WO2005037986A1 - 培養細胞シート包装体 - Google Patents
培養細胞シート包装体 Download PDFInfo
- Publication number
- WO2005037986A1 WO2005037986A1 PCT/JP2004/015188 JP2004015188W WO2005037986A1 WO 2005037986 A1 WO2005037986 A1 WO 2005037986A1 JP 2004015188 W JP2004015188 W JP 2004015188W WO 2005037986 A1 WO2005037986 A1 WO 2005037986A1
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- WO
- WIPO (PCT)
- Prior art keywords
- cell sheet
- cultured cell
- tray
- package
- space
- Prior art date
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Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0697—Artificial constructs associating cells of different lineages, e.g. tissue equivalents
- C12N5/0698—Skin equivalents
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2502/00—Coculture with; Conditioned medium produced by
- C12N2502/09—Coculture with; Conditioned medium produced by epidermal cells, skin cells, oral mucosa cells
- C12N2502/094—Coculture with; Conditioned medium produced by epidermal cells, skin cells, oral mucosa cells keratinocytes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2502/00—Coculture with; Conditioned medium produced by
- C12N2502/13—Coculture with; Conditioned medium produced by connective tissue cells; generic mesenchyme cells, e.g. so-called "embryonic fibroblasts"
- C12N2502/1323—Adult fibroblasts
Definitions
- the present invention relates to a cultured cell sheet package.
- cultured tissues have been obtained by culturing cells in vitro (in vitro). Such a cultured tissue can be used as a transplant tissue for filling a defect in a patient or a test tissue for examining the efficacy of a drug or cosmetics.
- the technology for producing a “cultured cell sheet” that extracts part of the skin tissue of the patient or the donor as a tissue for transplantation and cultures it in a sheet has reached the stage of practical application. For example, in the case of skin, attempts have been made to treat nevus, ulcers, burns, tattoos, etc., and to transplant cultured epithelial (epidermal) cell sheets into the rubbed skin from which skin for transplantation has been collected.
- This cultured epithelial (epidermal) cell sheet is prepared using a tissue engineering technique, and cultured in a culture flask so that the epithelial (epidermal) cells have a thin sheet-like structure, and then detached from the culture flask and transplanted. It has been offered to.
- Japanese Patent No. 2733800 discloses that a cultured epidermal cell sheet clipped to a substrate sheet is placed on the bottom surface of a recessed portion of a tray, and a liquid medium is put into the recessed portion of the tray, and then a sheet-shaped lid is formed. With the tray sealed Is disclosed.
- Japanese Patent Application Laid-Open No. 2002-335950 discloses a cell culture sheet that is immersed in a liquid medium while being sandwiched between protective devices, and sealed with a tray and a sheet-shaped lip.
- Japanese Patent Application Laid-Open No. 2003-070460 discloses a method in which a sheet is immersed in a liquid medium while being suspended on a special backing substrate that supports a fragile cultured cell sheet, and sealed with a tray and sheet-like lids. It is shown.
- an object of the present invention is to provide a cultured cell sheet package that can be transported without damaging the cultured cell sheet and that allows the cultured cell sheet to be easily removed. I do.
- the present inventors have developed a cultured cell sheet accommodated in an accommodation area on a tray bottom surface.
- the outer periphery of the tray is provided with a swing suppressing means for preventing the swing, and an operation space for allowing an operation for removing the accommodated cultured cell sheet is provided in the tray.
- the inventors have found that the above problems can be solved, and have completed the present invention. That is, according to the present invention, the following means are provided.
- a cultured cell sheet package that includes a tray and a lid and forms a space in which the cultured cell sheet is packaged in a state where the cultured cell sheet is immersed in a liquid, and substantially coincides with a periphery of the cultured cell sheet. Or one or two or more of which are arranged so as to define an accommodation area of the cultured cell sheet having a contour bulging outward by a predetermined distance from the periphery in the tray and to suppress the oscillation of the cultured cell sheet.
- the swing suppressing means can define an accommodation area for the cultured cell sheet on the bottom surface of the tray.
- the “operation” means that a finger or an instrument (hereinafter, the instrument is referred to as a holding device) capable of grasping or holding (hereinafter, simply referred to as a grasping) the cultured cell sheet reaches the cultured cell sheet. To remove the cultured cell sheet.
- the package may include one or more downward operation spaces formed below the periphery of the culture cell sheet accommodated in the accommodation area and inside the accommodation area. Can be. Still further, at least one of the lower operation spaces may be configured to communicate with at least one of the side operation spaces outside the lower operation space. Further, the cultured cell sheet package of the present invention,
- a cultured cell sheet package comprising a tray and a lid, forming a space in which the cultured cell sheet is packaged while being immersed in a liquid
- An area for accommodating the cultured cell sheet having a contour substantially coincident with the periphery of the cultured cell sheet or bulging outward by a predetermined distance from the periphery is defined in the tray so that the oscillation of the cultured cell sheet can be suppressed.
- the storage area may be defined on the bottom surface of the tray. Further, in these packages, one or two or more concave portions and Z that are formed inside the outline of the storage area can be formed in the storage area in the tray so that the lower operation space can be formed. Alternatively, one or more convex portions formed inside the outline of the storage area can be provided.
- At least one of the rocking suppression means can be integrally formed with the lid and / or the tray. Further, at least one of the movement suppressing means may be a vertical wall portion or a bank standing upright on a part of the side surface of the tray or the bottom surface of the former ancestral tray. The movement suppressing means may be formed so as to face the tray bottom surface to the lid.
- At least one lower operation space communicating with the side operation space thus formed can be provided.
- the swing suppressing means may be a separate member detachably provided to the tray.
- at least one space for the side operation can be formed in the tray after the separation of the movement suppressing means. Also, this result form It is also possible to provide at least one lower operation space communicating with the side operation space to be formed.
- a cultured cell sheet package including a tray and a lid and forming a space in which the cultured cell sheet is packaged while being immersed in a liquid
- An inner tray may be provided in the tray, and the inner tray may include the above-mentioned swing suppressing means. Further, the inner tray may have an operation opening portion capable of forming the operation space.
- a cultured cell sheet package comprising a tray and a lid, which forms a space in which the cultured cell sheet is packaged in a state of being immersed in a liquid
- a bottom portion having an area for accommodating the cultured cell sheet having a contour substantially coincident with the periphery of the cultured cell sheet or bulging outward by a predetermined distance from the periphery; and Means for suppressing movement of one or more cultured cell sheets arranged so as to be able to suppress swinging, and the cultured cell sheet accommodated in the accommodation area where the movement suppressing means is not arranged
- a cell culture cell sheet package having an inner tray detachable from the tray, which has one or two or more operation opening parts opened in the contour of the accommodation area when the operation is permitted, is also provided. Is done.
- an operation space in which the cultured cell sheet can be operated is not necessarily formed in the tray, and at least when the inner tray is taken out of the tray, the operation space is opened via the operation open portion. As long as the cultured cell sheet can be operated by using the above method.
- At least one of the operation opening portions may extend into the accommodation area at the bottom of the inner tray. Further, in this package, in a state where the inner tray is fixed in the tray, outside the operation opening portion of the inner tray, between the contour of the accommodation area and the peripheral wall of the tray. It is a preferable mode to provide an operation space in which the cultured cell sheet accommodated in the accommodation area can be operated.
- the lid and / or the tray may be provided with means for suppressing the floating of the cultured cell sheet.
- FIG. 1 is a view showing one embodiment of the cultured cell sheet package of the present invention
- FIG. 1 (a) is a plan view
- FIG. 1 (b) is a cross-sectional view taken along the line lb-lb in FIG. 1 (a). It is.
- FIG. 2 is a view showing one embodiment of the cultured cell sheet package of the present invention
- FIG. 2 (a) is a plan view
- FIG. 2 (b) is a cross-sectional view taken along line lib-lib of FIG. 2 (a).
- FIG. 3 is a view showing one embodiment of the cultured cell sheet package of the present invention
- FIG. 3 (a) is a plan view
- FIG. 3 (b) is a cross-sectional view taken along the line Illb-Ilb in FIG. 3 (a).
- FIG. 4 is a view showing one embodiment of the cultured cell sheet package of the present invention
- FIG. 4 (a) is a plan view
- FIG. 4 (b) is a cross-sectional view taken along line IVb-IVb in FIG. 4 (a). It is.
- FIG. 5 is a view showing one embodiment of a cultured cell sheet package of the present invention
- FIG. 5 (a) is a plan view
- FIG. S (b) is a cross-sectional view taken along line Vb-Vb of FIG. 5 (a). It is.
- FIG. 6 shows a modified example of the movement suppressing means relating to the cultured cell sheet package of the present invention.
- FIG. 7 shows a modification of the means for preventing lifting of the packaged cell sheet of the present invention.
- FIG. 8 is a diagram showing another embodiment of the cultured cell sheet package of the present invention.
- FIG. 8 (a) is a schematic perspective view of a component, and FIG. ), It is a longitudinal cross-sectional view at the lower operation space forming portion when the inner tray and the tray are combined.
- the cultured cell sheet package of the present invention comprises a tray and a lid, and forms a space in which the cultured cell sheet is packaged in a state where the cultured cell sheet is immersed in a liquid.
- An accommodation area for the cultured cell sheet having a contour substantially matching the contour of the cell sheet or bulging outward by a predetermined distance is defined in the tray, and is arranged so as to suppress the oscillation of the cultured cell sheet.
- the cultured cell sheet package of the present invention (hereinafter, also simply referred to as a package), the cultured cell sheet is stored in the storage area defined by the movement suppressing means, and the cultured cell sheet is packaged and transported or stored. During this time, the occurrence of damage due to rocking of the cultured cell sheet is suppressed by the rocking suppressing means.
- the periphery of the cultured cell sheet can be easily manipulated from the side operation space and the Z or lower operation space, so that the cultured cell sheet can be easily taken out and cultured at the time of removal. Damage to the cell sheet is also avoided.
- the cultured cell sheet is supported, and the sheet form can be maintained.
- the cultured cell sheet can be prevented from swinging due to vibration during transportation or the like, and the cultured cell sheet can be prevented from being damaged, and the cultured cell sheet can be easily taken out. .
- an accommodation area for the cultured cell sheet is defined on the bottom surface of the tray to suppress the movement of the cultured cell sheet.
- the package include a package that is provided with one or more cultured cell sheet rocking suppression means that are arranged as possible. According to this package, the cultured cell sheet is accommodated and supported in the accommodation area on the tray bottom surface defined by the swing suppressing means, so that the sheet-like form can be maintained.
- the bottom having an accommodation area for the cultured cell sheet and the culturing along the contour of the accommodation area are described.
- One or more of the cultured cell sheet rocking suppression means arranged so as to be able to suppress the rocking of the cell sheet, and a portion where the rocking restraining means is not disposed and which is housed in the housing area.
- a package having an inner tray that is detachable in the tray and has one or two or more operation opening parts that are opened along the contour of the storage area so that the cultured cell sheet can be operated.
- 1 to 5 and 7 show some examples of the package of the first embodiment. It should be noted that the various forms and aspects of the package shown below are merely examples for explaining the invention, and the present invention is not limited to these examples.
- the “cultured cell sheet” does not use a scaffold (also referred to as a carrier) as a scaffold for cell growth.
- a scaffold also referred to as a carrier
- the “sheet shape” refers to a shape in which the vertical and horizontal dimensions are extremely large with respect to the thickness (height) of the cultured tissue, and the shape is not particularly limited. And various shapes such as polygonal shapes.
- the scaffold includes various shapes such as genole, sponge, film, mesh, non-woven fabric, and woven fabric. Any embodiment may be used as long as it is performed.
- biocompatible materials are not limited to collagen and fibrin, but include hyanolenoic acid, polyglycolic acid (PGA), polylactic acid (PLA), polylactic acid dalycol polymer (PLGA), and polyrotaxane. And well-known materials.
- the cultured cell sheet may be not only a sheet composed of a single cell type, but also a composite type composed of a plurality of cell types.
- the cells constituting the cultured cell sheet various cells depending on the purpose of use are selectively used.
- Cell types include, for example, epithelial (epidermal) cells, fibroblasts, chondrocytes, osteoblasts, myoblasts, cardiomyocytes, hepatocytes, Vesicles, nerve cells, or their precursor cells, as well as mesenchymal stem cells (MSCs) and embryonic stem cells (ESCs), and their sources can also be derived from skin, cornea, oral cavity, bone marrow, etc. Any material may be used as long as it has characteristics suitable for the purpose.
- MSCs mesenchymal stem cells
- ESCs embryonic stem cells
- cultured cell sheets include cultured skin (composite of epidermal cells and fibroblasts), cultured epidermis, cultured dermis, cultured mucosal epithelium, and cultured corneal epithelium.
- Such cultured cell sheets are applied to burns, wounds, pressure ulcers, abrasions, bruises such as skin ulcers or nevus, inflammations such as keratitis and conjunctivitis, chemical wounds, laser abrasion-deficient wounds, or Alternatively, it is applied to test research on the irritancy of the cornea and the like and the toxicity of the compound.
- the cultured cell sheet can be contained alone in the present package, but can also be contained in the present package in a state of being suspended, sandwiched, or included in a carrier.
- the carrier is formed so as to have an outer shape capable of suspending, sandwiching, or enclosing the cultured cell sheet, and is made of a material having good biological safety, and is structurally or materially hygroscopic. It is formed to have moisture permeability. Examples of the outer shape or structure of the carrier include various forms such as a sheet, a film, a woven fabric, a nonwoven fabric, and a sponge.
- Such carrier materials include biopolymers of collagen, chitin, chitosan, polylactic acid, and polydalicholate, or blends of two or more of these, as well as polyethylene, polypropylene, polyethylene terephthalate, and polyethylene.
- Various polymers such as acrylate, polymethacrylate, polycarbonate, polyester, polyvinyl alcohol, poly 2-hydroxyethyl methacrylate, polyacrylamide, poly N-butylpyrrolidone, and polymer blends of two or more of these are mentioned. it can.
- Specific examples of such carriers include sterilizing gauze, biomaterial coating materials such as pesquitin (registered trademark), and arginic acid such as soapsan (registered trademark).
- colloid dressing for hides such as Duoactive (registered trademark), polyurethane film dressing such as Bioclusive (registered trademark), sterilized Japanese paper, sterilized nonwoven fabric, flexible polymer materials (for example, silicone rubber) ), Collagen gel, fibrin gel, gelatin, agar medium and the like in the form of a sheet.
- various clamping members having high physical properties as described in JP-A-2002-335950 and backing materials as described in JP-A-2003-70460 are also included.
- the package of the present invention includes a tray and a lid. Combined with the trays and rails, it forms a packaging space. In this packaging space, the culture cell sheet is immersed in a liquid and accommodated therein.
- the material of the tray and the lid is not particularly limited as long as they are inert and liquid-tight with respect to the liquid to be filled or the cultured cell sheet to be contained. Examples of the material include polyethylene, polypropylene, polystyrene, polycarbonate, and polyvinyl chloride.
- synthetic resins such as polytetrafluoroethylene, inorganic materials such as hydroxyapatite ceramics, alumina ceramics, and glass, and metal materials such as aluminum can be used.
- the "lid” and the “tray” are preferably sealed with a force S that may be engaged in an air-permeable state like a culture dish or a culture plate.
- a sealing method various well-known methods such as bonding, screwing, fitting, and welding can be used.
- the lid only needs to be able to close the opening of the tray, but may be configured to be able to open and close a plurality of times. In addition, it can be opened and closed by turning one end as a base. 1 to 5 and 7 show some embodiments of the tray 20 and the lid 10 in the present package.
- the liquid in the present invention a preservation solution that does not retain the properties and morphology of the cells and does not cause rapid deterioration during transportation is selected.
- the preservative solution include a liquid medium containing nutrients necessary for maintaining cells.
- the cultured cell sheet package of the present invention is used not only during transportation. In addition, it is effective against vibrations generated during the culturing operation, and also functions as a culture device for cultured cell sheets. Therefore, the liquid does not only mean a preservation solution, but also includes a liquid medium for culture.
- the rocking suppression means is arranged along the periphery so as to define the accommodation area of the cultured cell sheet and to suppress the movement of the cultured cell sheet.
- the movement suppressing means is formed along a certain contour, and the inside of the contour is defined as an accommodation area of the cultured cell sheet.
- the movement of the cell sheet (movement in the planar direction of the package) is suppressed by appropriately contacting the sheet. Therefore, it is sufficient that the cultured cell sheet can be accommodated in the accommodation area, but if the accommodation area is too large, the function of the movement suppressing means cannot be sufficiently exhibited. For this reason, the accommodation area has a contour substantially corresponding to the peripheral edge of the cultured cell sheet or bulging a predetermined distance from the peripheral edge.
- the predetermined distance from the periphery of the cell culture sheet to the bulging contour is preferably 5% or less, more preferably 3% or less, and still more preferably 1% or less with respect to the total length of the cultured cell sheet.
- the storage area is defined by the tray, and when the package is composed of a tray and a lid as in the present embodiment, it is defined on the tray bottom surface.
- the inner tray is defined at the bottom of the inner tray.
- the cultured cell sheet is not only a collection of cultured cells but also a sheet provided with a scaffold (a scaffold at the time of culture). Hold by pinching etc. It may be packaged with a carrier. Therefore, the outline of the cultured cell sheet that defines the accommodation area includes the outline (including the outline of the carrier) in these various forms of the cultured cell sheet.
- FIGS. 1 to 5 and 8 show specific examples of various swing suppressing means 21, 31, 41, 61.
- the accommodation area 24 is formed by arranging the swing suppressing means 21, 31, 41, 61 along the contour of the accommodation area at the bottom of the tray 20 or the bottom 60 a of the inner tray 60. are doing.
- FIG. 6 shows various patterns of the relationship between the movement suppressing means and the cultured cell sheet (accommodation area).
- the swing suppressing means can be formed on the entire contour of the accommodation area within a range in which the oscillation of the cultured cell sheet in the accommodation area can be suppressed, or can be formed only on a part thereof. In the case where the swing suppressing means is formed only in a part of the contour, and when the accommodation area is rectangular or polygonal, the swing suppressing means can be formed in the sides except for the vicinity of these corners. By securing the corners of the accommodation area as side operation spaces, it is possible to secure an operable area with a larger area than securing the side operation space at a part of the side of the accommodation area. The work of removing the cell sheet can be facilitated.
- the corners of the cultured cell sheet are susceptible to damage due to rocking, and the corners of the cultured cell sheet can be damaged by providing rocking suppression means on the sides without providing rocking suppression means at those parts.
- the swing can be effectively suppressed while avoiding the problem.
- the swing suppressing means can be constituted by a single swing suppressing means, or can be operated by an aggregate of two or many swing suppressing means.
- the arrangement and the number of the swing suppressing means can be variously set depending on the arrangement site and the number of the swing suppressing means, and further, the mode of the swing suppressing means itself.
- the cultured cell sheet when the cultured cell sheet is rectangular, it is sufficient to provide at least four swing suppression means, at least one on each side (see FIG. 6 (a)). In the case of a shape, three may be provided at intervals of 120 ° from the center of the circle (see Fig. 6 (b)). Further, if the swing suppressing means is configured to be large, it can be held by two as shown in FIGS. 6 (c) and (d), and as shown in FIGS. 6 (e) and (f). It is also possible to provide a single rocking suppression means, which surrounds almost the entire circumference and has only one cutout.
- each swing suppressing means can be used in various modes without any particular limitation as long as the movement suppressing means can be brought into contact with the peripheral edge of the cultured cell sheet to suppress the swing thereof.
- the external shape of each swing suppressing means may be, for example, a columnar body (including a cylindrical body or an outer skin-like body) having various horizontal cross sections, a vertical wall, a bank, or the like.
- the embodiment illustrated in FIGS. 1, 2 and 4 is a skin-like body having a rectangular horizontal section (part of the peripheral wall of the tray), and the embodiment illustrated in FIGS. 3, 5 and 8 includes: It is a bank or wall.
- the contact portions of these rocking suppressing means are appropriately dispersed in order to reduce damage to the cultured cell sheet.
- the contact parts of each swing suppressing means can be formed so as to have a large number of convexities.
- the convex planar form can take various forms such as a dot form, a line form, and a lattice form.
- the formation portion of the swing suppression means for the tray and the lid can take various forms. For example, four J-like shapes can be exemplified.
- the The first mode of the formation portion of the swing suppression means is a tray peripheral wall, and the swing suppression means is a convex portion protruding inside the tray (see FIGS. 1, 2, and 4).
- the swing suppressing means can be constituted by the tray material by utilizing the side surface of the tray. Therefore, the swing can be effectively suppressed, and an operation space described later can be easily formed.
- a part of the side surface of the tray 20 is protruded inward to form the movement suppressing means 21.
- These rocking suppression means 21 are integrally formed on the peripheral wall of the tray with respect to the rail displacement. By being integrally molded, an easily handled package can be obtained.
- the second mode is the tray bottom surface
- the movement suppressing means is a column-shaped, vertical wall-shaped or bank-shaped convex portion standing on the tray bottom surface (see FIG. 3 for the vertical wall shape).
- the accommodation area can be easily configured by using the tray bottom surface.
- a vertical wall-like portion projecting vertically upward from the bottom surface of the tray 20 is formed on each of the four sides, and the vertical wall-like portion forms the swing suppressing means 31. ing.
- the third aspect of the formation portion of the movement suppressing means is a lid (rear surface), and the swing suppressing means is formed of various types such as a column, a vertical wall, a bank, and the like protruding from the rear of the lid toward the tray bottom surface. It is a convex portion (see Fig. 5 for the vertical wall shape).
- the swing suppressing means is separated from the tray in conjunction with the separation of the lid from the tray and the opening of the tray, and the separation facilitates opening of the peripheral side of the storage area.
- the part after separation of the means can be used as a space for lateral operation.
- the swing suppressing means 41 is formed on the rear surface of the lip 10 so as to face the tray 20 and to be arranged in a vertical wall shape with respect to the bottom surface of the tray 20.
- the oscillation suppressing means 41 is morphologically equivalent to or similar to the oscillation suppressing means 31 shown in FIG. In FIG.
- the rocking suppression means 41 having a vertical wall shape is formed on the lid 10, but, for example, a rocking suppression means such as a columnar body (as shown in FIGS. 1, 2 and 4).
- the rocking suppression means 21) can be formed on the lid 10. By doing so, it is possible to obtain a swing prevention mode equivalent or similar to the swing suppression means 21 shown in FIG. 1, FIG. 2, and FIG.
- the swing suppressing means 41 is formed integrally with the lid 10 by molding.
- a fourth aspect of the formation portion of the swing suppression means is a convex portion provided detachably with respect to the lid and the Z or the tray. These convex portions are provided so as to be detachable from the lid and z or the tray, and are not fixed to other parts of the package.
- Such a swing suppression unit can be applied to the swing suppression units of the first to third embodiments described above. That is, the swing suppression means is detachably attached to at least the side surface of the tray, the swing suppression means is detachably attached to the bottom surface of the tray, and further, is attached to the back surface of the lid. It is also possible to attach a movement suppressing means so as to be separable. In this way, the swing suppressing means can be configured with a high degree of freedom of design without being limited by the form and material of the trail.
- the present package can be configured using a general-purpose trail such as a conventional dish or plate without producing a special trail with an integrated swing suppression unit.
- the side of the storage area is opened in the part after the separation, and as a result, the part after the separation is laterally operated. It can be used as a working space.
- the swing suppressing hand is not limited to securing the space for the side operation. Steps can be formed.
- the rocking suppression means is formed at the position most suitable for suppressing the movement of the cultured cell sheet, while the region corresponding to the most suitable region for gripping and taking out the cultured cell sheet is defined as the space for lateral operation. can do.
- the rocking suppression means at the portion where the side operation space is to be formed can be finally separated from the tray bottom surface, so that such rocking suppression can be achieved. Optimization of the part and the operation part becomes possible.
- the swing suppressing means is inserted into the concave portion provided on the bottom surface of the tray, and the concave portion is also used or communicated with the lower operation space.
- both the side operation space and the lower operation space communicating with the side operation space can be formed at once by separating the rocking suppression means from the concave portion.
- the swing suppressing means can be separated from the tray and the Z or the lid as in the above-described fourth embodiment, or can be slidable (slidable) outward or inside the accommodation area. It may be slidable along the periphery of the storage area or may be foldable outside the storage area. In this way, when taking out the cultured cell sheet, an operation space for operating by contacting from the side or below the periphery of the cultured cell sheet can be easily formed or expanded. In particular, when all of the rocking suppression means are detachable or slidable or foldable as described above, the entire periphery of the cultured cell sheet can be easily opened.
- the slidable rocking suppression means includes, for example, a slide guide or a rail. It can be formed by providing it on the bottom surface of the tray or the like constituting the storage region, and by providing a locking portion or a fitting portion to the rocking suppression means.
- the sliding range of the rocking restraint means that can slide inward and outward of the storage area functions as a rocking restraint means for the cultured cell sheet to be stored, and at the time of removal, the peripheral edge of the cultured cell sheet. It is preferable that the distance be within a range that can secure the movement to the outside of the accommodation area to such an extent that an appropriate introduction portion into the side operation space or the lower operation space can be formed.
- the movement suppressing means that can slide in and out of the accommodation area can form an appropriate accommodation area according to the size of the cultured cell sheet to be accommodated or the like.
- the fact that the swing suppressing means is foldable outside the storage area means that, for example, the swing suppressing means is fixed to the tray bottom surface or the like constituting the storage area, and the fixed portion to the tray bottom face is used as a base when necessary. This corresponds to the case where the swing suppressing means bends outward. Note that it is not necessary that the rocking suppression means be bent to be substantially flush with the accommodation area in the folded state.
- Such a swing suppression means can be made foldable, for example, by forming substantially the entirety of a material having high flexibility, or can be folded at a portion fixed to the tray bottom surface or in the vicinity thereof as a fragile portion. It can be made foldable by providing a base.
- the swing suppressing means in the container of the present invention defines the outline of the accommodation region and suppresses the swing (movement) of the cultured cell sheet in the planar direction. Therefore, the height is not limited as long as it has a height capable of suppressing such shaking. Preferably, it has a height that extends. By providing such a sufficient height, it is possible to suppress the swinging of the cultured cell sheet in the plane direction within such a height range. Therefore, at the time of pitching that may occur during transportation, etc., Can be suppressed.
- the package of the present invention includes a space for side operation and a space for Z operation or lower operation.
- the space for side operation is a space formed at the outside of the accommodation area, at least when the cultured cell sheet is taken out, in contact with the periphery of the cultured cell sheet from the side to allow operation.
- the “F-side operation space” is a space that is operable by contact with the periphery of the cultured cell sheet from below, and is formed inside the housing area.
- the term “gripping tool” refers to any instrument used to take out the cultured cell sheet from the accommodation area of the tray, and includes a pin cent, a spatula, a knife, and the like, and preferably corresponds to tweezers. Therefore, these operation spaces are large enough to receive either a finger or various gripping tools.
- the side operation space is a space region formed over the open portion (outline portion of the storage region) where the movement suppressing means is not disposed in the storage region and the tray peripheral wall. Therefore, this space is defined by the tray peripheral wall and the tray bottom near the opening.
- the shape of the lateral operation space is not limited as long as it can be operated from the side with respect to the periphery of the cultured cell sheet.
- the tray bottom surface in the side operation space may be flat or may be formed in a concave shape. Further, when the swing iqi control means is fixedly arranged on the tray bottom surface, the side operation space is formed in a portion where the swing suppression means is not arranged, but the swing suppression means is not provided.
- the part after being separated from the rocking suppression means force tray bottom surface is secured as a side operation space.
- a space for lateral operation is formed at least when the cultured cell sheet is taken out.
- the side operation space may include one or two or more. As described above, when the accommodation area (cultured cell sheet) has a corner, the side operation space is preferably formed at at least one corner.
- FIGS. 1 to 5 various modes illustrated in FIGS. 1 to 5 can be adopted.
- FIGS. 1, 2 and 4 the vicinity of the four corners of the cultured cell sheet T and the outside of a part of each side are all secured as side operation spaces 22.
- FIG. 3 the vicinity of the four corners of the cultured cell sheet T and a part of the outside of the sides are secured as side operation spaces 22.
- FIG. 5 the outside of the entire periphery of the cultured cell sheet T is secured as the side operation space 22 by the separation of the rocking suppression means 41.
- a portion where the rocking suppression means 21 is not formed is secured as a space for lateral operation.
- the package may also include a lower operating space.
- the lower operation space By providing the lower operation space, the periphery of the cultured cell sheet can be easily operated from the lower operation space, so that the cultured cell sheet can be easily removed and the cultured cell sheet can be prevented from being damaged at the time of removal. .
- the provision of the side operation space and the lower operation space further facilitates removal of the cultured cell sheet.
- the lower operation space is a space below the peripheral edge of the cultured cell sheet accommodated in the accommodation area and is formed inside the accommodation area. However, they can be formed. Further, the lower operation space may include one or more.
- the site to be formed is not particularly limited, and may be the entire inside of the contour of the accommodation area or a part thereof.
- the lower operation space is preferably a location convenient for taking out the seat, and is preferably formed immediately inside at least one side operation space.
- the lower operation space is in communication with the side operation space immediately outside. Since the lower operation space and the side operation space are communicated, the most suitable operation for taking out the cultured cell sheet in this connected space can be performed, and the cultured cell sheet can be more easily taken out. be able to.
- the gripping tool inserted from the space for the side operation can be allowed to directly reach the space for the lower operation.
- the cultured cell sheet can be easily and reliably gripped. '
- the lower operation space can be formed by various methods, but adopts a configuration in which the tray bottom surface, which is the mounting surface of the cultured cell sheet in the accommodation area of the cultured cell sheet, has a concave portion and a Z or convex portion.
- the following two embodiments can be mentioned.
- one or more convex portions are formed on the bottom surface of the tray in the accommodation area, and the cultured cell sheet is supported on these convex portions, thereby forming at least the periphery of the cultured cell sheet.
- a predetermined space is secured below the peripheral edge. According to this aspect, even when the liquid cannot be completely removed, the culture cell sheet is in the lifted state, so that the operation can be performed in a state where the liquid is reliably separated from the liquid.
- FIG. 2 shows an example in which a single convex portion 23 is formed at the center of the accommodation area 24 to form a lower operation space 25, and FIG. An example in which the lower operation space 25 is formed by forming the convex portion 23 is shown.
- the cultured cell sheet T can maintain the sheet shape stably, and the periphery of the cultured cell sheet is similarly defined as the lower exchange space 25 below the entire periphery of the cultured cell support sheet T.
- the cultured cell sheet T can be gripped in various forms, and any of the 22 lateral replacement spaces can reach below the cultured cell sheet T, thereby facilitating the removal operation.
- the convex portion 23 shown in FIG. 2 since the lower surface of the cultured cell sheet T can be supported by a relatively large surface, the deformation of the cultured cell sheet T can be suppressed, and the culture can be stably performed together with the floating suppressing means described later. Cell sheet T can be stored.
- the contact area with the cultured cell sheet T which supports the cultured cell sheet T in a dispersed manner is reduced, so that the cultured cell sheet T Damage is suppressed.
- the presence of a region through which the liquid can flow below the culture packet sheet ⁇ allows nutrient components and the like contained in the culture medium and the storage liquid to be reliably supplied to the cultured cell sheet T.
- the dot convex portions 23 provided on the bottom surface of the tray 20 are arranged at regular intervals, and therefore, through the side operation space 22 and the lower operation space 25, Further, the gripping tool can reach below the central portion of the cultured cell sheet T through the space between the convex portions 23. By doing so, the lower part of the central part of the cultured cell sheet can be supported by the gripper, and a more stable or highly flexible removal operation can be performed.
- a dot-shaped convex part that forms a certain gap so that the gripper can be inserted is provided.
- a line-shaped convex portion can be provided.
- the lower operation space 25 can be gripped via these groove portions and the like. Ingredients It can also reach below the central part of the cultured cell sheet T.
- a groove-shaped portion or the like may be formed so as to communicate the opposing lower operation space 25.
- all of these convex portions 23 support the whole of the portion near the center of the cultured cell sheet ⁇ , but the shape of the convex portion is not limited to these, and the shape of the accommodation region is not limited to these. Protrusions that support only the periphery of the cultured cell sheet in the vicinity may be used.
- the second mode of the lower operation space is a mode in which a concave portion is formed in at least a part of a portion corresponding to the peripheral edge of the cultured cell sheet on the bottom surface of the tray in the accommodation area.
- a predetermined lower operation space is formed below the cultured cell sheet in the portion where the concave portion is formed.
- the lower operation space can be easily formed only at a desired portion.
- the concave portion can be easily formed into a concave shape (for example, a hemispherical concave surface) suitable for lifting the peripheral edge of the cultured cell sheet with a gripper, so that the removal operation is easy.
- the degree of freedom of the formation site is high.
- 3 and 5 show examples of the formation of the lower operation space of this embodiment.
- a rectangular portion 26 is formed on the bottom surface of the tray 20 inside one side operation space 22, and as a result, the concave portion 26 forms a lower operation space 25. .
- the operation space 25 communicates with the lateral operation space 22 outside the operation space 25.
- the quadrangular portion 26 forms a part of the side operation space 22. Therefore, it is easier to hold the cultured cell sheet.
- the movement suppressing means 41 By separating from the bottom surface of the tray 20, the part where the movement suppressing means 41 is not formed in advance and the part after separation (the entire periphery of the cultured cell sheet) are separated from the side operation space 22. To form That is, the separated portion of the swing suppressing means 41 constitutes the side operation space 22. By doing so, even if the rocking suppression means 41 is provided on the entire contour of the accommodation area 24, the part after the rocking suppression means 41 is separated can be laterally manipulated. Working space 22 can be provided. Further, in this example, the four-sided portion 26 forming the lower operation space 25 is expanded to the outside of the accommodation region 24, and at least a part of the swing suppression means 41 provided on the lid 10 ( In FIG.
- the formation area of the concave portion 26 in order to reach the gripping tool further to the center of the cultured cell sheet T, it is possible to extend the formation area of the concave portion 26 to the center of the accommodation area, and Alternatively, the area in which the concave portion 26 is formed may be enlarged along the contour of the accommodation area 24 or outside thereof to facilitate the gripping.
- the package of the present invention may include one or both of the side operation space and the lower operation space. By providing either of them, the cultured cell sheet can be grasped and easily taken out from the tray, and by providing both, the grasping and taking out can be further facilitated.
- FIG. 1 shows an example of a package having only a side operation space. In this package, the cultured cell sheet T is brought into contact with the side operation space 22 and a part of the periphery thereof can be grasped and taken out.
- FIG. 2 shows an example of a package having a side operation space and a lower operation space. In this example, From the side operation space 22, a gripping tool can be inserted into the lower operation space 25 to securely hold the cultured cell sheet.
- the rocking suppression means is arranged over the entire contour of the cultured cell sheet.
- the culture cell sheet or the culture sheet is moved to one side in the accommodation area ⁇ , and the space between the cultured cell sheet and the fluctuation suppression
- the cultured cell sheet can be gripped by inserting the gripping portion.
- the lid and / or the tray may be provided with a floating suppressing means for preventing the cultured cell sheet from floating on the liquid surface.
- a floating suppressing means for preventing the cultured cell sheet from floating on the liquid surface.
- the surface may be suppressed to a certain height within the containment area and not reach a certain height.
- the floating suppressing means 11 is arranged so as to be alternate with the convex part 23 formed on the bottom surface of the tray 20, and the convex part 23 and the floating suppressing means 11 sandwich the same part in the cultured cell sheet T. Instead, they touch different parts and pinch them. As a result, an excessive pressing force due to the pinching is prevented from being exerted on the cultured cell sheet.
- the ridge 10 is provided with a holding pin-shaped floating suppression means 52 for holding the cultured cell sheet T, or as shown in FIG.
- a floating suppression means 54 is provided, and further, as shown in FIG. 7 (c), a ridge part corresponding to the upper part of the storage area is formed as a floating suppression means 56 which projects to the tray side, and the rising of the cultured cell sheet is led. It can also be directly held down by a part of it.
- FIG. 8 shows a package according to a second embodiment of the present invention. Also in the package of the second embodiment, the cell culture sheet, the tray and the lid, the movement suppressing means (excluding the portion where the swing suppressing means is formed), and the like are as described above. Hereinafter, a specific configuration of the package of the second embodiment will be described.
- an inner tray containing a cultured cell sheet in the tray can be provided.
- the inner tray is formed so as to be fixable in a packaging space composed of a tray, a lip, and a force.
- the fixing position is not particularly limited, but a sufficient amount of liquid such as a medium filled in the tray is sufficient.
- the bottom of the inner tray is located at a height such that it is supplied to the inner tray.
- the inner tray can be detachable from the tray.
- various fixing means such as locking, screwing, fitting and the like can be used. These fixing means need only be operably provided between the inner tray and the tray or the lid.
- a flange may be formed at the opening edge of the inner tray, and the flange may be detachably fixed to or near the opening edge of the tray, or a convex portion projecting downward at the bottom of the inner tray may be formed.
- the convex portion and the concave portion provided on the bottom surface of the tray can be detachably fitted to each other, or the convex portion provided near the opening edge of the inner tray is formed on the rear surface of the lid. It can also be detachably locked or fitted to the device.
- FIG. 8 shows the ridge 10 and the tray 20 including the inner tray 60. The illustrated tray 60 is fixed by locking the vicinity of the flange opening edge to the tray 20 opening edge.
- the inner tray may be fixed in the tray in advance by, for example, integral molding or the like, and the fixing state may be easily released by any method to allow only removal. Further, as described later, if the cultured cell sheet can be easily removed from the inner tray without removing the inner tray from the tray, the inner cell tray can be fixed in a form not to be removed. That is, in a state where the inner tray is fixed in the tray, the cultured cells accommodated in the accommodation area outside the opening area for operation of the inner tray and between the contour of the accommodation area and the peripheral wall of the tray.
- the inner tray can be integrated with the tray by providing an operation space that can operate the sheet, and the inner tray can be fixed to the tray without considering the ease of removal. Can be.
- the inner tray can be formed of the same material as the lid tray. Preferably, in order to supply the medium and various media filled in the tray sufficiently to the inner tray, for example, a part of the inner tray can be made liquid-permeable.
- the inner tray includes a bottom having an accommodation area for accommodating the cultured cell sheet, a swing suppressing means formed on the bottom, and an operation opening portion.
- the overall shape and size may be any as long as they can be arranged in the tray when the packaging space is formed, and may be such that the inner tray swing suppression means is inscribed in the peripheral wall of the tray.
- the swing suppressing means in the package of the second embodiment is formed along the contour of the housing area at the bottom of the inner tray.
- a convex portion such as a column, a vertical wall, or a bank standing on the bottom of the inner tray can be used.
- it is erected on the periphery of the bottom of the inner tray, and is more preferably formed by integral molding.
- FIG. 8 (a) a plurality of vertical wall-shaped movement suppressing means 61 are set up along the periphery of the bottom 60a of the inner tray 60 (which substantially matches the contour of the storage area 24). The installed state is described.
- the portion of the contour of the storage area at the bottom of the inner tray where no swing suppression means is formed is an open area for operation that allows the peripheral edge of the cultured cell sheet stored in the storage area to be operated from the side. I have.
- FIG. 8 (a) shows a part of a plurality of operation opening parts 62 which are part of a pair of opposing parts, and the opening part is inside the outline of the bottom part 60a and extends into the accommodation area 24. The state that has been reached is described
- the open portion for operation can also simultaneously function as a liquid passage section for supplying a medium or the like filled in the tray into the inner tray.
- a liquid such as a culture medium
- at least a part of the bottom of the inner tray or the like may be made of a material having liquid permeability.
- the package of the present embodiment can include the side operation space.
- the space below the operation opening portion can function as the above-described lower operation space. That is, the package of the second embodiment can be provided with a lower operation space. According to this aspect, the cultured cell sheet can be more easily operated and taken out.
- the package 12 shown in FIG. 8 when the inner tray 60 is placed in the tray 20, the outer periphery of the storage area 24 and the peripheral wall of the tray 20 are located outside all the operation opening portions 62.
- a side operation space 22 is provided throughout.
- some of the operation opening portions include a side operation space 22 and a lower operation space 25.
- a lower operation space may be provided in the inner tray thus formed. That is, in the package of the first embodiment, the same convex portion as that formed for forming the lower operation space on the tray bottom surface so that the lower operation space can be formed also at the bottom of the inner tray.
- a space for lower operation can be formed in the inner tray.
- Providing a lower operation space also in the inner tray facilitates the operation of the cultured cell sheet in the inner tray. Therefore, the lower operation space and the various forms for forming the space (the formation of the convex portion and the Z or concave portion) which have already been described in the package of the first embodiment are directly applied to the second operation space.
- the present invention is applied to the inner tray of the package of the embodiment.
- the package of the second embodiment means similar to the levitation suppressing means that can be provided in the package of the first embodiment can be provided in the lid and the Z or inner tray.
- the inner package By providing multiple trays, multiple inner trays can be arranged in a stack or in a plane in a single tray, and the volume of packaging (in transportation, storage, storage, etc.) for cultured cell sheets can be reduced. Can be Therefore, the package of the second embodiment is suitable for mass transportation.
- Such a package of the present invention can accommodate various types of cultured cell sheets, and can be used for storage, transportation, culture, and the like with the package closed. Sealing and sealing of the package can be performed by a conventionally known method. The same applies to opening the package.
- the cultured cell sheet package of the present invention can be used in any form as long as it is used to form a state for accommodating the cultured cell sheet, and is used for transportation, culture, storage, and the like. Used for Cultured cell sheets must be prepared in a specialized facility and then not damaged when transported to medical or research institutions. According to the cultured cell sheet package of the present invention, the demand can be satisfied even in the case of transporting a detached cultured cell sheet that is easy to handle on site and the cultured cell sheet can be easily taken out. be able to.
- Example 1 a cultured epidermal cell sheet composed of epidermal cells was packaged as a cultured cell sheet by the package shown in FIG.
- DMEM Dulbecco's modified Idyl medium
- FBS ⁇ fetal serum
- peeling medium 40 O PU / mL Deisupaze medium
- C 0 2 incubator 3 7 ° C, 1 0 % C 0 2
- PBS phosphate buffer
- a sterile nonwoven fabric with a slightly smaller area than the total area of the cultured epidermis cell sheet is overlaid on the cultured epidermis cell sheet, and the outer periphery of the cultured epidermis cell sheet protruding from the sterile nonwoven fabric is folded back to form a cultured epidermis cell sheet And a sterilized nonwoven fabric were suspended.
- a small amount of liquid medium (transportation medium) is placed in a sterile tray (not shown), and a sterile non-woven fabric having an area approximately 2.5 times that of the suspended cultured epidermal cell sheet is spread in the sterile tray as a carrier for the cultured cell sheet.
- the cultured epidermal cell sheet suspended on the sterilized nonwoven fabric was placed and wrapped on a sterile nonwoven fabric having a large area in a sterilization tray to form a cultured cell sheet-encapsulated carrier that encapsulated the cultured epidermal cell sheet.
- the package 2 shown in FIG. 2 is formed from a lip 10 and a tray 20.
- the side surface of the tray 20 is provided with a plurality of pillar-shaped swing suppressing means 21 formed by projecting a part of the peripheral wall of the tray inward along the side of the roughly rectangular contour.
- a rectangular accommodation area 24 is formed by the inner surface of the suppression means 21.
- the corners of the rectangular accommodation area 24 where the swing suppression means 21 are not formed and a part of the sides are open to the outside.
- One side operation space 22 is formed.
- the central portion of the bottom surface of the tray 20 is convex upward and forms a convex portion 23 having a flat top surface.
- the convex portion 23 supports a portion near the center of the carrier T containing the cultured cell sheet (hereinafter, simply referred to as carrier ⁇ ). On the other hand, since the convex portion 23 is not formed at the peripheral portion of the accommodation region 24, when the carrier T is accommodated in the accommodation region 24, the peripheral edge of the carrier T is arranged to protrude outward from the convex portion 23. A predetermined lower operation space 25 is formed below the periphery of the tray 20 with the bottom surface of the tray 20.
- the carrier T is placed in the storage area 24 of such a tray 20.
- the rocking suppression means 21 is arranged so as to come into contact with a plurality of portions of the outer periphery of the carrier T, or to come in contact with the carrier T immediately after being slightly rocked.
- the portion where the rocking suppression means 21 does not exist was in contact with the peripheral side of the carrier T via the side operation space 22 and was operable.
- a lower operation space 25 is established below the entire periphery of the carrier T, and the side operation space 22 and Z or the lower operation space 25 is provided for the periphery of the carrier T. It was operable from the side and below the carrier T via the.
- the carrier T was restrained from oscillating in the package 2 and was housed in the housing area 24 while maintaining a sheet-like form. Further, when the package 10 is opened by peeling off the lid 10 and the carrier T is taken out, when taking out using the tweezers as a gripper, the side operation space 2 2 where the rocking suppression means 21 is not formed is used. Can be easily operated on the periphery of the carrier T, and a lower operation space 25 is formed below the periphery of the carrier T over the entire periphery, so that the gripper is used by inserting the gripper here. As a result, the carrier T was securely held and lifted, and could be easily taken out of the tray 20.
- the side operation space 22 is formed in a region that is more than half of the contour of the accommodation region 24, and the lower operation space 25 is formed below the entire peripheral edge of the carrier T.
- Example 2 is an example in which the same cultured epidermal cell sheet as in Example 1 was used as an example, and was packaged using a package as shown in FIG.
- the package 2 shown in FIG. 3 is formed of a rim 10, a tray 20, and a force.
- a plurality of vertical wall-shaped movement suppressing means 31 are provided on the bottom of the tray 20, and the arrangement is arranged.
- a rectangular housing area 24 is formed by the inner surface of the rocking suppression means 31 thus formed. As a result, the corners of the rectangular storage area 24 where the swing suppression means 31 are not formed and a part of the sides are open to the outside, and the gap between this open position and the side surface of the tray 20 is formed.
- a side operation space 22 is formed at the side of the vehicle.
- a lower operation space 25 can be formed.
- the carrier T is placed in the storage area 24 of such a tray 20.
- the oscillation suppressing means 31 is arranged so as to contact at least a part of the periphery of the carrier T or to come in contact with the carrier T as soon as it swings slightly.
- the portion where the movement suppressing means 31 does not exist was operable by contacting the peripheral side of the carrier T by the side operation space 22.
- a lower operation space 25 is established below a portion corresponding to the concave portion 26 of the carrier T, and in a portion where the lower operation space 25 is formed, the lower operation space 25 is formed with respect to the side and the lower side of the carrier. Operation was possible via the side operation space 22 and the lower operation space 25.
- the carrier T is prevented from swinging in the package 2 by the swing suppressing means 31, and the sheet T is maintained in the storage area 24 while maintaining the sheet shape.
- the swing suppressing means 31 is formed almost entirely except for the corners of the accommodation area 24 and the corners are opened, the carrier T is prevented from being damaged and stable. could be held. Also, peel off the lid 10 and open the package 2 to remove the carrier T. When removing the carrier using forceps, it is possible to easily contact the periphery of the carrier from the lateral operation space 22 where the movement suppressing means 31 is not formed, and to remove the lower part of the periphery of the carrier T.
- the carrier T can be securely gripped and lifted by using the gripper by inserting the gripper therein, and the carrier T can be easily taken out from the tray 20.
- the lower operation space 25 is a concave portion 26 having a semicircular cross section, the gripper can be easily inserted below the carrier T. Further, since the lower operation space 25 communicates with the side operation space 22 and is also a part of the operation space 22, the gripper can be smoothly inserted from the side of the carrier T downward. There was no damage on the removed carrier T, and there was no damage on the contained cultured cell sheet.
- Example 3 is an example in which the same cultured epidermal cell sheet as in Example 1 was used as an example and packaged using a package as shown in FIG. Note that the configuration including the preparation of the cultured epidermal cell sheet and the carrier enclosing the cultured cell sheet, which is common to the first embodiment, is omitted, and the description will focus on the differences from the first embodiment.
- the package 2 is formed with a lip 10 and a tray 20 and a force.On the inner surface of the tray 20, there are arranged a plurality of columnar bodies having a tray peripheral wall formed to protrude inward. A rectangular accommodating region 24 is formed along the inner side surface of the swing suppressing means 21.
- a part of the side of the rectangular accommodating area 24 where the movement suppressing means 21 is not formed is open to the outside, and the side operation space is provided between the open part and the side surface of the tray 20. 22 are formed.
- the planar shape is circular, and a plurality of convex portions 23 having a semicircular cross section are formed.
- the top of the convex portion 23 can support substantially the entire lower surface of the carrier T.
- these convex portions 23 are not formed on the bottom surface of the contour portion of the accommodation region 24.
- the ridge 10 has a large number of convex floating suppression means 50 which are directed to the bottom surface of the tray 20 at uniform intervals so as not to match with the convex portions 23 provided on the bottom surface of the tray 20. ing. The lower ends of these floating suppressing means 50 are formed so as to reach a position where the carrier T supported by the convex portion 23 can be held substantially at the supporting position.
- the clearance between the levitation suppressing means 50 and the protruding portion 23 is made substantially equal to the thickness of the carrier T, so that the device can be configured so as not to be affected by vertical vibration.
- the cultured epidermal cell sheet contained in the carrier T is held with a pressing force that does not damage the cultured epidermal cell sheet.
- the carrier T is placed in such a storage area 24 of the tray 20.
- the rocking suppression means 21 is arranged so as to come into contact with a plurality of portions of the outer periphery of the carrier T, or to come in contact with the carrier T as soon as it slightly moves.
- the portion where the rocking suppression means 21 does not exist was operable by contacting the peripheral side of the carrier T by the side operation space 22.
- a lower operation space 25 is established below the entire periphery of the carrier, and the periphery of the carrier T is provided via the side operation spaces 22 and Z or the lower operation space 25. Operable from the side and below the carrier T.
- the carrier T is prevented from swinging in the package 2 by the anti-swing means 21, and the sheet T is maintained in the storage area 24 while maintaining the sheet shape.
- the floating was suppressed by the floating suppressing means 50, the carrier was stably supported on the convex portion 23 of the tray 20, and the carrier T did not move up and down even during vertical swing.
- the package 10 is peeled off by removing the lid 10 and the carrier T is taken out, when taking out the carrier T using tweezers as a gripper, it is easy to remove the carrier T from the side operation space 22 where the movement suppressing means 21 is not formed.
- the carrier T can be operated around the periphery of the carrier T, and the lower operation space 25 is formed all around the periphery of the carrier T, so that the carrier T is securely inserted using the gripper by inserting the gripper here. It could be grasped and lifted, and easily taken out of the tray 20.
- the gripper can be smoothly inserted downward from the side of the carrier T.
- the convex portions 23 are formed at predetermined intervals so that the gripping tool can reach the center side of the carrier T, so that the gripping tool can be reached as far as necessary and taken out. Work could be done. There was no damage on the removed carrier T, and no damage was found on the contained cultured cell sheet.
- Example 4 is an example in which the same cultured epidermal cell sheet as in Example 1 was used as an example and packaged using a package as shown in FIG.
- the configuration including the preparation of the cultured epidermal cell sheet and the carrier enclosing the cultured cell sheet, which is common to Example 1, is omitted, and different points from Example 1 are mainly described. [Preparation of cultured cell sheet package]
- the package 12 shown in Fig. 8 is formed from the lip 10, the tray 20, the inner tray 60 and the force.
- a plurality of vertical wall-shaped swing suppression U means 61 are provided on the periphery of the bottom 60 a of the inner tray 60, and the inner IJ surface of the arranged swing suppression means 61 is arranged.
- a rectangular accommodating region 24 is formed.
- a plurality of portions of the rectangular accommodating region 24 where the rocking suppression means 61 is not formed are open to the outside, and these open portions are operating open portions 62.
- a plurality of flanges 66 projecting outward are provided on the upper end side of the plurality of swing suppressing means 61, respectively.
- the flange 66 is formed of a portion that protrudes substantially horizontally outward at a predetermined height of the swing suppressing means 61, and a portion that hangs downward from the protruding portion.
- Such an inner tray 60 is disposed approximately at the center of the tray 20, and the inner tray 60 is locked at a predetermined position on the tray 20 by locking the flange 66 of the inner tray 60 to the opening edge of the tray 20. That is, the bottom portion 60a of the inner tray 60 can be detachably fixed at a position where the bottom portion 60a is located at a predetermined height from the bottom surface of the tray 20.
- all the operation opening parts 62 are opened into the tray 20, and the bottom part 60 a of the inner tray 60 is outside the operation opening part 62.
- the side operation space 22 is formed outside the inner tray 60 and inside the tray 20. Further, in some of the operation opening portions 62, a lower operation space 25 is formed outside the inner tray 60 and inside the tray 20.
- the carrier T is placed in the storage area 24 of the inner tray 60.
- the swing suppressing means 61 is disposed so as to make contact with at least a part of the periphery of the carrier T, or to be in contact with the carrier T even immediately after swinging slightly.
- the portion where the movement suppressing means 61 is not present was in contact with the peripheral side of the carrier ⁇ ⁇ ⁇ by the operation opening portion 62 so that the operation can be performed.
- such an inner tray 60 is fixed to the tray 20.
- the periphery of the culture cell sheet T can be operated from the side even in the tray 20 by the side operation space 22 through the site 62.
- the periphery of the cultured cell sheet T can be operated from below also in the tray 20 by the lower operation space 25 via a part of the operation opening part 62.
- the carrier T is prevented from swaying in the package 2 by the swing suppressing means 61, and is maintained in the sheet-like form in the storage area 24.
- the swing suppressing means 61 is formed at predetermined intervals in the entire contour of the rectangular accommodating region 24, the carrier T can be stably held while suppressing damage.
- the carrier T can be reliably grasped and lifted by using the grasping tool by inserting the grasping tool into the open portion 62, and the carrier T can be easily removed from the tray 20. . Since a sufficient space is secured between the lower operation space 25 and the bottom surface of the tray 20, the gripper can be easily inserted below the carrier T. Further, since the lower operation space 25 communicates with the side operation space 22 and is also a part of the operation space 22, the gripper can be smoothly inserted downward from the side of the carrier T. Carried out There was no damage to a T, and there was no damage to the contained cultured cell sheet.
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Abstract
Description
Claims
Priority Applications (1)
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JP2005514778A JP4749155B2 (ja) | 2003-10-16 | 2004-10-07 | 培養細胞シート包装体 |
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JP2003-356683 | 2003-10-16 | ||
JP2003356683 | 2003-10-16 |
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Cited By (14)
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JP2007119033A (ja) * | 2005-10-31 | 2007-05-17 | Japan Tissue Engineering:Kk | 培養組織のための包装体及び培養組織包装体 |
JP2007269327A (ja) * | 2006-03-30 | 2007-10-18 | Japan Tissue Engineering:Kk | 培養組織の包装容器、これに使用するユニット、およびこれを使用した培養組織の包装体 |
JP2007267619A (ja) * | 2006-03-30 | 2007-10-18 | Japan Tissue Engineering:Kk | 培養組織用トレイおよびこれの操作ユニット |
JP2008541763A (ja) * | 2005-06-01 | 2008-11-27 | アイアールエム・リミテッド・ライアビリティ・カンパニー | 自動処理のための細胞培養フラスコ、システム及び方法 |
EP2561829A1 (en) * | 2011-08-26 | 2013-02-27 | Terumo Kabushiki Kaisha | Tool for transferring membranous tissue, kit and method for transferring membranous tissue |
JPWO2013137426A1 (ja) * | 2012-03-16 | 2015-08-03 | テルモ株式会社 | シート状構造物の輸送用液体組成物 |
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JP2008541763A (ja) * | 2005-06-01 | 2008-11-27 | アイアールエム・リミテッド・ライアビリティ・カンパニー | 自動処理のための細胞培養フラスコ、システム及び方法 |
JP2007119033A (ja) * | 2005-10-31 | 2007-05-17 | Japan Tissue Engineering:Kk | 培養組織のための包装体及び培養組織包装体 |
JP2007269327A (ja) * | 2006-03-30 | 2007-10-18 | Japan Tissue Engineering:Kk | 培養組織の包装容器、これに使用するユニット、およびこれを使用した培養組織の包装体 |
JP2007267619A (ja) * | 2006-03-30 | 2007-10-18 | Japan Tissue Engineering:Kk | 培養組織用トレイおよびこれの操作ユニット |
EP2561829A1 (en) * | 2011-08-26 | 2013-02-27 | Terumo Kabushiki Kaisha | Tool for transferring membranous tissue, kit and method for transferring membranous tissue |
US9253975B2 (en) | 2011-08-26 | 2016-02-09 | Terumo Kabushiki Kaisha | Tool for transferring membranous tissue, kit and method for transferring membranous tissue |
JPWO2013137426A1 (ja) * | 2012-03-16 | 2015-08-03 | テルモ株式会社 | シート状構造物の輸送用液体組成物 |
JP2018121568A (ja) * | 2017-01-31 | 2018-08-09 | 学校法人東京女子医科大学 | シート状生体組織の輸送用容器及びそれを用いた輸送方法 |
WO2019017463A1 (ja) * | 2017-07-20 | 2019-01-24 | テルモ株式会社 | 脆弱物保持デバイス |
JP2019019107A (ja) * | 2017-07-20 | 2019-02-07 | テルモ株式会社 | 液体保持空間を具備する脆弱物保持デバイス |
JP2019017342A (ja) * | 2017-07-20 | 2019-02-07 | テルモ株式会社 | 液体受容空間を具備する脆弱物保持デバイス |
JPWO2019017463A1 (ja) * | 2017-07-20 | 2020-07-09 | テルモ株式会社 | 脆弱物保持デバイス |
JP7039202B2 (ja) | 2017-07-20 | 2022-03-22 | テルモ株式会社 | 液体受容空間を具備する脆弱物保持デバイス |
JP7181200B2 (ja) | 2017-07-20 | 2022-11-30 | テルモ株式会社 | 脆弱物保持デバイス |
US20210127664A1 (en) * | 2018-07-10 | 2021-05-06 | Terumo Kabushiki Kaisha | Device for transporting graft |
US12004507B2 (en) * | 2018-07-10 | 2024-06-11 | Terumo Kabushiki Kaisha | Device for transporting graft |
JP2021020688A (ja) * | 2019-07-26 | 2021-02-18 | 東京食品機械株式会社 | 包装体およびその製造方法 |
JP7422500B2 (ja) | 2019-07-26 | 2024-01-26 | ムルチバック・ジャパン株式会社 | 包装体およびその製造方法 |
WO2023033135A1 (ja) * | 2021-09-06 | 2023-03-09 | 真一 高崎 | 微生物用培養器材、培地成分入り微生物用培養器材、及びこれを用いた微生物数計測法 |
Also Published As
Publication number | Publication date |
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JPWO2005037986A1 (ja) | 2006-12-28 |
JP4749155B2 (ja) | 2011-08-17 |
CN100580076C (zh) | 2010-01-13 |
CN1867663A (zh) | 2006-11-22 |
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