US5436158A - Rhizopus host vector system - Google Patents

Rhizopus host vector system Download PDF

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Publication number
US5436158A
US5436158A US08/165,881 US16588193A US5436158A US 5436158 A US5436158 A US 5436158A US 16588193 A US16588193 A US 16588193A US 5436158 A US5436158 A US 5436158A
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US
United States
Prior art keywords
rhizopus
plasmid
vector system
host vector
pleu4
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
US08/165,881
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English (en)
Inventor
Masamichi Takagi
Hiroyuki Horiuchi
Koji Yanai
Kenji Sakaguchi
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Nihon Shokuhin Kako Co Ltd
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Nihon Shokuhin Kako Co Ltd
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Priority to US08/165,881 priority Critical patent/US5436158A/en
Application granted granted Critical
Publication of US5436158A publication Critical patent/US5436158A/en
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C12N1/145—Fungal isolates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/645—Fungi ; Processes using fungi
    • C12R2001/845—Rhizopus
US08/165,881 1990-10-04 1993-12-14 Rhizopus host vector system Expired - Lifetime US5436158A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
US08/165,881 US5436158A (en) 1990-10-04 1993-12-14 Rhizopus host vector system

Applications Claiming Priority (7)

Application Number Priority Date Filing Date Title
JP26735790 1990-10-04
JP2-267357 1990-10-04
JP03062693A JP3133774B2 (ja) 1990-10-04 1991-03-04 新規な宿主−ベクター系
JP3-062693 1991-03-04
US77033091A 1991-10-03 1991-10-03
US98575892A 1992-12-04 1992-12-04
US08/165,881 US5436158A (en) 1990-10-04 1993-12-14 Rhizopus host vector system

Related Parent Applications (1)

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US98575892A Continuation 1990-10-04 1992-12-04

Publications (1)

Publication Number Publication Date
US5436158A true US5436158A (en) 1995-07-25

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ID=17443698

Family Applications (1)

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US08/165,881 Expired - Lifetime US5436158A (en) 1990-10-04 1993-12-14 Rhizopus host vector system

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US (1) US5436158A (ja)
JP (1) JP3133774B2 (ja)

Cited By (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20080194005A1 (en) * 1998-10-06 2008-08-14 Mark Aaron Emalfarb Transformation system in the field of filamentous fungal hosts
US20090099079A1 (en) * 2007-09-07 2009-04-16 Emalfarb Mark A Novel Fungal Enzymes
US20110047656A1 (en) * 1996-10-10 2011-02-24 Gusakov Alexander V Construction of highly efficient cellulase compositions for enzymatic hydrolysis of cellulose
US20110237485A1 (en) * 1996-10-10 2011-09-29 Mark Aaron Emalfarb Chrysosporium Cellulase and Methods of Use
EP2505651A2 (en) 2006-12-10 2012-10-03 Dyadic International, Inc. Isolated fungus with reduced protease activity
US9862956B2 (en) 2006-12-10 2018-01-09 Danisco Us Inc. Expression and high-throughput screening of complex expressed DNA libraries in filamentous fungi
US10435724B2 (en) * 2015-10-13 2019-10-08 Kao Corporation Method for producing C4 dicarboxylic acid

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP6650546B1 (ja) 2019-08-01 2020-02-19 日本食品化工株式会社 α−１，６−グルコシル転移反応を触媒する活性を有するタンパク質

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1986003774A1 (en) * 1984-12-14 1986-07-03 Chr. Hansen's Laboratorium A/S A method of transforming fungi with a vector
WO1990000192A1 (en) * 1988-07-01 1990-01-11 Genencor, Inc. Aspartic proteinase deficient filamentous fungi
JPH0253480A (ja) * 1988-08-19 1990-02-22 Nippon Shokuhin Kako Co Ltd リゾープスの形質転換法
WO1991000920A2 (en) * 1989-07-07 1991-01-24 Unilever N.V. Process for preparing a protein by a fungus transformed by multicopy integration of an expression vector

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1986003774A1 (en) * 1984-12-14 1986-07-03 Chr. Hansen's Laboratorium A/S A method of transforming fungi with a vector
WO1990000192A1 (en) * 1988-07-01 1990-01-11 Genencor, Inc. Aspartic proteinase deficient filamentous fungi
JPH0253480A (ja) * 1988-08-19 1990-02-22 Nippon Shokuhin Kako Co Ltd リゾープスの形質転換法
WO1991000920A2 (en) * 1989-07-07 1991-01-24 Unilever N.V. Process for preparing a protein by a fungus transformed by multicopy integration of an expression vector

Non-Patent Citations (12)

* Cited by examiner, † Cited by third party
Title
Revuelta et al. (Oct. 1986), Proc. Nat. Acad. Sci. USA, vol. 83, pp. 7344 7347. *
Revuelta et al. (Oct. 1986), Proc. Nat. Acad. Sci. USA, vol. 83, pp. 7344-7347.
Sambrook et al., Molecular Cloning: A Laboratory Manual, Second ed., Cold Spring Harbor Laboratory Press, (Cold Spring Harbor, N.Y.), 1989, pp. 17.32 17.44. *
Sambrook et al., Molecular Cloning: A Laboratory Manual, Second ed., Cold Spring Harbor Laboratory Press, (Cold Spring Harbor, N.Y.), 1989, pp. 17.32-17.44.
Saurez et al. (1988), Mol. Gen. Genet. 212:120 123. *
Saurez et al. (1988), Mol. Gen. Genet. 212:120-123.
Yanai et al. (1990), Agric. Biol. Chem. 54(10):2689 2696. *
Yanai et al. (1990), Agric. Biol. Chem. 54(10):2689-2696.
Yanai et al. (1990), Agric. Biol. Chem., vol. 54(10), pp. 2689 2696. (Biol. Abstr. 91(5):AB 361, Ref. No. 49416). *
Yanai et al. (1990), Agric. Biol. Chem., vol. 54(10), pp. 2689-2696. (Biol. Abstr. 91(5):AB-361, Ref. No. 49416).
Yanai et al. (1991), Curr. Genet. 19:221 226. *
Yanai et al. (1991), Curr. Genet. 19:221-226.

Cited By (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8673618B2 (en) 1996-10-10 2014-03-18 Dyadic International (Usa), Inc. Construction of highly efficient cellulase compositions for enzymatic hydrolysis of cellulose
US8916363B2 (en) 1996-10-10 2014-12-23 Dyadic International (Usa), Inc. Construction of Highly efficient cellulase compositions for enzymatic hydrolysis of cellulose
US20110047656A1 (en) * 1996-10-10 2011-02-24 Gusakov Alexander V Construction of highly efficient cellulase compositions for enzymatic hydrolysis of cellulose
US20110045546A1 (en) * 1996-10-10 2011-02-24 Gusakov Alexander V Construction of Highly Efficient Cellulase Compositions for Enzymatic Hydrolysis of Cellulose
US20110237485A1 (en) * 1996-10-10 2011-09-29 Mark Aaron Emalfarb Chrysosporium Cellulase and Methods of Use
US8268585B2 (en) 1998-10-06 2012-09-18 Dyadic International (Usa), Inc. Transformation system in the field of filamentous fungal hosts
US20080194005A1 (en) * 1998-10-06 2008-08-14 Mark Aaron Emalfarb Transformation system in the field of filamentous fungal hosts
EP2505651A2 (en) 2006-12-10 2012-10-03 Dyadic International, Inc. Isolated fungus with reduced protease activity
US8680252B2 (en) 2006-12-10 2014-03-25 Dyadic International (Usa), Inc. Expression and high-throughput screening of complex expressed DNA libraries in filamentous fungi
US9862956B2 (en) 2006-12-10 2018-01-09 Danisco Us Inc. Expression and high-throughput screening of complex expressed DNA libraries in filamentous fungi
US8551751B2 (en) 2007-09-07 2013-10-08 Dyadic International, Inc. BX11 enzymes having xylosidase activity
US20090099079A1 (en) * 2007-09-07 2009-04-16 Emalfarb Mark A Novel Fungal Enzymes
US10435724B2 (en) * 2015-10-13 2019-10-08 Kao Corporation Method for producing C4 dicarboxylic acid

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Publication number Publication date
JPH04218382A (ja) 1992-08-07
JP3133774B2 (ja) 2001-02-13

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