WO2001028509A1 - Épilatoires et agents pour usage externe - Google Patents
Épilatoires et agents pour usage externe Download PDFInfo
- Publication number
- WO2001028509A1 WO2001028509A1 PCT/JP2000/007218 JP0007218W WO0128509A1 WO 2001028509 A1 WO2001028509 A1 WO 2001028509A1 JP 0007218 W JP0007218 W JP 0007218W WO 0128509 A1 WO0128509 A1 WO 0128509A1
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- hair
- endothelin
- administration
- agents
- depilatories
- Prior art date
Links
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q9/00—Preparations for removing hair or for aiding hair removal
- A61Q9/04—Depilatories
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
- A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- A61K38/17—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- A61K38/1703—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
- A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- A61K38/17—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- A61K38/22—Hormones
- A61K38/2285—Endothelin, vasoactive intestinal contractor [VIC]
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/02—Cosmetics or similar toiletry preparations characterised by special physical form
- A61K8/04—Dispersions; Emulsions
- A61K8/046—Aerosols; Foams
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/64—Proteins; Peptides; Derivatives or degradation products thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P17/00—Drugs for dermatological disorders
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P17/00—Drugs for dermatological disorders
- A61P17/14—Drugs for dermatological disorders for baldness or alopecia
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q7/00—Preparations for affecting hair growth
- A61Q7/02—Preparations for inhibiting or slowing hair growth
Definitions
- the present invention relates to a depilatory and an external preparation useful as medicines, cosmetics and veterinary drugs.
- Mammal head and body hairs biologically protect important organs such as the head, chest, and limbs, but recently, in humans, body hair in limbs and the like has a cosmetic and aesthetic appearance. Absence is preferred and there is a tendency to remove body hair.
- a hair removal method of chemically decomposing hair fibers, or preventing, suppressing or delaying hair growth in addition to a mechanical removal method using a shaver, a hair remover, or the like, is known.
- a hair removal method is liable to cause physical or chemical irritation to the skin, and it cannot be said that it is still sufficient in terms of suppressing hair growth, and the hair removal treatment is performed again after a certain period of time. It is necessary to reduce the hair removal treatment.
- EGF epidermal growth factor
- An object of the present invention is to provide a hair removal agent and an external preparation which are useful as medicines, cosmetics and veterinary medicines by suppressing the growth of body hair on mammalian skin. Disclosure of the invention The inventors of the present invention have studied the substance for hair loss and found that endothelin and its agonist have an excellent hair growth inhibitory action.
- the present invention provides a hair removal agent and an external preparation containing endoselin or an agonist thereof.
- the present invention also provides a hair removal method, which comprises administering endoselin or an agonist thereof.
- FIG. 1 is a graph showing the effect of endoselins on the number of hair follicles during the anagen phase.
- FIG. 2 is a view showing a morphological change of hair follicle tissue by administration of endoselin.
- FIG. 3 is a diagram showing the growth inhibitory effect of endothelin on cultured hair follicles.
- FIG. 4 is a view showing a morphological change of a cultured hair follicle by addition of endothelin.
- Endothelin is a bioactive substance that directly and indirectly continuously contracts vascular and non-vascular smooth muscle.
- the role of endothelin in the formation of individuals has been elucidated, and the role of endothelin in various cells such as glial cells, renal mesangial cells, and melanocytes has been elucidated.
- no effect of endothelin on hair growth has been reported, nor is it known to be used as an external preparation.
- Endothelin is a peptide consisting of 21 amino acid residues, and it is known that three different isoforms of endothelin_1, endothelin-12, and endothelin-3 have been known.
- any isoform can be used, and preferably endothelin-1.
- Each of the isoforms of endothelin described above is activated by the action of an in vivo enzyme (endothelin converting enzyme) from the precursor protein, that is, each big endothelin.
- an in vivo enzyme endothelin converting enzyme
- those precursor proteins can also be used.
- endothelin agonists agonist
- their peptide fragment sequences having agonist action are also useful as the present depilatory agent.
- endothelin agonists Sarafotoxin- 6 C, [Ala 1 '3' 11 '15] - Endothelin - 1 (.... Biochem Biophys Res Co ⁇ un, 182, 144 (1992) ), the Agonisuto action
- fragment sequences of these peptides that have
- I RL 1 620 N- Succinyl- [Glu 9, Ala 1 '15] ET- 1 (8-21)
- BQ3020 N- acetyl- [Ala 11 '15] ET one 1 (6-21)
- Such endothelin can be produced by separation and purification from culture supernatants of endothelin-producing cells or their disrupted solutions, chemical peptide synthesis, or genetic engineering techniques using DNA having a nucleotide sequence encoding endothelin. .
- the hair removal effect of endoselin in the present invention is based on the action of suppressing hair growth by shifting the hair follicles in the anagen phase to the catagen phase as shown in Example 3 below. Therefore, the depilatory agent and the external preparation of the present invention can be used for humans or any livestock animal species, and the type and site of the target hair are not limited.
- the method of administration of the depilatory agent of the present invention can be selected from transdermal administration, local intradermal administration, systemic administration, and the like, depending on the purpose, application target, or site.
- the content of endothelin is usually preferably 0.01 to 10% by weight from the viewpoints of the depilatory effect, economy and the like. Particularly preferred is 1 to 1% by weight.
- the content of endothelin is usually preferably from 0.1 to 100 gZ, particularly preferably from 1 to 10 / g / time.
- 0.01 to: L 0 O mg is preferable, and 0.1 to 10 mg / kg of body weight is particularly preferable.
- subcutaneous or intraperitoneal administration is effective systemically and intradermal administration is effective locally.
- continuous transdermal administration is also effective, but in this case, the one in which transdermal absorption is enhanced by encapsulation in ribosomes is more effective (RM.Hoffman, J Drug Target, 5, 67 ( 1998)).
- a component having a hair control and depilatory action such as a keratolytic agent or thioglycolic acid or a salt thereof can be appropriately added.
- the keratolytic agent include lactic acid, bioprase, salicylic acid, glycolic acid, citric acid, malic acid, and the like.
- thioglycolic acid salts include, for example, sodium salt, potassium salt, ammonium salt, and the like.
- Alnolic amine salts such as monoethanolamine, genoaluminamine, and triethanolamine are exemplified.
- the content of these keratolytic agents, thioglycolic acid or salts thereof is preferably from 0.01 to 10% by weight, particularly preferably from 0.05 to 5%.
- the depilatory agent and the external preparation of the present invention are used as medicines, cosmetics or veterinary medicines, and are preferably cosmetics or medicines related to hair removal, hair removal or shaving.
- Such cosmetics or medicines include hair removers such as pastes, creams, aerosols, etc., hair removers such as box, jewels, sheets, etc., lotions used for hair removal or post-treatment of hair removal.
- Post treatment for creams, etc. deodorant lotion, deodorant powder, deodorant spray, deodorant sticks, etc.
- the depilatory and external preparations of the present invention usually include various components used in pharmaceuticals, cosmetics, quasi-drugs, veterinary drugs, etc., for example, purified water, ethanol, oily substances, humectants, thickeners, preservatives Agents, emulsifiers, medicinal ingredients, powders, ultraviolet absorbers, pigments, fragrances, emulsion stabilizers, and the like.
- Endothelin (10.3 g) dissolved in phosphate buffered saline (PBS) containing 0.1% bovine serum albumin (BSA) was administered intradermally (50 / L / site) on the back of a guinea pig.
- PBS phosphate buffered saline
- BSA bovine serum albumin
- 0.1 g of each of endothelin-1, endothelin-2, and endothelin-3 dissolved in phosphate buffered saline (PBS) containing 0.1% bovine serum albumin (BSA) is administered intradermally to the back of a shaved guinea pig (50 LZ sites). Three days after administration, the administration site was shaved again, and two days later, the number of elongated hair shafts per fixed area of the administration site was counted. As a result, a significant (p ⁇ 0.01) reduction in the number of hair shafts was observed on the 5th day of administration (Fig. 1).
- Endothelin (10.3 g) dissolved in phosphate buffered saline (PBS) containing 1% bovine serum albumin (BSA) was administered intradermally (50 LZ sites) on the back of guinea pig back. Thereafter, the back skin administered daily was collected, and a tissue specimen was prepared by hematoxylin and eosin staining. As a result, on the first day of administration, a regression image of the growing hair follicle was observed, and on the third day of administration, a transitional hair follicle tissue image was presented (FIG. 2).
- the extent of the bald spots formed 10 days after administration was determined as the integrated value of the major axis and minor axis. Table 1 shows the results. Co-administration of PD 142839 significantly suppressed its formation.
- Hair follicles in the growing stage were surgically isolated from human scalp tissue under a stereoscopic microscope. Organ culture was performed on the isolated hair follicle according to a conventional method (Philpott MP. Et.al. J Cell Sci 97: 463-471, 1990). That is, a William E medium containing L-glutamine (2 mM), Hyde-mouth cortisone (10 ng / mL), insulin (10 zg / mL), sodium selenite (10 ng / mL) and transferrin (10 g / mL). Endothelin-1 was added thereto, and the isolated hair follicles were cultured in the same medium at 37 ° C.
- the components belonging to A are dissolved, and the components belonging to B are separately heated and dissolved.
- B was added to A, mixed uniformly with stirring, emulsified and then cooled to obtain a hair-preserving cream.
- the components belonging to A were uniformly mixed and placed in a container, and B was filled into the container by a conventional method to produce a hair control foam.
- the depilatory agent and the external preparation of the present invention suppress hair growth of mammalian skin, they can be applied to the human body as medicines or cosmetics to enable safe and efficient body hair treatment.
- High quality wool can be harvested when used as a medicine for sheep wool.
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Veterinary Medicine (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Chemical & Material Sciences (AREA)
- Epidemiology (AREA)
- Pharmacology & Pharmacy (AREA)
- Medicinal Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Dermatology (AREA)
- Gastroenterology & Hepatology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Immunology (AREA)
- Zoology (AREA)
- Birds (AREA)
- Organic Chemistry (AREA)
- Marine Sciences & Fisheries (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Dispersion Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Vascular Medicine (AREA)
- Endocrinology (AREA)
- Cosmetics (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Description
Claims
Priority Applications (4)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US10/110,226 US6884772B1 (en) | 1999-10-21 | 2000-10-18 | Depilatories and agents for external use |
EP00969841A EP1222913B1 (en) | 1999-10-21 | 2000-10-18 | Depilating method |
DE60022892T DE60022892T2 (de) | 1999-10-21 | 2000-10-18 | Verfahren zur Enthaarung |
US11/026,090 US7468421B2 (en) | 1999-10-21 | 2005-01-03 | Depilatories and agents for external use |
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
JP29930599 | 1999-10-21 | ||
JP11/299305 | 1999-10-21 |
Related Child Applications (2)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
US10110226 A-371-Of-International | 2000-10-18 | ||
US11/026,090 Continuation US7468421B2 (en) | 1999-10-21 | 2005-01-03 | Depilatories and agents for external use |
Publications (1)
Publication Number | Publication Date |
---|---|
WO2001028509A1 true WO2001028509A1 (fr) | 2001-04-26 |
Family
ID=17870826
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/JP2000/007218 WO2001028509A1 (fr) | 1999-10-21 | 2000-10-18 | Épilatoires et agents pour usage externe |
Country Status (5)
Country | Link |
---|---|
US (2) | US6884772B1 (ja) |
EP (1) | EP1222913B1 (ja) |
JP (2) | JP5043253B2 (ja) |
DE (1) | DE60022892T2 (ja) |
WO (1) | WO2001028509A1 (ja) |
Families Citing this family (10)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
GB0520930D0 (en) * | 2005-10-14 | 2005-11-23 | Reckitt Benckiser Uk Ltd | Composition and method of use |
AU2007313488A1 (en) | 2006-04-04 | 2008-04-24 | Singulex, Inc. | Methods and compositions for highly sensitive analysis of markers and detection of molecules |
US7838250B1 (en) | 2006-04-04 | 2010-11-23 | Singulex, Inc. | Highly sensitive system and methods for analysis of troponin |
US8524690B2 (en) * | 2007-04-26 | 2013-09-03 | The Trustees Of The University Of Pennsylvania | DNA polymerase inhibitors composition and methods |
US8450069B2 (en) | 2009-06-08 | 2013-05-28 | Singulex, Inc. | Highly sensitive biomarker panels |
MX2012009637A (es) * | 2010-02-17 | 2012-09-12 | Procter & Gamble | Articulo depilatorio eficaz. |
WO2011119557A2 (en) * | 2010-03-26 | 2011-09-29 | The Procter & Gamble Company | Kit and method for removing hair |
ES2379703T3 (es) | 2010-03-26 | 2012-04-30 | The Procter And Gamble Company | Método de depilación y kit para depilación |
GB2493540A (en) | 2011-08-10 | 2013-02-13 | Follicum Ab | Agents for stimulating hair growth in mammals |
GB201406989D0 (en) * | 2014-04-17 | 2014-06-04 | Follicum Ab | Novel treatments |
Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4139619A (en) * | 1976-05-24 | 1979-02-13 | The Upjohn Company | 6-Amino-4-(substituted amino)-1,2-dihydro-1-hydroxy-2-iminopyrimidine, topical compositions and process for hair growth |
JPH0499708A (ja) * | 1990-08-13 | 1992-03-31 | Kao Corp | メラニン産生細胞の増殖・色素産生促進剤 |
Family Cites Families (19)
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US2823168A (en) * | 1952-06-06 | 1958-02-11 | Ethicon Inc | Depilatory of thioglycollate salt in gel solution with colloidal excess and method of preparing same |
US3527559A (en) * | 1967-01-05 | 1970-09-08 | Standard Pharmacal Corp | Dense aqueous aerosol foam depilatory compositions containing a mixture of alkaline metal and alkali metal thioglycolates and a fatty alcohol-alkylene oxide wax emulsifying agent |
DE3480171D1 (en) * | 1983-06-20 | 1989-11-23 | Kao Corp | Hair growth promoting composition |
US5519058A (en) * | 1989-03-17 | 1996-05-21 | Cedars-Sinai Medical Center | Method for treatment with dimercaptosuccinic acid (DMSA) of hypertension, diabetic neuphropathy and atherosclerosis |
EP0470256A1 (en) * | 1990-02-26 | 1992-02-12 | Takeda Chemical Industries, Ltd. | Peptide derivatives and production thereof |
JPH072677A (ja) * | 1993-06-18 | 1995-01-06 | Toyo Seito Kk | 経口育毛剤 |
FR2711060B1 (fr) * | 1993-10-13 | 1995-11-17 | Oreal | Procédé pour modifier la pousse des poils et/ou des cheveux et compositions utilisables à cet effet. |
JPH07206843A (ja) * | 1994-01-17 | 1995-08-08 | Kao Corp | 血管拡張剤 |
US5468476A (en) * | 1994-03-16 | 1995-11-21 | Ahluwalia; Gurpreet S. | Reduction of hair growth |
WO1996026712A2 (en) * | 1995-02-28 | 1996-09-06 | Handelman, Joseph, H. | Use of angiogenesis suppressors for inhibiting hair growth |
ATE248004T1 (de) | 1995-06-14 | 2003-09-15 | Hisamitsu Pharmaceutical Co | Schnittstelle für iontophorese |
GB9521101D0 (en) * | 1995-10-16 | 1995-12-20 | Reckitt & Colmann Sa | Improvements in or relating to organic compositions |
US5688499A (en) * | 1996-03-13 | 1997-11-18 | Queen's University At Kingston | Antagonism of endothelin actions |
FR2769225B1 (fr) | 1997-10-03 | 2000-03-10 | Yvon Gauthier | Nouvelles compositions favorisant la pigmentation et leur emploi en dermo-cosmetologie |
JP2001519386A (ja) * | 1997-10-15 | 2001-10-23 | トーマス・ジェファーソン・ユニバーシティ | 哺乳動物における血管収縮または血管痙攣を予防または緩和するための一酸化窒素供与体組成物、方法、装置およびキット |
AU2479099A (en) | 1998-01-28 | 1999-08-16 | Seatrace Pharmaceuticals, Inc. | Topical vasodilatory gel composition and methods of use and production |
US20010056068A1 (en) * | 1998-03-04 | 2001-12-27 | Kristof Chwalisz | Method of treatment and prevention of nitric oxide deficiency-related disorders with citrulline and citrulline derivatives |
US6375948B1 (en) * | 1999-07-12 | 2002-04-23 | Kao Corporation | Treating method for suppressing hair growth |
CN1100526C (zh) * | 1999-11-12 | 2003-02-05 | 上海家化联合股份有限公司 | 一种皮肤美白组合物 |
-
2000
- 2000-10-18 JP JP2000317601A patent/JP5043253B2/ja not_active Expired - Fee Related
- 2000-10-18 WO PCT/JP2000/007218 patent/WO2001028509A1/ja active IP Right Grant
- 2000-10-18 DE DE60022892T patent/DE60022892T2/de not_active Expired - Lifetime
- 2000-10-18 US US10/110,226 patent/US6884772B1/en not_active Expired - Fee Related
- 2000-10-18 EP EP00969841A patent/EP1222913B1/en not_active Expired - Lifetime
-
2005
- 2005-01-03 US US11/026,090 patent/US7468421B2/en not_active Expired - Fee Related
-
2009
- 2009-09-28 JP JP2009222379A patent/JP2009298815A/ja not_active Withdrawn
Patent Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4139619A (en) * | 1976-05-24 | 1979-02-13 | The Upjohn Company | 6-Amino-4-(substituted amino)-1,2-dihydro-1-hydroxy-2-iminopyrimidine, topical compositions and process for hair growth |
JPH0499708A (ja) * | 1990-08-13 | 1992-03-31 | Kao Corp | メラニン産生細胞の増殖・色素産生促進剤 |
Non-Patent Citations (2)
Title |
---|
"The Merck Index", 1996, MERK RESEARCH LABORATORIES DIVISION OF MERCK & CO., INC., XP002936316 * |
See also references of EP1222913A4 * |
Also Published As
Publication number | Publication date |
---|---|
EP1222913A4 (en) | 2003-06-25 |
JP2001187720A (ja) | 2001-07-10 |
US7468421B2 (en) | 2008-12-23 |
JP5043253B2 (ja) | 2012-10-10 |
JP2009298815A (ja) | 2009-12-24 |
DE60022892D1 (de) | 2006-02-09 |
US6884772B1 (en) | 2005-04-26 |
EP1222913A1 (en) | 2002-07-17 |
US20050112079A1 (en) | 2005-05-26 |
DE60022892T2 (de) | 2006-07-13 |
EP1222913B1 (en) | 2005-09-28 |
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