WO2009060857A1 - 膜タンパク質の製造方法 - Google Patents

膜タンパク質の製造方法 Download PDF

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Publication number
WO2009060857A1
WO2009060857A1 PCT/JP2008/070116 JP2008070116W WO2009060857A1 WO 2009060857 A1 WO2009060857 A1 WO 2009060857A1 JP 2008070116 W JP2008070116 W JP 2008070116W WO 2009060857 A1 WO2009060857 A1 WO 2009060857A1
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WIPO (PCT)
Prior art keywords
membrane protein
surfactant
concentration
liposome
reaction liquid
Prior art date
Application number
PCT/JP2008/070116
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English (en)
French (fr)
Inventor
Shigeyuki Yokoyama
Kazumi Shimono
Mikako Shirouzu
Mie Goto
Original Assignee
Riken
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Publication date
Application filed by Riken filed Critical Riken
Priority to DK08846826.9T priority Critical patent/DK2213747T3/da
Priority to US12/741,575 priority patent/US8226976B2/en
Priority to JP2009540056A priority patent/JP5704677B2/ja
Priority to EP08846826.9A priority patent/EP2213747B1/en
Publication of WO2009060857A1 publication Critical patent/WO2009060857A1/ja

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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K9/00—Medicinal preparations characterised by special physical form
    • A61K9/10—Dispersions; Emulsions
    • A61K9/127—Liposomes
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P17/00—Drugs for dermatological disorders
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P3/00—Drugs for disorders of the metabolism
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/04—Antibacterial agents
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P35/00—Antineoplastic agents
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P37/00—Drugs for immunological or allergic disorders
    • A61P37/02—Immunomodulators
    • A61P37/04—Immunostimulants
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Animal Behavior & Ethology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Engineering & Computer Science (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Immunology (AREA)
  • Biochemistry (AREA)
  • Dispersion Chemistry (AREA)
  • Epidemiology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Genetics & Genomics (AREA)
  • General Engineering & Computer Science (AREA)
  • Communicable Diseases (AREA)
  • Oncology (AREA)
  • Diabetes (AREA)
  • Dermatology (AREA)
  • Hematology (AREA)
  • Obesity (AREA)
  • Peptides Or Proteins (AREA)
  • Medicinal Preparation (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

　脂質ディスク又はリポソーム内で天然構造又は活性構造に折りたたまれた膜タンパク質の製造方法を提供する。（ａ）膜タンパク質をコードするポリヌクレオチドと、ステロイド性界面活性剤と、リン脂質と、を含む無細胞タンパク質合成反応液を調製する工程、ここで、前記ステロイド性界面活性剤は、臨界ミセル濃度よりも高い濃度で含まれ、（ｂ）前記反応液の界面活性剤濃度を低下させる工程、及び（ｃ）前記膜タンパク質を合成すると共に、当該膜タンパク質を組み込んだ脂質ディスク又はリポソームを形成させる工程、を含む。
PCT/JP2008/070116 2007-11-05 2008-11-05 膜タンパク質の製造方法 WO2009060857A1 (ja)

Priority Applications (4)

Application Number Priority Date Filing Date Title
DK08846826.9T DK2213747T3 (da) 2007-11-05 2008-11-05 Fremgangsmåde til fremstilling af membranprotein
US12/741,575 US8226976B2 (en) 2007-11-05 2008-11-05 Method of using cell-free protein synthesis to produce a membrane protein
JP2009540056A JP5704677B2 (ja) 2007-11-05 2008-11-05 膜タンパク質の製造方法
EP08846826.9A EP2213747B1 (en) 2007-11-05 2008-11-05 Method for producing membrane protein

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2007-286899 2007-11-05
JP2007286899 2007-11-05

Publications (1)

Publication Number Publication Date
WO2009060857A1 true WO2009060857A1 (ja) 2009-05-14

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Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/JP2008/070116 WO2009060857A1 (ja) 2007-11-05 2008-11-05 膜タンパク質の製造方法

Country Status (5)

Country Link
US (1) US8226976B2 (ja)
EP (1) EP2213747B1 (ja)
JP (1) JP5704677B2 (ja)
DK (1) DK2213747T3 (ja)
WO (1) WO2009060857A1 (ja)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2017022696A1 (ja) * 2015-07-31 2017-02-09 国立研究開発法人理化学研究所 膜タンパク質の製造方法およびその利用
JP2018111079A (ja) * 2017-01-13 2018-07-19 国立大学法人名古屋大学 脂質二重膜構造体とその製造方法
JP2018139563A (ja) * 2017-02-28 2018-09-13 東洋紡株式会社 Ｒｎアーゼ阻害剤組成物

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10961286B2 (en) 2014-08-15 2021-03-30 Cornell University Nucleic acids, vectors, host cells, and methods for recombinantly producing water-soluble membrane proteins
US10220378B2 (en) * 2017-06-01 2019-03-05 Uchicago Argonne, Llc Semiconductor-metal nanoparticle hybrids with natural and artificial proton pump for hydrogen production
US11001868B2 (en) 2017-08-11 2021-05-11 Global Life Sciences Solutions Operations UK Ltd Cell-free protein expression using double-stranded concatameric DNA

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Cited By (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2017022696A1 (ja) * 2015-07-31 2017-02-09 国立研究開発法人理化学研究所 膜タンパク質の製造方法およびその利用
JPWO2017022696A1 (ja) * 2015-07-31 2018-05-24 国立研究開発法人理化学研究所 膜タンパク質の製造方法およびその利用
US11618777B2 (en) 2015-07-31 2023-04-04 Shigeyuki Yokoyama Method of manufacturing membrane protein and utilization thereof
JP2018111079A (ja) * 2017-01-13 2018-07-19 国立大学法人名古屋大学 脂質二重膜構造体とその製造方法
JP2018139563A (ja) * 2017-02-28 2018-09-13 東洋紡株式会社 Ｒｎアーゼ阻害剤組成物
JP2021100965A (ja) * 2017-02-28 2021-07-08 東洋紡株式会社 Ｒｎアーゼ阻害剤組成物
JP7036259B2 (ja) 2017-02-28 2022-03-15 東洋紡株式会社 Ｒｎアーゼ阻害剤組成物

Also Published As

Publication number Publication date
EP2213747A4 (en) 2012-02-22
JPWO2009060857A1 (ja) 2011-03-24
US8226976B2 (en) 2012-07-24
US20100291189A1 (en) 2010-11-18
EP2213747A1 (en) 2010-08-04
DK2213747T3 (da) 2021-08-09
EP2213747B1 (en) 2021-06-30
JP5704677B2 (ja) 2015-04-22

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