WO2008123112A1 - 核酸検出カセット及び核酸検出装置 - Google Patents

核酸検出カセット及び核酸検出装置 Download PDF

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Publication number
WO2008123112A1
WO2008123112A1 PCT/JP2008/055134 JP2008055134W WO2008123112A1 WO 2008123112 A1 WO2008123112 A1 WO 2008123112A1 JP 2008055134 W JP2008055134 W JP 2008055134W WO 2008123112 A1 WO2008123112 A1 WO 2008123112A1
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WIPO (PCT)
Prior art keywords
nucleic acid
acid detection
heat treatment
treatment chamber
cassette
Prior art date
Application number
PCT/JP2008/055134
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English (en)
French (fr)
Inventor
Sadato Hongo
Takahiro Kokubo
Hideki Horiuchi
Koji Hashimoto
Jun Okada
Original Assignee
Kabushiki Kaisha Toshiba
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Kabushiki Kaisha Toshiba filed Critical Kabushiki Kaisha Toshiba
Priority to EP08722509A priority Critical patent/EP2071026A1/en
Priority to US12/175,978 priority patent/US20090023201A1/en
Publication of WO2008123112A1 publication Critical patent/WO2008123112A1/ja

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    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502738—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by integrated valves
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/50273—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the means or forces applied to move the fluids
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L7/00—Heating or cooling apparatus; Heat insulating devices
    • B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0621—Control of the sequence of chambers filled or emptied
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/10—Integrating sample preparation and analysis in single entity, e.g. lab-on-a-chip concept
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/12—Specific details about manufacturing devices
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/14—Process control and prevention of errors
    • B01L2200/142—Preventing evaporation
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/06—Auxiliary integrated devices, integrated components
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/06—Auxiliary integrated devices, integrated components
    • B01L2300/0627—Sensor or part of a sensor is integrated
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/06—Auxiliary integrated devices, integrated components
    • B01L2300/0627—Sensor or part of a sensor is integrated
    • B01L2300/0645—Electrodes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
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    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0816—Cards, e.g. flat sample carriers usually with flow in two horizontal directions
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • B01L2300/0867—Multiple inlets and one sample wells, e.g. mixing, dilution
    • B—PERFORMING OPERATIONS; TRANSPORTING
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    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
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    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
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    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • B01L2300/0874—Three dimensional network
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    • B01L2300/0887—Laminated structure
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    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
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    • B01L2300/12—Specific details about materials
    • B01L2300/123—Flexible; Elastomeric
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/18—Means for temperature control
    • B01L2300/1805—Conductive heating, heat from thermostatted solids is conducted to receptacles, e.g. heating plates, blocks
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/18—Means for temperature control
    • B01L2300/1805—Conductive heating, heat from thermostatted solids is conducted to receptacles, e.g. heating plates, blocks
    • B01L2300/1827—Conductive heating, heat from thermostatted solids is conducted to receptacles, e.g. heating plates, blocks using resistive heater
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
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    • B01L2300/1883—Means for temperature control using thermal insulation
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    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0475—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure
    • B01L2400/0487—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure fluid pressure, pneumatics
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/06—Valves, specific forms thereof
    • B01L2400/0633—Valves, specific forms thereof with moving parts
    • B01L2400/0655—Valves, specific forms thereof with moving parts pinch valves
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N2035/00346—Heating or cooling arrangements
    • G01N2035/00356—Holding samples at elevated temperature (incubation)
    • G01N2035/00366—Several different temperatures used

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Hematology (AREA)
  • Dispersion Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

　　　核酸検出カセットにおいては、加熱処理チャンバと送液流路とが固定部材及び可撓性部材により構成されている核酸検出カセットにおいて、加熱処理チャンバの両側から凸状加熱部が挟み込むことにより、加熱処理チャンバと送液流路とを空間的に分離する。従って、対象サンプルの核酸増幅及びその他の必要な処理と標的核酸の検出までの一貫した処理を自動的に行うことが出来る。
PCT/JP2008/055134 2007-03-23 2008-03-19 核酸検出カセット及び核酸検出装置 WO2008123112A1 (ja)

Priority Applications (2)

Application Number Priority Date Filing Date Title
EP08722509A EP2071026A1 (en) 2007-03-23 2008-03-19 Nucleic acid detection cassette and nucleic acid detection apparatus
US12/175,978 US20090023201A1 (en) 2007-03-23 2008-07-18 Nucleic acid detecting cassette and nucleic acid detecting apparatus

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
JP2007-077915 2007-03-23
JP2007077915 2007-03-23
JP2008070976A JP2008263959A (ja) 2007-03-23 2008-03-19 核酸検出カセット及び核酸検出装置
JP2008-070976 2008-03-19

Related Child Applications (1)

Application Number Title Priority Date Filing Date
US12/175,978 Continuation US20090023201A1 (en) 2007-03-23 2008-07-18 Nucleic acid detecting cassette and nucleic acid detecting apparatus

Publications (1)

Publication Number Publication Date
WO2008123112A1 true WO2008123112A1 (ja) 2008-10-16

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ID=40044366

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Application Number Title Priority Date Filing Date
PCT/JP2008/055134 WO2008123112A1 (ja) 2007-03-23 2008-03-19 核酸検出カセット及び核酸検出装置

Country Status (5)

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US (1) US20090023201A1 (ja)
EP (1) EP2071026A1 (ja)
JP (1) JP2008263959A (ja)
CN (1) CN101541962A (ja)
WO (1) WO2008123112A1 (ja)

Cited By (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2010040748A1 (de) * 2008-10-06 2010-04-15 Aj Ebiochip Gmbh Vorrichtung und verfahren zur handhabung von flüssigkeiten
WO2010080115A3 (en) * 2008-12-18 2010-09-16 Claros Diagnostics, Inc. Improved reagent storage in microfluidic systems and related articles and methods
JP2011062119A (ja) * 2009-09-16 2011-03-31 Seiko Epson Corp 生体試料定量用チップ
JP2011062197A (ja) * 2010-09-06 2011-03-31 Seiko Epson Corp 生体試料定量方法
USD645971S1 (en) 2010-05-11 2011-09-27 Claros Diagnostics, Inc. Sample cassette
US8030057B2 (en) 2004-01-26 2011-10-04 President And Fellows Of Harvard College Fluid delivery system and method
US8389272B2 (en) 2004-01-26 2013-03-05 President And Fellows Of Harvard College Fluid delivery system and method
US8409527B2 (en) 2007-05-04 2013-04-02 Opko Diagnostics, Llc Fluidic connectors and microfluidic systems
US8580569B2 (en) 2010-04-16 2013-11-12 Opko Diagnostics, Llc Feedback control in microfluidic systems
US8915259B2 (en) 2009-11-24 2014-12-23 Opko Diagnostics, Llc Fluid mixing and delivery in microfluidic systems
JP2015518708A (ja) * 2012-05-08 2015-07-06 ノースウエスタン ユニバーシティ 試料から検体を隔離するための自動システムで使用するためのカートリッジ及び使用方法
US9255866B2 (en) 2013-03-13 2016-02-09 Opko Diagnostics, Llc Mixing of fluids in fluidic systems
WO2016121886A1 (ja) * 2015-01-28 2016-08-04 株式会社ダナフォーム 分析用デバイス、分析用チップ、分析キット、および、これらを用いた分析方法
US9827564B2 (en) 2009-02-02 2017-11-28 Opko Diagnostics, Llc Fluidic systems and methods for analyses
USD804682S1 (en) 2015-08-10 2017-12-05 Opko Diagnostics, Llc Multi-layered sample cassette
US10279345B2 (en) 2014-12-12 2019-05-07 Opko Diagnostics, Llc Fluidic systems comprising an incubation channel, including fluidic systems formed by molding
CN110283709A (zh) * 2019-05-09 2019-09-27 广州和实生物技术有限公司 全自动核酸分子杂交仪
US10501735B2 (en) 2012-08-23 2019-12-10 Quidel Corporation Device with controlled fluid dynamics, for isolation of an analyte from a sample
US10672503B2 (en) 2012-03-05 2020-06-02 Opko Diagnostics, Llc Methods and apparatuses for conducting analyses
CN114471751A (zh) * 2020-10-27 2022-05-13 京东方科技集团股份有限公司 检测芯片及其操作方法、检测装置

Families Citing this family (37)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7754148B2 (en) 2006-12-27 2010-07-13 Progentech Limited Instrument for cassette for sample preparation
US7727473B2 (en) 2005-10-19 2010-06-01 Progentech Limited Cassette for sample preparation
KR20160088958A (ko) 2010-02-23 2016-07-26 루미넥스 코포레이션 일체화된 샘플 제조, 반응 및 검출을 위한 장치 및 방법
DE102010003782B4 (de) 2010-04-08 2023-09-28 Ist Innuscreen Gmbh Vorrichtung zum Nachweis von Nukleinsäuren
JP5579537B2 (ja) * 2010-08-23 2014-08-27 株式会社堀場製作所 細胞分析用カートリッジ
FR2967362B1 (fr) * 2010-11-16 2012-12-21 Genewave Cartouche microfluidique pour diagnostic moleculaire
JPWO2012086168A1 (ja) * 2010-12-21 2014-05-22 日本電気株式会社 試料の加熱方法及び加熱制御装置
CN104023834B (zh) 2011-05-04 2016-09-28 卢米耐克斯公司 用于集成的样品制备、反应和检测的设备与方法
EP2740788A4 (en) 2011-08-05 2015-08-19 Toshiba Kk MULTIPLE NUCLEIC ACID AMPLIFICATION REACTION INSTRUMENT
US9145579B2 (en) * 2012-07-24 2015-09-29 Panasonic Healthcare Co., Ltd. Analyzing device
JP6081113B2 (ja) * 2012-09-19 2017-02-15 東芝メディカルシステムズ株式会社 核酸検出用デバイス及び核酸検出装置
KR101955330B1 (ko) 2012-12-21 2019-03-07 삼성전자주식회사 핵산 분석용 반응 시약 공급 장치
KR101446526B1 (ko) 2013-05-02 2014-10-08 주식회사 퀀타매트릭스 마이크로플루이딕 멀티-웰 기반의 세포배양검사 장치
KR101396110B1 (ko) 2013-10-30 2014-05-16 아주대학교산학협력단 장쇄 분지를 갖는 지방족 폴리카보네이트 및 이의 방향족 폴리에스터 공중합체
JP6855154B2 (ja) * 2014-09-02 2021-04-07 キヤノンメディカルシステムズ株式会社 核酸検出カセット
WO2016035817A1 (ja) 2014-09-02 2016-03-10 株式会社 東芝 核酸検出カセット
CN104911095B (zh) * 2015-06-16 2017-07-07 东南大学 一种流体卡盒
US20190022637A1 (en) * 2015-09-04 2019-01-24 Life Technologies Corporation Devices and Methods for Mesofluidic and/or Microfluidic Processes
US10852310B2 (en) 2015-12-11 2020-12-01 Opko Diagnostics, Llc Fluidic systems involving incubation of samples and/or reagents
TWI789343B (zh) 2016-02-01 2023-01-11 丹麥商碩騰丹麥有限公司 微流體分析系統、執行分析的微流體匣及方法
WO2017213123A1 (ja) * 2016-06-07 2017-12-14 国立大学法人東京大学 流体デバイス
US10682644B2 (en) 2016-10-07 2020-06-16 Boehringer Ingelheim Vetmedica Gmbh Cartridge, analysis system and method for testing a sample
GB201617869D0 (en) 2016-10-21 2016-12-07 Blacktrace Holdings Limited A microfluidic device
USD862725S1 (en) * 2016-11-03 2019-10-08 This Ag Cassette for ophthalmologic apparatus
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USD834721S1 (en) * 2017-03-03 2018-11-27 Neogen Corporation Assay cartridge
AU2018255430B2 (en) * 2017-04-21 2022-12-08 Mesa Biotech, Inc. Fluidic test cassette
WO2019146190A1 (ja) * 2018-01-29 2019-08-01 コニカミノルタ株式会社 温調ステージ
KR102105558B1 (ko) * 2018-03-23 2020-04-28 (주)바이오니아 고속 중합효소 연쇄반응 분석 플레이트
US10974240B2 (en) * 2018-07-06 2021-04-13 Qorvo Us, Inc. Fluidic channel for a cartridge
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US11964271B2 (en) * 2022-07-27 2024-04-23 Pilot Gene Technology (hangzhou) Co., Ltd. Drop preparation device and drop preparation method

Citations (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH037571A (ja) 1989-02-03 1991-01-14 Eastman Kodak Co Ｐｃｒ用の封入キュベットおよびその使用方法
JPH05261298A (ja) 1992-03-24 1993-10-12 Toray Ind Inc 固体酸触媒
JP2573443B2 (ja) 1990-09-28 1997-01-22 株式会社東芝 遺伝子検出法
US5776672A (en) 1990-09-28 1998-07-07 Kabushiki Kaisha Toshiba Gene detection method
JP2002223393A (ja) 2000-11-27 2002-08-09 Sanyo Electric Co Ltd 電荷転送素子
JP2003517591A (ja) * 1999-12-09 2003-05-27 モトローラ・インコーポレイテッド 分析試料の反応を行うための多層微量流体デバイス
JP2003200440A (ja) 2002-01-08 2003-07-15 Canon Chemicals Inc ゴムローラの成形金型、ゴムローラの製造方法
JP2005176836A (ja) * 2003-11-28 2005-07-07 Toshiba Tec Corp 核酸検出カセット、核酸検出装置及び核酸検出システム
JP2005261298A (ja) * 2004-03-18 2005-09-29 Toshiba Corp 核酸検出カセット及び核酸検出装置
JP2005323519A (ja) * 2004-05-13 2005-11-24 Konica Minolta Sensing Inc マイクロ流体デバイス並びに試液の試験方法および試験システム
JP2006262787A (ja) * 2005-03-24 2006-10-05 Toshiba Tec Corp 核酸検出カセット及びカセット注入システム
JP2006262788A (ja) * 2005-03-24 2006-10-05 Toshiba Tec Corp 反応装置
WO2007111274A1 (ja) * 2006-03-24 2007-10-04 Kabushiki Kaisha Toshiba 核酸検出カセット及び核酸検出装置

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US2008A (en) * 1841-03-18 Gas-lamp eok conducting gas pkom ah elevated buhner to one below it
US2006A (en) * 1841-03-16 Clamp for crimping leather
US2004A (en) * 1841-03-12 Improvement in the manner of constructing and propelling steam-vessels
US2005A (en) * 1841-03-16 Improvement in the manner of constructing molds for casting butt-hinges
US6572830B1 (en) * 1998-10-09 2003-06-03 Motorola, Inc. Integrated multilayered microfludic devices and methods for making the same
JP4286167B2 (ja) * 2004-03-17 2009-06-24 株式会社シー・アイ・シー 小動物捕獲器

Patent Citations (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH037571A (ja) 1989-02-03 1991-01-14 Eastman Kodak Co Ｐｃｒ用の封入キュベットおよびその使用方法
JP2573443B2 (ja) 1990-09-28 1997-01-22 株式会社東芝 遺伝子検出法
US5776672A (en) 1990-09-28 1998-07-07 Kabushiki Kaisha Toshiba Gene detection method
US5972692A (en) 1990-09-28 1999-10-26 Kabushiki Kaisha Toshiba Gene detection method
JPH05261298A (ja) 1992-03-24 1993-10-12 Toray Ind Inc 固体酸触媒
JP2003517591A (ja) * 1999-12-09 2003-05-27 モトローラ・インコーポレイテッド 分析試料の反応を行うための多層微量流体デバイス
JP2002223393A (ja) 2000-11-27 2002-08-09 Sanyo Electric Co Ltd 電荷転送素子
JP2003200440A (ja) 2002-01-08 2003-07-15 Canon Chemicals Inc ゴムローラの成形金型、ゴムローラの製造方法
JP2005176836A (ja) * 2003-11-28 2005-07-07 Toshiba Tec Corp 核酸検出カセット、核酸検出装置及び核酸検出システム
JP2005261298A (ja) * 2004-03-18 2005-09-29 Toshiba Corp 核酸検出カセット及び核酸検出装置
JP2005323519A (ja) * 2004-05-13 2005-11-24 Konica Minolta Sensing Inc マイクロ流体デバイス並びに試液の試験方法および試験システム
JP2006262787A (ja) * 2005-03-24 2006-10-05 Toshiba Tec Corp 核酸検出カセット及びカセット注入システム
JP2006262788A (ja) * 2005-03-24 2006-10-05 Toshiba Tec Corp 反応装置
WO2007111274A1 (ja) * 2006-03-24 2007-10-04 Kabushiki Kaisha Toshiba 核酸検出カセット及び核酸検出装置

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Publication number Priority date Publication date Assignee Title
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US8389272B2 (en) 2004-01-26 2013-03-05 President And Fellows Of Harvard College Fluid delivery system and method
US10048252B2 (en) 2004-01-26 2018-08-14 President And Fellows Of Harvard College Fluid delivery system and method
US8802445B2 (en) 2007-05-04 2014-08-12 Opko Diagnostics, Llc Fluidic connectors and microfluidic systems
US10775369B2 (en) 2007-05-04 2020-09-15 Opko Diagnostics, Llc Fluidic systems for analyses
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US9234888B2 (en) 2007-05-04 2016-01-12 Opko Diagnostics, Llc Fluidic connectors and microfluidic systems
WO2010040748A1 (de) * 2008-10-06 2010-04-15 Aj Ebiochip Gmbh Vorrichtung und verfahren zur handhabung von flüssigkeiten
US8591829B2 (en) 2008-12-18 2013-11-26 Opko Diagnostics, Llc Reagent storage in microfluidic systems and related articles and methods
US9878324B2 (en) 2008-12-18 2018-01-30 Opko Diagnostics, Llc Reagent storage in microfluidic systems and related articles and methods
US9561506B2 (en) 2008-12-18 2017-02-07 Opko Diagnostics, Llc Reagent storage in microfluidic systems and related articles and methods
US10589265B2 (en) 2008-12-18 2020-03-17 Opko Diagnostics, Llc Reagent storage in microfluidic systems and related articles and methods
WO2010080115A3 (en) * 2008-12-18 2010-09-16 Claros Diagnostics, Inc. Improved reagent storage in microfluidic systems and related articles and methods
US9827563B2 (en) 2009-02-02 2017-11-28 Opko Diagnostics, Llc Fluidic systems and methods for analyses
US9827564B2 (en) 2009-02-02 2017-11-28 Opko Diagnostics, Llc Fluidic systems and methods for analyses
JP2011062119A (ja) * 2009-09-16 2011-03-31 Seiko Epson Corp 生体試料定量用チップ
US9555408B2 (en) 2009-11-24 2017-01-31 Opko Diagnostics, Llc Fluid mixing and delivery in microfluidic systems
US9731291B2 (en) 2009-11-24 2017-08-15 Opko Diagnostics, Llc Fluid mixing and delivery in microfluidic systems
US10953398B2 (en) 2009-11-24 2021-03-23 Opko Diagnostics, Llc Fluid mixing and delivery in microfluidic systems
US10413899B2 (en) 2009-11-24 2019-09-17 Opko Diagnostics, Llc Fluid mixing and delivery in microfluidic systems
US9075051B2 (en) 2009-11-24 2015-07-07 Opko Diagnostics, Llc Fluid mixing and delivery in microfluidic systems
US8915259B2 (en) 2009-11-24 2014-12-23 Opko Diagnostics, Llc Fluid mixing and delivery in microfluidic systems
US9861980B2 (en) 2009-11-24 2018-01-09 Opko Diagnostics, Llc Fluid mixing and delivery in microfluidic systems
US9981266B2 (en) 2010-04-16 2018-05-29 Opko Diagnostics, Llc Feedback control in microfluidic systems
US8765062B2 (en) 2010-04-16 2014-07-01 Opko Diagnostics, Llc Systems and devices for analysis of samples
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US9682376B2 (en) 2010-04-16 2017-06-20 Opko Diagnostics, Llc Systems and devices for analysis of samples
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US10456784B2 (en) 2010-04-16 2019-10-29 Opko Diagnostics, Llc Systems and devices for analysis of samples
US9116124B2 (en) 2010-04-16 2015-08-25 Opko Diagnostics, Llc Feedback control in microfluidic systems
US8580569B2 (en) 2010-04-16 2013-11-12 Opko Diagnostics, Llc Feedback control in microfluidic systems
USD645971S1 (en) 2010-05-11 2011-09-27 Claros Diagnostics, Inc. Sample cassette
JP2011062197A (ja) * 2010-09-06 2011-03-31 Seiko Epson Corp 生体試料定量方法
US10672503B2 (en) 2012-03-05 2020-06-02 Opko Diagnostics, Llc Methods and apparatuses for conducting analyses
JP2015518708A (ja) * 2012-05-08 2015-07-06 ノースウエスタン ユニバーシティ 試料から検体を隔離するための自動システムで使用するためのカートリッジ及び使用方法
US10501735B2 (en) 2012-08-23 2019-12-10 Quidel Corporation Device with controlled fluid dynamics, for isolation of an analyte from a sample
US9255866B2 (en) 2013-03-13 2016-02-09 Opko Diagnostics, Llc Mixing of fluids in fluidic systems
US9588027B2 (en) 2013-03-13 2017-03-07 UPKO Diagnostics, LLC Mixing of fluids in fluidic systems
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WO2016121886A1 (ja) * 2015-01-28 2016-08-04 株式会社ダナフォーム 分析用デバイス、分析用チップ、分析キット、および、これらを用いた分析方法
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