WO2009088032A1 - PcrVに対する抗体 - Google Patents
PcrVに対する抗体 Download PDFInfo
- Publication number
- WO2009088032A1 WO2009088032A1 PCT/JP2009/050118 JP2009050118W WO2009088032A1 WO 2009088032 A1 WO2009088032 A1 WO 2009088032A1 JP 2009050118 W JP2009050118 W JP 2009050118W WO 2009088032 A1 WO2009088032 A1 WO 2009088032A1
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- seq
- pcrv
- monoclonal antibody
- antibody
- amino acid
- Prior art date
Links
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
- C07K16/12—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from bacteria
- C07K16/1203—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from bacteria from Gram-negative bacteria
- C07K16/1214—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from bacteria from Gram-negative bacteria from Pseudomonadaceae (F)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/04—Antibacterial agents
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P37/00—Drugs for immunological or allergic disorders
- A61P37/02—Immunomodulators
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/70—Immunoglobulins specific features characterized by effect upon binding to a cell or to an antigen
- C07K2317/76—Antagonist effect on antigen, e.g. neutralization or inhibition of binding
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/90—Immunoglobulins specific features characterized by (pharmaco)kinetic aspects or by stability of the immunoglobulin
- C07K2317/92—Affinity (KD), association rate (Ka), dissociation rate (Kd) or EC50 value
Definitions
- the present invention relates to a monoclonal antibody that recognizes PcrV or a part thereof. Specifically, the present invention relates to an antibody having a higher neutralizing activity (hereinafter also referred to as cytotoxicity inhibiting activity) than conventional anti-PcrV antibodies or a part thereof, and a pharmaceutical composition containing them.
- cytotoxicity inhibiting activity an antibody having a higher neutralizing activity (hereinafter also referred to as cytotoxicity inhibiting activity) than conventional anti-PcrV antibodies or a part thereof, and a pharmaceutical composition containing them.
- Pseudomonas aeruginosa is an obligately aerobic gram-negative bacilli that exists widely in nature. Although it is usually low in pathogenicity, it is a pathogenic bacterium that causes opportunistic infections that frequently occur in patients with various basic diseases such as cancer and diabetes, and in patients receiving immunosuppressive drugs. Often causes serious consequences, such as tract infections and sepsis. Pseudomonas aeruginosa infection is currently considered one of the most difficult to treat in the clinical setting. This is because Pseudomonas aeruginosa is not only less sensitive to existing antibiotics but also has a strong tendency to easily acquire resistance to various antibiotics and become intractable. Thus, for Pseudomonas aeruginosa, there is a limit in the response of developing new antibiotics one after another, and a treatment method that does not depend on antibiotics is eagerly desired.
- PcrV is a constituent protein of the type III exotoxin secretion system consisting of 294 residues (NCBI Accession No. AAC45935, SEQ ID NO: 1), and the operon sequence encoded is disclosed (Patent Document 1, Non-Patent Document 1). ).
- Non-Patent Document 2 Since control over PcrV may lead to therapeutic measures in Pseudomonas aeruginosa infection (Non-Patent Document 2), polyclonal antibodies (Non-Patent Documents 3 and 4) and monoclonal antibodies (Non-Patent Documents 3 and 4) having neutralizing activity Document 2, Non-Patent Documents 5 and 6) have been reported.
- humanization is difficult with polyclonal antibodies, and antigenicity is difficult to improve, making it difficult to use as a pharmaceutical composition.
- the problem to be solved by the present invention is to provide an effective means for treating infections, particularly infections caused by Pseudomonas aeruginosa.
- the present invention (1) (A) inhibits 50% or more of the cytotoxic activity against Pseudomonas aeruginosa white blood cells at a concentration of 1 nM to 200 nM in vitro; (B) inhibits more than 50% of the cytotoxic activity of Pseudomonas aeruginosa on myeloma cells at a concentration of 1 nM to 50 nM in vitro; and (C) The dissociation constant (Kd) with PcrV is 2 ⁇ 10 ⁇ 9 (M) or less.
- the monoclonal antibody of the present invention or a part thereof has a very excellent neutralizing activity against PcrV, it is useful as a therapeutic agent for infectious diseases.
- FIG. 1 shows a displacement curve of biotin-labeled PcrV with unlabeled PcrV in PcrV antibodies (1F3, 2A4, 6F5, 7A7 and Mab166).
- FIG. 2 shows the affinity of PcrV antibodies (1F3, 2A4, 6F5, 7A7 and Mab166) by surface plasmon resonance analysis.
- FIG. 3 shows the results of a sandwich assay between PcrV antibodies (1F3, 2A4, 6F5, 7A7, Mab166) and Mab166.
- FIG. 4 shows the inhibitory effect of PcrV antibodies (1F3, 2A4, 6F5, 7A7 and Mab166) on the cytotoxic activity of Pseudomonas aeruginosa SR24 strain on U937 cells.
- FIG. 5 shows the inhibitory effect of PcrV antibodies (1F3, 2A4, 6F5, 7A7 and Mab166) on the cytotoxic activity of Pseudomonas aeruginosa SR24 strain on myeloma cells P3U1.
- FIG. 6 shows a replacement curve of biotin-labeled PcrV with unlabeled full-length PcrV and deletion mutation PcrV in PcrV antibodies (1F3, 2A4, 9D12, 12H9 and m166).
- FIG. 7 shows the reactivity of full length PcrV and deletion mutant PcrV in PcrV antibodies (1F3, 2A4, 9D12, 12H9 and m166) in Western blot.
- FIG. 6 shows a replacement curve of biotin-labeled PcrV with unlabeled full-length PcrV and deletion mutation PcrV in PcrV antibodies (1F3, 2A4, 9D12, 12H9 and m166).
- FIG. 8 shows the correlation between the antibody and full-length PcrV by suppressing the cytotoxicity inhibiting activity.
- FIG. 9 shows the correlation between the antibody and the deletion mutant PcrV by suppressing the cytotoxicity inhibiting activity.
- 1 shows the amino acid sequence of the variable region of the 1F3 antibody. The underlined portion indicates the CDR region. The amino acid sequence of the variable region of 2A4 antibody is shown. The underlined portion indicates the CDR region.
- the “monoclonal antibody” targeted by the present invention is a monoclonal antibody that specifically binds to the aforementioned PcrV. More specifically, (1) inhibits 50% or more of the cytotoxic activity against Pseudomonas aeruginosa white blood cells at a concentration of 1 nM to 200 nM in vitro. (2) inhibits more than 50% of the cytotoxic activity of Pseudomonas aeruginosa on myeloma cells at a concentration of 1 nM to 50 nM in vitro; and (3) The dissociation constant (Kd) with PcrV is 2 ⁇ 10 ⁇ 9 (M) or less.
- a monoclonal antibody against PcrV having at least one property selected from:
- the monoclonal antibody of the present invention has a strong cytotoxic inhibitory activity.
- the inhibitory activity of Pseudomonas aeruginosa has a 50% or higher inhibition at a concentration in the range of 1 to 200 nM, preferably 2 to 100 nM, more preferably 5 to 25 nM (medium Sum) activity.
- the inhibitory activity of inhibiting the cytotoxic activity of Pseudomonas aeruginosa by 50% or more at a concentration within the range of 1 to 50 nM, preferably 2 to 30 nM, more preferably 4 to 20 nM. have. These values are from Dara W. This greatly exceeds the activity value of Mab166 reported in Frank et al. (J. Infect. Disease, 2002, 186, 64).
- the monoclonal antibody of the present invention is characterized by having the epitope in the region from position 136 to position 233 in the full-length amino acid sequence of PcrV (SEQ ID NO: 1).
- the present inventors have found that an antibody recognizing this region has a stronger activity (cytotoxic inhibition activity) than an antibody recognizing other regions.
- Identification of the recognition epitope of the monoclonal antibody can be performed as follows. First, various partial structures of molecules recognized by monoclonal antibodies are prepared. In preparing the partial structure, a method for preparing various partial peptides of the molecule using a known oligopeptide synthesis technique, or a DNA sequence encoding the target partial peptide using a gene recombination technique as a suitable expression plasmid. There are methods for integration and production inside and outside the host such as Escherichia coli, but for the above purpose, it is common to use both in combination.
- the corresponding oligopeptides of the corresponding portion or variants of the peptides are synthesized in various ways using oligopeptide synthesis techniques well known to those skilled in the art, and the preventive or therapeutic agent of the present invention is contained as an active ingredient.
- the epitopes are limited by examining the binding properties of the monoclonal antibodies to those peptides, or by examining the competitive inhibitory activity of the peptides on the binding of the monoclonal antibody to the antigen.
- commercially available kits for example, SPOTs kit (Genosis Biotechnology), a series of multi-pin peptide synthesis kits using multi-pin synthesis method, etc.can also be used.
- Cytotoxicity inhibitory activity can be measured by the following procedure. First, a monoclonal antibody which is a measurement target of cytotoxicity inhibitory activity is diluted with a 2-fold dilution series and adjusted to an appropriate concentration. Next, cells affected by the toxin of Pseudomonas aeruginosa (hereinafter referred to as target cells) are diluted with a cell culture medium or the like and adjusted to an appropriate number. Specifically, when using myeloma cells, adjust in the range of 3 ⁇ 10 6 to 5 ⁇ 10 6 Cells / ml, and when using white blood cells, adjust in the range of 1 ⁇ 10 6 to 3 ⁇ 10 6 Cells / ml. It is desirable.
- Pseudomonas aeruginosa is adjusted to 1 ⁇ 10 7 to 1 ⁇ 10 8 cfu / ml with a culture medium or the like. Then, in the presence of the monoclonal antibody described above, Pseudomonas aeruginosa and target cells are cultured under appropriate culture conditions in the same test tube and well (for example, in vitro conditions such as wells on a microplate).
- the culture conditions at this time may be general culture conditions that are favorable for growing cells and bacteria.
- the optimal conditions for the culture time are appropriately changed depending on the type of target cell. For example, about 1 to 3 hours are preferable when using myeloma cells, and about 1 to 3 hours when using white blood cells.
- a concentration that inhibits 50% of the control group (effective concentration) is calculated.
- concentration concentration that inhibits 50% of the control group (effective concentration) is calculated.
- Various methods have been established for determining whether a target cell is alive or not. After adding a color reagent, measurement of absorbance at an appropriate wavelength (for example, 400 to 500 nm) is useful (Reference: Nature). Medicine, 1999, vol. 5, pp. 392-395).
- the dissociation constant (Kd) used as an index representing the affinity of the monoclonal antibody for the antigen can be analyzed according to various methods. For example, using the Scatchard method using antigens labeled with various labeling agents, BiacoreX (manufactured by Amersham Pharmacia) or a similar kit, which is a commercially available measurement kit, the instruction manual and the experimental operation method attached to the kit Can be easily analyzed.
- the dissociation constant (Kd value) obtained using these methods is expressed in units of M (mol). The tested monoclonal antibody shows that the smaller the dissociation constant, the stronger the affinity.
- the dissociation constant (Kd) with PcrV is 2 ⁇ 10 ⁇ 9 (M) or less, preferably 1.5 ⁇ 10 ⁇ 9 (M) or less, more preferably 1.2 ⁇ 10 ⁇ 9 (M) or less.
- variable regions of the heavy and light chains are derived from humans, for example, variants of SEQ ID NOs: 11 to 14 (for example, substitution or insertion of one or several amino acids, addition or addition) Including, but not limited to, those containing deletions).
- the constant region domains are also preferably suitable human constant region domains, such as Kabat E. A. Et al. , US Department of Health and Human Services, Public Health Service, and National Institute of Health.
- amino acid sequence of the variable region to the antibody amino acid sequence database ([Sequence (of Proteins of Immunological Interest] US Dept.Health and Human Services, 1983) created by Kabat et al. Can be found.
- the CDR region sequence may be modified by at least one addition / insertion, substitution or deletion as long as the desired biological activity (for example, binding activity or neutralization activity) of the present invention is maintained. It is included in the present invention. Examples thereof include sequences having a homology with each CDR region of 90 to 100%. Preferable examples include sequences having a homology of 95 to 100%. More preferred is a sequence having a homology of 98 to 100%.
- the framework region can be related to any kind of framework region, but is preferably of human origin.
- a suitable framework area is Kabat E.I. A. These documents can be selected by referring to them.
- a preferred heavy chain framework is a human heavy chain framework, such as the anti-PcrV antibody framework shown in FIG. 10 or FIG. It can be determined from the sequence shown in FIG. 10 or FIG. 11 with reference to the above document. In the same manner, the anti-PcrV light chain framework can also be determined from the sequence shown in FIG.
- CDR1 comprises the amino acid sequence SFTSYWMH (SEQ ID NO: 15).
- CDR2 has the amino acid sequence INPSNGRTNYNEKFNT (SEQ ID NO: 16),
- CDR3 has the amino acid sequence YGNYVVYYTMDY (SEQ ID NO: 17), an immunoglobulin heavy chain variable region (V H ) or fragment thereof and (ii) a human heavy chain And an antibody containing a constant part thereof or a fragment thereof.
- CDR1 has the amino acid sequence SASSVSYME (SEQ ID NO: 18), CDR2 has the amino acid sequence TTSKLAS (SEQ ID NO: 19), and CDR3 has the amino acid sequence HQWRNYPFT (SEQ ID NO: 20). It preferably contains the chain variable region (V L ) and (ii) the constant part of a human light chain or a fragment thereof.
- CDR1, CDR2 and CDR3, which are complementarity determining regions are included in the sequence, in which case CDR1 has the amino acid sequence SITSDYAWN (SEQ ID NO: 21).
- An immunoglobulin heavy chain variable region (V H ) or fragment thereof, and (ii) human, having the amino acid sequence YTYNGDTSYNPSLKS (SEQ ID NO: 22) and CDR3 having the amino acid sequence SRNYYGAWFAY (SEQ ID NO: 23) Mention may be made of antibodies comprising the constant part of the heavy chain or fragments thereof.
- CDR1 has the amino acid sequence KASQYVGGTTVA (SEQ ID NO: 24)
- CDR2 has the amino acid sequence RASTRHT (SEQ ID NO: 25)
- CDR3 has the amino acid sequence QQYCSSPLT (SEQ ID NO: 26). It preferably contains the chain variable region (V L ) and (ii) the constant part of a human light chain or a fragment thereof.
- the monoclonal antibody of the present invention can be prepared by an existing general production method using PcrV (natural, recombinant, synthetic, etc.) as an immunogen.
- mammals preferably mice, rats, hamsters, guinea pigs, rabbits, cats, dogs, pigs, goats, sheep, donkeys, horses, with PcrV as an immunogen and optionally with Freund's adjuvant.
- bovine including transgenic animals created to produce antibodies from other animals such as human antibody-producing transgenic mice
- muscle Immunization is performed by injecting once or several times into the body, veins, food pads or abdominal cavity.
- immunization is performed 1 to 4 times about every 1 to 21 days from the initial immunization, and antibody-producing cells can be obtained from the immunized mammal about 1 to 10 days after the final immunization.
- the number of times of immunization and the time interval can be appropriately changed depending on the nature of the immunogen used.
- PcrV used as an immunogen or hapten include the following.
- A Cells that express PcrV on the cell surface or cell lines that are artificially established to express on the cell surface, or genetically modified cells that are created using genetic recombination technology to express PcrV on the cell surface ;
- B A culture supernatant obtained by culturing a genetically modified cell prepared using a gene recombination technique so that PcrV or a part thereof is expressed as a protein, or PcrV purified from the culture supernatant Part; or (c) chemically synthesized PcrV or part thereof.
- a hybridoma secreting a monoclonal antibody can be prepared according to the method of Kohler and Milstein et al. (Nature, 1975, vol. 256, p495 to 497) and a method analogous thereto. That is, antibody-producing cells contained in the spleen, lymph node, bone marrow, tonsil, etc., preferably from the spleen obtained from the immunized mammal as described above, and preferably mouse, rat, guinea pig, hamster, rabbit or human
- a hybridoma can be prepared by cell fusion of a myeloma cell having no autoantibody-producing ability derived from a mammal such as a mouse, rat or human.
- myeloma cells used for cell fusion cell lines generally obtained from mice, such as 8-azaguanine-resistant mice (derived from BALB / c) myeloma strain P3X63Ag8U. 1 (P3-U1) [Yelton, D. E. Et al. Current Topics in Microbiology and Immunology, 81, 1-7 (1978)], P3 / NSI / 1-Ag4-1 (NS-1) [Kohler, G. Et al. European J. Immunology, 6, 511-519 (1976), Sp2 / O-Ag14 (SP-2) [Shulman, SM. Et al.
- the screening of hybridoma clones producing monoclonal antibodies is carried out by cultivating the hybridomas in, for example, a microtiter plate, and determining the reactivity of the culture supernatant of the wells in which proliferation has been observed with the immunogen used in the above-described mouse immunization.
- the measurement is performed by an immunoassay method such as RIA or ELISA, and a clone producing a monoclonal antibody exhibiting specific affinity for the immunogen or hapten is selected.
- a non-competitive ELISA is used in which an immunogen is immobilized on a solid phase and the antibody in the culture supernatant bound thereto is detected with an anti-mouse second antibody labeled with an enzyme.
- the monoclonal antibody is produced from the hybridoma by culturing the hybridoma in vitro or by culturing it in mouse, rat, guinea pig, hamster, rabbit or the like, preferably mouse or rat, more preferably mouse ascites, etc. It can be carried out by isolation from the supernatant or the ascites of mammals.
- the basic medium examples include a low calcium medium such as Ham'F12 medium, MCDB153 medium or low calcium MEM medium, and a high calcium medium such as MCDB104 medium, MEM medium, D-MEM medium, RPMI1640 medium, ASF104 medium or RD medium.
- the basic medium can contain, for example, serum, hormones, cytokines and / or various inorganic or organic substances depending on the purpose.
- affinity ammonium chromatography such as saturated ammonium sulfate, ion exchange chromatography (DEAE or DE52, etc.), anti-immunoglobulin column or protein A column, etc. Can be performed.
- an antibody gene is cloned from an antibody-producing cell such as a hybridoma, incorporated into an appropriate vector, introduced into a host, and a recombinant antibody produced using gene recombination technology.
- an antibody-producing cell such as a hybridoma
- an appropriate vector such as a hybridoma
- a recombinant antibody produced using gene recombination technology.
- variable region (V region) of an antibody is encoded from a hybridoma that produces the target antibody or an immune cell that produces the antibody, for example, a cell in which sensitized lymphocytes have been immortalized by an oncogene or the like.
- Isolate mRNA For isolation of mRNA, total RNA is prepared by a known method such as guanidine ultracentrifugation (Chirgwin, JM, et al., Biochemistry (1979) 18, 5294-5299), mRNA Purification Kit (manufactured by Pharmacia), etc. To prepare mRNA.
- Antibody V region cDNA is synthesized from the obtained mRNA using reverse transcriptase.
- the synthesis of cDNA can be performed using AMV Reverse Transscriptase First-strand cDNA Synthesis Kit or the like.
- 5'-Ampli FINDER RACEKit® manufactured by Clontech
- 5'-RACE method using PCR Frohman, MA, et al., Proc. Natl. Acad. Sci. USA
- the target DNA fragment is purified from the obtained PCR product and ligated with vector DNA.
- a recombinant vector is prepared from this, introduced into Escherichia coli, etc., and colonies are selected to prepare a desired recombinant vector.
- the base sequence of the target DNA is confirmed by a known method such as the deoxy method.
- DNA encoding the V region of the target antibody is obtained, it is ligated with DNA encoding the desired antibody constant region (C region) and incorporated into an expression vector.
- DNA encoding the V region of the antibody may be incorporated into an expression vector containing DNA of the antibody C region.
- the antibody gene is incorporated into an expression vector so as to be expressed under the control of an expression control region, for example, an enhancer / promoter.
- host cells can be transformed with this expression vector to express the antibody.
- the expression of the antibody gene may be carried out by co-transforming the host by separately incorporating the heavy chain (H chain) or light chain (L chain) of the antibody into an expression vector, or DNA encoding the H chain and L chain. May be incorporated into a single expression vector to transform the host (see WO94 / 11523).
- the monoclonal antibodies of the present invention include genetically modified monoclonal antibodies artificially modified for the purpose of reducing the heteroantigenicity to humans, such as chimeric monoclonal antibodies, humanized monoclonal antibodies, and human monoclonal antibodies. included.
- a chimeric monoclonal antibody consists of a V region derived from a non-human mammal antibody and a C region derived from a human antibody, and a humanized monoclonal antibody comprises a CDR derived from a non-human mammal antibody and FR and C regions derived from a human antibody. Since the antigenicity in the human body is reduced, it is useful as an antibody used in the present invention.
- These modified monoclonal antibodies can be produced using known methods.
- a chimeric monoclonal antibody is obtained by ligating the DNA encoding the antibody V region obtained as described above with DNA encoding the human antibody C region, incorporating it into an expression vector, introducing it into a host, and producing it (for example, WO95 / 14041). Using this known method, chimeric monoclonal antibodies useful in the present invention can be obtained.
- a humanized monoclonal antibody is obtained by transplanting a complementarity determining region (CDR) of a mammal other than a human, for example, a mouse antibody, into a CDR of a human antibody, and a general gene recombination technique is also known.
- CDR complementarity determining region
- oligonucleotides were prepared so that the DNA sequence designed to link the CDR of mouse antibody and the framework region (FR; framework region) of human antibody had an overlapping portion at the end. It is synthesized from nucleotides by PCR. The obtained DNA is obtained by ligating with DNA encoding the human antibody C region, then incorporating it into an expression vector, introducing it into a host and producing it (for example, WO95 / 14041).
- FR of a human antibody linked via CDR one in which CDR forms a favorable antigen binding site is selected. If necessary, the FR amino acids of the V region of the antibody may be substituted so that the CDRs of the reconstituted human antibody form an appropriate antigen-binding site (Sato, K. et al., Cancer Res. 53, 851).
- the human antibody C region is used for the chimeric monoclonal antibody and the humanized monoclonal antibody.
- Preferred human antibody C regions include C ⁇ , and for example, C ⁇ 1, C ⁇ 2, C ⁇ 3, C ⁇ 4 can be used.
- the human antibody C region may be modified to improve the stability of the antibody or its production.
- a human monoclonal antibody is composed of a V region and a C region derived from a human antibody, immunizes a human antibody-producing transgenic non-human mammal such as a human antibody-producing transgenic mouse (eg, WO94 / 25585) with an immunogen, and the monoclonal antibody Can be manufactured according to the existing general manufacturing method.
- the “part of the monoclonal antibody” is a part of the monoclonal antibody of the present invention described above, and means a region specifically binding to PcrV as in the case of the monoclonal antibody (hereinafter referred to as this).
- antibody fragments are also simply referred to as “antibody fragments”.
- Fab fragment of antigen binding
- F (ab ′) 2 single chain antibody
- Fab ′ single chain Fv
- scFv single chain Fv
- Disulfide stabilized antibodies disulfide stabilized Fv; hereinafter referred to as dsFv
- diabody dimerized V region fragments
- peptides containing CDRs etc.
- Fab is obtained by cleaving the upper peptide part of two disulfide bond (SS bond) ⁇ that crosses two H chains at the hinge region of IgG with the enzyme papain. It is an antibody fragment having an antigen-binding activity with a molecular weight of about 50,000, consisting of half and the entire L chain.
- the Fab used in the present invention can be obtained by treating the above monoclonal antibody of the present invention with papain.
- the Fab can also be produced by inserting a DNA encoding the Fab of the monoclonal antibody of the present invention into a cell expression vector and expressing the vector by introducing the vector into a cell.
- F (ab ′) 2 is composed of two Fab ′ regions obtained by decomposing the lower part of the two SS bonds in the hinge region of IgG with the enzyme pepsin and having a molecular weight of about It is an antibody fragment having an antigen binding activity of 100,000.
- F (ab ′) 2 used in the present invention can be obtained by treating the monoclonal antibody of the present invention with pepsin.
- F (ab ′) can also be expressed by inserting DNA encoding F (ab ′) 2 of the monoclonal antibody into an expression vector for cells and introducing the vector into Escherichia coli, yeast, or animal cells. 2 can be manufactured.
- Fab ′ is an antibody fragment having an antigen binding activity of about 50,000 molecular weight obtained by cleaving the SS bond between the hinges of F (ab ′) 2 .
- Fab ′ used in the present invention can be obtained by treating F (ab ′) 2 of the monoclonal antibody of the present invention with a reducing agent dithiothreitol.
- Fab ′ can also be produced by inserting a DNA encoding Fab ′ of the monoclonal antibody into an expression vector for cells and expressing the vector by introducing the vector into Escherichia coli, yeast, or animal cells.
- scFv is a VH-P-VL or VL-P-VH polypeptide in which one VH and one VL are linked using an appropriate peptide linker (hereinafter referred to as P) ⁇ , and has antigenic activity. It is an antibody fragment having The VH and VL contained in the scFv used in the present invention may be those of the above monoclonal antibody of the present invention.
- the scFv used in the present invention is expressed by obtaining cDNA encoding VH and VL from the hybridoma producing the monoclonal antibody of the present invention, constructing an scFv expression vector, and introducing it into Escherichia coli, yeast, or animal cells. Can be manufactured.
- DsFv refers to a polypeptide in which one amino acid residue in each of VH and VL is replaced with a cysteine residue, which are linked via an SS bond.
- the amino acid residue to be substituted with the cysteine residue can be selected based on the three-dimensional structure prediction of the antibody according to the method (Protein Engineering, 7, 697 (1994)) shown by Reiter et al.
- VH or VL contained in dsFv used in the present invention may be that of the monoclonal antibody of the present invention.
- dsFv used in the present invention
- cDNAs encoding VH and VL are obtained from the hybridoma producing the monoclonal antibody of the present invention and inserted into an appropriate expression vector to construct a dsFv expression vector.
- Yeast or animal cells can be introduced and expressed.
- Diabody is an antibody fragment in which scFv having the same or different antigen binding specificity forms a dimer, and has bivalent antigen binding activity against the same antigen or two types of specific antigen binding activities against different antigens. It is an antibody fragment.
- a divalent diabody that specifically reacts with the monoclonal antibody of the present invention obtains cDNA encoding the VH and VL of the monoclonal antibody of the present invention, and encodes scFv with a peptide linker of 3 to 10 residues. It can be produced by constructing DNA, inserting the DNA into an expression vector for cells, and introducing the expression vector into Escherichia coli, yeast, or animal cells to express diabody.
- the peptide containing CDR is configured to contain at least one region of CDR of VH or VL. Multiple CDRs can be linked directly or via a suitable peptide linker.
- the CDR-containing peptide used in the present invention is obtained by obtaining cDNA encoding the VH and VL of the monoclonal antibody of the present invention, constructing a DNA encoding the CDR, and inserting the DNA into an expression vector for animal cells.
- the vector can be produced by introducing the vector into Escherichia coli, yeast, or animal cells.
- a peptide containing CDR can also be produced by chemical synthesis methods such as Fmoc method (fluorenylmethyloxycarbonyl method) and tBoc method (t-butyloxycarbonyl method).
- the monoclonal antibody of the present invention or a part thereof may be a modified product as long as it can be suitably used in the present invention.
- the modified product an antibody bound to various molecules such as polyethylene glycol (PEG) can be used.
- PEG polyethylene glycol
- the modification made to the antibody may be a modification by introducing a chemical bond, or may be a modification made to the amino acid sequence of the antibody.
- These antibody modifications are also included in the monoclonal antibody of the present invention or a part thereof. In order to obtain such a modified antibody, it can be obtained by modifying the obtained antibody. These methods are already established in this field.
- the monoclonal antibody of the present invention or a part thereof is useful as a pharmaceutical composition. Therefore, the pharmaceutical composition containing the monoclonal antibody of the present invention and a part thereof can be systemically or locally administered orally or parenterally.
- parenteral administration for example, intravenous injection such as infusion, intramuscular injection, intraperitoneal injection, subcutaneous injection, intranasal administration, inhalation, etc. can be selected. It is known to damage epithelial cells and alveolar macrophages (T. Sawa et al., Nature Medicine, 1999, Vol. 5, page 392). Intranasal administration and inhalation are desirable.
- the pharmaceutical composition of the present invention is administered for the treatment of cystic fibrosis and infection patients caused by Pseudomonas aeruginosa.
- the effective dose is selected from the range of 0.01 mg to 100 mg per kg body weight at a time.
- a dose of 1-1000 mg, preferably 5-50 mg per patient can be selected.
- the pharmaceutical composition containing the monoclonal antibody of the present invention or a part thereof is not limited to these doses.
- the administration period can be appropriately selected depending on the age and symptoms of the patient.
- the pharmaceutical composition of the present invention may contain both pharmaceutically acceptable carriers and additives depending on the route of administration.
- Such carriers and additives include water, pharmaceutically acceptable organic solvents, collagen, polyvinyl alcohol, polyvinyl pyrrolidone, sodium alginate, water-soluble dextran, pectin, methyl cellulose, ethyl cellulose, casein, diglycerin, propylene glycol , Polyethylene glycol, petrolatum, human serum albumin (HSA), mannitol, sorbitol, lactose, surfactants acceptable as pharmaceutical additives, and the like.
- the additive to be used is selected appropriately or in combination from the above depending on the dosage form, but is not limited thereto. Reference example
- Mab166 (Japanese Patent Application No. 2005-500250, etc.) was produced as a recombinant antibody.
- mRNA was extracted from a hybridoma producing an antibody whose subclass is IgG2a, and the constant regions of the H chain and L chain were cloned by RT-PCR.
- Each fragment amplified by PCR was inserted into a pcDNA3.1 (+) vector (manufactured by Invitrogen) at the NheI and NotI sites, and a multiple cloning site was incorporated so that the DNA fragment of the variable region could be inserted.
- the H chain and L chain gene sequences of the Mab166 variable region were each divided into four, and then the sense DNA and antisense DNA were synthesized and annealed.
- the annealed fragments were ligated by DNA ligase, and the H chain was cloned into the MfeI and Blp I regions, and the L chain was cloned into the EcoRV and BsiW I regions.
- the H chain and L chain vectors whose base sequences were confirmed were introduced into HEK239T cells using Lipofectamine 2000 (manufactured by Invitrogen). After 48 hours, the cell supernatant was recovered.
- Recombinant Mab166 was purified from the collected cell supernatant using a Protein-G (PIERCE) column.
- PIERCE Protein-G
- the amplified PCR fragment was cloned into the pQE30 vector (GE Healthcare) at the Sph I and Hind III sites. After confirming the base sequence, this vector was introduced into E. coli JM109 to obtain recombinant E. coli (PcrV-JM109).
- PcrV-JM109 was cultured in 500 ml of LB / Ampicillin liquid medium at 37 ° C., and when OD600 reached 0.5, 200 ⁇ l of 0.1M IPTG was added to induce PcrV expression. Further, after culturing at 37 ° C.
- buffer A 25 mM Tris-HCl (pH 8.0), 0.5 M NaCl containing 0.5% lysozyme (manufactured by Sigma)
- 15 ml of 2 mM MgCl2 was added, and the mixture was allowed to stand at 0 ° C. for 30 minutes, and then sonicated.
- a soluble fraction was obtained, passed through His-Bind Columns (manufactured by Novagen), and then eluted with buffer B (200 mM phosphate buffer (pH 7.4), 500 mM NaCl) containing 200 mM imidazole.
- the final elution fraction was dialyzed against 10 mM phosphate buffer (pH 7.4) to perform buffer replacement.
- Biotin labeling of antigen As described above, PcrV protein expressed and purified was reacted in a final concentration 10 mM mercaptoethylamine solution at 37 ° C for 150 minutes to reduce cysteine residues. To the reduced SH group, 20 times the molar amount of PEO-Maleimide activated biotin (manufactured by PIERCE) was added and reacted at 4 ° C. overnight, and then dialyzed to remove excess biotin.
- Spleen cells and mouse myeloma cells were fused with 50% polyethylene glycol 4000 and selected in a medium containing hypoxanthine, aminopterin, and thymidine.
- the immunoassay used for the screening is as follows. 200 ⁇ l of Tris buffer solution (50 mM Tris-HCl, pH 7.5) containing 2 ⁇ g of anti-mouse IgG antibody (manufactured by Shiba Goat) was added to each well of a 96-well microtiter plate (manufactured by NUNK) and fixed at 4 ° C. for 16 hours. did.
- the plate was washed 3 times with 300 ⁇ l of washing solution, and the enhancement reagent (1.39 g / l potassium phthalate, 19.3 mg / l Tri-n-octylphosphine oxide, 4.59 mg / l 2-naphthoyltrifluoracetone, 1.0 g / l 200 ⁇ l of 1 Triton-X100, 6.0 g / l acetic acid) was added, and the time-resolved fluorescence was measured.
- the enhancement reagent (1.39 g / l potassium phthalate, 19.3 mg / l Tri-n-octylphosphine oxide, 4.59 mg / l 2-naphthoyltrifluoracetone, 1.0 g / l 200 ⁇ l of 1 Triton-X100, 6.0 g / l acetic acid
- 1F3 was IgG2a
- 2A4 was IgG2b
- 6F5 was IgG2a
- 7A7 was IgG2a.
- the hybridoma that produces monoclonal antibodies 1F3, 2A4, 6F5, and 7A7 was founded on October 18, 2007 in the National Institute of Advanced Industrial Science and Technology, Patent Biological Depositary Center (1-1-1 Higashi 1-1-1, Tsukuba, Ibaraki).
- the deposit numbers are FERM P-21403, FERM P-21404, FERM P-21405, and FERM ⁇ ⁇ ⁇ ⁇ P-21406, respectively.
- Antibody binding activity A competitive immunoassay was performed to measure the binding activity of the antibodies (1F3, 2A4, 6F5, 7A7).
- 100 ⁇ l of Tris buffer (50 mM Tris-HCl, pH 7.5) containing 1.5 ⁇ g of anti-mouse Fc antibody (Jackson ImmunoResearch) was added to each well of a 96-well microtiter plate (Nunk) at 4 ° C. Fixed for 16 hours. These wells were washed twice with 300 ⁇ l of washing solution, and then 300 ⁇ l of a block ace (Dainippon Pharmaceutical Co., Ltd.) solution containing 10% sucrose was added and left at room temperature for 2 hours for blocking (anti-mouse IgG antibody). Solid phase plate).
- the affinity of Mab166 is 3.0 ⁇ 10 ⁇ 9
- 1F3 is 3.7 ⁇ 10 ⁇ 10
- 2A4 is 3.5 ⁇ 10 ⁇ 10
- 6F5 is 1.1 ⁇ 10 9.
- ⁇ 10 7A7 was 1.1 ⁇ 10 ⁇ 9
- both clones had higher affinity than Mab166 (FIG. 2)
- Cytotoxicity inhibitory activity test 1F3, 2A4, 6F5, and 7A7 were subjected to a cytotoxicity inhibitory activity test. The method is as follows. First, 1F3, 2A4, 6F5, and 7A7 were diluted from 32 ⁇ g / ml in a 2-fold dilution series, and 10 ⁇ l was dispensed into each well of a 96-well microplate.
- the myeloma cell P3U1 (obtained from ATCC) was added to 5 ⁇ 10 6 Cells / ml or U937 (obtained from ATCC) cells, which are a type of white blood cell, to 1 ⁇ 10 6 Cells / ml, and the medium for cell culture (sodium bicarbonate And L-glutamine and phenol red-free RPMI 1640 (manufactured by Sigma)) and 100 ⁇ l of each was added to the 96-well microplate.
- the medium for cell culture sodium bicarbonate And L-glutamine and phenol red-free RPMI 1640 (manufactured by Sigma)
- the cytotoxic inhibitory activity (IC50) of Mab166 was 213 nM or higher, whereas 1F3 was 5.3 nM, 2A4 was 20.7 nM, 6F5 was 12.7 nM, 7A7 is 14.7 nM.
- IC50 cytotoxic inhibitory activity
- Mab166 is 54 nM, whereas 1F3 is 4.0 nM, 2A4 is 16 nM, 6F5 is 7.3 nM, and 7A7 is 6.0 nM. It was.
- 1F3, 2A4, 6F5, and 7A7 are compared with Mab166 (Frank et al., The Journal of Infectious Diseases, 2002, Vol. 186, page 66), which is known to have strong activity against any cell. Even so, it had strong cytotoxicity-inhibiting activity (FIGS. 4 and 5).
- deletion mutation PcrV (136-233) was prepared by the following method. PCR was performed using the PcrV antigen protein expression plasmid pQE30-PcrV as a template and using the 5 ′ primer GCTCGAGGATCCCAAGGCCGCTGACCGC (SEQ ID NO: 5) and 3 ′ primer GTTAAGCTTCTCGAAGGGGTACTC (SEQ ID NO: 6), and the amplified fragment was BamHI and HindIII After restriction enzyme treatment, it was inserted into pET32b (manufactured by Novagen).
- this vector was introduced into Escherichia coli BL21-DE3 strain to obtain recombinant Escherichia coli (deletion mutant PcrV-BL21).
- This expression strain was pre-cultured in 2 ml of LB / Ampicillin liquid medium at 37 ° C. overnight. 2 mL of preculture was added to 500 ml of LB / Ampillin liquid medium and cultured at 37 ° C. When the OD600 reached 0.5, the culture was allowed to stand on ice for 30 minutes. IPTG was added to a final concentration of 0.75 mM, and the cells were cultured overnight at 15 ° C. and 160 rpm in a shaking incubator to induce the expression of PcrV.
- the culture was collected by centrifugation at 4 ° C., x5000 g, 30 min. The supernatant was removed, and 10 ml of Buffer X (25 mM Tris-HCl (pH 7.5), 150 mM NaCl, 2 mM MgCl2) containing 0.1% lysozyme (manufactured by Sigma) was added to the cells and suspended. After leaving it to stand for 1 hour, it was subjected to ultrasonic crushing treatment while cooling with ice.
- Buffer X 25 mM Tris-HCl (pH 7.5), 150 mM NaCl, 2 mM MgCl2) containing 0.1% lysozyme (manufactured by Sigma) was added to the cells and suspended. After leaving it to stand for 1 hour, it was subjected to ultrasonic crushing treatment while cooling with ice.
- the possibility fraction was obtained by centrifugation, passed through a column packed with Ni-NTA agarose (Quiagen), and then eluted with Buffer Y (25 mM Tris-HCl (pH 7.5), 150 mM NaCl, 200 mM Imidazole). The final elution fraction was dialyzed against 10 mM phosphate buffer (pH 7.4) to perform buffer replacement.
- enhancement reagent (1.39 g / l potassium phthalate, 19.3 mg / l Tri-n-octylphosphine oxide, 4.59 mg / l 2-naphthoyltrifluoracetone, 1.0 g / l Triton- 200 ⁇ l of X100, 6.0 g / l acetic acid) was added, and the time-resolved fluorescence was measured (FIG. 6).
- the PcrV antibodies 1F3, 2A4, 9D12, 12H9, and m166 from KaloBios used as a reference example showed reactivity with the full length of PcrV (1-294).
- 1F3 and 2A4 showed reactivity against PcrV (136-233), but m166 and 9D12 and 12H9 without neutralizing activity showed no reactivity.
- a PcrV antibody solution diluted to 1 ⁇ g / ml was added to the membrane, reacted at 4 ° C. overnight, and then washed with washing solution B (10 mM phosphate buffer (pH 7.4), 0.05% Tween 20).
- washing solution B 10 mM phosphate buffer (pH 7.4), 0.05% Tween 20.
- a solution of anti-mouse IgG antibody (manufactured by GE Healthcare) labeled as a secondary antibody was added to the membrane, reacted for 2 hours at room temperature, the membrane was washed with washing solution B, and then ECL plus Western Blot Detection System (manufactured by GE Healthcare). Color was developed and photographed with LAS-1000 (FUJIFILM) (FIG. 7).
- PcrV neutralizing antibodies 1F3 and 2A4 reacted to both full-length PcrV and deletion mutant PcrV, while m166 and 6F5 and 7A7 with low neutralizing activity reacted only to full-length PcrV and to deletion mutant PcrV. Did not react.
- the epitope region of the PcrV neutralizing antibodies 1F3 and 2A4 was the region of amino acid residues 136-233, and m166, 6F5, and 7A7 did not have only the amino acid residues 136-233 as the epitope region.
- the test concentration ranges of 1F3, 2A4, and m166 in this test were 1.56-6.25 nM, 6.25-25 nM, and 50-200 nM, respectively.
- 10 ⁇ l of full length PcrV protein and deletion mutant PcrV protein were added to a 96-well plate at a molar concentration of 30, 10, 3, 1, 0.3 times for each test concentration range, and left at room temperature for 30 minutes.
- myeloma cells U3P1 were prepared in 5 ⁇ 10 6 cells / ml using a cell culture medium (containing sodium bicarbonate, L-glutamine and phenol red-free RPMI 1640 (manufactured by Sigma)), and the 96 wells. 70 ⁇ l of each was added to the microplate.
- the antibodies (1F3 and 2A4) having an epitope at amino acid residues 136-233 have stronger cytotoxic inhibitory activity than the antibody (m166) having no epitope in that region. That is, the strength of cytotoxicity-inhibiting activity differs depending on the region recognized by the PcrV antibody, and the strongest cytotoxicity-inhibiting activity can be said to be an antibody having reactivity with the PcrV protein only in the amino acid residue 136-233 region. It was.
- 1F3 was AGGGGCCCAGTGGATAGACCGATGGGCGCTGT (sequence number: 9)
- 2A4 was AGGGGCCCAGTGGATAGACTGATGGGGGGTGT (sequence number: 10).
- the amplified fragment was cloned using TOPO TA Cloning Kit (manufactured by Invitrogen), and the base sequence was analyzed using Applied Biosystems 3130 Genetic Analyzer (manufactured by Applied Biosystems).
- the analysis results of 1F3 are shown in FIG. 10, and 2A4 is shown in FIG.
- the monoclonal antibody of the present invention or a part thereof not only has a high affinity for PcrV, but also showed a strong inhibitory activity against the cytotoxic activity against eukaryotic cells of Pseudomonas aeruginosa (Pseudomonas aeruginosa). . Therefore, the pharmaceutical composition containing the monoclonal antibody or a part thereof is useful as a therapeutic agent for infectious diseases involving Pseudomonas aeruginosa, which is currently difficult to treat in the medical field.
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Medicinal Chemistry (AREA)
- Immunology (AREA)
- General Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Biophysics (AREA)
- General Chemical & Material Sciences (AREA)
- Pharmacology & Pharmacy (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Molecular Biology (AREA)
- Genetics & Genomics (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Oncology (AREA)
- Communicable Diseases (AREA)
- Engineering & Computer Science (AREA)
- Peptides Or Proteins (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
Description
(1)(A)インビトロにおいて1nMから200nMの濃度で、緑膿菌の白血球細胞に対する細胞傷害活性の50%以上を阻害する、
(B)インビトロにおいて1nMから50nMの濃度で、緑膿菌のミエローマ細胞に対する細胞傷害活性の50%以上を阻害する、および
(C)PcrVとの解離定数(Kd)が、2×10-9(M)以下である、
から選ばれる少なくとも1つの性質を有するPcrVに対するモノクローナル抗体またはその一部、
(2) 受託番号FERM P-21403として寄託されたハイブリドーマ、受託番号FERM P-21404として寄託されたハイブリドーマ、受託番号FERM P-21405として寄託されたハイブリドーマまたは受託番号FERM P-21406として寄託されたハイブリドーマのいずれかにより産生されるモノクローナル抗体の全ての相補性決定領域(CDR)のアミノ酸配列を有するモノクローナル抗体またはその一部、
(3)受託番号FERM P-21403として寄託されたハイブリドーマ、受託番号FERM P-21404として寄託されたハイブリドーマ、受託番号FERM P-21405として寄託されたハイブリドーマまたは受託番号FERM P-21406として寄託されたハイブリドーマのいずれかにより産生されるモノクローナル抗体またはその一部、
(4)配列番号1で示されるアミノ酸配列の136位から233位に、そのエピトープを有する、PcrVに対するモノクローナル抗体またはその一部、
(5)1)相補性決定領域に下記アミノ酸配列を含む重鎖可変領域;
SFTSYWMH(配列番号15)INPSNGRTNYNEKFNT(配列番号16)YGNYVVYYTMDY(配列番号17)および
2)相補性決定領域に下記アミノ酸配列を含む軽鎖可変領域;
SASTSVSYME(配列番号18)TTSKLAS(配列番号19)
HQWRNYPFT(配列番号20)
を有するPcrVに対するモノクローナル抗体またはその一部、
(6)1)相補性決定領域に下記アミノ酸配列を含む重鎖可変領域;
SITSDYAWN(配列番号21)YITYNGDTSYNPSLKS(配列番号22)SRNYYGAWFAY(配列番号23)および
2)相補性決定領域に下記アミノ酸配列を含む軽鎖可変領域;
KASQYVGTTVA(配列番号24)RASTRHT(配列番号25)
QQYCSSPLT(配列番号26)
を有するPcrVに対するモノクローナル抗体またはその一部、
(7)1)配列番号11のアミノ酸配列を有する重鎖可変領域;および
2)配列番号12のアミノ酸配列を有する軽鎖可変領域;を有するPcrVに対するモノクローナル抗体またはその一部、
(8)1)配列番号13のアミノ酸配列を有する重鎖可変領域;および
2)配列番号14のアミノ酸配列を有する軽鎖可変領域;を有するPcrVに対するモノクローナル抗体またはその一部、および
(9)上記(1)~(8)のいずれかに記載の抗体又はその一部を有効成分として含む医薬組成物、および
(10)上記(1)~(8)のいずれかに記載の抗体又はその一部を産生するハイブリドーマ、
に関する。
(2)インビトロにおいて1nMから50nMの濃度で、緑膿菌のミエローマ細胞に対する細胞傷害活性の50%以上を阻害する、および
(3)PcrVとの解離定数(Kd)が、2×10-9(M)以下である、
から選ばれる少なくとも1つの性質を有するPcrVに対するモノクローナル抗体である。
次に、緑膿菌の毒素などにより影響を受ける細胞(以下、ターゲット細胞とする)を細胞培養用の培地などを用いて希釈を行い、適当な数となるように調整する。具体的には、ミエローマ細胞を用いる場合であれば3×106~5×106Cells/ml、白血球細胞を用いるのであれば1×106~3×106Cells/mlの範囲で調整することが望ましい。同様にして、緑膿菌についても培養用の培地などで1×107~1×108cfu/mlとなるように調整する。そして、上述したモノクローナル抗体の存在下において、緑膿菌およびターゲット細胞を、同一の試験管やウェル(例えば、マイクロプレート上のウェルなどのインビトロの条件)において適度な培養条件により培養する。このときの培養条件は細胞やバクテリアを生育させるのに良好とされる一般的な培養条件でよい。また、培養時間はターゲット細胞の種類により最適な条件は適宜変更するが、例えばミエローマ細胞を用いる場合であれば1~3時間程度、白血球細胞を用いる場合であれば1~3時間程度が好ましい。抗体を添加しないウェルを対照群として、対照群に対して50%を阻害する濃度(有効濃度)を算出する。ターゲット細胞の生死の判定については種々の手法が確立されているが、呈色試薬を添加した後に、適当な波長(例えば、400~500nm)における吸光度の測定などが有用である(参考文献:Nature Medicine、1999年、vol.5、392~395ページ)。
そしてb)(i)CDR1はアミノ酸配列 SASTSVSYME(配列番号18)を有し、CDR2はアミノ酸配列TTSKLAS(配列番号19)を有し、CDR3はアミノ酸配列HQWRNYPFT(配列番号20)を有する、免疫グロブリン軽鎖可変領域(VL)および(ii)ヒト軽鎖の定常部分またはその断片を含んでいることが望ましい。
そしてb)(i)CDR1はアミノ酸配列KASQYVGTTVA(配列番号24)を有し、CDR2はアミノ酸配列RASTRHT(配列番号25)を有し、CDR3はアミノ酸配列QQYCSSPLT(配列番号26)を有する、免疫グロブリン軽鎖可変領域(VL)および(ii)ヒト軽鎖の定常部分またはその断片を含んでいることが望ましい。
(イ)PcrVを細胞表面に発現する細胞もしくは細胞表面に発現するように人工的に樹立した細胞株、またはPcrVを細胞表面に発現するように遺伝子組換え技術を用いて作成した遺伝子組換え細胞;
(ロ)PcrVまたはその一部をタンパク質として発現するように遺伝子組換え技術を用いて作成された遺伝子組換え細胞を培養して得た培養上清もしくは当該培養上清から精製されたPcrVまたはその一部;または
(ハ)化学的に合成されたPcrVまたはその一部。
このような担体および添加物の例として、水、医薬的に許容される有機溶媒、コラーゲン、ポリビニルアルコール、ポリビニルピロリドン、アルギン酸ナトリウム、水溶性デキストラン、ペクチン、メチルセルロース、エチルセルロース、カゼイン、ジグリセリン、プロピレングリコール、ポリエチレングリコール、ワセリン、ヒト血清アルブミン(HSA)、マンニトール、ソルビトール、ラクトース、医薬添加物として許容される界面活性剤などが挙げられる。使用される添加物は、剤型に応じて上記の中から適宜あるいは組合せて選択されるが、これらに限定されるものではない。
参考例
比較実験をするために、Mab166(特願2005-500250など)をリコンビナント抗体として作製した。
まずサブクラスがIgG2aである抗体を産生するハイブリドーマからmRNAを抽出し、H鎖およびL鎖の定常領域をRT-PCR法でクローニングした。PCRで増幅されたそれぞれの断片はpcDNA3.1(+)ベクター(インビトロジェン社製)にNheI、NotIサイトで挿入し、さらに可変領域部分のDNA断片を挿入できるようにマルチクローニングサイトを組み込んだ。
次にMab166可変領域部のH鎖およびL鎖の遺伝子配列をそれぞれ4分割した後、それらのセンスDNAとアンチセンスDNAを合成し、アニーリング反応した。アニーリング後の断片をDNAリガーゼによって結合させ、H鎖についてはMfeIとBlp I領域に、L鎖についてはEcoRVとBsiW I領域にクローニングを行った。
塩基配列が確認されたH鎖およびL鎖のベクターをLipofectamine2000(インビトロジェン社製)を用いて、HEK239T細胞へ導入し、48時間後、その細胞上清を回収した。回収された細胞上清からProtein-G(PIERCE社製)カラムでリコンビナントMab166を精製した。
以下、実施例を示して本発明を具体的に説明するが、本発明は下記実施例に制限されるものではない。なお、抗体作製手法として、特に断らない限り、Immunochemistry in Practice (Blackwell Scientific Publiations)に記載されている方法を用いた。また、遺伝子操作的手法として、特に断らない限り、Molecular Cloning:A Laboratory Manual,2nd Edition(Cold Spring Harbor Laboratory)に記載されている方法を用いた。
東海大より分与された緑膿菌株標準株PAO1の染色体DNAを抽出し、そのDNAを鋳型としてPCR法により、PcrVタンパク質をコードする遺伝子(配列番号:2)を増幅した。5’側プライマーに制限酵素Sph I認識部位を、3’側プライマーに制限酵素Hind III認識部位を設け(配列番号:3、4)、さらに発現ベクター挿入時に、ビオチン標識のためにヒスチジンタグと開始コドンの間にシステインが挿入されるように設計した。増幅されたPCR断片はSph IおよびHind III部位でpQE30ベクター(GEヘルスケア社製)へクローニングを行なった。塩基配列を確認後、大腸菌JM109へ本ベクターを導入し、組み換え大腸菌(PcrV-JM109)を得た。PcrV-JM109を500mlのLB/Ampicillin液体培地中に37℃で培養し、OD600が0.5になった時に0.1MのIPTGを200μl加え、PcrVの発現を誘導した。さらに、37℃で1.5時間培養後、菌体を遠心分離し、0.5%のリゾチーム(シグマ社製)を含む緩衝液A(25mM Tris-HCl(pH8.0)、0.5M NaCl、2mM MgCl2)を15 ml加え、0℃で30分間放置後、超音波処理を行った。遠心後、可溶性画分を得、His-Bind Columns(Novagen社製)に通した後、200mM イミダゾールを含む緩衝液B(20mM リン酸緩衝液(pH7.4)、500mM NaCl)で溶出した。最終的な溶出画分は10mMリン酸緩衝液(pH7.4)に対して透析することにより緩衝液置換を行った。
上記のように、発現・精製させたPcrVタンパク質を終濃度10mM メルカプトエチルアミン溶液中で37℃、150分反応させてシステイン残基を還元した。還元されたSH基にモル比で20倍量のPEO-Maleimide activated biotin(PIERCE社製)を添加し、4℃で終夜反応後、過剰なビオチンを除去するため、透析をおこなった。
抗原として精製したPcrV 20μgをフロイント完全アジュバントと共に4週齢Balb/c雌マウス7匹に腹腔内投与し、初回免疫とした。その後、14日後、35日後にPcrV 20μgをフロイント不完全アジュバントと共に投与し、追加免疫とした。さらに77日後にPcrV 20μgの腹腔投与とPcrV 10μgを尾静脈投与することで、最終免疫とした。
最終免疫の3日後に脾臓を摘出し、脾臓細胞を回収した。脾臓細胞とマウスミエローマ細胞(p3×63-Ag8.U1、東京腫瘤研究所)を50%のポリエチレングリコール4000を用いて融合させ、ヒポキサンチン、アミノプテリン、及びチミジンを含む培地で選択した。
細胞融合8日後に特異抗体産生細胞のスクリーニングを行った。スクリーニングに用いたイムノアッセイは以下の通りである。96穴マイクロタイタープレート(ヌンク社製)の各ウェルに2μgの抗マウスIgG抗体(シバヤギ社製)を含むトリス緩衝液(50mM Tris-HCl、pH7.5)を200μl加えて4℃で16時間固定した。これらのウェルを300μlの洗浄液(0.1% Tween20を含む生理食塩水)で2回洗浄した後、ブロックエース(大日本製薬社製)を300μl加えて室温で2時間放置して、ブロッキングを行った(抗マウスIgG抗体固相化プレート)。各ウェルを300μlの洗浄液で2回洗浄した後、50μlのハイブリドーマ培養上清を150μlの緩衝液C(0.9% 塩化ナトリウム、0.05% アジ化ナトリウム、0.5% ウシ血清アルブミン、0.01% Tween80、25μM Diethylenetriamine-N,N,N’,N‘’、N’ ’-pentaacetic acidを含む50mMトリス緩衝液、pH7.6)で希釈して各ウェルに添加し、4℃で終夜反応させた。300μlの洗浄液で3回洗浄後、10ngのEu-Labelled Streptavidin(PERKIN ELMER社製)と25ng ビオチン標識化PcrVを含む200μlの緩衝液Cを加え、室温1時間反応させた。反応後、300μlの洗浄液で3回洗浄を行い、増強試薬(1.39g/l フタル酸カリウム、19.3mg/l Tri-n-octylphosphine oxide、4.59mg/l 2-naphthoyltrifluoroacetone、1.0g/l Triton-X100、6.0g/l 酢酸)を200μl添加し、その時間分解蛍光を測定した。
抗体(1F3、2A4、6F5、7A7)の結合活性を測定するために、競合イムノアッセイを行った。96穴マイクロタイタープレート(ヌンク社製)の各ウェルに1.5μgの抗マウスFc抗体(Jackson ImmunoReseach社製)を含むトリス緩衝液(50mM Tris-HCl、pH7.5)を100μl加えて4℃で16時間固定した。これらのウェルを300μlの洗浄液で2回洗浄した後、10%スクロースを含むブロックエース(大日本製薬社製)溶液を300μl加えて室温で2時間放置して、ブロッキングを行った(抗マウスIgG抗体固相化プレート)。各ウェルを300μlの洗浄液で2回洗浄した後、2ng/ウェルの各抗体および未標識のPcrVを500ng/ウェルから10倍の希釈系列で5段階添加した。その後、5ng/ウェルのビオチン化PcrVを添加し終夜反応させた。300μlの洗浄液で4回洗浄し、100/ウェルのEnhancement Solution(Wallac社製)を添加後、1分間の攪拌後、時間分解蛍光を測定した。その結果、Mab166と比較して、1F3、2A4、6F5、7A7はPcrVとの強い結合活性を示した(図1)。
1F3、2A4、6F5、7A7はMab166とエピトープが異なることを証明するため、当該抗体とMab166とのサンドイッチ・アッセイの可否を検討した。
最初に、Mab166のビオチン標識を行った。100μgのMab166と7.853μgのNHS-PEO4 Biotin(PIERCE社製)を0.1M PBS(pH7.4)中で混合し、氷中で2時間反応させた。その後、反応溶液から未反応のビオチンを除去するため、ゲルクロマトグラフィー(G2000SWカラム(TOSHO社製))を行った。
1F3、2A4、6F5、7A7について、細胞傷害阻害活性試験を行った。その方法は以下の通りである。
まず1F3、2A4、6F5、7A7を、32μg/mlから2倍希釈系列で希釈し、96ウェルマイクロプレートの各ウェルに10μlずつ分注した。次にミエローマ細胞P3U1(ATCCから入手)を5×106Cells/mlもしくは白血球細胞の一種であるU937(ATCCから入手)細胞を1×106Cells/mlに細胞培養用培地(炭酸水素ナトリウムを含み、L-グルタミン及びフェノール・レッド不含RPMI1640(Sigma社製))を用いて調整し、当該96ウェルマイクロプレートに各100μl添加した。さらにCation-adjusted Mueller Hinton Broth(Difco)で一晩培養したシュードモナス・アエルギノーザSR24株を細胞培養用培地を用いて1×108cfu/mlに調整した菌液を10μl/ウェルで添加し、37℃、5%C02存在下で3時間培養した。3時間経過後、WST-8(キシダ化学社製)を各10μl添加し、ミエローマ細胞P3U1を用いた場合は、37℃、5%C02存在下で3時間、U937細胞の場合は1時間培養した。培養終了後、450nmの波長で吸光度を測定した。
欠失変異PcrV(136-233)は以下の方法で作製した。
PcrV抗原タンパク発現プラスミドであるpQE30―PcrVを鋳型とし、5’側プライマーGCTCGAGGATCCCAAGGCGCTGACCGC(配列番号5)、3’側プライマーGTTAAGCTTCTCGAAGGGGTACTC(配列番号6)を用いてPCRを行い、増幅したフラグメントをBamHI、HindIIIで制限酵素処理し、pET32b(Novagen社製)に挿入した。塩基配列を確認後、本ベクターを大腸菌BL21-DE3株に導入し組み換え大腸菌(欠失変異PcrV-BL21)を得た。この発現株を2mlのLB/Ampicillin液体培地で37℃、一昼夜前培養した。500mlのLB/Ampicillin液体培地中に2mLの前培養液を加えて37℃で培養し、OD600が0.5になった時に培養液を氷上で30分間静置した。終濃度0.75mMとなるようにIPTGを加え、震盪培養機で15℃、160rpm、一昼夜培養してPcrVの発現を誘導した。培養液を4℃、x5000g、30minで遠心して集菌した。上清を除き、菌体に0.1%のリゾチーム(シグマ社製)を含むBuffer X(25mM Tris-HCl(pH7.5)、150mM NaCl、2mM MgCl2)を10 ml加えて懸濁し、氷上で1時間静置した後、氷冷しながら超音波破砕処理した。遠心により可能性画分を得、Ni-NTAアガロース(Quiagen)を充填したカラムに通した後、Buffer Y(25mM Tris-HCl(pH7.5)、150mM NaCl、200mM Imidazole)で溶出した。最終的な溶出画分は10mMリン酸緩衝液(pH7.4)に対して透析することにより緩衝液置換を行った。
96穴マイクロタイタープレート(ヌンク社製)の各ウェルに1.5μgの抗マウスIgG Fc抗体(Jackson ImmunoResearch社製)を含むトリス緩衝液(50mM Tris-HCl、pH7.5)を150μl加えて4℃で16時間固定した。これらのウェルを300μlの洗浄液(0.1% Tween20を含む生理食塩水)で2回洗浄した後、ブロッキング溶液(50mM Tris-HCl pH7.5、2%ブロックエース(大日本製薬社製)、10%スクロース)を300μl加えて室温で2時間放置して、各ウェルをブロッキングした(抗マウスIgG抗体固相化プレート)。各ウェルを300μlの洗浄液で1回洗浄した後、緩衝液C(0.9% 塩化ナトリウム、0.05% アジ化ナトリウム、0.5% ウシ血清アルブミン、0.01% Tween80、25μM Diethylenetriamine-N,N,N’ ,N’ ’, N’ ’-pentaacetic acidを含む50mMトリス緩衝液、pH7.6)で80ng/mlに希釈したPcrV抗体溶液を50μlずつ各ウェルに添加し、Buffer Cで200ng/mlに希釈したEu-Labelled Streptavidin(PERKIN ELMER社製)溶液を各ウェルに50μlずつ、DELFIA Asssay Bufferで各濃度に希釈した欠損変異PcrVタンパクを各ウェルに100μlずつ加え4℃で終夜反応させた。300μlの洗浄液で3回洗浄後、200μlの増強試薬(1.39g/l フタル酸カリウム、19.3mg/l Tri-n-octylphosphine oxide、4.59mg/l 2-naphthoyltrifluoroacetone、1.0g/l Triton-X100、6.0g/l 酢酸)を200μl添加し、その時間分解蛍光を測定した(図6)。
またウエスタンブロット法による結合解析も行なった。精製したリコンビナントPcrVタンパクをSDS-PAGEを行った後、PVDFメンブレンに転写した。転写されたメンブレンはブロックエース(大日本製薬製)溶液で室温、2時間、振盪しながらブロッキングした。1μg/mlに希釈したPcrV抗体溶液をメンブレンに加え、4℃で終夜反応させた後、洗浄液B(10mM リン酸緩衝液(pH7.4)、0.05% Tween20)で洗浄した。2次抗体として標識した抗マウスIgG抗体(GE Healthcare社製)溶液をメンブレンに加え、室温で2時間反応後、メンブレンを洗浄液Bで洗浄し、ECL plus Western Blot Detection System(GE Healthcare社製)で発色させ、LAS-1000(FUJIFILM)で撮影した(図7)。PcrV中和抗体1F3、2A4は全長PcrVおよび欠失変異PcrVの双方に対して反応したが、m166、及び中和活性の低い6F5、7A7は全長PcrVに対してのみ反応し、欠失変異PcrVには反応しなかった。
全長PcrVタンパク質(配列番号1)および欠損変異PcrVタンパク質(配列番号1における136位-233位のアミノ酸配列を有する)を用いて,1F3、2A4、m166における細胞傷害阻害活性の抑制試験を行なった。その方法は以下の通りである。
まず1F3、2A4、m166をそれぞれ200nM、200nM、400nMから2倍希釈系列で希釈し、96ウェルマイクロプレートに10μlずつ分注した。本試験における1F3、2A4、m166の試験濃度域はそれぞれ1.56-6.25nM、6.25-25nM、50-200nMとした.各試験濃度域に対して全長PcrVタンパク質および欠損変異PcrVタンパク質を30、10、3、1、0.3倍モル濃度で96ウェルプレートに10μl添加し、30分室温で放置した.次にミエローマ細胞U3P1を5×106cells/mlに細胞培養用培地(炭酸水素ナトリウムを含み、L-グルタミン及びフェノール・レッド不含RPMI1640(Sigma社製))を用いて調製し、当該96ウェルマイクロプレートに各70μl添加した。さらにMueller Hinton Broth(Difco)で一晩培養した緑膿菌SR24株を細胞培養用培地を用いて1×108cfu/mlに調製した菌液を10μl添加し、37℃、5%C02存在下で3時間培養した。3時間経過後、WST-8(キシダ化学社製)を各10μl添加し、37℃、5%C02存在下で3時間培養した。培養終了後、450nmの波長で吸光度を測定した。
樹立されたハイブリドーマ細胞から、RNeasy Mini Kit(QIAGEN社製)を用いて、RNAの抽出を行った。抽出したRNAを1μgから、5’RACE Syatem for Rapid Amplification of cDNA Ends,Version 2.0(Invitrogen社製)を用いて、DNA断片の増幅を行った。その際、cDNA合成のために使用したプライマーは、1F3がTAGAGTCACCGAGGAGCCAGTTGT(配列番号:7)であり、2A4はTCCAGAGTTCCAAGTCACAGTCAC(配列番号:8)であった。また5’RACE法に用いたプライマーは、1F3がAGGGGCCAGTGGATAGACCGATGGGGCTGT(配列番号:9)であり、2A4がAGGGGCCAGTGGATAGACTGATGGGGGTGT(配列番号:10)であった。増幅された断片は、TOPO TA Cloning Kit(Invitrogen社製)でクローニングされ、Applied Biosystems 3130 Genetic Analyzer(Applied Biosystems社製)で塩基配列を解析した。1F3の解析結果を、図10に、2A4については図11に示した。
Claims (10)
- 以下の(1)~(3)から選ばれる少なくとも1つの性質を有するPcrVに対するモノクローナル抗体またはその一部:
(1)インビトロにおいて1nMから200nMの濃度で、緑膿菌の白血球細胞に対する細胞傷害活性の50%以上を阻害する、
(2)インビトロにおいて1nMから50nMの濃度で、緑膿菌のミエローマ細胞に対する細胞傷害活性の50%以上を阻害する、及び
(3)PcrVとの解離定数(Kd)が、2×10-9(M)以下である。 - 受託番号FERM P-21403として寄託されたハイブリドーマ、受託番号FERM P-21404として寄託されたハイブリドーマ、受託番号FERM P-21405として寄託されたハイブリドーマまたは受託番号FERM P-21406として寄託されたハイブリドーマのいずれかにより産生されるモノクローナル抗体の全ての相補性決定領域(CDR)のアミノ酸配列を有するモノクローナル抗体またはその一部。
- 受託番号FERM P-21403として寄託されたハイブリドーマ、受託番号FERM P-21404として寄託されたハイブリドーマ、受託番号FERM P-21405として寄託されたハイブリドーマまたは受託番号FERM P-21406として寄託されたハイブリドーマのいずれかにより産生されるモノクローナル抗体またはその一部。
- 配列番号1で示されるアミノ酸配列の136位から233位に、そのエピトープを有する、PcrVに対するモノクローナル抗体またはその一部。
- 1)相補性決定領域に下記アミノ酸配列を含む重鎖可変領域; SFTSYWMH(配列番号15)INPSNGRTNYNEKFNT(配列番号16)YGNYVVYYTMDY(配列番号17)および 2)相補性決定領域に下記アミノ酸配列を含む軽鎖可変領域;SASTSVSYME(配列番号18)TTSKLAS(配列番号19) HQWRNYPFT(配列番号20)を有するPcrVに対するモノクローナル抗体またはその一部。
- 1)相補性決定領域に下記アミノ酸配列を含む重鎖可変領域; SITSDYAWN(配列番号21)YITYNGDTSYNPSLKS(配列番号22)SRNYYGAWFAY(配列番号23)および2)相補性決定領域に下記アミノ酸配列を含む軽鎖可変領域;KASQYVGTTVA(配列番号24)RASTRHT(配列番号25)QQYCSSPLT(配列番号26)を有するPcrVに対するモノクローナル抗体またはその一部。
- 1)配列番号11のアミノ酸配列を有する重鎖可変領域;および 2)配列番号12のアミノ酸配列を有する軽鎖可変領域;を有するPcrVに対するモノクローナル抗体またはその一部。
- 1)配列番号13のアミノ酸配列を有する重鎖可変領域;および 2)配列番号14のアミノ酸配列を有する軽鎖可変領域;を有するPcrVに対するモノクローナル抗体またはその一部。
- 請求項1~8のいずれかに記載の抗体又はその一部を有効成分として含む医薬組成物。
- 請求項1~8のいずれかに記載の抗体又はその一部を産生するハイブリドーマ。
Priority Applications (11)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN200980109395.6A CN102137870B (zh) | 2008-01-10 | 2009-01-08 | 抗PcrV抗体 |
CA2711863A CA2711863C (en) | 2008-01-10 | 2009-01-08 | Antibody directed against pcrv |
ES09701235T ES2426093T3 (es) | 2008-01-10 | 2009-01-08 | Anticuerpo dirigido contra PcrV |
EA201070826A EA020544B1 (ru) | 2008-01-10 | 2009-01-08 | АНТИТЕЛА ПРОТИВ PcrV |
BRPI0906810A BRPI0906810A2 (pt) | 2008-01-10 | 2009-01-08 | anticorpo contra pcrv |
KR1020107017717A KR101259239B1 (ko) | 2008-01-10 | 2009-01-08 | PcrV에 대한 항체 |
EP09701235.5A EP2248826B1 (en) | 2008-01-10 | 2009-01-08 | Antibody directed against pcrv |
PL09701235T PL2248826T3 (pl) | 2008-01-10 | 2009-01-08 | Przeciwciało skierowane przeciwko PcrV |
US12/863,983 US8501179B2 (en) | 2008-01-10 | 2009-01-08 | Antibody against PcrV |
AU2009203426A AU2009203426C1 (en) | 2008-01-10 | 2009-01-08 | Antibody directed against PcrV |
JP2009548942A JP4766716B2 (ja) | 2008-01-10 | 2009-01-08 | PcrVに対する抗体 |
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP2008-003214 | 2008-01-10 | ||
JP2008003214 | 2008-01-10 |
Publications (1)
Publication Number | Publication Date |
---|---|
WO2009088032A1 true WO2009088032A1 (ja) | 2009-07-16 |
Family
ID=40853144
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/JP2009/050118 WO2009088032A1 (ja) | 2008-01-10 | 2009-01-08 | PcrVに対する抗体 |
Country Status (13)
Country | Link |
---|---|
US (1) | US8501179B2 (ja) |
EP (2) | EP2599792A1 (ja) |
JP (1) | JP4766716B2 (ja) |
KR (1) | KR101259239B1 (ja) |
CN (1) | CN102137870B (ja) |
AU (1) | AU2009203426C1 (ja) |
BR (1) | BRPI0906810A2 (ja) |
CA (1) | CA2711863C (ja) |
EA (1) | EA020544B1 (ja) |
ES (1) | ES2426093T3 (ja) |
PL (1) | PL2248826T3 (ja) |
PT (1) | PT2248826E (ja) |
WO (1) | WO2009088032A1 (ja) |
Cited By (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2010104052A1 (ja) * | 2009-03-11 | 2010-09-16 | 塩野義製薬株式会社 | 抗緑膿菌作用を有するヒト化PcrV抗体 |
US8501179B2 (en) | 2008-01-10 | 2013-08-06 | Shionogi & Co., Ltd. | Antibody against PcrV |
JP2015509723A (ja) * | 2012-03-02 | 2015-04-02 | アブリンクス エン.ヴェー. | シュードモナス・エルギノーサのPcrVに結合する単一可変ドメイン抗体 |
WO2020252029A1 (en) | 2019-06-11 | 2020-12-17 | Regeneron Pharmaceuticals, Inc. | Anti-pcrv antibodies that bind pcrv, compositions comprising anti-pcrv antibodies, and methods of use thereof |
Families Citing this family (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
BR112013031485B1 (pt) | 2011-06-10 | 2022-06-14 | Medimmune, Llc | Anticorpo monoclonal isolado ou fragmento de ligação a antígeno do mesmo, composição farmacêutica, usos e método in vitro para bloqueio ou prevenção da ligação de p. aeruginosa a células epiteliais |
CA3161431A1 (en) * | 2011-11-07 | 2013-05-16 | Medimmune Limited | Combination therapies using anti-pseudomonas psl and pcrv binding molecules |
WO2021244421A1 (en) * | 2020-06-01 | 2021-12-09 | Staidson (Beijing) Biopharmaceuticals Co., Ltd. | Antibodies specifically recognizing pseudomonas pcrv and uses thereof |
CN118440191A (zh) * | 2024-05-11 | 2024-08-06 | 重庆原伦生物科技有限公司 | 一种抗铜绿假单胞菌PcrV抗体的制备及其应用 |
Citations (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO1994011523A2 (en) | 1992-11-13 | 1994-05-26 | Idec Pharmaceuticals Corporation | Fully impaired consensus kozac sequences for mammalian expression |
WO1994025585A1 (en) | 1993-04-26 | 1994-11-10 | Genpharm International, Inc. | Transgenic non-human animals capable of producing heterologous antibodies |
WO1995014041A1 (fr) | 1993-11-19 | 1995-05-26 | Chugai Seiyaku Kabushiki Kaisha | Anticorps humain reconstitue contre des cellules medulloblastomateuses humaines |
US6551795B1 (en) | 1998-02-18 | 2003-04-22 | Genome Therapeutics Corporation | Nucleic acid and amino acid sequences relating to pseudomonas aeruginosa for diagnostics and therapeutics |
JP2005500250A (ja) | 2001-01-26 | 2005-01-06 | エムシーダブリユー リサーチ フオンデーシヨン インコーポレーテツド | シュードモナスv抗原を用いる免疫化のための方法および組成物 |
WO2007114340A1 (ja) * | 2006-03-30 | 2007-10-11 | Meiji Seika Kaisha, Ltd. | 緑膿菌の外膜タンパク質pa0427 |
WO2009005040A1 (ja) * | 2007-06-29 | 2009-01-08 | Meiji Seika Kaisha Ltd. | 緑膿菌の外膜タンパク質pa4710 |
Family Cites Families (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE69930166T2 (de) * | 1998-11-25 | 2006-12-14 | MCW Research Foundation, Inc., Milwaukee | Verfahren und zusammensetzungen für impfung mit dem pseudomonas aeruginosa v-antigen |
US20030228324A1 (en) * | 1999-05-06 | 2003-12-11 | Malcolm Andrew J. | Peptide compositions and methods of producing and using same |
US7981843B2 (en) * | 2004-01-20 | 2011-07-19 | Kalobios Pharmaceuticals, Inc. | Antibody specificity transfer using minimal essential binding determinants |
CN101002947B (zh) * | 2007-01-16 | 2010-08-25 | 湖南农业大学 | 一种用于细菌病治疗的靶向药物及其制备方法 |
BRPI0819598A2 (pt) | 2007-11-30 | 2017-05-09 | Kalobios Pharmaceuticals Inc | anticorpos para o antígeno pcrv da pseudomonas aeruginosa |
ES2426093T3 (es) | 2008-01-10 | 2013-10-21 | Shionogi & Co., Ltd. | Anticuerpo dirigido contra PcrV |
PT2407537E (pt) | 2009-03-11 | 2015-02-17 | Shionogi & Co | Anticorpo pcrv humanizado com atividade anti-pseudomonal |
-
2009
- 2009-01-08 ES ES09701235T patent/ES2426093T3/es active Active
- 2009-01-08 EP EP13152512.3A patent/EP2599792A1/en not_active Withdrawn
- 2009-01-08 AU AU2009203426A patent/AU2009203426C1/en not_active Ceased
- 2009-01-08 CA CA2711863A patent/CA2711863C/en not_active Expired - Fee Related
- 2009-01-08 EP EP09701235.5A patent/EP2248826B1/en active Active
- 2009-01-08 BR BRPI0906810A patent/BRPI0906810A2/pt not_active IP Right Cessation
- 2009-01-08 PT PT97012355T patent/PT2248826E/pt unknown
- 2009-01-08 CN CN200980109395.6A patent/CN102137870B/zh active Active
- 2009-01-08 WO PCT/JP2009/050118 patent/WO2009088032A1/ja active Application Filing
- 2009-01-08 PL PL09701235T patent/PL2248826T3/pl unknown
- 2009-01-08 KR KR1020107017717A patent/KR101259239B1/ko not_active IP Right Cessation
- 2009-01-08 EA EA201070826A patent/EA020544B1/ru not_active IP Right Cessation
- 2009-01-08 JP JP2009548942A patent/JP4766716B2/ja active Active
- 2009-01-08 US US12/863,983 patent/US8501179B2/en active Active
Patent Citations (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO1994011523A2 (en) | 1992-11-13 | 1994-05-26 | Idec Pharmaceuticals Corporation | Fully impaired consensus kozac sequences for mammalian expression |
WO1994025585A1 (en) | 1993-04-26 | 1994-11-10 | Genpharm International, Inc. | Transgenic non-human animals capable of producing heterologous antibodies |
WO1995014041A1 (fr) | 1993-11-19 | 1995-05-26 | Chugai Seiyaku Kabushiki Kaisha | Anticorps humain reconstitue contre des cellules medulloblastomateuses humaines |
US6551795B1 (en) | 1998-02-18 | 2003-04-22 | Genome Therapeutics Corporation | Nucleic acid and amino acid sequences relating to pseudomonas aeruginosa for diagnostics and therapeutics |
JP2005500250A (ja) | 2001-01-26 | 2005-01-06 | エムシーダブリユー リサーチ フオンデーシヨン インコーポレーテツド | シュードモナスv抗原を用いる免疫化のための方法および組成物 |
WO2007114340A1 (ja) * | 2006-03-30 | 2007-10-11 | Meiji Seika Kaisha, Ltd. | 緑膿菌の外膜タンパク質pa0427 |
WO2009005040A1 (ja) * | 2007-06-29 | 2009-01-08 | Meiji Seika Kaisha Ltd. | 緑膿菌の外膜タンパク質pa4710 |
Non-Patent Citations (23)
Title |
---|
CARL ET AL., THERAPEUTIC MONOCLONAL ANTIBODIES, 1990 |
CHIRGWIN, J. M. ET AL., BIOCHEMISTRY, vol. 18, 1979, pages 5294 - 5299 |
DARA W. FRANK ET AL., J. INFECT. DISEASE, vol. 186, 2002, pages 64 |
DATABASE NCBI 19 November 1997 (1997-11-19), YAHR T.L. ET AL.: "Pseudomonas aeruginosa PcrG (pcrG), PcrV (pcrV), PcrH (pcrH), PopB (popB) and PopD (popD) genes, complete cds.", retrieved from http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi? 2459966:NCBI:664056 Database accession no. AF010149 * |
EXPERT OPINION ON THERAPEUTIC PATENTS, vol. 6, no. 5, 1996, pages 441 - 456 |
FAURE K. ET AL.: "Effects of monoclonal anti-PcrV antibody on Pseudomonas aeruginosa-induced acute lung injury in a rat model.", J. IMMUNE BASED THER. VACCINES, vol. 1, no. 2, 2003, pages 1 - 9 * |
FRANK ET AL., THE JOURNAL OF INFECTIOUS DISEASES, vol. 186, 2002, pages 66 |
FROHMAN, M. A. ET AL., PROC. NATL. ACAD. SCI. USA, vol. 85, 1988, pages 8998 |
HORIBATA, K.; HARRIS, A. W., NATURE, vol. 256, 1975, pages 495 - 497 |
IMAMURA Y ET AL., EUR. RESPIR. J., vol. 29, 2007, pages 965 |
KARINE FAURE ET AL., J. IMMUNE. BASED. THERAPIES AND VACCINES, vol. 1, 2003 |
KEARNEY, J. F. ET AL., J. IMMUNOLOGY, vol. 123, 1979, pages 1548 - 1550 |
KOHLER, G ET AL., EUROPEAN J. IMMUNOLOGY, vol. 6, 1976, pages 511 - 519 |
NATURE MEDICINE, vol. 5, 1999, pages 392 - 395 |
NATURE, vol. 256, 1975, pages 495 - 497 |
SATO, K. ET AL., CANCER RES., vol. 53, 1993, pages 851 |
See also references of EP2248826A4 * |
SHIME N ET AL., J. IMMUNOL., vol. 167, 2001, pages 5880 |
SHULMAN, M. ET AL., NATURE, vol. 276, 1978, pages 269 - 270 |
T. SAWA ET AL., NATURE MEDICINE, vol. 5, 1999, pages 392 |
YAHR T.L. ET AL.: "Identification of type III secreted products of the Pseudomonas aeruginosa exoenzyme S regulon.", J. BACTERIOL., vol. 179, 1997, pages 7165 - 7168 * |
YAHR, T.L. ET AL., J. BACTERIOL., vol. 179, 1997, pages 7165 |
YELTON, D.E. ET AL., CURRENT TOPICS IN MICROBIOLOGY AND IMMUNOLOGY, vol. 81, 1978, pages 1 - 7 |
Cited By (13)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US8501179B2 (en) | 2008-01-10 | 2013-08-06 | Shionogi & Co., Ltd. | Antibody against PcrV |
US9085611B2 (en) | 2009-03-11 | 2015-07-21 | Shionogi & Co., Ltd. | Humanized PcrV antibody having anti-pseudomonal activity |
EP2407537A4 (en) * | 2009-03-11 | 2012-12-26 | Shionogi & Co | HUMANIZED ANTI-PCRV ANTIBODIES WITH ANTI-PSEUDOMONAS ACTIVITY |
EP2407537A1 (en) * | 2009-03-11 | 2012-01-18 | Shionogi & Co., Ltd. | Humanized pcrv antibody having anti-pseudomonal activity |
EA021101B1 (ru) * | 2009-03-11 | 2015-04-30 | Шионоги Энд Ко., Лтд. | ГУМАНИЗИРОВАННОЕ АНТИТЕЛО К PcrV, ОБЛАДАЮЩЕЕ АКТИВНОСТЬЮ ПРОТИВ ПСЕВДОМОНАС |
WO2010104052A1 (ja) * | 2009-03-11 | 2010-09-16 | 塩野義製薬株式会社 | 抗緑膿菌作用を有するヒト化PcrV抗体 |
JP2015509723A (ja) * | 2012-03-02 | 2015-04-02 | アブリンクス エン.ヴェー. | シュードモナス・エルギノーサのPcrVに結合する単一可変ドメイン抗体 |
US10072098B2 (en) | 2012-03-02 | 2018-09-11 | Ablynx N.V. | Pseudomonas aeruginosa PCRV binding single variable domain antibodies |
JP2019013239A (ja) * | 2012-03-02 | 2019-01-31 | アブリンクス エン.ヴェー. | シュードモナス・エルギノーサのPcrVに結合する単一可変ドメイン抗体 |
JP7007250B2 (ja) | 2012-03-02 | 2022-02-10 | アブリンクス エン.ヴェー. | シュードモナス・エルギノーサのPcrVに結合する単一可変ドメイン抗体 |
US11312788B2 (en) | 2012-03-02 | 2022-04-26 | Ablynx N.V. | Pseudomonas aeruginosa PcrV binding single variable domain antibodies |
WO2020252029A1 (en) | 2019-06-11 | 2020-12-17 | Regeneron Pharmaceuticals, Inc. | Anti-pcrv antibodies that bind pcrv, compositions comprising anti-pcrv antibodies, and methods of use thereof |
US11655286B2 (en) | 2019-06-11 | 2023-05-23 | Regeneron Pharmaceuticals, Inc. | Anti-PcrV antibodies that bind PcrV, compositions comprising anti-PcrV antibodies, and methods of use thereof |
Also Published As
Publication number | Publication date |
---|---|
EP2248826A4 (en) | 2011-03-02 |
JPWO2009088032A1 (ja) | 2011-05-26 |
KR20100103675A (ko) | 2010-09-27 |
AU2009203426B2 (en) | 2011-11-24 |
KR101259239B1 (ko) | 2013-04-29 |
JP4766716B2 (ja) | 2011-09-07 |
AU2009203426A1 (en) | 2009-07-16 |
PT2248826E (pt) | 2013-09-06 |
US20110150896A1 (en) | 2011-06-23 |
US8501179B2 (en) | 2013-08-06 |
BRPI0906810A2 (pt) | 2019-10-01 |
PL2248826T3 (pl) | 2013-11-29 |
EA201070826A1 (ru) | 2011-04-29 |
EP2248826A1 (en) | 2010-11-10 |
ES2426093T3 (es) | 2013-10-21 |
EP2248826B1 (en) | 2013-06-12 |
EA020544B1 (ru) | 2014-12-30 |
CA2711863C (en) | 2014-06-10 |
CA2711863A1 (en) | 2009-07-16 |
CN102137870B (zh) | 2014-09-03 |
CN102137870A (zh) | 2011-07-27 |
EP2599792A1 (en) | 2013-06-05 |
AU2009203426C1 (en) | 2012-05-31 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
US11512129B2 (en) | TIGIT antibody, antigen-binding fragment thereof, and medical use thereof | |
JP4766716B2 (ja) | PcrVに対する抗体 | |
WO2017219995A1 (zh) | Lag-3抗体、其抗原结合片段及其医药用途 | |
US20200347130A1 (en) | CD96 Antibody, Antigen-Binding Fragment and Pharmaceutical use Thereof | |
WO2019024911A1 (zh) | B7h3抗体-药物偶联物及其医药用途 | |
JP5780595B2 (ja) | 抗緑膿菌作用を有するヒト化PcrV抗体 | |
JP2023166465A (ja) | 抗ネクチン-4抗体、それを含むコンジュゲート、及びその適用 | |
JPWO2016039402A1 (ja) | 血管内皮リパーゼの酵素活性を阻害するヒト化モノクローナル抗体 | |
KR101854529B1 (ko) | 인간 및 마우스 Sema3A에 교차결합하는 항체 및 그의 용도 | |
KR20150127248A (ko) | 혈관내피 리파제의 효소 활성을 저해하는 모노클로널 항체 | |
RU2822550C2 (ru) | Антитело против клаудина 18.2 и его применение | |
WO2022078424A1 (zh) | 抗trop-2抗体、其抗原结合片段或其突变体、及其医药用途 | |
JP2022523750A (ja) | 抗-fgf19抗体 |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
WWE | Wipo information: entry into national phase |
Ref document number: 200980109395.6 Country of ref document: CN |
|
121 | Ep: the epo has been informed by wipo that ep was designated in this application |
Ref document number: 09701235 Country of ref document: EP Kind code of ref document: A1 |
|
DPE1 | Request for preliminary examination filed after expiration of 19th month from priority date (pct application filed from 20040101) | ||
ENP | Entry into the national phase |
Ref document number: 2009548942 Country of ref document: JP Kind code of ref document: A |
|
WWE | Wipo information: entry into national phase |
Ref document number: 2009203426 Country of ref document: AU |
|
WWE | Wipo information: entry into national phase |
Ref document number: MX/A/2010/007522 Country of ref document: MX |
|
WWE | Wipo information: entry into national phase |
Ref document number: 2711863 Country of ref document: CA |
|
NENP | Non-entry into the national phase |
Ref country code: DE |
|
WWE | Wipo information: entry into national phase |
Ref document number: 12863983 Country of ref document: US |
|
ENP | Entry into the national phase |
Ref document number: 2009203426 Country of ref document: AU Date of ref document: 20090108 Kind code of ref document: A |
|
WWE | Wipo information: entry into national phase |
Ref document number: 4822/CHENP/2010 Country of ref document: IN |
|
WWE | Wipo information: entry into national phase |
Ref document number: 2009701235 Country of ref document: EP Ref document number: 201070826 Country of ref document: EA |
|
ENP | Entry into the national phase |
Ref document number: 20107017717 Country of ref document: KR Kind code of ref document: A |
|
ENP | Entry into the national phase |
Ref document number: PI0906810 Country of ref document: BR Kind code of ref document: A2 Effective date: 20100709 |