WO2001049308A1 - Nouvelles compositions de renforcement de l'immunité - Google Patents
Nouvelles compositions de renforcement de l'immunité Download PDFInfo
- Publication number
- WO2001049308A1 WO2001049308A1 PCT/JP2000/009383 JP0009383W WO0149308A1 WO 2001049308 A1 WO2001049308 A1 WO 2001049308A1 JP 0009383 W JP0009383 W JP 0009383W WO 0149308 A1 WO0149308 A1 WO 0149308A1
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- WIPO (PCT)
- Prior art keywords
- matsutake
- extract
- hot water
- alkali solution
- water extract
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Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/06—Fungi, e.g. yeasts
- A61K36/07—Basidiomycota, e.g. Cryptococcus
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L31/00—Edible extracts or preparations of fungi; Preparation or treatment thereof
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/105—Plant extracts, their artificial duplicates or their derivatives
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L5/00—Preparation or treatment of foods or foodstuffs, in general; Food or foodstuffs obtained thereby; Materials therefor
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P35/00—Antineoplastic agents
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P37/00—Drugs for immunological or allergic disorders
- A61P37/02—Immunomodulators
- A61P37/04—Immunostimulants
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P39/00—General protective or antinoxious agents
- A61P39/06—Free radical scavengers or antioxidants
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2200/00—Function of food ingredients
- A23V2200/30—Foods, ingredients or supplements having a functional effect on health
- A23V2200/324—Foods, ingredients or supplements having a functional effect on health having an effect on the immune system
Definitions
- the present invention relates to novel immunopotentiating compositions.
- the immunopotentiating composition of the present invention can be administered not only as a pharmaceutical product but also in various forms, for example, as a functional food or a health food (including beverages), or as a feed in the form of a food or drink.
- it may be given in the mouth temporarily, but most of it is exhaled from the mouth, for example, in the form of a dentifrice, mouthwash, chewing gum, or mouthwash, or it may be sucked through the nose. It can also be given in the form of an inhalant.
- Japanese Patent Publication No. 57-12330 and Japanese Patent No. Discloses various anti-tumor substances contained therein.
- the Japanese Patent Publication No. 57-12330 discloses that emitanine mono-5-A, which is separated and purified from an extract obtained by extracting a liquid culture of a mattella mycelium with hot water or a dilute solution of Arikari, It is disclosed that Emitanin- 15-B, Emitanine- 15-C, and Emitanine- 15- have an inhibitory effect on the proliferation of sarcoma 180 cells. Also, in the specification of Japanese Patent No.
- the present inventor compared various physiological activities of the hot water extract of various commercially available edible mushrooms, and found that in many edible mushrooms including Matsutake mushroom, the proliferation of sarcoma 180 cells was inhibited. While the activity was observed, it was newly found that only matsutake showed a very high activity with regard to the immunopotentiating activity.
- Matsutake hot water extract but also Matsutake alkali solution extract or matttake hot water extract or mattake alkali solution extract, anion-exchange resin adsorption fraction Have also found that they exhibit immunopotentiating activity.
- the present invention relates to an anion-exchange resin-adsorbed fraction of Matsutake (Tricholomamamassutake) hot water extract or Matsutake alkali solution extract.
- Matsutake Tricholomamamassutake
- the present invention relates to a matsutake hot water extract or a matsutake alkali solution extract, or a matsutake hot water extract or a mattake alkali solution extract, which is adsorbed on an anion exchange resin, and pharmaceutically acceptable.
- An immunopotentiating composition a killer activity-inducing (preferably inducing killer activity of intestinal lymphocytes) composition, a tumor growth-inhibiting composition, an interleukin-12-inducing composition, a TGF- /? Activity And an active oxygen scavenging composition.
- the present invention provides a Matsutake hot water extract or Matsutake alkali solution extract, or a mattake hot water extract or Matsutake alkali solution extract, which is used alone to extract a fraction-exchange resin-adsorbed fraction.
- an immunopotentiating functional food a killer activity-inducing (preferably, killer activity-inducing of intestinal lymphocytes) functional food, a tumor growth-inhibiting functional food, an intact food, optionally containing one or more food components
- the present invention relates to a leukin 12-inducing functional food, a TGF- /? Activity-suppressing functional food, and an active oxygen-scavenging functional food.
- the present invention relates to a matsutake hot water extract or a matsutake alkaline solution extract, or an anion-exchange resin-adsorbed fraction of a mattatake hot water extract or a matsutake alkali solution extract, which is a target requiring immunity enhancement.
- a method of enhancing immunity comprising administering an effective amount.
- the present invention relates to a method for inducing killer activity (preferably intestinal tract) of a matsutake hot water extract or a matsutake alkali solution extract, or an anion-exchange resin-adsorbed fraction of a mattake hot water extract or a matsutake alkali solution extract.
- killer activity preferably intestinal tract
- the present invention relates to a method for inducing killer activity (preferably killer activity of intestinal lymphocytes), which comprises administering an effective amount to a subject of interest.
- the present invention relates to a method for inhibiting tumor growth of a Matsutake hot water extract or Matsutake alkali solution extract, or an anion-exchange resin-adsorbed fraction of Mattake hot water extract or Matsutake alkali solution extract. And a method for inhibiting tumor growth, which comprises administering an effective amount.
- the present invention provides a Matsutake hot water extract or Matsutake alkali solution extract, or a mattake hot water extract or Matsutake alkali solution extract, which is obtained by adsorbing a fraction adsorbed to a Yin exchange resin on Interleukin 1 (2)
- a method for inducing interleukin-12 which comprises administering an effective amount to a subject in need of induction.
- the present invention relates to a method for preparing TGF- / 5 activity, which comprises extracting a matsutake hot water extract or a matsutake alkali solution extract, or a mattake hot water extract or a matsutake alkali solution extract with a Yin exchange resin-adsorbed fraction.
- the present invention relates to a method for suppressing TGF-5 activity, which comprises administering an effective amount to a subject requiring suppression.
- the present invention relates to a method for capturing active oxygen in a matsutake hot water extract or a matsutake alkali solution extract, or an anion-exchange resin-adsorbed fraction of a matsutake hot water extract or a mattake alkali solution extract. And a method for scavenging active oxygen, which comprises administering an effective amount.
- the present invention provides a matsutake hot water extract or a matsutake alkali solution extract, or a mattake hot water extract or a matsutake alkali solution extract, which is an immuno-enhancing composition
- the present invention relates to a use for producing an immunopotentiating functional food.
- the present invention provides a method for inducing killer activity (preferably, a matsutake hot water extract or a matsutake alkali solution extract, or a fraction of a mattake hot water extract or a matsutake alkali solution extract, which is adsorbed on a yin-exchange resin.
- killer activity preferably, a matsutake hot water extract or a matsutake alkali solution extract, or a fraction of a mattake hot water extract or a matsutake alkali solution extract, which is adsorbed on a yin-exchange resin.
- the present invention relates to the use of a composition or a killer activity inducer (preferably an intestinal lymphocyte killer activity inducer) for producing a functional food.
- the present invention provides a matsutake hot water extract or a matsutake alkali solution extract, or a mattake hot water extract or a matsutake alkali solution extract, which is an exchange resin.
- the present invention relates to the use of an adsorbed fraction for producing a tumor growth-inhibiting composition or a tumor growth-inhibiting functional food.
- the present invention also relates to an interleukin-12-derived composition
- an interleukin-12-derived composition comprising a Matsutake hot water extract or Matsutake alkali solution extract, or an anion-exchange resin-adsorbed fraction of Matsutake hot water extract or Mattake alkali solution extract. Or an interleukin 12-induced functional food.
- the present invention also relates to a method for suppressing TGF-II activity of a matsutake hot water extract or a matsutake alkali solution extract, or a fraction of a mattake hot water extract or a matsutake alkali solution extract, which is adsorbed on a Yin exchange resin.
- the present invention relates to a composition or a use for producing a TGF- / 5 activity-suppressing functional food.
- the present invention relates to a matsutake hot water extract or a matsutake alkali solution extract, or an active oxygen scavenging composition or an activity of an anion-exchange resin-adsorbed fraction of a mattake hot water extract or a matsutake alkali solution extract. It relates to the use for producing oxygen-scavenging functional foods.
- FIG. 1 shows a spectrum obtained by 1 H-dimensional NMR measurement of the adsorption fraction D 2.
- FIG. 2 is a spectrum obtained by 13 C-dimensional NMR measurement of the adsorption fraction D 2.
- FIG. 3 is a CD spectrum obtained by circular dichroism analysis of the adsorption fraction D2. BEST MODE FOR CARRYING OUT THE INVENTION
- the novel fraction of the present invention which can be obtained by hot water extraction from matsutake (Tricholomamatsutake) and adsorbing an anion exchange resin, has excellent immunopotentiating activity despite the hot water extraction step. Show. Also, Matsutake hot water extraction Exudation also shows excellent immunopotentiating activity. Furthermore, the extract of Matsutake alkali solution also exhibits excellent immunopotentiating activity, and the novel fraction of the present invention, which can be obtained by adsorbing the alkaline solution extract with an anion exchange resin, also exhibits excellent immunopotentiating activity.
- the immune enhancing composition according to the present invention comprises: (1) Matsutake hot water extract, (2) Matsutake alkali solution extract, (3) Matsutake hot water extract anion exchange resin-adsorbed fraction, or ( 4) Anion-exchange resin-adsorbed fraction of matsutake alkali solution extract contains as an active ingredient, and also contains a normal carrier that is pharmaceutically or veterinarily acceptable.
- the anion-exchange resin-adsorbed fraction of the Mattakeh hot water extract which is the active ingredient in the immunopotentiating composition of the present invention, is not limited to this.
- Matsutake is extracted with hot water (hereinafter referred to as “Matsutake”).
- an anion exchange resin adsorption step After the resulting extract is adsorbed on an anion exchange resin (hereinafter referred to as an anion exchange resin adsorption step), the adsorbed fraction is eluted with a suitable eluent. (Hereinafter, referred to as an elution step).
- the matsutake used in the hot water extraction step includes, for example, a natural fruit body or mycelium of matsutake, or a mycelium or culture of matsutake obtained by culturing.
- Matsutake mushroom used for culture examples include, for example, Matsutake CM627-1 strain established and maintained by Kureha Chemical Industry Co., Ltd. Biomedical Research Laboratory.
- the temperature of the hot water used in the hot water extraction step is preferably 60 to 100 ° C, more preferably 80 to 98 ° C.
- the extraction time depends on, for example, the state of matsutake (ie, whether it is in a fruiting body, a mycelium, or a culture, or in the case of processing into a crushed or powdered state), It can be appropriately determined according to the temperature of the hot water, the presence or absence of stirring or shaking, or the conditions, but is usually 1 to 6 hours, preferably 2 to 3 hours.
- the extract obtained in the hot water extraction step is mixed with insolubles, It can be used in the anion exchange resin adsorption step, but after removing the insoluble matter, or after removing the insoluble matter, and further removing the low molecular fraction in the extract, It is preferably used in the ion exchange resin adsorption step.
- the insolubles are removed by centrifuging the hot water extract containing the insolubles, and only the resulting supernatant can be used in the next anion exchange resin adsorption step.
- the supernatant obtained by centrifuging the hot water extract containing insolubles is dialyzed to remove a low-molecular fraction (preferably a fraction having a molecular weight of 350 or less), and then It can be used in the anion exchange resin adsorption step.
- anion exchange resin that can be used in the anion exchange resin adsorption step
- a known anion exchange resin can be used. For example, getylaminoethyl
- D EA E Cell mouth or triethylammonium ethyl (T EA E) cell mouth.
- the eluent used in the elution step can be appropriately determined according to the type of anion exchange resin used in the anion exchange resin adsorption step, and examples thereof include an aqueous solution of sodium chloride.
- the fraction eluted in the elution step can be used as it is as an active ingredient of the immunopotentiating composition of the present invention, but usually contains a salt derived from the eluate.
- dialysis is performed.
- the extract of the matsutake hot water extract which is the active ingredient in the immunopotentiating composition of the present invention, is not limited thereto, but has, for example, the following physicochemical properties.
- the numerical values shown below are physicochemical properties of the adsorption fraction D 2 prepared in Example 2 described later, which is an embodiment of the anion exchange resin adsorption fraction of Matsutake hot water extract, and described below. This is a numerical value based on each measurement method described in “Examination of physicochemical properties of adsorption fraction D2” in Examples.
- Carbohydrate content 13% in terms of glucose.
- Molecular weight distribution widely distributed between about 450,000 and $ 1,000. Among them, there are five peak-tops, and their estimated molecular weights are about 450,000, about 120,000, about 160,000, about 380000, and more than 100,000.
- Isoelectric point Three peaks are detected by isoelectric focusing. The main band is around 4.8 (4.5 to 5.2), and the other bands are around 7.6
- Circular dichroism analysis shows the spectrum shown in Fig. 3 [For the measurement conditions, see “Study on physicochemical properties of adsorbed fraction D2" (9) described later).
- the secondary structure was analyzed according to the method of Chen et al. [Biochemistry, 11, 4, 12 0-4 13 1 (1972)], helix 1,%, ⁇ sheet 18 %, And irregular structure 65%.
- the active ingredient in the immunopotentiating composition of the present invention is not limited thereto.
- matsutake is extracted with an alkaline solution (hereinafter referred to as an alkaline solution extraction step), and after the obtained extract is adsorbed on an anion exchange resin (that is, an anion exchange resin adsorption step), an appropriate eluent is obtained.
- the adsorption solution can be obtained by eluting the adsorbed fraction (ie, the elution step).
- the alkali solution used in the extraction step is not limited to this.
- An aqueous solution of a hydroxide for example, sodium or potassium
- an aqueous solution of sodium hydroxide can be used.
- the pH of the alkaline solution is preferably from 8 to 13, more preferably from 9 to 12, and more preferably from 9 to 12.
- the alkaline solution extraction step is preferably performed at 0 to 20 ° C, more preferably at 0 to 15 ° C.
- the extract obtained in the alkali extraction step can be subjected to a neutralization treatment and then used in the next step of adsorbing the exchange resin.
- the immunity enhancing composition of the present invention comprises a matsutake hot water extract or mattake alkali solution extract as an active ingredient, or an anion exchange resin-adsorbed fraction of a matsutake hot water extract or matsutake alkali solution extract, as a drug. It can be administered to animals, preferably mammals (particularly humans), together with conventional carriers that are chemically or veterinarily acceptable.
- the active ingredient of the present invention has an immunopotentiating activity, for example, killer Activity (preferably, killer activity of intestinal lymphocytes), tumor growth inhibitory activity, cytokine (especially interleukin-12) inducing activity, TGF- ;? It has activity suppression activity or active oxygen scavenging activity.
- killer Activity preferably, killer activity of intestinal lymphocytes
- cytokine especially interleukin-12
- the active ingredient of the present invention Matsutake hot water extract or Matsutake alkali solution extract, or Matsutake hot water extract or Matsutake alkali solution extract, the anion-exchange resin-adsorbed fraction alone or A subject in need of immune enhancement, preferably a conventional carrier that is pharmaceutically or veterinarily acceptable, eg a subject in need of induction of killer activity (preferably killer activity of intestinal lymphocytes) , Effective dose for subjects requiring tumor growth suppression, subjects requiring induction of cytodynamics (particularly interleukin 12), subjects requiring suppression of TGF-II activity, or subjects requiring active oxygen capture Can be administered.
- the active ingredient of the present invention is an immunopotentiating composition.
- a functional food for enhancing immune function for example, a composition for inducing killer activity (preferably for inducing killer activity of intestinal lymphocytes) or a functional food for inducing killer activity (preferably for inducing killer activity of intestinal lymphocytes), a functional food, or a composition for suppressing tumor growth Product or tumor growth-inhibiting functional food, interleukin-12-inducing composition or interleukin-12-inducing functional food, TGF- /? Activity-inhibiting composition or TGF- /? Activity-inhibiting functional food, or active oxygen It can be used to produce a scavenging composition or an active oxygen scavenging functional food.
- the dosage form of the immunopotentiating composition according to the present invention is not particularly limited, and examples thereof include powders, fine granules, granules, tablets, capsules, suspensions, emulsions, syrups, extracts, and pills. And parenteral preparations such as injections, topical solutions, ointments, suppositories, creams for topical administration, or eye drops.
- oral preparations include, for example, gelatin, sodium alginate, starch, corn starch, sucrose, lactose, glucose, mannitol, carboxymethylcellulose, dextrin, polyvinylpyrrolidone, crystalline cellulose, soy lecithin, sucrose, fatty acids Excipients, such as esters, talc, magnesium stearate, polyethylene glycol, magnesium silicate, carboxylic anhydride, or synthetic aluminum silicate, binders, disintegrants, surfactants, lubricants, and glidants It can be manufactured according to a conventional method using a diluent, a preservative, a coloring agent, a flavor, a flavor, a stabilizer, a humectant, a preservative, an antioxidant, or the like.
- parenteral administration methods include injection (subcutaneous, intravenous, etc.) and rectal administration. Of these, injections are most preferably used.
- a water-soluble solvent such as physiological saline or Ringer's solution
- a water-insoluble solvent such as vegetable oil or fatty acid ester, glucose or sodium chloride Etc.
- isotonic agents solubilizing agents
- the immunopotentiating composition according to the present invention may be administered using a sustained-release preparation technique using a sustained-release polymer or the like.
- the immunopotentiating composition according to the present invention can be incorporated into a pellet of ethylene vinyl acetate polymer, and the pellet can be surgically implanted into the tissue to be treated or prevented.
- the immune enhancing composition according to the present invention includes, but is not limited to, Matsutake hot water extract or Matsutake alkali solution extract, or Matsutake hot water extract or Matsutake alkali solution extract anion exchange resin.
- the adsorbed fraction may be contained in an amount of 0.01 to 99% by weight, preferably 0.1 to 8% by weight.
- the dose when using the immune-enhancing composition according to the present invention can be appropriately determined according to the type of disease, age, sex, body weight, degree of symptoms, degree of symptoms, administration method and the like of the patient. Alternatively, it can be administered parenterally.
- the form is not limited to pharmaceuticals, and it can be provided in various forms, for example, functional foods and health foods (including beverages), or as feeds and drinks. Furthermore, it is contained in the mouth temporarily, but most of it is exhaled from the mouth, for example, in the form of a dentifrice, mouthwash, chewing gum, or gargle, or in the form of an inhalant to be inhaled through the nose It is also possible to give in.
- a matsutake hot water extract or matsutake alkali solution extract, or an anion exchange resin-adsorbed fraction of a matsutake hot water extract or mattake alkali solution extract is used as an additive (for example, a food additive). It can be added to desired foods (including beverages), feeds, dentifrices, mouthwashes, chewing gums, or gargles.
- Table 1 shows the types and yields of the mushrooms (based on dry cell mass%).
- the edible mushroom extract obtained as a powder in the previous section was orally administered to mice, and the effect on the killer activity induction of intestinal lymphocytes was examined. That is, the mesenteric lymph node cells were removed from the mouse in which the tumor cells were transplanted to the cecal wall, and the biological activity was assayed by measuring the killer activity when restimulating with the tumor cells in a test tube.
- the tumor cells used were murine leukemia cells P815 and B7 / P815, which were obtained from a 10% bovine cell line donated by Dr. Mamoru Harada, Kyushu University Institute for Biomedical Defense. Fetal serum (heat-treated at 56 ° C for 30 minutes) supplemented Maintained in KPMA Chemical Industry Co., Ltd. Biomedical Research Laboratory in RPMI164 medium.
- the animals were female DBAZ2 mice purchased from Japan SLC, which were preliminarily reared and used in experiments at the age of 8 weeks.
- Pentobarbital (Dainippon Pharmaceutical) 50 mgZkg was intraperitoneally administered to fix an anesthetized mouse, the abdomen was opened with scissors and tweezers, and the cecum was removed. using dental needle (banner Motoki needle) 1 m L Yochu morphism tube fitted with a, B 7ZP 8 1 5 cells (1 X 1 0 6 or 5 0 mu L) moves under cecum wall The plants were planted and the abdomen was closed using a dissecting stapler. The mice anesthetized were placed in a rearing cage and reared in a normal rearing environment. From the day after the tumor cell transplantation, 500 mg / kg / kg / day of each sample was orally administered for 10 consecutive days using a sonde for oral administration (5 to 10 animals per group).
- mice On the day after the final administration, the mice were sacrificed, the mesenteric lymph nodes were aseptically removed, and transferred to a sterile dish containing Hanks Balanced Salt Solution. After loosening the lymph nodes with scissors and tweezers, a single cell solution of lymphocytes was prepared through a mesh.
- P 8 1 5 cell or B 7 /? 8 1 5 Cells 1 0% fetal bovine serum (56 ° heat-treated for 30 minutes at C) 5X 1 0 6 or in addition RPMI 1 64 0 medium / m And then add mitomycin C (Sigma) to 50> ugZmL and react in a 5% carbon dioxide incubator for 30 minutes, then add 10% fetal calf serum (Sigma). 5 heat treated 6 ° C for 3 0 minutes) cells were washed 3 times with added R PM I 1 6 4 0 medium, the cell concentration was adjusted to 1 X 1 0 5 or L.
- the mixed lymphocyte-tumor cell reaction (MixedLymphoccyteTumorccellReaction) was examined under the following conditions.
- a 96-well flat bottom microplate for cell culture (FaIcon 3072; Becton Dickinson Labware, USA) is prepared by adding 0.1 ml of suae!
- the cells were cultured for 3 days in a 5% carbon dioxide incubator, and the cells were collected on a filter.
- the ratio of the number of both was set to 12.5 (the number of effecta cells / stimulator cells).
- the effector cells function as “lymphocytes” in a mixed lymphocyte-tumor cell reaction
- the stimulator cells function as “tumor cells”.
- 3 H-thymidine (Amersham Japan) 37 kBq was added to each well of the plate.
- the collected cells were thoroughly washed with 5% trichloroacetic acid, dried, placed in a liquid vial, a liquid scintillator was added, and the radioactivity was measured with a liquid scintillation counter.
- Stimulation 1 tex (StimuIationIndex; S.I.) is expressed by the formula:
- lymphocyte mixed cell-induced cytotoxicity was examined under the following conditions.
- a 24-well cell culture microplate (CuIture CI astar) (Costar 3 524; Corning Inc., USA).
- Bmax the maximum free Group radioactivity
- the spontaneous free group refers to a group in which only radioactive chromium-labeled tumor cells are cultured, and the largest free group refers to Triton-treated radiochromium-labeled tumor cells. It means a group of tumor cells.
- each of the obtained non-adsorbed fraction and adsorbed fraction solutions was put into a dialysis membrane (Spectra / Po 3 Membrane, molecular weight: 3500 fraction), and dialyzed in pure water for 3 days.
- the dialysis membrane inner solution was concentrated by a single evaporator and freeze-dried to obtain 1.9 g of a non-adsorbed fraction D1 powder and 1.5 g of an adsorbed fraction D2 powder.
- Lymphocytes ⁇ Tumor cells mixed lymphocytes • Tumors Cytotoxicity by mixed culture Cell response Activity induction reaction
- the sugar content of the D2 fraction was 13% in terms of glucose.
- the protein content of the D2 fraction was 86% in terms of albumin.
- the sugar composition is, in order of increasing content, mannose 47.7 yu gZmg, galactose 32.3 g, mg glucose 25.09 gZmg, arabinose 12.09 gZmg, ribose 8.3 0 g / mg, xylose 3.75 g / mg, and rhamnose 0.449.
- Amino acid composition is aspartic acid and asparagine 14.12 mo 1%, torenin 6.39 mol%, serine 7.64 mol%, glutamic acid and glutamin 13Z8 3 mol%, glycine 14 0 3 mol%, alanine 7.7 7 mo
- the molecular weight distribution was widely distributed between about 450,000 and 1,000,000. Peak in it Five tops were found, each with an estimated molecular weight of about 450,000, about 120,000, about 160,000, about 380000, and more than 100,000.
- Electrophoresis buffer (cathode) 0.04 mo I ZL sodium hydroxide solution, (anode) 0.
- Electrophoresis conditions electrophoresis was performed at 100 V for 30 minutes, electrophoresis was then performed at 300 V for 20 minutes, and electrophoresis was performed at 500 V for 40 minutes.
- the main band is around 4.8 (4.5 to 5.2), and around 7.6 (7.35 to 8.0) and around 9.2 (8.65 to 9.3). There was a band.
- the measurement conditions are as follows.
- UNITY I NOVA-500 type (Varian) was used. Using D of hydrochloric Guanijiumu D 2 0 solution as a solvent. The concentration was 4 m 0 I ZL, and the measurement was carried out at 23 ° C using DSS as an internal standard. Fig. 1 shows the obtained spectrum. A peak, which is considered to be the o-position of galactose or glucose, was observed at around 4.5 ppm to 5. Op pm, which is characteristic of the 1st-proton of the sugar. In addition, a peak which is considered to be the ⁇ 1st place of galactose or glucose was observed at around 5.0 to 5.2 ppm.
- the result is shown in figure 2.
- the broad peak around 103-104 ppm is considered to be a glucosidic bond in galactose or glucose at the 1-position carbon. It is considered that the peaks at 10.2.96 ppm and 102.51 ppm are linked by a curcoside bond at the 1st and / or 1st positions of mannose, respectively. It is considered that the peak at 99.17 ppm is at the first position of galactose or glucose and is also a glucoside bond.
- the measurement conditions are as follows.
- JASCOJ-500OA was used as a measuring device, and water was used as a solvent.
- the protein concentration is 0.125 mg gZmL
- the wavelength range is 200-250 nm
- the cell length is 1 mm
- the temperature is room temperature (at 24)
- the number of integrations is The measurement was performed under the condition of eight times.
- Figure 3 shows the obtained CD spectrum.
- the CD value (vertical axis) is represented by the average residue ellipticity [].
- the unit of [] is deg ⁇ cm 2 ⁇ decimo I- 1 .
- the average molecular weight of the residue used for conversion to ⁇ was 103.45. Determined).
- the secondary structure was analyzed according to the method of Chen et al., And it was found that a-helix was 17%,? -Sheet was 18%, and irregular structure was 65%.
- Table 4 shows the results. * Indicates that there is a significant difference at p ⁇ 0.05 against the control group. As is clear from Table 4, the administration of the D2 fraction significantly inhibited the growth.
- DBAZ2 mice Eight-week-old female DBAZ2 mice were sacrificed after sacrifice, mesenteric lymph nodes were aseptically removed, and transferred to a sterile Petri dish containing Hanks balanced salt solution. Scissors and pincer The lymph nodes were loosened with a sample, and a single cell solution of lymphocytes was prepared through a mesh. After washing the cells 3 times with 1 0% fetal calf serum (5 6 heat-treated for 30 minutes at C) added R PM I 1 6 4 0 medium was adjusted cell numbers 2 X 1 0 6 or m L . The adsorptive fraction D2 obtained in Example 2 was dissolved in a medium and sterilized with a filter to prepare 250 g of a sample solution.
- TGF- ⁇ content in the reaction solution is measured using a commercially available measurement kit (Quantikine Human TGF). ⁇ , ELISAK it; Funakoshi).
- Table 6 shows the results. In the D2 fraction-added group, binding between the D2 fraction and TGF?, was observed.
- the Swiss Albino 3T3 strain maintained in a 50 mL cell culture flask (31014; Becton—Dickinson) at the Kurewa Chemical Industry Co., Ltd. SV40 transformed 3T3 cell line [obtained from Dainippon Pharmaceutical Co., Ltd. Laboratory Products Department (Osaka)] was treated with 0.125% tribcine solution (Sigma) for several minutes to form a flask. Released from the wall.
- DMEM DuI beccos Modification of Eagles Medium
- fetal serum treated at 56 ° C for 30 minutes
- 2 ⁇ 10 3 Zm After adjusting the volume to 0.1 L, 0.1 mL was dispensed to each well of a 96-well flat bottom microtest plate for cell culture (3072; Becton-Dickinson).
- a predetermined amount of the adsorption fraction D2 obtained in Example 2 or 0.1 mL of the medium was added, and further 24 hours. Cultured. Incidentally, the end 6 hours preculture, 3 H- thymidine (Am ersham I nternationalpic)] was added M C ⁇ wells. After completion of the culture, the cells were collected on filter paper, washed thoroughly with 5% acetic acid trichloride, dried, and the radioactivity incorporated into the cells was measured with a liquid scintillation counter.
- Matsutake CM 627-1 strain mycelium culture prepared in the same manner as in Example 2 was subjected to a homogenizer, and a sodium hydroxide solution was added until the total concentration became 0.1 mo. Extraction was performed at 22 ° C. for 1 hour under stirring with a stirrer. After the extraction, the supernatant was obtained by centrifugation (12000 rpm, 20 minutes, 4). 0.3 moI 1_ sodium hydroxide solution was added to the precipitate, and the same treatment as above was performed to obtain a supernatant. Further, 0.5 mo I 1_ sodium hydroxide solution was added to the precipitate, and the same treatment as above was performed to obtain a supernatant.
- fraction A a powder (hereinafter, referred to as fraction A).
- 20 OmL of a solution obtained by adding 1 mo 11_ sodium chloride to the buffer solution was prepared, applied to the column, and the adsorbate was eluted.
- the adsorbed fraction A 2 (hereinafter simply referred to as “A 2 fraction”) ).
- each of the obtained non-adsorbed fraction and adsorbed fraction solutions was put into a dialysis membrane (SpectraZPOrS Membrane, molecular weight: 3500 fraction), and dialyzed in pure water for 3 days. After the inner solution of the dialysis membrane was concentrated by a single evaporator and freeze-dried, a non-adsorbed fraction A1 powder and an adsorbed fraction A2 powder were obtained.
- SpectraZPOrS Membrane SpectraZPOrS Membrane, molecular weight: 3500 fraction
- the T cell enrichment activity was evaluated by the same method as in the above “Biological activity test of mushroom hot water extract” (2).
- the site force-in inducing activity was evaluated by the same method as in the above “Example of biological activity evaluation of adsorbed fraction D2” (2). While the 12 content was below the detection limit, the total interleukin 12 content in the fraction A-added group was 69 pg Zml.
- TGF-binding activity was evaluated by the same method as in the above “Example of evaluation of biological activity of adsorbed fraction D2” (3).
- the binding activity of fraction A was 26%.
- immunity can be enhanced.
- the present invention has been described in accordance with the specific embodiments. However, modifications and improvements obvious to those skilled in the art are included in the scope of the present invention.
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EP00987787A EP1256351A4 (en) | 2000-01-05 | 2000-12-28 | NEW IMMUNTIMULATING COMPOSITIONS |
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WO2003070264A1 (fr) * | 2002-02-22 | 2003-08-28 | Kureha Chemical Industry Co., Ltd. | Fraction adsorbee sur une resine echangeuse d'anions, immunopotentiateur et promoteur pour sa recuperation a partir du stress applique provenant du champignon matsutake |
JP2004210695A (ja) * | 2002-12-27 | 2004-07-29 | Kureha Chem Ind Co Ltd | 癌予防剤および食品 |
WO2005035571A1 (ja) * | 2003-10-14 | 2005-04-21 | Kureha Corporation | 新規の糖タンパク質及びそれを含有する医薬組成物 |
JP2005162668A (ja) * | 2003-12-02 | 2005-06-23 | Kureha Chem Ind Co Ltd | 新規のTGF−β結合剤 |
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EP1331009A4 (en) * | 2000-10-11 | 2005-04-27 | Kureha Chemical Ind Co Ltd | PHARAMCEUTICAL COMPOSITION FOR RECOVERING OVERLOADING AND NEW MATSUTAKE FUNGUS STRAIN |
JP4618770B2 (ja) * | 2004-02-12 | 2011-01-26 | 株式会社クレハ | 新規な血圧降下剤および食品 |
JP4587441B2 (ja) * | 2004-02-12 | 2010-11-24 | 株式会社クレハ | 新規な糖尿病治療剤および食品 |
JP4587442B2 (ja) * | 2004-02-12 | 2010-11-24 | 株式会社クレハ | 新規な高脂血症治療剤および食品 |
KR100924382B1 (ko) * | 2007-12-07 | 2009-10-30 | (주)머쉬토피아 | 새송이버섯을 이용한 기능성 음료 및 그 제조방법 |
KR101101180B1 (ko) * | 2009-06-12 | 2012-01-05 | 이명헌 | 송이버섯 음료 및 그 제조방법 |
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CN102018731B (zh) * | 2010-11-17 | 2013-04-03 | 河北师范大学 | 一种从色钉菇中提取抗氧化活性物质的方法及其应用 |
CN111411082B (zh) * | 2020-03-26 | 2022-04-01 | 中山大学孙逸仙纪念医院 | 一种培养CD90posi细胞的培养基及其培养方法 |
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- 2000-12-28 WO PCT/JP2000/009383 patent/WO2001049308A1/ja not_active Application Discontinuation
- 2000-12-28 AU AU24046/01A patent/AU2404601A/en not_active Abandoned
- 2000-12-28 KR KR1020027008682A patent/KR20020070377A/ko not_active Application Discontinuation
- 2000-12-28 EP EP00987787A patent/EP1256351A4/en not_active Withdrawn
- 2000-12-28 CA CA002396239A patent/CA2396239A1/en not_active Abandoned
- 2000-12-28 US US10/169,779 patent/US20030044424A1/en not_active Abandoned
- 2000-12-28 CN CNB008182302A patent/CN1198639C/zh not_active Expired - Fee Related
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Cited By (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2003070264A1 (fr) * | 2002-02-22 | 2003-08-28 | Kureha Chemical Industry Co., Ltd. | Fraction adsorbee sur une resine echangeuse d'anions, immunopotentiateur et promoteur pour sa recuperation a partir du stress applique provenant du champignon matsutake |
JP2004210695A (ja) * | 2002-12-27 | 2004-07-29 | Kureha Chem Ind Co Ltd | 癌予防剤および食品 |
WO2005035571A1 (ja) * | 2003-10-14 | 2005-04-21 | Kureha Corporation | 新規の糖タンパク質及びそれを含有する医薬組成物 |
JPWO2005035571A1 (ja) * | 2003-10-14 | 2007-11-22 | 株式会社クレハ | 新規の糖タンパク質及びそれを含有する医薬組成物 |
JP2005162668A (ja) * | 2003-12-02 | 2005-06-23 | Kureha Chem Ind Co Ltd | 新規のTGF−β結合剤 |
Also Published As
Publication number | Publication date |
---|---|
EP1256351A4 (en) | 2003-07-09 |
US20030044424A1 (en) | 2003-03-06 |
CA2396239A1 (en) | 2001-07-12 |
CN1420782A (zh) | 2003-05-28 |
AU2404601A (en) | 2001-07-16 |
KR20020070377A (ko) | 2002-09-06 |
CN1198639C (zh) | 2005-04-27 |
EP1256351A1 (en) | 2002-11-13 |
JP2009209151A (ja) | 2009-09-17 |
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