Classifications

• • C—CHEMISTRY; METALLURGY
  • C07—ORGANIC CHEMISTRY
  • C07F—ACYCLIC, CARBOCYCLIC OR HETEROCYCLIC COMPOUNDS CONTAINING ELEMENTS OTHER THAN CARBON, HYDROGEN, HALOGEN, OXYGEN, NITROGEN, SULFUR, SELENIUM OR TELLURIUM
  • C07F9/00—Compounds containing elements of Groups 5 or 15 of the Periodic Table
  • C07F9/02—Phosphorus compounds
  • C07F9/28—Phosphorus compounds with one or more P—C bonds
  • C07F9/38—Phosphonic acids [RP(=O)(OH)2]; Thiophosphonic acids ; [RP(=X1)(X2H)2(X1, X2 are each independently O, S or Se)]
• • C—CHEMISTRY; METALLURGY
  • C07—ORGANIC CHEMISTRY
  • C07F—ACYCLIC, CARBOCYCLIC OR HETEROCYCLIC COMPOUNDS CONTAINING ELEMENTS OTHER THAN CARBON, HYDROGEN, HALOGEN, OXYGEN, NITROGEN, SULFUR, SELENIUM OR TELLURIUM
  • C07F9/00—Compounds containing elements of Groups 5 or 15 of the Periodic Table
  • C07F9/02—Phosphorus compounds
  • C07F9/28—Phosphorus compounds with one or more P—C bonds
  • C07F9/38—Phosphonic acids [RP(=O)(OH)2]; Thiophosphonic acids ; [RP(=X1)(X2H)2(X1, X2 are each independently O, S or Se)]
  • C07F9/3804—Phosphonic acids [RP(=O)(OH)2]; Thiophosphonic acids ; [RP(=X1)(X2H)2(X1, X2 are each independently O, S or Se)] not used, see subgroups
  • C07F9/3839—Polyphosphonic acids
  • C07F9/3873—Polyphosphonic acids containing nitrogen substituent, e.g. N.....H or N-hydrocarbon group which can be substituted by halogen or nitro(so), N.....O, N.....S, N.....C(=X)- (X =O, S), N.....N, N...C(=X)...N (X =O, S)
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
  • A61P19/00—Drugs for skeletal disorders
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
  • A61P19/00—Drugs for skeletal disorders
  • A61P19/08—Drugs for skeletal disorders for bone diseases, e.g. rachitism, Paget's disease
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
  • A61P19/00—Drugs for skeletal disorders
  • A61P19/08—Drugs for skeletal disorders for bone diseases, e.g. rachitism, Paget's disease
  • A61P19/10—Drugs for skeletal disorders for bone diseases, e.g. rachitism, Paget's disease for osteoporosis

Definitions

• This invention relates to new hydrate and crystalline forms of alendronate sodium, processes for the manufacture thereof, and pharmaceutical compositions thereof.
• Alendronate sodium the sodium salt of alendronic acid, also known as 4-amino- 1 -hydroxybutylidene-l , l-bisphosphonic acid monosodium, has the formula I:
• alendronic acid Various methods for preparing alendronic acid are known in the art and have been disclosed in M.I. Kabachnik et al., Synthesis and Acid - Base and Complexing Properties of Amino-Substituted ⁇ -Hydroxyalkylidene-diphosphonic Acids, Izv. Akad.
• U.S. Patent No. 4,922,007 describes the preparation of a trihydrate of alendronate sodium by reaction of 4-aminobutyric acid with phosphorous acid and phosphorous trichloride in the presence of methanesulfonic acid followed by the addition of sodium hydroxide.
• the present invention provides new hydrate forms of alendronate sodium, having water content of 1.3 to 1 1.7 percent, and processes for their manufacture. Moreover, the present invention provides new crystalline forms of alendronate sodium, designated forms B, D, E, F, G and H, and processes for the manufacture thereof.
• the present invention provides novel hydrate forms of alendronate sodium having water content of between 1. 3 and 11.7 percent water.
• the present invention relates to the following novel hydrate forms forms of alendronate monosodium: 1/4 hydrate, 1/3 hydrate, hemihydrate, 2/3 hydrate, 3/4 hydrate, monohydrate, 5/4 hydrate. 4/3 hydrate, 3/2 hydrate, 5/3 hydrate, 7/4 hydrate and dehydrate.
• the present invention provides a new crystalline Form B of alendronate sodium, having a powder x-ray diffractogram substantially as depicted in Fig.
• Alendronate sodium Form B has significant IR bands as depicted in Fig. lb at 654 cm '1 , 955 cm “1 , 1074 cm- 1, 1261 cm “1 , 1309 cm “1 , and 1614 cm “1 .
• Another embodiment of the invention is a new crystalline Form D of alendronate sodium, having a powder X-ray diffractogram substantially as depicted in Fig. 4a, with characteristic peaks at 13.1 ⁇ 0.2, 15.2 ⁇ 0.2, 16.3 ⁇ 0.2, 18.4 ⁇ 0.2, 20.8 ⁇ 0.2, 22.3 ⁇ 0.2, 22.5 ⁇ 0.2, 23.4 ⁇ 0.2, 23.7 ⁇ 0.2, 31.4 ⁇ 0.2, and 35.7 ⁇ 0.2 degrees 2 theta.
• Form D as depicted in Fig.
• An additional embodiment is a new crystalline Form E of alendronate sodium, having a powder X-ray diffractogram substantially as depicted in FIG. 5a, with characteristic peaks at 7.0 ⁇ 0.2, 9.3 ⁇ 0.2, 11.8 ⁇ 0.2, 13.3 ⁇ 0.2, 14.0 ⁇ 0.2, 15.3 ⁇ 0.2, 16.2 ⁇ 0.2, 17.4 ⁇ 0.2, and 19.4 ⁇ 0.2 degrees 2 theta.
• Form E has significant IR bands as depicted in Fig.
• a still further embodiment of the invention is a new crystalline Form F of alendronate sodium, having a powder X-ray diffractogram substantially as depicted in
• FIG. 6a with characteristic peaks at 9.3 ⁇ 0.2, 1 1.7 ⁇ 0.2, 13.0 ⁇ 0.2,13.4 ⁇ 0.2, 14.2 ⁇ 0.2,
• Form F has significant IR bands as depicted in Fig. 6b at 660 cm “1 , 893 cm “1 , 930 cm “1 , 9953 cm “ ', 970 cm “1 , 982 cm “ ', 1010 cm “ ', 1033 cm '1 1052 cm “ ', 1060 cm “1 , 1069 cm “1 , 1 109 cm “1 and 1 169 cm “1 , 1251 cm “ ', 1338 cm “ ', 1498 cm “ ', 1544 cm ', 1603 cm '1 , 1637 cm '1 , 1664 cm “ '.
• the TGA Fig. 5c curve shows a gradual loss on drying of 4.1 % up to 150°C.
• a further embodiment is a new crystalline Form G of alendronate sodium, having a powder X-ray diffractogram substantially as depicted in FIG. 7a, with characteristic peaks at 9.5 ⁇ 0.2, 10.1 ⁇ 0.2, 12.7 ⁇ 0.2, 16.2 ⁇ 0.2, 17.3 ⁇ 0.2, 17.6 ⁇ 0.2, 19.1 ⁇ 0.2, 20.4 ⁇ 0.2, 20.9 ⁇ 0.2, 22.1 ⁇ 0.2, 24.8 ⁇ 0.2, 25.5 ⁇ 0.2, 28.0 ⁇ 0.2, 29.0 ⁇ 0.2, 29.6 ⁇ 0.2,
• Form G has significant IR bands as depicted in Fig. 7b at 665 cm” ', 751 cm “ ', 856 cm “ ', 895 cm “ ', 913 cm “ ', 939 cm “ ', 101 1 cm “ ', 1021 cm “ ', 1050 cm “1 , 1091 cm “1 , 1 155 cm '1 , 1273 cm “1 , 1305 cm “ ', 1337 cm “1 , 1510 cm “1 , and 1639 cm “ ' .
• Yet another embodiment is a new crystalline Form H of alendronate sodium, having a powder X-ray diffractogram substantially as depicted in FIG. 8a, with characteristic peaks at 9.2 ⁇ 0.2, 13.0 ⁇ 0.2, 14.2 ⁇ 0.2, 15.0 ⁇ 0.2, 17.1 ⁇ 0.2, 20.7 ⁇ 0.2, 22.0 ⁇ 0.2, 22.4 ⁇ 0.2, degrees two theta.
• Form H has significant IR bands, as depicted in Fig.
• All of sodium alendronate crystalline forms B, D, E, F, G and H contain water in the amount of 2.2 to 9.0%> by weight.
• the invention further provides a new hydrate form of alendronate sodium having a water content of 1.3 % to 3.1 %.
• a further embodiment is a new hydrate form of alendronate sodium having a water content of 2.5% to 3.5%.
• a further embodiment is a new hydrate form of alendronate sodium having a water content of 2.8% to 3.9%.
• An additional embodiment is a new hydrate form of alendronate sodium having a water content of 3.2% to 5.8%o.
• Another embodiment is a new hydrate form of alendronate sodium having a water content of 5.1 % to 7. 0%.
• a still further embodiment is a new hydrate form of alendronate sodium having a water content of 6.4%) to 9.0%.
• the invention also provides a new crystalline Form B of alendronate sodium, having a water content of 6.4% to 9.0%>.
• the invention further provides a new crystalline Form D of alendronate sodium, having a water content of 3.2% to 5.8%.
• the invention further provides a new crystalline Form F of alendronate sodium, having a water content of 1.3 % to 3.1 %.
• the invention further provides a new crystalline Form G of alendronate sodium, having a water content of 5.1 % to 7.0%.
• the invention further provides a new crystalline Form E of alendronate sodium, having a water content of 2.8% to 3.9%o.
• the invention further provides a new crystalline Form H of alendronate sodium, having a water content of 2.5% to 3.5%.
• the invention provides a new monohydrate and a new dehydrate of alendronate sodium, having an X-ray diffractogram substantially as depicted in FIG. 2a and 3a, accordingly, with characteristic peaks at 9.3 ⁇ 0.2, 12.4 ⁇ 0.2, 13.5 ⁇ 0.2,17.1 ⁇ 0.2, 18.5 ⁇ 0.2, 19.7 ⁇ 0.2, 20.3 ⁇ 0.2, 21.0 ⁇ 0.2, 21.8 ⁇ 0.2, 23.4 ⁇ 0.2, 24.3 ⁇ 0.2, 24.9 ⁇ 0.2, 26.3 ⁇ 0.2, 30.0 ⁇ 0.2, and 34.4 ⁇ 0.2 degrees 2 theta.
• Form C as depicted in Figs.
• 2b and 3b has significant IR bands at 660 cm “1 , 745 cm “ ', 865 cm-1, 913 cm “1 , 952 cm “1 , 966 cm “1 , 1017 cm '1 , 1046 cm “1 , 1 128 cm “1 , 1 174 cm “ ', 1235 cm “1 , 1340 cm “1 , 1402 cm “1 , 1544 cm “1 ,
• the TGA curve of the monohydrate Form C shows a loss on drying of 5.6%> which implies that the crystal Form C contains a stoichiometric quantity of water close to that of the monohydrate (expected loss on drying value: 6.2%).
• the TGA curve of the dehydrate Form C shows a sharp loss on drying of 12.0% which implies that the crystal Form C contains a stoichiometric quantity of water corresponding to dehydrate (expected loss on drying value: 1 1.7%).
• the present invention also relates to the method of preparing the compound 4-amino-l-hydroxybutylidene-l , 1-bisphosphonic acid monosodium salt having water content of 1.3% to 1 1.7% by reacting alendronic acid with one equivalent of sodium base in an aqueous organic solvent selected from the group consisting of. acetone, DMSO,
• the invention further provides a method for making Form D of alendronate sodium, comprising treating alendronic acid anhydrous in a lower alkanol with 1 equivalent of sodium base and 0 to 4 equivalents of water, followed by isolating the crystalline alendronate sodium Form D.
• the invention further provides a method for making Form E of alendronate sodium, comprising treating alendronic acid, which is in anhydrous or monohydrate form, in a lower alkanol with I equivalent of sodium base and 9 to 15 equivalents of water, followed by isolating the crystalline alendronate sodium Form E.
• the invention further provides a method for making Form F of alendronate sodium, comprising treating alendronic acid, in a lower alkanol with 1 equivalent of sodium base and 5 to 8 equivalents of water for anhydrous form and 3 to 20 equivalents of water for monohydrate form, followed by isolating the crystalline alendronate sodium Form F.
• the invention further provides a method for making alendronate sodium monohydrate. comprising treating alendronic acid, in a lower alkanol with 1 equivalent of sodium base and water under the conditions described hereinafter, followed by isolating the alendronate sodium monohydrate
• the invention further provides a method for making Form G of alendronate sodium, comprising treating alendronic acid, in a lower alkanol with 1 equivalent of sodium base and water under the conditions described hereinafter, followed by isolating the crystalline alendronate sodium Form G.
• the invention further provides a method for making Form G of alendronate sodium comprising treating any one or more of the crystal forms of alendronate sodium selected from the group which consists of Form B, Form C, Form D, Form E, Form F and Form H, in a lower alkanol, preferably ethanol, with 20-40 equivalents of water under the conditions described hereinafter followed by isolating the crystalline alendronate sodium Form G.
• the invention further provides a method for making Form G of alendronate sodium comprising treating alendronate monosodium trihydrate in a lower alkanol, preferably ethanol, with 25-35 equivalents of water under the condition described hereinafter, followed by isolating the crystalline alendronate sodium Form G.
• the invention further provides a method for making Form G of alendronate sodium comprising treating any one or more forms of alendronate sodium salts preferably selected from the group consisting of monosodium, disodium, trisodium and tetrasodium salts, in a lower alkanol preferably ethanol with 20-40 equivalents of water under the conditions described hereinafter, followed by isolating the crystalline alendronate sodium Form G.
• the starting sodium salt is higher than monosodium (e.g. disodium, trisodium or tetrasodium) it is necessary to add an acid, preferably alendronic acid, in order to maintain the pH at about 4.4.
• the invention further provides a method for making Form H of alendronate sodium, comprising treating alendronic acid, which is the anhydrous or monohydrate form, in a lower alkanol with one equivalent of sodium base and 25 to 35 equivalents of water, under the conditions described hereinafter, followed by isolating the crystalline alendronate sodium Form H.
• the invention further provides a method for making Form B of alendronate sodium, comprising treating alendronic acid monohydrate in a lower alkanol with one equivalent of sodium base and 0 to 4 equivalents of water, followed by obtaining the crystalline alendronate sodium Form B.
• the invention further provides a method for making alendronate sodium dehydrate comprising treating crystalline alendronate sodium trihydrate with an effective amount of drying agent followed by isolating the crystalline alendronate sodium dehydrate.
• the invention further provides a method for making alendronate sodium monohydrate comprising treating crystalline alendronate sodium trihydrate with a sufficient amount of drying agent followed by isolating the crystalline alendronate sodium monohydrate.
• the invention further provides a method for making alendronate sodium monohydrate comprising treating crystalline alendronate sodium dehydrate with a sufficient amount of drying agent followed by isolating the crystalline alendronate sodium monohydrate.
• the invention further relates to a pharmaceutical composition which comprises alendronate sodium, having water content of 1.3 to 1 1.7 percent in a therapeutically effective amount, and a pharmaceutically acceptable carrier.
• the invention further relates to a pharmaceutical composition which comprises alendronate sodium in Form B, D, E, F, G and/or H in a therapeutically effective amount, and a pharmaceutically acceptable carrier.
• FIGS, la, lb, and lc show, respectively, the powder X-ray diffraction spectmm, the thermograviometric (TGA) curve and the infrared spectrum of alendronate sodium Form B.
• FIGS. 2a, 2b, and 2c show, respectively, the powder X-ray diffraction spectmm, the thermograviometric (TGA) curve and the infrared spectrum of alendronate sodium monohydrate Form C.
• FIGS. 3a, 3b, and 3c show, respectively, the powder X-ray diffraction spectrum, the thermograviometric (TGA) curve and the infrared spectrum of alendronate sodium dehydrate Form C.
• FIGS. 4a, 4b, and 4c show, respectively, the powder X-ray diffraction spectmm, the thermograviometric (TGA) curve and the infrared spectmm of alendronate sodium Form D.
• FIGS. 5a, 5b, and 5c show, respectively, the powder X-ray diffraction spectmm, the (thermograviometric (TGA) curve) and the infrared spectmm of alendronate sodium Form E.
• FIGS. 6a, 6b, and 6c show, respectively, the powder X-ray diffraction spectmm, the thermograviometric (TGA) curve and the infrared spectrum of alendronate sodium Form F.
• FIGS. 7a, 7b, and 7c show, respectively, the powder X-ray diffraction spectmm, the then-nograviometric (TGA) curve and the infrared spectmm of alendronate sodium Form G.
• Figures 8a, 8b and 8c show respectively the powder X-ray diffraction spectmm, the thermograviometric (TGA) curve and the infrared spectmm of alendronate sodium of Form H.
• the invention discloses new hydrate forms of alendronate sodium having water contents of 1. 3 percent to 1 1.7 percent.
• the present invention also discloses new crystalline forms of alendronate sodium which have been designated Forms B, D, E, F, G and H.
• water content refers to the content of water based upon the Loss on Drying method as described in Pharmacopeial Fomm, Vol. 24, No. 1, page 5438 (Jan - Feb 1998). The calculation of water content is based upon the percent of weight that is lost by drying. For Forms G and H the term “water content” refers to the content of water based upon a TGA measurement and a step analysis in the temperature range of about
• lower alkanol refers to alkanols having 1 to 4 carbon atoms.
• Preferred lower alkanols include ethanol, methanol and isopropanol.
• equivalents of water means molar equivalents of water.
• equivalents of sodium base means molar equivalents of sodium base.
• hemihydrate when used in reference to the monosodium salt of alendronic acid describes a crystalline material having a water content of approximately 3.2%.
• 2/3 hydrate when used in reference to the monosodium salt of alendronic acid describes a crystalline material having a water content of approximately 4.2%.
• sodium base refers to sodium hydroxide and the sodium alkoxide of a lower alkanol.
• Alendronic acid can be prepared by methods that are well known in the art.
• Alendronic acid can also be prepared by the process disclosed in U.S. Patent No. 4,621,077. It will be appreciated that when alendronic acid is recrystallized from water, as in the above process, the monohydrate is formed.
• Alendronate sodium trihydrate can be prepared by the process disclosed in U.S. Patent No. 4,922,007.
• Alendronic acid monohydrate can be converted to alendronic acid anhydrous by heating in a vacuum oven at 1 10-220°C at a vacuum of less than 5 mm Hg for 24 hours.
• alendronic acid anhydrous as prepared by any of the known methods is added to a lower alkanol, preferably ethanol, together with a sodium base, preferably sodium hydroxide, and an amount of water that depends upon the desired crystal form of alendronate sodium.
• a sodium base preferably sodium hydroxide
• the molar ratio of sodium base to alendronic acid is 1 : 1.
• Those skilled in the art will appreciate that a higher ratio of NaOH would yield the undesirable disodium and trisodium salts.
• the reaction mixture is boiled under reflux while being stirred vigorously for approximately 15 hours, until the pH of the liquid phase remains constant (approx. pH 7).
• Crystalline alendronate sodium is then isolated, preferably by filtration after cooling to ambient temperature, washing with absolute ethanol, optionally washing with absolute ethyl ether and drying overnight in a vacuum oven at ambient temperature and at a pressure of 10 mm to 15 mm of mercury.
• ambient temperature is from about 20°C to about 25 °C.
• alendronic acid monohydrate is converted to alendronate sodium
• alendronic acid monohydrate as prepared by any of the known methods is added to an alkanol, preferably ethanol, together with a sodium base, preferably sodium hydroxide, and a desired amount of water.
• the amount of water depends upon the crystal form that is desired.
• the molar ratio of sodium base to alendronic acid is 1 : 1.
• the reaction mixture is boiled under reflux while stirring vigorously for approximately 15 hours, until the pH of the liquid phase remains constant
• alendronate sodium trihydrate (Form C) is converted to alendronate sodium dehydrate (Form C)
• alendronate sodium trihydrate as prepared by methods known in the art is added to an alkanol which is substantially free of water, preferably absolute ethanol.
• This mixture is treated with a drying agent, preferably by refluxing the mixture in a reflux condenser wherein the condensate formed passes through 3A molecular sieves.
• the weight:weight ratio of molecular sieves to alendronate sodium trihydrate is preferably about 2: 1 and most preferably 12:5. Refluxing of the mixture is preferably done for 24 hours with stirring. Alendronate sodium dehydrate is then isolated, preferably by filtration after cooling to ambient temperature, washing with absolute ether and drying overnight in a vacuum oven at ambient temperature and at a pressure of 10 mm to 15 mm of mercury.
• alendronate sodium trihydrate (Form C) is converted to alendronate sodium monohydrate (Form C)
• alendronate sodium trihydrate as prepared by any of the methods known in the art is added to an alkanol which is substantially free of water, preferably absolute ethanol.
• This mixture is treated with a drying agent, preferably by refluxing the mixture in a reflux condenser wherein the condensate formed passed through 3A molecular sieves. If and when a first portion of molecular sieves is exhausted, a second portion of fresh molecular sieves is used.
• the weight: weight ratio of molecular sieves to alendronate sodium trihydrate is preferably about 2: 1 and most preferably 12:5. Refluxing of the mixture is preferably done for 24 hours with stirring. The mixture is allowed to cool to ambient temperature before recharging with an equivalent amount of molecular sieves.
• Alendronate sodium monohydrate is then isolated, preferably by cooling to ambient temperature, filtration, washing with absolute ether and drying overnight in a vacuum oven at ambient temperature and a pressure of 10 mm and 15 mm of mercury.
• the new crystalline forms of alendronate sodium and the new hydrate forms of alendronate sodium may be prepared as pharmaceutical compositions which are particularly useful for the treatment of bone reso ⁇ tion in bone diseases including osteoporosis and Paget's disease.
• Such compositions may comprise one of the new crystalline and hydrate forms of alendronate sodium with pharmaceutically acceptable carriers and/or excipients.
• these compositions may be prepared as medicaments to be administered orally, parenterally, rectally, transdermally, bucally, or nasally.
• Suitable forms for oral administration include tablets, compressed or coated pills, dragees, sachets, hard or gelatin capsules, sub-lingual tablets, syrups and suspensions; for parenteral administration the invention provides ampoules or vials that include an aqueous or non- aqueous solution or emulsion; for rectal administration there are provided suppositories with hydrophilic or hydrophobic vehicles; and for topical application as ointments or aerosol formulations known in the art; transdermal delivery there are provided suitable delivery systems as known in the art; and for nasal delivery there are provided suitable aerosol delivery systems known in the art.
• the powder X-ray diffraction patterns were obtained by methods known in the art using a Philips X-Ray powder diffractorneter, Goniometer model 1050/70 at a scanning speed of 2° per minute.
• thermogravimetric curves were obtained by methods known in the art using a Mettler TGA TG50.
• the weight of the samples was about 10 mg.
• the temperature range was from 25 °C to at least 200°C, at the rate of 10°C/min.
• Samples were purged with mitrogen gas at a flow rate of 40 ml/min. Standard 150 ml aluminum crucibles were used.
• the infrared spectra were obtained by methods known in the art using a Perkin Elmer FT-IR Paragon 1000 spectrometer. Samples were analyzed in Nujol mulls. Spectra were obtained at 4 cm " ' resolution and 16 scans each.
• the atomic abso ⁇ tion analysis was obtained by methods known in the art using a Perkin Elmer 5000 Flame Atomic Abso ⁇ tion instrument. Sodium content was determined against standard solutions obtained from Merck and Aldrich.
• Alendronic acid was crystallized from water to make alendronic acid monohydrate.
• the resulting alendronic acid monohydrate was dried at 5O°C at 10 mm Hg pressure for 15 hours to give dry alendronic acid monohydrate containing 6.9% water.
• the alendronic acid monohydrate from Example 1 was further dried at 110- 120°C in 1 mm Hg for 4 hours to give anhydrous alendronic acid.
• the water content was 0.3%) by weight.
• a 250 ml flask was fitted with a mechanical stirrer, a thermometer, and a reflux condenser.
• the flask was charged with 41.1 ml of a solution of sodium hydroxide in ethanol (0.49N, 20.1 mmol), 8.9 ml of ethanol, water (0 to 40 mol. eq., according to the crystal form desired), and 5g (20.1 mmol) of anhydrous alendronic acid.
• the reaction mixture was boiled with vigorous stirring for about 15 hours until the of pH of the liquid phase remained constant (approx. pH 7).
• a 250 ml flask was fitted with a mechanical stirrer, a thermometer, and a reflux condenser.
• the flask was charged with 38.2 ml of a solution of sodium hydroxide in ethanol (0.49 N, 18.7 mmol), 4.8 ml of ethanol, water (0 to 100 mol. eq., according to the crystal form desired), and 5 g (18.7 mmol) of alendronic acid monohydrate.
• the reaction mixture was boiled with vigorous stirring for about 15 hours until stability of pH of the liquid phase was reached (approx. pH 7).
• crystalline Form F when 3-5 mol. eq. water were used
• crystalline Form E when 1 1-13 (preferably 12) mol. eq. water were used
• crystalline Form G when 15-100 (preferably 20-80) mol. eq. water were used.
• the monosodium salt was confirmed by atomic abso ⁇ tion and by measuring the pH of a 0.5%> aqueous solution of the salt (approx. pH 4.4).
• the water content is determined using the TGA technique, heating the sample to 230 °C and calculating the sha ⁇ LOD (loss on drying) step, which occurs above 150°C.
• a one liter flask was fitted with a magnetic bar stirrer, Soxhlet extraction funnel (operating volume 150 ml) charged with 3A molecular sieves (60 g), and reflux condenser connected to a drying tube with 3A molecular sieves.
• the flask was charged with sodium alendronate trihydrate (25 g) and absolute ethanol (450 ml, vol.%> of water ⁇
• Example 6 Preparation of Sodium Alendronate Monohydrate
• a one liter flask was fitted with a magnetic bar stirrer, Soxhlet extraction funnel (operating volume 150 ml) charged with 3 A molecular sieves (60 g), and reflux condenser connected to a drying tube with 3 A molecular sieves.
• the flask was charged with sodium alendronate trihydrate (25 g) and absolute ethanol (450 ml, vol.% of water ⁇ 0.1%).
• the mixture was boiled with stirring for 24 hours.
• the used molecular sieves were replaced by a new portion of 3 A molecular sieves (60 g) and the reflux was continued for additional 24 hours.
• the solid material was filtered, washed with absolute ethyl ether, and dried overnight in a vacuum oven ( 10-15 mm Hg, ambient temperature) to give sodium alendronate monohydrate.
• a one liter flask was fitted with a mechanical stirrer, a thermometer, and a reflux condenser.
• the flask was charged with alendronic acid monohydrate (25 g, 0.094 mol) and aqueous ethanol.
• the mixture was heated to boiling with stirring.
• the aqueous ethanolic sodium hydroxide was added dropwise to the suspension of alendronic acid monohydrate in aqueous ethanol for 3 hours at reflux with vigorously stirring. Then the mixture was stirred at reflux for additional 15 hours.
• the mixture was cooled to room temperature with stirring.
• the solid was filtered, washed with absolute ethanol, then with absolute ethyl ether, and dried overnight in a vacuum oven (10-15 mm Hg, ambient temperature) to give 26.2 g of alendronate sodium, having crystalline Form G.
• a one liter flask was fitted with a mechanical stirrer, a thermometer, and a reflux condenser.
• the flask was charged with alendronic acid monohydrate (25 g, 0.094 mol) and aqueous ethanol.
• the mixture was heated to boiling with stirring.
• the aqueous ethanolic sodium hydroxide was added dropwise to the suspension of alendronic acid monohydrate in aqueous ethanol for 3 hours at reflux with vigorously stirring. Then the mixture was stirred at reflux for additional 15 hours.
• the mixture was cooled to room temperature with stirring.
• the solid was filtered, washed with absolute ethanol, and dried overnight in a vacuum oven (10-15 mm Hg, ambient temperature) to give 26.2 g of alendronate sodium, having crystalline Form G.
• a one liter flask was fitted with a mechanical stirrer, a thermometer, and a reflux, condenser.
• the flask was charged with alendronic acid monohydrate (25 g, 0.094 mol) and aqueous ethanol.
• the mixture was heated to boiling with stirring.
• the aqueous ethanolic sodium hydroxide was added dropwise to the suspension of alendronic acid monohydrate in aqueous ethanol for 3 hours at reflux with vigorously stirring. Then the mixture was stirred at reflux for additional 15 hours.
• the mixture was cooled to room temperature with stirring.
• the solid was filtered, washed with absolute ethanol, and dried overnight in a vacuum oven (10-15 mm Hg, 40-50 °C) to give 26.2g of alendronate sodium, having crystalline Form G.
• a one liter flask was fitted with a mechanical stirrer, a thermometer, and a reflux condenser.
• the flask was charged with alendronic acid monohydrate (25 g, 0.094 mol) and aqueous ethanol.
• the mixture was heated to boiling with stirring.
• the aqueous ethanolic sodium hydroxide was added dropwise to the suspension of alendronic acid monohydrate in aqueous ethanol for 3 hours at reflux with vigorously stirring. Then the mixture was stirred at reflux for additional 15 hours.
• the mixture was cooled to room temperature with stirring.
• Example 11 Preparation of alendronate sodium Form (G) from Alendronate Sodium Trihydrate
• aqueous ethanol (10 ml of ethanol + 1.9 ml of water) was boiled at reflux with stirring for 15 hrs. After cooling to ambient temperature the solid was filtered, washed with absolute ethanol and ether, and dried overnight in a vacuum oven (10-15 mm Hg, ambient temperature) to give 0.9 g of alendronate sodium, containing crystal form G.
• aqueous ethanolic sodium hydroxide was added dropwise to the suspension of alendronic acid monohydrate in aqueous ethanol for 15 minutes at reflux with vigorously stirring. The mixture was then refluxed for additional 15 hours. The mixture was then cooled to room temperature with stirring. The solid was filtered, washed with absolute ethanol, then with absolute ethyl ether, and dried overnight in a vacuum oven (10-15 mm Hg, ambient temperature) to give 5.2 g of alendronate sodium, having crystalline Form H.

Landscapes

• Health & Medical Sciences (AREA)
• Chemical & Material Sciences (AREA)
• Organic Chemistry (AREA)
• General Health & Medical Sciences (AREA)
• Life Sciences & Earth Sciences (AREA)
• Physical Education & Sports Medicine (AREA)
• Pharmacology & Pharmacy (AREA)
• Veterinary Medicine (AREA)
• Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
• General Chemical & Material Sciences (AREA)
• Chemical Kinetics & Catalysis (AREA)
• Bioinformatics & Cheminformatics (AREA)
• Animal Behavior & Ethology (AREA)
• Engineering & Computer Science (AREA)
• Public Health (AREA)
• Medicinal Chemistry (AREA)
• Orthopedic Medicine & Surgery (AREA)
• Rheumatology (AREA)
• Biochemistry (AREA)
• Molecular Biology (AREA)
• Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
• Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
• Peptides Or Proteins (AREA)

Priority Applications (21)

Applications Claiming Priority (6)

Publications (2)

Family

ID=27378576

Family Applications (1)

Country Status (30)

Cited By (8)

Families Citing this family (16)

Citations (7)

Family Cites Families (5)

• 1999
  • 1999-08-27 IL IL14142399A patent/IL141423A/xx not_active IP Right Cessation
  • 1999-08-27 DE DE69932620T patent/DE69932620T2/de not_active Expired - Fee Related
  • 1999-08-27 RO ROA200100219A patent/RO122854B1/ro unknown
  • 1999-08-27 BR BR9913472-1A patent/BR9913472A/pt not_active Application Discontinuation
  • 1999-08-27 SK SK248-2001A patent/SK2482001A3/sk unknown
  • 1999-08-27 HU HU0203078A patent/HUP0203078A3/hu unknown
  • 1999-08-27 SI SI9920070A patent/SI20581B/sl not_active IP Right Cessation
  • 1999-08-27 EP EP99944004A patent/EP1107974B1/en not_active Expired - Lifetime
  • 1999-08-27 ES ES99944004T patent/ES2270613T3/es not_active Expired - Lifetime
  • 1999-08-27 HR HR20010129A patent/HRP20010129A2/hr not_active Application Discontinuation
  • 1999-08-27 KR KR1020077004456A patent/KR20070034132A/ko not_active Ceased
  • 1999-08-27 AT AT99944004T patent/ATE334993T1/de not_active IP Right Cessation
  • 1999-08-27 KR KR1020017002535A patent/KR20010079701A/ko not_active Ceased
  • 1999-08-27 NZ NZ510682A patent/NZ510682A/en not_active IP Right Cessation
  • 1999-08-27 EA EA200100184A patent/EA002739B1/ru not_active IP Right Cessation
  • 1999-08-27 DK DK99944004T patent/DK1107974T3/da active
  • 1999-08-27 PL PL99346347A patent/PL346347A1/xx not_active Application Discontinuation
  • 1999-08-27 AU AU56988/99A patent/AU5698899A/en not_active Abandoned
  • 1999-08-27 CZ CZ2001629A patent/CZ2001629A3/cs unknown
  • 1999-08-27 WO PCT/US1999/019838 patent/WO2000012517A1/en not_active Ceased
  • 1999-08-27 US US09/384,145 patent/US6281381B1/en not_active Expired - Fee Related
  • 1999-08-27 PT PT99944004T patent/PT1107974E/pt unknown
  • 1999-08-27 YU YU14701A patent/YU14701A/sh unknown
  • 1999-08-27 CA CA002341459A patent/CA2341459A1/en not_active Abandoned
  • 1999-08-27 JP JP2000567539A patent/JP2002523514A/ja not_active Withdrawn
  • 1999-08-27 EE EEP200100126A patent/EE04552B1/xx not_active IP Right Cessation
• 2001
  • 2001-02-26 LT LT2001016A patent/LT4888B/lt not_active IP Right Cessation
  • 2001-02-26 NO NO20010957A patent/NO20010957L/no not_active Application Discontinuation
  • 2001-02-26 IS IS5864A patent/IS5864A/is unknown
  • 2001-02-26 BG BG105292A patent/BG65329B1/bg unknown
  • 2001-04-05 LV LVP-01-26A patent/LV12720B/en unknown
  • 2001-07-03 US US09/898,756 patent/US6696601B2/en not_active Expired - Fee Related
• 2009
  • 2009-02-18 JP JP2009035788A patent/JP2009143955A/ja active Pending

Patent Citations (7)

Non-Patent Citations (1)

Also Published As

Similar Documents

Legal Events