US20160168155A1 - Polymorph of syk inhibitors - Google Patents

Polymorph of syk inhibitors Download PDF

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US20160168155A1
US20160168155A1 US14/907,767 US201414907767A US2016168155A1 US 20160168155 A1 US20160168155 A1 US 20160168155A1 US 201414907767 A US201414907767 A US 201414907767A US 2016168155 A1 US2016168155 A1 US 2016168155A1
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Prior art keywords
bis
mesylate salt
formula
compound
polymorph
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Peter Chee-Chu FUNG
Dimitrios Stefanidis
Dragos Vizitiu
Tim G. ELFORD
Michael Laird HURREY
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Kronos Bio Inc
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Gilead Connecticut Inc
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Assigned to GILEAD CONNECTICUT, INC. reassignment GILEAD CONNECTICUT, INC. ASSIGNMENT OF ASSIGNORS INTEREST (SEE DOCUMENT FOR DETAILS). Assignors: FUNG, PETER CHEE-CHU, HURREY, MICHAEL LAIRD, STEFANIDIS, DIMITRIOS, ELFORD, TIM G., VIZITIU, DRAGOS
Assigned to GILEAD CONNECTICUT, INC. reassignment GILEAD CONNECTICUT, INC. ASSIGNMENT OF ASSIGNORS INTEREST (SEE DOCUMENT FOR DETAILS). Assignors: FUNG, PETER CHEE- CHU, HURREY, MICHAEL LAIRD, STEFANIDIS, DIMITRIOS, ELFORD, TIM G., VIZITIU, DRAGOS
Assigned to GILEAD CONNECTICUT, INC. reassignment GILEAD CONNECTICUT, INC. MERGER AND CHANGE OF NAME (SEE DOCUMENT FOR DETAILS). Assignors: CGI PHARMACEUTICALS, INC., COUGAR MERGER SUB, INC.
Assigned to Kronos Bio, Inc. reassignment Kronos Bio, Inc. ASSIGNMENT OF ASSIGNORS INTEREST (SEE DOCUMENT FOR DETAILS). Assignors: GILEAD CONNECTICUT, INC.
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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D487/00—Heterocyclic compounds containing nitrogen atoms as the only ring hetero atoms in the condensed system, not provided for by groups C07D451/00 - C07D477/00
    • C07D487/02—Heterocyclic compounds containing nitrogen atoms as the only ring hetero atoms in the condensed system, not provided for by groups C07D451/00 - C07D477/00 in which the condensed system contains two hetero rings
    • C07D487/04—Ortho-condensed systems
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P11/00—Drugs for disorders of the respiratory system
    • A61P11/06—Antiasthmatics
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P19/00—Drugs for skeletal disorders
    • A61P19/02—Drugs for skeletal disorders for joint disorders, e.g. arthritis, arthrosis
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P25/00—Drugs for disorders of the nervous system
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P25/00—Drugs for disorders of the nervous system
    • A61P25/02—Drugs for disorders of the nervous system for peripheral neuropathies
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P29/00—Non-central analgesic, antipyretic or antiinflammatory agents, e.g. antirheumatic agents; Non-steroidal antiinflammatory drugs [NSAID]
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P35/00—Antineoplastic agents
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P35/00—Antineoplastic agents
    • A61P35/02—Antineoplastic agents specific for leukemia
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P37/00—Drugs for immunological or allergic disorders
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P37/00—Drugs for immunological or allergic disorders
    • A61P37/02—Immunomodulators
    • A61P37/06—Immunosuppressants, e.g. drugs for graft rejection
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
    • C07C309/00—Sulfonic acids; Halides, esters, or anhydrides thereof
    • C07C309/01—Sulfonic acids
    • C07C309/02—Sulfonic acids having sulfo groups bound to acyclic carbon atoms
    • C07C309/03—Sulfonic acids having sulfo groups bound to acyclic carbon atoms of an acyclic saturated carbon skeleton
    • C07C309/04—Sulfonic acids having sulfo groups bound to acyclic carbon atoms of an acyclic saturated carbon skeleton containing only one sulfo group

Definitions

  • the present disclosure relates to polymorphs and polymorph pharmaceutical compositions of compounds that inhibit Spleen Tyrosine Kinase (Syk) activity.
  • the disclosure also relates to methods of preparing such polymorphs and polymorph pharmaceutical compositions, and the use of such polymorphs and pharmaceutical compositions in treating subjects with various diseases, including cancer and inflammatory conditions.
  • Spleen Tyrosine Kinase Syk activity
  • Syk Spleen Tyrosine Kinase
  • One such compound that has been found to inhibit Syk activity is represented by Formula I:
  • a bis-mesylate salt of the compound of Formula I has been chosen for further development.
  • Such bis-mesylate salt may be depicted in various ways.
  • the bis-mesylate salt may be depicted as the compound of Formula IA, having the molecular structure:
  • a hydrate of the bis-mesylate salt of the compound of Formula I is provided by this disclosure.
  • a monohydrate of the bis-mesylate salt of the compound of Formula I is provided by this disclosure.
  • certain polymorphic forms of the bis-mesylate salt of the compound of Formula I, or a hydrate thereof, are provided by this disclosure.
  • polymorphs of a bis-mesylate salt of the compound of Formula I e.g., a compound of Formula IA
  • polymorphic forms of a hydrate, bis-mesylate salt of the compound of Formula I are provided.
  • polymorphic Forms 3 and 7 of the bis-mesylate salt of the compound of Formula I are provided. Methods of making and using these polymorphic forms are also provided.
  • polymorphic products obtained by the processes described herein e.g., obtained by the described methods of making).
  • compositions comprising one or more polymorphic forms of a bis-mesylate salt of the compound of Formula I (e.g., a compound of Formula IA), or a hydrate thereof, and a pharmaceutically acceptable carrier are provided.
  • Articles of manufacture and unit dosage forms comprising one or more polymorphic forms of a bis-mesylate salt of the compound of Formula I (e.g., a compound of Formula IA), or a hydrate thereof, are provided.
  • Kits comprising one or more polymorphic forms of a bis-mesylate salt of the compound of Formula I (e.g., a compound of Formula IA), or a hydrate thereof, and instructions for use (e.g., instructions for use in SYK-mediated disorder, such as cancer or an autoimmune disease) are also provided.
  • the bis-mesylate salt of the compound of Formula I is a hydrate thereof.
  • the bis-mesylate salt of the compound of Formula I is a monohydrate thereof.
  • the bis-mesylate salt of the compound of Formula I or a hydrate thereof is polymorph Form 3 or polymorph Form 7, or a combination thereof.
  • polymorph of the bis-mesylate salt of the compound of Formula I or a hydrate thereof.
  • a polymorph of a hydrate of the bis-mesylate salt of the compound of Formula I In certain embodiments, provided is a polymorph of a monohydrate of the bis-mesylate salt of the compound of Formula I.
  • Polymorph Forms 3 and Form 7 are provided in this disclosure, as are methods of making and using polymorph Form 3 and Form 7, and pharmaceutical compositions, articles of manufacture, unit dosage forms and kits comprising polymorph Form 3 or Form 7, or both.
  • a polymorph of a monohydrate, bis-mesylate salt of a compound of Formula I is polymorph Form 3, characterized by or having an X-ray diffraction pattern comprising 2 ⁇ -reflections ( ⁇ 0.2 degrees) at 13.8, 16.9, 22.9, and 26.1.
  • a polymorph of a monohydrate, bis-mesylate salt of a compound of Formula I is polymorph Form 3, characterized by or having an X-ray diffraction pattern comprising 2 ⁇ -reflections ( ⁇ 0.2 degrees) at 7.7, 12.9, 17.7, and 18.1.
  • a polymorph of a monohydrate, bis-mesylate salt of a compound of Formula I is polymorph Form 3, characterized by or having an X-ray diffraction pattern comprising 2 ⁇ -reflections ( ⁇ 0.2 degrees) at 7.7, 12.9, 13.8, 16.9, 17.7, 18.1, 22.9, and 26.1.
  • 2 ⁇ -reflections in the X-ray diffraction pattern e.g., of polymorph Form 3, also referred to herein as “Form 3” or “Form III”
  • ⁇ 0.2 degrees can also be expressed as “plus or minus 0.2 degrees 2 ⁇ ”.
  • a polymorph of a monohydrate, bis-mesylate salt of a compound of Formula I is polymorph Form 3, characterized by or having an X-ray diffraction pattern substantially as shown in FIG. 1A .
  • a polymorph of a monohydrate, bis-mesylate salt of a compound of Formula I is polymorph Form 3, characterized by or having an X-ray diffraction pattern substantially as shown in FIG. 1B .
  • a polymorph of a hydrate, bis-mesylate salt of a compound of Formula I is polymorph Form 7, characterized by or having an X-ray diffraction pattern comprising 2 ⁇ -reflections at 4.9 ( ⁇ 0.2 degrees), 9.8 ( ⁇ 0.2 degrees), and 26.7 ( ⁇ 0.4 degrees).
  • a polymorph of a hydrate, bis-mesylate salt of a compound of Formula I is polymorph Form 7, characterized by or having an X-ray diffraction pattern comprising 2 ⁇ -reflections at 4.9 ( ⁇ 0.2 degrees), 9.8 ( ⁇ 0.2 degrees), and 26.7 ( ⁇ 0.3 degrees).
  • a polymorph of a hydrate, bis-mesylate salt of a compound of Formula I is polymorph Form 7, characterized by or having an X-ray diffraction pattern comprising 2 ⁇ -reflections ( ⁇ 0.2 degrees) at 4.9, 9.8, and 26.7.
  • a polymorph of a hydrate, bis-mesylate salt of a compound of Formula I is polymorph Form 7, characterized by or having an X-ray diffraction pattern comprising 2 ⁇ -reflections ( ⁇ 0.2 degrees) at 15.0 and 18.0.
  • a polymorph of a hydrate, bis-mesylate salt of a compound of Formula I is polymorph Form 7, characterized by or having an X-ray diffraction pattern comprising 2 ⁇ -reflections ( ⁇ 0.2 degrees) at 4.9, 9.8, 15.0 and 18.0; and 2 ⁇ -reflections ( ⁇ 0.4 degrees) at 26.7.
  • a polymorph of a hydrate, bis-mesylate salt of a compound of Formula I is polymorph Form 7, characterized by or having an X-ray diffraction pattern comprising 2 ⁇ -reflections ( ⁇ 0.2 degrees) at 4.9, 9.8, 15.0 and 18.0; and 2 ⁇ -reflections ( ⁇ 0.3 degrees) at 26.7.
  • a polymorph of a hydrate, bis-mesylate salt of a compound of Formula I is polymorph Form 7, characterized by or having an X-ray diffraction pattern comprising 2 ⁇ -reflections ( ⁇ 0.2 degrees) at 4.9, 9.8, 15.0, 18.0, and 26.7.
  • a polymorph of a hydrate, bis-mesylate salt of a compound of Formula I is polymorph Form 7, characterized by or having an X-ray diffraction pattern substantially as shown in FIG. 2A .
  • a polymorph of a hydrate, bis-mesylate salt of a compound of Formula I is polymorph Form 7, characterized by or having an X-ray diffraction pattern substantially as shown in FIG.
  • a hydrate of a bis-mesylate salt of the compound of Formula I is a polymorph selected from the group consisting of Form 3 and Form 7; wherein Form 3 is characterized by or has a differential scanning calorimetry profile substantially as shown in FIG. 3A, 3B or 3C , and/or a thermogravametrical analysis profile substantially as shown in FIG. 3B or 3C ; and Form 7 is characterized by or has a differential scanning calorimetry profile substantially as shown in FIG. 4A, 4B or 4C , and/or a thermogravametrical analysis profile substantially as shown in FIG. 4B or 4C .
  • polymorph Form 3 is characterized by or has an X-ray diffraction pattern substantially as shown in FIG. 1A or 1B .
  • polymorph Form 7 is characterized by or has an X-ray diffraction pattern substantially as shown in FIG. 2A or 2B .
  • the compound of Formula (IA), or hydrate thereof is crystalline.
  • X-ray diffraction pattern comprising 2 ⁇ -reflections ( ⁇ 0.2 degrees) selected from the group consisting of Form 3: 13.8, 16.9, 22.9, and 26.1; and Form 7: 4.9, 9.8, and 26.7.
  • an X-ray diffraction pattern of a polymorph of a monohydrate of a compound of Formula IA comprises 2 ⁇ -reflections ( ⁇ 0.2 degrees) characteristic of Form 3. In some embodiments, an X-ray diffraction pattern of a polymorph of a monohydrate of a compound of Formula IA comprises 2 ⁇ -reflections ( ⁇ 0.2 degrees) characteristic of Form 7. In some embodiments, an X-ray diffraction pattern of polymorph Form 3 is represented in FIG. 1A or 1 B. In some embodiments, an X-ray diffraction pattern of polymorph Form 7 is represented in FIG. 2A or 2B .
  • a polymorph of the compound of Formula (IA) is a polymorph selected from the group consisting of Form 3 and Form 7; wherein Form 3 has a differential scanning calorimetry profile represented in FIG. 3A, 3B or 3C , and Form 7 has a differential scanning calorimetry profile represented in FIG. 4A, 4B or 4C .
  • a polymorph of the compound of Formula (IA) is polymorph Form 3 having a differential scanning calorimetry profile represented in FIG. 3A, 3B or 3C and/or an X-ray diffraction pattern represented in FIG. 1A or 1B .
  • a polymorph of the compound of Formula (IA) is polymorph Form 7 having a differential scanning profile represented in FIG. 4A, 4B or 4C and/or an X-ray diffraction pattern represented in FIG. 2A or 2B .
  • polymorph is bioequivalent to the polymorph of any of the foregoing embodiments.
  • polymorph Form 3 which is a polymorph of a monohydrate, bis-mesylate salt of the compound of Formula I
  • polymorph Form 7 which is a polymorph of a hydrate, bis-mesylate salt of the compound of Formula I
  • a method of preparing a production scale amount of polymorph Form 3 (which is a polymorph of a monohydrate, bis-mesylate salt of the compound of Formula I), comprising adding an amount of polymorph Form 3 seeds and a solvent to polymorph Form 7 (which is a polymorph of a hydrate, bis-mesylate salt of the compound of Formula I) to form a mixture; wherein the polymorph Form 3 has an X-ray diffraction pattern comprising 2 ⁇ -reflections ( ⁇ 0.2 degrees) selected from the group consisting of A: 13.8, 16.9, 22.9, and 26.1; B: 7.7, 12.9, 17.7, and 18.1; and C: 7.7, 12.9, 13.8, 16.9, 17.7, 18.1, 22.9, and 26.1; and wherein the polymorph Form 7 has an X-ray diffraction pattern comprising 2 ⁇ -reflections ( ⁇ 0.2 degrees) selected from the group consisting of A: 4.9, 9.8, and 26.7; B: 15.0 and 18.0; and C
  • the polymorph Form 3 has an X-ray diffraction pattern comprising 2 ⁇ -reflections ( ⁇ 0.2 degrees) selected from the group consisting of A: 13.8, 16.9, 22.9, and 26.1; B: 7.7, 12.9, 17.7, and 18.1; and C: 7.7, 12.9, 13.8, 16.9, 17.7, 18.1, 22.9, and 26.1; and the polymorph Form 7 has an X-ray diffraction pattern comprising 2 ⁇ -reflections ( ⁇ 0.2 degrees) at 4.9, 9.8, and 26.7.
  • the polymorph Form 3 has an X-ray diffraction pattern comprising 2 ⁇ -reflections ( ⁇ 0.2 degrees) selected from the group consisting of A: 13.8, 16.9, 22.9, and 26.1; B: 7.7, 12.9, 17.7, and 18.1; and C: 7.7, 12.9, 13.8, 16.9, 17.7, 18.1, 22.9, and 26.1; and the polymorph Form 7 has an X-ray diffraction pattern comprising 2 ⁇ -reflections ( ⁇ 0.2 degrees) at 4.9 and 9.8, and 2 ⁇ -reflections ( ⁇ 0.4 degrees) at 26.7.
  • the polymorph Form 3 has an X-ray diffraction pattern comprising 2 ⁇ -reflections ( ⁇ 0.2 degrees) selected from the group consisting of A: 13.8, 16.9, 22.9, and 26.1; B: 7.7, 12.9, 17.7, and 18.1; and C: 7.7, 12.9, 13.8, 16.9, 17.7, 18.1, 22.9, and 26.1; and the polymorph Form 7 has an X-ray diffraction pattern comprising 2 ⁇ -reflections ( ⁇ 0.2 degrees) at 4.9 and 9.8, and 2 ⁇ -reflections ( ⁇ 0.3 degrees) at 26.7.
  • the method further comprises isolating polymorph Form 3.
  • the solvent comprises acetone.
  • the solvent comprises acetone and water.
  • the ratio of water to acetone is about 1:15 to about 1:40; or about 1:18 to about 1:22.
  • the method further comprises agitating the mixture; heating the mixture; and cooling the mixture to provide polymorph Form 3. It is understood that the methods detailed herein may also be carried out on a non-production scale, such as a method of preparing a less than production scale amount of polymorph Form 3.
  • polymorph Form 3 which is a polymorph of a monohydrate of a compound of Formula IA:
  • the method further comprises adding an amount of polymorph Form 3 seeds and at least one solvent to polymorph Form 7, which is a polymorph of a hydrate of a compound of Formula IA, to form a mixture; and isolating polymorph Form 3 produced.
  • the at least one solvent is acetone.
  • the at least one solvent further comprises water.
  • the at least one solvent is acetone and further comprises water.
  • the ratio of water to acetone is about 1:15 to about 1:40. In other embodiments, the ratio of water to acetone is about 1:18 to about 1:22.
  • the method further comprises agitating the mixture; heating the mixture; and cooling the mixture to provide polymorph Form 3.
  • polymorph Form 3 which is polymorph of a monohydrate, bis-mesylate salt of a compound of Formula I, comprising: adding a solvent to a compound of Formula I to form a mixture; adding an amount of methanesulfonic acid to the mixture; heating the mixture; adding an amount of polymorph Form 3 seeds to the mixture; and cooling the mixture; wherein the method is capable of producing polymorph Form 3.
  • a method of preparing a production scale amount of polymorph Form 3, which is a polymorph of a monohydrate, bis-mesylate salt of a compound of Formula I comprising: adding a solvent to a compound of Formula I to form a mixture; adding an amount of methanesulfonic acid to the mixture; heating the mixture; adding an amount of polymorph Form 3 seeds to the mixture; and cooling the mixture; wherein an X-ray diffraction pattern of the polymorph Form 3 comprises 2 ⁇ -reflections ( ⁇ 0.2 degrees) selected from the group consisting of A: 13.8, 16.9, 22.9, and 26.1; B: 7.7, 12.9, 17.7, and 18.1; and C: 7.7, 12.9, 13.8, 16.9, 17.7, 18.1, 22.9, and 26.1.
  • the mixture formed from adding solvent to the compound of Formula I may be a heterogeneous mixture.
  • the method further comprises isolating the polymorph Form 3.
  • the amount of methanesulfonic acid is between 1.8 and 3.2 molar equivalents, between 1.8 and 3.0 molar equivalents, between 1.95 and 3.0 molar equivalents, or between 2.0 and 2.4 molar equivalents with respect to one molar equivalent of the compound of Formula I. It is understood that the methods detailed herein may also be carried out on a non-production scale, such as a method of preparing a less than production scale amount of polymorph Form 3.
  • polymorph Form 3 which is a polymorph of a monohydrate, bis-mesylate salt of a compound of Formula I:
  • the amount of methanesulfonic acid is between 2.0 and 2.4 molar equivalents with respect to one molar equivalent of the compound of Formula I.
  • polymorph Form 3 which is a polymorph of a monohydrate, bis-mesylate salt of a compound of Formula I, prepared by a method of any of the foregoing embodiments.
  • a production scale amount of polymorph Form 3 which is a polymorph of a monohydrate, bis-mesylate salt of a compound of Formula I, prepared by a production scale method of the foregoing embodiments.
  • polymorph Form 7 which is a polymorph of a hydrate, bis-mesylate salt of a compound of Formula I:
  • polymorph Form 7 is a polymorph of a hydrate, bis-mesylate salt of a compound of Formula I, prepared by a method of any of the foregoing embodiments.
  • polymorph Form 3 which is a polymorph of a monohydrate of a compound of Formula IA:
  • a pharmaceutical composition comprising a bis-mesylate salt of a compound of Formula I, a hydrate thereof, or a polymorph according to any of the foregoing embodiments.
  • an article of manufacture comprising a bis-mesylate salt of a compound of Formula I, or a hydrate thereof; or a polymorph or a pharmaceutical composition according to any of the foregoing embodiments.
  • a method of treating a condition in a subject in need thereof comprising administering to the subject a bis-mesylate salt of a compound of Formula I, or a hydrate thereof; or a polymorph of the bis-mesylate salt of a compound of Formula I, or a hydrate thereof; or a pharmaceutical composition comprising any of the foregoing embodiments, wherein the condition is selected from the group consisting of cancer and autoimmune disease.
  • the condition is selected from the group consisting of acute lymphocytic leukemia (ALL), acute myeloid leukemia (AML), chronic lymphocytic leukemia (CLL), small lymphocytic lymphoma (SLL), myelodysplastic syndrome (MDS), myeloproliferative disease (MPD), chronic myeloid leukemia (CML), multiple myeloma (MM), non-Hodgkin's lymphoma (NHL), mantle cell lymphoma (MCL), follicular lymphoma (FL), Waldestrom's macroglobulinemia (WM), T-cell lymphoma, B-cell lymphoma, diffuse large B-cell lymphoma (DLBCL), lymphoplasmacytic lymphoma (LPL), and marginal zone lymphoma (MZL).
  • ALL acute lymphocytic leukemia
  • AML acute myeloid leukemia
  • CLL chronic lymphocytic leukemia
  • SLL small lympho
  • the condition is non-Hodgkin's lymphoma.
  • the NHL is indolent non-Hodgkin's lymphoma (iNHL).
  • the iNHL is refractory iNHL.
  • the iNHL is non-FL iNHL.
  • the condition is selected from the group consisting of asthma, rheumatoid arthritis, multiple sclerosis, and lupus.
  • the subject is human.
  • FIGS. 1A and 1B are exemplary X-ray powder diffraction pattern (XRPD) patterns of polymorph Form 3.
  • FIGS. 2A and 2B are exemplary XRPD patterns of polymorph Form 7.
  • the XRPD pattern in FIG. 2B was obtained at 25° C. and 53% relative humidity (RH).
  • FIG. 3A is an exemplary differential scanning calorimetry (DSC) plot of polymorph Form 3.
  • FIGS. 3B and 3C are exemplary DSC and thermal gravimetric analysis (TGA) plots of polymorph Form 3.
  • FIG. 4A is an exemplary DSC plot of polymorph Form 7.
  • FIGS. 4B and 4C are exemplary DSC and TGA plots of polymorph Form 7.
  • FIG. 5 shows the results of dynamic vapor sorption (DVS) plot of ( FIG. 5A ) polymorph Form 3, and ( FIG. 5B ) polymorph Form 7.
  • FIGS. 5C and 5D show the results of a DVS plot of polymorph Form 3 and polymorph Form 7, respectively.
  • FIG. 6 is an XRPD pattern of polymorph Form 3 before and after DVS measurements.
  • FIG. 7 shows a comparison of dissolution rates between polymorph Form 3 and the mono-mesylate (mono-MSA) salt of a compound of Formula I at pH 6.8.
  • FIG. 8 shows a plot summarizing the results of a PK study of mono- and bis-MSA salt form in dogs that have been pretreated with either pentagastrin or famotidine (an acid suppressant).
  • FIG. 9 shows a simulated XRPD pattern of polymorph Form 3.
  • FIG. 10A shows a view of polymorph Form 3 from the crystal structure showing the numbering scheme employed.
  • FIG. 10B illustrates the hydrogen bonding interactions of polymorph Form 3.
  • FIG. 11 illustrates the infinite chains of polymorph Form 3, mesylate and water molecules viewed down the crystallographic a axis.
  • FIG. 12 illustrates the packing of polymorph Form 3 down the crystallographic b axis.
  • FIG. 13 shows an exemplary proton NMR spectrum of polymorph Form 3.
  • FIG. 14 shows an exemplary proton NMR spectrum of polymorph Form 7.
  • the term “about” includes and describes the value or parameter per se.
  • “about x” includes and describes “x” per se.
  • the term “about” when used in association with a measurement, or used to modify a value, a unit, a constant, or a range of values refers to variations of ⁇ 10%.
  • “about 2:8” in some embodiments includes 1.8-2.2:7.2-8.8.
  • adding does not limit the order, method or how the materials being added are combined, unless indicated otherwise.
  • “adding A to B” may also describe “adding B to A”.
  • “adding A and B to C” may also describe the various other combinations such as “adding A to B and C”, “adding A and C to B”, “adding B to A and C”, “adding B and C to A”, and “adding C to A and B”.
  • the pharmaceutically acceptable salt of the compound of Formula I is a mesylate salt, or a hydrate thereof. In one variation, the pharmaceutically acceptable salt of the compound of Formula I is a bis-mesylate salt, or a hydrate thereof.
  • a bis-mesylate salt, or a hydrate thereof, of a compound of Formula I is provided. It should be understood that “bis-mesylate salt” may also be referred to herein as “bis-MSA salt”.
  • a hydrate, bis-mesylate salt of a compound of Formula I is provided.
  • a monohydrate, bis-mesylate salt of a compound of Formula I is provided.
  • a polymorph of any of the foregoing is provided.
  • Form 3 which is a polymorph of a monohydrate, bis-mesylate salt of a compound of Formula I
  • Form 7 which is a polymorph of a hydrate, bis-mesylate salt of a compound of Formula I, is provided.
  • the bis-mesylate salt of a compound of Formula I may be depicted herein in various ways.
  • a bis-mesylate salt may be represented by Formula IA:
  • a bis-mesylate salt of the compound of Formula I is depicted as Formula IA above, the ionic form (e.g., the cationic form of the compound of formula I and the anionic form of the methanesulfonic acid) is intended.
  • a bis-mesylate salt may be represented by Formula IB:
  • the bis-mesylate salt may be a hydrate thereof.
  • the bis-mesylate salt, as depicted by Formula IA or IB may be a monohydrate, bis-mesylate salt.
  • a monohydrate, bis-mesylate salt of a compound of Formula I may also be represented by Formula IC:
  • the monohydrate, bis-mesylate salt of a compound of Formula I has increased solubility at higher pH compared to the mono-mesylate salt of a compound of Formula I. It should be understood that “mono-mesylate salt” may also be referred to herein as “mono-MSA salt”.
  • the bis-mesylate salt of a compound of Formula I in comparison to the mono-mesylate salt of a compound of Formula I provides increased bioavailability and the ability to offset the effect of acid reducing agents on the pharmacokinetic (PK) profile of the compound of Formula I.
  • a hydrate of a bis-mesylate salt of a compound of Formula I may be represented by Formula ID:
  • y is at least 0.5.
  • y is at least 1, at least 1.5, at least 2, at least 2.5, at least 3, or at least 4, or between 0.5 and 5, between 0.5 and 4, between 0.5 and 2, between 0.5 and 1.5, or about 0.5, about 1, about 1.5, about 2, about 3, or about 4.
  • y is an integer.
  • the compound of Formula ID is a monohydrate, bis-mesylate salt.
  • the compound of Formula ID is a bis-hydrate, bis-mesylate salt.
  • variable “y” in Formula ID represents the variability of the water content in the hydrate of the bis-mesylate salt.
  • the hydrate, bis-mesylate salt may be represented by Formula IE:
  • y is at least 0.5.
  • y is at least 1, at least 1.5, at least 2, at least 2.5, at least 3, or at least 4, or between 0.5 and 5, between 0.5 and 4, between 0.5 and 2, between 0.5 and 1.5, or about 0.5, about 1, about 1.5, about 2, about 3, or about 4.
  • y is an integer.
  • the compound of Formula IE is a bis-hydrate, bis-mesylate salt.
  • y is a non-integer.
  • a hydrate of a bis-mesylate salt of a compound of Formula I may have varying amounts of water.
  • polymorphs of a mesylate salt of a compound of Formula I, or a hydrate thereof are provided.
  • polymorphs of a monohydrate, bis-mesylate salt of a compound of Formula I are provided.
  • a polymorph of a compound of Formula IA or IB, or a hydrate thereof is provided.
  • a polymorph of a compound of Formula IC, ID or IE is provided.
  • the polymorphs described herein may be characterized by a variety of solid state analytical data, including for example, by X-ray powder diffraction pattern (XRPD), differential scanning calorimetry (DSC) and thermal gravimetric analysis (TGA).
  • XRPD X-ray powder diffraction pattern
  • DSC differential scanning calorimetry
  • TGA thermal gravimetric analysis
  • a bis-mesylate salt of a compound of Formula I, or a hydrate thereof involves the development of a bioavailable and stable compound.
  • variations in the crystal structure of a pharmaceutical drug substance may affect the dissolution rate (which may affect bioavailability, etc.), manufacturability (e.g., ease of handling, ability to consistently prepare doses of known strength) and stability (e.g., thermal stability, shelf life, etc.) of a pharmaceutical drug product, particularly when formulated in a solid oral dosage form.
  • polymorph Form 3 which is a polymorph of a monohydrate, bis-mesylate salt of a compound of Formula I.
  • Form 3 is crystalline.
  • polymorph Form 3 which is a polymorph of a monohydrate of a compound of Formula IA:
  • polymorph Form 3 is a crystalline form of a monohydrate, bis-mesylate salt of Formula IA.
  • the bis-mesylate salt is depicted as Formula IA above, the ionic form (e.g., the cationic form of the compound of Formula I and the anionic form of the methanesulfonic acid) is intended.
  • polymorph Form 3 which is a polymorph of a monohydrate of a compound of Formula IB:
  • polymorph Form 3 is a crystalline form of a monohydrate, bis-mesylate salt of Formula IB.
  • polymorph Form 3 which is a polymorph of a compound of Formula IC:
  • polymorph Form 3 is a crystalline form of a monohydrate, bis-mesylate salt of a compound of Formula I.
  • polymorph Form 3 which is a polymorph of a compound of Formula ID:
  • polymorph Form 3 is a crystalline form of a monohydrate, bis-mesylate salt of Formula ID.
  • polymorph Form 3 which is a polymorph of a compound of Formula IE:
  • polymorph Form 3 is a crystalline form of a monohydrate, bis-mesylate salt of Formula IE.
  • polymorph Form 3 is a crystalline form of a monohydrate, bis-mesylate salt of Formula IE.
  • polymorph Form 3 is a polymorph of a monohydrate, bis-mesylate salt of Formula I.
  • bis-mesylate salt may be depicted in various ways, including as a compound of Formula IA or IB.
  • the monohydrate, bis-mesylate salt may be depicted by Formula IC, ID (wherein y is 1) or IE (wherein y is 1).
  • polymorph Form 3 is characterized by or has an X-ray powder diffraction (XRPD) pattern substantially as shown in FIG. 1A or 1B .
  • XRPD X-ray powder diffraction
  • polymorph Form 3 is characterized by or has an X-ray diffraction (XRPD) pattern comprising 2 ⁇ -reflections ( ⁇ 0.2 degrees): 13.8, 16.9, 22.9, and 26.1.
  • polymorph Form 3 is characterized by or has an X-ray diffraction (XRPD) pattern comprising at least one or more; at least two or more; or at least three or more of the 2 ⁇ -reflections ( ⁇ 0.2 degrees): 13.8, 16.9, 22.9, and 26.1.
  • polymorph Form 3 is characterized by or has an X-ray diffraction comprising 2 ⁇ -reflections ( ⁇ 0.2 degrees): 7.7, 12.9, 17.7, and 18.1.
  • polymorph Form 3 is characterized by or has an X-ray diffraction pattern comprising 2 ⁇ -reflections ( ⁇ 0.2 degrees) at 7.7, 12.9, 13.8, 16.9, 17.7, 18.1, 22.9, and 26.1.
  • polymorph Form 3 is characterized by or has an X-ray diffraction (XRPD) pattern comprising at least three or more; at least four or more; or at least five or more of the 2 ⁇ -reflections ( ⁇ 0.2 degrees): 7.7, 12.9, 13.8, 16.9, 17.7, 18.1, 22.9, and 26.1.
  • XRPD X-ray diffraction
  • polymorph Form 3 is characterized by or has a DSC plot substantially as shown in FIG. 3A, 3B or 3C .
  • the term “substantially as shown in” when referring to an X-ray powder diffraction pattern or a differential scanning calorimetry profile means that a pattern or profile that is not necessarily identical to those depicted herein, but that falls within the limits of experimental error or deviations, when considered by one of ordinary skill in the art, would be encompassed.
  • polymorph Form 3 may also have one or more, two or more, three or more, four or more, five or more, or all of the following properties (i)-(vi):
  • polymorph Form 7 which is a polymorph of a hydrate, bis-mesylate salt a compound of Formula I.
  • polymorph Form 7 which is a polymorph of a compound of Formula IB:
  • polymorph Form 7 is a variable hydrate of a bis-mesylate salt of a compound of Formula I.
  • a variable hydrate may have varying water content.
  • polymorph Form 7 is a polymorph of a bis-mesylate salt having between 1.8% to 10% water.
  • Various factors may affect the water content of polymorph Form 7, including, for example, the relative humidity conditions at which polymorph Form 7 is characterized.
  • polymorph Form 7 is a polymorph of a hydrate, bis-mesylate salt that comprises at least two molecules of water.
  • polymorph Form 7 may be depicted by Formula ID:
  • y is at least 0.5.
  • y is at least 1, at least 1.5, at least 2, at least 2.5, at least 3, or at least 4, or between 0.5 and 5, between 0.5 and 4, between 0.5 and 2, between 0.5 and 1.5, or about 0.5, about 1, about 1.5, about 2, about 3, or about 4.
  • y is an integer.
  • the compound of Formula ID is a bis-hydrate, bis-mesylate salt.
  • y is a non-integer.
  • polymorph Form 7 is a polymorph of a hydrate, bis-mesylate salt that comprises at least two molecules of water.
  • polymorph Form 7 may be depicted by Formula IE:
  • y is at least 0.5.
  • y is at least 1, at least 1.5, at least 2, at least 2.5, at least 3, or at least 4, or between 0.5 and 5, between 0.5 and 4, between 0.5 and 2, between 0.5 and 1.5, or about 0.5, about 1, about 1.5, about 2, about 3, or about 4.
  • y is an integer.
  • the compound of Formula IE is a bis-hydrate, bis-mesylate salt.
  • y is a non-integer.
  • polymorph Form 7 refers to the polymorph Form 7, which is a polymorph of a hydrate, bis-mesylate salt of a compound of Formula I.
  • a bis-mesylate salt may be depicted in various ways, including as a compound of Formula IA or IB.
  • a bis-mesylate salt having varying water content may be depicted by Formula ID.
  • polymorph Form 7 is characterized by or has an X-ray diffraction (XRPD) pattern comprising 2 ⁇ -reflections ( ⁇ 0.2 degrees) at 4.9 and 9.8, and 2 ⁇ -reflections ( ⁇ 0.4 degrees) at 26.7.
  • polymorph Form 7 is characterized by or has an X-ray diffraction (XRPD) pattern comprising 2 ⁇ -reflections ( ⁇ 0.2 degrees) at 4.9 and 9.8, and 2 ⁇ -reflections ( ⁇ 0.3 degrees) at 26.7.
  • polymorph Form 7 is characterized by or has an X-ray diffraction (XRPD) pattern comprising 2 ⁇ -reflections ( ⁇ 0.2 degrees): 4.9, 9.8, and 26.7.
  • polymorph Form 7 is characterized by or has an X-ray diffraction (XRPD) pattern comprising at least one or more; or at least two or more of 2 ⁇ -reflections at 4.9 ( ⁇ 0.2 degrees), 9.8 ( ⁇ 0.2 degrees), and 26.7 ( ⁇ 0.4 degrees). In some variations, polymorph Form 7 is characterized by or has an X-ray diffraction (XRPD) pattern comprising at least one or more; or at least two or more of 2 ⁇ -reflections at 4.9 ( ⁇ 0.2 degrees), 9.8 ( ⁇ 0.2 degrees), and 26.7 ( ⁇ 0.3 degrees).
  • XRPD X-ray diffraction
  • polymorph Form 7 is characterized by or has an X-ray diffraction (XRPD) pattern comprising at least one or more; or at least two or more of 2 ⁇ -reflections ( ⁇ 0.2 degrees): 4.9, 9.8, and 26.7.
  • XRPD X-ray diffraction
  • polymorph Form 7 is characterized by or has an X-ray diffraction pattern comprising 2 ⁇ -reflections ( ⁇ 0.2 degrees): 15.0 and 18.0. In some embodiments, polymorph Form 7 is characterized by or has an X-ray diffraction pattern comprising 2 ⁇ -reflections ( ⁇ 0.2 degrees) at 4.9, 9.8, 15.0 and 18.0, and 2 ⁇ -reflections ( ⁇ 0.4 degrees) at 26.7. In some embodiments, polymorph Form 7 is characterized by or has an X-ray diffraction pattern comprising 2 ⁇ -reflections ( ⁇ 0.2 degrees) at 4.9, 9.8, 15.0 and 18.0, and 2 ⁇ -reflections ( ⁇ 0.3 degrees) at 26.7. In some embodiments, polymorph Form 7 is characterized by or has an X-ray diffraction pattern comprising 2 ⁇ -reflections ( ⁇ 0.2 degrees) at 4.9, 9.8, 15.0, 18.0, and 26.7.
  • polymorph Form 7 is characterized by or has an X-ray diffraction (XRPD) pattern comprising at least three or more; at least four or more; or each of the 2 ⁇ -reflections at 4.9 ( ⁇ 0.2 degrees), 9.8 ( ⁇ 0.2 degrees), 15.0 ( ⁇ 0.2 degrees), 18.0 ( ⁇ 0.2 degrees), and 26.7 ( ⁇ 0.4 degrees).
  • polymorph Form 7 is characterized by or has an X-ray diffraction (XRPD) pattern comprising at least three or more; at least four or more; or each of the 2 ⁇ -reflections at 4.9 ( ⁇ 0.2 degrees), 9.8 ( ⁇ 0.2 degrees), 15.0 ( ⁇ 0.2 degrees), 18.0 ( ⁇ 0.2 degrees), and 26.7 ( ⁇ 0.3 degrees).
  • polymorph Form 7 is characterized by or has an X-ray diffraction (XRPD) pattern comprising at least three or more; at least four or more; or each of the 2 ⁇ -reflections ( ⁇ 0.2 degrees) at 4.9, 9.8, 15.0, 18.0, and 26.7.
  • XRPD X-ray diffraction
  • +0.2 degrees, +0.3 degrees, and +0.4 degrees can also be expressed as “plus or minus 0.2 degrees 2 ⁇ ”, “plus or minus 0.3 degrees 2 ⁇ ”, and “plus or minus 0.4 degrees 2 ⁇ ”, respectively.
  • polymorph Form 7 is also characterized by or has an XRPD pattern substantially as shown in FIG. 2A or 2B . It should be understood, however, that relative intensities and assignments of the peaks of polymorphic forms depicted in the figures can vary depending on a number of factors, including sample preparation, mounting, and the instrument and analytical procedure and settings used to obtain the spectrum. As such, the peak observed in the figures and assignments listed herein (including in FIGS. 2A and 2B for polymorph Form 7) are intended to encompass variations of plus or minus 0.2 degrees 2 ⁇ . Further, in certain instances, the XRPD pattern of polymorph Form 7 may be moisture-dependent or vary based on the relative humidity at which Form 7 is characterized. For example, the XRPD in FIG. 2B was obtained at 25° C. and 53% relative humidity (RH).
  • RH relative humidity
  • polymorph Form 7 is provided as a compound intermediate in the preparation of polymorph Form 3. In some embodiments, polymorph Form 7 is provided in a reaction mixture comprising polymorph Form 3. In some methods of making Form 3 from a compound of Formula I (which may also be referred to as the free base of Formula I), Form 7 is an intermediate in the reaction pathway.
  • crystalline refers to a solid phase in which the material has a regular ordered internal structure at the molecular level and gives a distinctive X-ray diffraction pattern with defined peaks. Such materials when heated sufficiently will also exhibit the properties of a liquid, but the change from solid to liquid is characterized by a phase change, typically first order (melting point).
  • polymorph Form 3 is substantially crystalline.
  • a compound that is substantially crystalline e.g., polymorph Form 3 has greater than 50%; or greater than 55%; or greater than 60%; or greater than 65%; or greater than 70%; or greater than 75%; or greater than 80%; or greater than 85%; or greater than 90%; or greater than 95%, or greater than 99% of the compound present in a composition in crystalline form.
  • a compound that is substantially crystalline (e.g., polymorph Form 3) has no more than about 20%, or no more than about 10%, or no more than about 5%, or no more than about 2% in the amorphous form.
  • a compound that is substantially crystalline e.g., polymorph Form 3 has no more than about 20%, or no more than about 10%, or no more than about 5%, or no more than about 2% in the non-crystalline form.
  • bioequivalence between two polymorphs refers to polymorphs having substantially similar bioavailability, substantially similar efficacy, substantially similar safety profiles, or a combination thereof.
  • bioequivalence refers to polymorphs that exhibit substantially similar pharmacokinetic (PK) profiles or therapeutic effects. Bioequivalence may be demonstrated through several in vivo and in vitro methods. These methods may include, for example, pharmacokinetic, pharmacodynamic, clinical and in vitro studies.
  • bioequivalence can be demonstrated using any suitable pharmacokinetic measures or combination of pharmacokinetic measures known in the art, including loading dose, steady-state dose, initial or steady-state concentration of drug, biological half-life, elimination rate, area under the curve (AUC), clearance, the peak blood or plasma concentration (C max ), time to peak concentration (T max ), bioavailability and potency.
  • bioequivalence is achieved with similar dosing amounts. In alternative embodiments, bioequivalence is achieved with different dosing amounts.
  • polymorph Form 3 described herein is prepared by converting Form 7 into Form 3. Converting Form 7 into Form 3 may be facilitated by adding an amount of polymorph Form 3 “seeds” to Form 7. In some embodiments, the methods of preparing Form 3 require forming the polymorph Form 7 as a compound intermediate.
  • polymorph Form 3 which is a polymorph of a monohydrate, bis-mesylate salt of a compound of Formula I, comprising:
  • polymorph Form 3 seeds and at least one solvent to polymorph Form 7 (which is a polymorph of a hydrate, bis-mesylate salt of a compound of Formula I) to form a mixture; and
  • the method further comprises isolating the polymorph Form 3 from the mixture.
  • a method of preparing a production scale amount of polymorph Form 3, which is a polymorph of a monohydrate, bis-mesylate salt of a compound of Formula I, comprising adding an amount of polymorph Form 3 seeds and at least one solvent to polymorph Form 7 (which is a polymorph of a hydrate, bis-mesylate salt of a compound of Formula I) to form a mixture; and isolating polymorph Form 3.
  • the amount of seed is an amount sufficient to initiate nucleation. In some embodiments, the amount of seed is an amount that reduces the time it takes to convert Form 7 to Form 3 compared to conversion without the seed material. In some embodiments, adding an amount of Form 3 seeds reduces Form 7 to Form 3 conversion time by about 10% to about 20%; about 20% to about 50%; about 30% to about 60%; about 40% to about 75%; or about 50% to about 80%, compared to conversion time without the addition of Form 3 seed.
  • adding an amount of Form 3 seeds reduces Form 7 to Form 3 conversion time by at least about 10%; at least about 25%; at least about 40%; at least about 50%; at least about 60%; at least about 75%; or at least about 80%, compared to conversion time without the addition of Form 3 seeds.
  • the methods provide adding an amount of polymorph Form 3 seeds to Form 7.
  • the amount of Form 3 seeds is substantially smaller than the amount of Form 7 to be converted and is also substantially smaller than the amount of Form 3 obtained by the methods described herein.
  • the amount of Form 3 seeds added to polymorph Form 7 is between 0.01 molar % and 5 molar %.
  • the amount of Form 3 seeds added to polymorph Form 7 is between 0.01 molar % and 3 molar % of polymorph Form 7.
  • the amount of Form 3 seeds added to polymorph Form 7 is between 0.01 molar % and 2 molar % of polymorph Form 7.
  • the amount of Form 3 seeds added to polymorph Form 7 is between 0.01 molar % and 1 molar % of polymorph Form 7. In some embodiments, the amount of Form 3 seeds added to polymorph Form 7 is between 0.1 molar % and 2 molar % of polymorph Form 7. In some embodiments, the amount of Form 3 seeds added to polymorph Form 7 is between 0.1 molar % and 1 molar % of polymorph Form 7. In some embodiments, the amount of Form 3 seeds added to polymorph Form 7 is about 1 molar % of polymorph Form 7.
  • the amount of Form 3 seeds added to polymorph Form 7 is between 0.001 and 0.1 weight percent of polymorph Form 7. In some embodiments, the amount of Form 3 seeds added to polymorph Form 7 is between 0.01 and 0.1 weight percent of polymorph Form 7. In some embodiments, the amount of Form 3 seeds added to polymorph Form 7 is between 0.01 and 0.08 weight percent of polymorph Form 7. In some embodiments, the amount of Form 3 seeds added to polymorph Form 7 is about 0.015 weight percent of polymorph Form 7.
  • one or more of the steps of the method to prepare polymorph Form 3 from Form 7 may be omitted or the order of the steps may be varied.
  • the methods of making Form 3 do not require adding seeds of Form 3 to Form 7.
  • the method comprises the steps of heating and cooling the mixture.
  • polymorph Form 7 is not isolated from the reaction mixture but generated in situ and converted to Form 3.
  • Form 3, including seeds thereof, is obtained through alternative methods, for instance, the method described in Example 11. In some embodiments, the method of preparing Form 3 does not comprise adding seeds of Form 3.
  • a production scale amount of polymorph Form 3 is greater than 1 kg, 5 kg, 10 kg, 20 kg, 50 kg, 100 kg, 200 kg, or 500 kg. In some embodiments, a production scale amount of polymorph Form 3 is between 1 kg and 500 kg. In some embodiments, a production scale amount of polymorph Form 3 is between 10 kg and 300 kg. In some embodiments, a production scale amount of polymorph Form 3 is between 50 kg and 500 kg.
  • the mixture is heated to reflux. In some embodiments, the mixture is heated to a temperature between about 40° C. and about 65° C. In some embodiments, the mixture is heated to a temperature between about 45° C. and about 65° C. In some embodiments, the mixture is heated to a temperature of about 55° C.
  • the mixture may be agitated. In some embodiments, the mixture is agitated to facilitate the formation of the Form 3 product. In some embodiments, agitation increases dispersion of the components in a mixture. Well-dispersed components in a mixture prevent high concentrations of certain materials accumulating in a portion of the reaction mixture. For instance, without agitation, adding a solvent to a solution in order to precipitate a solid material, higher concentration of acetone may accumulate in a portion of the solution causing precipitation of solids in an uneven manner. A gummy form of the solid or a solid with other undesirable character may result. Agitation of the solution while adding the solvent, however, can help prevent uneven distribution of the added solvent and prevent undesirable forms of precipitated solid.
  • Agitation speed can be described in terms of revolutions per minute (RPM).
  • RPM revolutions per minute
  • agitation is greater than about 200 RPM, about 150 RPM, about 100 RPM, or about 50 RPM.
  • agitation is between about 150 to about 250 RPM, about 100 to 200 RPM, 50 to 150 RPM.
  • Agitation is particularly important in heterogeneous mixtures (e.g., slurry).
  • the agitation speed may vary depending on the scale of the reaction volume with smaller reaction volumes having higher RPM than larger reaction volumes. For instance, maximum agitation speed in a production plant may reach about 100 to about 150 RPM. Maximum agitation speed in the laboratory setting may reach over about 200 RPM. In some methods agitation is between about 30 to about 60 RPM.
  • a mixture is formed.
  • the mixture is a homogeneous solution.
  • the mixture is heterogeneous, wherein the mixture comprises more than one phase, for instance a solid phase and a liquid phase.
  • the mixture is a slurry.
  • a portion of the contents of a mixture may undergo phase change over time.
  • a homogenous solution mixture may form solids over time and become a heterogeneous mixture, wherein the mixture comprises a solid and liquid phase.
  • a heterogeneous mixture may become a homogenous solution mixture, for instance when a solid material dissolves into a solvent.
  • the phase change occurs upon a reaction event.
  • a homogenous solution mixture may, upon a reaction event, may become a heterogeneous mixture, and vice versa.
  • the reaction event may be a change in the conditions of the reaction mixture, for instance, cooling or heating, addition of a particular solvent, addition of a solid, or evaporation.
  • polymorph Form 3 which is a polymorph of a monohydrate, bis-mesylate salt of a compound of Formula I, comprising:
  • the method further comprises isolating the polymorph Form 3 produced.
  • the bis-mesylate salt is added to an amount of polymorph Form 3 seeds to convert the bis-mesylate salt to Form 3.
  • the bis-mesylate salt is polymorph Form 7, which is isolated, then further used to make Form 3.
  • the bis-mesylate salt is Form 7, and is not isolated from the reaction mixture.
  • the amount of Form 3 seeds is substantially smaller than the amount of free base used in the method to obtain Form 3, and is also substantially smaller than the amount of Form 3 obtained by the methods described herein.
  • the amount of Form 3 seed added to the bis-mesylate salt is between 0.01 molar % and 5 molar %; between 0.01 molar % and 3 molar %; between 0.01 molar % and 2 molar %; between 0.01 molar % and 1 molar %; between 0.1 molar % and 2 molar %; or between 0.1 molar % and 1 molar %; about 1 molar % of the free base.
  • the amount of Form 3 seeds added to the bis-mesylate salt is between 0.001 and 0.1 weight percent; between 0.01 and 0.1 weight percent; or between 0.01 and 0.08 weight percent; or about 0.015 weight percent of the free base.
  • the amount of methanesulfonic acid is between about 2.0 and about 2.5 molar equivalents; between about 2.0 and about 2.4 molar equivalents; between about 2.0 and about 2.2 molar equivalents; between about 2.0 and about 2.1 molar equivalents; between about 2.0 and about 2.05 molar equivalents; or about 2.05 molar equivalents with respect to one molar equivalent of the compound of Formula I.
  • At least one solvent is added to the mixture.
  • solvents include methanol, ethanol, isopropanol, ethyl acetate, isopropyl acetate, acetone, tetrahydrofuran, toluene, methyl-t-butyl ether, acetonitrile, heptanes, hexanes, water, methyl ethyl ketone, dichloromethane, 2-methyl-tetrahydrofuran, and methyl isobutyl ketone.
  • the solvent is acetone.
  • the solvents are acetone and water.
  • the at least one solvent is an organic solvent. In some embodiments, the at least one solvent is an organic solvent, further comprising water.
  • organic solvents include methanol, ethanol, isopropanol, ethyl acetate, isopropyl acetate, acetone, tetrahydrofuran, toluene, methyl-t-butyl ether, acetonitrile, heptanes, hexanes, methyl ethyl ketone, dichloromethane, 2-methyl-tetrahydrofuran, and methyl isobutyl ketone.
  • the at least one solvent further comprises a protic solvent.
  • a protic solvent include water, methanol, ethanol, isopropanol, propanol, and butanol.
  • the at least one solvent is acetone, further comprising water.
  • the at least one solvent is an organic solvent and water.
  • the concentration of water in the water/organic solvent mixture is from about 1% to about 7.5%; from about 2% to about 5%; from about 4% to about 6%; or is about 5%.
  • the at least one solvent is acetone and water, wherein the concentration of water in the acetone/water solvent mixture is about 1% to about 7.0%; about 2% to about 5%; about 4% to about 6%; or about 5%.
  • the ratio of water to acetone is about 1:15 to about 1:40; about 1:18 to about 1:22; about 1:19; or about 1:38.
  • the method comprises heating the mixture.
  • lower temperatures require less water. For instance, while heating the mixture to 55° C. might require a water concentration of about 5%, heating the mixture to 35° C. might require a water concentration of about 4%.
  • polymorph Form 3 which is a polymorph of a monohydrate, bis-mesylate salt of a compound of Formula I, comprising:
  • polymorph Form 3 seeds, acetone and water to polymorph Form 7 (which is a polymorph of a hydrate, bis-mesylate salt of a compound of Formula I) to form a mixture; and
  • the method further comprises isolating the polymorph Form 3 produced from the mixture.
  • polymorph Form 3 is a polymorph of a monohydrate, bis-mesylate salt of a compound of Formula I, such as a monohydrate of a compound of Formula IA
  • isolating polymorph Form 3 comprising adding an amount of polymorph Form 3 seeds, acetone and water to polymorph Form 7 to form a mixture; and isolating polymorph Form 3 produced, wherein the ratio of water to acetone is about 1:18 to about 1:22; optionally further comprising agitating the mixture, and optionally further comprising heating and cooling the mixture before isolating polymorph Form 3 produced.
  • the mixture is heated to reflux and agitated.
  • heating the mixture results in a faster conversion of Form 7 to Form 3 compared to not heating the mixture.
  • isolating polymorph Form 3 comprises filtering the mixture to obtain solids. In some embodiments, the solids are rinsed with a solvent and then dried.
  • polymorph Form 3 is a polymorph of a monohydrate, bis-mesylate salt of a compound of Formula I, such as a monohydrate of a compound of Formula IA:
  • polymorph Form 3 comprising adding an amount of polymorph Form 3 seeds and at least one solvent to polymorph Form 7 to form a mixture; and isolating polymorph Form 3 produced, wherein polymorph Form 3 has an X-ray diffraction (XRPD) pattern comprising at least two or more of the 2 ⁇ -reflections ( ⁇ 0.2 degrees): 13.8, 16.9, 22.9, and 26.1; and polymorph Form 7 has an X-ray diffraction (XRPD) pattern comprising at least two or more 2 ⁇ -reflections ( ⁇ 0.2 degrees): 4.9, 9.8, and 26.7.
  • XRPD X-ray diffraction
  • polymorph Form 3 which is a polymorph of a monohydrate, bis-mesylate salt of a compound of Formula I:
  • the compound of Formula I comprising adding acetone and water to the compound of Formula I to form a mixture, adding an amount of methanesulfonic acid to the mixture, heating the mixture, adding an amount of polymorph Form 3 seeds to the mixture; cooling the mixture; and recovering the compound of Formula I as polymorph Form 3, wherein the amount of methanesulfonic acid is about 2.05 molar equivalents with respect to one molar equivalent of the compound of Formula I.
  • the compound of Formula I, acetone, and water mixture are under agitation, and methanesulfonic acid is added to the agitated mixture.
  • the mixture is heated to reflux and agitated.
  • polymorph Form 7 which is a polymorph of a hydrate, bis-mesylate salt of a compound of Formula I, comprising:
  • the method further comprises isolating polymorph Form 7 produced.
  • the solvent comprises methanol, ethanol, isopropanol, ethyl acetate, isopropyl acetate, acetone, tetrahydrofuran, toluene, methyl-t-butyl ether, acetonitrile, heptanes, hexanes, water, methyl ethyl ketone, dichloromethane, 2-methyl-tetrahydrofuran, and methyl isobutyl ketone.
  • the solvent comprises acetone.
  • the solvent comprises acetone and water.
  • the solvent is an organic solvent.
  • the solvent is an organic solvent, further comprising water.
  • Non-limiting examples of organic solvents include methanol, ethanol, isopropanol, ethyl acetate, isopropyl acetate, acetone, tetrahydrofuran, toluene, methyl-t-butyl ether, acetonitrile, heptanes, hexanes, methyl ethyl ketone, dichloromethane, 2-methyl-tetrahydrofuran, and methyl isobutyl ketone.
  • the solvent further comprises a protic solvent.
  • Non-limiting examples of a protic solvent include water, methanol, ethanol, isopropanol, propanol, and butanol.
  • the at least one solvent is acetone, further comprising water.
  • the mixture is heated to reflux. In some embodiments, the mixture is heated to a temperature between about 40° C. and about 65° C. In some embodiments, the mixture is heated to a temperature between about 45° C. and about 65° C. In some embodiments, the mixture is heated to a temperature of about 55° C.
  • the heated mixture is cooled to room temperature. In some embodiments, the heated mixture is cooled to a temperature between about 0° C. and about 30° C., or between about 10° C. and about 30° C., or between about 10° C. and about 25° C., or between about 5° C. and about 30° C., or between about 10° C. and about 30° C., or about 10° C. and about 20° C.
  • any formula or structure given herein, including a compound of Formula I and pharmaceutically acceptable salts thereof (including, for example, the mono-mesylate and the bis-mesylate salts), or a hydrate thereof, is also contemplated as an isotopically labeled form of the compounds, or salts, or hydrates thereof.
  • the unlabeled forms of compounds are provided, it is understood that the present disclosure also contemplates isotopically labeled compounds, even though such isotopes are not explicitly depicted.
  • Isotopically labeled compounds, or salts, or hydrates thereof have structures depicted by the formulas given herein except that one or more atoms are replaced by an atom having a selected atomic mass or mass number.
  • isotopes that can be incorporated into compounds of the disclosure include isotopes of hydrogen, carbon, nitrogen, oxygen, phosphorous, fluorine and chlorine, such as, but not limited to 2 H (deuterium, D), 3 H (tritium), 11 C, 13 C, 14 C, 15 N, 18 F, 31 P, 32 P, 35 S, 36 Cl and 125 I.
  • isotopes of hydrogen, carbon, nitrogen, oxygen, phosphorous, fluorine and chlorine such as, but not limited to 2 H (deuterium, D), 3 H (tritium), 11 C, 13 C, 14 C, 15 N, and 35 S may be incorporated into a compound of formula I, including a salt (e.g.
  • a mesylate salt of a compound of formula I, or a hydrate thereof.
  • Various isotopically labeled compounds, or salts, or hydrates thereof of the present disclosure for example those into which radioactive isotopes such as 3 H, 13 C and 14 C are incorporated.
  • Such isotopically labeled compounds or salts thereof may be useful in metabolic studies, reaction kinetic studies, detection or imaging techniques, such as positron emission tomography (PET) or single-photon emission computed tomography (SPECT) including drug or substrate tissue distribution assays or in radioactive treatment of subjects.
  • PET positron emission tomography
  • SPECT single-photon emission computed tomography
  • the disclosure also includes a compound of Formula I and pharmaceutically acceptable salts thereof (including, for example, the mono-mesylate and the bis-mesylate salts), or a hydrate thereof, in which from 1 to n hydrogens attached to a carbon atom is/are replaced by deuterium, in which n is the number of hydrogens in the molecule.
  • Such compounds may exhibit increased resistance to metabolism and are thus useful for increasing the half life of a compound of Formula I, or pharmaceutically acceptable salts thereof, or hydrates thereof (including a bis-mesylate salt of Formula IA or IB, or a hydrate thereof; or a hydrate, bis-mesylate of Formula IC or ID) when administered to a mammal.
  • Deuterium labeled or substituted therapeutic compounds of the disclosure may have improved DMPK (drug metabolism and pharmacokinetics) properties, relating to absorption, distribution, metabolism and excretion (ADME). Substitution with heavier isotopes such as deuterium may afford certain therapeutic advantages resulting from greater metabolic stability, for example increased in vivo half-life, reduced dosage requirements and/or an improvement in therapeutic index.
  • An 18 F labeled compound may be useful for PET or SPECT studies.
  • Isotopically labeled compounds of this disclosure and prodrugs thereof can generally be prepared by carrying out the procedures disclosed in the schemes or in the examples and preparations described below by substituting a readily available isotopically labeled reagent for a non-isotopically labeled reagent. It is understood that deuterium in this context is regarded as a substituent in the compound of Formula I and pharmaceutically acceptable salts thereof (including, for example, the mono-mesylate and the bis-mesylate salts), or hydrates thereof.
  • the concentration of such a heavier isotope, specifically deuterium may be defined by an isotopic enrichment factor.
  • any atom not specifically designated as a particular isotope is meant to represent any stable isotope of that atom.
  • a position is designated specifically as “H” or “hydrogen”, the position is understood to have hydrogen at its natural abundance isotopic composition.
  • any atom specifically designated as a deuterium (D) is meant to represent deuterium.
  • the mesylate salts described herein can be administered as the neat chemical, but it is typical, and preferable, to administer the compound, or salt or hydrate thereof, in the form of a pharmaceutical composition or formulation.
  • pharmaceutical compositions comprising: (i) a bis-mesylate salt of a compound of Formula I, or a hydrate thereof, or polymorph of the foregoing, and (ii) a pharmaceutical carrier, excipient, adjuvant, or vehicle.
  • compositions that comprise polymorph Form 3 and/or Form 7 and a biocompatible pharmaceutical carrier, excipient, adjuvant, or vehicle.
  • the composition can include the bis-mesylate salt of a compound of Formula I, or a hydrate thereof, or polymorphic forms described herein either as the sole active agent or in combination with other agents, such as oligo- or polynucleotides, oligo- or polypeptides, drugs, or hormones mixed with one or more pharmaceutically acceptable carriers or excipients.
  • Carriers, excipients, and other ingredients can be deemed pharmaceutically acceptable insofar as they are compatible with other ingredients of the formulation and not deleterious to the recipient thereof.
  • a pharmaceutical composition comprising: a bis-mesylate salt of a compound of Formula I, or a hydrate thereof; and a pharmaceutical carrier, excipient, adjuvant, or vehicle.
  • a pharmaceutical composition comprising: a hydrate, bis-mesylate salt of a compound of Formula I, and a pharmaceutical carrier, excipient, adjuvant, or vehicle.
  • a pharmaceutical composition comprising: a monohydrate, bis-mesylate salt of a compound of Formula I, and a pharmaceutical carrier, excipient, adjuvant, or vehicle.
  • a pharmaceutical composition comprising: a mesylate salt of Formula IA or IB, or a hydrate thereof; and a pharmaceutical carrier, excipient, adjuvant, or vehicle.
  • a pharmaceutical composition comprising: a hydrate, mesylate salt of Formula IA or IB; and a pharmaceutical carrier, excipient, adjuvant, or vehicle.
  • a pharmaceutical composition comprising polymorph Form 3, and a pharmaceutical acceptable carrier or other excipient.
  • the polymorph Form 3 in the composition is present in excess of other polymorphic forms.
  • the weight ratio of polymorph Form 3 to other polymorph forms in the pharmaceutical composition may be between 85 to 15, 90 to 10, 95 to 5, or 99 to 1. In one embodiment, the weight ratio of polymorph Form 3 to other polymorph forms is between 90:1 and 99:1.
  • carrier refers to diluents, disintegrants, precipitation inhibitors, surfactants, glidants, binders, lubricants, and other excipients and vehicles with which the compound is administered. Carriers are generally described herein and also in “Remington's Pharmaceutical Sciences” by E. W. Martin.
  • compositions can be formulated to contain suitable pharmaceutically acceptable carriers, and optionally can comprise excipients and auxiliaries that facilitate processing of the polymorphic forms described herein into preparations that can be used pharmaceutically.
  • the mode of administration generally determines the nature of the carrier.
  • formulations for parenteral administration can include aqueous solutions of the active compounds in water-soluble form.
  • Carriers suitable for parenteral administration can be selected from among saline, buffered saline, dextrose, water, and other physiologically compatible solutions.
  • Preferred carriers for parenteral administration are physiologically compatible buffers such as Hanks's solution, Ringer's solution, or physiologically buffered saline.
  • penetrants appropriate to the particular barrier to be permeated are used in the formulation.
  • penetrants are generally known in the art.
  • the formulation can include stabilizing materials, such as polyols (e.g., sucrose) and/or surfactants (e.g., nonionic surfactants), and the like.
  • formulations for parenteral use can include dispersions or suspensions of polymorphic forms described herein prepared as appropriate oily injection suspensions.
  • Suitable lipophilic solvents or vehicles include fatty oils, such as sesame oil, and synthetic fatty acid esters, such as ethyl oleate or triglycerides, or liposomes.
  • Aqueous injection suspensions can contain substances that increase the viscosity of the suspension, such as sodium carboxymethylcellulose, sorbitol, dextran, and mixtures thereof.
  • the suspension also can contain suitable stabilizers or agents that increase the solubility of the compounds to allow for the preparation of highly concentrated solutions.
  • Aqueous polymers that provide pH-sensitive solubilization and/or sustained release of the active agent also can be used as coatings or matrix structures, e.g., methacrylic polymers, such as the EUDRAGITTM series available from Rohm America Inc. (Piscataway, N.J.).
  • Emulsions e.g., oil-in-water and water-in-oil dispersions, also can be used, optionally stabilized by an emulsifying agent or dispersant (surface active materials; surfactants).
  • Suspensions can contain suspending agents such as ethoxylated isostearyl alcohols, polyoxyethlyene sorbitol and sorbitan esters, microcrystalline cellulose, aluminum metahydroxide, bentonite, agar-agar, gum tragacanth, and mixtures thereof.
  • suspending agents such as ethoxylated isostearyl alcohols, polyoxyethlyene sorbitol and sorbitan esters, microcrystalline cellulose, aluminum metahydroxide, bentonite, agar-agar, gum tragacanth, and mixtures thereof.
  • Liposomes containing the polymorphic forms described herein also can be employed for parenteral administration.
  • Liposomes generally are derived from phospholipids or other lipid substances.
  • the compositions in liposome form also can contain other ingredients, such as stabilizers, preservatives, excipients, and the like.
  • Preferred lipids include phospholipids and phosphatidyl cholines (lecithins), both natural and synthetic. Methods of forming liposomes are known in the art. See, e.g., Prescott (Ed.), Methods in Cell Biology, Vol. XIV, p. 33, Academic Press, New York (1976).
  • the polymorph, or composition thereof, disclosed herein is formulated for oral administration using pharmaceutically acceptable carriers well known in the art.
  • Preparations formulated for oral administration can be in the form of tablets, pills, capsules, cachets, dragees, lozenges, liquids, gels, syrups, slurries, elixirs, suspensions, or powders.
  • pharmaceutical preparations for oral use can be obtained by combining the active compounds with a solid excipient, optionally grinding the resulting mixture, and processing the mixture of granules, after adding suitable auxiliaries if desired, to obtain tablets or dragee cores.
  • Oral formulations can employ liquid carriers similar in type to those described for parenteral use, e.g., buffered aqueous solutions, suspensions, and the like.
  • Preferred oral formulations include tablets, dragees, and gelatin capsules. These preparations can contain one or more excipients, which include, without limitation: a) diluents, such as microcrystalline cellulose and sugars, including lactose, dextrose, sucrose, mannitol, or sorbitol; b) binders, such as sodium starch glycolate, croscarmellose sodium, magnesium aluminum silicate, starch from corn, wheat, rice, potato, etc.; c) cellulose materials, such as methylcellulose, hydroxypropylmethyl cellulose, and sodium carboxymethylcellulose, polyvinylpyrrolidone, gums, such as gum arabic and gum tragacanth, and proteins, such as gelatin and collagen; d) disintegrating or solubilizing agents such as cross-linked polyvinyl pyrrolidone, starches, agar, alginic acid or a salt thereof, such as sodium alginate, or effervescent compositions;
  • Examples of preferred carriers include, but are not limited to, aluminum monostearate, aluminum stearate, carboxymethylcellulose, carboxymethylcellulose sodium, crospovidone, glyceryl isostearate, glyceryl monostearate, hydroxyethylcellulose, hydroxymethylcellulose, hydroxyoctacosanyl hydroxystearate, hydroxypropylcellulose, hydroxypropylmethylcellulose, lactose, lactose monohydrate, magnesium stearate, mannitol, microcrystalline cellulose, poloxamer 124, poloxamer 181, poloxamer 182, poloxamer 188, poloxamer 237, poloxamer 407, povidone, silicon dioxide, colloidal silicon dioxide, silicone, silicone adhesive 4102, and silicone emulsion. It should be understood, however, that the carriers selected for the pharmaceutical compositions provided in the present disclosure, and the amounts of such carriers in the composition, may vary depending on the method of formulation (e.g., dry granulation formulation, solid dis
  • the pharmaceutical composition comprises Form 3, and at least one pharmaceutically acceptable carrier selected from the group consisting of hydroxypropylmethylcellulose, mannitol, crospovidone, poloxamer, colloidal silicon dioxide, microcrystalline cellulose, magnesium stearate, and any mixtures thereof.
  • the pharmaceutical composition comprises polymorph Form 3, hydroxypropylmethylcellulose, and at least one additionally pharmaceutically acceptable carrier selected from the group consisting of mannitol, crospovidone, poloxamer, colloidal silicon dioxide, microcrystalline cellulose, magnesium stearate, and any mixtures thereof.
  • the pharmaceutically acceptable carriers described above may perform one or more different functions in a given formulation, and may fall within one or more functional classes of carriers (e.g., disintegrants, lubricants, diluents).
  • the pharmaceutical composition may comprise one or more additional carriers to improve flow, compression, hardness, taste, and tablet performance.
  • the pharmaceutical composition comprises a) about 34% w/w of a mesylate salt (including, for example, a mono-mesylate or bis-mesylate salt) of a compound of Formula I; b) about 15% w/w HPMC; c) about 22% w/w mannitol; d) about 10% w/w crospovidone; and e) about 1% w/w to about 3% w/w poloxamer.
  • a mesylate salt including, for example, a mono-mesylate or bis-mesylate salt
  • the pharmaceutical composition comprises: a) about 34% w/w of a bis-mesylate salt of a compound of Formula I, or a hydrate thereof; b) about 15% w/w HPMC; c) about 22% w/w mannitol; d) about 10% w/w crospovidone; and e) about 1% w/w to about 3% w/w poloxamer.
  • the pharmaceutical composition comprises: a) about 34% w/w of a monohydrate, bis-mesylate salt of a compound of Formula I; b) about 15% w/w HPMC; c) about 22% w/w mannitol; d) about 10% w/w crospovidone; and e) about 1% w/w to about 3% w/w poloxamer.
  • the pharmaceutical composition comprises: a) about 34% w/w of polymorph Form 3, polymorph Form 7, or a combination thereof; b) about 15% w/w HPMC; c) about 22% w/w mannitol; d) about 10% w/w crospovidone; and e) about 1% w/w to about 3% w/w poloxamer.
  • the method comprises administering the pharmaceutical composition to an individual in need thereof in an amount sufficient to inhibit Syk activity.
  • the method can be employed to treat subjects (e.g., humans) suffering from, or subject to, a condition whose symptoms or pathology is mediated by Syk expression or activity.
  • a method of treating a human in need thereof comprising administering a hydrate, bis-mesylate salt of a compound of Formula I to the human.
  • a method of treating a human in need thereof comprising administering a monohydrate, bis-mesylate salt of a compound of Formula I to the human.
  • Treatment or “treating” is an approach for obtaining beneficial or desired results including clinical results.
  • beneficial or desired clinical results may include one or more of the following:
  • a) inhibiting the disease or condition e.g., decreasing one or more symptoms resulting from the disease or condition, and/or diminishing the extent of the disease or condition
  • clinical symptoms e.g., ameliorating the disease state, providing partial or total remission of the disease or condition, enhancing effect of another medication, delaying the progression of the disease, increasing the quality of life, and/or prolonging survival.
  • Prevention means any treatment of a disease or condition that causes the clinical symptoms of the disease or condition not to develop.
  • Compounds may, in some embodiments, be administered to a subject (including a human) who is at risk or has a family history of the disease or condition.
  • Subject refers to an animal, such as a mammal (including a human), that has been or will be the object of treatment, observation or experiment. The methods described herein may be useful in human therapy and/or veterinary applications.
  • the subject is a mammal. In one embodiment, the subject is a human.
  • terapéuticaally effective amount of the pharmaceutical composition means an amount sufficient to effect treatment when administered to a subject, to provide a therapeutic benefit such as amelioration of symptoms or slowing of disease progression.
  • a therapeutically effective amount may be an amount sufficient to decrease a symptom of a disease or condition responsive to inhibition of Syk activity.
  • the therapeutically effective amount may vary depending on the subject, and disease or condition being treated, the weight and age of the subject, the severity of the disease or condition, and the manner of administering, which can readily be determined by one or ordinary skill in the art.
  • inhibitors indicates a decrease in the baseline activity of a biological activity or process “Inhibition of activity of Syk activity” refers to a decrease in activity of Syk as a direct or indirect response to the presence of the pharmaceutical composition, relative to the activity of Syk in the absence of such pharmaceutical composition. In some embodiments, the inhibition of Syk activity may be compared in the same subject prior to treatment, or other subjects not receiving the treatment.
  • a bis-mesylate salt of a compound of Formula I (including polymorphs of such bis-mesylate salt, such as Form 3 and/or Form 7), or a hydrate thereof, and compositions thereof described herein are used for treating a subject having cancer, an allergic disorder and/or an autoimmune and/or inflammatory disease, and/or an acute inflammatory reaction.
  • the pharmaceutical compositions provided in the present disclosure may be used in the treatment of cancer.
  • the polymorphs and compositions thereof described herein can be employed in methods of inhibiting the growth or proliferation of cancer cells of hematopoietic origin, such as cancer cells.
  • the cancer cells are of lymphoid origin, and in specific embodiments, the cancer cells are related to or derived from B lymphocytes or B lymphocyte progenitors.
  • lymphomas e.g., malignant neoplasms of lymphoid and reticuloendothelial tissues, such as Burkitt's lymphoma, Hodgkins' lymphoma, non-Hodgkins' lymphomas, lymphocytic lymphomas); multiple myelomas; leukemias (e.g., lymphocytic leukemias, chronic myeloid (myelogenous) leukemias).
  • Other cancer cells of hematopoietic origin or otherwise, that express spleen tyrosine kinase (Syk) also can be treated by administration of the polymorphs and compositions thereof described herein.
  • the cancer is leukemia or lymphoma.
  • the cancer is acute lymphocytic leukemia (ALL), acute myeloid leukemia (AML), chronic lymphocytic leukemia (CLL), small lymphocytic lymphoma (SLL), myelodysplastic syndrome (MDS), myeloproliferative disease (MPD), chronic myeloid leukemia (CML), multiple myeloma (MM), indolent non-Hodgkin's lymphoma (iNHL), refractory iNHL, non-Hodgkin's lymphoma (NHL), mantle cell lymphoma (MCL), follicular lymphoma (FL), Waldestrom's macroglobulinemia (WM), T-cell lymphoma, B-cell lymphoma, diffuse large B-cell lymphoma (DLBCL), lymphoplasmacytic lymphoma (LPL), and marginal zone lympho
  • ALL acute lymphocytic leukemia
  • the cancer is T-cell acute lymphoblastic leukemia (T-ALL), or B-cell acute lymphoblastic leukemia (B-ALL).
  • T-ALL T-cell acute lymphoblastic leukemia
  • B-ALL B-cell acute lymphoblastic leukemia
  • the non-Hodgkin lymphoma encompasses the indolent B-cell diseases that include, for example, follicular lymphoma, lymphoplasmacytic lymphoma, Waldenstrom macroglobulinemia, and marginal zone lymphoma, as well as the aggressive lymphomas that include, for example, Burkitt lymphoma, diffuse large B-cell lymphoma (DLBCL) and mantle cell lymphoma (MCL).
  • the cancer is indolent non-Hodgkin's lymphoma (iNHL).
  • the cancer is non-FL iNHL.
  • the cancer is a hematologic malignancy.
  • the hematologic malignancy is leukemia (e.g., chronic lymphocytic leukemia) or lymphoma (e.g., non-Hodgkin's lymphoma).
  • the cancer is MCL, DLBCL, iNHL, FL, MZL, LPL, SLL, or WM.
  • the cancer is CLL, MCL, DLBCL, iNHL (including, for example, non-FL iNHL), or FL.
  • the cancer is a solid tumor cancer (or solid cancer tumor).
  • the cancer is a solid tumor and expresses spleen tyrosine kinase (Syk) activity.
  • the solid tumor cancer is selected from the group consisting of pancreatic cancer, lung cancer, colon cancer, colo-rectal cancer, breast cancer, esophageal cancer, adenocarcinoma, hepatocellular cancer.
  • the solid tumor cancer is selected from the group consisting of pancreatic cancer, lung cancer, colorectal cancer, ovarian cancer, and hepatocellular cancer.
  • any of the methods of treatment provided herein may be used to treat cancer at an advanced stage. Any of the methods of treatment provided herein may be used to treat cancer at locally advanced stage. Any of the methods of treatment provided herein may be used to treat early stage cancer. Any of the methods of treatment provided herein may be used to treat cancer in remission. In some of the embodiments of any of the methods of treatment provided herein, the cancer has reoccurred after remission. In some embodiments of any of the methods of treatment provided herein, the cancer is progressive cancer.
  • the conditions and diseases that can be affected using the compounds and the compositions described herein include, but are not limited to: allergic disorders, including but not limited to eczema, allergic rhinitis or coryza, hay fever, bronchial asthma, urticaria (hives) and food allergies, and other atopic conditions; autoimmune and/or inflammatory diseases, including but not limited to psoriasis, ulcerative colitis, Crohn's disease, irritable bowel syndrome, Sjogren's disease, tissue graft rejection, and hyperacute rejection of transplanted organs, asthma, systemic lupus erythematosus (and associated glomerulonephritis), dermatomyositis, multiple sclerosis, scleroderma, vasculitis (ANCA-associated and other vasculitides), autoimmune hemolytic and thrombocytopenic states, Goodpasture's syndrome (and associated glomerulonephritis and pulmonary hemorrhage
  • an autoimmune disease includes asthma, rheumatoid arthritis, multiple sclerosis, and lupus.
  • the pharmaceutical compositions provided in the present disclosure may be administered to the individual as unit dosage, for example in the form of a tablet.
  • a mesylate salt including, for example, a mono-mesylate or bis-mesylate salt
  • a hydrate thereof is administered in the form a tablet.
  • polymorph Form 3 which is a polymorph of a monohydrate, bis-mesylate salt of a compound of Formula I (e.g., polymorph of a monohydrate of the bis-mesylate salt of Formula IA) is used in the spray dry preparation of the unit dosage, wherein the final unit dosage does not substantially contain the polymorph Form 3.
  • Any of the methods of treatment provided may be used to treat a subject who has been diagnosed with or is suspected of having a cancer, an allergic disorder and/or an autoimmune and/or inflammatory disease, and/or an acute inflammatory reaction.
  • the subject is a human who is at risk of developing a cancer (e.g., a human who is genetically or otherwise predisposed to developing a cancer) and who has or has not been diagnosed with the cancer.
  • an “at risk” subject is a subject who is at risk of developing cancer (e.g., a hematologic malignancy).
  • the subject may or may not have detectable disease, and may or may not have displayed detectable disease prior to the treatment methods described herein.
  • An at risk subject may have one or more so-called risk factors, which are measurable parameters that correlate with development of cancer, such as described herein.
  • a subject having one or more of these risk factors has a higher probability of developing cancer than an individual without these risk factor(s).
  • risk factors may include, for example, age, sex, race, diet, history of previous disease, presence of precursor disease, genetic (e.g., hereditary) considerations, and environmental exposure.
  • a subject at risk for cancer includes, for example, a subject whose relatives have experienced this disease, and those whose risk is determined by analysis of genetic or biochemical markers. Prior history of having cancer may also be a risk factor for instances of cancer recurrence.
  • a subject e.g., a human
  • one or more symptoms associated with cancer e.g., a hematologic malignancy
  • the subject is at an early stage of cancer. In other embodiments, the subject is at an advanced stage of cancer.
  • the subject e.g., a human
  • the human has a solid cancer tumor which expresses Syk.
  • the human has a 17p deletion, a TP53 mutation, NOTCH1, a SF3B1 mutation, a 11q deletion, or any combination thereof.
  • the human has a 17p deletion, a TP53 mutation, or a combination thereof.
  • the human has NOTCH1, a SF3B1 mutation, a 11q deletion, or any combination thereof.
  • a bis-mesylate salt of a compound of Formula I (including polymorphs of such bis-mesylate salt, such as Form 3 and/or Form 7), or a hydrate thereof, and compositions described herein is administered before, during, or after administration of chemotherapy, radiotherapy, immunotherapy, and/or surgery.
  • a subject e.g., a human who is “refractory” to a cancer treatment or who is in “relapse” after treatment for cancer (e.g., a hematologic malignancy).
  • a subject “refractory” to an anti-cancer therapy means they do not respond to the particular treatment, also referred to as resistant.
  • the cancer may be resistant to treatment from the beginning of treatment, or may become resistant during the course of treatment, for example after the treatment has shown some effect on the cancer, but not enough to be considered a remission or partial remission.
  • a subject in “relapse” means that the cancer has returned or the signs and symptoms of cancer have returned after a period of improvement, e.g. after a treatment has shown effective reduction in the cancer, such as after a subject is in remission or partial remission.
  • the subject may be a human who is (i) refractory to at least one anti-cancer therapy, or (ii) in relapse after treatment with at least one anti-cancer therapy, or both (i) and (ii).
  • the subject is refractory to at least two, at least three, or at least four anti-cancer therapy (including, for example, standard or experimental chemotherapies).
  • the subject is a human who is (i) refractory to, and/or (ii) in relapse after treatment with at least one therapy for chronic lymphocytic leukemia (CLL), mantle cell lymphoma (MCL), diffuse large B-cell lymphoma (DLBCL), follicular lymphoma (FL), or non-FL indolent non-Hodgkin's lymphoma (including, for example, lymphoplasmacytic lymphoma/Waldestrom's macroglobulinemia (LPL/WM), small lymphocytic lymphoma (SLL), and marginal zone lymphoma (MZL)).
  • CLL chronic lymphocytic leukemia
  • MCL mantle cell lymphoma
  • DLBCL diffuse large B-cell lymphoma
  • FL follicular lymphoma
  • non-FL indolent non-Hodgkin's lymphoma including, for example, lymphoplasmacytic lymphoma/Wal
  • the subject is a human who (i) is refractory to, and/or (ii) is in relapse after treatment with, and/or (iii) has prior exposure to at least one therapy for a non-FL indolent non-Hodgkin's lymphoma.
  • the non-FL indolent non-Hodgkin's lymphoma is lymphoplasmacytic lymphoma/Waldestrom's macroglobulinemia (LPL/WM), small lymphocytic lymphoma (SLL), or marginal zone lymphoma (MZL))
  • the subject is a human who (i) is refractory to, and/or (ii) is in relapse after treatment with, and/or (iii) has prior exposure to at least one therapy for follicular lymphoma (FL).
  • the subject is a human who (i) is refractory to, and/or (ii) is in relapse after treatment with, and/or (iii) has prior exposure to at least one therapy for diffuse large B-cell lymphoma (DLBCL).
  • the subject is a human who (i) is refractory to, and/or (ii) is in relapse after treatment with, and/or (iii) has prior exposure to at least one therapy for mantle cell lymphoma (MCL).
  • MCL mantle cell lymphoma
  • the subject is a human who (i) is refractory to, and/or (ii) is in relapse after treatment with, and/or (iii) has prior exposure to at least one therapy for chronic lymphocytic leukemia (CLL).
  • the subject is a human who (i) is refractory to, and/or (ii) is in relapse after treatment with, and/or (iii) has prior exposure to a phosphatidylinositol 3-kinase (PI3K) inhibitor, a bruton tyrosine kinase (BTK) inhibitor, or a B-cell receptor (BCR) treatment for chronic lymphocytic leukemia (CLL).
  • PI3K phosphatidylinositol 3-kinase
  • BTK bruton tyrosine kinase
  • BCR B-cell receptor
  • the subject is refractory to at least one, at least two, at least three, or at least four anti-cancer therapy (including, for example, standard or experimental chemotherapy) selected from fludarabine, rituximab, obinutuzumab, alkylating agents, alemtuzumab and other chemotherapy treatments such as CHOP (cyclophosphamide, doxorubicin, vincristine, prednisone); R-CHOP (rituximab-CHOP); hyperCVAD (hyperfractionated cyclophosphamide, vincristine, doxorubicin, dexamethasone, methotrexate, cytarabine); R-hyperCVAD (rituximab-hyperCVAD); FCM (fludarabine, cyclophosphamide, mitoxantrone); R-FCM (rituximab, fludarabine, cyclophosphamide, mitoxantrone), R-FC
  • lymphomas are reviewed in Cheson, B. D., Leonard, J. P., “Monoclonal Antibody Therapy for B-Cell Non-Hodgkin's Lymphoma” The New England Journal of Medicine 2008, 359(6), p. 613-626; and Wierda, W. G., “Current and Investigational Therapies for Patients with CLL” Hematology 2006, p. 285-294. Lymphoma incidence patterns in the United States is profiled in Morton, L. M., et al. “Lymphoma Incidence Patterns by WHO Subtype in the United States, 1992-2001 ” Blood 2006, 107(1), p. 265-276.
  • treatment of non-Hodgkin's lymphomas include the use of monoclonal antibodies, standard chemotherapy approaches (e.g., CHOP, CVP, FCM, MCP, and the like), radioimmunotherapy, and combinations thereof, especially integration of an antibody therapy with chemotherapy.
  • standard chemotherapy approaches e.g., CHOP, CVP, FCM, MCP, and the like
  • radioimmunotherapy e.g., radioimmunotherapy
  • unconjugated monoclonal antibodies for Non-Hodgkin's lymphoma/B-cell cancers include rituximab, alemtuzumab, human or humanized anti-CD20 antibodies, lumiliximab, anti-TRAIL, bevacizumab, galiximab, epratuzumab, SGN-40, and anti-CD74.
  • Examples of experimental antibody agents used in treatment of Non-Hodgkin's lymphoma/B-cell cancers include ofatumumab, ha20, PRO131921, alemtuzumab, galiximab, SGN-40, CHIR-12.12, epratuzumab, lumiliximab, apolizumab, milatuzumab, and bevacizumab.
  • Non-Hodgkin's lymphoma/B-cell cancers examples include CHOP (cyclophosphamide, doxorubicin, vincristine, prednisone), FCM (fludarabine, cyclophosphamide, mitoxantrone), CVP (cyclophosphamide, vincristine and prednisone), MCP (mitoxantrone, chlorambucil, and prednisolone), R-CHOP (rituximab plus CHOP), R-FCM (rituximab plus FCM), R-CVP (rituximab plus CVP), and R-MCP (R-MCP).
  • CHOP cyclophosphamide, doxorubicin, vincristine, prednisone
  • FCM fludarabine, cyclophosphamide, mitoxantrone
  • CVP cyclophosphamide, vincristine and prednisone
  • radioimmunotherapy for Non-Hodgkin's lymphoma/B-cell cancers examples include yttrium-90-labeled ibritumomab tiuxetan, and iodine-131-labeled tositumomab.
  • therapeutic treatments for mantle cell lymphoma include combination chemotherapies such as CHOP (cyclophosphamide, doxorubicin, vincristine, prednisone), hyperCVAD (hyperfractionated cyclophosphamide, vincristine, doxorubicin, dexamethasone, methotrexate, cytarabine) and FCM (fludarabine, cyclophosphamide, mitoxantrone).
  • CHOP cyclophosphamide, doxorubicin, vincristine, prednisone
  • hyperCVAD hyperfractionated cyclophosphamide, vincristine, doxorubicin, dexamethasone, methotrexate, cytarabine
  • FCM fludarabine, cyclophosphamide, mitoxantrone
  • these regimens can be supplemented with the monoclonal antibody rituximab (Rituxan
  • ⁇ approaches include combining any of the abovementioned therapies with stem cell transplantation or treatment with ICE (iphosphamide, carboplatin and etoposide).
  • ICE iphosphamide, carboplatin and etoposide.
  • Other approaches to treating mantle cell lymphoma includes immunotherapy such as using monoclonal antibodies like Rituximab (Rituxan).
  • Rituximab can be used for treating indolent B-cell cancers, including marginal-zone lymphoma, WM, CLL and small lymphocytic lymphoma.
  • a modified approach is radioimmunotherapy, wherein a monoclonal antibody is combined with a radioisotope particle, such as Iodine-131 tositumomab (Bexxar®) and Yttrium-90 ibritumomab tiuxetan)(Zevalin®).
  • Bexxar® is used in sequential treatment with CHOP.
  • Another immunotherapy example includes using cancer vaccines, which is based upon the genetic makeup of an individual subject's tumor.
  • a lymphoma vaccine example is GTOP-99) (MyVax®).
  • mantle cell lymphoma includes autologous stem cell transplantation coupled with high-dose chemotherapy, or treating mantle cell lymphoma includes administering proteasome inhibitors, such as Velcade® (bortezomib or PS-341), or antiangiogenesis agents, such as thalidomide, especially in combination with Rituxan.
  • proteasome inhibitors such as Velcade® (bortezomib or PS-341)
  • antiangiogenesis agents such as thalidomide
  • Another treatment approach is administering drugs that lead to the degradation of Bcl-2 protein and increase cancer cell sensitivity to chemotherapy, such as oblimersen (Genasense) in combination with other chemotherapeutic agents.
  • Another treatment approach includes administering mTOR inhibitors, which can lead to inhibition of cell growth and even cell death; a non-limiting example is Temsirolimus (CCI-779), and Temsirolimus in combination with Rituxan®, Velcade® or other chemotherapeutic agents.
  • mTOR inhibitors which can lead to inhibition of cell growth and even cell death
  • a non-limiting example is Temsirolimus (CCI-779), and Temsirolimus in combination with Rituxan®, Velcade® or other chemotherapeutic agents.
  • WM Waldenstrom's Macroglobulinemia
  • examples of other therapeutic agents used to treat Waldenstrom's Macroglobulinemia include perifosine, bortezomib)(Velcade®), rituximab, sildenafil citrate)(Viagra®), CC-5103, thalidomide, epratuzumab (hLL2-anti-CD22 humanized antibody), simvastatin, enzastaurin, campath-1H, dexamethasone, DT PACE, oblimersen, antineoplaston A10, antineoplaston AS2-1, alemtuzumab, beta alethine, cyclophosphamide, doxorubicin hydrochloride, prednisone, vincristine sulfate, fludarabine, filgrastim, melphalan, recombinant interferon alfa, carmustine, cisplatin, cyclopho
  • Examples of therapeutic procedures used to treat WM include peripheral blood stem cell transplantation, autologous hematopoietic stem cell transplantation, autologous bone marrow transplantation, antibody therapy, biological therapy, enzyme inhibitor therapy, total body irradiation, infusion of stem cells, bone marrow ablation with stem cell support, in vitro-treated peripheral blood stem cell transplantation, umbilical cord blood transplantation, immunoenzyme technique, pharmacological study, low-LET cobalt-60 gamma ray therapy, bleomycin, conventional surgery, radiation therapy, and nonmyeloablative allogeneic hematopoietic stem cell transplantation.
  • Examples of other therapeutic agents used to treat diffuse large B-cell lymphoma (DLBCL) drug therapies include cyclophosphamide, doxorubicin, vincristine, prednisone, anti-CD20 monoclonal antibodies, etoposide, bleomycin, many of the agents listed for Waldenstrom's, and any combination thereof, such as ICE and R-ICE.
  • CLL chronic lymphocytic leukemia
  • examples of other therapeutic agents used to treat chronic lymphocytic leukemia include Chlorambucil (Leukeran), Cyclophosphamide (Cyloxan, Endoxan, Endoxana, Cyclostin), Fludarabine (Fludara), Pentstatin (Nipent), Cladribine (Leustarin), Doxorubicin (Adriamycin®, Adriblastine), Vincristine (Oncovin), Prednisone, Prednisolone, Alemtuzumab (Campath, MabCampath), many of the agents listed for Waldenstrom's, and combination chemotherapy and chemoimmunotherapy, including the common combination regimen: CVP (cyclophosphamide, vincristine, prednisone); R-CVP (rituximab-CVP); ICE (iphosphamide, carboplatin, etoposide);
  • a method of sensitizing a subject e.g., a human who is (i) refractory to at least one chemotherapy treatment, or (ii) in relapse after treatment with chemotherapy, or both (i) and (ii), wherein the method comprises administering a bis-mesylate salt of a compound of Formula I (including polymorphs of such bis-mesylate salt, such as Form 3 and/or Form 7), or a hydrate thereof, or a pharmaceutical composition thereof, to the subject.
  • a bis-mesylate salt of a compound of Formula I including polymorphs of such bis-mesylate salt, such as Form 3 and/or Form 7
  • a subject who is sensitized is a subject who is responsive to the treatment involving administration of a bis-mesylate salt of a compound of Formula I (including polymorphs of such bis-mesylate salt, such as Form 3 and/or Form 7), or a hydrate thereof, and compositions thereof described herein, or who has not developed resistance to such treatment.
  • a bis-mesylate salt of a compound of Formula I including polymorphs of such bis-mesylate salt, such as Form 3 and/or Form 7
  • compositions thereof described herein or who has not developed resistance to such treatment.
  • a subject e.g., a human
  • comorbidity to cancer is a disease that occurs at the same time as the cancer.
  • a subject e.g., a human
  • CLL chronic lymphocytic leukemia
  • Many subjects with CLL will have one or more other diseases, for example diseases affecting the blood pressure system, vascular and heart systems, endocrine and metabolic systems, genitourinary system, musculoskeletal system, respiratory system, neurological system, upper and lower gastrointestinal systems, psychiatric system, ear, nose and throat systems, renal system, or liver system.
  • Specific morbidities of CLL include, but are not limited to, one or more other cancers (e.g.
  • transient ischemic attack stroke
  • chronic obstructive pulmonary disease joint disease
  • peptic ulcer inflammatory bowel disease
  • psychiatric illness thyroid disease
  • benign prostate hyperplasia diabetes mellitus
  • osteoarthritis Ster-Hoang et al., Journal of Cancer Therapy, 2013; 4:1321-1329
  • a method of treating a comorbidity of CLL in a subject comprising administering a compound of Formula I, or a pharmaceutically acceptable salt thereof, or a pharmaceutical composition thereof, to the subject.
  • the comorbidity is selected from the group consisting of one or more other cancers (e.g.
  • cerebrovascular disease e.g. transient ischemic attack, stroke
  • chronic obstructive pulmonary disease e.g. transient ischemic attack, stroke
  • chronic obstructive pulmonary disease e.g. transient ischemic attack, stroke
  • chronic obstructive pulmonary disease e.g. transient ischemic attack, stroke
  • a subject e.g., a human
  • the mesylate salt including, for example, a bis-mesylate salt
  • a subject e.g., a human
  • the pharmaceutical composition provided in the present disclosure administered to a subject is given to a subject (e.g., a human) in combination with one or more additional therapeutic agents or other therapies.
  • Both monotherapy and combination therapies are intended and described for use in the methods detailed herein, such as in a method of treating any of the diseases or conditions detailed herein and for use with any subject detailed herein.
  • a method of treating cancer, an allergic disorder and/or an autoimmune and/or inflammatory disease, and/or an acute inflammatory reaction comprises administering to a subject in need thereof an effective amount of a bis-mesylate salt of a compound of Formula I (including polymorphs of such bis-mesylate salt, such as Form 3 and/or Form 7), or a hydrate thereof, wherein the subject is not undergoing therapy for the same disease or condition with another agent or procedure.
  • a bis-mesylate salt of a compound of Formula I including polymorphs of such bis-mesylate salt, such as Form 3 and/or Form 7
  • the bis-mesylate salt of a compound of Formula I (including polymorphs of such bis-mesylate salt, such as Form 3 and/or Form 7), or a hydrate thereof, is administered as a monotherapy to the subject who has been diagnosed with or is suspected of having a cancer
  • the subject may be a human who is (i) refractory to at least one anti-cancer therapy, or (ii) in relapse after treatment with at least one anti-cancer therapy, or both (i) and (ii).
  • the subject is refractory to at least two, at least three, or at least four anti-cancer therapy (including, for example, standard or experimental chemotherapies).
  • the subject may be a human who is (i) refractory to a therapy using an anti-CD20 antibody, an alkylating agent (e.g., bendamustine), a purine analog (e.g., fludarabine), an anthracycline, or any combination thereof; (ii) in relapse after treatment with an anti-CD20 antibody, an alkylating agent (e.g., bendamustine), a purine analog (e.g., fludarabine), an anthracycline, or any combination thereof, or both (i) and (ii).
  • an alkylating agent e.g., bendamustine
  • a purine analog e.g., fludarabine
  • an anthracycline e.g., fludarabine
  • a human subject who is refractory to at least one anti-cancer therapy and/or is in relapse after treatment with at least one anti-cancer therapy, as described above, may have undergone one or more prior therapies.
  • such subjects have undergone one, two, three, or four, or at least one, at least two, at least three, at least four, or at least five, or between one and ten, between one and nine, between one and eight, between one and seven, between one and six, between one and five, or between one and four, anti-cancer therapies prior to treatment using the methods described herein (e.g., prior to the administration of the compound of Formula I, or a pharmaceutically acceptable salt thereof, as a monotherapy).
  • a subject e.g. a human
  • the compound of Formula I, or a pharmaceutically acceptable salt thereof may also undergo one or more other therapies that are not anti-cancer therapies.
  • a method of treating a comorbidity of a cancer including but not limited to CLL, in a subject (e.g., a human) who has been diagnosed with cancer, e.g. CLL, wherein the method comprises administering a therapy to treat the comorbidity in combination with of a bis-mesylate salt of a compound of Formula I (including polymorphs of such bis-mesylate salt, such as Form 3 and/or Form 7), or a hydrate thereof, or a pharmaceutical composition thereof, to the subject.
  • the comorbidity is selected from the group consisting of one or more other cancers (e.g.
  • cerebrovascular disease e.g. transient ischemic attack, stroke
  • chronic obstructive pulmonary disease e.g. transient ischemic attack, stroke
  • chronic obstructive pulmonary disease e.g. transient ischemic attack, stroke
  • chronic obstructive pulmonary disease e.g. transient ischemic attack, stroke
  • a method of treating cancer, an allergic disorder and/or an autoimmune and/or inflammatory disease, and/or an acute inflammatory reaction comprises administering to a subject (e.g., a human) in need thereof an effective amount of the pharmaceutical composition described herein, together with one or more additional therapies (e.g., one or more additional therapeutic agents), which can be useful for treating a cancer, an allergic disorder and/or an autoimmune and/or inflammatory disease, and/or an acute inflammatory reaction.
  • additional therapies e.g., one or more additional therapeutic agents
  • a method of treating cancer, an allergic disorder and/or an autoimmune and/or inflammatory disease, and/or an acute inflammatory reaction comprises administering to a subject in need thereof an effective amount of a bis-mesylate salt of a compound of Formula I (including polymorphs of such bis-mesylate salt, such as Form 3 and/or Form 7), or a hydrate thereof, together with a second active agent, which can be useful for treating a cancer, an allergic disorder and/or an autoimmune and/or inflammatory disease, and/or an acute inflammatory reaction.
  • the second agent may be an anti-inflammatory agent.
  • Treatment with the second active agent may be prior to, concomitant with, or following treatment with a bis-mesylate salt of a compound of Formula I (including polymorphs of such bis-mesylate salt, such as Form 3 and/or Form 7), or a hydrate thereof.
  • a bis-mesylate salt of a compound of Formula I is combined with another active agent in a single dosage form.
  • a bis-mesylate salt of a compound of Formula I (including polymorphs of such bis-mesylate salt, such as Form 3 and/or Form 7), or a hydrate thereof, is administered to a subject (e.g., a human) who has been diagnosed with or is suspected of having a cancer is given to the subject in combination with one or more additional therapies, including one or more of the anti-cancer therapies described above.
  • the method for treating cancer in a subject comprises administering to the subject a therapeutically effective amount of a bis-mesylate salt of a compound of Formula I (including polymorphs of such bis-mesylate salt, such as Form 3 and/or Form 7), or a hydrate thereof, or a pharmaceutical composition thereof, together with one or more additional therapies, which can be useful for treating the cancer.
  • the one or more additional therapies may involve the administration of one or more therapeutic agents.
  • a method of treating cancer in a human in need thereof comprising administering a bis-mesylate salt of a compound of Formula I (including polymorphs of such bis-mesylate salt, such as Form 3 and/or Form 7), or a hydrate thereof, and an additional therapeutic agent to the human.
  • a method of treating cancer in a human in need thereof comprising administering a hydrate, bis-mesylate salt of a compound of Formula I to the human, and an additional therapeutic agent to the human.
  • a method of treating cancer in a human in need thereof comprising administering a monohydrate, bis-mesylate salt of a compound of Formula I to the human, and an additional therapeutic agent to the human.
  • a method of treating cancer in a human in need thereof comprising administering a hydrate, mesylate salt of Formula IA or IB to the human, and an additional therapeutic agent to the human.
  • a method of treating cancer in a human in need thereof comprising administering polymorph Form 3 to the human, and an additional therapeutic agent to the human.
  • a method of treating cancer in a human in need thereof comprising administering polymorph Form 7 to the human, and an additional therapeutic agent to the human.
  • the treatment of cancer may include, for example, leukemia, lymphoma and solid-cell tumors.
  • the additional therapeutic agent may be one or more agents.
  • the one or more additional therapeutic agent may be a phosphatidylinositol 3-kinase (PI3K) inhibitor, including for example, Compounds A, B, C and D, whose structures are provided below.
  • PI3K phosphatidylinositol 3-kinase
  • the one or more additional therapeutic agent may be an inhibitors of lysyl oxidase-like 2 (LOXL2) or a substance that binds to LOXL2, including for example, a humanized monoclonal antibody (mAb) with an immunoglobulin IgG4 isotype directed against human LOXL2.
  • the one or more additional therapeutic agent may be an inhibitor of apoptosis signal-regulating kinase (ASK-1) or a substance that binds to ASK-1.
  • the one or more additional therapeutic agent may be an inhibitor of a Janus kinase, such as JAK1 or JAK2, or a substance that binds to a Janus kinase, such as JAK1 or JAK2.
  • the one or more additional therapeutic agent is momelotinib.
  • the one or more additional therapeutic agent may be a Bruton's tyrosine kinase (BTK) inhibitor.
  • the one or more additional therapeutic agent may be a B-cell lymphoma (BCL) inhibitor.
  • the BCL inhibitor is a BCL-2 inhibitor.
  • the BCL inhibitor is ABT-199.
  • the one or more additional therapeutic agent may be fludarabine, rituximab, obinutuzumab, alkylating agents, alemtuzumab and other chemotherapy treatments such as CHOP (cyclophosphamide, doxorubicin, vincristine, prednisone); R-CHOP (rituximab-CHOP); hyperCVAD (hyperfractionated cyclophosphamide, vincristine, doxorubicin, dexamethasone, methotrexate, cytarabine); R-hyperCVAD (rituximab-hyperCVAD); FCM (fludarabine, cyclophosphamide, mitoxantrone); R-FCM (rituximab, fludarabine, cyclophosphamide, mitoxantrone); bortezomib and rituximab; temsirolimus and rituxima
  • the one or more additional therapeutic agent may be a vinca-alkaloid.
  • the vinca-alkaloid is selected from the group consisting of vincristine, vinblastine, vindesine, vinorelbine, desoxyvincaminol, vincaminol, vinburnine, vincamajine, andieridine, and pharmaceutically acceptable salts thereof.
  • at least one vinca-alkaloid is selected from the group consisting of vincristine, vinblastine, vindesine, vinorelbine, desoxyvincaminol, vincaminol, vinburnine, vincamajine, andieridine and pharmaceutically acceptable salts thereof.
  • the vinca-alkaloid is selected from the group consisting of vincristine, vinblastine, vindesine, and vinorelbine, and pharmaceutically acceptable salts thereof. In other variations, the vinca-alkaloid is selected from the group consisting of vincristine and vinblastine, and pharmaceutically acceptable salts thereof. In one variation, the vinca-alkaloid is vincristine and pharmaceutically acceptable salts thereof. In another variation, the vinca-alkaloid is vinblastine and pharmaceutically acceptable salts thereof.
  • a method for treating cancer in a human in need thereof comprising administering to the human a bis-mesylate salt of a compound of Formula I, or a hydrate thereof; and a vinca-alkaloid, or a pharmaceutically acceptable salt thereof.
  • a method for treating cancer in a human in need thereof comprising administering to the human a hydrate, bis-mesylate salt of a compound of Formula I; and a vinca-alkaloid, or a pharmaceutically acceptable salt thereof.
  • a method of treating cancer in a human in need thereof comprising administering a monohydrate, bis-mesylate salt of a compound of Formula I; and a vinca-alkaloid, or a pharmaceutically acceptable salt thereof.
  • a method of treating cancer in a human in need thereof comprising administering a mesylate salt of Formula IA or IB, or a hydrate thereof; and a vinca-alkaloid, or a pharmaceutically acceptable salt thereof.
  • a method of treating cancer in a human in need thereof comprising administering a hydrate, mesylate salt of Formula IA or IB; and a vinca-alkaloid, or a pharmaceutically acceptable salt thereof.
  • the one or more additional therapies may be any monotherapy or combination therapy suitable for treating leukemia, including, for example, chronic lymphocytic leukemia (CLL), acute lymphocytic leukemia (ALL), and/or acute myeloid leukemia (AML).
  • CLL chronic lymphocytic leukemia
  • ALL acute lymphocytic leukemia
  • AML acute myeloid leukemia
  • the one or more additional therapeutic agent may be an anti-inflammatory agent. Treatment with the one or more additional therapeutic agent may be prior to, concomitant with, or following treatment with the pharmaceutical composition described herein.
  • the pharmaceutical composition described herein is combined with another therapeutic agent in a single dosage form.
  • Suitable antitumor therapeutics that may be used in combination with at least one chemical entity described herein include, but are not limited to, chemotherapeutic agents, for example mitomycin C, carboplatin, taxol, cisplatin, paclitaxel, etoposide, doxorubicin, or a combination comprising at least one of the foregoing chemotherapeutic agents. Radiotherapeutic antitumor agents may also be used, alone or in combination with chemotherapeutic agents.
  • the pharmaceutical composition described herein can be useful as chemosensitizing agents, and, thus, can be useful in combination with other chemotherapeutic drugs, in particular, drugs that induce apoptosis.
  • a method for increasing sensitivity of cancer cells to chemotherapy comprising administering to a subject (e.g., human) undergoing chemotherapy a chemotherapeutic agent together with the pharmaceutical composition described herein in an amount sufficient to increase the sensitivity of cancer cells to the chemotherapeutic agent is also provided herein.
  • chemotherapeutic drugs that can be used in combination with chemical entities described herein include topoisomerase I inhibitors (camptothesin or topotecan), topoisomerase II inhibitors (e.g. daunomycin and etoposide), alkylating agents (e.g. cyclophosphamide, melphalan and BCNU), tubulin directed agents (e.g. taxol and vinblastine), and biological agents (e.g. antibodies such as anti CD20 antibody, IDEC 8, immunotoxins, and cytokines).
  • topoisomerase I inhibitors camptothesin or topotecan
  • topoisomerase II inhibitors e.g. daunomycin and etopo
  • the pharmaceutical composition described herein are used in combination with Rituxan® (Rituximab) or other agents that work by selectively depleting CD20+ B-cells.
  • Anti-inflammatory agents include but are not limited to NSAIDs, non-specific and COX-2 specific cyclooxgenase enzyme inhibitors, gold compounds, corticosteroids, methotrexate, tumor necrosis factor receptor (TNF) receptors antagonists, immunosuppressants and methotrexate.
  • NSAIDs include, but are not limited to ibuprofen, flurbiprofen, naproxen and naproxen sodium, diclofenac, combinations of diclofenac sodium and misoprostol, sulindac, oxaprozin, diflunisal, piroxicam, indomethacin, etodolac, fenoprofen calcium, ketoprofen, sodium nabumetone, sulfasalazine, tolmetin sodium, and hydroxychloroquine.
  • NSAIDs also include COX-2 specific inhibitors (i.e., a compound that inhibits COX-2 with an IC50 that is at least 50-fold lower than the IC50 for COX-1) such as celecoxib, valdecoxib, lumiracoxib, etoricoxib and/or rofecoxib.
  • COX-2 specific inhibitors i.e., a compound that inhibits COX-2 with an IC50 that is at least 50-fold lower than the IC50 for COX-1
  • celecoxib valdecoxib
  • lumiracoxib etoricoxib
  • etoricoxib etoricoxib
  • rofecoxib rofecoxib
  • the anti-inflammatory agent is a salicylate.
  • Salicylates include but are not limited to acetylsalicylic acid or aspirin, sodium salicylate, and choline and magnesium salicylates.
  • the anti-inflammatory agent may also be a corticosteroid.
  • the corticosteroid may be chosen from cortisone, dexamethasone, methylprednisolone, prednisolone, prednisolone sodium phosphate, and prednisone.
  • the anti-inflammatory therapeutic agent is a gold compound such as gold sodium thiomalate or auranofin.
  • the anti-inflammatory agent is a metabolic inhibitor such as a dihydrofolate reductase inhibitor, such as methotrexate or a dihydroorotate dehydrogenase inhibitor, such as leflunomide.
  • At least one anti-inflammatory compound is an anti-C5 monoclonal antibody (such as eculizumab or pexelizumab), a TNF antagonist, such as entanercept, or infliximab, which is an anti-TNF alpha monoclonal antibody are used.
  • an anti-C5 monoclonal antibody such as eculizumab or pexelizumab
  • a TNF antagonist such as entanercept, or infliximab, which is an anti-TNF alpha monoclonal antibody
  • At least one therapeutic agent is an immunosuppressant compound such as methotrexate, leflunomide, cyclosporine, tacrolimus, azathioprine, or mycophenolate mofetil are used.
  • an immunosuppressant compound such as methotrexate, leflunomide, cyclosporine, tacrolimus, azathioprine, or mycophenolate mofetil
  • the additional therapeutic agents include a PI3K inhibitor and a LOXL2 inhibitor.
  • Kits comprising a pharmaceutical composition comprising a mesylate salt (including, for example, a bis-mesylate salt) of the compound of Formula I, or a hydrate thereof, and at least one pharmaceutical carrier, excipient, adjuvant, or vehicle (e.g., at least one pharmaceutically acceptable polymer) are also provided.
  • a pharmaceutical composition comprising a mesylate salt (including, for example, a bis-mesylate salt) of the compound of Formula I, or a hydrate thereof, and at least one pharmaceutical carrier, excipient, adjuvant, or vehicle (e.g., at least one pharmaceutically acceptable polymer) are also provided.
  • kits comprising a pharmaceutical composition, comprising: a bis-mesylate salt of a compound of Formula I, or a hydrate thereof; and a pharmaceutical carrier, excipient, adjuvant, or vehicle.
  • a pharmaceutical composition comprising: a hydrate, bis-mesylate salt of a compound of Formula I, and a pharmaceutical carrier, excipient, adjuvant, or vehicle.
  • a kit comprising a pharmaceutical composition, comprising: a monohydrate, bis-mesylate salt of a compound of Formula I, and a pharmaceutical carrier, excipient, adjuvant, or vehicle.
  • kits comprising a pharmaceutical composition, comprising: a mesylate salt of Formula IA or IB, or a hydrate thereof; and a pharmaceutical carrier, excipient, adjuvant, or vehicle.
  • a kit comprising a pharmaceutical composition, comprising: a hydrate, mesylate salt of Formula IA or IB; and a pharmaceutical carrier, excipient, adjuvant, or vehicle.
  • a kit comprising a pharmaceutical composition, comprising: polymorph Form 3; and a pharmaceutical carrier, excipient, adjuvant, or vehicle.
  • a kit comprising a pharmaceutical composition, comprising: polymorph Form 7; and a pharmaceutical carrier, excipient, adjuvant, or vehicle.
  • the mesylate salt is bis-mesylate salt of Formula IA.
  • the mesylate salt is Form 3 of bis-mesylate salt of Formula IA, Form 7 of bis-mesylate salt of Formula IA, or a mixture thereof.
  • Form 3 and Form 7 are polymorphic forms of a bis-mesylate salt of a compound of Formula I.
  • Form 3 is a polymorph of a monohydrate, bis-mesylate salt of a compound of Formula I.
  • the kit comprises instructions for use in the treatment of cancer or inflammatory conditions.
  • the instructions are directed to use of the pharmaceutical composition for the treatment of cancer, including for example, leukemia or lymphoma.
  • the cancer is acute lymphocytic leukemia (ALL), acute myeloid leukemia (AML), chronic lymphocytic leukemia (CLL), small lymphocytic lymphoma (SLL), myelodysplastic syndrome (MDS), myeloproliferative disease (MPD), chronic myeloid leukemia (CML), multiple myeloma (MM), indolent non-Hodgkin's lymphoma (iNHL), refractory iNHL, non-Hodgkin's lymphoma (NHL), mantle cell lymphoma (MCL), follicular lymphoma (FL), Waldestrom's macroglobulinemia (WM), T-cell lymphoma, B-cell lymphoma,
  • ALL acute lymphocytic le
  • the cancer is T-cell acute lymphoblastic leukemia (T-ALL), or B-cell acute lymphoblastic leukemia (B-ALL).
  • T-ALL T-cell acute lymphoblastic leukemia
  • B-ALL B-cell acute lymphoblastic leukemia
  • the cancer is MCL, DLBCL, iNHL, FL, MZL, LPL, SLL, or WM.
  • the cancer is CLL, MCL, DLBCL, iNHL (including, for example, non-FL iNHL), or FL.
  • the non-Hodgkin lymphoma encompasses the indolent B-cell diseases that include, for example, follicular lymphoma, lymphoplasmacytic lymphoma, Waldenstrom macroglobulinemia, and marginal zone lymphoma, as well as the aggressive lymphomas that include, for example, Burkitt lymphoma, diffuse large B-cell lymphoma (DLBCL) and mantle cell lymphoma (MCL).
  • the cancer is indolent non-Hodgkin's lymphoma (iNHL).
  • the cancer is non-FL iNHL.
  • the instructions are directed to use of the pharmaceutical composition for the treatment of an autoimmune disease.
  • an autoimmune disease include asthma, rheumatoid arthritis, multiple sclerosis, and lupus.
  • kits may comprise: a) about 34% w/w of a mesylate salt (including, for example, a mono-mesylate or bis-mesylate salt) of a compound of Formula I; b) about 15% w/w HPMC; c) about 22% w/w mannitol; d) about 10% w/w crospovidone; and e) about 1% w/w to about 3% w/w poloxamer.
  • a mesylate salt including, for example, a mono-mesylate or bis-mesylate salt
  • a kit may comprise: a) about 34% w/w of a bis-mesylate salt of a compound of Formula I, or a hydrate thereof; b) about 15% w/w HPMC; c) about 22% w/w mannitol; d) about 10% w/w crospovidone; and e) about 1% w/w to about 3% w/w poloxamer.
  • a kit may comprise: a) about 34% w/w of a monohydrate, bis-mesylate salt of a compound of Formula I; b) about 15% w/w HPMC; c) about 22% w/w mannitol; d) about 10% w/w crospovidone; and e) about 1% w/w to about 3% w/w poloxamer.
  • Articles of manufacture comprising a container in which a pharmaceutical composition comprising a mesylate salt (including, for example, a bis-mesylate salt) of a compound of Formula I and at least one pharmaceutically acceptable polymer are contained are provided.
  • the article of manufacture may be a bottle, vial, ampoule, single-use disposable applicator, or the like, containing the pharmaceutical composition provided in the present disclosure.
  • the container may be formed from a variety of materials, such as glass or plastic and in one aspect also contains a label on, or associated with, the container which indicates directions for use in the treatment of cancer or inflammatory conditions.
  • an article of manufacture comprising a bis-mesylate salt of a compound of Formula I, or a hydrate thereof.
  • an article of manufacture comprising a hydrate, bis-mesylate salt of a compound of Formula I.
  • an article of manufacture comprising a monohydrate, bis-mesylate salt of a compound of Formula I.
  • an article of manufacture comprising a mesylate salt of Formula IA or IB, or a hydrate thereof.
  • provided is an article of manufacture comprising a hydrate, mesylate salt of Formula IA or IB.
  • provided is an article of manufacture comprising polymorph Form 3.
  • an article of manufacture comprising polymorph Form 7.
  • the article of manufacture may further comprise a label containing instructions for use of the mesylate salt.
  • Unit dosage forms of the pharmaceutical composition comprising a mesylate salt (including, for example, a mono-mesylate or bis-mesylate salt) of a compound of Formula I and at least one pharmaceutically acceptable polymer are also provided.
  • a mesylate salt including, for example, a mono-mesylate or bis-mesylate salt
  • a unit dosage comprising a bis-mesylate salt of a compound of Formula I, or a hydrate thereof. In certain aspects, provided is a unit dosage comprising a hydrate, bis-mesylate salt of a compound of Formula I. In one aspect, provided is a unit dosage comprising a monohydrate, bis-mesylate salt of a compound of Formula I. In certain aspects, provided is a unit dosage comprising a mesylate salt of Formula IA or IB, or a hydrate thereof. In other aspects, provided is a unit dosage comprising a hydrate, mesylate salt of Formula IA or IB. In one aspect, provided is a unit dosage comprising polymorph Form 3. In one aspect, provided is a unit dosage comprising polymorph Form 7. In any of the foregoing embodiments, the unit dosage is a tablet.
  • the unit dosage form comprises from about 10 mg to about 1800 mg, or about 10 mg to about 1500 mg, or about 10 mg to about 1300 mg, or about 10 mg to about 1000 mg, or about 10 mg to about 800 mg, or about 10 mg to about 600 mg, or about 10 mg to about 300 mg, or about 10 mg to about 200 mg, or about 10 mg to about 100 mg, or about 100 mg to about 800 mg, or about 100 mg to about 600 mg, or about 100 mg to about 300 mg, or about 100 mg to about 200 mg, or about 200 mg to about 350 mg, or about 250 mg to about 300 mg, or about 200 mg to about 400 mg, or about 400 mg to about 600 mg, or about 400 mg to about 800 mg, or about 600 mg or about 800 mg, or about 800 mg to about 1200 mg, or about 1200 mg to about 1600 of a mesylate salt of a compound of Formula I, or a hydrate thereof; or in certain embodiments, of a mono-mesylate or bis-mesylate salt of a compound of Formula I, a hydrate thereof; or
  • the unit dosage form comprises about 25 mg, about 50 mg, about 75 mg, about 100 mg, about 200 mg, about 250 mg, about 300 mg, about 350 mg, about 400 mg, about 450 mg, about 500 mg, about 550 mg, about 600 mg, about 700 mg, about 800 mg, about 900 mg, about 1000 mg, about 1100 mg, about 1200 mg, or about 1300 mg of a mesylate salt of a compound of Formula I, or a hydrate thereof; or in certain embodiments, of a mono-mesylate or bis-mesylate salt of a compound of Formula I, a hydrate thereof; or in one embodiment, of a monohydrate, bis-mesylate salt of a compound of Formula I; or in another embodiment, polymorph Form 3, polymorph Form 7, or a combination thereof.
  • the unit dosage form further comprises at least one pharmaceutically acceptable carrier.
  • the dosages for oral administration described above may be administered once daily (QD) or twice daily (BID).
  • a mesylate salt of a compound of Formula I, or a hydrate thereof; or in certain embodiments, a mono-mesylate or bis-mesylate salt of a compound of Formula I, a hydrate thereof; or in one embodiment, a monohydrate, bis-mesylate salt of a compound of Formula I; or in another embodiment, polymorph Form 3, polymorph Form 7, or a combination thereof, or a pharmaceutical composition of any of the foregoing is administered orally at a unit dosage of about 1 mg QD, about 2 mg QD, about 5 mg QD, about 10 mg QD, about 15 mg QD, about 20 mg QD, about 25 mg QD, about 30 mg QD, about 35 mg QD, about 40 mg QD, about 45 mg QD, about 50 mg QD, about 75 mg QD, about 100 mg QD, about 125 mg QD, about 150 mg QD, about 175 mg QD,
  • a tablet comprising a mesylate salt of a compound of Formula I, or a hydrate thereof; or in certain embodiments, a mono-mesylate or bis-mesylate salt of a compound of Formula I, a hydrate thereof; or in one embodiment, a monohydrate, bis-mesylate salt of a compound of Formula I; or in another embodiment, polymorph Form 3, polymorph Form 7, or a combination thereof, wherein the tablet is administered orally to a human in need thereof at a unit dosage of about 1 mg BID, about 2 mg BID, about 5 mg BID, about 10 mg BID, about 15 mg BID, about 20 mg BID, about 25 mg BID, about 30 mg BID, about 35 mg BID, about 40 mg BID, about 45 mg BID, about 50 mg BID, about 75 mg BID, about 100 mg BID, about 125 mg BID, about 150 mg BID, about 175 mg BID, about 200 mg BID, about 225 mg BID, about 250 mg B
  • the human has a condition selected from the group consisting of lymphoplasmacytic lymphoma/Waldestrom's macroglobulinemia (LPL/WM), small lymphocytic lymphoma (SLL), marginal zone lymphoma (MZL), follicular lymphoma (FL), diffuse large B-cell lymphoma (DLBCL), mantle cell lymphoma (MCL), and chronic lymphocytic leukemia (CLL), or any combination thereof.
  • LPL/WM lymphoplasmacytic lymphoma/Waldestrom's macroglobulinemia
  • SLL small lymphocytic lymphoma
  • MZL marginal zone lymphoma
  • FL follicular lymphoma
  • DLBCL diffuse large B-cell lymphoma
  • MCL mantle cell lymphoma
  • CLL chronic lymphocytic leukemia
  • the human is (i) refractory to, and/or (ii) in relapse after treatment with at least one therapy for a non-FL indolent non-Hodgkin's lymphoma.
  • the non-FL indolent non-Hodgkin's lymphoma is lymphoplasmacytic lymphoma/Waldestrom's macroglobulinemia (LPL/WM), small lymphocytic lymphoma (SLL), or marginal zone lymphoma (MZL)).
  • LPL/WM lymphoplasmacytic lymphoma/Waldestrom's macroglobulinemia
  • SLL small lymphocytic lymphoma
  • MZL marginal zone lymphoma
  • the human is (i) refractory to, and/or (ii) in relapse after treatment with at least one therapy for follicular lymphoma (FL).
  • the human is (i) refractory to, and/or (ii) in relapse after treatment with at least one therapy for diffuse large B-cell lymphoma (DLBCL).
  • the human is (i) refractory to, and/or (ii) in relapse after treatment with at least one therapy for mantle cell lymphoma (MCL).
  • MCL mantle cell lymphoma
  • the human is (i) refractory to, and/or (ii) in relapse after treatment with at least one therapy for chronic lymphocytic leukemia (CLL).
  • CLL chronic lymphocytic leukemia
  • an article of manufacture may comprise: a) about 34% w/w of a mesylate salt (including, for example, a mono-mesylate or bis-mesylate salt) of a compound of Formula I; b) about 15% w/w HPMC; c) about 22% w/w mannitol; d) about 10% w/w crospovidone; and e) about 1% w/w to about 3% w/w poloxamer.
  • a mesylate salt including, for example, a mono-mesylate or bis-mesylate salt
  • an article of manufacture may comprise: a) about 34% w/w of a bis-mesylate salt of a compound of Formula I, or a hydrate thereof; b) about 15% w/w HPMC; c) about 22% w/w mannitol; d) about 10% w/w crospovidone; and e) about 1% w/w to about 3% w/w poloxamer.
  • an article of manufacture may comprise: a) about 34% w/w of a monohydrate, bis-mesylate salt of a compound of Formula I; b) about 15% w/w HPMC; c) about 22% w/w mannitol; d) about 10% w/w crospovidone; and e) about 1% w/w to about 3% w/w poloxamer.
  • an article of manufacture may comprise: a) about 34% w/w of polymorph Form 3, polymorph Form 7, or a combination thereof; b) about 15% w/w HPMC; c) about 22% w/w mannitol; d) about 10% w/w crospovidone; and e) about 1% w/w to about 3% w/w poloxamer.
  • the polymorphs described herein may be characterized by various methods known in the art, such as X-ray powder diffraction pattern (XRPD), differential scanning calorimetry (DSC), thermal gravimetric analysis (TGA), dynamic vapor sorption (DVS), single crystal X-ray diffraction, nuclear magnetic resonance (NMR, e.g., 1 H NMR), including, for example, the methods described in Examples 4A, 4B, 4C, 5A, 5B, 6A, 6B and 11.
  • exemplary XRPD patterns are provided in FIGS. 1A and 1B ; exemplary DSC and TGA profiles are provided in FIGS. 3A, 3B and 3C ; exemplary DVS plots are provided in FIGS.
  • FIGS. 2A and 2B representative XRPD patterns are provided in FIGS. 2A and 2B ; exemplary DSC and TGA data are provided in FIGS. 4A, 4B and 4C ; exemplary DVS plots are provided in FIGS. 5B and 5D ; and an exemplary 1 H NMR spectrum is provided in FIG. 14 .
  • X refers to weight equivalents
  • V refers to volume equivalents
  • RH refers to relative humidity
  • polymorph Form 3 is a polymorph of a monohydrate, bis-mesylate salt of a compound of Formula I (and may also be described as a polymorph of a monohydrate of the compound of Formula IA shown in the reaction scheme below).
  • a compound of Formula I (1.0 X) was added to Reactor A.
  • Methanesulfonic acid (0.56 X, 2.40 eq)
  • water (4 X, 4 V)
  • acetone 3.2 X, 4 V
  • the contents of Reactor B were added to Reactor A while maintaining the temperature in Reactor A below 35° C.
  • the contents of Reactor A were transferred to Reactor B.
  • Reactor A was rinsed with water (1 X, 1 V) and acetone (0.8 X, 1 V), and transferred to Reactor B.
  • the temperature of Reactor B was adjusted to 19-25° C. Under high agitation, acetone (11.9 X, 15 V) was added to Reactor B.
  • Reactor B The temperature of Reactor B was adjusted to 0-6° C. and the contents of Reactor B were mixed for 5 h, then filtered, and rinsed with acetone (4.0 X, 5 V) to provide polymorph Form 7.
  • Form 7 was dried under vacuum at 60° C. until constant weight was achieved. Representative patterns of XRPD and DSC of polymorph Form 7 are shown in FIGS. 2A and 4A , respectively.
  • the isolated polymorph Form 7 was added to polymorph Form 3 seeds of a compound of Formula IA (0.01 X, 1 mol %) in Reactor B.
  • Acetone (15.4 X, 19.5 V), and water (0.5 X, 0.5 V) were added to Reactor B and mixed at 19-25° C. until polymorph Form 7 was converted to Form 3.
  • the conversion was monitored by XRPD or DSC.
  • the contents of Reactor B was filtered, rinsed with acetone (2.4 X, 3 V) and dried under vacuum at 60° C. until constant weight was achieved.
  • Representative patterns of XRPD and DSC of polymorph Form 3 of a compound of Formula IA are shown in FIGS. 1A and 3A , respectively.
  • Other representative patterns of XRPD and DSC of polymorph Form 3 of a compound of Formula IA are shown in FIGS. 1B and 3C , respectively.
  • polymorph Form 3 a monohydrate, bis-mesylate salt of a compound of Formula I (which may also be described as a polymorph of a monohydrate of the compound of Formula IA shown in the reaction scheme below).
  • Polymorph Form 7 was obtained as described in Example 1.
  • the isolated polymorph Form 7 was added to polymorph Form 3 seeds of a compound of Formula IA (0.01 X, 1 mol %) in Reactor B.
  • Acetone (15.0 X, 19.0 V), and water (1.0 X, 1.0 V) were added to Reactor B.
  • the contents of the Reactor B were heated to reflux (about 55° C.) until polymorph Form 7 was converted to Form 3.
  • the conversion was monitored by XRPD or DSC.
  • the contents of Reactor B was a slurry and was cooled to 19-25° C., then filtered, rinsed with acetone (2.4 X, 3 V) and dried under vacuum at 60° C. until constant weight is achieved to provide the polymorph Form 3.
  • FIGS. 1A and 3A Representative patterns of XRPD and DSC of polymorph Form 3 of a compound of Formula IA are shown in FIGS. 1A and 3A , respectively.
  • Other representative patterns of XRPD and DSC of polymorph Form 3 of a compound of Formula IA are shown in FIGS. 1B and 3C , respectively.
  • the following is a method for producing polymorph Form 3, a monohydrate, bis-mesylate salt of a compound of Formula I (which may also be described as a polymorph of a monohydrate of the compound of Formula IA shown in the reaction scheme below) from a compound of Formula I (as a the free base).
  • the described method uses a single reactor in the conversion of a compound of Formula I to polymorph Form 3, and does not require isolation of a compound intermediate.
  • a compound of Formula I (1.0X) was added to acetone (14.2X, 18V) in Reactor A and mixed.
  • Water (0.8X, 0.8V) was added to Reactor A, followed by methanesulfonic acid, (0.48 X, 2.05 eq).
  • Acetone (0.9 X, 1.2 V) was pumped through to rinse the lines forward to Reactor A with acetone.
  • the contents of Reactor A were heated to reflux (about 55° C.) for about 2 hours.
  • Polymorph Form 3 seeds (0.015 X, 1 mol %) were added to Reactor A and the contents mixed at reflux to convert a compound of Formula IA to polymorph Form 3. The conversion was monitored by XRPD.
  • FIGS. 1A and 3A Representative patterns of XRPD and DSC of polymorph Form 3 of a compound of Formula IA are shown in FIGS. 1A and 3A , respectively.
  • Other representative patterns of XRPD and DSC of polymorph Form 3 of a compound of Formula IA are shown in FIGS. 1B and 3C , respectively.
  • XRPD patterns were collected with a PANalytical X'Pert PRO MPD diffractometer using an incident beam of Cu radiation produced using an Optix long, fine-focus source.
  • An elliptically graded multilayer mirror was used to focus Cu K ⁇ X-rays through the specimen and onto the detector.
  • a silicon specimen NIST SRM 640d was analyzed to verify the observed position of the Si 111 peak is consistent with the NIST-certified position.
  • a specimen of the sample was sandwiched between 3- ⁇ m-thick films and analyzed in transmission geometry.
  • a beam-stop, short antiscatter extension, and an antiscatter knife edge were used to minimize the background generated by air.
  • Soller slits for the incident and diffracted beams were used to minimize broadening from axial divergence.
  • Diffraction patterns were collected using a scanning position-sensitive detector (X'Celerator) located 240 mm from the specimen and Data Collector software v. 2.2b.
  • the data acquisition parameters for each pattern are displayed above the image in the Data section of this report including the divergence slit (DS) before the mirror.
  • Prominent peaks are selected from observed peaks by identifying preferably non-overlapping, low-angle peaks, with strong intensity.
  • FIG. 1A An exemplary XRPD pattern of polymorph Form 3 is shown in FIG. 1A .
  • characteristic XRPD 2 ⁇ -reflections ( ⁇ 0.2 degrees) for Form 3 are 13.75, 16.90, 22.88, and 26.06.
  • XRPD patterns were collected using a PANalytical X'Pert MPD Pro Powder X-Ray Diffractometer configured with reflectance stage with spinning, data acquisition range: 2-40 degrees 2 ⁇ , Copper (Cu) anode; K ⁇ 1/K ⁇ 2 radiation; tube current 40 mA; tube tension 45 kV; automatic divergence and anti-scatter slits. Samples were prepared for analysis by distributing solid material as a thin layer on a silicon holder. Each holder was mounted on a reflectance/transmittance stage and rotated during data acquisition.
  • FIG. 2A An exemplary XRPD pattern of polymorph Form 7 is shown in FIG. 2A .
  • characteristic XRPD 2 ⁇ -reflections ( ⁇ 0.2 degrees) for Form 7 are 4.94, 9.82, and 26.68.
  • This example describes experimental conditions and data for XRPD measurements of polymorph Form 3 and polymorph Form 7.
  • X-Ray Powder Diffraction (XRPD) patterns were collected on a Bruker AXS C2 GADDS diffractometer using Cu K ⁇ radiation (40 kV, 40 mA), automated XYZ stage, laser video microscope for auto-sample positioning and a HiStar 2-dimensional area detector.
  • X-ray optics consists of a single Göbel multilayer mirror coupled with a pinhole collimator of 0.3 mm A weekly performance check is carried out using a certified standard NIST 1976 Corundum (flat plate).
  • the beam divergence i.e. the effective size of the X-ray beam on the sample, was approximately 4 mm
  • a ⁇ - ⁇ continuous scan mode was employed with a sample-detector distance of 20 cm which gives an effective 2 ⁇ range of 3.2°-29.7°.
  • the sample would be exposed to the X-ray beam for 120 seconds.
  • the software used for data collection was GADDS for XP/2000 4.1.43 and the data were analysed and presented using Diffrac Plus EVA v15.0.0.0.
  • Ambient conditions Samples run under ambient conditions were prepared as flat plate specimens using powder as received without grinding. Approximately 1-2 mg of the sample was lightly pressed on a glass slide to obtain a flat surface.
  • Non-ambient conditions Samples run under non-ambient conditions were mounted on a silicon wafer with heat-conducting compound. The sample was then heated to the appropriate temperature at 20° C./min and subsequently held isothermally for 1 minute before data collection was initiated.
  • This example describes experimental conditions and data for XRPD measurements of polymorph Form 3 and polymorph Form 7.
  • X-Ray Powder Diffraction (XRPD) patterns were collected on a Bruker D8 diffractometer using Cu K ⁇ radiation (40 kV, 40 mA), ⁇ -2 ⁇ goniometer, and divergence of V4 and receiving slits, a Ge monochromator and a Lynxeye detector.
  • the instrument is performance checked using a certified Corundum standard (NIST 1976).
  • the software used for data collection was Diffrac Plus XRD Commander v2.6.1 and the data were analysed and presented using Diffrac Plus EVA v15.0.0.0.
  • Samples run under non-ambient conditions Approximately 40 mg of the sample was placed in a Ni-coated sample holder under ambient conditions. The sample was loaded at 25° C. The sample was then heated to the appropriate temperature. The details of the data collection are:
  • This example describes the experimental conditions and data for Modulated Differential Scanning calorimetry (mDSC) and Thermal Gravimetric Analysis (TGA) measurements of polymorph Form 3 and polymorph Form 7.
  • mDSC Modulated Differential Scanning calorimetry
  • TGA Thermal Gravimetric Analysis
  • DSC was performed with approximately 2 to 5 mg of solid in an aluminum pan with a pinhole, heated at 2° C./min with a modulation of ⁇ 0.8° C. every 60 seconds under dried nitrogen purge using TA instruments (New Castle, Del., USA) model 1000.
  • TGA was performed with approximately 2 to 5 mg of solid heated at a rate of 2 or 10° C./min using TA Instruments (New Castle, Del., USA) model 500.
  • the DSC thermogram and the overlay of the DSC and TGA thermograms of polymorph Form 3 are shown in FIGS. 3A and 3B , respectively.
  • the characteristic DSC pattern of Form 3 was observed to have three endotherms that onset at 109° C., 206° C., and 255° C.
  • the major event in the DSC thermogram observed was melting at 260° C. with concurrent decomposition.
  • a minor endotherm was observed at ⁇ 170° C. and a minor exotherm at ⁇ 210° C. the origins of which were unknown.
  • the TGA thermogram of Form 3 in this example shows a step change of ⁇ 3% between the temperature of 75° C. and 100° C. which could be due to loss of residual solvent.
  • Another step change around 200° C. was observed, which coincides with the small exotherm on the DSC thermogram.
  • the major decomposition was observed to start around 230° C.
  • the DSC thermogram and the overlay of the DSC and TGA thermograms of Form 7 are shown in FIGS. 4A and 4B , respectively.
  • Two small endotherms were observed at ⁇ 110° C. and 170° C. prior to the onset of decomposition at ⁇ 280° C.
  • the reversing heat flow show a typical tg at 170° C. which may indicate that the endotherm shown on the total heat flow at the same temperature is not a melting event. These thermal properties may be consistent with a liquid crystal state.
  • the TGA thermogram of Form 7 shows two step changes of ⁇ 1% each below 80° C. which could be due to loss of residual solvent.
  • the onsite of decomposition was observed at ⁇ 220° C. which is similar to the DSC thermogram.
  • This example describes the experimental conditions and data for Differential Scanning calorimetry (DSC) and Thermal Gravimetric Analysis (TGA) measurements of polymorphs Form 3 and Form 7.
  • DSC Differential Scanning calorimetry
  • TGA Thermal Gravimetric Analysis
  • DSC data were collected on a Mettler DSC 823E equipped with a 34 position auto-sampler.
  • the instrument was calibrated for energy and temperature using certified indium. Typically 0.5-3 mg of each sample, in a pin-holed aluminium pan, was heated at 10° C./min from 25° C. to 300° C. A nitrogen purge at 50 ml/min was maintained over the sample.
  • the instrument control and data analysis software was STARe v12.1. TGA data were collected on a Mettler TGA/SDTA 851e equipped with a 34 position auto-sampler. The instrument was temperature calibrated using certified indium.
  • each sample was loaded onto a pre-weighed aluminium crucible and was heated at 10° C./min from ambient temperature to 350° C. A nitrogen purge at 50 ml/min was maintained over the sample.
  • the instrument control and data analysis software was STARe v12.1.
  • Exemplary DSC and TGA thermograms of polymorph Form 3 and polymorph Form 7 are shown in FIGS. 3C and 4C , respectively.
  • Hygroscopicity of polymorph Form 3 and polymorph Form 7 at 25° C. was determined by dynamic vapor sorption (DVS) using an automated vapor sorption balance (Q5000SA; TA instruments, New Castle, Del.). Hygroscopicity was studied as a function of relative humidity (RH) over the range of 0 to 90%. Approximately 10 mg of solid sample was placed directly onto the 13 mm quartz DVS sample cup and equilibrated at 0% relative humidity (RH) until a constant weight was achieved. After equilibration, the RH was increased in increments of 10% to reach 90%, and the water sorption was monitored. For desorption, the relative humidity was deceased in a similar manner to accomplish a full sorption/desorption cycle.
  • the instrument was operated in dm/dt mode (mass variation over time) to determine when equilibrium was reached.
  • a fixed dm/dt value of 0.0010% min ⁇ 1 was selected for all RH segments.
  • a maximum stage of 360 minutes and a minimum stage of 60 minutes were selected for these experiments.
  • Polymorph Form 3 was not observed to be hygroscopic at 70% RH and below. Above 70% RH, it was observed to absorb a large amount of moisture. At 90%, the water adsorption was observed to reach ⁇ 28%. The water adsorption was observed to be irreversible as indicated by the existence of a big hysteresis. At the end of the DVS experiment, sample was collected and analyzed by XRPD which showed the sample had converted to Form 7. See FIG. 6 .
  • Polymorph Form 7 was observed to be highly hygroscopic. It was observed to continuously adsorb water at all tested RH's. At 40% RH, the weight gain was observed to be nearly 10%. However, the weight gain at 90% RH was observed to be ⁇ 22% which is lower than that of Form 3. As RH decreases, the sample loses weight and the weight gain were observed to return to near zero at 0% RH.
  • polymorph Form 3 was not observed to be hygroscopic and exhibits increased stability compared to Form 7 which adsorbs water below 70% RH.
  • Sorption isotherms were obtained using a Hiden IGASorp moisture sorption analyser, controlled by CFRSorp software.
  • the sample temperature was maintained at 25° C. by a Huber re-circulating water bath.
  • the humidity was controlled by mixing streams of dry and wet nitrogen, with a total flow rate of 250 ml/min.
  • the relative humidity (RH) was measured by a calibrated Vaisala RH probe (dynamic range of 0-95% RH), located near the sample.
  • the weight change, (mass relaxation) of the sample as a function of % RH was constantly monitored by the microbalance (accuracy ⁇ 0.001 mg).
  • a moisture sorption isotherm was performed as outlined in Table 1 below (2 scans giving 1 complete cycle). The standard isotherm was performed at 25° C. at 10% RH intervals over a 0-90% RH range.
  • the software uses a least squares minimization procedure together with a model of the mass relaxation, to predict an asymptotic value.
  • the measured mass relaxation value must be within 5% of that predicted by the software, before the next % RH value is selected.
  • the minimum equilibration time was set to 1 hour and the maximum to 4 hours.
  • Data analysis was carried out using IGASorp Systems Software v 6.50.55. The sample was recovered after completion of the isotherm and re-analyzed by XRPD.
  • sorption isotherms were also obtained using a SMS DVS Intrinsic moisture sorption analyser, controlled by DVS Intrinsic Control software v1.0.1.2 (or v 1.0.1.3).
  • the sample temperature was maintained at 25° C. by the instrument controls.
  • the humidity was controlled by mixing streams of dry and wet nitrogen, with a total flow rate of 200 ml/min.
  • the relative humidity was measured by a calibrated Rotronic probe (dynamic range of 1.0-100% RH), located near the sample.
  • the weight change, (mass relaxation) of the sample as a function of % RH was constantly monitored by the microbalance (accuracy ⁇ 0.005 mg).
  • Polymorph Form 3 was not observed to be hygroscopic at 70% RH and below ( FIG. 5C ). However, Form 3 absorbs up to ⁇ 20 wt. % of water up to 90% RH at 25° C. XRPD analysis of the sample after DVS analysis shows that the material had converted to Form 7.
  • Polymorph Form 7 was observed to be highly hygroscopic ( FIG. 5D ) picking up ⁇ 21 wt. % of water up to 90% RH at 25° C. The form was found to be stable at room temperature between 30-60% RH. However, below this interval the material becomes amorphous. In summary, up to 70% RH, polymorph Form 3 is not hygroscopic and exhibits better stability compared to Form 7.
  • NMR spectra were collected on a Bruker 400 MHz instrument equipped with an auto-sampler and controlled by a DRX400 console. Automated experiments were acquired using ICON-NMR v4.0.7 running with Topspin v1.3 using the standard Bruker loaded experiments. For non-routine spectroscopy, data were acquired through the use of Topspin alone.
  • FIGS. 13 and 14 are exemplary proton NMR spectra of polymorph Form 3 and polymorph Form 7, respectively.
  • polymorph Form 3 i.e., a polymorph of a monohydrate, bis-mesylate salt of a compound of Formula I
  • polymorph Form 3 i.e., a polymorph of a monohydrate, bis-mesylate salt of a compound of Formula I
  • Intrinsic dissolution rate experiments were conducted using a rotating disk apparatus.
  • Disks of drug substance were prepared by directly compressing 100 mg of powder of polymorph Form 3 in a die at a pressure of 2500 psi for 2 minutes using a hydraulic press (Carver Press, Fred Carver, NJ, USA). The exposed surface area for the resulting disk was 0.5 cm 2 .
  • a regular USP dissolution apparatus maintained at 37 ⁇ 0.5° C. was used for the dissolution study.
  • Each dissolution vessel contained 500 mL of aqueous dissolution medium of 0.05 N HCl or pH 6.8 20 mM phosphate buffer with 1% cetyltrimetrylammonium bromide (CTAB).
  • CTAB cetyltrimetrylammonium bromide
  • the disk holder (die) was half-immersed into the dissolution medium and rotated at 100 rpm. Samples were withdrawn at specified time intervals and analyzed by HPLC.
  • the dissolution rate (J) of solid per unit surface area may be described by a diffusion model:
  • Equation 2 Equation 2 is reduced to:
  • IDR intrinsic dissolution rates
  • the IDR of the crystal form of the mono-MSA compound of Formula I showed high pH dependency. From pH 1.3 to 6.8, IDR decreased by about 1000 fold, from 2.5 to 0.003 mg/min/cm 2 . In contrast, The IDR of the crystal form of polymorph Form 3 showed much higher IDR than the mono-MSA at pH 6.8. At 1.2 mg/min/cm 2 , the IDR of polymorph Form 3 is 400-fold higher than mono MSA. In addition, FIG. 7 shows a graphical comparison of the dissolution rate of the two compounds at pH 6.8.
  • Polymorph Form 3 has the unexpected and surprising result of performing significantly better than the mono-MSA compound in increased pH conditions.
  • This example demonstrates the pharmacokinetic effects of administering the mono-MSA salt or the bis-MSA salt (polymorph Form 3, which is a polymorph of a monohydrate, bis-MSA salt) of Formula I to pentagastrin- or famotidine-pretreated dogs.
  • Pentagastrin pretreatment (6 ⁇ g/kg intramuscular injection 30 minutes prior to test formulation dosing) in fasted dogs was used to simulate fasting gastric pH in humans.
  • Famotidine pretreatment (20 mg orally 1 hour prior to test formulation dosing) in fasted dogs was used to simulate human gastric pH when acid suppressant was co-dosed with test formulation.
  • the tablets of the mono-MSA salt or bis-MSA salt of a compound of Formula I (conventional tablet and spray dried formulation) were orally administered followed by a 30 mL water flush.
  • Polymorph Form 3 is a polymorph of a monohydrate, bis-mesylate salt of a compound of Formula I (and may also be described is a polymorph of a monohydrate of the compound of Formula IA shown in the reaction scheme below).
  • Formic acid (3V, 3.6X) and ethyl acetate (2V, 1.8X) were added to Reactor A and adjusted to 19-25° C.
  • the compound of Formula I (1.0X) was added to Reactor A while maintaining the reactor temperature at 19-25° C., and mixed until the solids dissolved.
  • the solution in Reactor A was transferred to Reactor B.
  • Formic acid (0.08V, 0.1X), ethyl acetate (2V, 1.8X), and methanesulfonic acid (2.0 mol equiv., 0.47X) were added to Reactor A.
  • the solution in Reactor A was transferred to Reactor B while maintaining a temperature of 19-25° C. in Reactor B.
  • Ethyl acetate (5V, 4.5X) was added to Reactor A and then to Reactor B over a minimum of 1 hour. The contents of Reactor B were agitated for about 16 h at 19-25° C., then filtered rinsed with ethyl acetate (4V, 3.6X) and dried under vacuum at 60° C.
  • the solids were transferred to Reactor A along with acetone (18.9V, 15.0X) and water (0.5V, 0.5X).
  • the pump and lines were rinsed forward to Reactor A with acetone (0.9V, 0.7X).
  • the contents of Reactor A were mixed at 19-25° C.
  • the conversion was monitored by XRPD analysis.
  • the reaction mixture was filtered, rinsed with acetone (3V, 2.4X) and dried under vacuum at 60° C. to obtain polymorph Form 3.
  • polymorph Form 3 is a polymorph of a monohydrate, bis-mesylate salt of a compound of Formula I as depicted in the reaction scheme below.
  • a mixture of methanesulfonic acid (0.56 X) and water (0.5 X) were added to a mixture of compound of formula I (1.0 X), acetone (3.6 X) and water (4.0 X) while maintaining a content temperature of below about 35° C. until solids dissolved.
  • the solution was agitated for about 10 minutes and the content temperature was adjusted to about 19 to 25° C.
  • acetone (11.9 X) was added over about 2 h. After this period, the content temperature was adjusted to about 0 to 6° C.
  • the mixture was agitated for at least about 5 h, filtered and the filter cake was rinsed with acetone (4.0 X).
  • the wet cake was dried under vacuum at a maximum of about 60° C.
  • This example describes the single crystal X-ray structure of polymorph Form 3 of a monohydrate, bis-mesylate salt of a compound of Formula I.
  • a crystal of sufficient size and quality for analysis by single crystal X-ray diffraction was obtained by maturation between 50° C. and RT on an eight hour cycle in isopropyl acetate.
  • the crystal was of block morphology with approximate dimensions 0.50 ⁇ 0.22 ⁇ 0.15 mm.
  • polymorph Form 3 The structure of polymorph Form 3 was been determined as depicted in FIGS. 10A, 10B, 11 and 12 .
  • Table 5 summarizes the sample and crystal data for polymorph Form 3.
  • polymorph Form 3 which is a polymorph of a monohydrate, bis-MSA salt of a compound of Formula I
  • mono-MSA salt of a compound of Formula I This example provides a description of the synthesis of polymorph Form 3 (which is a polymorph of a monohydrate, bis-MSA salt of a compound of Formula I) from a mono-MSA salt of a compound of Formula I.
  • Formic acid (3.7 X) is charged to a reaction vessel and combined with ethyl acetate (9.7 X). The contents are agitated and adjusted to about 19 to 25° C.
  • the mono-MSA compound of Formula I (1.0 X) is charged portion-wise while maintaining the content temperature at about 19 to 25° C. The contents are agitated at about 19 to 25° C. until all solids dissolve.

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MX2016001304A (es) 2016-04-07
EP3027618B1 (en) 2020-07-08
SG11201600385TA (en) 2016-02-26
HUE052090T2 (hu) 2021-04-28
JP2016527268A (ja) 2016-09-08
AP2016009008A0 (en) 2016-01-31
US10266539B2 (en) 2019-04-23
AU2014296308C1 (en) 2018-09-13

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