DK1343903T3 - En gærekspressionsvektor og en fremgangsmåde til fremstilling af et rekombinant protein ved ekspression i en gærcelle - Google Patents

En gærekspressionsvektor og en fremgangsmåde til fremstilling af et rekombinant protein ved ekspression i en gærcelle

Info

Publication number
DK1343903T3
DK1343903T3 DK01271444T DK01271444T DK1343903T3 DK 1343903 T3 DK1343903 T3 DK 1343903T3 DK 01271444 T DK01271444 T DK 01271444T DK 01271444 T DK01271444 T DK 01271444T DK 1343903 T3 DK1343903 T3 DK 1343903T3
Authority
DK
Denmark
Prior art keywords
expression
yeast
producing
recombinant protein
expression vector
Prior art date
Application number
DK01271444T
Other languages
English (en)
Inventor
Desire Jose Collen
Nubuo Nagai
Yves Laroche
Original Assignee
Thrombogenics Nv
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from GB0031196A external-priority patent/GB0031196D0/en
Priority claimed from GB0116690A external-priority patent/GB0116690D0/en
Priority claimed from GB0116702A external-priority patent/GB0116702D0/en
Application filed by Thrombogenics Nv filed Critical Thrombogenics Nv
Application granted granted Critical
Publication of DK1343903T3 publication Critical patent/DK1343903T3/da

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/6435—Plasmin (3.4.21.7), i.e. fibrinolysin
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P7/00—Drugs for disorders of the blood or the extracellular fluid
    • A61P7/02—Antithrombotic agents; Anticoagulants; Platelet aggregation inhibitors
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P9/00—Drugs for disorders of the cardiovascular system
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P9/00—Drugs for disorders of the cardiovascular system
    • A61P9/10—Drugs for disorders of the cardiovascular system for treating ischaemic or atherosclerotic diseases, e.g. antianginal drugs, coronary vasodilators, drugs for myocardial infarction, retinopathy, cerebrovascula insufficiency, renal arteriosclerosis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C12Y304/21007—Plasmin (3.4.21.7), i.e. fibrinolysin

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Chemical & Material Sciences (AREA)
  • Animal Behavior & Ethology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Heart & Thoracic Surgery (AREA)
  • Cardiology (AREA)
  • Molecular Biology (AREA)
  • Diabetes (AREA)
  • Urology & Nephrology (AREA)
  • Vascular Medicine (AREA)
  • Hematology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Peptides Or Proteins (AREA)
DK01271444T 2000-12-21 2001-12-20 En gærekspressionsvektor og en fremgangsmåde til fremstilling af et rekombinant protein ved ekspression i en gærcelle DK1343903T3 (da)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
GB0031196A GB0031196D0 (en) 2000-12-21 2000-12-21 Recombinant human plasminogen and plasmin: methods for high yield production stabilization and use for treatment
GB0116690A GB0116690D0 (en) 2001-07-09 2001-07-09 Human plasminogen and plasmin
GB0116702A GB0116702D0 (en) 2001-07-09 2001-07-09 Human plasminogen and plasmin
PCT/BE2001/000217 WO2002050290A1 (en) 2000-12-21 2001-12-20 A yeast expression vector and a method of making a recombinant protein by expression in a yeast cell

Publications (1)

Publication Number Publication Date
DK1343903T3 true DK1343903T3 (da) 2008-03-10

Family

ID=27256020

Family Applications (1)

Application Number Title Priority Date Filing Date
DK01271444T DK1343903T3 (da) 2000-12-21 2001-12-20 En gærekspressionsvektor og en fremgangsmåde til fremstilling af et rekombinant protein ved ekspression i en gærcelle

Country Status (11)

Country Link
US (1) US7445775B2 (da)
EP (1) EP1343903B1 (da)
JP (2) JP4047170B2 (da)
AT (1) ATE378413T1 (da)
AU (1) AU2002218890A1 (da)
CY (1) CY1107866T1 (da)
DE (1) DE60131450T2 (da)
DK (1) DK1343903T3 (da)
ES (1) ES2296705T3 (da)
PT (1) PT1343903E (da)
WO (1) WO2002050290A1 (da)

Families Citing this family (22)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6998121B2 (en) * 2003-01-23 2006-02-14 Milkhaus Laboratory, Inc. Method of treatment of connective tissue disorders by administration of streptolysin O
MXPA04007585A (es) * 2002-02-06 2005-09-20 N Zyme Biotec Gmbh Metodo para la produccion de proteinas recombinantes en microorganismos.
US20050124036A1 (en) * 2002-02-06 2005-06-09 Rudy Susilo Method for producing recombinant proteins in micro-organisms
GB0228409D0 (en) * 2002-12-06 2003-01-08 Thromb X Nv Pharmacological vitreolysis
KR100595864B1 (ko) * 2004-01-27 2006-06-30 재단법인 목암생명공학연구소 형질전환 사카로마이세스 세레비지애 균주 및 이를 이용한ｌｋ8 단백질의 생산방법
WO2005078109A2 (en) 2004-02-11 2005-08-25 Biolex, Inc. Expression of plasminogen and microplasminogen in duckweed
DK1740698T3 (da) * 2004-04-22 2010-10-25 Talecris Biotherapeutics Inc Rekombinant modificeret plasmin
CN100368524C (zh) * 2005-05-17 2008-02-13 上海大学 巴士德毕赤酵母gs115/pfk-k5的高密度发酵方法
US20070134231A1 (en) * 2005-12-13 2007-06-14 Jani Dharmendra M Method for prolonging activity of autodegradable enzymes and compositions thereof
US20070134230A1 (en) * 2005-12-13 2007-06-14 Jani Dharmendra M Method for prolonging activity of autodegradable enzymes
US20070196350A1 (en) * 2006-02-22 2007-08-23 Bartels Stephen P Compositions and Methods for Effecting Controlled Posterior Vitreous Detachment
US20070212358A1 (en) * 2006-03-10 2007-09-13 Bartels Stephen P Compositions and Methods for Effecting Controlled Posterior Vitreous Detachment
BRPI0819780B1 (pt) 2007-11-29 2023-01-24 Grifols Therapeutics Inc Polinucleotídeo e método para produzir um ou mais polipeptídeos de plasmina recombinante
RU2495131C2 (ru) * 2008-02-19 2013-10-10 Байокон Лимитид Способ получения рекомбинантного инсулина гларгина
US8617863B2 (en) 2008-06-04 2013-12-31 Grifols Therapeutics Inc. Composition, method, and kit for preparing plasmin
PT2403865E (pt) 2009-03-03 2015-11-18 Grifols Therapeutics Inc Métodos de preparação de plasminogénio
RU2564131C2 (ru) 2009-07-10 2015-09-27 ТромбоДженикс НВ Варианты плазминогена и плазмина
US20120189609A1 (en) 2009-08-28 2012-07-26 Thrombogenics Nv Improvement to trabeculectomy
BR112013017172A2 (pt) 2011-01-05 2016-10-11 Thrombogenics Nv variantes de plasminogênio e plasmina
KR20140064841A (ko) 2011-08-12 2014-05-28 쓰롬보제닉스 엔.브이. 플라스미노겐 변이체 및 플라스민 변이체
CN110066783B (zh) * 2019-05-16 2021-07-13 重庆派金生物科技有限公司 一种无自切形式的微纤溶酶制备方法
CN113355309B (zh) * 2021-08-10 2021-11-05 迈威(上海)生物科技股份有限公司 重组截短型人纤维蛋白溶酶制备工艺

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4774087A (en) * 1987-08-14 1988-09-27 Northwestern University Micro-size fibrinolytic plasmin
JPH05500748A (ja) * 1989-05-01 1993-02-18 ザ　ユニバーシティ　オブ　ノートル　ダム　デュ　ラック 真核細胞系でのヒトプラスミノーゲンの発現方法および物質
GB9222758D0 (en) * 1992-10-29 1992-12-09 British Bio Technology Proteins and nucleic acids
US5288489A (en) * 1991-08-28 1994-02-22 Orion Therapeutic Systems, Inc. Fibrinolysis and fibrinogenolysis treatment
DE69934595T2 (de) * 1998-09-29 2007-10-04 Leuven Research & Development V.Z.W. Verwendung von verbindungen,die alpha2-antiplasmin in vivo reduzieren,zur herstellung einer zusammensetzung zur behandlung ischämischer schlaganfälle

Also Published As

Publication number Publication date
JP4250660B2 (ja) 2009-04-08
ES2296705T3 (es) 2008-05-01
JP2007195558A (ja) 2007-08-09
ATE378413T1 (de) 2007-11-15
DE60131450D1 (de) 2007-12-27
US7445775B2 (en) 2008-11-04
WO2002050290A1 (en) 2002-06-27
AU2002218890A1 (en) 2002-07-01
DE60131450T2 (de) 2008-10-16
JP2004526427A (ja) 2004-09-02
EP1343903B1 (en) 2007-11-14
JP4047170B2 (ja) 2008-02-13
US20040071676A1 (en) 2004-04-15
EP1343903A1 (en) 2003-09-17
CY1107866T1 (el) 2013-06-19
PT1343903E (pt) 2008-02-18

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