UA103771C2 - Стрептокиназа, активирующая плазмин, способ и набор для получения плазмина - Google Patents

Стрептокиназа, активирующая плазмин, способ и набор для получения плазмина

Info

Publication number
UA103771C2
UA103771C2 UAA201015856A UAA201015856A UA103771C2 UA 103771 C2 UA103771 C2 UA 103771C2 UA A201015856 A UAA201015856 A UA A201015856A UA A201015856 A UAA201015856 A UA A201015856A UA 103771 C2 UA103771 C2 UA 103771C2
Authority
UA
Ukraine
Prior art keywords
plasmin
streptokinase
activating
production
immobilized
Prior art date
Application number
UAA201015856A
Other languages
English (en)
Ukrainian (uk)
Inventor
Эдвард КЕПФ
Томас П. ЦИММЕРМАН
Original Assignee
Грифолз Терапьютикс Инк.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Грифолз Терапьютикс Инк. filed Critical Грифолз Терапьютикс Инк.
Publication of UA103771C2 publication Critical patent/UA103771C2/ru

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/315—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Streptococcus (G), e.g. Enterococci
    • C07K14/3153—Streptokinase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
    • C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
    • C12N11/10—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a carbohydrate
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/6435—Plasmin (3.4.21.7), i.e. fibrinolysin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6489—Metalloendopeptidases (3.4.24)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C12Y304/21007—Plasmin (3.4.21.7), i.e. fibrinolysin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/24—Metalloendopeptidases (3.4.24)
    • C12Y304/24029—Aureolysin (3.4.24.29)

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

Изобретение относится к стрептокиназе, иммобилизированной на матрице, которая активирует плазминоген в плазмин, при этом, оставаясь стойкой к расщеплению плазмином по сравнению с соответствующей ей стрептокиназой дикого типа, содержащей аминокислотную последовательность, которая имеет аспарагин в положениях, которые отвечают положениям 85 и 412 в SEQ ID N0:1, и к способу получения плазмина, который включает контактирование стрептокиназы, иммобилизированной на матрице или матрице, которая имеет иммобилизированную на ней стрептокиназу, с плазминогеном, таким образом, превращая плазминоген в плазмин; и b) очистку плазмина, а также к набору для получения плазмина.
UAA201015856A 2008-06-04 2009-06-03 Стрептокиназа, активирующая плазмин, способ и набор для получения плазмина UA103771C2 (ru)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US5867708P 2008-06-04 2008-06-04
PCT/US2009/046152 WO2009149199A2 (en) 2008-06-04 2009-06-03 Composition, method and kit for preparing plasmin

Publications (1)

Publication Number Publication Date
UA103771C2 true UA103771C2 (ru) 2013-11-25

Family

ID=41398848

Family Applications (1)

Application Number Title Priority Date Filing Date
UAA201015856A UA103771C2 (ru) 2008-06-04 2009-06-03 Стрептокиназа, активирующая плазмин, способ и набор для получения плазмина

Country Status (15)

Country Link
US (1) US8617863B2 (ru)
EP (1) EP2297317A4 (ru)
JP (1) JP2011522538A (ru)
KR (2) KR20160110544A (ru)
CN (1) CN102057044B (ru)
AU (1) AU2009256168B2 (ru)
BR (1) BRPI0913397B8 (ru)
CA (1) CA2726908C (ru)
IL (1) IL209511A (ru)
MX (1) MX2010013282A (ru)
NZ (1) NZ589557A (ru)
RU (1) RU2497948C2 (ru)
UA (1) UA103771C2 (ru)
WO (1) WO2009149199A2 (ru)
ZA (1) ZA201008564B (ru)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102234332B (zh) * 2010-04-26 2014-12-17 浙江海正药业股份有限公司 一种重组人血白蛋白及其融合蛋白的分离纯化工艺
FR3034992A1 (fr) * 2015-04-15 2016-10-21 Arcadophta Milieu plasmatique ex vivo autologue riche en plasmine et procede d'obtention
FR3035120B1 (fr) * 2015-04-15 2020-02-07 Arcadophta Composition de plasminogenase immobilisee, procede de preparation, utilisation et dispositif comprenant une telle composition

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Also Published As

Publication number Publication date
IL209511A0 (en) 2011-01-31
WO2009149199A3 (en) 2010-04-01
EP2297317A4 (en) 2011-12-07
CN102057044B (zh) 2013-05-01
RU2497948C2 (ru) 2013-11-10
MX2010013282A (es) 2010-12-21
JP2011522538A (ja) 2011-08-04
IL209511A (en) 2015-07-30
BRPI0913397A2 (pt) 2015-08-04
KR20160110544A (ko) 2016-09-21
BRPI0913397B1 (pt) 2020-02-04
CN102057044A (zh) 2011-05-11
WO2009149199A2 (en) 2009-12-10
NZ589557A (en) 2012-08-31
ZA201008564B (en) 2011-07-27
US8617863B2 (en) 2013-12-31
AU2009256168A1 (en) 2009-12-10
CA2726908C (en) 2017-01-24
KR20110017903A (ko) 2011-02-22
BRPI0913397B8 (pt) 2021-05-25
RU2010154401A (ru) 2012-07-20
CA2726908A1 (en) 2009-12-10
EP2297317A2 (en) 2011-03-23
AU2009256168B2 (en) 2014-06-05
US20110201077A1 (en) 2011-08-18

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