PT1343903E - ''um vector de expressão de levedura e um método de produção de uma proteína recombinante por expressão numa célula de levedura - Google Patents

''um vector de expressão de levedura e um método de produção de uma proteína recombinante por expressão numa célula de levedura Download PDF

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Publication number
PT1343903E
PT1343903E PT01271444T PT01271444T PT1343903E PT 1343903 E PT1343903 E PT 1343903E PT 01271444 T PT01271444 T PT 01271444T PT 01271444 T PT01271444 T PT 01271444T PT 1343903 E PT1343903 E PT 1343903E
Authority
PT
Portugal
Prior art keywords
expression
yeast
making
recombinant protein
expression vector
Prior art date
Application number
PT01271444T
Other languages
English (en)
Inventor
Desire Jose Collen
Nubuo Nagai
Yves Laroche
Original Assignee
Thrombogenics Nv
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from GB0031196A external-priority patent/GB0031196D0/en
Priority claimed from GB0116702A external-priority patent/GB0116702D0/en
Priority claimed from GB0116690A external-priority patent/GB0116690D0/en
Application filed by Thrombogenics Nv filed Critical Thrombogenics Nv
Publication of PT1343903E publication Critical patent/PT1343903E/pt

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/6435—Plasmin (3.4.21.7), i.e. fibrinolysin
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P7/00—Drugs for disorders of the blood or the extracellular fluid
    • A61P7/02—Antithrombotic agents; Anticoagulants; Platelet aggregation inhibitors
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P9/00—Drugs for disorders of the cardiovascular system
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P9/00—Drugs for disorders of the cardiovascular system
    • A61P9/10—Drugs for disorders of the cardiovascular system for treating ischaemic or atherosclerotic diseases, e.g. antianginal drugs, coronary vasodilators, drugs for myocardial infarction, retinopathy, cerebrovascula insufficiency, renal arteriosclerosis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C12Y304/21007—Plasmin (3.4.21.7), i.e. fibrinolysin

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Animal Behavior & Ethology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Heart & Thoracic Surgery (AREA)
  • Cardiology (AREA)
  • Microbiology (AREA)
  • Vascular Medicine (AREA)
  • Diabetes (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Peptides Or Proteins (AREA)
PT01271444T 2000-12-21 2001-12-20 ''um vector de expressão de levedura e um método de produção de uma proteína recombinante por expressão numa célula de levedura PT1343903E (pt)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GB0031196A GB0031196D0 (en) 2000-12-21 2000-12-21 Recombinant human plasminogen and plasmin: methods for high yield production stabilization and use for treatment
GB0116702A GB0116702D0 (en) 2001-07-09 2001-07-09 Human plasminogen and plasmin
GB0116690A GB0116690D0 (en) 2001-07-09 2001-07-09 Human plasminogen and plasmin

Publications (1)

Publication Number Publication Date
PT1343903E true PT1343903E (pt) 2008-02-18

Family

ID=27256020

Family Applications (1)

Application Number Title Priority Date Filing Date
PT01271444T PT1343903E (pt) 2000-12-21 2001-12-20 ''um vector de expressão de levedura e um método de produção de uma proteína recombinante por expressão numa célula de levedura

Country Status (11)

Country Link
US (1) US7445775B2 (pt)
EP (1) EP1343903B1 (pt)
JP (2) JP4047170B2 (pt)
AT (1) ATE378413T1 (pt)
AU (1) AU2002218890A1 (pt)
CY (1) CY1107866T1 (pt)
DE (1) DE60131450T2 (pt)
DK (1) DK1343903T3 (pt)
ES (1) ES2296705T3 (pt)
PT (1) PT1343903E (pt)
WO (1) WO2002050290A1 (pt)

Families Citing this family (22)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6998121B2 (en) * 2003-01-23 2006-02-14 Milkhaus Laboratory, Inc. Method of treatment of connective tissue disorders by administration of streptolysin O
DE10390418D2 (de) * 2002-02-06 2005-01-13 N Zyme Biotec Gmbh Verfahren zur Herstellung von rekombinanten Proteinen in Mikroorganismen
CA2475277A1 (en) * 2002-02-06 2003-08-14 Trommsdorff Gmbh & Co. Kg Arzneimittel Method for producing recombinant proteins in micro-organisms
GB0228409D0 (en) * 2002-12-06 2003-01-08 Thromb X Nv Pharmacological vitreolysis
KR100595864B1 (ko) * 2004-01-27 2006-06-30 재단법인 목암생명공학연구소 형질전환 사카로마이세스 세레비지애 균주 및 이를 이용한ｌｋ8 단백질의 생산방법
AU2005212431B2 (en) 2004-02-11 2010-07-15 Synthon Biopharmaceuticals B.V. Expression of plasminogen and microplasminogen in duckweed
US8034913B2 (en) 2004-04-22 2011-10-11 Grifols Therapeutics Inc. Recombinantly modified plasmin
CN100368524C (zh) * 2005-05-17 2008-02-13 上海大学 巴士德毕赤酵母gs115/pfk-k5的高密度发酵方法
US20070134230A1 (en) * 2005-12-13 2007-06-14 Jani Dharmendra M Method for prolonging activity of autodegradable enzymes
US20070134231A1 (en) * 2005-12-13 2007-06-14 Jani Dharmendra M Method for prolonging activity of autodegradable enzymes and compositions thereof
US20070196350A1 (en) * 2006-02-22 2007-08-23 Bartels Stephen P Compositions and Methods for Effecting Controlled Posterior Vitreous Detachment
US20070212358A1 (en) * 2006-03-10 2007-09-13 Bartels Stephen P Compositions and Methods for Effecting Controlled Posterior Vitreous Detachment
NZ585715A (en) * 2007-11-29 2011-11-25 Talecris Biotherapeutics Inc Recombinantly modified plasmin
JP5781308B2 (ja) * 2008-02-19 2015-09-16 バイオコン・リミテッドＢｉｏｃｏｎ Ｌｉｍｉｔｅｄ 異種タンパク質を得る方法およびインスリン類似体
AU2009256168B2 (en) 2008-06-04 2014-06-05 Grifols Therapeutics Inc. Composition, method and kit for preparing plasmin
US9206410B2 (en) 2009-03-03 2015-12-08 Grifols Therapeutics Inc. Compositions, methods and kits for preparing plasminogen and plasmin prepared therefrom
KR20120050442A (ko) * 2009-07-10 2012-05-18 쓰롬보제닉스 엔.브이. 플라스미노겐 및 플라스민의 변이체
EP2480249B1 (en) 2009-08-28 2015-01-28 ThromboGenics N.V. Plasmin for the treatment of filtration failure after trabeculectomy
JP6085568B2 (ja) 2011-01-05 2017-02-22 スロンボジェニックス・ナムローゼ・フェンノートシャップＴｈｒｏｍｂｏＧｅｎｉｃｓ ＮＶ プラスミノーゲンおよびプラスミンの変異体
AU2012296884B2 (en) 2011-08-12 2015-02-05 Thrombogenics N.V. Plasminogen and plasmin variants
CN110066783B (zh) * 2019-05-16 2021-07-13 重庆派金生物科技有限公司 一种无自切形式的微纤溶酶制备方法
CN113355309B (zh) * 2021-08-10 2021-11-05 迈威(上海)生物科技股份有限公司 重组截短型人纤维蛋白溶酶制备工艺

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4774087A (en) 1987-08-14 1988-09-27 Northwestern University Micro-size fibrinolytic plasmin
JPH05500748A (ja) * 1989-05-01 1993-02-18 ザ　ユニバーシティ　オブ　ノートル　ダム　デュ　ラック 真核細胞系でのヒトプラスミノーゲンの発現方法および物質
GB9222758D0 (en) 1992-10-29 1992-12-09 British Bio Technology Proteins and nucleic acids
US5288489A (en) 1991-08-28 1994-02-22 Orion Therapeutic Systems, Inc. Fibrinolysis and fibrinogenolysis treatment
EP1117437B1 (en) 1998-09-29 2006-12-27 Leuven Research & Development vzw Use of compounds that reduce alpha2-antiplasmin in vivo for the preparation of a composition for the treatment of ischemic stroke

Also Published As

Publication number Publication date
DE60131450D1 (de) 2007-12-27
CY1107866T1 (el) 2013-06-19
EP1343903A1 (en) 2003-09-17
JP4047170B2 (ja) 2008-02-13
US20040071676A1 (en) 2004-04-15
DE60131450T2 (de) 2008-10-16
JP2007195558A (ja) 2007-08-09
AU2002218890A1 (en) 2002-07-01
WO2002050290A1 (en) 2002-06-27
ATE378413T1 (de) 2007-11-15
EP1343903B1 (en) 2007-11-14
ES2296705T3 (es) 2008-05-01
JP4250660B2 (ja) 2009-04-08
JP2004526427A (ja) 2004-09-02
US7445775B2 (en) 2008-11-04
DK1343903T3 (da) 2008-03-10

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