TWI734331B - 鳳梨萃取物的護膚用途 - Google Patents
鳳梨萃取物的護膚用途 Download PDFInfo
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- TWI734331B TWI734331B TW108148714A TW108148714A TWI734331B TW I734331 B TWI734331 B TW I734331B TW 108148714 A TW108148714 A TW 108148714A TW 108148714 A TW108148714 A TW 108148714A TW I734331 B TWI734331 B TW I734331B
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- Prior art keywords
- skin
- cells
- pineapple
- pineapple peel
- peel extract
- Prior art date
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Abstract
本發明提供一種鳳梨萃取物用於製備在皮膚抑制黑色素生成、增加含水量、提升光澤、及減少毛孔之醫藥組合物之用途,亦提供一種鳳梨萃取物用於製備提升細胞抗氧化力之醫藥組合物之用途。
Description
本發明係關於一種鳳梨萃取物的用途,特別係關於一種鳳梨果皮萃取物用於製備在皮膚抑制黑色素生成、增加含水量、提升光澤、及減少毛孔之醫藥組合物之用途,及一種鳳梨果皮萃取物用於製備提升細胞抗氧化力之醫藥組合物之用途。
皮膚是人體隔絕外界環境中的刺激,例如紫外線、病原體、摩擦力等,與防止水分流失的首要防線。皮膚具有表皮層、真皮層、及皮下組織之三層結構。表皮是皮膚的最外層並且不斷更新。表皮與真皮間存在持續分裂的細胞,例如纖維母細胞、角質細胞、黑色素細胞。真皮層富含膠原蛋白、彈性蛋白、及吸水力強的玻尿酸,其賦予肌膚彈性和支撐力量。由於年齡增長及環境刺激,皮膚會出現斑點、膚色黯沉、皺紋、鬆弛、凹陷、及毛孔粗大等老化外觀,並且累積氧化傷害。為了延緩皮膚老化,抑制黑色素過度生成、增加皮膚含水量及提升皮膚抗氧化力極為重要。
習知用以改善皮膚老化外觀的方法包括對皮膚施用抑制黑色素生成的化合物,及注射膠原蛋白或玻尿酸至真皮層。然而,部分抑制黑色素生成的化合物可能導致皮膚過敏,例如麴酸(kojic acid)。此外,注射至皮膚的膠原蛋白或玻尿酸易隨時間被體內酵素分解,導致必須定期施打該些物質,成本高昂。
此外,習知提升皮膚抗氧化力的方法主要是直接使用抗氧化劑於皮膚。該些抗氧化劑雖能協助清除造成皮膚細胞損傷的自由基,但無法透過改變細胞生理條件而提升皮膚細胞本身的抗氧化力。
有鑑於此,開發一種兼具皮膚美白、增加皮膚含水量、及提升細胞抗氧化力的組合物以製備新型態的護膚產品,實有其必要。
本發明之一目的在提供一種鳳梨萃取物用於製備在皮膚抑制黑色素生成、增加含水量、提升光澤、及減少毛孔之醫藥組合物之用途,其中該鳳梨萃取物係以一溶劑萃取一鳳梨果皮而獲得。
在本發明之一實施例中,該溶劑與該鳳梨果皮之重量比範圍為20:1至1:1,較佳為10:1至1:1。
在本發明之一實施例中,該溶劑為水,且該萃取係在50℃至100℃進行,較佳為65℃至85℃。
本發明之另一目的在提供一種前述鳳梨萃取物用於製備提升細胞抗氧化力之醫藥組合物之用途。
在本發明之一實施例中,該鳳梨萃取物增加該細胞內穀胱甘肽(glutathione,簡稱GSH)的含量,或增加該細胞之穀胱甘肽S轉移酶(glutathione S-transferase,簡稱GST)活性。
本發明揭露鳳梨萃取物具有抑制黑色素生成及改善膚質的功效,包括提升皮膚含水量與光澤及減少毛孔。該鳳梨萃取物亦能提升包含皮膚細胞之多種細胞的抗氧化力,藉此減少個體或皮膚的氧化傷害。因此,該鳳梨萃取物可用於製備在皮膚抑制黑色素生成、增加含水量、提升光澤、及減少毛孔之組合物,或製備提升細胞抗氧化力之組合物。該組合物可具有粉末、顆粒、溶液、膠體或膏體之形式,且可製成醫藥品、食品、飲品、或營養補充劑,藉由口服、局部施用於皮膚或其他方式給予一個體。
以下將配合圖式進一步說明本發明的實施方式,下述所列舉的實施例係用以闡明本發明之特點及應用,而非用以限定本發明之範圍,任何熟習此技藝者,在不脫離本發明之精神和範圍內,當可做些許更動與潤飾,因此本發明之保護範圍當視後附之申請專利範圍所界定者為準。
圖1顯示皮膚纖維母細胞以本發明一實施例之鳳梨果皮萃取物處理後的穀胱甘肽相對含量。
圖2顯示肝癌細胞株以本發明一實施例之鳳梨果皮萃取物處理後的穀胱甘肽相對含量。
圖3顯示皮膚纖維母細胞以本發明一實施例之鳳梨果皮萃取物處理後的穀胱甘肽S轉移酶活性。
圖4顯示外周血單核細胞以本發明一實施例之鳳梨果皮萃取物處理後的穀胱甘肽S轉移酶活性。
圖5顯示本發明一實施例之鳳梨果皮萃取物對過氧化氫(H2O2)刺激下皮膚纖維母細胞活性氧物質生成的抑制作用。
圖6顯示黑色素瘤細胞以本發明一實施例之鳳梨果皮萃取物處理後的黑色素相對含量。
圖7顯示受試者施用一含1%鳳梨果皮萃取物之面膜組合物或一基底精華液之前或施用15分鐘後的皮膚含水量。
圖8顯示受試者施用一含1%鳳梨果皮萃取物之面膜組合物或一基底精華液之前或施用15分鐘後的皮膚光澤度。
圖9顯示受試者施用一含1%鳳梨果皮萃取物之面膜組合物或一基底精華液之前或15分鐘後的皮膚毛孔數量。
除非另有說明,本文中所使用之「一」、「該」及類似用語應理解為包含單數及複數形式。
本文中所使用數值為近似值,所有實驗數據皆表示在20%的範圍內,較佳為在10%的範圍內,最佳為在5%的範圍內。
本文所述的醫藥組合物可利用熟習此技藝者所詳知的技術而製備成一適合於非經腸道地(parenterally)或口服地(orally)施用的劑型(dosage form),其包括但不限於:注射品(injection)[例如,無菌的水性溶液(sterile aqueous solution)或分散液(dispersion)]、粉末(sterile powder)、錠劑(tablet)、片劑(troche)、口含錠(lozenge)、丸劑(pill)、膠囊(capsule)、分散性粉末(dispersible powder)、細
顆粒(granule)、溶液、懸浮液(suspension)、乳劑(emulsion)、糖漿(syrup)、酏劑(elixir)、濃漿(slurry)以及類似之物。
本文所述的醫藥組合物可由非經腸道途徑(parenteral routes)施用,其包括但不限於:透皮(transdermal)施用、腹膜內注射(intraperitoneal injection)、皮下注射(subcutaneous injection)、肌肉內注射(intramuscular injection)、及靜脈內注射(intravenous injection)。
本文所述的醫藥組合物可包含一廣泛使用於藥物製造技術之醫藥上可接受的載劑(pharmaceutically acceptable carrier)。該醫藥上可接受的載劑可包含一或多種選自於由下列所構成之群組中的試劑:溶劑(solvent)、乳化劑(emulsifier)、懸浮劑(suspending agent)、分解劑(decomposer)、黏結劑(binding agent)、賦形劑(excipient)、安定劑(stabilizing agent)、螯合劑(chelating agent)、稀釋劑(diluent)、膠凝劑(gelling agent)、防腐劑(preservative)、潤滑劑(lubricant)、吸收延遲劑(absorption delaying agent)、脂質體(liposome)以及類似之物。該些試劑的選用與數量落在熟習此項技術者的專業素養與例行技術範疇內。
前述醫藥上可接受的載劑包含有一選自於由下列所構成之群組中的溶劑:水、生理鹽水(normal saline)、磷酸鹽緩衝鹽溶液(phosphate buffered saline,PBS)、含糖溶液、含醇水溶液(aqueous solution containing alcohol)、及它們的組合。
自Thermo Fisher Scientific公司購買含Earle’s平衡鹽溶液之Eagle’s最低基本培養基(Gibco Eagle’s minimum essential medium,MEM)、DMEM培養基(Gibco Dulbecco’s modified Eagle’s medium)、胎牛血清(Gibco fetal bovine serum,FBS)、青黴素/鏈黴素(Gibco penicillin/streptomycin)、非必需胺基酸、碳酸氫鈉、丙酮酸鈉、及磷酸緩衝鹽溶液(Gibco phosphate buffered saline,PBS)。自PEPROTECH公司購買人類巨噬細胞聚落刺激因子(macrophage colony stimulating factor,M-CSF)及人細胞核因子KB受體活化因子配體(receptor activator of nuclear factor kappaB ligand,RANKL)。自abcam公司購買穀胱甘肽檢測試劑組(GSH Assay Kit ab112132)及穀胱甘肽S轉移酶活性檢測試劑組(GST
Activity Assay Kit ab65326)。自ECHO Chemical公司購買二甲基亞碸(dimethyl sulfoxide,DMSO)。自Sigma公司購買過氧化氫(H2O2)及二氯二氫螢光素二乙酸酯(2,7-dichloro-dihydro-fluorescein diacetate,DCFH-DA)。該DCFH-DA使用前係溶於DMSO以配製為5mg/mL DCFH-DA溶液。
以下實施例所用細胞包括購自食品工業發展研究所生物資源保存及研究中心(Bioresource Collection and Research Center,BCRC)之人類皮膚纖維母細胞CCD-966SK(ATCC BCRC 60153),以及購自美國典型培養物保存中心(American Type Culture Collection,ATCC)之小鼠黑色素瘤細胞株B16F10(ATCC CRL-6475)、人類肝癌細胞株HepG2(ATCC HB-8065)、及人類外周血單核細胞(peripheral mononuclear mononuclear cell,PBMC)。CCD-966SK細胞在37℃、5%二氧化碳的條件下培養於添加10% FBS、1mM丙酮酸鈉、1.5g/L碳酸氫鈉、及0.1mM非必需胺基酸之MEM培養基,以下稱MEM細胞培養基。PBMC細胞在37℃、5%二氧化碳的條件下培養於添加10% FBS、40ng/mL RANKL、25ng/mLM-CSF、1%青黴素/鏈黴素的MEM培養基,以下稱蝕骨細胞分化培養基。B16F10細胞及HepG2細胞係在37℃、5%二氧化碳的條件下培養於添加10% FBS及1%青黴素/鏈黴素的DMEM培養基,以下稱DMEM細胞培養基。
細胞內GSH含量之測定係使用abcam之GSH檢測試劑組。簡言之,依據廠商使用說明,以PBS溶液清洗經過指定處理的細胞及再懸浮該細胞於1mL PBS溶液。其後,以巰基染劑(稀釋1000倍)在37℃對該細胞染色15分鐘。該染劑本是非螢光化合物,但與巰基反應後會發出綠色螢光。染色後,該細胞以PBS溶液清洗及再懸浮於200μL PBS溶液,再以流式細胞儀(flow cytometry;BD)偵測綠色螢光訊號(激發波長為490nm,偵測波長為520nm)。
細胞內GST活性之測定係使用abcam之GST活性檢測試劑組。簡言之,依據廠商使用說明,以PBS溶液清洗經過指定處理的細胞及再懸浮該細胞於150μL GST檢測緩衝液(GST assay buffer),藉由吸放(pipetting)該懸浮液數次以獲得一細胞裂解液。其後,以離心方式(10000g,4℃,15分鐘)收集該細胞裂解
液的上清液。將50μL/孔之該上清液(實驗組)、正控制組試劑、或GST檢測緩衝液加入一96孔盤,並於各孔添加5μL GSH及50μL反應液(含1μL GST受質溶液及49μL GST檢測緩衝液),所得混合液於室溫反應並且每隔3分鐘測量在340nm的吸光值(OD340),持續15分鐘。最終,計算每分鐘的OD340變化及進一步計算GST活性。
黑色素瘤細胞株B16F10的黑色素含量測定方法簡述如下。自經過指定處理的細胞培養物中收集細胞。該細胞以PBS溶液清洗並以胰蛋白酶(trypsin)溶液處理3分鐘,所得懸浮細胞以離心方式(400 xg,5分鐘)收集,經PBS溶液清洗二次,而後再懸浮於200μLPBS溶液。該細胞懸浮液以液態氮冷凍10分鐘,再置於室溫約30分鐘至完全解凍後,以離心方式(12,000 xg,3分鐘)移除上清液。餘下細胞沉澱與120μL之1N氫氧化鈉水溶液混合均勻,再於60℃加熱1小時以獲得一含溶解黑色素的細胞裂解液。將100μL該細胞裂解液移入一96孔盤,並使用ELISA讀盤機(enzyme-linked immunosorbent assay reader;BioTek)測量該細胞裂解液在450nm的吸光值(OD 450)。黑色素相對含量係依下列公式計算:
黑色素相對含量(%)=(各組OD450值/對照組OD450值)×100%。
皮膚含水量係利用多功能皮膚檢測系統MPA 580(C+K electronic,德國)之含水量測定探頭(Corneometer® CM825),藉由導電性變化測量待測區域的皮膚含水量。測試過程中,以Corneometer探頭按壓受試者的皮膚待測區域(避免同一點連續測量),進而得到皮膚含水量數值。由於環境的溫度及濕度會影響皮膚含水量,受試者需要先進入恆溫、恆濕的檢測室平衡20分鐘後才進行測試。測試之環境條件為溫度20℃±1℃,相對溼度50%±10%,且避免空氣擾動。
皮膚光澤度係利用多功能皮膚檢測系統MPA 580(C+K electronic,德國)之光澤度測定探頭(Glossymeter GL 200),藉由照射到皮膚表面光線的直接反射和散射而得到皮膚光澤度數值。
皮膚毛孔數量係利用VISIA-CR全臉膚質檢測儀(Canfield scientific,USA)之全光域波長及偏光波長的光源,於密閉的照相室內拍攝受試者的臉部,取得清晰圖檔後,再以影像軟體分析而得。
數據表示為平均值±標準差(SD)。使用Excel軟體進行統計分析,數據間在統計上的顯著差異以學生t檢驗(student's t-test)判定。
本文所述的鳳梨(Ananas comosus)果皮係指成熟鳳梨果實的表面覆蓋層。鳳梨是一種熱帶水果,其果實是複數鳳梨花的果實結合而成的單一複合果(multiple fruit)。未成熟的鳳梨果實呈綠色,其在成熟後轉為黃色。
為獲得一鳳梨果皮萃取物,自一鳳梨果實取得果皮,並將該鳳梨果皮洗淨及破碎成小塊。其後,以水、醇類、或醇水混合物為溶劑對鳳梨果皮碎塊進行萃取。醇類的例子包括甲醇、乙醇、正丙醇、異丙醇等低碳數的醇類。該溶劑與該鳳梨果皮之重量比為20:1至1:1,較佳為10:1至5:1。萃取溫度為介於50℃至100℃,較佳為75℃至95℃。以下實施例2-6所述鳳梨果皮萃取物皆係依前述方法以水萃取鳳梨果皮0.5至3小時而製得。
經上述萃取步驟所得鳳梨果皮萃取物冷卻至室溫後,可進一步使用200至400目(mesh)之濾網過濾,以移除殘餘固體物。該過濾後的鳳梨果皮萃取物可進一步在45℃至70℃進行減壓濃縮而獲得一濃縮產物。為獲得固態的鳳梨果皮萃取物,可將前述濃縮的鳳梨果皮萃取物以例如冷凍乾燥、噴霧乾燥等乾燥方式去除溶劑,因此獲得乾燥的鳳梨果皮萃取物。
為檢視鳳梨果皮萃取物對細胞內具抗氧化功效之穀胱甘肽(GSH)含量的影響,測定人類皮膚纖維母細胞CCD-966SK或人類肝癌細胞株HepG2以實施例1所述鳳梨果皮萃取物處理後的GSH含量變化。簡言之,將CCD-966SK細胞或HepG2細胞依2×105細胞/孔接種於6孔培養盤,各孔含有2mL MEM細胞培養基。在37℃隔夜培養細胞後,移除該培養基,再以含有0.3125
mg/mL鳳梨果皮萃取物之2mL MEM細胞培養基處理各孔細胞(實驗組)。另設置對照組,係僅以2mL MEM細胞培養基處理CCD-966SK細胞或HepG2細胞。在37℃培養24小時後,收集前述各組細胞以巰基螢光染劑測定GSH含量。
圖1顯示皮膚纖維母細胞經不同處理後的GSH相對含量,其數值表示為相對於對照組的螢光訊號強度的倍數;圖中***表示相比對照組為p<0.001。依據圖1,相比對照組,鳳梨果皮萃取物之處理顯著提升皮膚纖維母細胞的GSH含量至約1.9倍,說明施用鳳梨果皮萃取物能促進皮膚細胞內穀胱甘肽的生成,因此增加皮膚對氧化傷害的抵抗力,例如對紫外線傷害的抵抗力。
圖2顯示HepG2細胞經不同處理後的GSH相對含量;圖中*表示相比對照組為p<0.05。依據該圖,相比對照組,鳳梨果皮萃取物之處理明顯提升HepG2細胞的GSH含量至約1.4倍,說明施用鳳梨果皮萃取物能提高肝細胞內穀胱甘肽的生成。
為檢視鳳梨果皮萃取物對細胞內負責清除有毒物質(如氧化壓力所產生的活性氧物質)之穀胱甘肽S轉移酶(GST)活性的影響,評估人類皮膚纖維母細胞CCD-966SK或外周血單核細胞(PBMC)以實施例1所述鳳梨果皮萃取物處理後,其GST的總體活性變化。簡言之,將CCD-966SK細胞或PBMC細胞依2×105細胞/孔接種於6孔培養盤,各孔含有2mL MEM細胞培養基。在37℃隔夜培養細胞後,移除該培養基,並以含有0.3125mg/mL或0.625mg/mL鳳梨果皮萃取物之2mL MEM細胞培養基處理各孔細胞(實驗組)。另設置對照組,係僅以3mL MEM細胞培養基處理CCD-966SK細胞或PBMC細胞。在37℃培養24小時後,收集前述各組細胞用於GST活性分析。
圖3顯示皮膚纖維母細胞經不同處理後的GST相對活性,其數值表示為相對於對照組的GST活性的倍數;圖中*表示相比對照組為p<0.05。依據圖3,相比對照組,鳳梨果皮萃取物之處理顯著提升皮膚纖維母細胞的GST活性至約1.06倍,說明施用鳳梨果皮萃取物能增加皮膚細胞清除例如活性氧物質(ROS)之自由基的能力,因此有利於減少皮膚的氧化傷害,包括對去氧核醣核酸(DNA)、蛋白質及脂質等細胞組成分子的損傷。
圖4顯示PBMC細胞經不同處理後的GST相對活性;圖中***表示相比對照組為p<0.001。依據該圖,相比對照組,鳳梨果皮萃取物之處理顯著提升PBMC細胞的GST活性至約1.48倍,說明施用鳳梨果皮萃取物能增加體細胞的抗氧化力。
為驗證鳳梨果皮萃取物對皮膚細胞中氧化壓力的調節作用,利用螢光探針DCFH-DA配合流式細胞儀(flow cytometry;Beckman),測定人類皮膚纖維母細胞CCD-966SK以實施例1所述鳳梨果皮萃取物處理後,其在氧化刺激下的活性氧物質含量變化。簡言之,將2×105個CCD-966SK細胞接種於6孔培養盤,各孔含有2mL MEM細胞培養基。在37℃培養細胞24小時後,移除該培養基,並於37℃以2mL含有或不含1mg/mL鳳梨果皮萃取物之MEM細胞培養基處理細胞1小時,再以5μg/mL DCFH-DA溶液處理細胞15分鐘。其後,於37℃以有無過氧化氫的下列方式處理各孔細胞:(a)未經鳳梨果皮萃取物處理的細胞在無過氧化氫刺激下培養1小時(空白對照組);(b)未經鳳梨果皮萃取物處理的細胞以1mM過氧化氫處理1小時(負控制組);或(c)經鳳梨果皮萃取物處理的細胞以1mM過氧化氫處理1小時(實驗組)。其後,各組細胞以PBS溶液清洗二次,於暗處以200μL胰蛋白酶處理5分鐘,再藉由離心(400 xg,10分鐘)收集得各別細胞沉澱物。該細胞沉澱物以PBS溶液清洗一次及再次懸浮,並使用流式細胞儀偵測細胞的螢光強度(二重複試驗)。進行螢光偵測之激發波長為450-490nm,偵測波長為510-550nm。由於DCFH-DA進入細胞後會先被水解為DCFH(二氯二氫螢光素),再被活性氧物質氧化為可發出綠色螢光的DCF(二氯螢光素),經DCFH-DA處理之細胞的螢光強度可反映細胞內活性氧物質含量。
圖5顯示皮膚纖維母細胞經不同處理後的活性氧物質相對含量(%),以負控制組細胞的活性氧物質含量界定為100%;圖中***表示相比負控制組為p<0.001。依據圖5,負控制組相比空白對照組呈現明顯較高的活性氧物質含量,顯示過氧化氫刺激會導致細胞內活性氧物質之累積。然而,相比負控制組,鳳梨果皮萃取物之處理使細胞內活性氧物質降低約45%,顯示鳳梨果皮萃取物本身能清除自由基或提高個體細胞清除自由基的能力。
為檢視鳳梨果皮萃取物對黑色素生成的影響,測定黑色素瘤細胞株B16F10以實施例1所述鳳梨果皮萃取物處理後的黑色素含量變化。簡言之,將B16F10細胞依1.5×105細胞/孔接種於6孔培養盤,各孔含有3mL DMEM細胞培養基。在37℃培養細胞24小時後,移除該培養基,並以含有0.625mg/mL鳳梨果皮萃取物之3mL DMEM細胞培養基處理各孔細胞(實驗組)。另設置一對照組,係僅以3mL DMEM細胞培養基處理細胞。在37℃下培養48小時後,收集前述各組細胞以測定黑色素含量(三重複試驗)。
圖6顯示黑色素瘤細胞經不同處理後的黑色素相對含量,其數值表示為相對於對照組的黑色素含量的百分比;圖中**表示相比對照組為p<0.01。依據該圖,相比對照組,鳳梨果皮萃取物之處理使黑色含量顯著降低約15%,說明鳳梨果皮萃取物具有抑制黑色素生成的效果。
為評估鳳梨果皮萃取物對皮膚含水量、光澤、及毛孔的影響,六位25至40歲之受試者,於檢測前先清潔臉部,並待肌膚平衡穩定後,在左、右半臉各別施用添加一基底精華液之面膜,或添加1%(w/w)實施例1所述鳳梨果皮萃取物及該基底精華液之面膜,其中該基底精華液包含水、馨鮮酮、己二醇、1,3丁二醇、三仙膠、增稠劑、及三乙醇胺。於施用15分鐘後卸下面膜,以按摩方式促進有效成分吸收,再檢測施用前後受試者皮膚的含水量、光澤及毛孔數量。
圖7顯示受試者的平均皮膚含水量變化;圖8顯示受試者的平均皮膚光澤度變化;圖9顯示受試者皮膚的平均毛孔數量變化;圖中百分比各別表示相對於施用前的皮膚含水量、皮膚光澤度及毛孔數量;圖中**表示相比於施用面膜前的數據為p<0.01。依據圖7-9,相比施用前,施用鳳梨果皮萃取物面膜能顯著增加皮膚含水量約20.3%,顯著提升皮膚光澤度約13.1%,及有效減少皮膚毛孔數量約10.3%。該些結果說明該添加鳳梨果皮萃取物之面膜可改善膚質,包括提升皮膚含水量與光澤及減少毛孔。
綜上所述,本發明揭露鳳梨果皮萃取物具有抑制黑色素生成及改善膚質的功效,包括提升皮膚含水量與光澤及減少毛孔。該鳳梨果皮萃取物亦能提升包含皮膚細胞之多種細胞的抗氧化力,藉此減少個體或皮膚的氧化傷害。因此,該鳳梨果皮萃取物可用於製備在皮膚抑制黑色素生成、增加含水量、提升光澤、及減少毛孔之組合物,或製備提升細胞抗氧化力之組合物。該組合物可具有粉末、顆粒、溶液、膠體或膏體之形式,且可製成醫藥品、食品、飲品、或營養補充劑,藉由口服、局部施用於皮膚或其他方式給予一個體。
Claims (7)
- 一種鳳梨萃取物用於製備提升皮膚光澤及減少毛孔之口服地施用或於該皮膚上局部施用的組合物之用途,其中該鳳梨萃取物係以一溶劑萃取一鳳梨果皮而獲得,且該溶劑為水。
- 如申請專利範圍第1項所述之用途,其中該溶劑與該鳳梨果皮之重量比範圍為20:1至1:1。
- 如申請專利範圍第1項所述之用途,其中該萃取係在50℃至100℃進行。
- 一種鳳梨萃取物用於製備提升皮膚細胞抗氧化力之口服地施用或於該皮膚上局部施用的組合物之用途,其中該鳳梨萃取物係以一溶劑萃取一鳳梨果皮而獲得,且該溶劑為水,其中該鳳梨萃取物增加該皮膚細胞內穀胱甘肽的含量至1.9倍,且該鳳梨萃取物的有效量為0.3125mg/mL以上。
- 一種鳳梨萃取物用於製備提升皮膚細胞抗氧化力之口服地施用或於該皮膚上局部施用的組合物之用途,其中該鳳梨萃取物係以一溶劑萃取一鳳梨果皮而獲得,且該溶劑為水,其中該鳳梨萃取物增加該皮膚細胞之穀胱甘肽S轉移酶活性至1.06倍,且該鳳梨萃取物的有效量為0.625mg/mL以上。
- 如申請專利範圍第4或5項所述之用途,其中該溶劑與該鳳梨果皮之重量比範圍為20:1至1:1。
- 如申請專利範圍第4或5項所述之用途,其中該萃取係在50℃至100℃進行。
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