US20010006973A1 - 1-oxo- and 1,3-dioxoisoindolines and method of reducing inflammatory cytokine levels - Google Patents

1-oxo- and 1,3-dioxoisoindolines and method of reducing inflammatory cytokine levels Download PDF

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US20010006973A1
US20010006973A1 US09/270,411 US27041199A US2001006973A1 US 20010006973 A1 US20010006973 A1 US 20010006973A1 US 27041199 A US27041199 A US 27041199A US 2001006973 A1 US2001006973 A1 US 2001006973A1
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compound according
dioxo
dioxopiperidin
chirally pure
substantially chirally
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Hon-Wah Man
George W. Muller
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Priority to US09/655,571 priority patent/US6403613B1/en
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Priority to US11/236,740 priority patent/US7820697B2/en
Priority to US11/827,802 priority patent/US20080287496A1/en
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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D401/00—Heterocyclic compounds containing two or more hetero rings, having nitrogen atoms as the only ring hetero atoms, at least one ring being a six-membered ring with only one nitrogen atom
    • C07D401/02—Heterocyclic compounds containing two or more hetero rings, having nitrogen atoms as the only ring hetero atoms, at least one ring being a six-membered ring with only one nitrogen atom containing two hetero rings
    • C07D401/04—Heterocyclic compounds containing two or more hetero rings, having nitrogen atoms as the only ring hetero atoms, at least one ring being a six-membered ring with only one nitrogen atom containing two hetero rings directly linked by a ring-member-to-ring-member bond
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    • A61K31/395—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins
    • A61K31/435—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having six-membered rings with one nitrogen as the only ring hetero atom
    • A61K31/44—Non condensed pyridines; Hydrogenated derivatives thereof
    • A61K31/445—Non condensed piperidines, e.g. piperocaine
    • A61K31/4523—Non condensed piperidines, e.g. piperocaine containing further heterocyclic ring systems
    • A61K31/454—Non condensed piperidines, e.g. piperocaine containing further heterocyclic ring systems containing a five-membered ring with nitrogen as a ring hetero atom, e.g. pimozide, domperidone
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    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
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    • A—HUMAN NECESSITIES
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Definitions

  • TNF ⁇ Tumor necrosis factor- ⁇
  • TNF ⁇ is a cytokine which is released primarily by mononuclear phagocytes in response to a number immunostimulators. It is a key proinflammatory cytokine in the inflammation cascade causing the production and/or release of other cytokines and agents. When administered to animals or humans, it causes inflammation, fever, cardiovascular effects, hemorrhage, coagulation, and acute phase responses similar to those seen during acute infections and shock states. Excessive or unregulated TNF ⁇ production thus has been implicated in a number of disease conditions.
  • TNF ⁇ appears to be involved in bone resorption diseases, including arthritis. When activated, leukocytes will produce bone-resorption, an activity to which the data suggest TNF ⁇ contributes. ⁇ Bertolini et al., Nature 319, 516-518 (1986) and Johnson et al., Endocrinology 124(3), 1424-1427 (1989). ⁇ TNF ⁇ also has been shown to stimulate bone resorption and inhibit bone formation in vitro and in vivo through stimulation of osteoclast formation and activation combined with inhibition of osteoblast function. Although TNF ⁇ may be involved in many bone resorption diseases, including arthritis, the most compelling link with disease is the association between production of TNF ⁇ by tumor or host tissues and malignancy associated hypercalcemia ⁇ Calci.
  • Cerebral malaria is a lethal hyperacute neurological syndrome associated with high blood levels of TNF ⁇ and the most severe complication occurring in malaria patients. Levels of serum TNF ⁇ correlated directly with the severity of disease and the prognosis in patients with acute malaria attacks ⁇ Grau et al., N. Engl. J. Med. 320(24), 1586-1591 (1989) ⁇ .
  • Macrophage-induced angiogenesis is known to be mediated by TNF ⁇ .
  • Leibovich et al. ⁇ Nature, 329, 630-632 (1987) ⁇ showed TNF ⁇ induces in vivo capillary blood vessel formation in the rat cornea and the developing chick chorioallantoic membranes at very low doses and suggest TNF ⁇ is a candidate for inducing angiogenesis in inflammation, wound repair, and tumor growth.
  • TNF ⁇ production also has been associated with cancerous conditions, particularly induced tumors ⁇ Ching et al., Brit. J. Cancer, (1955) 72, 339-343, and Koch, Progress in Medicinal Chemistry, 22, 166-242 (1985) ⁇ .
  • TNF ⁇ also plays a role in the area of chronic pulmonary inflammatory diseases.
  • the deposition of silica particles leads to silicosis, a disease of progressive respiratory failure caused by a fibrotic reaction.
  • Antibody to TNF ⁇ completely blocked the silica-induced lung fibrosis in mice ⁇ Pignet et al, Nature, 344, 245-247 (1990) ⁇ .
  • High levels of TNF ⁇ production have been demonstrated in animal models of silica and asbestos induced fibrosis ⁇ Bissonnette et al., Inflammation 13(3), 329-339 (1989) ⁇ .
  • TNF ⁇ is also implicated in the inflammatory response which follows reperfusion, called reperfusion injury, and is a major cause of tissue damage after loss of blood flow ⁇ Vedder et al., PNAS 87, 2643-2646 (1990) ⁇ .
  • TNF ⁇ also alters the properties of endothelial cells and has various pro-coagulant activities, such as producing an increase in tissue factor pro-coagulant activity and suppression of the anticoagulant protein C pathway as well as down-regulating the expression of thrombomodulin ⁇ Sherry et al., J. Cell Biol. 107, 1269-1277 (1988) ⁇ .
  • TNF ⁇ has pro-inflammatory activities which together with its early production (during the initial stage of an inflammatory event) make it a likely mediator of tissue injury in several important disorders including but not limited to, myocardial infarction, stroke and circulatory shock.
  • TNF ⁇ -induced expression of adhesion molecules such as intercellular adhesion molecule (ICAM) or endothelial leukocyte adhesion molecule (ELAM) on endothelial cells ⁇ Munro et al., Am. J. Path. 135(1), 121-132 (1989) ⁇ .
  • IAM intercellular adhesion molecule
  • ELAM endothelial leukocyte adhesion molecule
  • TNF ⁇ blockage with monoclonal anti-TNF ⁇ antibodies has been shown to be beneficial in rheumatoid arthritis ⁇ Elliot et al., Int. J. Pharmac. 1995 17(2), 141-145 ⁇ .
  • High levels of TNF ⁇ are associated with Crohn's disease ⁇ von Dullemen et al., Gastroenterology, 1995 109(1), 129-135 ⁇ and clinical benefit has been achieved with TNF ⁇ antibody treatment.
  • TNF ⁇ is a potent activator of retrovirus replication including activation of HIV-1.
  • HIV Human Immunodeficiency Virus
  • HIV-1 HIV-1
  • HIV-2 HIV-2
  • HIV-3 HIV-3
  • T-cell mediated immunity is impaired and infected individuals manifest severe opportunistic infections and/or unusual neoplasms.
  • HIV entry into the T lymphocyte requires T lymphocyte activation.
  • Other viruses, such as HIV-1, HIV-2 infect T lymphocytes after T cell activation and such virus protein expression and/or replication is mediated or maintained by such T cell activation.
  • the T lymphocyte must continue to be maintained in an activated state to permit HIV gene expression and/or HIV replication.
  • Cytokines are implicated in activated T-cell mediated HIV protein expression and/or virus replication by playing a role in maintaining T lymphocyte activation. Therefore, interference with cytokine activity such as by prevention or inhibition of cytokine production, notably TNF ⁇ , in an HIV-infected individual assists in limiting the maintenance of T lymphocyte caused by HIV infection.
  • Monocytes, macrophages, and related cells have been implicated in maintenance of the HIV infection. These cells, like T cells, are targets for viral replication and the level of viral replication is dependent upon the activation state of the cells. ⁇ Rosenberg et al., The Immunopathogenesis of HIV Infection, Advances in Immunology, 57 (1989) ⁇ . Cytokines, such as TNF ⁇ , have been shown to activate HIV replication in monocytes and/or macrophages ⁇ Poli et al., Proc. Natl. Acad.
  • TNF ⁇ cytokine production or activity aids in limiting HIV progression for T cells.
  • Additional studies have identified TNF ⁇ as a common factor in the activation of HIV in vitro and has provided a clear mechanism of action via a nuclear regulatory protein found in the cytoplasm of cells (Osborn, et al., PNAS 86 2336-2340). This evidence suggests that a reduction of TNF ⁇ synthesis may have an antiviral effect in HIV infections, by reducing the transcription and thus virus production.
  • AIDS viral replication of latent HIV in T cell and macrophage lines can be induced by TNF ⁇ ⁇ Folks et al., PNAS 86, 2365-2368 (1989) ⁇ .
  • a molecular mechanism for the virus inducing activity is suggested by TNF ⁇ 's ability to activate a gene regulatory protein (NF ⁇ B) found in the cytoplasm of cells, which promotes HIV replication through binding to a viral regulatory gene sequence (LTR) ⁇ Osborn et al., PNAS 86, 2336-2340 (1989) ⁇ .
  • TNF ⁇ in AIDS associated cachexia is suggested by elevated serum TNF ⁇ and high levels of spontaneous TNF ⁇ production in peripheral blood monocytes from patients ⁇ Wright et al., J. Immunol.
  • TNF ⁇ has been implicated in various roles with other viral infections, such as the cytomegalia virus (CMV), influenza virus, adenovirus, and the herpes family of viruses for similar reasons as those noted.
  • CMV cytomegalia virus
  • influenza virus influenza virus
  • adenovirus adenovirus
  • herpes family of viruses for similar reasons as those noted.
  • NF ⁇ B nuclear factor ⁇ B
  • NF ⁇ B nuclear factor ⁇ B
  • NF ⁇ B pleiotropic transcriptional activator
  • NF ⁇ B has been implicated as a transcriptional activator in a variety of disease and inflammatory states and is thought to regulate cytokine levels including but not limited to TNF ⁇ and also to be an activator of HIV transcription (Dbaibo, et al., J. Biol. Chem. 1993, 17762-66; Duh et al., Proc. Natl. Acad. Sci. 1989, 86, 5974-78; Bachelerie et al., Nature 1991, 350, 709-12; Boswas et al., J.
  • TNF ⁇ and NF ⁇ B levels are influenced by a reciprocal feedback loop. As noted above, the compounds of the present invention affect the levels of both TNF ⁇ and NF ⁇ B.
  • cAMP adenosine 3′,5′-cyclic monophosphate
  • TNF ⁇ levels and/or increasing cAMP levels thus constitutes a valuable therapeutic strategy for the treatment of many inflammatory, infectious, immunological or malignant diseases.
  • diseases include but are not restricted to septic shock, sepsis, endotoxic shock, hemodynamic shock and sepsis syndrome, post ischemic reperfusion injury, malaria, mycobacterial infection, meningitis, psoriasis, congestive heart failure, fibrotic disease, cachexia, graft rejection, cancer, autoimmune disease, opportunistic infections in AIDS, rheumatoid arthritis, rheumatoid spondylitis, osteoarthritis, other arthritic conditions, Crohn's disease, ulcerative colitis, multiple sclerosis, systemic lupus erythrematosis, ENL in leprosy, radiation damage, and hyperoxic alveolar injury.
  • the present invention is based on the discovery that certain classes of non-polypeptide compounds more fully described herein decrease the levels of TNF ⁇ , increase cAMP levels, and inhibit inflammatory cytokines.
  • the present invention thus relates to 1-oxo- and 1,3-dioxo-2-(2,6-dioxopiperidin-3-yl)isoindolines substituted in the 4-position of the isoindoline ring and optionally further substituted in the 3-position of the 2,6-dioxopiperidine ring , the method of reducing levels of tumor necrosis factor ⁇ and other inflammatory cytokines in a mammal through the administration of such derivatives, and pharmaceutical compositions containing such derivatives.
  • the invention pertains to
  • Y is oxygen or H 2 .
  • a first of R 1 and R 2 is halo, alkyl, alkoxy, alkylamino, dialkylamino, cyano, or carbamoyl
  • the second of R 1 and R 2 independently of the first, is hydrogen, halo, alkyl, alkoxy, alkylamino, dialkylamino, cyano, or carbamoyl
  • R 3 is hydrogen, alkyl, or benzyl
  • alkyl denotes a univalent saturated branched or straight hydrocarbon chain containing from 1 to 4 carbon atoms.
  • alkyl groups are methyl, ethyl, propyl, isopropyl, butyl, isobutyl, sec-butyl, and tert-butyl.
  • Alkoxy refers to an alkyl group bound to the remainder of the molecule through an ethereal oxygen atom.
  • Representative of such alkoxy groups are methoxy, ethoxy, propoxy, isopropoxy, butoxy, isobutoxy, sec-butoxy, and tert-butoxy.
  • Halo includes bromo, chloro, fluoro, and iodo.
  • the compounds of Formula I are used, under the supervision of qualified professionals, to inhibit the undesirable effects of TNF ⁇ and other inflammatory cytokines including the interleukins IL-1, IL-6, and IL-12.
  • the compounds can be administered orally, rectally, or parenterally, alone or in combination with other therapeutic agents including antibiotics, steroids, chemotherapeutic agents, etc., to a mammal in need of treatment; e.g., in the treatment of cancers, rheumatoid arthritis, inflammatory bowel disease, muscular dystrophy, Crohn's disease, etc.
  • the compounds of the present invention also can be used topically in the treatment or prophylaxis of disease states mediated or exacerbated by excessive TNF ⁇ production, respectively, such as viral infections, such as those caused by the herpes viruses, or viral conjunctivitis, psoriasis, atopic dermatitis, etc.
  • TNF ⁇ mediated diseases for treatment, therapeutically or prophylactically, in animals include disease states such as those noted above, but in particular viral infections. Examples include feline immunodeficiency virus, equine infectious anaemia virus, caprine arthritis virus, visna virus, and maedi virus, as well as other lentiviruses.
  • the compounds of Formula I are readily prepared through a number of routes.
  • an anhydride or lactone is allowed to react with a 3-amino-2,6-dioxopiperidine:
  • each of R 1 , R 2 , R 3 , and Y are as defined above.
  • the 3-amino-2,6-dioxopiperidine can be obtained from the corresponding glutamic acid anhydride through conventional amidation or from the cyclization of appropriate glutamine derivatives.
  • R 4 is CHO or CH 2 Br in the presence of an acid acceptor such as dimethylaminopyridine or triethylamine.
  • disubstituted benzoate intermediates are known or can be obtained though conventional processes.
  • a lower alkyl ester of a 3,6-disubstituted ortho-toluic acid is brominated with N-bromosuccinimide under the influence of light to yield the lower alkyl 2-(bromomethyl)-3,6-disubstitutedbenzoate.
  • racemates of these isomers and the individual isomers themselves, as well as diastereomers when a second chiral center is present are within the scope of the present invention.
  • the racemates can be used as such or can be separated into their individual isomers mechanically as by chromatography using a chiral absorbent.
  • the individual isomers can be prepared in chiral form or separated chemically from a mixture by forming salts with a chiral acid or base, such as the individual enantiomers of 10-camphorsulfonic acid, camphoric acid, ⁇ -bromocamphoric acid, methoxyacetic acid, tartaric acid, diacetyltartaric acid, malic acid, pyrrolidone-5-carboxylic acid, and the like, and then freeing one or both of the resolved bases, optionally repeating the process, so as obtain either or both substantially free of the other; i.e., in a form having an optical purity of >95%.
  • a chiral acid or base such as the individual enantiomers of 10-camphorsulfonic acid, camphoric acid, ⁇ -bromocamphoric acid, methoxyacetic acid, tartaric acid, diacetyltartaric acid, malic acid, pyrrolidone-5-carboxylic acid, and the like,
  • the present invention also pertains to the physiologically acceptable non-toxic acid addition salts of the compound of Formula I which contain a group capable of being protonated; e.g., amino.
  • Such salts include those derived from organic and inorganic acids such as, without limitation, hydrochloric acid, hydrobromic acid, phosphoric acid, sulfuric acid, methanesulphonic acid, acetic acid, tartaric acid, lactic acid, succinic acid, citric acid, malic acid, maleic acid, sorbic acid, aconitic acid, salicylic acid, phthalic acid, embonic acid, enanthic acid, and the like.
  • Particularly preferred compounds include 1,3-dioxo-2-(2,6-dioxopiperidin-3-yl)-4-methylisoindoline, 1,3-dioxo-2-(2,6-dioxopiperidin-3-yl)-4-ethylisoindoline, 1,3-dioxo-2-(2,6-dioxo-3-methylpiperidin-3-yl)-4-methylisoindoline, 1,3-dioxo-2-(2,6-dioxoopiperidin-3-yl)-4,7-dimethylisoindoline, 1-oxo-2-(2,6-dioxo-3-methylpiperidin-3-yl)-4-ethylisoindoline, 1-oxo-2-(2,6-dioxo-3-methylpiperidin-3-yl)-4-methylisoindoline, 1-oxo-2-(2,6-dioxoxo-3
  • Oral dosage forms include tablets, capsules, dragees, and similar shaped, compressed pharmaceutical forms containing from 1 to 100 mg of drug per unit dosage.
  • Isotonic saline solutions containing from 20 to 100 mg/mL can be used for parenteral administration which includes intramuscular, intrathecal, intravenous and intra-arterial routes of administration. Rectal administration can be effected through the use of suppositories formulated from conventional carriers such as cocoa butter.
  • compositions thus comprise one or more compounds of Formulas I associated with at least one pharmaceutically acceptable carrier, diluent or excipient.
  • the active ingredients are usually mixed with or diluted by an excipient or enclosed within such a carrier which can be in the form of a capsule or sachet.
  • the excipient serves as a diluent, it may be a solid, semi-solid, or liquid material which acts as a vehicle, carrier, or medium for the active ingredient.
  • compositions can be in the form of tablets, pills, powders, elixirs, suspensions, emulsions, solutions, syrups, soft and hard gelatin capsules, suppositories, sterile injectable solutions and sterile packaged powders.
  • excipients examples include lactose, dextrose, sucrose, sorbitol, mannitol, starch, gum acacia, calcium silicate, microcrystalline cellulose, polyvinylpyrrolidinone, cellulose, water, syrup, and methyl cellulose
  • the formulations can additionally include lubricating agents such as talc, magnesium stearate and mineral oil, wetting agents, emulsifying and suspending agents, preserving agents such as methyl- and propylhydroxybenzoates, sweetening agents or flavoring agents.
  • compositions preferably are formulated in unit dosage form, meaning physically discrete units suitable as a unitary dosage, or a predetermined fraction of a unitary dose to be administered in a single or multiple dosage regimen to human subjects and other mammals, each unit containing a predetermined quantity of active material calculated to produce the desired therapeutic effect in association with a suitable pharmaceutical excipient.
  • the compositions can be formulated so as to provide an immediate, sustained or delayed release of active ingredient after administration to the patient by employing procedures well known in the art.
  • Enzyme-linked immunosorbent assays for TNF ⁇ can be performed in a conventional manner.
  • PBMC is isolated from normal donors by Ficoll-Hypaque density centrifugation. Cells are cultured in RPMI supplemented with 10% AB+ serum, 2 mM L-glutamine, 100 U/mL penicillin, and 100 mg/mL streptomycin. Drugs are dissolved in dimethylsulfoxide (Sigma Chemical) and further dilutions are done in supplemented RPMI. The final dimethylsulfoxide concentration in the presence or absence of drug in the PBMC suspensions is 0.25 wt %. Drugs are assayed at half-log dilutions starting at 50 mg/mL.
  • PBMC PBMC (10 6 cells/mL) in 96 wells plates one hour before the addition of LPS.
  • PBMC (10 6 cells/mL) in the presence or absence of drug are stimulated by treatment with 1 mg/mL of LPS from Salmonella minnesota R595 (List Biological Labs, Campbell, Calif.). Cells are then incubated at 37° C. for 18-20 hours. Supernatants are harvested and assayed immediately for TNF ⁇ levels or kept frozen at ⁇ 70° C. (for not more than 4 days) until assayed. The concentration of TNF ⁇ in the supernatant is determined by human TNF ⁇ ELISA kits (ENDOGEN, Boston, Mass.) according to the manufacturer's directions.
  • a mixture of 16.25 g of 2,6-dioxopiperidin-3-ammonium chloride, and 30.1 g of methyl 2-bromomethyl-3-methylbenzoate, and 12.5 g of triethylamine in 100 mL of dimethylformamide is stirred at room temperature for 15 hours.
  • the mixture is then concentrated in vacuo and the residue mixed with methylene chloride and water.
  • the aqueous layer is separated and back-extracted with methylene chloride.
  • the combined methylene chloride solutions are dried over magnesium sulfate and concentrated in vacuo to give 1-oxo-2-(2,6-dioxopiperidin-3-yl)-4-methylisoindoline.
  • 2-(2,6-Dioxopiperid-3-yl)-4,7-dimethylisoindoline-1,3-dione was prepared by the procedure of Example 1 from 3,6-dimethylphthalic anhydride (220 mg, 1.25 mmol), 3-aminopiperidine-2,6-dione hydrogen chloride (204 mg, 1.24 mmol) and sodium acetate (10 mg, 1.34 mmol) in acetic acid (10 mL).
  • 2-(2,6-Dioxo(3-piperidyl))-4-ethylisoindoline-1,3-dione was prepared by the procedure of Example 1 from 3-ethylphthalic anhydride (0.860 g, 4.89 mmol), 3-aminopiperidine-2,6-dione hydrogen chloride (0.803 g, 4.88 mmol) and sodium acetate (0.420 g, 5.12 mmol) in acetic acid (10 mL).
  • Tablets each containing 50 mg of 1-oxo-2-(2,6-dioxopiperidin-3-yl)-4-methylisoindoline, can be prepared in the following manner: Constituents (for 1000 tablets) 1-oxo-2-(2,6-dioxo-piperidin- 50.0 g 3-yl)-4-methyl-isoindoline lactose 50.7 g wheat starch 7.5 g polyethylene glycol 6000 5.0 g talc 5.0 g magnesium stearate 1.8 g demineralized water q.s.
  • the solid ingredients are first forced through a sieve of 0.6 mm mesh width.
  • the active ingredient, lactose, talc, magnesium stearate and half of the starch then are mixed.
  • the other half of the starch is suspended in 40 mL of water and this suspension is added to a boiling solution of the polyethylene glycol in 100 mL of water.
  • the resulting paste is added to the pulverulent substances and the mixture is granulated, if necessary with the addition of water.
  • the granulate is dried overnight at 35° C., forced through a sieve of 1.2 mm mesh width and compressed to form tablets of approximately 6 mm diameter which are concave on both sides.
  • Gelatin dry-filled capsules each containing 100 mg of 1,3-dioxo-2-(2,6-dioxopiperidin-3-yl)-4-methylisoindoline, can be prepared in the following manner: Composition (for 1000 capsules) 1,3-dioxo-2-(2,6-dioxo- 100.0 g piperidin-3-yl)-4-methyl- isoindoline microcrystalline cellulose 30.0 g sodium lauryl sulfate 2.0 g magnesium stearate 8.0 g
  • the sodium lauryl sulfate is sieved into the 1,3-dioxo-2-(2,6-dioxopiperidin-3-yl)-4-methylisoindoline through a sieve of 0.2 mm mesh width and the two components are intimately mixed for 10 minutes.
  • the microcrystalline cellulose is then added through a sieve of 0.9 mm mesh width and the whole is again intimately mixed for 10 minutes.
  • the magnesium stearate is added through a sieve of 0.8 mm width and, after mixing for a further 3 minutes, the mixture is introduced in portions of 140 mg each into size 0 (elongated) gelatin dry-fill capsules.
  • a 0.2% injection or infusion solution can be prepared, for example, in the following manner: 1,3-dioxo-2-(2,6-dioxopiperidin- 5.0 g 3-yl)-4,7-dimethylisoindoline sodium chloride 22.5 g phosphate buffer pH 7.4 300.0 g demineralized water to 2500.0 mL

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