Classifications

• • C—CHEMISTRY; METALLURGY
  • C07—ORGANIC CHEMISTRY
  • C07D—HETEROCYCLIC COMPOUNDS
  • C07D413/00—Heterocyclic compounds containing two or more hetero rings, at least one ring having nitrogen and oxygen atoms as the only ring hetero atoms
  • C07D413/02—Heterocyclic compounds containing two or more hetero rings, at least one ring having nitrogen and oxygen atoms as the only ring hetero atoms containing two hetero rings
  • C07D413/12—Heterocyclic compounds containing two or more hetero rings, at least one ring having nitrogen and oxygen atoms as the only ring hetero atoms containing two hetero rings linked by a chain containing hetero atoms as chain links
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
  • A61K31/00—Medicinal preparations containing organic active ingredients
  • A61K31/33—Heterocyclic compounds
  • A61K31/395—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins
  • A61K31/41—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having five-membered rings with two or more ring hetero atoms, at least one of which being nitrogen, e.g. tetrazole
  • A61K31/42—Oxazoles
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
  • A61K31/00—Medicinal preparations containing organic active ingredients
  • A61K31/33—Heterocyclic compounds
  • A61K31/395—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins
  • A61K31/435—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having six-membered rings with one nitrogen as the only ring hetero atom
  • A61K31/44—Non condensed pyridines; Hydrogenated derivatives thereof
  • A61K31/4427—Non condensed pyridines; Hydrogenated derivatives thereof containing further heterocyclic ring systems
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
  • A61P25/00—Drugs for disorders of the nervous system
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
  • A61P25/00—Drugs for disorders of the nervous system
  • A61P25/28—Drugs for disorders of the nervous system for treating neurodegenerative disorders of the central nervous system, e.g. nootropic agents, cognition enhancers, drugs for treating Alzheimer's disease or other forms of dementia
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
  • A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
• • C—CHEMISTRY; METALLURGY
  • C07—ORGANIC CHEMISTRY
  • C07D—HETEROCYCLIC COMPOUNDS
  • C07D413/00—Heterocyclic compounds containing two or more hetero rings, at least one ring having nitrogen and oxygen atoms as the only ring hetero atoms
  • C07D413/14—Heterocyclic compounds containing two or more hetero rings, at least one ring having nitrogen and oxygen atoms as the only ring hetero atoms containing three or more hetero rings
• • C—CHEMISTRY; METALLURGY
  • C07—ORGANIC CHEMISTRY
  • C07D—HETEROCYCLIC COMPOUNDS
  • C07D417/00—Heterocyclic compounds containing two or more hetero rings, at least one ring having nitrogen and sulfur atoms as the only ring hetero atoms, not provided for by group C07D415/00
  • C07D417/14—Heterocyclic compounds containing two or more hetero rings, at least one ring having nitrogen and sulfur atoms as the only ring hetero atoms, not provided for by group C07D415/00 containing three or more hetero rings
• • C—CHEMISTRY; METALLURGY
  • C07—ORGANIC CHEMISTRY
  • C07D—HETEROCYCLIC COMPOUNDS
  • C07D487/00—Heterocyclic compounds containing nitrogen atoms as the only ring hetero atoms in the condensed system, not provided for by groups C07D451/00 - C07D477/00
  • C07D487/02—Heterocyclic compounds containing nitrogen atoms as the only ring hetero atoms in the condensed system, not provided for by groups C07D451/00 - C07D477/00 in which the condensed system contains two hetero rings
  • C07D487/04—Ortho-condensed systems
• • C—CHEMISTRY; METALLURGY
  • C07—ORGANIC CHEMISTRY
  • C07D—HETEROCYCLIC COMPOUNDS
  • C07D491/00—Heterocyclic compounds containing in the condensed ring system both one or more rings having oxygen atoms as the only ring hetero atoms and one or more rings having nitrogen atoms as the only ring hetero atoms, not provided for by groups C07D451/00 - C07D459/00, C07D463/00, C07D477/00 or C07D489/00
  • C07D491/02—Heterocyclic compounds containing in the condensed ring system both one or more rings having oxygen atoms as the only ring hetero atoms and one or more rings having nitrogen atoms as the only ring hetero atoms, not provided for by groups C07D451/00 - C07D459/00, C07D463/00, C07D477/00 or C07D489/00 in which the condensed system contains two hetero rings
  • C07D491/08—Bridged systems
• • C—CHEMISTRY; METALLURGY
  • C07—ORGANIC CHEMISTRY
  • C07D—HETEROCYCLIC COMPOUNDS
  • C07D491/00—Heterocyclic compounds containing in the condensed ring system both one or more rings having oxygen atoms as the only ring hetero atoms and one or more rings having nitrogen atoms as the only ring hetero atoms, not provided for by groups C07D451/00 - C07D459/00, C07D463/00, C07D477/00 or C07D489/00
  • C07D491/02—Heterocyclic compounds containing in the condensed ring system both one or more rings having oxygen atoms as the only ring hetero atoms and one or more rings having nitrogen atoms as the only ring hetero atoms, not provided for by groups C07D451/00 - C07D459/00, C07D463/00, C07D477/00 or C07D489/00 in which the condensed system contains two hetero rings
  • C07D491/10—Spiro-condensed systems

Definitions

• GABA gamma-aminobutyric acid
• GABA A receptors which are members of the ligand-gated ion channel superfamily
• GABA B receptors which are members of the G-protein linked receptor family.
• the GABA A receptor complex which is a membrane-bound heteropentameric protein polymer is composed principally of ⁇ , ⁇ and ⁇ subunits.
• ⁇ 1 ⁇ 2 ⁇ 2 mimics many effects of the classical type-I BzR subtypes, whereas ⁇ 2 ⁇ 2 ⁇ 2, ⁇ 3 ⁇ 2 ⁇ 2 and ⁇ 5 ⁇ 2 ⁇ 2 ion channels are termed type-II BzR.
• ⁇ -CCM benzodiazepine receptor inverse agonist
• ⁇ -CCM and other conventional benzodiazepine receptor inverse agonists are proconvulsant or convulsant which prevents their use as cognition enhancing agents in humans.
• GABA A ⁇ 5 receptor partial or full inverse agonist which is relatively free of activity at GABA A ⁇ 1 and/or ⁇ 2 and/or ⁇ 3 receptor binding sites can be used to provide a medicament which is useful for enhancing cognition with reduced or without proconvulsant activity.
• GABA A ⁇ 5 inverse agonists which are not free of activity at GABA A ⁇ 1 and/or ⁇ 2 and/or ⁇ 3 receptor binding sites but which are functionally selective for ⁇ 5 containing subunits.
• inverse agonists which are selective for GABA A ⁇ 5 subunits and are relatively free of activity at GABA A ⁇ 1, ⁇ 2 and ⁇ 3 receptor binding sites are preferred.
• the present invention provides isoxazole-pyridine derivatives having affinity and selectivity for GABA A ⁇ 5 receptor binding site, their manufacture, pharmaceutical compositions containing them and their use as cognitive enhancer or for the treatment of cognitive disorders like Alzheimer's disease.
• the present invention provides isoxazole-pyridine derivatives of formula I
• the most preferred indication in accordance with the present invention is Alzheimer's disease.
• alkyl denotes a saturated straight- or branched-chain hydrocarbon group containing from 1 to 7 carbon atoms, for example, methyl, ethyl, propyl, isopropyl, n-butyl, iso-butyl, sec-butyl, tert-butyl and the like.
• Preferred alkyl groups are groups with 1 to 4 carbon atoms.
• halo or “halogen” denotes chloro, iodo, fluoro and bromo.
• halo-C 1-7 -alkyl denotes a C 1-7 -alkyl group as defined above wherein at least one of the hydrogen atoms of the alkyl group is replaced by a halogen atom, preferably fluoro or chloro, most preferably fluoro.
• halo-C 1-7 -alkyl examples include but are not limited to methyl, ethyl, propyl, isopropyl, isobutyl, sec-butyl, tert-butyl, pentyl or n-hexyl substituted by one or more Cl, F, Br or I atom(s), in particular one, two or three fluoro or chloro, as well as those groups specifically illustrated by the examples herein below.
• the preferred halo-C 1-7 -alkyl groups are difluoro- or trifluoro-methyl or -ethyl.
• hydroxy-C 1-7 -alkyl denotes a C 1-7 -alkyl group as defined above wherein at least one of the hydrogen atoms of the alkyl group is replaced by a hydroxy group.
• hydroxy-C 1-7 -alkyl examples include but are not limited to methyl, ethyl, propyl, isopropyl, isobutyl, sec-butyl, tert-butyl, pentyl or n-hexyl substituted by one or more hydroxy group(s), in particular with one, two or three hydroxy groups, preferably with one hydroxy group, as well as those groups specifically illustrated by the examples herein below.
• cyano-C 1-7 -alkyl denotes a C 1-7 -alkyl group as defined above wherein at least one of the hydrogen atoms of the alkyl group is replaced by a cyano group.
• hydroxy-C 1-7 -alkyl examples include but are not limited to methyl, ethyl, propyl, isopropyl, isobutyl, sec-butyl, tert-butyl, pentyl or n-hexyl substituted by one or more cyano group(s), preferably by one, two or three, and more preferably by one cyano group, as well as those groups specifically illustrated by the examples herein below.
• alkoxy denotes a group —O-R wherein R is alkyl as defined above.
• aryl refers to a monovalent aromatic carbocyclic ring system, preferably to phenyl or naphthyl, and more preferably to phenyl.
• Aryl is optionally substituted as described herein. If not further indicated, phenyl may optionally be substituted with one or more, in particular with 1, 2, or 3, and more preferably with 1 or 2 substituents selected from halo, CN, NO 2 , hydroxy, C 1-7 alkyl, C 1-7 alkoxy, C 1-7 haloalkyl, C 1-7 hydroxyalkyl, C 1-7 cyanoalkyl, C 1-7 and C 3-7 cycloalkyl.
• aromatic means aromatic according to Hückel's rule.
• a cyclic molecule follows
• halo-C 1-7 -alkoxy examples include but are not limited to methyl, ethyl, propyl, isopropyl, isobutyl, sec-butyl, tert-butyl, pentyl or n-hexyl substituted by one or more Cl, F, Br or I atom(s), in particular one, two or three fluoro or chloro atoms, as well as those groups specifically illustrated by the examples herein below.
• the preferred halo-C 1-7 -alkoxy groups are difluoro- or trifluoro-methoxy or -ethoxy substituted as described above, preferably —OCF 3 .
• cycloalkyl refers to a monovalent saturated cyclic hydrocarbon radical of 3 to 7 ring carbon atoms, preferably 3 to 6 carbon atoms, such as cyclopropyl, cyclobutyl, cyclopentyl, or cyclohexyl.
• heterocycloalkyl refers to a monovalent 3 to 7 membered saturated monocyclic ring containing one, two or three ring heteroatoms selected from N, O and S. One or two ring heteroatoms are preferred. Preferred are 4 to 6 membered heterocycloalkyl or 5 to 6 membered heterocycloalkyl, each containing one or two ring heteroatoms selected from N, O and S. Examples for heterocycloalkyl moieties are tetrahydrofuranyl, tetrahydropyranyl, pyrrolidinyl, morpholinyl, thiomorpholinyl, piperidinyl, or piperazinyl. “Heterocycloalkyl” is hence a subgroup of “heterocyclyl” as defined below. Heterocycloalkyl is optionally substituted as described herein.
• heteroaryl refers to a monovalent aromatic 5- or 6-membered monocyclic ring containing one, two, or three ring heteroatoms selected from N, O, and S, the remaining ring atoms being C.
• the 5- or 6-membered heteroaryl ring contains one or two ring heteroatoms. 6-membered heteroaryl are preferred.
• heteroaryl moieties include but are not limited to furanyl, thiophenyl, pyridinyl, pyrimidinyl, pyrazinyl, pyrazolyl, imidazolyl, oxazolyl, isoxazolyl, thiazolyl, isothiazolyl, triazolyl, 1,2,4-oxadiazolyl, or 1,3,4-oxadiazolyl.
• Preferred heteroaryl groups are pyridinyl, pyrazolyl, isoxazolyl, thiazolyl, or 1,2,4-oxadiazolyl.
• heterocyclyl or “heterocyclyl moiety” refers to a monovalent saturated or partially saturated 3- to 7-membered monocyclic or 9- to 10-membered bicyclic ring system wherein one, two, three or four ring carbon atoms have been replaced by N, O or S, and with the attachment point on the saturated or partially unsaturated ring of said ring system.
• Such bicyclic heterocyclyl moieties hence include aromatic rings annelated to saturated rings.
• heterocyclyl moiety further includes cases where two residues R′ and R′′ together with the nitrogen to which they are bound form such a heterocyclyl moiety.
• heterocyclyl examples include but are not limited to tetrahydropyridinyl, isochromanyl, chromanyl, oxethanyl, isoxazolidinyl, dihydropyridazinyl, tetrahydrofuranyl, tetrahydropyranyl, tetrahydrothiopyranyl, piperidinyl, piperazinyl, pyrrolidinyl, as well as morpholinyl, thiomorpholinyl, 5,6,7,8-tetrahydro-[1,2,4]triazolo[4,3-a]pyrazinyl, 4,5,6,7-tetrahydro-pyrazolo[1,5-a]pyrimidinyl, hexahydrothiopyranyl, or 6-oxa-3-aza-bicyclo[3.1.1]heptanyl.
• substituted heterocyclyl examples include, but are not limited to oxetan-3-ol, 3-oxoisazolidinyl, 3-oxo-dihydropyridazinyl, 6-methyl-3-oxo-dihydropyridazinyl, 2,2-dimethyl-tetrahydropyranyl, tetrahydrothiopyranyl dioxide, N-methyl-piperidinyl, N-ethyl-piperidinyl, N-isopropyl-piperidinyl, N-benzyl-piperidinyl, piperidin-1-yl-acetic t-butyl ester, piperidin-1-yl-acetic acid ethyl ester, piperidin-1-yl-acetic acid, N-(1-ethylcarbamoylmethyl-piperidinyl), N-(1-cyclopropylcarbamoylmethylpiperidinyl), N- ⁇ 1[(2,2,
• spirocyclic heterocycle denotes a saturated bicyclic ring system wherein the two rings have one carbon atom in common.
• the spirocyclic heterocycle may be from 7- to 12-membered, preferably from 7- to 11-membered.
• 2-oxa-6-aza-spiro[3.3]heptyl may be mentioned.
• the spirocyclic heterocycle may be optionally substituted as described herein.
• oxo when referring to substituents on heterocycloalkyl, heterocyclyl or on a heterocycle means that an oxygen atom is attached to the ring. Thereby, the “oxo” may either replace two hydrogen atoms on a carbon atom, or it may simply be attached to sulfur, so that the sulfur exists in oxidized form, i.e. bearing one or two oxygens.
• one or more means from one substituent to the highest possible number of substitution, i.e. replacement of one hydrogen up to replacement of all hydrogens by substituents. Thereby, one, two or three substituents are preferred.
• “Pharmaceutically acceptable,” such as pharmaceutically acceptable carrier, excipient, etc., means pharmacologically acceptable and substantially non-toxic to the subject to which the particular compound is administered.
• pharmaceutically acceptable salt or “pharmaceutically acceptable acid addition salt” embraces salts with inorganic and organic acids, such as hydrochloric acid, nitric acid, sulfuric acid, phosphoric acid, citric acid, formic acid, fumaric acid, maleic acid, acetic acid, succinic acid, tartaric acid, methane-sulfonic acid, p-toluenesulfonic acid and the like.
• “Therapeutically effective amount” means an amount that is effective to prevent, alleviate or ameliorate symptoms of disease or prolong the survival of the subject being treated.
• X is O or NH.
• R 1 is phenyl, pyridinyl, or pyrimidinyl, each optionally substituted with one, two or three halo.
• Preferred halo substituents are chloro and fluoro.
• phenyl is optionally substituted with one, two or three, more preferably with one or two halo substituents selected from chloro and fluoro.
• the halo substituents are located at the ortho, meta or para-position or at the meta and para position of the phenyl ring in respect to the attachment to the isoxazole.
• R 2 is methyl or trifluoromethyl.
• R 3 , R 4 , R 5 , and R 6 are as defined above.
• R 3 is H, halo, CN or C 1-7 alkyl.
• R 3 is H, CN or C 1-4 alkyl. More preferably, R 3 is H, CN or methyl.
• R 6 is H, halo, CN or C 1-7 alkyl.
• R 6 is H, halo or C 1-4 alkyl, more preferably, R 6 is H, Br or C 1-4 alkyl. Even more ore preferably, R 6 is H, Br or methyl.
• R 4 and R 5 are each independently as defined above.
• R 4 and R 5 are each independently as defined above and R 3 and R 6 are each independently H, halo, CN or C 1-7 alkyl.
• R 4 or R 5 are
• R 4 is H
• R 4 is H.
• R 4 is C 1-7 alkyl, optionally substituted with one or more halo, cyano, or hydroxy.
• R 4 is CN
• R 4 is NO 2 .
• R 4 is —C(O)—R a , wherein R a is hydroxy, C 1-7 alkoxy, C 1-7 alkyl, phenoxy or phenyl.
• R 4 is benzyloxy, optionally substituted with one or more E, wherein E is as described as above.
• R 4 is 3- to 7-membered heterocyclyl, optionally substituted with one or more A.
• R 4 in such an embodiment is a 3- to 7-membered heterocycloalkyl, optionally substituted with one or more A.
• A is as described above.
• R 4 is oxethanyl, substituted with one OH.
• R 4 is —C(O)—NR b R c , wherein R b is H or C 1-7 alkyl and R c is
• heteroaryl in this embodiment comprise pyridinyl, pyrazolyl, isoxazolyl, thiazolyl, or 1,2,4-oxadiazolyl, each optionally substituted by one or more E as defined herein.
• heterocyclyl in -(CH 2 ),-heterocyclyl comprise tetrahydropyridinyl, isochromanyl, oxethanyl, isoxazolidinyl, dihydropyridazinyl, tetrahydrofuranyl, tetrahydropyranyl, tetrahydrothiopyranyl, piperidinyl, or pyrrolidinyl, each optionally substituted as described above.
• R 4 is —C(O)—NR b R c , wherein R b and R c together with the nitrogen to which they are bound form a heterocyclyl moiety, optionally substituted with one or more A as defined herein.
• heterocyclyl moiety in this embodiment examples include morpholinyl, thiomorpholinyl, 5,6,7,8-tetrahydro-[1,2,4]triazolo-[4,3-a]pyrazinyl, or 4,5,6,7-tetrahydro-pyrazolo[1,5-a]pyrimidinyl, each optionally substituted with one or more A as defined herein.
• R 4 is —C(O)—NR b R c , wherein R b and R c together with the nitrogen to which they are bound form a 7- to 12-membered spirocyclic heterocycle, optionally substituted with one or more A as defined herein.
• Examples for a 7-membered spirocyclic heterocycle comprise 2-oxa-6-aza-spiro[3.3]heptyl, optionally substituted with one or more A as defined herein.
• R 5 is
• R 5 is
• R 4 is as described in any of the embodiments above, R 5 is H or CF 3 , R 3 and R 6 are H, halo, CN or C 1-7 alkyl.
• R 3 , R 4 , R 5 and R 6 are not simultaneously hydrogen.
• Preferred compounds of formula I of present invention are those exemplified in examples given below. Particularly preferred are:
• the compounds of formula I and their pharmaceutically usable salts possess valuable pharmacological properties.
• Compounds of the present invention are ligands for GABA A receptors containing the ⁇ 5 subunit and are therefore useful in the therapy where cognition enhancement is required.
• the affinity of compounds at GABA A receptor subtypes was measured by competition for [3H]flumazenil (85 Ci/mmol; Roche) binding to HEK293 cells expressing rat (stably transfected) or human (transiently transfected) receptors of composition ⁇ 1 ⁇ 3 ⁇ 2, ⁇ 2 ⁇ 3 ⁇ 2, ⁇ 3 ⁇ 3 ⁇ 2 and ⁇ 5 ⁇ 3 ⁇ 2.
• Radioligand binding assays were carried out in a volume of 200 mL (96-well plates) which contained 100 mL of cell memebranes, [3H]flumazenil at a concentration of 1 nM for ⁇ 1, ⁇ 2, ⁇ 3 subunits and 0.5 nM for ⁇ 5 subunits and the test compound in the range of 10-10 ⁇ 3 ⁇ 10 ⁇ 6 M.
• Nonspecific binding was defined by 10 ⁇ 5 M diazepam and typically represented less than 5% of the total binding.
• Assays were incubated to equilibrium for 1 hour at 4° C.
• the compounds of the accompanying examples were tested in the above described assay, and the preferred compounds were found to possess a Ki value for displacement of [ 3 H]flumazenil from ⁇ 5 subunits of the rat GABA A receptor of 100 nM or less. Most preferred are compounds with a Ki (nM) ⁇ 35.
• the compounds of the invention are binding selective for the ⁇ 5 subunit relative to the ⁇ 1, ⁇ 2 and ⁇ 3 subunit.
• the present invention also provides pharmaceutical compositions containing compounds of the invention, for example, compounds of formula I or pharmaceutically acceptable salts thereof and a pharmaceutically acceptable carrier.
• Such pharmaceutical compositions can be in the form of tablets, coated tablets, dragées, hard and soft gelatin capsules, solutions, emulsions or suspensions.
• the pharmaceutical compositions also can be in the form of suppositories or injectable solutions.
• compositions of the invention in addition to one or more compounds of the invention, contain a pharmaceutically acceptable carrier.
• suitable pharmaceutically acceptable carriers include pharmaceutically inert, inorganic or organic carriers. Lactose, corn starch or derivatives thereof, talc, stearic acid or its salts etc can be used as such excipients e.g. for tablets, dragees and hard gelatine capsules.
• Suitable excipients for soft gelatine capsules are e.g. vegetable oils, waxes, fats, semisolid and liquid polyols etc.
• Suitable excipients for the manufacture of solutions and syrups are e.g. water, polyols, saccharose, invert sugar, glucose etc.
• Suitable excipients for injection solutions are e.g. water, alcohols, polyols, glycerol, vegetable oils etc.
• Suitable excipients for suppositories are e.g. natural or hardened oils, waxes, fats, semi-liquid or liquid polyols etc.
• compositions can contain preservatives, solubilizers, stabilizers, wetting agents, emulsifiers, sweeteners, colorants, flavorants, salts for varying the osmotic pressure, buffers, masking agents or antioxidants. They can also contain still other therapeutically valuable substances.
• the dosage at which compounds of the invention can be administered can vary within wide limits and will, of course, be fitted to the individual requirements in each particular case. In general, in the case of oral administration a daily dosage of about 0.1 to 1000 mg per person of a compound of general formula I should be appropriate, although the above upper limit can also be exceeded when necessary.
• Capsules of the following composition can be manufactured:
• the active substance, lactose and corn starch firstly can be mixed in a mixer and then in a comminuting machine.
• the mixture can be returned to the mixer; the talc then can be added thereto and mixed thoroughly.
• the mixture can be filled by machine into hard gelatine capsules.
• the suppository mass can melted in a glass or steel vessel, mixed thoroughly and cooled to 45° C. Thereupon, the finely powdered active substance can be added thereto and stirred until it has dispersed completely. The mixture then can be poured into suppository moulds of suitable size and left to cool; the suppositories then can be removed from the moulds and packed individually in wax paper or metal foil.
• triphenylphosphine 208 mg, 0.79 mmol
• 2-hydroxypyridine 50 mg, 0.53 mmol
• diethyl azodicarboxylate 127 ⁇ L, 0.79 mmol
• MS: m/e 267.2 [M+H] + .
• 6-(5-methyl-3-phenyl-isoxazol-4-ylmethoxy)-4-trifluoromethyl-nicotinic acid 200 mg, 0.53 mmol
• MS: m/e 420.1 [M+H] + .
• 6-(5-methyl-3-phenyl-isoxazol-4-ylmethoxy)-4-trifluoromethyl-nicotinic acid 200 mg, 0.53 mmol
• MS: m/e 462.2 [M+H] + .
• N-isopropyl-6-(5-methyl-3-phenyl-isoxazol-4-ylmethoxy)-nicotinamide 200 mg, 0.6 mmol
• 6-(5-methyl-3-phenyl-isoxazol-4-ylmethoxy)-N-(tetrahydro-pyran-4-yl)-nicotinamide was converted to the title compound (69 mg, 33%) which was obtained as a colourless gum.
• MS: m/e 408.1 [M+H] + .
• 6-(3-phenyl-5-trifluoromethyl-isoxazol-4-ylmethoxy)-nicotinic acid methyl ester 100 mg, 0.26 mmol
• 6-[3-(3-fluoro-phenyl)-5-methyl-isoxazol-4-ylmethoxy]-nicotinic acid methyl ester was converted, using methylamine (2 M in THF) instead of cyclopropylmethylamine, to the title compound (71 mg, 72%) which was obtained as a white solid.
• MS: m/e 378.4 [M+H] + .
• 6-(3-phenyl-5-trifluoromethyl-isoxazol-4-ylmethoxy)-nicotinic acid methyl ester 100 mg, 0.26 mmol
• 6-[3-(4-chloro-phenyl)-5-methyl-isoxazol-4ylmethoxy]-nicotinic acid methyl ester 144 mg, 0.4 mmol
• ethylamine (2 M in THF) instead of 2,2,2-trifluoroethylamine
• 6-(3-phenyl-5-trifluoromethyl-isoxazol-4-ylmethoxy)-nicotinic acid methyl ester 100 mg, 0.26 mmol
• 6-[3-(3-fluoro-phenyl)-5-methyl-isoxazol-4-ylmethoxy]-nicotinic acid methyl ester was converted, using 4-aminotetrahydropyran instead of cyclopropylmethylamine, to the title compound (111 mg, 94%) which was obtained as a white solid.
• MS: m/e 448.3 [M+H] + .
• the reaction mixture was treated with a aqueous sodium hydroxide (1 N, 15.8 mL, 15.8 mmol) and stirred for 0.5 h at 70° C.
• the solution was cooled to ambient temperature, diluted with water (15 mL) and washed with tert-butylmethylether (15 mL).
• 6-(5-methyl-3-pyridin-4-yl-isoxazol-4-ylmethoxy)-nicotinic acid 200 mg, 0.64 mol
• 6-(5-methyl-3-phenyl-isoxazol-4-ylmethoxy)-nicotinic acid was converted using isopropylamine instead of 2,2,2-trifluoroethylamine to the title compound (trituration with tert-butylmethylether, 158 mg, 70%) which was obtained as a white solid.
• MS: m/e 353.3 [M+H] + .
• 6-(5-methyl-3-pyridin-4-yl-isoxazol-4-ylmethoxy)-nicotinic acid 200 mg, 0.64 mol
• 6-(5-methyl-3-phenyl-isoxazol-4-ylmethoxy)-nicotinic acid was converted using 4-aminotetrahydropyran instead of 2,2,2-trifluoroethylamine to the title compound (trituration with tert-butylmethylether, 178 mg, 70%) which was obtained as a white solid.
• MS: m/e 395.2 [M+H] + .
• 6-(5-methyl-3-pyridin-3-yl-isoxazol-4-ylmethoxy)-nicotinic acid methyl ester 130 mg, 0.4 mmol
• 6-[3-(3-fluoro-phenyl)-5-methyl-isoxazol-4-ylmethoxy]-nicotinic acid methyl ester was converted, using 2,2,2-trifluoroethylamine instead of cyclopropylamine, to the title compound (139 mg, 89%) which was obtained as an off white solid.
• MS: m/e 393.1 [M+H] + .
• 6-(5-methyl-3-pyridin-3-yl-isoxazol-4-ylmethoxy)-nicotinic acid methyl ester 130 mg, 0.4 mmol
• 6-[3-(3-fluoro-phenyl)-5-methyl-isoxazol-4-ylmethoxy]-nicotinic acid methyl ester was converted to the title compound (98 mg, 67%) which was obtained as a light yellow solid.
• MS: m/e 365.1 [M+H] + .
• 6-(5-methyl-3-pyridin-3-yl-isoxazol-4-ylmethoxy)-nicotinic acid methyl ester 130 mg, 0.4 mmol
• 6-[3-(3-fluoro-phenyl)-5-methyl-isoxazol-4-ylmethoxy]-nicotinic acid methyl ester using cyclopropylamine instead of 2,2,2-trifluoroethylamine, was converted to the title compound (117 mg, 83%) which was obtained as an off white solid.
• MS: m/e 351.4 [M+H] + .
• 6-(5-methyl-3-pyridin-3-yl-isoxazol-4-ylmethoxy)-nicotinic acid methyl ester 130 mg, 0.4 mmol
• 6-[3-(3-fluoro-phenyl)-5-methyl-isoxazol-4-ylmethoxy]-nicotinic acid methyl ester using 4-aminotetrahydropyran instead of 2,2,2-trifluoroethylamine, was converted to the title compound (117 mg, 83%) which was obtained as an off white solid.
• MS: m/e 395.1 [M+H] + .

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• 2008
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