WO2006108345A1 - Poudre de vinaigre de fruit et d'œuf et préparation contenant une poudre de vinaigre et d'œuf - Google Patents

Poudre de vinaigre de fruit et d'œuf et préparation contenant une poudre de vinaigre et d'œuf Download PDF

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Publication number
WO2006108345A1
WO2006108345A1 PCT/CN2006/000594 CN2006000594W WO2006108345A1 WO 2006108345 A1 WO2006108345 A1 WO 2006108345A1 CN 2006000594 W CN2006000594 W CN 2006000594W WO 2006108345 A1 WO2006108345 A1 WO 2006108345A1
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Prior art keywords
vinegar
fruit vinegar
egg powder
egg
vinegar egg
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PCT/CN2006/000594
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English (en)
Chinese (zh)
Inventor
Xinfu Guo
Zhao Wang
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Zhejiang Hangzhou Xinfu Pharmaceutical Co., Ltd.
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Publication of WO2006108345A1 publication Critical patent/WO2006108345A1/fr

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    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L15/00—Egg products; Preparation or treatment thereof
    • A23L15/30—Addition of substances other than those covered by A23L15/20 – A23L15/25
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L15/00—Egg products; Preparation or treatment thereof
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P19/00—Drugs for skeletal disorders
    • A61P19/08—Drugs for skeletal disorders for bone diseases, e.g. rachitism, Paget's disease
    • A61P19/10—Drugs for skeletal disorders for bone diseases, e.g. rachitism, Paget's disease for osteoporosis
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P37/00—Drugs for immunological or allergic disorders
    • A61P37/02—Immunomodulators

Definitions

  • the present invention relates to a vinegar egg powder, a process for the preparation thereof, a composition containing vinegar egg powder, and the use of the composition. Background technique
  • Osteoporosis is mainly characterized by a decrease in bone mineral density and loosening of the bone, which often leads to compression deformation of the vertebrae and easy fracture of the femoral neck and forearm wrist.
  • osteoporosis many factors are related to osteoporosis, such as genetics, endocrinology, lifestyle, and dietary nutrition.
  • calcium, phosphorus, vitamin D and protein are the most closely related, and the most studied is calcium nutrition.
  • Calcium is one of the major mineral components in bones and a prerequisite for normal bone growth.
  • a large number of clinical trials and epidemiological surveys have shown that bone loss due to inadequate calcium intake is a major risk factor for osteoporosis.
  • step 2 Put the egg into the fruit vinegar prepared in step 1.
  • the ratio of fruit vinegar to egg is 3 ⁇ 5: 1, soak, stir well, and press-filter to obtain fruit vinegar egg liquid;
  • the present invention also provides a method for preparing the vinegar egg powder, comprising the following steps:
  • step 2 Put the egg into the fruit vinegar prepared in step 1.
  • the ratio of fruit vinegar to egg is 3 ⁇ 5: 1, soak, stir well, and press-filter to obtain fruit vinegar egg liquid;
  • Another object of the present invention is to provide a composition of vinegar egg powder which is made of the following raw materials: the above-mentioned fruit vinegar powder, calcium carbonate, epimedium, bone crush, huangjing, angelica, and notoginseng , casein phosphopeptide.
  • composition of the vinegar egg powder of the present invention is prepared from the following raw materials by weight ratio: 0.3-60% of the above-mentioned fruit vinegar powder, 5-50% of calcium carbonate, 5-60% of epimedium, bone Crushed 5-60%, Huangjing 5-55%, Angelica 5-50%, Sanqi 0.5-40%, Casein Phosphopeptide (CPP) 0.1-10%.
  • the composition of the vinegar egg powder of the present invention is prepared from the following raw materials by weight ratio: 0.3-40% of the above-mentioned fruit vinegar powder, 5-30% of calcium carbonate, 5-40% of epimedium, bone 5-40% of broken powder, 5-40% of Huangjing, 5-30% of Angelica, 1-30% of Sanqi, 0.1-10% of casein phosphopeptide. More preferably, the composition of the vinegar egg powder of the present invention is prepared from the following raw materials by weight ratio: 1-20% of the above-mentioned fruit vinegar powder, 10-20% of calcium carbonate, and 15-25% of epimedium. 15-25% of broken bones, 15-25% of Huangjing, 10-25% of Angelica, 5-15% of Panax notoginseng, and 0.1-5% of casein phosphopeptides.
  • Another object of the present invention is to provide the use of the above-described fruit vinegar powder composition for the preparation of a food for increasing bone density and preventing osteoporosis.
  • Another object of the present invention is to provide the use of the above-described vinegar egg powder composition for the preparation of a food for relieving fatigue and enhancing immunity.
  • Vinegar powder refers to egg products made by sterilizing eggs after being immersed in vinegar for a certain period of time, being crushed, filtered and dried.
  • the invention provides a fruit vinegar egg powder, which is prepared by soaking eggs in citrus or hawthorn juice,
  • the fruit vinegar obtained by fermentation, filtration and the like is prepared by mincing, pressure filtration and spray drying.
  • the preparation method of the vinegar egg powder is achieved by the following steps: washing the raw citrus or hawthorn, and processing into a juice by a crusher and a beater (0.5 mm aperture).
  • the juice is cooled to 28 ° C, and the activated yeast is added for fermentation for three days.
  • the fermentation liquid has an alcohol content of 6-7% (v/v) and the total sugar is reduced to less than 10 g/1, the fermentation is stopped to obtain citrus or hawthorn. wine.
  • the fermentation was stopped at -7%.
  • the cells were sterilized by pressure filtration using a plate and frame filter press, and sterilized by heating at 80 ° C for 15 minutes to obtain a citrus or hawthorn vinegar having a 6-8% acetic acid content. Put the washed, sterilized and sterilized eggs into the above-mentioned fruit vinegar.
  • the ratio of fruit vinegar to egg is 3 ⁇ 5:1, soak for 72 hours, until the pH value is about 5.
  • Egg liquid spray the fruit vinegar egg liquid to obtain fruit vinegar egg powder, the inlet air temperature is 150-160 ° C, and the outlet air temperature is 80-90 ° C.
  • the vinegar egg powder of the invention contains protein ⁇ 28.0 ⁇ / 100 ⁇ , calcium 3. 5 g / 100g, and is rich in B vitamins, amino acids and the like.
  • the present invention also provides a composition of vinegar egg powder, which is made from the following raw materials: the above-mentioned fruit vinegar powder, calcium carbonate, epimedium, sclerotium, huangjing, angelica, notoginseng, casein phosphate Peptide.
  • composition of the vinegar egg powder of the present invention is prepared from the following raw materials by weight ratio: 0.3-60% of the above-mentioned fruit vinegar powder, 5-50% of calcium carbonate, 5-60% of epimedium, bone Crushed 5-60%, Huangjing 5-55%, Angelica 5-50%, Sanqi 0.5-40%, Casein Phosphopeptide (CPP) 0.1-10%.
  • the composition of the vinegar egg powder of the present invention is prepared from the following raw materials by weight ratio: 0.3-40% of the above-mentioned fruit vinegar powder, 5-30% of calcium carbonate, 5-40% of epimedium, bone 5-40% of broken powder, 5-40% of Huangjing, 5-30% of Angelica, 1-30% of Sanqi, 0.1-10% of casein phosphopeptide. More preferably, the composition of the vinegar egg powder of the present invention is prepared from the following raw materials by weight ratio: 1-20% of the above-mentioned fruit vinegar powder, 10-20% of calcium carbonate, and 15-25% of epimedium. 15-25% of broken bones, 15-25% of Huangjing, 10-25% of Angelica, 5-15% of Panax notoginseng, and 0.1-5% of casein phosphopeptides.
  • composition of the vinegar egg powder of the invention is prepared by extracting the formula amount of Epimedium, Rhizoma Drynaria, Polygonatum, Angelica and Sanqi by water extraction and alcohol precipitation, concentrating into a thick paste, and then formulating the amount Made from vinegar egg powder, calcium carbonate, and casein phosphopeptide.
  • the extraction process of the traditional Chinese medicine component of the composition of the vinegar egg powder of the present invention is as follows: Epimedium, Drynaria, Polygonatum, Angelica, Sanqi water soaked, decocted twice, filtered, combined filtrate, concentrated into Clear Cream, add alcohol to the concentration of ethanol to 60%, let stand for 24 hours, filter, the filtrate is recovered ethanol, concentrated into a thick paste.
  • the present invention also provides the use of a composition of vinegar egg powder for the preparation of a health food product for increasing bone density, preventing osteoporosis, relieving fatigue, and enhancing immunity.
  • the preparation of the health food composition is usually in the form of a solid preparation, specifically a tablet or capsule or granule or granule.
  • the extraction of the active ingredients is carried out by a conventional production process, and the conventional excipients are added in different dosage forms and thoroughly mixed to form a finished product, which may be a tablet or a capsule or a granule or a granule.
  • a finished product which may be a tablet or a capsule or a granule or a granule.
  • the additional materials and the amount of the materials and the specific production processes are all known in the art, and will not be described herein.
  • the recommended amount of the composition of the present invention is: tablet, capsule preparation is 4.0 g of vinegar egg powder composition / person / day, granules, granules are 8.0 g of vinegar egg powder composition / person / day, orally once a day, Swallow or blister with water according to the dosage form.
  • composition of the present invention has no toxic and side effects, has an obvious effect of increasing bone density, and can be used for preparing a health food for increasing bone density.
  • the protein of the egg is a complete protein, which contains eight kinds of amino acids necessary for the human body. It is decomposed into peptides and amino acids to some extent during the soaking process with vinegar. In addition, there are lecithin, egg butter, vitamins, etc. Calcium absorption has a certain effect.
  • the vinegar contains sugar, lipids, proteins, amino acids, vitamins, bioflavonoids and minerals, especially the fruit, after the fermentation of vinegar, the B vitamins and other nutrients are significantly increased, which can make calcium in animal food.
  • the substance is dissolved and used by the human body to promote blood metabolism, eliminate fatigue and anti-aging effects. Studies have shown that eggs and egg skins are soaked and dried with 10% acetic acid, which is used as a calcium source to feed rats.
  • vinegar egg liquid can significantly promote intestinal peristalsis, promote digestive function, improve cellular immune function, and reduce lipid peroxidation in brain tissue.
  • Fruit vinegar egg powder has a supplemental calcium source in this side, increasing bone density, relieving fatigue, enhancing immunity, and strengthening stomach and digestion.
  • Calcium carbonate is a calcium supplement which is widely used in adult calcium supplementation. It has a small molecular weight and a high calcium content. It can quickly form soluble calcium ions when neutralizing gastric acid and is easily absorbed by the intestinal tract. Studies have shown that an appropriate increase in calcium intake can increase bone density, reduce bone loss, prevent fractures, and prevent osteoporosis. Animal experiments have shown that calcium carbonate has a good prevention of disuse osteoporosis in rats. It is more economical and reasonable compared with other calcium supplements. Therefore, calcium carbonate is used as a calcium source in this side, and it has calcium supplementation, increases bone density, and improves osteoporosis.
  • Epimedium The main active ingredient of Epimedium is the total flavonoids of Epimedium. Studies have shown that Epimedium aqueous extract inhibits osteoclasts, promotes osteoblast function, and increases the formation of calcified bone, thereby inhibiting osteoporosis.
  • the sclerotia chinensis mainly contains naringin, sclerotin, and sclerotium.
  • the deposition has a significant promoting effect, which improves the activity of alkaline phosphatase (ALP) in the tissue, promotes the synthesis of proteoglycans, and inhibits collagen synthesis.
  • ALP alkaline phosphatase
  • the experimental bone injury model of the lower femur of rats was used to show that the root decoction of the fern can promote the healing of experimental bone injury in rats, and the effect is also enhanced with the increase of dose.
  • Polygonatum mainly contains saponins, polysaccharides, amino acids and trace elements. Studies have shown that Polygonatum can enhance immune and anti-fatigue effects.
  • Angelica mainly contains volatile oil. Studies have shown that Angelica can promote hematopoietic function of bone marrow and spleen cells, and significantly increase hemoglobin and red blood cell count. Aqueous solution can promote the recovery of hematopoietic function of bone marrow and spleen cells.
  • Panax notoginseng mainly contains notoginsenoside, notoginseng, quercetin, volatile oil and so on. Studies have shown that Panax notoginseng can promote the proliferation and differentiation of rat osteoblasts and promote osteoblast OPG.
  • Casein phosphopeptide is a polypeptide extracted from natural casein. It can increase the solubility of calcium and promote the absorption of calcium. Studies have shown that CPP has a positive effect on the absorption and utilization of calcium in humans and animals. As a promoting factor, CPP is a very effective calcium absorption promoting factor and can improve the bioavailability of metal ions such as iron, zinc and magnesium. Currently used as a nutritional supplement in Japan, Europe and China, it is widely used in food, health care, and literary industries.
  • composition of the present invention have a combined calcium source, increase bone density, prevent and treat osteoporosis, relieve fatigue, and enhance immunity.
  • the composition of the present invention is suitable for a group of middle-aged and elderly people who are deficient in calcium and osteoporosis, as well as a frail adult population. Detailed ways
  • Example 1 Preparation method of vinegar egg powder
  • the citrus juice was cooled to 28 ° C, added to the activated yeast (Anji Yeast Co., Ltd.), and fermented at 28 ° C for three days, the alcohol content of the fermentation broth was 6-7% (v / v), total sugar
  • the temperature is reduced to below 1Og/1
  • the fermentation is stopped to obtain citrus fruit wine.
  • Add 3% cultured acetic acid bacteria (Shanghai Difa Brewing Biological Products Co., Ltd.), stirring speed is 150r/min, ventilation ratio is 1:0.8, fermentation at 32 °C for 4 days, to total acid (calculated as acetic acid) Stop fermentation when rising to 6-7%.
  • the mixture was sterilized by heating at 80 ° C for 15 minutes to obtain 1200 g of citrus fruit vinegar having an acetic acid content of 6-8%.
  • Example 2 Preparation method of vinegar egg powder
  • Example 3 lg/tablet tablets
  • Vinegar powder (vinegar powder prepared in Example 1) 150g
  • the thick paste is mixed with a formulated amount of vinegar egg powder, calcium carbonate, casein phosphopeptide, and a tablet-acceptable adjuvant, granulated, 0.3% magnesium stearate, and tableted.
  • Example 4 lg/granule capsule
  • Vinegar powder (vinegar powder prepared in Example 1) 150g
  • Casein Phosphopeptide (CPP) 30g The amount of Epimedium, Rhizoma Dry, Huang Jing, Angelica, and Sanqi, soaked in water for 0.5 hours, decocted twice, the first time adding water 8 times, and boiling for 1.5 hours; Add water 6 times for the second time and cook for 1 hour. After filtration, the filtrates were combined and concentrated to a clear paste (relative density of about 1.10 at 60 ° C). Add alcohol to the concentration of ethanol to 60%, let stand for 24 hours, filter, and recover the ethanol from the filtrate and concentrate to a thick paste (the relative density is about 1.30 at 60 °C).
  • the thick paste is mixed with a formulated amount of fruit vinegar powder, calcium carbonate, casein phosphopeptide, and a capsule acceptable auxiliary agent, granulated, and encapsulated.
  • Example 5 4 g/pack of granules Ingredients 1000 packs
  • Vinegar egg powder (vinegar powder prepared in Example 1) 300g
  • Casein Phosphopeptide (CPP) 60g The formula amount of Epimedium, Rhizoma Drynariae, Polygonatum, Angelica, Sanqi, soaked in water for 0.5 hours, decocted twice, the first time adding water 8 times, boiling for 1.5 hours; Add water 6 times for the second time and cook for 1 hour. After filtration, the filtrates were combined and concentrated to a clear paste (relative density of about 1.10 at 60 ° C). Add alcohol to the concentration of ethanol to 60%, let stand for 24 hours, filter, and recover the ethanol from the filtrate and concentrate to a thick paste (the relative density is about 1.30 at 60 °C). The thick paste is prepared by mixing and granulating the formulated amount of fruit vinegar powder, calcium carbonate, casein phosphopeptide, and an auxiliary agent acceptable for the granule.
  • Example 7 Safety Toxicology Test
  • Test substance A tablet produced by the formulation of Example 3 of the present invention is hereinafter referred to as a test substance.
  • Test animals (The following 2 and 3 experiments) ICR mice, SD rats were provided by the Zhejiang Experimental Animal Center, medical laboratory animal certificate No. 22001001, clean grade. (4 feeding experiment) SD rats were provided by Shanghai Experimental Animal Center of Chinese Academy of Sciences. The certificate number is No. 003 of Chinese Medicine, and the clean grade is 60-80g. 2 Acute toxicity test
  • TA touch, TA 102 set 5000, 1000, 200, 40, 8 g / dish five dose groups, each dose of parallel dish, repeat the test once.
  • a blank control group and a positive control group were set.
  • mice weigh 25-30g. The mice were randomly divided into three groups of 2.5, 5.0, 10.0 g/kg body weight, one negative control group (distilled water) and one positive control group (cyclophosphamide 60 mg/kg body weight), 10 in each group, 5 males and 5 females. The sample was administered twice at intervals of 24 hours, and the amount of gastric perfusion was 0.1 ml/10 g body weight (10.00 g/kg dose group was formulated into 5.00 g/kg and 0.2 ml/10 g was administered to the test substance).
  • mice were sacrificed 6 hours after the second gavage, bone marrow was taken from the sternum bone marrow, fixed in methanol, and stained with Giemsa. A total of 1000 polychromatic red blood cells (PCE) were counted per animal during the microscopic examination.
  • PCE polychromatic red blood cells
  • mice ICR male mice, weighing 26-34g. Three dose groups of 2.5, 5.0, 10.0 g/kg body weight, one negative control group (distilled water) and one positive control group (mizomycin C 2.0 mg/kg body weight) were set, with 7 rats in each group. Each group of samples was administered orally by O.lml/lOg body weight (5.00 mg/kg 5.00 g/kg to 0.2 ml/10 g to the test substance) for 5 consecutive days, and the control group was treated similarly. After the first 35 days after the first gavage, the mice were sacrificed by dislocation. Five mice were randomly selected to take the epididymis on both sides, and placed in a plate containing appropriate amount of normal saline.
  • the epididymis was cut with an ophthalmic scissors, and four layers of fluoroscopic paper were suction filtered. Direct smear, naturally dry, fixed in methanol, stained with 1% eosin. The sperm morphology was observed under high power microscope. Each mouse counted 1000 intact spermatozoa, the type and number of abnormal sperm were recorded, and the incidence of sperm abnormality (%) was calculated. The results are shown in Table 5.
  • SD rats were randomly divided into 4 groups, 20 in each group, half male and half female.
  • Test set to control group test substance 1.67, 3.33, 6.67g/kg body recombination, equivalent to 25 times, 50 times and 100 times the recommended amount of human (the recommended amount is 4.0g / day).
  • Each dose of the experiment was uniformly mixed into the basic feed according to the weight of 10%, and was fed by feeding means, free to eat and drink. The observation was continued for 30 days, and the body weight and food intake were recorded and the food utilization rate was calculated.
  • blood was taken from the tail vein for hematological examination, and blood was taken for decapitation to perform blood biochemical examination.
  • the organs were generally observed for each organ, and the liver, kidney, spleen, testis (ovary) were weighed and the body was counted. And take liver, kidney, spleen, stomach, intestine, testis (ovary) for histopathological examination.
  • the experimental results are shown in Table 6, Table 7, Table 8, Table 9, Table 10, Table 11, Table 12.
  • Control group 10 5.56 ⁇ 0.6 65.1 ⁇ 2.5 32.2 ⁇ 3.2 32.9 ⁇ 2.1 0.98 ⁇ 0.1
  • Control group 10 5.66 ⁇ 0.5 63.3 ⁇ 4.2 31 ⁇ 6 ⁇ 3 ⁇ 5 31.7 ⁇ 2.4 0.99 ⁇ 0.2
  • the composition of the present invention has an oral LD 5Q of more than 10.00 g/kg body weight for both male and female mice, and is practically non-toxic.
  • Ames test, mouse bone marrow micronucleus test, mouse sperm abnormality test results were negative.
  • the results of the 30-day feeding test in rats showed no symptoms of poisoning in the experimental animals, and no abnormal pathological changes were observed in the gross anatomy. There were no damaging effects on blood routine and biochemical indicators.
  • Example 8 Increased bone density test
  • the tablets produced according to the formula 3 of the present invention are hereinafter referred to as the test materials, and are provided by Hangzhou Xinfu Pharmaceutical Co., Ltd., and the recommended intake is 4 tablets per person per time, once a day.
  • Test animals 50 clean-ranked weaned milk with an initial body weight of 60-75 g WISTAR male rats, provided by the Institute of Laboratory Animals, Chinese Academy of Medical Sciences, license number: SCXK (Beijing) 2000-0006.
  • Rats were randomly divided into 5 groups, namely low calcium feed control group, low, medium and high dose test groups, which were equivalent to 5 times, 15 times and 30 times the recommended amount per kilogram of body weight for adults.
  • a semi-synthetic feed was prepared at a dose of 0.335g/kg BW, 1.005g/kg BW, 2.010g/kg BW, and the content of the test substance in the feed was 3.35g/kg, 10.05g, respectively. /kg, 20.10g/kg), and then set a high-dose calcium carbonate control group according to the calcium content of the high-dose test group feed.
  • 10 in each group single cage feeding, free eating, drinking deionized water, weighing weekly, record Food intake. The test period is 12 weeks.
  • the fourth week of the fourth week of the eighth week of the twelfth week of the low calcium control group 10 85.4 ⁇ 5.3 195.4 ⁇ 11.5 274.8 ⁇ 20.2 326.8 ⁇ 24.9 high dose calcium carbonate group 10 85 ⁇ 2 ⁇ 5.3 199.5 ⁇ 11.6 278.8 ⁇ 20 ⁇ 4 323.0 ⁇ 26.0
  • Low-dose experimental group 10 85.6 ⁇ 5.4 203.2 ⁇ 11.4 285.4 ⁇ 15 ⁇ 2 333 ⁇ 7 ⁇ 20 ⁇ 2
  • Medium dose experimental group 10 85.0 ⁇ 5.3 206.6 ⁇ 13.1 287.0 soil 28.8 339.8 ⁇ 33.6
  • High-dose experimental group 9 85.2 ⁇ 4.8 210.7 ⁇ 13.2 300.4 ⁇ 20.5 344.6 ⁇ 21.4
  • Table 14 Fecal length, weight and bone calcium content of rats in the test article (M ⁇ SD) Group femur length Femur weight Femur total Calcium content of femur calcium content only
  • High dose calcium carbonate group 10 0.261 ⁇ 0.008* 0.258 ⁇ 0.009*
  • Low dose experimental group 10 0.253 ⁇ 0.010 0.247 ⁇ 0.008 medium dose experimental group 10 0.261 ⁇ 0.010* 0.249 ⁇ 0.008 high dose experimental group 9 0.267 ⁇ 0.013* 0.255 ⁇ 0.004* 3 test conclusion
  • the femur weight of rats was significantly higher than that of the low calcium control group (P ⁇ 0.05) by administering 2.010 g/kg BW test substance; 0.335 g kgBW, 1.005 g/kg BW, 2.010 g/kg BW test substance were administered.
  • the femur calcium content in rats was significantly higher than that in the low-calcium control group (P ⁇ 0.05).
  • the administration of 1.005g/kg BW and 2.010g/kg BW test specimens significantly increased the femoral telecentric end bone density of rats.
  • the tablets produced according to the formula 3 of the present invention are hereinafter referred to as the test materials, and are provided by Hangzhou Xinfu Pharmaceutical Co., Ltd., and the recommended intake is 4 tablets per person per time, once a day, set low, medium,
  • the three high-dose groups were equivalent to 5, 10, and 30 times the actual intake of humans, and the negative control group (distilled water) was used to perform functional tests to relieve fatigue and enhance immunity.
  • the results are as follows:
  • NK cell activity of the high dose group was significantly different from that of the water control group (P ⁇ 0.05).
  • test substance has the function of enhancing immunity
  • the composition of the invention has no toxic and side effects, has a significant function of increasing bone density, and has the functions of relieving fatigue and enhancing immunity, and can be made to increase bone density, prevent and treat osteoporosis, relieve fatigue, and enhance immunity. Healthy food.

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Abstract

La présente invention a pour objet une poudre de vinaigre de fruit et d'œuf ainsi qu'un procédé d'élaboration de ladite poudre ou une préparation contenant une poudre de vinaigre et d’œuf, ainsi que ses applications. Ladite méthode comprend l'immersion d’œufs dans du vinaigre de fruit obtenu à partir d'orange ou d'aubépine dans un rapport compris entre 3:1 à 5:1, avant éminçage, passage sous filtre presse et séchage par atomisation. La préparation obtenue contenant de la poudre de vinaigre et d’œuf comprend la poudre de vinaigre et d'œuf obtenue par ledit procédé, du carbonate de calcium, de l'épimède, de la drynaire, du sceau de Salomon, de l'angélique, du notoginseng, et un phosphopeptide de caséine.
PCT/CN2006/000594 2005-04-01 2006-04-03 Poudre de vinaigre de fruit et d'œuf et préparation contenant une poudre de vinaigre et d'œuf WO2006108345A1 (fr)

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CNB2005100495325A CN100379359C (zh) 2005-04-01 2005-04-01 一种含醋蛋粉的组合物及用途
CN200510049532.5 2005-04-01

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CN103610066B (zh) * 2013-12-07 2016-07-06 李保来 一种保健品醋蛋粉及其制备方法
CN104544101B (zh) * 2015-02-06 2017-01-04 四川省宜宾市思坡醋业有限责任公司 一种晒醋醋蛋液及其制备方法
CN106418507A (zh) * 2016-09-29 2017-02-22 安徽科技学院 一种醋制鸡蛋、芹菜根和茼蒿根营养保健咀嚼片及其制备方法
CN106360419A (zh) * 2016-09-29 2017-02-01 安徽科技学院 一种醋制鸡蛋、黄豆和黑豆营养保健咀嚼片及其制备方法
CN106418274A (zh) * 2016-09-29 2017-02-22 安徽科技学院 一种醋制鸡蛋香菇营养保健咀嚼片及其制备方法
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