Classifications

• • C—CHEMISTRY; METALLURGY
  • C07—ORGANIC CHEMISTRY
  • C07D—HETEROCYCLIC COMPOUNDS
  • C07D487/00—Heterocyclic compounds containing nitrogen atoms as the only ring hetero atoms in the condensed system, not provided for by groups C07D451/00 - C07D477/00
  • C07D487/22—Heterocyclic compounds containing nitrogen atoms as the only ring hetero atoms in the condensed system, not provided for by groups C07D451/00 - C07D477/00 in which the condensed system contains four or more hetero rings
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
  • A61K41/00—Medicinal preparations obtained by treating materials with wave energy or particle radiation ; Therapies using these preparations
  • A61K41/0057—Photodynamic therapy with a photosensitizer, i.e. agent able to produce reactive oxygen species upon exposure to light or radiation, e.g. UV or visible light; photocleavage of nucleic acids with an agent
  • A61K41/0071—PDT with porphyrins having exactly 20 ring atoms, i.e. based on the non-expanded tetrapyrrolic ring system, e.g. bacteriochlorin, chlorin-e6, or phthalocyanines
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
  • A61P13/00—Drugs for disorders of the urinary system
  • A61P13/08—Drugs for disorders of the urinary system of the prostate
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
  • A61P17/00—Drugs for dermatological disorders
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
  • A61P17/00—Drugs for dermatological disorders
  • A61P17/02—Drugs for dermatological disorders for treating wounds, ulcers, burns, scars, keloids, or the like
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
  • A61P17/00—Drugs for dermatological disorders
  • A61P17/06—Antipsoriatics
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
  • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
  • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
  • A61P31/04—Antibacterial agents
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
  • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
  • A61P31/10—Antimycotics
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
  • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
  • A61P31/12—Antivirals
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
  • A61P35/00—Antineoplastic agents
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
  • A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
  • A61P7/00—Drugs for disorders of the blood or the extracellular fluid
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
  • A61P9/00—Drugs for disorders of the cardiovascular system
  • A61P9/10—Drugs for disorders of the cardiovascular system for treating ischaemic or atherosclerotic diseases, e.g. antianginal drugs, coronary vasodilators, drugs for myocardial infarction, retinopathy, cerebrovascula insufficiency, renal arteriosclerosis
• • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
  • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
  • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
  • Y02E50/00—Technologies for the production of fuel of non-fossil origin
  • Y02E50/10—Biofuels, e.g. bio-diesel
• • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
  • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
  • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
  • Y02E50/00—Technologies for the production of fuel of non-fossil origin
  • Y02E50/30—Fuel from waste, e.g. synthetic alcohol or diesel

Definitions

• the present invention refers to meso-substituted porphyrins of formula (I) hereinafter reported, the processes related to their preparation and use as photosensitising agents for therapeutic, diagnostic and photosterilisation uses. State of the art
• tetrapyrrolic macrocycles like porphyrins or other photosensitisers, are able to preferentially localise in neoplastic tissues and, once photo-activated by irradiation with visible light, they are capable of generating hyper-reactive derivatives of oxygen such as radicals and singlet-oxygen. Due to their high intrinsic reactivity, these species trigger irreversible oxidative cytotoxyc processes against cells and tissues, thus being highly cytotoxic after the localisation of the photosensitiser onto targets and irradiation. Thanks to their properties, porphyrins are used as photosensitizers for the treatment of tumours in the so-called "photodynamic therapy" (hereinafter referred to as PDT).
• PDT photodynamic therapy
• a typical PDT protocol is based on the administration of an appropriate dose of photosensitizer, followed by irradiation of the tissues wherein the tumour is localised by using light of appropriate wavelength, thus causing, by the so-called photodynamic effect, the preferential or selective elimination of the tumour mass. Due to the selective localisation in tumour tissues these photosensitizers can therefore be used not only for therapeutic purposes, but also as diagnostic agents as their high fluorescence quantum yield allow the direct visualisation of the tumour lesion.
• photosensitizers can also be used in the treatment and diagnosis of pre-tumour pathologies and for various hyperproliferative diseases like psoriasis, actinic keratosis, atheromas, endoarterial hyperplasia and prostate hyperplasia, as well as for microbial photoinactivation and therefore in the treatment of bacterial and micotic infections.
• Porphyrin derivatives bearing cationic groups have been previously described (Merchat et al. J. Photochem. Photobiol. 32, 153-157, 1996; Merchat et al. J. Photochem. Photobiol. 35, 149-157, 1996) and assessed for their photodynamic properties in the bacteria photoinactivation. These compounds bear trimethyl- anilinium groups or quaternary ammonium pyridinium groups in the meso- positions and therefore are endowed by a hydrophilic nature.
• Other photosensitisers such as phthalocyanines having hydrophilic and/or amphiphilic characteristics are known; for example, the International Applications No. WO 01/96343 and WO 02/090361, and in the US Patent No. 5,965,598, all in the name of the Applicant, disclose various evenly substituted hydrophilic phthalocyanines, as well as non centrosymmetrical phthalocyanines bearing cationic or protonable group on the macrocycle. Summary of the invention
• the Applicant has now found a novel series of photosensitizers having particularly advantageous properties compared to the known compounds. These novel compounds have shown optimum physical-chemical features for therapeutic applications, particularly in relation to their absorption in the region of the visible spectrum, high molar extinction coefficients, high quantum yield in singlet oxygen production, that is expressed by the photoinactivation of eukaryotic and prokaryotic cells.
• the photosensitizers described by this invention are able to produce singlet oxygen by using various light sources and wavelengths.
• they can be activated by visible red light radiation when the treatment of deep seated tumours on infections is required as well as by blue visible radiation or white light radiations when is preferable to treat by means of the photodynamic process more superficial lesions such as in the treatment of psoriasis, actinic keratoses, basal cell carcinomas and other cancerous and pre-cancerous lesions of the skin and mucosas.
• more superficial lesions such as in the treatment of psoriasis, actinic keratoses, basal cell carcinomas and other cancerous and pre-cancerous lesions of the skin and mucosas.
• Of particularly interest is the possibility to modulate the activity of these products by acting on the radiation wavelength used for the activation.
• porphyrins are able to absorb light in the red region and in the blue region of the visible spectrum to a different extent.
• the combination of the light source devised to these purposes and the differential absorption by the porphyrins, allows a unique possibility in order to precisely define the activity of these compounds while used as photosensitizers in the photodynamic treatment of the above mentioned pathologies.
• the present compounds are therefore suitable for the photodynamic treatment of pathologies characterised by cellular hyperproliferation and for the photodynamic treatment of infections caused by pathogenic micro-organisms, however can be also used as in vivo diagnostic agents due to the fluorescence released after excitation at various wavelength in the visible region of the light spectrum.
• these derivatives can be used as sterilising agents in the in vivo treatment such as the treatment of wounds as well as in vitro treatments such as the blood or blood derivatives sterilisation.
• Subject of the present invention are therefore the porphyrins of general formula (I)
• X is selected from the group consisting of O, S, CH 2 , COO, CH 2 CO, 0(CH 2 ) 2 O,
• Z is selected from between N and CH 2 N;
• Y is selected from aliphatic groups, linear or branched, saturated or unsaturated, having from 1 to 10 carbon atoms, and phenyl, possibly substituted, or Y forms with Z a saturated or unsaturated heterocycle, possibly substituted, comprising up to two heteroatoms selected from the group consisting of N, O and S;
• R and R 5 equal or different from each other, are selected from H and alkyl groups having from 1 to 3 carbon atoms, or they form with the Z group a saturated or unsaturated heterocycle, possibly substituted, comprising up to two heteroatoms selected from the group consisting of N, O and S;
• R 6 is selected from H and aliphatic groups, linear or branched, saturated or unsaturated, having from 1 to 5 carbon atoms, possibly substituted with alkylamine or alkylammonium groups having alkyl chains comprising from 1 to 5 carbon atoms, or forming a saturated heterocycle comprising up to two heteroatoms selected from between O and N; d, m, and n, equal of different from each other, are selected from 0 and 1 ; v and s, equal or different from each other, are integers comprised between 1 and 3;
• Ri is selected from H and a group of formula (III)
• G is selected from H and P- (CH 2 ) (W) f - J, wherein P is selected from the group consisting of O, CH 2 , CO 2) NHCONH and CONH;
• I is an integer comprised between 0 and 5;
• W is selected from the group consisting of O, CO 2 , CONH and NHCONH; f is selected from between 0 and 1 ; J is H or an alkyl group (CH 2 ) q -CH 3 , wherein q is an integer comprised between 0 and 20;
• R 2 and R 3 are selected from between R and
• M is chosen from 2H and a metal selected from the group consisting of Zn, Mg, Pt,
• R 7 is chosen from between H and C1- C15 alkyl, and pharmaceutically acceptable salts thereof.
• the present compounds of formula (I), as well as the corresponding conjugates, are useful for the treatment of microbial infections (bacterial, fungal and viral), in the photodynamic treatment of tumour, pre-cancerous pathologies, and other hyperproliferative diseases.
• the present compounds (I) and the corresponding conjugates are useful as well, as diagnostic agents for the identification of pathologically affected areas and for photodynamic sterilization of blood and blood derivatives.
• an heterocycle is preferably meant, which is selected from the group consisting of morpholine, piperidine, pyridine, pyrimidine, piperazine, pyrrolidine, pyrroline, imidazole, aniline and julolidine (2,3,6, 7-tetrahydro-1 H,5H pirido[ 3,2,1-//] quinoline).
• the present porphyrins of formula (I) have two or three groups with amino or ammonium substituents in two or three of the four meso-positions, and H or groups with non polar substituents on the other meso-positions.
• Preferred compounds according to the invention are those wherein the group R comprises substituents bearing tertiary or quaternary nitrogen; more preferred are the present compounds of formula (I) wherein the group is selected from the group consisting of:
• compositions of porphyrins of general formula (I) according to the invention bearing basic substituents, include conventional acid addition salts, obtained by the addition of HCI, H 3 PO 4 , H 2 SO , HBr or by ion-exchange. Additionally, salts obtained by reaction of a carboxylic function or acid groups with the porphyrin ring are within the scope of the present invention.
• the present compounds of formula (I) show valuable photodynamic characteristics making them useful in photodynamic therapy (PDT) against bacterial, fungal, and viral infections, for various hyperproliferative diseases, as well as for photosterilization of blood and blood derivatives, such as platelets and erythrocytes.
• the present compounds can be added to blood or blood derivatives as such or bound to suitable matrix, according to the known techniques, and thereafter irradiated. Moreover they can be used as diagnostic agents for the identification of pathologically affected areas.
• the present products possess a high molar absorption coefficient, which represents an important requirement for an effective therapeutic response.
• tissue penetrating radiation having a wavelength around 650 nm
• they are suitable for the use in PDT against diseases, both dermatological and internal, but they also can be activated by using less tissue penetrating light for the photodynamic treatment of very superficial lesion, especially when a little damage of the skin should be accomplished or, for instance, when a fluorescence emission from the tissue is preferred such as in the photodiagnostic procedures of the above mentioned pathologies.
• the products formed by photobleaching of the present compounds are non toxic. This finding reinforces their usefulness as therapeutic since, after having exploited their action, the compounds are inactivated by the light and then are no more potentially toxic in vivo.
• the present compounds are active in the singlet oxygen production or allow the production of reactive species of oxygen under conditions of poor oxygenation. Such requirement is particularly important because it allows to treat specifically anaerobic micro-organisms or tumour cells, well-known characterised by an environment poor of oxygen.
• the present compounds possess very high efficiency for microorganisms such as yeasts, fungi and mycoplasma, Gram-positive and Gram- negative bacteria, and show a great ability in specifically localising on microorganisms compared to the mammalian host cells.
• the present invention comprises also the above described formula (I) compounds site-specifically conjugated with a bio-organic carrier able to direct to a definite target.
• the carrier is usually chosen among molecules having well-known specific binding capacities, for example aminoacids (preferably basic aminoacids), polypeptides (preferably consisting of basic aminoacids), proteins and polysaccharides normally used for targeting purposes.
• the binding porphyrin/carrier may occur for example between the corresponding amino or carboxyl groups, or may occur involving other specific functional groups on the porphyrin moiety or on the carrier molecule.
• Functional groups such as thiol, maleimide derivatives, ⁇ -bromo esters and amides, diazonium salts and azido derivatives can be introduced according to known procedures in order to pre-functionalise both the porphyrin or the carrier depending upon the selected carrier itself and its stability.
• the compounds of the present invention can be prepared according to processes known in organic chemistry starting from suitable reagents.
• they can be prepared for example according to a process comprising the synthesis of the porphyrin ring through dipyrromethane followed by functionalisation of the porphyrin with aliphatic or aromatic amino or ammonium groups, and possible complexation with the metal cation if the metal complex is required.
• SCHEME 1A pre-functionalization of suitable reagents (selected so to form the porphyrin ring) with amino groups, followed by statistical synthesis of the porphyrin ring, and possible modification of the amino groups in ammonium groups
• SCHEME 1 B statistical synthesis with formation of the porphyrin ring followed by functionalization of the porphyrin with the present amino or ammonium groups
• the present porphyrins can be converted in the corresponding metal complexes by treatment with the suitable metal cation according to well-known procedures in organic chemistry.
• the introduction of the metal cation into the porphyrin ring may be achieved before or after the functionalisation of the porphyrin ring with the present amino or ammonium groups.
• Step b) Synthesis of 4-(N,N-dimethylaminoethoxy)benzaldehvde
• 4-hydroxybenzaldehyde 500 mg, 4 mmol
• K 2 CO 3 662 mg, 4.8 mmol
• N,N-dimethyl-2-metilsulphonilethylamine 775 mg, 4.4 mmol
• the reaction mixture was heated to reflux, under magnetic stirring, for 4 hours, then poured in water to obtain a suspension from which the solid was isolated by filtration.
• the crude product was purified by chromatography on silica gel (ethyl acetate), to give 379 mg of pure product of the title (yield 49%).
• Step f) Synthesis of 5.10.15-tris-r4-(2-N.N.N-trimethylammoniumethoxy)phenyl1- 20-r(4-decyloxy)phenyl1porphyrinate zinc (II) triiodide
• Step q Synthesis of 5,10,15-tris-f4-(2-N.N.N-trimethylammoniumethoxy)phenvn- 20-r(4-decyloxy)phenvnporphyrin triiodide
• Step b1 Synthesis of 2-r4-(di-7H-pyrrol-2-ylmethyl)phenoxy1ethanol
• TFA 114 mg, 1 mmol
• the reaction mixture was kept under magnetic stirring, at room temperature, for 1 hour, then ethyl acetate (200 ml) was added and the solution washed with NaHC0 3 saturated solution, then the organic phase was dried with Na 2 SO 4 .
• the crude product was purified by chromatography (Petroleum ether/Ethyl acetate 1/1 ).
• Step c1 Synthesis of 5.10.15-tris-f4-(2-hydroxyethoxy)phenvn-20-r(4- decyloxy)phenyllporphyrin
• Step b) Synthesis of 3-(3-hvdro ⁇ ypropoxy)benzaldehvde To a solution of 3-hydroxybenzaldehyde (366 mg, 3 mmol) in anhydrous DMF (7 ml), under nitrogen atmosphere, K 2 C0 3 (829 mg, 6 mmol), and, after 10 minutes, 3-bromo-1-propanol (500 mg, 3.6 mmol) were added. The mixture was heated to reflux for 2 hours, then water was added and the product was extracted with CH 2 CI 2 . The organic layers were washed with water and with NaCI saturated solution, dried on Na 2 SO .
• Step c) Synthesis 5,15-bis- 3-(3-hvdroxypropoxy)phenyllporphyrin
• 2-(1H-pyrrol-2-ylmethyl)-1H-pyrrole (236 mg, 1.6 mmol) and 3-(3- hydroxypropoxy)benzaldehyde (305 mg, 1.6 mmol) in dry CH 2 CI 2 (160 ml)
• trifluoroacetic acid 114 mg, 1 mmol
• Step d) Synthesis of 5,15-bis- 3-(3-methylsulphonylpropoxy) phenyllporphyrin
• 515-bis-[3-(3-hydroxypropoxy)phenyl]porphyrin (30 mg, 0.049 mmol) in anhydrous CH 2 CI , under nitrogen atmosphere, at 0°C
• triethylamine 29 mg mg, 0.29 mmol
• methansulphonylchloride 17.
• the mixture was kept under magnetic stirring at 0 °C for 3 hours, then washed with NaHCO 3 saturated solution and with water.
• the organic phase was dried under Na 2 S0 4 and the solvent removed by evaporation.
• the crude product was purified by chromatography on silica gel (CHCI 3 ), to obtain 17 mg of the pure title product (yield 45%).
• Step e Synthesis of 5,15-bis-r3-(3-N,N-dimethylaminopropoxy))phenvn porphyrin
• K 2 C0 3 14 mg, 0.11 mmol
• dimethylamine hydrochloride 8 mg, 0.11 mmol
• Step f) Synthesis of 5,15-bis-r3-(3-N,N,N-trimethylammonium) propoxyphenyllporphyrin diiodide
• iodomethane 110 mg, 0.78 mmol
• the reaction mixture was kept under magnetic stirring, at room temperature, for 24 hours, then diethyl ether was added slowly until a precipitate appeared.
• the suspension was filtered and the solid crystallized from MeOH/diethyl ether to give 26 mg of pure title product (yield 70%).
• EXAMPLE 33 5.15-bis-(4-(2-[3-(N,N,N-trimethylammonium henyll-2-oxoethylV10.20-bis-r 3- decyloxy)phenyll porphyrin diiodide ESI-MS: m/z 639.4 Th (C 86 H 98 N 6 0 4 ) 2+
• 515-bis-[3-(3-hydroxypropoxy)phenyl]porphyrin 100 mg, 0.16 mmol
• CHCI 3 /THF 1/1 10 ml
• zinc acetate 183 mg, 1 mmol
• the solid was filtered through celite and the liquid phase was dried on Na 2 S0 4 , then the solvent removed by evaporation to obtain 110 mg of title product (yield 98%) that was used in the following step without further purification.

Landscapes

• Health & Medical Sciences (AREA)
• Chemical & Material Sciences (AREA)
• Organic Chemistry (AREA)
• Life Sciences & Earth Sciences (AREA)
• Medicinal Chemistry (AREA)
• Pharmacology & Pharmacy (AREA)
• Animal Behavior & Ethology (AREA)
• General Health & Medical Sciences (AREA)
• Public Health (AREA)
• Veterinary Medicine (AREA)
• General Chemical & Material Sciences (AREA)
• Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
• Chemical Kinetics & Catalysis (AREA)
• Engineering & Computer Science (AREA)
• Bioinformatics & Cheminformatics (AREA)
• Oncology (AREA)
• Communicable Diseases (AREA)
• Dermatology (AREA)
• Epidemiology (AREA)
• Molecular Biology (AREA)
• Urology & Nephrology (AREA)
• Biochemistry (AREA)
• Vascular Medicine (AREA)
• Virology (AREA)
• Heart & Thoracic Surgery (AREA)
• Cardiology (AREA)
• Hematology (AREA)
• Diabetes (AREA)
• Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
• Nitrogen Condensed Heterocyclic Rings (AREA)
• Nitrogen And Oxygen Or Sulfur-Condensed Heterocyclic Ring Systems (AREA)
• Medicinal Preparation (AREA)
• Radiation-Therapy Devices (AREA)
• Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
• Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)

Priority Applications (10)

Applications Claiming Priority (2)

Related Child Applications (2)

Publications (2)

Family

ID=32104761

Family Applications (1)

Country Status (17)

Cited By (7)

Families Citing this family (36)

Citations (3)

Family Cites Families (8)

• 2002
  • 2002-10-21 IT IT000200A patent/ITFI20020200A1/it unknown
• 2003
  • 2003-10-21 CA CA2503223A patent/CA2503223C/en not_active Expired - Fee Related
  • 2003-10-21 CN CNA2003801017372A patent/CN1714091A/zh active Pending
  • 2003-10-21 US US10/532,278 patent/US7947674B2/en not_active Expired - Fee Related
  • 2003-10-21 AU AU2003278118A patent/AU2003278118B8/en not_active Ceased
  • 2003-10-21 DK DK03769429.6T patent/DK1558616T4/da active
  • 2003-10-21 SI SI200331212T patent/SI1558616T2/sl unknown
  • 2003-10-21 DE DE60319406T patent/DE60319406T3/de not_active Expired - Lifetime
  • 2003-10-21 JP JP2004544280A patent/JP4751068B2/ja not_active Expired - Fee Related
  • 2003-10-21 PT PT03769429T patent/PT1558616E/pt unknown
  • 2003-10-21 AT AT03769429T patent/ATE387450T1/de active
  • 2003-10-21 WO PCT/EP2003/011642 patent/WO2004035590A2/en not_active Ceased
  • 2003-10-21 ES ES03769429T patent/ES2301840T5/es not_active Expired - Lifetime
  • 2003-10-21 EP EP03769429A patent/EP1558616B2/en not_active Expired - Lifetime
• 2005
  • 2005-04-18 IL IL168104A patent/IL168104A0/en not_active IP Right Cessation
  • 2005-05-20 ZA ZA200504102A patent/ZA200504102B/en unknown
• 2008
  • 2008-05-26 CY CY20081100540T patent/CY1107963T1/el unknown
• 2011
  • 2011-01-14 US US13/007,345 patent/US8288528B2/en not_active Expired - Fee Related

Patent Citations (3)

Non-Patent Citations (4)

Also Published As

Similar Documents

Legal Events