DK1077262T3 - Fremgangsmåde til fremstilling af naturligt foldede og secernerede proteiner ved co-sekretion af molekylære chaperoner - Google Patents

Fremgangsmåde til fremstilling af naturligt foldede og secernerede proteiner ved co-sekretion af molekylære chaperoner

Info

Publication number
DK1077262T3
DK1077262T3 DK00115839T DK00115839T DK1077262T3 DK 1077262 T3 DK1077262 T3 DK 1077262T3 DK 00115839 T DK00115839 T DK 00115839T DK 00115839 T DK00115839 T DK 00115839T DK 1077262 T3 DK1077262 T3 DK 1077262T3
Authority
DK
Denmark
Prior art keywords
secretion
preparation
periplasm
naturally folded
secreted proteins
Prior art date
Application number
DK00115839T
Other languages
English (en)
Inventor
Dorothee Ambrosius
Rainer Rudolph
Joerg Schaeffner
Elisabeth Schwarz
Original Assignee
Hoffmann La Roche
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Hoffmann La Roche filed Critical Hoffmann La Roche
Application granted granted Critical
Publication of DK1077262T3 publication Critical patent/DK1077262T3/da

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/6456—Plasminogen activators
    • C12N9/6459—Plasminogen activators t-plasminogen activator (3.4.21.68), i.e. tPA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C12Y304/21069—Protein C activated (3.4.21.69)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Enzymes And Modification Thereof (AREA)
DK00115839T 1999-07-29 2000-07-24 Fremgangsmåde til fremstilling af naturligt foldede og secernerede proteiner ved co-sekretion af molekylære chaperoner DK1077262T3 (da)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
EP99114811A EP1077263A1 (de) 1999-07-29 1999-07-29 Verfahren zur Herstellung von natürlich gefalteten und sekretierten Proteinen durch Co-Sekretion von Chaperonen

Publications (1)

Publication Number Publication Date
DK1077262T3 true DK1077262T3 (da) 2005-11-07

Family

ID=8238680

Family Applications (1)

Application Number Title Priority Date Filing Date
DK00115839T DK1077262T3 (da) 1999-07-29 2000-07-24 Fremgangsmåde til fremstilling af naturligt foldede og secernerede proteiner ved co-sekretion af molekylære chaperoner

Country Status (13)

Country Link
US (1) US6455279B1 (da)
EP (2) EP1077263A1 (da)
JP (1) JP2001061487A (da)
KR (1) KR100378325B1 (da)
CN (1) CN1231595C (da)
AT (1) ATE299942T1 (da)
CA (1) CA2313248C (da)
DE (1) DE60021318T2 (da)
DK (1) DK1077262T3 (da)
ES (1) ES2247990T3 (da)
IN (1) IN190327B (da)
JO (1) JO2309B1 (da)
MX (1) MXPA00007339A (da)

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US7087412B2 (en) 2000-11-14 2006-08-08 Boehringer Ingelheim International Gmbh Methods for large scale protein production in prokaryotes
US6955910B2 (en) 2000-11-14 2005-10-18 Boehringer Ingelheim International Gmbh Method for large scale production of recombinant DNA-derived TPA or K2S molecules
US6979556B2 (en) * 2000-12-14 2005-12-27 Genentech, Inc. Separate-cistron contructs for secretion of aglycosylated antibodies from prokaryotes
US7094884B2 (en) 2001-06-22 2006-08-22 Roche Diagnostics Corporation Soluble complexes of amylod β peptide and peptidyl prolyl isomerase chaperone and methods of making and using them
US6962982B2 (en) 2001-06-22 2005-11-08 Roche Diagnostics Corporation Soluble complexes of target proteins and peptidyl prolyl isomerase chaperones and methods of making and using them
EP2267452B8 (en) * 2001-06-22 2012-11-14 Roche Diagnostics GmbH A soluble complex comprising a retroviral surface glycoprotein
US7094757B2 (en) 2001-06-22 2006-08-22 Roche Diagnostics Corporation Complexes comprising a prion protein and a peptidyl prolyl isomerase chaperone, and method for producing and using them
KR100441755B1 (ko) * 2001-07-24 2004-08-02 재단법인서울대학교산학협력재단 분자 샤페론을 핵 내부로 이동시키는 방법
WO2003018771A2 (en) * 2001-08-27 2003-03-06 Genentech, Inc. A system for antibody expression and assembly
DE10145694A1 (de) * 2001-09-17 2003-04-03 Roche Diagnostics Gmbh Verfahren zur Erhöhung der Löslichkeit, der Expressionsrate und der Aktivität von Proteinen während der rekombinanten Herstellung
EP1578447A4 (en) * 2002-10-31 2009-06-03 Genentech Inc METHODS AND COMPOSITIONS THAT CAN INCREASE ANTIBODY PRODUCTION
EP1585768A2 (en) * 2003-01-23 2005-10-19 Genentech, Inc. Methods for producing humanized antibodies and improving yield of antibodies or antigen binding fragments in cell culture
WO2005004894A2 (en) * 2003-05-12 2005-01-20 Expressive Constructs, Inc. Methods for increasing cell and tissue viability
EP1663306A2 (en) * 2003-09-05 2006-06-07 Genentech, Inc. Antibodies with altered effector functions
GB0329681D0 (en) * 2003-12-23 2004-01-28 Delta Biotechnology Ltd Gene expression technique
US9057061B2 (en) 2003-12-23 2015-06-16 Novozymes Biopharma Dk A/S Gene expression technique
JP2008511337A (ja) * 2004-09-02 2008-04-17 ジェネンテック・インコーポレーテッド ヘテロ多量体分子
CN101180308A (zh) * 2005-05-23 2008-05-14 韩国科学技术院 利用sHSPs制备目的蛋白的方法
GB0512707D0 (en) * 2005-06-22 2005-07-27 Delta Biotechnology Ltd Gene expression technique
US8067179B2 (en) 2006-11-30 2011-11-29 Research Development Foundation Immunoglobulin libraries
CN101294164B (zh) * 2007-04-23 2013-01-16 南京大学 重组人中期因子(rh-Midkine)的表达及其单克隆抗体的制备和应用
US8629245B2 (en) * 2007-05-01 2014-01-14 Research Development Foundation Immunoglobulin Fc libraries
CN101307310B (zh) * 2007-05-15 2012-08-08 康泰生医科技股份有限公司 具有提高产率及免疫原性的重组蛋白质表达系统
JP5683581B2 (ja) * 2009-06-30 2015-03-11 リサーチ ディベロップメント ファウンデーション 免疫グロブリンＦｃポリペプチド
EP2673299B1 (en) 2011-02-07 2017-05-10 Research Development Foundation Engineered immunoglobulin fc polypeptides
WO2014054744A1 (ja) * 2012-10-03 2014-04-10 協和発酵キリン株式会社 培養液にアミノ酸を添加することによるポリペプチドの還元防止方法
WO2014089375A1 (en) 2012-12-05 2014-06-12 Boston Strategics Corporation Protein expression enhancing polypeptides
US10457737B2 (en) 2015-02-09 2019-10-29 Research Development Foundation Engineered immunoglobulin Fc polypeptides displaying improved complement activation
CN105296652B (zh) * 2015-11-26 2018-07-06 天津师范大学 采用荧光RT-PCR技术检测周氏啮小蜂Hsp40基因表达的方法
CN105296653B (zh) * 2015-11-26 2018-07-06 天津师范大学 采用荧光RT-PCR技术检测周氏啮小蜂Hsp83基因表达的方法
CN105296651B (zh) * 2015-11-26 2018-07-06 天津师范大学 采用荧光RT-PCR技术检测周氏啮小蜂Hsp90基因表达的方法
CN105296654B (zh) * 2015-11-26 2018-07-06 天津师范大学 采用荧光RT-PCR技术检测周氏啮小蜂Hsp60基因表达的方法
CN110734480B (zh) * 2019-11-07 2021-08-24 中国科学院遗传与发育生物学研究所 大肠杆菌分子伴侣GroEL/ES在协助合成植物Rubisco中的应用
US10973908B1 (en) 2020-05-14 2021-04-13 David Gordon Bermudes Expression of SARS-CoV-2 spike protein receptor binding domain in attenuated salmonella as a vaccine
CN112239760B (zh) * 2020-09-18 2022-02-11 深圳科兴药业有限公司 高效表达重组hGH的重组工程菌及构建方法和应用
CN112940955B (zh) * 2021-04-18 2022-10-18 江南大学 一种高效合成乳铁蛋白的毕赤酵母及其构建方法与应用

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Also Published As

Publication number Publication date
KR20010049895A (ko) 2001-06-15
IN190327B (da) 2003-07-19
CA2313248A1 (en) 2001-01-29
EP1077262A1 (en) 2001-02-21
EP1077262B1 (en) 2005-07-20
CN1283702A (zh) 2001-02-14
ATE299942T1 (de) 2005-08-15
DE60021318T2 (de) 2006-05-24
JO2309B1 (en) 2005-09-12
US6455279B1 (en) 2002-09-24
ES2247990T3 (es) 2006-03-16
DE60021318D1 (de) 2005-08-25
MXPA00007339A (es) 2002-08-06
CN1231595C (zh) 2005-12-14
EP1077263A1 (de) 2001-02-21
JP2001061487A (ja) 2001-03-13
CA2313248C (en) 2010-05-25
KR100378325B1 (ko) 2003-03-29

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