WO2009060857A1 - 膜タンパク質の製造方法 - Google Patents
膜タンパク質の製造方法 Download PDFInfo
- Publication number
- WO2009060857A1 WO2009060857A1 PCT/JP2008/070116 JP2008070116W WO2009060857A1 WO 2009060857 A1 WO2009060857 A1 WO 2009060857A1 JP 2008070116 W JP2008070116 W JP 2008070116W WO 2009060857 A1 WO2009060857 A1 WO 2009060857A1
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- membrane protein
- surfactant
- concentration
- liposome
- reaction liquid
- Prior art date
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/10—Dispersions; Emulsions
- A61K9/127—Liposomes
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P17/00—Drugs for dermatological disorders
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P3/00—Drugs for disorders of the metabolism
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/04—Antibacterial agents
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P35/00—Antineoplastic agents
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P37/00—Drugs for immunological or allergic disorders
- A61P37/02—Immunomodulators
- A61P37/04—Immunostimulants
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- General Chemical & Material Sciences (AREA)
- Medicinal Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Pharmacology & Pharmacy (AREA)
- Engineering & Computer Science (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Immunology (AREA)
- Biochemistry (AREA)
- Epidemiology (AREA)
- Dispersion Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Genetics & Genomics (AREA)
- General Engineering & Computer Science (AREA)
- Communicable Diseases (AREA)
- Oncology (AREA)
- Diabetes (AREA)
- Obesity (AREA)
- Hematology (AREA)
- Dermatology (AREA)
- Peptides Or Proteins (AREA)
- Medicinal Preparation (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
脂質ディスク又はリポソーム内で天然構造又は活性構造に折りたたまれた膜タンパク質の製造方法を提供する。(a)膜タンパク質をコードするポリヌクレオチドと、ステロイド性界面活性剤と、リン脂質と、を含む無細胞タンパク質合成反応液を調製する工程、ここで、前記ステロイド性界面活性剤は、臨界ミセル濃度よりも高い濃度で含まれ、(b)前記反応液の界面活性剤濃度を低下させる工程、及び(c)前記膜タンパク質を合成すると共に、当該膜タンパク質を組み込んだ脂質ディスク又はリポソームを形成させる工程、を含む。
Priority Applications (4)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
DK08846826.9T DK2213747T3 (da) | 2007-11-05 | 2008-11-05 | Fremgangsmåde til fremstilling af membranprotein |
EP08846826.9A EP2213747B1 (en) | 2007-11-05 | 2008-11-05 | Method for producing membrane protein |
JP2009540056A JP5704677B2 (ja) | 2007-11-05 | 2008-11-05 | 膜タンパク質の製造方法 |
US12/741,575 US8226976B2 (en) | 2007-11-05 | 2008-11-05 | Method of using cell-free protein synthesis to produce a membrane protein |
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP2007286899 | 2007-11-05 | ||
JP2007-286899 | 2007-11-05 |
Publications (1)
Publication Number | Publication Date |
---|---|
WO2009060857A1 true WO2009060857A1 (ja) | 2009-05-14 |
Family
ID=40625745
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/JP2008/070116 WO2009060857A1 (ja) | 2007-11-05 | 2008-11-05 | 膜タンパク質の製造方法 |
Country Status (5)
Country | Link |
---|---|
US (1) | US8226976B2 (ja) |
EP (1) | EP2213747B1 (ja) |
JP (1) | JP5704677B2 (ja) |
DK (1) | DK2213747T3 (ja) |
WO (1) | WO2009060857A1 (ja) |
Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2017022696A1 (ja) * | 2015-07-31 | 2017-02-09 | 国立研究開発法人理化学研究所 | 膜タンパク質の製造方法およびその利用 |
JP2018111079A (ja) * | 2017-01-13 | 2018-07-19 | 国立大学法人名古屋大学 | 脂質二重膜構造体とその製造方法 |
JP2018139563A (ja) * | 2017-02-28 | 2018-09-13 | 東洋紡株式会社 | Rnアーゼ阻害剤組成物 |
Families Citing this family (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US10961286B2 (en) | 2014-08-15 | 2021-03-30 | Cornell University | Nucleic acids, vectors, host cells, and methods for recombinantly producing water-soluble membrane proteins |
US10220378B2 (en) * | 2017-06-01 | 2019-03-05 | Uchicago Argonne, Llc | Semiconductor-metal nanoparticle hybrids with natural and artificial proton pump for hydrogen production |
US11001868B2 (en) | 2017-08-11 | 2021-05-11 | Global Life Sciences Solutions Operations UK Ltd | Cell-free protein expression using double-stranded concatameric DNA |
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JP2006219401A (ja) | 2005-02-09 | 2006-08-24 | Toray Ind Inc | 受容体を内包した分子会合体結晶とその製造方法およびその利用方法 |
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2008
- 2008-11-05 JP JP2009540056A patent/JP5704677B2/ja active Active
- 2008-11-05 US US12/741,575 patent/US8226976B2/en active Active
- 2008-11-05 DK DK08846826.9T patent/DK2213747T3/da active
- 2008-11-05 WO PCT/JP2008/070116 patent/WO2009060857A1/ja active Application Filing
- 2008-11-05 EP EP08846826.9A patent/EP2213747B1/en active Active
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JP2000175695A (ja) | 1998-12-14 | 2000-06-27 | Inst Of Physical & Chemical Res | 無細胞タンパク質合成系によるポリペプチドの製造方法 |
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Cited By (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2017022696A1 (ja) * | 2015-07-31 | 2017-02-09 | 国立研究開発法人理化学研究所 | 膜タンパク質の製造方法およびその利用 |
JPWO2017022696A1 (ja) * | 2015-07-31 | 2018-05-24 | 国立研究開発法人理化学研究所 | 膜タンパク質の製造方法およびその利用 |
US11618777B2 (en) | 2015-07-31 | 2023-04-04 | Shigeyuki Yokoyama | Method of manufacturing membrane protein and utilization thereof |
JP2018111079A (ja) * | 2017-01-13 | 2018-07-19 | 国立大学法人名古屋大学 | 脂質二重膜構造体とその製造方法 |
JP2018139563A (ja) * | 2017-02-28 | 2018-09-13 | 東洋紡株式会社 | Rnアーゼ阻害剤組成物 |
JP2021100965A (ja) * | 2017-02-28 | 2021-07-08 | 東洋紡株式会社 | Rnアーゼ阻害剤組成物 |
JP7036259B2 (ja) | 2017-02-28 | 2022-03-15 | 東洋紡株式会社 | Rnアーゼ阻害剤組成物 |
Also Published As
Publication number | Publication date |
---|---|
US20100291189A1 (en) | 2010-11-18 |
DK2213747T3 (da) | 2021-08-09 |
EP2213747B1 (en) | 2021-06-30 |
US8226976B2 (en) | 2012-07-24 |
JPWO2009060857A1 (ja) | 2011-03-24 |
JP5704677B2 (ja) | 2015-04-22 |
EP2213747A1 (en) | 2010-08-04 |
EP2213747A4 (en) | 2012-02-22 |
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