WO2009113645A1 - 非荷電性親水性ブロック及び側鎖の一部に疎水性基が導入されカチオン性のポリアミノ酸ブロックを含んでなる共重合体、その使用 - Google Patents
非荷電性親水性ブロック及び側鎖の一部に疎水性基が導入されカチオン性のポリアミノ酸ブロックを含んでなる共重合体、その使用 Download PDFInfo
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- WO2009113645A1 WO2009113645A1 PCT/JP2009/054824 JP2009054824W WO2009113645A1 WO 2009113645 A1 WO2009113645 A1 WO 2009113645A1 JP 2009054824 W JP2009054824 W JP 2009054824W WO 2009113645 A1 WO2009113645 A1 WO 2009113645A1
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- group
- block copolymer
- nucleic acid
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- polyamino acid
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08G—MACROMOLECULAR COMPOUNDS OBTAINED OTHERWISE THAN BY REACTIONS ONLY INVOLVING UNSATURATED CARBON-TO-CARBON BONDS
- C08G69/00—Macromolecular compounds obtained by reactions forming a carboxylic amide link in the main chain of the macromolecule
- C08G69/02—Polyamides derived from amino-carboxylic acids or from polyamines and polycarboxylic acids
- C08G69/08—Polyamides derived from amino-carboxylic acids or from polyamines and polycarboxylic acids derived from amino-carboxylic acids
- C08G69/10—Alpha-amino-carboxylic acids
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P35/00—Antineoplastic agents
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08G—MACROMOLECULAR COMPOUNDS OBTAINED OTHERWISE THAN BY REACTIONS ONLY INVOLVING UNSATURATED CARBON-TO-CARBON BONDS
- C08G81/00—Macromolecular compounds obtained by interreacting polymers in the absence of monomers, e.g. block polymers
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
- Y10T428/00—Stock material or miscellaneous articles
- Y10T428/29—Coated or structually defined flake, particle, cell, strand, strand portion, rod, filament, macroscopic fiber or mass thereof
- Y10T428/2982—Particulate matter [e.g., sphere, flake, etc.]
Definitions
- Copolymer comprising an uncharged hydrophilic block and a cationic polyamino acid block having a hydrophobic group introduced in a part of the side chain, and its use
- the present invention comprises a block copolymer comprising a hydrophilic polymer chain block and a cationic polyamino acid chain block having a hydrophobic group introduced in a part of the side chain, as well as the copolymer and a nucleic acid molecule.
- a block copolymer comprising a hydrophilic polymer chain block and a cationic polyamino acid chain block having a hydrophobic group introduced in a part of the side chain, as well as the copolymer and a nucleic acid molecule.
- Nucleic acid drugs having physiological activity such as small interferring RNA (siRNA) and antisense oligonucleotides are expected as next-generation therapeutic agents for cancer, viral diseases and the like.
- these nucleic acids are inherently unstable in vivo, and their practical use is currently limited due to their low bioavailability.
- an efficient and safe nucleic acid delivery system that enables systemic administration is necessary (see Non-Patent Documents 1 and 2 below).
- Viral vectors are known to deliver nucleic acids to target sites with high efficiency, but their clinical use is limited by their immunogenicity and carcinogenicity (see Non-patent Documents 3 and 4 below). . For this reason, attention has been focused on non-viral vectors composed of cationic polymers and cationic lipids (see Non-Patent Documents 5 and 6 below).
- Cationic polymers form complexes with nucleic acids by electrostatic interaction, but they alone have problems with particle size control and stability in blood that can be efficiently delivered to the target.
- a method of binding a water-soluble polymer having high biocompatibility such as polyethylene glycol (PEG) has been proposed (see Patent Document 1, Patent Document 2, Patent Document 3, and Non-Patent Documents 7 and 8 below).
- PEG polyethylene glycol
- These complexes are core-shell type particles that have a hydrophilic shell made of PEG and a core to which a nucleic acid is bound. Therefore, the particle size can be controlled, but when administered intravenously. Has been reported to be quickly removed from the blood (see Non-Patent Documents 9 and 10 below), and further stabilization is desired.
- Patent Document 3 provides a graft polymer in which, for example, a PEG chain as a hydrophilic group and a palmitoyl group as a hydrophobic group are introduced via the ⁇ -amino group of the side chain of poly-L-lysine. Yes.
- a cationic polymer in which a PEG chain is introduced at one end for example, PEG® poly (N-methyljetanamine sebagate)
- cholesterol which is a hydrophobic group
- a method for stabilizing micelles has been proposed (see Non-Patent Document 11 below).
- this document does not report evaluation of blood retention, it is considered that the particles were stabilized to some extent by the introduction of hydrophobic groups.
- Patent Document 1 JP-A-8-188541
- Patent Document 2 Japanese Patent Laid-Open No. 2001-146556
- Patent Document 3 WO 99/61512
- Non-patent document 1 C. D. Novina, et al., Nature, 2004, 430, 161-164.
- Non-Patent Document 2 W. Pun, et al., Journal of Cellular Biochemistry, 2006, 98, 14-35.
- Patent Document 3 G. Crystal, et al., Science, 1995, 270, 404-410.
- Non-Patent Document 4 S. K. Tripathy, et al., Nature Medicine, 1996 2, 545-550.
- Patent Document 5 A. de Fougerolles, et al "Nature Review Drug Discovery, 2007, 6, 443-453.
- Non-Patent Document 6 S. Akhtar, et a, Journal of Clinical Investigation, 2007, 117, 3623-3632,
- Non-Patent Document 7 R. M. Schiffelers, et a and ucleic Acids Research, 2004, 32, e149.
- Patent Document 8 S. Hu-Lieskovan, et al., Cancer Research, 2005, 65, 8984-8992.
- Patent Document 9 H. K. de Wolf, et al., International Journal of Pharmaceutics, 2007, 331, 167-175.
- Non-Patent Document 11 Wang Wang, et al "Biomaterials, 2007, 28, 5358-5368.
- Patent Literature 13 C. Nicolazzi, et al., Journal of Controlled Release, 2003, 88, 429-443.
- Non-Patent Document 14 B. Thompson, et al "Bioconjugate Chemistry, 2005, 16, 608-614.
- Non-Patent Document 15 D. Oupicky, et al., Gene Therapy, 2001, 8, 713-724.
- Non-Patent Document 16 Y. Takakura, et al "International Journal of
- bagel ⁇ describes that the micelle-type particles formed from them have a certain stability, it establishes the homogeneity of the material required for medicinal materials and the homogeneity of the drug itself. Further improvements would be desirable from the standpoint of providing a system that can be easily done.
- FIG. 1 is the ' ⁇ NMR spectrum of PPLC1 synthesized in Example 2.
- a is a peak derived from the methylene group of polyethylene glycol
- b is a peak derived from the methyl group in the cholesterol group.
- Fig. 3 is a photograph obtained as a result of electrophoresis of a particle solution formed at each NZP ratio using each polymer of PPLC1 to PPLC4 and PEG-P (Lys) and siRNA (however, the NZP ratio is 0). Is a lane in which only siRNA was run.)
- the block copolymer before introducing a hydrophobic group is partly known per se, or a method known per se.
- a hydrophobic group such as a block copolymer in which B in the above formula is a hydrogen atom
- B in the above formula is a hydrogen atom
- the block copolymer that can be used in the present invention is a known or commercially available polymer having an uncharged hydrophilic polymer chain and a polymer having a cationic polyamino acid chain, or if necessary. After purification to narrow the molecular weight distribution, it can be produced by re-coupling by a method known per se.
- these may be present in the same molecule.
- Mino squeeze size 3 5— Di methyl— 1— pyrazoly ormaminiai um
- a method of reacting O-methylisourea in the presence of a base such as ammonium hydroxide can be used. These reactions can be performed either before or after introducing the hydrophobic group into B. Since the block copolymer of the present invention that can be provided in this way has a cationically charged group, it can form an ion complex with, for example, an anion charged compound, a nucleic acid, or an anion charged drug. Such ion complexes can autonomously associate in an aqueous medium to form micelles, so-called polymer micelles.
- Such a micelle can have a core-shell structure in which a nucleic acid or a drug is encapsulated in a core part and an uncharged hydrophilic polymer chain is included as a shell part. Both blocks
- the nucleotide contained in the nucleic acid may be a natural type or a non-natural type that has been chemically modified, and a molecule to which an amino group, a thiol group, a fluorescent compound, or the like is added. Also good. Without limitation, force ⁇ , the nucleic acid may consist of 4 to 20,000 bases, preferably 10 to "!! 0, 000 bases, more preferably 18 to 30 bases.
- siRNA is a target gene or polynucleotide
- siRNA can be either single-stranded or double-stranded with a length of about 18-30 nucleotides.
- specific examples of siRNA include genes that can be targeted for gene therapy. three Such genes include, but are not limited to, BCL-2 and Crohn's disease related to PKC and malignant melanoma related to non-small cell lung cancer, etc.
- ICAM-1 related to HCV related to hepatitis C TN F related to rheumatoid arthritis or psoriasis, related to asthma C- raf ki nase related to adenosine AI receptor, ovarian cancer, H- ras related to spleen cancer, c-myc related to coronary artery disease, P KA related to colorectal cancer Ria, HIV related to AIDS, DNA methyltransferase related to solid cancer, VEGF receptor related to cancer, ribonucleotide reductase related to kidney cancer, CMV IE2 related to CMV retinitis MM P-9 related to prostate cancer, TGF jS 2 related to malignant glioma, CD49d related to multiple sclerosis, PTP-1 B related to diabetes, cancer related c One can include myb, EGFR related to breast cancer, mdrl, autotaxin and GLUT-1 genes related to cancer.
- antisense nucleic acid those known in the art or all having the same function or action as those can be used. Such functions form a hybrid to mRNA and form a double-stranded molecule that inhibits gene translation.
- ribozyme refers to an RNA molecule that has the ability to specifically degrade internal and other single-stranded RNA as well as DNA restriction endonuclease.
- a method for forming a complex of the block copolymer of the present invention and a nucleic acid a method of reducing the content of the polar organic solvent in the aqueous solution containing the polar organic solvent in which the block copolymer and the nucleic acid are dissolved is used. be able to.
- the method for reducing the content of the polar organic solvent in the aqueous solution containing the polar organic solvent is not limited, but a method by dialysis or distillation, or ultrafiltration if necessary after dilution with an aqueous solution. And the like.
- the block copolymer and nucleic acid used to form the complex of the present invention are defined by [molar concentration of cation residue in block copolymer] Z [molar concentration of phosphate group in nucleic acid] NZ P is preferably mixed so that the specific force is 1 to 50, more preferably 2 to 20, and still more preferably 4 to 10.
- the number of moles of the substituent represented by 2 the number of moles of the substituent represented by Formula IV, the number of moles of the hydrophobic substituent bonded to the polyamino acid block via NH— in B, It can be calculated from the sum total of the number of moles of hydrophobic substituents bonded to the polyamino acid block via the substituent represented by the formula ⁇ .
- the complex of the block copolymer and the nucleic acid of the present invention is preferably a particle having an average particle diameter of 10 to 300 nm, more preferably an average particle diameter of 20 nm to 200 nm.
- the complex of the block copolymer of the present invention and the nucleic acid preferably has a zeta potential of 10 to 10 mV. More preferably, it is _5-5mV.
- a complex of a block copolymer and a nucleic acid has the following characteristics and advantages.
- Example 1 Synthesis of polyethylene glycol monopoly (L monolysine) block copolymer and introduction of cholesterol group into the polymer
- Example 2 Polyethylene glycol monopoly (L-lysine) block copolymer
- Example 3 Polyethylene glycol monopoly (L-lysine) block copolymer
- N-succinimidyl stearate Drying under reduced pressure gave N-succinimidyl stearate.
- PPLS1 was analyzed by 1 H-NMR (see Fig. 2 for the spectrum).
- the introduction rate of stearoyl group was calculated from the intensity ratio of the peak of methyl group proton (CH 3 : 0.8 ppm) and PEG methylene group proton (OCH 2 CH 2 : 3.5 ppm).
- a stearoyl group was introduced in 40% of the total recycle.
- Mw weight average molecular weight 12000 a-methoxy mono- ⁇ -aminopoly (ethylene glycol) (NOF Corporation) 1. Dissolve 5g in dimethyl sulfoxide 22,5ml and add ⁇ -benzyloxycarbonyl _L-lysine ⁇ —Carboxylic anhydride (NCA) 1.03 g (27 equivalents to polyethylene glycol) and trifluoroacetyl mono-L-lysine N-force rubonic anhydride (NCA) 603 mg (18% to polyethylene glycol) (Equal volume) of dimethyl sulfoxide solution (24.5 ml) was added and the reaction was carried out at 35 ° C for 48 hours.
- NCA Carboxylic anhydride
- NCA trifluoroacetyl mono-L-lysine N-force rubonic anhydride
- Polymerized poly (ethylene glycol) monoblock monopoly one monobenzyloxycarbonyl one L monolysine ZL—lysine) (PEG— P (Lys (Z) ZLys) hydrochloride 2.
- Example 5 Synthesis of polyethylene glycol-poly (L-lysine-1 ⁇ -benzyloxycarbonyl) -poly (L-lysine) block copolymer
- Mw weight average molecular weight 1 2000 1-methyloxy-OJ-aminopoly (ethylene glycol) (NOF Corporation) 1.5g was dissolved in 22.5ml of dimethyl sulfoxide, and ⁇ -benzyloxycarbonyl 1.
- NCA L-Lysine ⁇ -carboxylic anhydride
- N-carboxylic anhydride N-carboxylic anhydride of trifluoroacetyl mono-L-lysine (1 against polyethylene glycol)
- Tylene glycol poly ( ⁇ -benzyloxycarbonyl-1-L-lysine), and poly
- the siRNA used in this example was designed to target the cassava luciferase gene, using 5′-CUUACGCUGAGUACUUCGAdTd T-3 ′ as the sense strand and 5′-UCGAAGUACUCAGCGUAAGdTdT-3 ′ as the antisense strand
- a double chain was formed by a conventional method, and the product was purchased after requesting synthesis from Nippon Easy Co., Ltd.
- the particle solution was diluted with 1 OmM Tris HCI buffer at pH 7.4 or concentrated with a centrifugal filtration unit (Amicon Ultra, Millipore) to adjust the concentration appropriately.
- Figure 3 shows the results. When siRNA is incorporated into particles, the mobility in electrophoresis is reduced, and the free siRNA-derived band disappears. In all polymers, when the NZP ratio was 1.5 or more, no free siRNA-derived band could be confirmed, and almost all siRNA was incorporated into the particles.
- each particle solution was prepared to have a siRNA concentration of 5 M.
- the cumulant average particle size was 40 to 1 OOnm.
- Figure 6 shows the histogram of the diameter distribution.
- Example 7 Characterization by zeta potential measurement
- Fig. 7 shows the results of measuring the zeta potential of the formed particles. 1 odor compared to NZP
- PEG layer is present, and the charge inside the particle is almost completely shielded.
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- Other Resins Obtained By Reactions Not Involving Carbon-To-Carbon Unsaturated Bonds (AREA)
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Priority Applications (6)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN2009801084707A CN101970541B (zh) | 2008-03-10 | 2009-03-06 | 包含非荷电性亲水性嵌段以及侧链的一部分引入了疏水性基团的阳离子性聚氨基酸嵌段的共聚物、及其使用 |
US12/922,116 US8431545B2 (en) | 2008-03-10 | 2009-03-06 | Copolymer including uncharged hydrophilic block and cationic polyamino acid block having hydrophobic group in part of side chains, and use thereof |
AU2009224286A AU2009224286B2 (en) | 2008-03-10 | 2009-03-06 | Copolymer including uncharged hydrophilic block and cationic polyamino acid block having hydrophobic group in part of side chains, and use thereof |
CA2718003A CA2718003C (en) | 2008-03-10 | 2009-03-06 | Copolymer including uncharged hydrophilic block and cationic polyamino acid block having hydrophobic group in part of side chains, and use thereof |
EP09720268.3A EP2272897B1 (en) | 2008-03-10 | 2009-03-06 | Copolymer including uncharged hydrophilic block and cationic polyamino acid block having lateral chain to which hydrophobic radical is partially introduced, and use of copolymer |
JP2010502885A JP5467366B2 (ja) | 2008-03-10 | 2009-03-06 | 非荷電性親水性ブロック及び側鎖の一部に疎水性基が導入されたカチオン性のポリアミノ酸ブロックを含んでなる共重合体、その使用 |
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JP2008059886 | 2008-03-10 | ||
JP2008-059886 | 2008-03-10 |
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US (1) | US8431545B2 (ja) |
EP (1) | EP2272897B1 (ja) |
JP (1) | JP5467366B2 (ja) |
KR (1) | KR101564796B1 (ja) |
CN (1) | CN101970541B (ja) |
AU (1) | AU2009224286B2 (ja) |
CA (1) | CA2718003C (ja) |
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Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPH08188541A (ja) * | 1995-01-10 | 1996-07-23 | Res Dev Corp Of Japan | 静電結合型高分子ミセル薬物担体とその薬剤 |
JP2001146556A (ja) * | 1999-11-19 | 2001-05-29 | Nano Career Kk | コア−シェル構造のポリイオンコンプレックスミセル |
WO2004105799A1 (ja) * | 2003-05-29 | 2004-12-09 | Toudai Tlo, Ltd. | 安定化高分子ミセル |
WO2007043486A1 (ja) * | 2005-10-05 | 2007-04-19 | Tokyo Cro, Inc. | 生体適合性ブロック共重合体、その用途および製造法 |
WO2008047948A1 (fr) * | 2006-10-19 | 2008-04-24 | Nanocarrier Co., Ltd. | Copolymère bloc pour complexe médicamenteux et composition pharmaceutique |
Family Cites Families (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
GB9811059D0 (en) | 1998-05-23 | 1998-07-22 | Univ Strathclyde | Polyamino acid vesicles |
JP4535229B2 (ja) | 2003-05-08 | 2010-09-01 | 国立大学法人 東京大学 | ポリエチレングリコール−ポリカチオンブロック共重合体 |
EP1859812B1 (en) * | 2005-02-10 | 2014-01-01 | The University of Tokyo | Polycation chargeable polymer and use as carrier of nucleic acid |
WO2006090924A1 (ja) * | 2005-02-28 | 2006-08-31 | The University Of Tokyo | ペプチドリガンドを有するブロック共重合体 |
-
2009
- 2009-03-06 EP EP09720268.3A patent/EP2272897B1/en not_active Not-in-force
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Patent Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPH08188541A (ja) * | 1995-01-10 | 1996-07-23 | Res Dev Corp Of Japan | 静電結合型高分子ミセル薬物担体とその薬剤 |
JP2001146556A (ja) * | 1999-11-19 | 2001-05-29 | Nano Career Kk | コア−シェル構造のポリイオンコンプレックスミセル |
WO2004105799A1 (ja) * | 2003-05-29 | 2004-12-09 | Toudai Tlo, Ltd. | 安定化高分子ミセル |
WO2007043486A1 (ja) * | 2005-10-05 | 2007-04-19 | Tokyo Cro, Inc. | 生体適合性ブロック共重合体、その用途および製造法 |
WO2008047948A1 (fr) * | 2006-10-19 | 2008-04-24 | Nanocarrier Co., Ltd. | Copolymère bloc pour complexe médicamenteux et composition pharmaceutique |
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Also Published As
Publication number | Publication date |
---|---|
EP2272897B1 (en) | 2016-12-28 |
CA2718003C (en) | 2016-05-03 |
EP2272897A4 (en) | 2012-01-25 |
CN101970541A (zh) | 2011-02-09 |
JPWO2009113645A1 (ja) | 2011-07-21 |
AU2009224286B2 (en) | 2014-06-12 |
KR101564796B1 (ko) | 2015-10-30 |
EP2272897A1 (en) | 2011-01-12 |
CA2718003A1 (en) | 2009-09-17 |
TW200948384A (en) | 2009-12-01 |
WO2009113645A8 (ja) | 2010-11-18 |
KR20100138910A (ko) | 2010-12-31 |
US20110052917A1 (en) | 2011-03-03 |
CN101970541B (zh) | 2013-12-04 |
AU2009224286A1 (en) | 2009-09-17 |
US8431545B2 (en) | 2013-04-30 |
TWI459968B (zh) | 2014-11-11 |
JP5467366B2 (ja) | 2014-04-09 |
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