WO2000027795A1 - Transfection reagents - Google Patents
Transfection reagents Download PDFInfo
- Publication number
- WO2000027795A1 WO2000027795A1 PCT/US1999/026825 US9926825W WO0027795A1 WO 2000027795 A1 WO2000027795 A1 WO 2000027795A1 US 9926825 W US9926825 W US 9926825W WO 0027795 A1 WO0027795 A1 WO 0027795A1
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- group
- compound
- alkyl
- independently
- alkenyl
- Prior art date
Links
- 0 *C(C*(*)*)CNC(*)=* Chemical compound *C(C*(*)*)CNC(*)=* 0.000 description 6
- MVSHTDBHDQPDDN-UHFFFAOYSA-N CC1(CCCNC)CCNCCC1 Chemical compound CC1(CCCNC)CCNCCC1 MVSHTDBHDQPDDN-UHFFFAOYSA-N 0.000 description 1
- SMNYJTXSQAQFCJ-UHFFFAOYSA-N CCCCCN(C)CCCC1(C)CCNCCC1 Chemical compound CCCCCN(C)CCCC1(C)CCNCCC1 SMNYJTXSQAQFCJ-UHFFFAOYSA-N 0.000 description 1
- AEUGPBYLZNGABC-HYXAFXHYSA-N CCCCNC/C=C\C Chemical compound CCCCNC/C=C\C AEUGPBYLZNGABC-HYXAFXHYSA-N 0.000 description 1
Classifications
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/13—Amines
- A61K31/14—Quaternary ammonium compounds, e.g. edrophonium, choline
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/14—Particulate form, e.g. powders, Processes for size reducing of pure drugs or the resulting products, Pure drug nanoparticles
- A61K9/16—Agglomerates; Granulates; Microbeadlets ; Microspheres; Pellets; Solid products obtained by spray drying, spray freeze drying, spray congealing,(multiple) emulsion solvent evaporation or extraction
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
- A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- A61K38/17—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- A61K38/22—Hormones
- A61K38/28—Insulins
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
- A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- A61K38/17—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- A61K38/40—Transferrins, e.g. lactoferrins, ovotransferrins
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
- A61K47/50—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates
- A61K47/51—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent
- A61K47/54—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent the modifying agent being an organic compound
- A61K47/543—Lipids, e.g. triglycerides; Polyamines, e.g. spermine or spermidine
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
- C07C211/00—Compounds containing amino groups bound to a carbon skeleton
- C07C211/01—Compounds containing amino groups bound to a carbon skeleton having amino groups bound to acyclic carbon atoms
- C07C211/20—Compounds containing amino groups bound to a carbon skeleton having amino groups bound to acyclic carbon atoms of an acyclic unsaturated carbon skeleton
- C07C211/21—Monoamines
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
- C07C211/00—Compounds containing amino groups bound to a carbon skeleton
- C07C211/62—Quaternary ammonium compounds
- C07C211/63—Quaternary ammonium compounds having quaternised nitrogen atoms bound to acyclic carbon atoms
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
- C07C211/00—Compounds containing amino groups bound to a carbon skeleton
- C07C211/62—Quaternary ammonium compounds
- C07C211/64—Quaternary ammonium compounds having quaternised nitrogen atoms bound to carbon atoms of six-membered aromatic rings
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
- C07C229/00—Compounds containing amino and carboxyl groups bound to the same carbon skeleton
- C07C229/02—Compounds containing amino and carboxyl groups bound to the same carbon skeleton having amino and carboxyl groups bound to acyclic carbon atoms of the same carbon skeleton
- C07C229/04—Compounds containing amino and carboxyl groups bound to the same carbon skeleton having amino and carboxyl groups bound to acyclic carbon atoms of the same carbon skeleton the carbon skeleton being acyclic and saturated
- C07C229/26—Compounds containing amino and carboxyl groups bound to the same carbon skeleton having amino and carboxyl groups bound to acyclic carbon atoms of the same carbon skeleton the carbon skeleton being acyclic and saturated having more than one amino group bound to the carbon skeleton, e.g. lysine
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07D—HETEROCYCLIC COMPOUNDS
- C07D209/00—Heterocyclic compounds containing five-membered rings, condensed with other rings, with one nitrogen atom as the only ring hetero atom
- C07D209/02—Heterocyclic compounds containing five-membered rings, condensed with other rings, with one nitrogen atom as the only ring hetero atom condensed with one carbocyclic ring
- C07D209/44—Iso-indoles; Hydrogenated iso-indoles
- C07D209/48—Iso-indoles; Hydrogenated iso-indoles with oxygen atoms in positions 1 and 3, e.g. phthalimide
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07D—HETEROCYCLIC COMPOUNDS
- C07D241/00—Heterocyclic compounds containing 1,4-diazine or hydrogenated 1,4-diazine rings
- C07D241/02—Heterocyclic compounds containing 1,4-diazine or hydrogenated 1,4-diazine rings not condensed with other rings
- C07D241/10—Heterocyclic compounds containing 1,4-diazine or hydrogenated 1,4-diazine rings not condensed with other rings having three double bonds between ring members or between ring members and non-ring members
- C07D241/12—Heterocyclic compounds containing 1,4-diazine or hydrogenated 1,4-diazine rings not condensed with other rings having three double bonds between ring members or between ring members and non-ring members with only hydrogen atoms, hydrocarbon or substituted hydrocarbon radicals, directly attached to ring carbon atoms
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
- C12N15/88—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation using microencapsulation, e.g. using amphiphile liposome vesicle
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S435/00—Chemistry: molecular biology and microbiology
- Y10S435/81—Packaged device or kit
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Veterinary Medicine (AREA)
- Pharmacology & Pharmacy (AREA)
- Public Health (AREA)
- Epidemiology (AREA)
- Animal Behavior & Ethology (AREA)
- Zoology (AREA)
- Genetics & Genomics (AREA)
- Gastroenterology & Hepatology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Immunology (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Biophysics (AREA)
- Endocrinology (AREA)
- Diabetes (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Plant Pathology (AREA)
- Physics & Mathematics (AREA)
- Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
- Medicinal Preparation (AREA)
- Polyamides (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Acyclic And Carbocyclic Compounds In Medicinal Compositions (AREA)
- Materials For Photolithography (AREA)
- Polysaccharides And Polysaccharide Derivatives (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Plural Heterocyclic Compounds (AREA)
Priority Applications (7)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CA2350882A CA2350882C (en) | 1998-11-12 | 1999-11-12 | Transfection reagents |
NZ512244A NZ512244A (en) | 1998-11-12 | 1999-11-12 | Polycationic transfection reagents for introducing anions into a cell |
AU14776/00A AU772847B2 (en) | 1998-11-12 | 1999-11-12 | Transfection reagents |
EP99971794A EP1129064B1 (en) | 1998-11-12 | 1999-11-12 | Transfection reagents |
DK99971794T DK1129064T3 (da) | 1998-11-12 | 1999-11-12 | Transfektionsreagenser |
DE69937964T DE69937964T2 (de) | 1998-11-12 | 1999-11-12 | Transportreagentien |
JP2000580975A JP4854853B2 (ja) | 1998-11-12 | 1999-11-12 | トランスフェクション薬剤 |
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US10811798P | 1998-11-12 | 1998-11-12 | |
US60/108,117 | 1998-11-12 |
Publications (1)
Publication Number | Publication Date |
---|---|
WO2000027795A1 true WO2000027795A1 (en) | 2000-05-18 |
Family
ID=22320415
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/US1999/026825 WO2000027795A1 (en) | 1998-11-12 | 1999-11-12 | Transfection reagents |
Country Status (13)
Cited By (53)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2000077032A2 (en) * | 1999-06-16 | 2000-12-21 | Smithkline Beecham P.L.C. | Spermine:peptide-based surfactant compounds |
WO2002077202A1 (en) | 2001-03-27 | 2002-10-03 | Invitrogen Corporation | Culture medium for cell growth and transfection |
WO2003082809A1 (en) * | 2002-03-27 | 2003-10-09 | Glaxo Group Limited | Diaminoacid-aminoacid-polyamine based gemini surfactant compounds |
US6696424B1 (en) | 1999-05-28 | 2004-02-24 | Vical Incorporated | Cytofectin dimers and methods of use thereof |
US6716882B2 (en) | 1993-12-20 | 2004-04-06 | Invitrogen Corporation | Highly packed polycationic ammonium, sulfonium and phosphonium lipids |
WO2004035523A1 (en) * | 2002-10-16 | 2004-04-29 | Neopharm, Inc. | Cationic cardiolipin analogs and use thereof |
US6989434B1 (en) | 1994-02-11 | 2006-01-24 | Invitrogen Corporation | Reagents for intracellular delivery of macromolecules |
WO2007046356A1 (ja) * | 2005-10-18 | 2007-04-26 | Otsuka Pharmaceutical Co., Ltd. | 核酸送達用キャリアー組成物 |
US7294511B2 (en) | 2001-03-22 | 2007-11-13 | Chromos Molecular Systems, Inc. | Methods for delivering nucleic acid molecules into cells and assessment thereof |
US7531693B2 (en) | 2003-05-22 | 2009-05-12 | Molecular Transfer, Inc. | Lipids for transfection of nucleic acids |
EP2097526A4 (en) * | 2006-11-17 | 2010-11-03 | Univ California | SYSTEM FOR EXPRESSING A NELL PEPTIDE |
EP1986696B1 (de) * | 2006-02-23 | 2012-01-11 | Qiagen GmbH | Ein verfahren zur herstellung einer transformierten zelle |
WO2012068176A1 (en) | 2010-11-15 | 2012-05-24 | Life Technologies Corporation | Amine-containing transfection reagents and methods for making and using same |
US8338584B2 (en) | 2007-11-08 | 2012-12-25 | Otsuka Pharmaceutical Co., Ltd. | Nucleic acid complex and nucleic acid delivery composition |
EP2604255A1 (en) | 2006-05-05 | 2013-06-19 | Molecular Transfer, Inc. | Novel reagents for transfection of eukaryotic cells |
WO2013166339A1 (en) | 2012-05-02 | 2013-11-07 | Life Technologies Corporation | High yield transient expression in mammalian cells using unique pairing of high density growth and transfection medium and expression enhancers |
US9090912B1 (en) | 2009-03-04 | 2015-07-28 | Hirofumi Takeuchi | Nucleic acid complex and nucleic acid-delivering composition |
CN104876831A (zh) * | 2015-04-03 | 2015-09-02 | 苏州圣诺生物医药技术有限公司 | 脂质修饰精胺衍生物及利用该衍生物制备的脂质体 |
EP2951292A2 (en) * | 2013-01-30 | 2015-12-09 | Paolo Gresele | Platelets transfected by exogenous genetic material and platelet microparticles obtained by said transfected platelets, method for the preparation and uses thereof |
WO2016011203A1 (en) | 2014-07-15 | 2016-01-21 | Life Technologies Corporation | Compositions with lipid aggregates and methods for efficient delivery of molecules to cells |
US9358300B2 (en) | 1998-11-12 | 2016-06-07 | Life Technologies Corporation | Transfection reagents |
WO2016112963A1 (en) | 2015-01-13 | 2016-07-21 | Riboxx Gmbh | Delivery of biomolecules into cells |
WO2017011598A1 (en) | 2015-07-13 | 2017-01-19 | Life Technologies Corporation | System and method for improved transient protein expression in cho cells |
WO2017040335A2 (en) | 2015-08-28 | 2017-03-09 | Molecular Transfer, Inc. | Transfection complexes and methods of using the same |
US9708628B2 (en) | 2011-11-18 | 2017-07-18 | Nof Corporation | Cationic lipid having improved intracellular kinetics |
CN107406396A (zh) * | 2015-01-30 | 2017-11-28 | 日油株式会社 | 阳离子性脂质 |
WO2018035377A1 (en) | 2016-08-17 | 2018-02-22 | Factor Bioscience Inc. | Nucleic acid products and methods of administration thereof |
WO2019045897A1 (en) * | 2017-08-31 | 2019-03-07 | Life Technologies Corporation | CATIONIC LIPIDIC COMPOSITIONS FOR TISSUE-SPECIFIC ADMINISTRATION |
WO2019128611A1 (en) * | 2017-12-29 | 2019-07-04 | Suzhou Ribo Life Science Co., Ltd. | Conjugates and preparation and use thereof |
EP3543339A1 (en) | 2015-02-13 | 2019-09-25 | Factor Bioscience Inc. | Nucleic acid products and methods of administration thereof |
EP3542825A1 (en) * | 2014-11-10 | 2019-09-25 | Ethris GmbH | Induction of osteogenesis by delivering bmp encoding rna |
CN110506035A (zh) * | 2018-03-16 | 2019-11-26 | 株式会社东芝 | 生物降解性化合物、脂质粒子、含有脂质粒子的组合物、和试剂盒 |
US10501404B1 (en) | 2019-07-30 | 2019-12-10 | Factor Bioscience Inc. | Cationic lipids and transfection methods |
US10538784B2 (en) | 2016-03-01 | 2020-01-21 | Molecular Transfer, Inc. | Plant virus movement proteins and methods of using the same |
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US10829787B2 (en) | 2015-10-14 | 2020-11-10 | Life Technologies Corporation | Ribonucleoprotein transfection agents |
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US11236334B2 (en) | 2017-08-22 | 2022-02-01 | National University Corporation Nagoya University | Modified polynucleotide |
EP4015634A1 (en) | 2020-12-15 | 2022-06-22 | Sylentis, S.A.U. | Sirna and compositions for prophylactic and therapeutic treatment of virus diseases |
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US11492620B2 (en) | 2017-12-01 | 2022-11-08 | Suzhou Ribo Life Science Co., Ltd. | Double-stranded oligonucleotide, composition and conjugate comprising double-stranded oligonucleotide, preparation method thereof and use thereof |
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US5795587A (en) * | 1995-01-23 | 1998-08-18 | University Of Pittsburgh | Stable lipid-comprising drug delivery complexes and methods for their production |
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