syndrome, gastroparesis, or other conditions that limit the ingestion or gastrointestinal absorption of drugs administered orally
• Subject has uncontrolled hypertension (systolic blood pressure [BP] > 180 mmHg or diastolic BP > 100 mmHg)
• Subject is taking the following sensitive CYP substrate medications that have a narrow therapeutic range are excluded from the study unless the subject can be transferred to other medications at least 5 half-lives prior to the start of study treatment: phenytoin (CYP2C9), S-mephenytoin (CYP2C19), thioridazine (CYP2D6), theophylline, and tizanidine (CYP1A2) .
• Subject is taking the breast cancer resistance protein (BCRP) transporter- sensitive substrate rosuvastatin; subject should be excluded from the study unless he/she can be transferred to other medications at least 5 half-lives prior to the start of study treatment
• BCRP breast cancer resistance protein
• Subject has active uncontrolled systemic fungal, bacterial, or viral infection (defined as ongoing signs/symptoms related to the infection without improvement despite appropriate antibiotics, antiviral therapy, and/or other treatment).
• Subject has known or suspected hypersensitivity to any of the components of study therapy.
• Subject is taking medications that are known to prolong the QT interval unless he/she can be transferred to other medications within > 5 half-lives prior to the start of study treatment.
• Subject has QTc interval (ie, Fridericia's correction [QTcF]) > 450 ms or other factors that increase the risk of QT prolongation or arrhythmic events (eg, heart failure, hypokalemia, family history of long QT interval syndrome) at screening.
• QTc interval ie, Fridericia's correction [QTcF]
• QTcF Fridericia's correction
• Subject has any significant medical condition, laboratory abnormality, or psychiatric illness that would prevent the subject from participating in the study.
• Subject has any condition, including the presence of laboratory abnormalities, that places the subject at unacceptable risk if he/she were to participate in the study.
• Subject has any condition that confounds the ability to interpret data from the study.
• AML patients treated with COMPOUND 2 and azacitidine, for example undergoing the clinical protocol provided herein, will show a treatment response.
• the treatment response is a Complete Response (CR), a Morphologic Leukemia-free State (MLFS), a Morphologic Complete Remission with Incomplete Neutrophil Recovery (CRi), Morphologic Complete Remission with Incomplete Platelet Recovery (CRp) , or a Partial Remission (PR), according to modified rWG AML response criteria.
• the treatment response is a hematologic improvement, for example, an
• AML patients treated with COMPOUND 2 and azacitidine in the methods provide herein will show an improvement in event-free survival (EFS), duration of response, HRQoL and/or overall survival.
• EFS event-free survival

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  • 2016-10-14 RS RS20211449A patent/RS62829B1/sr unknown
  • 2016-10-14 CA CA3002068A patent/CA3002068A1/en active Pending
  • 2016-10-14 KR KR1020187013752A patent/KR102699521B1/ko active IP Right Grant
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• 2018
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  • 2018-05-14 ZA ZA2018/03166A patent/ZA201803166B/en unknown
• 2020
  • 2020-04-13 US US16/846,976 patent/US20200405737A1/en not_active Abandoned
• 2021
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Non-Patent Citations (14)

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