Classifications

• • A—HUMAN NECESSITIES
  • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
  • A23K—FODDER
  • A23K50/00—Feeding-stuffs specially adapted for particular animals
  • A23K50/80—Feeding-stuffs specially adapted for particular animals for aquatic animals, e.g. fish, crustaceans or molluscs
• • A—HUMAN NECESSITIES
  • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
  • A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
  • A23J3/00—Working-up of proteins for foodstuffs
  • A23J3/14—Vegetable proteins
  • A23J3/16—Vegetable proteins from soybean
• • A—HUMAN NECESSITIES
  • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
  • A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
  • A23J3/00—Working-up of proteins for foodstuffs
  • A23J3/30—Working-up of proteins for foodstuffs by hydrolysis
  • A23J3/32—Working-up of proteins for foodstuffs by hydrolysis using chemical agents
  • A23J3/34—Working-up of proteins for foodstuffs by hydrolysis using chemical agents using enzymes
  • A23J3/346—Working-up of proteins for foodstuffs by hydrolysis using chemical agents using enzymes of vegetable proteins
• • A—HUMAN NECESSITIES
  • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
  • A23K—FODDER
  • A23K10/00—Animal feeding-stuffs
  • A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
  • A23K10/14—Pretreatment of feeding-stuffs with enzymes
• • A—HUMAN NECESSITIES
  • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
  • A23K—FODDER
  • A23K20/00—Accessory food factors for animal feeding-stuffs
  • A23K20/10—Organic substances
  • A23K20/142—Amino acids; Derivatives thereof
  • A23K20/147—Polymeric derivatives, e.g. peptides or proteins
• • A—HUMAN NECESSITIES
  • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
  • A23K—FODDER
  • A23K20/00—Accessory food factors for animal feeding-stuffs
  • A23K20/10—Organic substances
  • A23K20/153—Nucleic acids; Hydrolysis products or derivatives thereof
• • A—HUMAN NECESSITIES
  • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
  • A23K—FODDER
  • A23K20/00—Accessory food factors for animal feeding-stuffs
  • A23K20/10—Organic substances
  • A23K20/189—Enzymes
• • A—HUMAN NECESSITIES
  • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
  • A23K—FODDER
  • A23K40/00—Shaping or working-up of animal feeding-stuffs
  • A23K40/30—Shaping or working-up of animal feeding-stuffs by encapsulating; by coating
• • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
  • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
  • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
  • Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
  • Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
  • Y02A40/81—Aquaculture, e.g. of fish
  • Y02A40/818—Alternative feeds for fish, e.g. in aquacultures

Definitions

• the present invention provides a feed for larvae and larvae using a low-phytin plant protein hydrolyzate with reduced phytic acid.
• the present invention also relates to a method for producing a low-phytin vegetable protein hydrolyzate in which phytic acid is reduced. Background technology
• soybean raw materials (soybean meal, soymilk, soybean protein, etc.) have been used as protein raw materials for fish farming feed.
• Examples of the invention for removing or decomposing phytic acid include: (a) feed (JP-A-11-000164, JP-A-8-205785); and (b) soybean-derived feed such as defatted soybean and okara. Materials (Japanese Patent Application Laid-Open No. Hei 9-140334), (c) soy protein (Japanese Patent Application Laid-Open No. 2000-300185, Japanese Patent Application Laid-Open No. 4503002), and (d) soymilk (Japanese Patent Application Laid-open No. No. 166049, Japanese Patent Application Laid-Open No. 2000-245340 by the present applicant) and the like.
• the present applicant has studied the use of a protein hydrolyzate obtained by hydrolyzing a soybean protein raw material using an enzyme as a feed for fish farming.
• the invention described in JP-A-7-227223 and the invention described in JP-A-8-51937 have been made as baits for increasing the survival rate of larvae and fry.
• soybean protein hydrolyzate obtained by removing phytic acid using a resin for patients with renal disease (Japanese Patent Application Laid-Open No. Hei 8-09921).
• a protease is used in the same way as a protease because a crude enzyme is used when enzymatically treating a soybean protein raw material, or a combination of enzyme digestion and phytase treatment is used. It does not disclose or teach the use of degraded soy protein for fish farming (Japanese Patent Application Laid-Open Nos. 51-125300, 2002-51706, etc.).
• soybean protein hydrolyzate As described above, the use of soybean protein hydrolyzate in fish feed has been studied by the present applicant, and low phytin soy protein hydrolyzate has also been studied. It is not known to be used for larvae or fry.
• the method for removing phytic acid from soybeans is as follows: (1) A method for removing phytic acid during the aqueous extraction of soybean protein using a salt (JP-A-8-173052, JP-A-9-1212) 780), (2) a method of decomposing phytic acid with phytase or the like, and (3) a method of removing phytic acid by adsorbing it on a resin or the like (Japanese Patent Application Laid-Open No. 2001-163800).
• the method (2) is industrially advantageous because the process is less complicated than the methods (3) and (3).
• Japanese Patent Application Laid-Open No. 9-023822 discloses that isolated soybean protein is inoculated with a bacterium, fermented, and simultaneously subjected to enzymatic decomposition and phytase treatment to obtain a peptide product having a low phytic acid content. A method is disclosed.
• an object of the present invention was to obtain a low-phytin plant protein hydrolyzate having a very low phytic acid content, and more preferably a low-phytin plant protein hydrolyzate having a phytic acid content below the detection limit.
• the present invention aims at a feed for fry using such a low-phytin plant protein hydrolyzate.
• the present inventors have conducted intensive studies and found that soy protein hydrolyzate used in feed By reducing the amount of phytic acid contained in the digest, the survival rate of the larvae and larvae was increased, and the present inventors obtained the knowledge of promoting the growth of the larvae and completed the present invention.
• a low-phytin plant protein hydrolyzate was completed. That is, the present applicant has previously completed a low-phytic acid plant protein hydrolyzate (JP-A-8-092123) by resin treatment, but in order to reduce the phytic acid content below the detection limit, the resin treatment was carried out. Was complicated.
• the present applicant produced a low phytic acid soybean protein by subjecting soybean protein to phytase treatment (Japanese Unexamined Patent Publication No. 2000-300185). It was difficult to obtain a soybean protein hydrolyzate with a very low phytic acid content below the detection limit.
• soybean protein hydrolyzate with extremely low phytic acid (below the detection limit) was obtained by first enzymatically decomposing soybean protein to a specific molecular weight range and then subjecting it to lyase treatment.
• phytic acid can be detected even if it is first treated with lyase and then enzymatically degraded. We have obtained information that can be removed below the limit.
• soybean protein hydrolyzate having a very low phytic acid content below the detection limit has a better flavor than the soybean protein hydrolyzate obtained by simply enzymatic degradation.
• the present invention has been completed based on these findings.
• the present invention is a feed for larvae and juveniles, wherein the feed material contains a low-phytin plant protein hydrolyzate having a phytic acid content of 0.05% by weight or less (in dry solid content). .
• a plant protein hydrolyzate having an average molecular weight of 200 to 10,000 is suitable.
• the present invention also provides a low phytin characterized by comprising (a) a step of degrading a protein using a protease and (b) a step of decomposing phytic acid by using an enzyme that degrades phytic acid. This is a method for producing a plant protein hydrolyzate. After decomposing the protein using a protease, it is preferable to decompose phytic acid using an enzyme that decomposes phytic acid.
• the enzyme that degrades phytic acid is preferably phytase.
• the pH for phytase treatment is preferably 6-9.
• the average molecular weight of the low phytin plant protein hydrolyzate is preferably from 200 to 100,000.
• the content of phytic acid in the low-phytin vegetable protein hydrolyzate is such that phytic acid is not detected by vanadomolybdate spectrophotometry (detection limit: 5 mg / lOOg) per dry solid.
• the low phytin plant protein hydrolyzate used in the feed for larvae and larvae of the present invention has a phytic acid content of 0.05% by weight or less, preferably 0.01% by weight or less, more preferably 0.01% by weight or less in dry solids. 0 4% by weight or less (below the detection limit) is appropriate.
• the average molecular weight of the low phytin plant protein hydrolyzate used in the larvae and feeds of the present invention is 200 to 100,000 (preferably 300 to 5,000). Things are appropriate. If the molecular weight is large, the effect of promoting the growth and survival rate of larvae and larvae is inferior, and if the molecular weight is reduced to amino acid, the osmotic pressure of the feed increases, and it becomes easy to dissolve, etc. Disappears.
• a soybean protein hydrolyzate with a relatively large molecular weight may be used for fish farming feeds alone, but it is preferable that the average molecular weight be smaller as the fish farming fish is a larva, especially a larva that has just hatched from an egg. Oligopeptide mixtures are preferred.
• the feed of the present invention suitably contains 40 to 70% by weight, preferably about 50 to 60% by weight of a protein component as a composition.
• the feed of the present invention contains 1% to 30% by weight, preferably 3 to 25% by weight of a low phytin plant protein hydrolyzate.
• a low phytin plant protein hydrolyzate usually, 3% by weight or more, preferably 10% to 80% by weight, of the protein component in the feed of the present invention is suitably replaced with the low-phytin plant protein hydrolyzate described above.
• the replacement ratio increases, it becomes difficult to granulate the feed.
• the amount of low-phytin plant protein hydrolyzate in the feed of the present invention is low, the effect of increasing the survival rate of larvae and juveniles and the growth rate of juveniles is small. This is common to fish such as red sea bream and inoculated fish that are difficult to produce for aquaculture, and is different from other fish farming.
• the protein components other than the low-phytin vegetable protein hydrolyzate include krill, fish meal, processed egg, processed milk, gelatin, fish meal, seafood extract, yeast extract, and fish egg extract. Can be.
• the feed of the present invention contains carbohydrates, fats, vitamins, minerals, n-3 highly unsaturated fatty acids, and phospholipids such as soybean lecithin, in addition to the aforementioned low-phytin vegetable protein hydrolysates and other proteins. Can be contained.
• n-3 polyunsaturated fatty acids are essential for saltwater fish
• feed-based phospholipids such as soy lecithin can be included in the feed of the present invention since they are necessary components for larval and young fish culture.
• the form of the feed of the present invention is preferably such that it has a particle size, buoyancy, sedimentation rate that is easy for fish to ingest, does not elute nutrients in water, and is digested and absorbed in the gastrointestinal tract. For larvae, it is preferable to prepare a fine particle feed by microencapsulation or the like.
• a method for encapsulating a low-phytin plant protein hydrolyzate having an average molecular weight of 200 to 100,000 by micro-mouth for example, a method of spray-drying an aqueous solution of the hydrolyzate can be adopted.
• fats and oils can be added before spraying to adjust the dissolution, floating and dispersibility in seawater, if necessary. Alternatively, it can be prepared.
• the feed of the present invention can be used in combination with a biological feed alone or in an appropriate amount at intervals of 30 minutes to 1 hour during the larval stage depending on the age of the larva.
• soy protein is one of the preferred protein raw materials since soy protein is industrially produced in large quantities. It is.
• the soybean protein raw material used for producing the low-phytin vegetable protein hydrolyzate of the present invention is a soybean protein such as soymilk (including skim soybean milk; the same applies hereinafter), concentrated soymilk, concentrated soybean protein, isolated soybean protein, defatted soybean, and the like. It is possible if it includes. Defatted soybeans are processed with little or no protein denaturation So-called low-denatured defatted soybeans that have been treated are preferred, and are not limited to varieties, production areas, and the like.
• defatted soybeans which have been subjected to low-temperature extraction using n-hexane as an extraction solvent are suitable as raw materials, and particularly low denaturing with an NSI (nitrogen solubility coefficient) of 60 or more, preferably 80 or more. Defatted soy is preferred.
• NSI nitrogen solubility coefficient
• Enzymatic degradation of soy protein can be obtained by hydrolyzing soy protein in an aqueous system (soy protein slurry or solution) using an enzyme.
• the concentration of the soybean protein solution to be subjected to the enzyme treatment is 1% by weight to 30% by weight, preferably 5 to 15% by weight, and more preferably 8 to 12% by weight. Appropriate. Even if the concentration is low, there is no problem in enzymatic decomposition, but productivity is unfavorably reduced.
• the proteolytic enzyme (protease) used in the present invention may be exoprotease or endprotease alone or in combination, and may be of animal, plant or microbial origin. Specifically, serine proteases (trypsin, chymotoribsin derived from animals, subtilisin derived from microorganisms, lipoxypeptidase, etc.), thiol proteases (papine, ficin, bromelain, etc. derived from plants) And carboxyprotease (such as animal-derived pepsin) can be used.
• serine proteases trypsin, chymotoribsin derived from animals, subtilisin derived from microorganisms, lipoxypeptidase, etc.
• thiol proteases papine, ficin, bromelain, etc. derived from plants
• carboxyprotease such as animal-derived pepsin
• Protin FN derived from Aspergillus oryzae (manufactured by Daiwa Kasei Co., Ltd.); “Alcalase” (manufactured by NOPO) and “Protin A” (manufactured by Daiwa Kasei Co., Ltd.) derived from Bacillus subtilis.
• endoproteinase-containing enzymes include “Protease S j” and “Protin AC-10” manufactured by Amano Pharmaceutical Co., Ltd., and “Protin AC-10” Biopase (Nagase Seikagaku Corporation) ) And Amino Pharmaceutical Co., Ltd., as a proteolytic enzyme containing exo and endoprotease. Ze "can be exemplified.
• hydrolysis conditions of the present invention vary somewhat depending on the type of protease used, but generally, hydrolysis of soybean protein is performed in the pH range, the temperature range, and the optimal reaction time of the protease. It is preferred to use a sufficient amount.
• a salt-restricted diet for example, a tube feeding diet
• the pH is 5 to 10, preferably 6 to 9, the salt by neutralization is used. It is preferable because the generation of can be reduced.
• the degree of hydrolysis is suitably an average molecular weight of from 200 to 100,000, preferably from 300 to 500,000.
• the degree of hydrolysis can be adjusted depending on the purpose and use. For example, in the case of feed or feed, there is no problem even with a relatively large molecular weight, but when used as a feed for fish farming, when used as a feed for larvae and larvae, a low molecular weight that is easy to digest is preferable, and the average molecular weight is It is suitably from 200 to 500, more preferably about 200 to 2000. (Phytic acid degradation by phytic acid degrading enzyme)
• Examples of the enzyme for decomposing phytic acid used in the present invention include enzymes derived from plants such as wheat and potato or enzymes derived from animal organs such as intestinal tract, bacteria, yeast, mold, and enzymes derived from microorganisms such as actinomycetes. Enzymes such as phytase-phosphatase having phytic acid decomposition activity can be used.
• phytophosphatase is suitable, but phytase is more preferable.
• Fischerichia can be used from various strains having a phytase-producing ability, such as Aspergillus, Rhizopus, Saccharomyces, Mucor, and Geotricum.
• it is derived from the genus Aspergillus, more preferably the genus Aspergillus: a phytase derived from Aspergillus ficuum and a phytase derived from Aspergillus niger.
• Ze and Aspergillus Terreus The phytase from (Aspergillus terreus) can be selected from just the group. In order to decompose phytic acid in soybean into inositol, it is necessary to cleave the ester group, and the enzyme that does this is phytase.
• a fungal acid phosphatase as the acid phosphatase. That is, it is selected from the group consisting of acid phosphatase derived from Aspergillus ficuum, acid phosphatase derived from Aspergillus niger, and acid phosphatase derived from Aspergillus terreus. I can do it.
• the degradation of phytic acid by enzymatic treatment can be carried out under very mild conditions, and therefore has very little effect on proteins.
• the enzyme reaction of the present invention may be performed at 30 to 60 ° C. for 0.1 to 30 hours.
• the pH during the phytic acid decomposition reaction is particularly important, and the pH is preferably 6 to 9, preferably 6.2 to 8.5, more preferably 6.2 to 7 pH. Soy protein treated at a pH of less than 6.0 is not preferred because its solubility is reduced and the flavor becomes worse. Further, if the pH exceeds 9.0, the flavor deteriorates, which is not preferable.
• decomposing phytic acid within the above pH range it is possible to produce a soybean protein with better reduced phytic acid.
• the enzyme suitably used in the present invention is preferably an enzyme capable of decomposing phytic acid and phytate in a neutral to alkaline pH range of pH 6 or more, but the origin is not particularly limited and the above-mentioned enzymes are not limited. You can use enzymes.
• the enzyme can be used irrespective of the form of powder or liquid.
• the enzyme is used in an amount of about 0.01 to 10% by weight, preferably about 0.05 to 2% by weight, more preferably about 0.1 to 1% by weight based on the weight of the crude protein in the soybean protein.
• the enzyme titer is 0.1 to 100 U / g crude soy protein, preferably 0.5 to 20 U / g crude soy protein, more preferably Preferably, phytase of about l to 10 U / g crude soybean protein is added.
• the enzyme activity was determined by reacting a reaction mixture consisting of 0.4 ml of 0.2 M Tris-HCl buffer containing 4 mM sodium phytate (pH 6.5), 0.5 ml of distilled water, and 0.1 ml of enzyme solution at 37 ° C for 30 minutes. After the reaction, add 10% TCA 1. Oml to stop the reaction. The inorganic phosphoric acid content in this reaction solution was determined by the Fiske-Subbarow method. The amount of enzyme that releases 1 ⁇ 1 of inorganic phosphoric acid per minute under the above conditions was defined as 1 unit (U).
• the order may be combined in any manner.
• phytin can be reduced, that is, the phytic acid content in the plant protein hydrolyzate can be reduced to 0.5% or less and 0.2% or less per dry solid content.
• the average molecular weight of the low phytin plant protein hydrolyzate obtained as described above is preferably from 200 to 100,000, and more preferably from 300 to 500,000.
• the phytic acid content was determined by the vanadomolybdate spectrophotometry (detection limit: 5 mgZl 0 g) in the dry solid content of the low phytin vegetable protein hydrolyzate. 5% or less, preferably one in which phytic acid is not detected.
• Water is added 7 times to 10 parts by weight of defatted soybeans, and extracted while stirring at 50 ° C and pH 7 for 30 minutes.Okara and soymilk are separated by a centrifugal separator, and the soymilk is adjusted to pH with sulfuric acid. After adjusting to 4.5, centrifuged and separated into isoelectric protein and whey protein.After adding 4 times water to isoelectric protein and adjusting to pH 6.0 with NaOH. Then, 50 parts by weight of an 8% concentration soybean protein solution was prepared.
• This soybean protein solution was heated to 50 ° C., and 0.04 parts by weight of phytase-degrading enzyme “Sumiteam PHY” manufactured by Shin Nippon Chemical Co., Ltd. was added and reacted for 60 minutes.
• the reaction solution was sterilized at 150 ° C for 7 seconds, cooled to 50 t, adjusted to pH 7.0 with Na ⁇ H, and proteases ”from Amano Pharmaceutical Co., Ltd. 0.16 Parts by weight were added and reacted for 5 hours.
• the pH was adjusted to 6.5, sterilized at 150 ° C for 7 seconds, and immediately powder-dried with a spray dryer.
• the content of phytic acid (mesoinositoxyhexalic acid) in this powder was not detected when measured by vanadomolybdic acid absorption spectrophotometry (detection limit: 5 mg Z100 g).
• the average molecular weight measured by electrophoresis was about 500.
• a low-phytin soybean protein hydrolyzate produced in the same manner as in Production Example 1 was used as an experimental feed in the aquaculture feed of Table 1 in an amount of 0, 2.5, 5.0, 10.0, and 20.0 parts by weight of casein. And added as shown in Table 1 to obtain a fine particle feed by a conventional method.
• Table 1 shows the composition of the experimental feed. The unit is parts by weight.
• Group 5 is the control.
• the experiment was started at the age of 39 days, and the animals were fed eight times an hour from 9:00 am until 16 o'clock, and at 17 o'clock, fed Artemia as a biological feed at nine times a day.
• the feed amount was 0.31 g-0.50 g / time for Z fish, and 55 to 80 animals / time for Artemia, as the age of day passed.
• Table 2 shows the results of Example 1 and Comparative Example 1.
• Example 1 From the results of Example 1, it was found that the growth of the 14 group to which the low-phytate soybean protein hydrolyzate was added was promoted as compared to the 5 group of the control, and a significant difference was observed particularly in the 13 group. . The survival rate was better in the 14 group than in the 5 group. The pigment abnormality rate did not differ between the groups, and no abnormality was observed in the group to which the soybean protein hydrolyzate was given.
• An acrylic weakly basic anion exchange resin (“KA890” manufactured by Sumitomo Chemical Co., Ltd.) is packed into a column with a diameter of 1.4 cm to a height of 15 cm (resin volume 23 cm2), and 50 ml of a 5% sodium hydroxide solution and ion exchange. Washing was carried out by passing 500 ml of water.
• the above-mentioned enzyme hydrolyzate (SH) was dissolved in ion-exchanged water, the pH was adjusted to 4.5 with 10% hydrochloric acid, and adjusted with ion-exchanged water so that the final protein concentration was 10%. .
• the above-prepared solution was passed at 51 ml / hr from the top of the column which had been filled with KA890 and washed, and the treatment solution eluted from the bottom of the column was collected. Pass the enzymatic digestion solution through until the volume reaches 1219 ml (121.9 g protein, 5.3 g per ml resin), that is, phytic acid is completely converted to resin.
• the eluate that had been adsorbed and removed was collected and freeze-dried to obtain a low phosphorus content enzyme digest (SHR).
• Phytic acid content was measured by the method of Mohammed et al. (Cereal Chem. 63. 475.1986). As a result, phytic acid was not detected (detection limit: 0.005% by weight).
• Feeding rate (mg feed / Larva / day)
• nl, n2, n3, n4, n5, n6 and n7 are Zoea 1, Zoea2,
• the experimental feed was prepared based on a commercially available flounder feed (EP feed manufactured by Higashimaru Co., Ltd., S-6 for seeds and seedlings). That is, a commercially available feed is heated, and the low-phytin soybean protein hydrolyzate produced in Production Example 1 and the unfiltered soybean protein hydrolyzate produced in Production Example 2 are attached thereto. After coating, it was dried and used as experimental feed. Table 6 shows the composition of commercial feed and the composition of experimental feed supplemented with soybean protein hydrolyzate.
• the water temperature, feeding amount, and feeding status during the rearing period are described below.
• Water is added seven times to 10 kg of defatted soybeans, extracted after stirring at 50 ° C and pH 7 for 30 minutes, and then separated from okara and soymilk by a centrifugal separator. After adjusting to 4.5, centrifuged and separated into isoelectric precipitate protein and whey protein.After adding 4 times water to the isoelectric precipitate protein, adjusting the pH to 7.0 with NaOH, 50 liters of an 8% protein solution was prepared.
• proteolytic enzymes “proteases” and “protease MJ (manufactured by Amano Pharmaceutical Co., Ltd.) were added, reacted at 50 ° C. for 5 hours, and hydrolyzed (1). Then, the pH was adjusted to 6.0, and 40 g of phytate-degrading enzyme “Sumitim P HY” (manufactured by Shin Nippon Chemical Co., Ltd.) was added. After the addition, the mixture was reacted at 50 ° C for 60 minutes, adjusted to pH 6.5 with NaOH, sterilized at 150 ° C for 7 seconds, and immediately dried with a spray dryer.
• Water is added 7-fold to 10 kg of defatted soybeans, extracted with stirring at 50 and pH 7 for 30 minutes, and then separated into okara and soymilk by a centrifugal separator. After adjusting to 5 and centrifuging to separate the isoelectric precipitate protein and whey protein, add 4 fold water to the isoelectric precipitate protein, adjust to pH 6.0 with Na ⁇ H, and adjust to 8% A concentration of 50 liters of protein solution was adjusted.
• This protein solution was heated to 50 ° C., and lipase-degrading enzyme “Sumitim PHY” was added with 40 g from Shin Nippon Chemical Co., Ltd., and allowed to react for 60 minutes.
• the pH was adjusted to 7.0 with H, and 160 g of “Proteases” (Amano Pharmaceutical Co., Ltd.) was added and reacted for 5 hours.
• the pH was adjusted to 6.5, sterilized at 150 ° C for 7 seconds, and immediately dried with a spray drier.
• the content of phytic acid (mesoinosittohexalic acid) in this powder was not detected by vanadomolybdic acid absorption spectrophotometry (detection limit: 5 mg / 100 g), reducing phytic acid extremely well did it.
• Water is added 7-fold to 10 kg of defatted soybeans, extracted at 50 :, pH 7 with stirring for 30 minutes, separated into okara and soymilk by a centrifuge, and the soymilk is PH4 After adjusting the pH to 5, centrifuged and separated into isoelectric precipitate protein and whey protein.After adding 4 fold water to the isoelectric precipitate protein, adjusting the pH to 6.0 with NaOH, 8 50 liters of a 50% protein solution was adjusted and immediately powder-dried with a spray dryer.
• This protein solution was adjusted to an 8% solution, heated to 50 ° C, added with 40 g of Sumitomo P HY, a protease degrading enzyme, manufactured by Shin Nippon Chemical Co., Ltd., and reacted for 60 minutes. After the reaction, the pH was adjusted to 7.0 with NaOH, and 160 g of "Proteases" (manufactured by Amano Pharmaceutical Co., Ltd.) was added, followed by reaction for 5 hours. The pH was adjusted to 6.5, sterilized at 150 ° C for 7 seconds, and immediately dried with a spray drier.
• This protein solution was heated to 50 ° C, and the phytase-degrading enzyme “Sumitim PHY”, 40 g, manufactured by Shin Nippon Chemical Industry Co., Ltd. was added and reacted for 60 minutes. Was dried with a spray dryer.
• the solution was adjusted to 8%, adjusted to pH 7.0 with NaOH, and added with 160 g of Proteaze M "Amano Pharmaceutical Co., Ltd., and allowed to react for 5 hours. After sterilization for 7 seconds, the powder was immediately dried with a spray dryer.
• Water is added seven times to 10 kg of defatted soybeans, extracted after stirring at 50 ° C and pH 7 for 30 minutes, and then separated from okara and soymilk by a centrifugal separator. After adjusting to 4.5, centrifuged and separated into isoelectric precipitate protein and whey protein.After adding 4 times water to the isoelectric precipitate protein, adjusting the pH to 6.0 with NaOH, 50 liters of an 8% protein solution was prepared.
• the content of phytic acid (mesoinosittohexalic acid) in this powder was measured by vanadomolybdic acid spectrophotometry, and it was found that phytic acid was reduced to 0.2% (detection limit: 5 mg / 1 0 0 g).
• the decomposition solution was adjusted to a high-speed centrifugal separator capable of continuous processing at a feed speed of 100 liters, and the sedimentation component generated was separated and removed.
• the obtained centrifugal supernatant (solid content: 70%) was adjusted to pH 6.5, sterilized at 150 ° C for 7 seconds, and immediately dried by a spray dryer.
• the content of phytic acid (mesoinosittohexalic acid) in this powder was not detected by vanadomolybdic acid absorption spectrophotometry (detection limit: 5 mg / 100 g). Can be reduced.
• the growth of larvae and larvae By feeding the feed of the present invention, the growth of larvae and larvae can be promoted, and the survival rate can be greatly increased.
• the low-phytin vegetable protein hydrolyzate having an extremely low phytic acid content and being below the detection limit by vanadomolybdic acid absorption spectrophotometry has become possible.
• the low-phytin vegetable protein hydrolyzate according to the method of the present invention has an excellent flavor (it is presumed to be due to the decomposition of phytic acid) as compared with the low-phytin soybean protein hydrolyzate obtained by the resin adsorption method.
• the low-phytin plant protein hydrolyzate of the present invention has a small molecular weight and extremely low phytic acid, so that it can be digested and absorbed when used in fry with insufficient development of digestion and absorption functions or animal feeds immediately after birth. It is highly effective and promotes the absorption of trace metals such as calcium.

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• 2003
  • 2003-07-23 JP JP2005501227A patent/JP4556868B2/ja not_active Expired - Fee Related
  • 2003-07-23 CN CNB038177072A patent/CN100379354C/zh not_active Expired - Fee Related
  • 2003-07-23 KR KR1020047010738A patent/KR101042124B1/ko active IP Right Grant
  • 2003-07-23 AU AU2003248093A patent/AU2003248093A1/en not_active Abandoned
  • 2003-07-23 CN CN2007101081985A patent/CN101066097B/zh not_active Expired - Fee Related
  • 2003-07-23 KR KR1020117006214A patent/KR20110033314A/ko not_active Application Discontinuation
  • 2003-07-23 WO PCT/JP2003/009355 patent/WO2004017751A1/ja active Application Filing

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