WO2017026635A1 - 신규한 락토바실러스 속 미생물 및 이를 포함하는 동물 사료용 조성물 - Google Patents
신규한 락토바실러스 속 미생물 및 이를 포함하는 동물 사료용 조성물 Download PDFInfo
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- WO2017026635A1 WO2017026635A1 PCT/KR2016/005889 KR2016005889W WO2017026635A1 WO 2017026635 A1 WO2017026635 A1 WO 2017026635A1 KR 2016005889 W KR2016005889 W KR 2016005889W WO 2017026635 A1 WO2017026635 A1 WO 2017026635A1
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/16—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
- C12N1/205—Bacterial isolates
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
- C12R2001/225—Lactobacillus
Definitions
- the present invention relates to a novel Lactobacillus genus microorganisms and a composition for animal feed comprising the same.
- Microorganisms are lactic acid bacilli that are homozygous or heterozygous and are commonly found in the intestines of animals including humans and in the fermentation of dairy products or vegetables. Lactobacillus microorganisms keep the intestinal pH acidic, inhibiting the reproduction of harmful bacteria such as E. coli and Clostridium, improving diarrhea and constipation, as well as vitamin synthesis, anticancer activity, serum It is known to play a role in lowering cholesterol.
- the present inventors found out that among the Lactobacillus genus, dead cells ( ⁇ ⁇ ) of Lactobacillus rhamnosus CJLR1505 have the following effects.
- Competitive adhesion inhibitory ability with intestinal harmful bacteria contributes to the formation of intestinal flora, and the cell wall constituent eluted from the small intestine breaks down the cell wall of the intestinal tract, which causes the intestinal harmful bacteria to settle on the mesenteric membrane. To interfere.
- intestinal harmful bacteria E. coli and Salmonella are not absorbed on the surface in the intestinal environment and are washed away by the intestinal secretion and intestinal peristalsis.
- the bacterium of Lactobacillus rhamnosus CJLR1505 has triglyceride degrading ability, and when used in animal feed, it is effective for animal growth and can increase feed efficiency. In addition, there is an effect that can increase the immunity of animals ingested.
- An object of the present invention is to provide a novel lactobacillus rhamnosus CJLR1505 and a composition for animal feed containing the microorganism thereof, thereby improving the weight gain of the animal ingesting the feed containing the same and improving the anti-history.
- Lactobacillus rhamnosus CJLR1505 (KCCM11721P) is provided.
- composition for animal feed comprising Lactobacillus rhamnosus CJLR1505 (KCCM11721P), or its microorganism.
- the culture medium is indirectly heated to a temperature of 70 °C to 160 °C using a heat exchanger, and the indirectly heated culture solution Lactobacillus rhamnosus CJLR1505 (KCCM11721P) comprising rapid cooling to 10 ° C. to 60 ° C. at a rate of 10-100 L / min and separating the Lactobacillus rhamnosus CJLR1505 (KCCM11721P) microorganisms from the rapidly cooled culture broth.
- a method for producing a cell is provided.
- Lactobacillus rhamnosus CJLR1505 microorganisms according to the present invention is excellent in triglyceride degrading capacity, endotoxin adsorption capacity, pathogenic bacterial growth inhibitory ability and digestive enzyme activity, through the animal feed comprising the same to improve the weight gain of the animal and to improve the anti-potency Can be.
- Figure 1 shows an electron micrograph of the Lactobacillus rhamnosus CJLR1505 strain.
- Figure 3 is an electron micrograph showing the number of bacteria attached after intestinal epithelial cell adhesion capacity of Lactobacillus rhamnosus KCCM 32450 standard strain Lactobacillus rhamnosus CJLR1505 and Lactobacillus rhamnosus KCCM 32450.
- One embodiment of the invention is directed to Lactobacillus rhamnosus CJLR1505 (KCCM11721P).
- the present inventors collected the small intestine end of the piglets, washed with sterile distilled water, and plated onto MRS medium to which 0.001% bromphenicol purple (BCP) was added and then anaerobicly cultured at 37.
- BCP bromphenicol purple
- 47 strains of lactic acid bacteria-producing strains were selected and subcultured, followed by secondary separation of 23 species by morphological separation using these strains.
- 11 species were isolated in 3rd order.
- One strain with the best enzyme activity and triglyceride resolution was isolated.
- the isolated lactic acid bacteria producing strain was named Lactobacillius rhamnosus CJLR1505 ( Lactobacillius rhamnosus CJLR1505), and was deposited on July 8, 2015 to the Korea Microorganism Conservation Center (accession number: KCCM11721P).
- Lactobacillus rhamnosus CJLR1505 Morphological and physiological characteristics of the Lactobacillus rhamnosus CJLR1505 are shown in Table 1 below.
- Morphological characteristics (morphology when grown in MRS solid medium) -Shape of the cell Bacillus Cell polymorphism none Mobility none -Formation of apo none 2.
- Physiological Features Gram staining Gram positive Catalase voice Oxidase voice -Growth temperature and time 37 °C, 18 ⁇ 48 hours -Oxygen demand Anaerobic
- Lactobacillus rhamnosus CJLR150 has a rod shape as shown in FIG. 1 and does not form spores, and when killed, the difference between viable and dead bacteria is confirmed by glycoprotein activity on the cell wall surface.
- Lactobacillus Rhamnosus CJLR1505 No. Party kind Lactobacillus rhamnosus CJLR1505 0 Temoin - One Glycerol - 2 Erythritol - 3 D-arabinose + 4 L-arabinose - 5 D-ribose + 6 D-xylose - 7 L-xylose - 8 D-adonito - 9 Methyl- ⁇ D-xylopyranozide - 10 D-galactose + 11 D-glucose + 12 D-fructose + 13 D-mannose + 14 L-sorbose - 15 L-ramnose + 16 D-ulcitol + 17 Inositol ⁇ 18 D-mannitol + 19 D-sorbitol + 20 Methyl- ⁇ D-manopyranozide - 21 Methyl- ⁇ D-glucopyranozide - 22 N-acetylglucosamine
- Lactobacillus rhamnosus CJLR1505 (KCCM11721P) is excellent in acid resistance, bile resistance, antimicrobial activity, digestive enzyme degradation and triglyceride resolution, and when used in animal feed, it has the advantage of effectively improving the feed efficiency and animal weight gain. .
- the Lactobacillus rhamnosus CJLR1505 (KCCM11721P) of the microorganism has a high hydrophobicity and cohesiveness compared to the live bacteria has the advantage that can be attached to pathogenic microorganisms and endotoxins to improve the anti-pathology.
- composition for animal feed comprising the Lactobacillus rhamnosus CJLR1505 (KCCM11721P) or its microorganism.
- the animal feed composition may further comprise an excipient.
- the excipient is not particularly limited, and excipients commonly used in the art may be used.
- Excipients include, for example, sugars (e.g. lactose, D-mannitol, D-sorbitol, sucrose), starch (e.g. corn starch, potato starch), and inorganic salts (e.g. calcium phosphate, calcium sulfate, precipitated calcium carbonate).
- the composition for animal feed may include the Lactobacillus rhamnosus CJLR1505 (KCCM11721P) or its microorganism per 1.0g composition of 1.0 ⁇ 10 8 cfu to ⁇ 10 10 cfu. Specifically, it may be included as 1.0 ⁇ 10 8 cfu to 3.0 ⁇ 10 9 cfu, in one example may be included as 5 ⁇ 10 8 cfu.
- the microorganism When the microorganism is included in the composition in the above range, there is an advantage that the triglyceride degrading ability, endotoxin adsorption capacity, pathogenic bacterial growth inhibitory ability and digestive enzyme activity of the composition for animal feed can be maximized.
- an animal feed prepared using the animal feed composition is provided.
- the animal feed may be prepared by mixing the composition for animal feed according to one embodiment of the present invention with a conventional feed.
- Conventional feed components may include, for example, corn, soybean, soybean oil, amino acids, and the like.
- the animal feed according to another embodiment of the present invention can be prepared by further mixing the composition for another animal feed.
- the animal feed is not particularly limited and may be for feeding livestock such as cattle, pigs, horses, and the like. In one example, it may be for feeding pigs.
- Another embodiment of the present invention by culturing Lactobacillus rhamnosus CJLR1505 (KCCM11721P) to prepare a culture medium, the culture medium indirectly heated to a temperature of 70 °C to 160 °C using a heat exchanger, and the indirectly heated culture solution Lactobacillus rhamnosus CJLR1505 (KCCM11721P) comprising rapid cooling to 10 ° C. to 60 ° C. at a rate of 10-100 L / min and separating the Lactobacillus rhamnosus CJLR1505 (KCCM11721P) microorganisms from the rapidly cooled culture broth. It is about a method for producing a cell.
- the step of culturing the Lactobacillus rhamnosus CJLR1505 (KCCM11721P) to prepare a culture solution may be made using MRS agar medium.
- a culture solution may be prepared by plating Lactobacillus rhamnosus CJLR1505 (KCCM11721P) in a loop on MRS agar medium and incubating at 37 ° C. for 24 hours.
- the culture solution is indirectly heated to a temperature of 70 °C to 160 °C using a heat exchanger.
- the indirect heating may be specifically carried out to a temperature range of 80 °C to 150 °C, more specifically 90 °C to 120 °C.
- the indirectly heated culture is rapidly cooled to 10 ° C. to 60 ° C., specifically 20 ° C. to 50 ° C., in one example 4 ° C. at a rate of 10-100 L / min.
- Lactobacillus rhamnosus CJLR1505 (KCCM11721P) microorganisms can be obtained by separating the microorganisms from the rapidly cooled culture solution.
- the method for producing Lactobacillus rhamnosus CJLR1505 (KCCM11721P) microorganism may further comprise mixing a protective agent to the isolated microorganism and spray-drying it.
- the protecting agent is not particularly limited, but for example, one or more substances selected from the group consisting of yeast extract, dextrose and raw sugar can be used.
- the spray drying is also referred to as spray drying, a method of instantaneously spraying the liquid in the hot air flow to obtain a liquid dried instantaneous, such as centrifugal spraying by the rotating disk and pressurized spraying by the pressure nozzle, but is not limited thereto. to be.
- the mixing and spray-drying of the protective agent in the microbial cells may be to mix the yeast extract, dextrose and raw sugar to the microbial body to make a mixture, and then dry and powder.
- the mixed solution suspended in water for example, distilled water
- Tetrabacterial powder can be obtained.
- spray drying can be carried out, for example, with the inlet temperature of hot air being 120-200 degreeC, preferably 130-170 degreeC, and outlet temperature 30-150 degreeC, preferably 50-100 degreeC.
- the present invention is not limited thereto.
- Yeast extract, dextrose and raw sugar are based on 100 parts by weight of the mixed solution, the addition amount of yeast extract is 0.04-50 parts by weight, more specifically, 0.1-10 parts by weight, while the addition amount of dextrose is 1-100 weight To part, more specifically, 10 to 50 parts by weight, the addition amount of the raw sugar can be mixed to 0.2 to 50 parts by weight, more specifically, 0.4 to 10 parts by weight.
- the microbial powder obtained through spray drying may be mixed with inorganic salts such as calcium phosphate, calcium sulfate, calcium carbonate and the like.
- inorganic salts such as calcium phosphate, calcium sulfate, calcium carbonate and the like.
- the hygroscopicity of the inorganic salts has the advantage of controlling the water content of the microbial powder.
- the microbial powder 0.05 to 50% (w / w), specifically, 0.5 to 30%, more specifically, 0.5 to 5% and inorganic salts, for example Calcium carbonate 1-80%, specifically 5-50%. More specifically, 5 to 20% may be mixed.
- the animal feed composition to the composition while the preferred four cells at least 10 8 cfu, per 1g is 10 8 ⁇ 10 10 cfu, more preferably is good to contain 10 9 ⁇ 10 10 cfu.
- the amount of the inorganic substance is 5 to 99%, preferably 50 to 90%, more preferably 1 to 10%.
- Lactobacillus rhamnosus KCCM 32450 as a comparative strain, the safety, acid resistance, bile resistance, antimicrobial activity, digestive enzyme activity and triglyceride degradation activity of the Lactobacillus rhamnosus CJLR1505 (KCCM11721P) strain of the present invention were evaluated. It was.
- Lactobacillus rhamnosus CJLR1505 (KCCM11721P) was all negative in the gelatin liquefaction test, phenalanlan deamin test and ammonia production test, and appeared to be safe in hemolysis test.
- Lactobacillus rhamnosus CJLR1505 was less acidic than the comparative strain Lactobacillus rhamnosus KCCM 32450 at pH 2 to 4, showing excellent acid resistance.
- Lactobacillus rhamnosus CJLR1505 does not have a large decrease in the number of viable bacteria in both pH 4, 0.3% and 1% solution of bixic acid (Oxgall), and the decrease in the number of bacteria in the animal body compared to the standard strain of bile acids in the animal body It was confirmed that even if secreted, the administered lactic acid bacteria could grow.
- E. coli K88, E. coli ATCC 25922, Salmonella typhimurium KCCM 25922, Salmonella cholerasuis KCCM 10709 Three pathogens ( E. coli K88, E. coli ATCC 25922, Salmonella typhimurium KCCM 25922, Salmonella cholerasuis KCCM 10709) that were cultured in 24-hour liquid culture in TSB (Tryptic soy broth) medium (BD, USA) were homogenized with sterile swabs. Smeared at 10 5-6 cfu.
- Lactobacillus rhamnosus CJLR1505 and Lactobacillus rhamnosus KCCM 32450 were diluted to 10 9 cfu / mL in PBS buffer, respectively, and 50 ⁇ l were dispensed onto a 4 mm diameter paper disc.
- the mixture was left at room temperature until the dilution penetrated into the paper disc and incubated at 37 ° C. for 18 hours.
- the diluent is centrifuged (3,000 ⁇ g , 10 min) for the supernatant after incubation, and then used only the cells used after washing three times with PBS buffer.
- the size of the inhibitory ring was measured by the difference between the diameter of the entire transparent ring and the diameter of the agar groove. The results are shown in Table 6.
- E. coli K88 E. coli ATCC 25922 Salmonella typhimurium KCCM 25922 Salmonella cholerasuis KCCM 10709 Lactobacillus rhamnosus CJLR1505 ++ +++ +++ ++ Lactobacillus rhamnosus KCCM 32450 ++ ++ ++ ++ ++
- Lactobacillus rhamnosus CJLR1505 strain and Lactobacillus rhamnosus KCCM 32450 standard strain showed a proliferation inhibitory action against pathogenic microorganisms, but Lactobacillus rhamnosus CJLR1505 strain was Lactobacillus rhamnosus KCCM 32450 It was confirmed that the antibacterial activity is higher than the standard strain.
- Digestive enzyme digestion experiments were performed to determine whether lactic acid bacteria had enzymes capable of breaking down carbohydrates, proteins and phosphorus.
- skim milk 0.5% was added to the MRS agar medium to determine the protease activity, and methyl cellulose was added to the MRS agar medium to determine the cellulase activity. 0.2% was added and corn starch 0.2% (w / v) was added to MRS agar medium to know whether ⁇ -amylase activity was present and to know the phytase activity.
- media containing 0.5% of phytate calcium salt (Ca-phytate) were prepared. The strains isolated on each medium prepared above were streaking and observed after culturing for 24 hours.
- ⁇ -amylase and cellulase activity was treated with 2% Congo red (Sigma, USA) reagent in a culture medium for 24 hours, washed with 1M sodium chloride (Nacl), and then colored It was confirmed whether or not the enzyme.
- the determination of protease and phytase activity was confirmed by the presence of a transparent ring, and the results are shown in Table 7 below.
- Lactobacillus rhamnosus CJLR1505 strain was found to have higher digestive enzyme degradation activity compared to the Lactobacillus rhamnosus KCCM 32450 standard strain.
- Lactobacillus rhamnosus CJLR1505 can be applied to livestock to improve feed efficiency.
- the Bile salt hydrolase (hereinafter BSH) activity of Lactobacillus rhamnosus CJLR1505 was examined and the enzyme activity was confirmed by producing white precipitate in MRS solid medium containing 2 mM taurodeoxycholate hydrate (hereinafter referred to as TDCA, Sigma, USA). Precipitation was similar to that of Lactobacillus rhamnosus KCCM 32450 standard strain, a TDCA positive control strain. However, precipitation was not observed in the medium to which 2 mM sodium glycodeoxycholate (hereinafter, GDCA, Sigma, USA) was added, and the bacteria did not grow well. The results are shown in Table 8 below.
- Lactobacillus rhamnosus CJLR1505 has a high bile resistance and converts it into a form capable of degrading triglycerides by the production of BSH, which can affect the weight gain when applied to animals It was confirmed that the strain.
- Lactobacillus rhamnosus CJLR1505 (KCCM11721P) and Lactobacillus rhamnosus KCCM 32450 standard strains were each plated in a loop in MRS agar medium and incubated at 37 ° C. for 48 hours to prepare a culture solution.
- the culture solution was indirectly heated to the temperature of 100 °C using a heat exchanger, it was rapidly cooled to 10 at a rate of 30 ⁇ 100L / min.
- Lactobacillus rhamnosus CJLR1505 (KCCM11721P)
- Lactobacillus rhamnosus KCCM 32450 microorganisms were prepared by separating the microorganisms from the rapidly cooled culture.
- the intestinal epithelial cell adhesion was tested, and the hydrophobicity and cohesion of the live and dead cells of Lactobacillus rhamnosus CJLR1505 were compared, and the basic feed containing Lactobacillus rhamnosus CJLR1505 microorganisms and the same.
- HT-29 was used by Korea Cell Line Bank (KCLB), and the experimental method was Kim et. Al., Probiotic properties of Lactobacillus and Bifidobacterium strains isolated from porcine gastrointestinal tract, Applied Microbiology and Biotechnology, Volume 74, April 2007, pages 1103-1111) and Hirano et al. (Hirano et. al., The effect of Lactobacillus rhamnosus on enterohemorrhagic Escherichia coli infection of human intestinal cells in vitro, Microbiology and Immunology, Volume 47, 2003, pages 405-109). Intestinal epithelial cell adhesion experiment was performed three times using PBS buffer monolayer-forming HT-29 cells and 0.5mL RPMI1640 medium without antibiotics was added.
- KCLB Korea Cell Line Bank
- Lactobacillus rhamnosus CJLR1505 microorganisms and Lactobacillus rhamnosus KCCM 32450 microorganisms were suspended in RPMI at a concentration of about 10 9 cells / mL, inoculated in well plates, and incubated at 37 ° C for 2 hours. After cultivation was completed, the cells were washed three times using PBS buffer to remove adherent lactic acid bacteria and their ability to wash. Then, Gram staining was performed to count the number of bacteria in the microscope. It was. Experimental results are shown in FIG. 3.
- Lactobacillus ramno suspension CJLR1505 is 5.4 ⁇ 10 8 cells / are confirmed by the mL four bacterial count, 3.2 ⁇ 10 6 cells / illustrating an mL four bacterial count of Lactobacillus ramno suspension KCCM 32450 highest compared to the type strain after 2 hours It was confirmed that the epithelial cell adhesion.
- the self-agglomeration reaction was vortexed for 90 seconds by adding 1 mL of toluene to 3 mL of Lactobacillus diluted OD 600 to 0.5 against Lactobacillus rhamnosus CJLR1505 bacteria. After leaving for 1 hour in a 37 °C water bath to remove the toluene and measured the OD 600 of the aqueous layer.
- pathogenic microorganisms E. coli K88, Salmonella typhimurium KCCM 25922, Salmonella cholerasuis KCCM 10709
- live bacteria or bacteria 1: 1 (1.5 each) 1 mL of toluene was added to 3 mL of each) to vortex for 90 seconds. After leaving for 1 hour in a 37 °C
- Hydrophobicity (%) is 100 x - was calculated as (initial OD 600 1 sigan OD 600) / initial OD 600.
- the Lactobacillus rhamnosus CJLR1505 microorganism-administered group of the present invention showed an excellent effect on both weight gain and feed requirement compared to the control group not administered it.
Abstract
Description
1. 형태학적 특징(MRS 고체배지에서 성장시켰을 때의 형태) | |
- 세포의 모양 | 간균 |
- 세포의 다형성(多形性) | 없음 |
- 운동성 | 없음 |
- 아포 형성 | 없음 |
2. 생리학적 특징 | |
- 그람 염색(Gram staining) | 그람 양성(Gram positive) |
- 카탈라아제(Catalase) | 음성 |
- 옥시다아제(Oxidase) | 음성 |
- 생육 온도 및 시간 | 37℃, 18~48시간 |
- 산소 요구도 | 통성혐기성 |
No. | 당 종류 | 락토바실러스 람노서스 CJLR1505 |
0 | Temoin | - |
1 | Glycerol | - |
2 | Erythritol | - |
3 | D-arabinose | + |
4 | L-arabinose | - |
5 | D-ribose | + |
6 | D-xylose | - |
7 | L-xylose | - |
8 | D-adonito | - |
9 | Methyl-αD-xylopyranozide | - |
10 | D-galactose | + |
11 | D-glucose | + |
12 | D-fructose | + |
13 | D-mannose | + |
14 | L-sorbose | - |
15 | L-ramnose | + |
16 | D-ulcitol | + |
17 | Inositol | ± |
18 | D-mannitol | + |
19 | D-sorbitol | + |
20 | Methyl-αD-manopyranozide | - |
21 | Methyl-αD-glucopyranozide | - |
22 | N-acetylglucosamine | + |
23 | Amygdalin | + |
24 | Arbutin | + |
25 | Asculin | + |
26 | Salicin | + |
27 | D-celobiose | + |
28 | D-maltose | - |
29 | D-lactose | + |
30 | D-mellibiose | - |
31 | D-saccharose | + |
32 | D-trehalose | + |
33 | Inulin | - |
34 | D-melesitose | + |
35 | D-rafinose | + |
36 | Amidon | - |
37 | Glycogen | - |
38 | Xylitol | - |
39 | Genthiobiose | + |
40 | D-turanose | - |
41 | D-lyxose | - |
42 | D-tagatose | + |
43 | D-fucose | - |
44 | L-fucose | + |
45 | D-arabitol | - |
46 | L-arabitol | - |
47 | Gluconate | + |
48 | 2-ketogluconate | - |
49 | 5-ketogluconate | - |
균주 | 항목 | |||
젤라틴 액화 반응 검사 | 페닐알라닌 탈아민 검사 | 용혈 현상 검사 | 암모니아 생성 | |
락토바실러스 람노서스 CJLR1505 | 음성 | 음성 | α-용혈, 안전함 | 음성 |
Strains | MRS broth (pH7) | MRS broth (pH2) | MRS broth (pH3) | ||||||
생균수 (CFU/mL) | 생균수 (CFU/mL) | 생균수 (CFU/mL) | |||||||
0 h | 1 h | 3 h | 0 h | 1 h | 3 h | 0 h | 1 h | 3 h | |
락토바실러스 람노서스 CJLR1505 | 5.8 x 108 | 6.0 x 108 | 6.2 x 108 | 5.8 x 108 | 2.3 x 105 | 2.0 x 105 | 5.8 x 108 | 3.5 x 108 | 2.0 x 108 |
락토바실러스 람노서스 KCCM 32450 | 6.4 x 108 | 6.8 x 108 | 8.6 x 108 | 6.4 x 108 | 2.0 x 102 | 2.0 x 102 | 6.4 x 108 | 1.0 x 108 | 5.0 x 106 |
Strains | MRS broth (pH4) | MRS broth(pH4 and Oxgall 0.3%) | MRS broth(pH4 and Oxgall 1%) | ||||||
생균수 (CFU/mL) | 생균수 (CFU/mL) | 생균수 (CFU/mL) | |||||||
0 h | 1 h | 3 h | 0 h | 1 h | 3 h | 0 h | 1 h | 3 h | |
락토바실러스 람노서스 CJLR1505 | 1.8 x 107 | 4.2 x 107 | 1.9 x 108 | 1.8 x 107 | 1.0 x 107 | 6.8 x 106 | 1.8 x 107 | 7.2 x 106 | 2.9 x 104 |
락토바실러스 람노서스 KCCM 32450 | 1.6 x 107 | 4.6 x 107 | 6.8 x 107 | 1.6 x 107 | 6.0 x 106 | 4.2 x 104 | 1.6 x 107 | 4.6 x 104 | 2.0 x 102 |
Strains | E. coli K88 | E. coliATCC 25922 | Salmonella typhimuriumKCCM 25922 | Salmonella cholerasuisKCCM 10709 |
락토바실러스 람노서스 CJLR1505 | ++ | +++ | +++ | ++ |
락토바실러스 람노서스 KCCM 32450 | ++ | ++ | ++ | ++ |
Strains | Protease activity | Phytase activity | Lipase activity | Cellulose activity | Amlyase activity |
락토바실러스 람노서스 CJLR1505 | +++ | +++ | ++++ | ++++ | ++++ |
락토바실러스 람노서스 KCCM 32450 | ++ | ++ | +++ | +++ | +++ |
복합 담즙산/균주명 | 락토바실러스 람노서스 CJLR1505 | 락토바실러스 람노서스 KCCM 32450 |
TDCA | + | + |
GDCA | + | - |
항목/병원성 균주 | 락토바실러스 람노서스 CJLR1505 | ||
생균 | 사균화 균체 | ||
자가응집반응 | 11% | 35% | |
동시응집반응 | E. coli K88 | 45% | 87% |
Salmonella typhimuriumKCCM 25922 | 30% | 65% | |
Salmonella cholerasuisKCCM 10709 | 15% | 45% |
구분 | 락토바실러스 람노서스 CJLR1505 사균체 투여군 | 대조군 |
시험두수 | 24 | 24 |
개시 체중(kg) | 6.98 | 7.00 |
종료 체중(kg) | 18.86 | 17.76 |
일당 증체량(g) | 425 | 383 |
일당 사료 섭취량(g) | 528 | 526 |
사료 요구율 | 1.25 | 1.38 |
Claims (8)
- 락토바실러스 람노서스 CJLR1505(Lactobacillus rhamnosus CJLR1505)(KCCM11721P).
- 락토바실러스 람노서스 CJLR1505(KCCM11721P) 또는 이의 사균체를 포함하는, 동물 사료용 조성물.
- 제2항에 있어서, 부형제를 추가로 포함하는, 동물 사료용 조성물.
- 제2항 또는 제3항에 있어서, 상기 동물 사료용 조성물 1g 당 상기 락토바실러스 람노서스 CJLR1505(KCCM11721P) 사균체가 1.0×108 cfu 내지 1.0×1010 cfu로 포함되는, 동물 사료용 조성물.
- 제2항 내지 제4항 중 어느 한 항의 조성물을 포함하는 동물 사료.
- 락토바실러스 람노서스 CJLR1505(KCCM11721P)를 배양하여 배양액을 제조하고,상기 배양액을 열교환기를 이용하여 70℃ 내지 160℃의 온도로 간접 가열하고,상기 간접 가열된 배양액을 10 내지 100 L/min의 속도로 10℃ 내지 60℃로 급속 냉각하고,상기 급속 냉각된 배양액에서 락토바실러스 람노서스 CJLR1505(KCCM11721P) 사균체를 분리하는 것을 포함하는, 락토바실러스 람노서스 CJLR1505(KCCM11721P) 사균체의 제조 방법.
- 제6항에 있어서, 상기 분리된 사균체에 보호제를 혼합하고 이를 분무건조하는 것을 추가로 포함하는, 락토바실러스 람노서스 CJLR1505(KCCM11721P) 사균체의 제조 방법.
- 제7항에 있어서, 상기 보호제가 효모추출물, 덱스트로즈 및 원당으로 이루어진 군으로부터 선택되는 1종 이상의 물질인, 락토바실러스 람노서스 CJLR1505(KCCM11721P) 사균체의 제조 방법.
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MYPI2018700483A MY188407A (en) | 2015-08-12 | 2016-06-03 | Novel lactobacillus sp. microorganisms, and composition for animal feed comprising same |
CN201680046906.4A CN107949633B (zh) | 2015-08-12 | 2016-06-03 | 鼠李糖乳杆菌、动物饲料及其组合物及无活性细胞生产法 |
AU2016305391A AU2016305391B2 (en) | 2015-08-12 | 2016-06-03 | Novel Lactobacillus sp. microorganisms, and composition for animal feed comprising same |
PH12018500069A PH12018500069A1 (en) | 2015-08-12 | 2018-01-08 | Novel lactobacillus sp. microorganisms, and composition for animal feed comprising same |
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KR20150113792 | 2015-08-12 | ||
KR10-2015-0113792 | 2015-08-12 |
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CN (1) | CN107949633B (ko) |
AU (1) | AU2016305391B2 (ko) |
MY (1) | MY188407A (ko) |
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KR101873899B1 (ko) * | 2016-08-09 | 2018-07-03 | 씨제이제일제당(주) | 락토바실러스 살리바리우스 cjls1511, 상기 균 또는 이의 사균체를 포함하는 동물 사료 첨가제 조성물, 및 상기 사균체의 제조 방법 |
KR102207512B1 (ko) * | 2020-06-08 | 2021-01-27 | (주)성운파마코피아 | 항균 활성을 갖는 락토바실러스 젠세니 swpm104 |
TW202342731A (zh) * | 2021-12-23 | 2023-11-01 | 紐西蘭商恆天然合作集團有限公司 | 乳酸菌於抑制產甲烷菌生長或減少甲烷排放的應用 |
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2016
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- 2016-06-03 WO PCT/KR2016/005889 patent/WO2017026635A1/ko active Application Filing
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AU2016305391B2 (en) | 2019-01-17 |
CN107949633A (zh) | 2018-04-20 |
MY188407A (en) | 2021-12-08 |
AU2016305391A1 (en) | 2018-02-01 |
KR20170020215A (ko) | 2017-02-22 |
PH12018500069A1 (en) | 2018-07-09 |
KR101873898B1 (ko) | 2018-07-04 |
CN107949633B (zh) | 2021-04-30 |
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