TW201225989A

TW201225989A - Sugar-free pineapple extract, production method thereof, and application thereof

Info

Publication number: TW201225989A
Application number: TW100139351A
Authority: TW
Inventors: Jun Nojima; Keiko Yashiki; Nobuaki Ohto; Yanyang Zhou; Akinori Kiso; Hiroshige Kuwahara; Ryoji Takagaki
Original assignee: Maruzen Pharm Co Ltd
Priority date: 2010-10-29
Filing date: 2011-10-28
Publication date: 2012-07-01
Also published as: KR102239121B1; JP2012097008A; JP5714869B2; KR20180037163A; KR20120046064A; TWI500428B; KR20160008128A

Abstract

The present invention relates to a sugar-free pineapple extract free from glucose and fructose obtained by extracting the edible part of a pineapple with a solvent, which has excellent safety and productivity, is cheap, and can be taken daily. The sugar-free pineapple extract has excellent production enhancing function for laminin-5 and hyaluronic acid, activity inhibiting function for hyaluronidase, etc..

Description

201225989 六、發明說明：【發明所屬之技術領域】本發明係關於不含有糖之鳳梨（pineapple )萃取物、及其製造方法、以及層黏連蛋白-5 (laminin-5)產生促進劑、表皮透明質酸（hyaluronic acid)產生促進劑、透明質酸酶（hyaluronidase )活性抑制劑、麩醯胺轉移峰^ (transglutaminase-1)產生促進劑、黑色素（melanin)產生抑制劑、毛乳頭細胞增殖促進劑、飲食品、皮膚外用劑、及毛髮生長劑.。【先前技術】皮膚係由表皮、基底膜、及真皮所構成。真皮係由纖維芽細胞、由該細胞分泌的膠原纖維（c〇Uagen)、彈性蛋白（elaStin)(彈性纖維）、透明質酸等之細胞外基質所構成。基底膜係存於表皮及真皮之交界部，不僅維繫表皮及真皮，而且於皮膚機能之維持上負有重要任務（參照非專利文獻1)。於年輕的皮膚，藉由保持此等皮膚組織之相互作用的恒m確保水分保持力、柔軟性、彈力性等外觀上亦為緊緻及潤澤而維持著嬌嫩的狀離。然而’受到紫外線、t苜益 -、頁者的空軋乾燥、過度之皮膚块淨、壓力、抽煙等的外在因子的影響，隨著老化的進行時為細胞外基質之主要構成成分的膠原纖維之分解或變質看發生、纖維芽細胞之增殖變々甘广时 i的、、或基底膜之主要構成成分的層黏連蛋白-5產生八初七^ 生刀解或變質而基底膜構造被破場201225989 VI. Description of the Invention: [Technical Field of the Invention] The present invention relates to a pineapple extract containing no sugar, a method for producing the same, and a laminin-5 production promoter and epidermis. Hyaluronic acid production promoter, hyaluronidase activity inhibitor, transglutaminase-1 production promoter, melanin production inhibitor, dermal papilla cell proliferation promotion Agent, food and drink, skin external preparation, and hair growth agent. [Prior Art] The skin is composed of the epidermis, the basement membrane, and the dermis. The dermis is composed of fibroblasts, collagen fibers (c〇Uagen) secreted by the cells, elastin (elastomeric fibers), and extracellular matrices such as hyaluronic acid. The basement membrane is deposited at the junction of the epidermis and the dermis, and not only maintains the epidermis and the dermis, but also plays an important role in maintaining the skin function (see Non-Patent Document 1). In young skin, by maintaining the mutual interaction of these skin tissues, it is ensured that the moisture retention, softness, elasticity, and the like are also firm and moist, and maintain a delicate appearance. However, it is affected by the external factors such as ultraviolet rays, t-benefits, empty rolling and drying of the pager, excessive skin mass, pressure, smoking, etc., and collagen which is the main component of the extracellular matrix as the aging progresses. When the decomposition or deterioration of the fiber occurs, the proliferation of the fibroblasts becomes a major component of the basement membrane, or the laminin-5, which is the main constituent of the basement membrane, produces a knife or a metamorphism and the basement membrane structure is broken. field

S 3 201225989 (參照非專利文獻2)。又，會引起為天然保濕因子的透明質酸之產生量的降低。此結果會成為呈現如皮膚之保濕機能或彈性降低、發生角質之異常剝離、皺紋、黯淡、質感消失、彈力性降低等之老化症狀。因此，一般認為經由促進層黏連蛋白-5之產生及透明質酸之產生，可防止甚至改善皮膚之老化。因此，正冀望自安全性高的天然物獲得具有層黏連蛋白-5產生促進作用及透明質酸產生促進作用的物質。迄今，就具有層黏連蛋白-5產生促進作用者而言，例如，已報告葛根（Puerariae radix)萃取物、甘草（licorice) 萃取物或其類黃酮（flavonoid )鶴份、檜扇（Iris domestica) 萃取物、黑板樹（Alstoniascholaris)萃取物、心葉青牛膽 (Tinosporacordifolia)萃取物、葫蘆巴（fenugreek)萃取物、乳清萃取物（以上，參照專利文獻1 )、紅芒柄花(Ononis spinosa)萃取物、黃苜藉（Medicagofalcata)萃取物、豆芽 %取物、紅豆（Vigna angularis )萃取物（以上，參照專利文獻2)、大豆萃取物（參照專利文獻3)等。又，正嘗試自天然物萃取具有透明質酸產生促進作用的物質（參照非專利文獻3)。另一方面，炎性反應（inflammatory)疾病，例如，接觸性皮膚炎（出療）、乾癬' 尋常性天疱瘡（pemphigus vulgaris)、其他伴隨粗糙皮膚的各種皮膚疾病等的原因及發病機制為各式各樣。就此原因而言，已知係來自透明質酸酶活性。 201225989 於活體組織保持親和性的透由紫外線、酵素等而被分解，伴.=絲含水系中經水效果。又’透明質酸係存在於:^董的降低亦減少保 #祕古Μ χ 、、胞間組織，亦與血管透化，與自肥大細胞之脱顆粒有關。因此，精=制=質酸之水解酵素的透明質酸酶活性，祈求透月貝酉义之安定化’防止自肥大細胞之細織胺等化學媒介物的釋放’可期待保濕之強化及抗炎症。作為具有如此透明質酸酶活性抑制作用的草本藥 (herbal medicine)而言，已報告例如，金錦香屬（〇sbeckia) 植物之萃取物（參照專利文獻4)、藤茶萃取物（參照專利文獻5)、迷迭香萃取物、百里香（thyme)萃取物及山薄荷屬（Melissa)萃取物（參照專利文獻6)等。又’表皮經由角化細胞之分裂及之後的分化，經常地產生新的角質細胞，而具有防止皮膚免於外界各種刺激的防禦機能。尤其，於角化細胞之分化過程，自有棘層至顆粒層，内皮蛋白（involucrin)等之蛋白質會表現，藉由酵素麩醯胺轉移酶-1之作用而被交聯’形成包埋角化細胞的不溶性細胞膜樣構造體的角質化包膜（cornified enVek)pe (CE))，而賦予角質細胞之細胞骨格及構造的安定性。然而，因各式各樣的因素，表皮中的麩醯胺轉移酶_1 之產生量減少，角質化包膜（CE)形成會成為不完全的狀態，角化變的不能立·常地進行。其結果，一 t5忍為會隻成S 3 201225989 (refer to Non-Patent Document 2). Further, it causes a decrease in the amount of hyaluronic acid produced as a natural moisturizing factor. As a result, aging symptoms such as moisturizing function or elasticity of the skin, abnormal peeling of keratin, wrinkles, dullness, loss of texture, and reduction in elasticity are exhibited. Therefore, it is generally believed that the promotion of skin aging by preventing the production of laminin-5 and the production of hyaluronic acid. Therefore, it is expected that a natural substance having high safety can obtain a substance having a laminin-5 production promoting action and hyaluronic acid production promoting action. Heretofore, in the case of having a laminin-5 production promoting effect, for example, Puerariae radix extract, licorice extract or flavonoid thereof, and fan (Iris domestica) have been reported. Extract, Blackstone tree (Alstoniascholaris) extract, Tenosporacordifolia extract, fenugreek extract, whey extract (above, refer to Patent Document 1), red stalk flower (Ononis) The spinosa) extract, the Medicagofalcata extract, the bean sprout% extract, the Vigna angularis extract (refer to Patent Document 2 above), the soybean extract (see Patent Document 3), and the like. In addition, it is attempted to extract a substance having a hyaluronic acid production promoting action from a natural product (see Non-Patent Document 3). On the other hand, inflammatory diseases, for example, contact dermatitis (therapy), cognac pemphigus vulgaris, other skin diseases associated with rough skin, and pathogenesis are Everything is different. For this reason, it is known to be derived from hyaluronidase activity. 201225989 The affinity of the living tissue is decomposed by ultraviolet rays, enzymes, etc., with the effect of water in the water-containing system. Further, hyaluronic acid is present in: ^The reduction of Dong also reduces the preservation of the secrets, the intercellular tissues, and the vascular permeability, and the degranulation of large cells from mast cells. Therefore, the hyaluronidase activity of the hydrolyzed enzyme of the refined acid system is prayed for the stability of the chemical mediators such as fine-striped amines from the large cells of the fat cell, and the anti-moisturizing and anti-moisture can be expected. Inflammation. As a herbal medicine having such a hyaluronic acid activity inhibitory action, for example, an extract of a genus sbeckia plant (see Patent Document 4) and a rattan tea extract have been reported (refer to Patent Document 5). ), rosemary extract, thyme extract, and Melissa extract (see Patent Document 6). Further, the epidermis frequently produces new keratinocytes through the division and subsequent differentiation of keratinocytes, and has a defense function of preventing skin from being stimulated by various external stimuli. In particular, in the differentiation process of keratinocytes, proteins from the spine layer to the granular layer, involucrin, etc., are expressed by the action of the enzyme branamine transferase-1 to form an embedding angle. The cornified enVek pe (CE) of the insoluble cell membrane-like construct of the cell, which confers stability to the cell bone and structure of the keratinocytes. However, due to various factors, the amount of production of the branamine transferase_1 in the epidermis is reduced, and the formation of the keratinized envelope (CE) becomes incomplete, and the keratinization cannot be performed constantly. . As a result, a t5 will endure only

S 5 201225989 壬現如角質障壁機能降低及皮膚保濕機能降低、粗链皮膚或乾燥肌膚等之皮膚症狀。月由於如此情形’-般認為藉由提高角化細胞之表皮中的麵酿胺轉移酶]之產生，促進角質化包膜之形成而將角 ^正常化’可抑料隨乾燥或紫外料之外部刺激之皮膚 P早壁機能的降低’可預防及改善肌膚乾燥或_皮膚等各式各樣的皮膚症狀。於皮膚之黑色素亦有達成保護身體免於紫外線傷害的任務’但過度生成或不均—的蓄制成為皮膚的黑化甚至斑點之原因。一般而言’係經由色素細胞中所生合成的酵素酪胺酸酶（tyr0Sinase)之作用，而由酪胺酸變成如叫（二經基苯丙胺酸）、由dopa變成多巴醌（d〇paquin〇ne)，其次，經由5,6-二羥基靛酚（ind〇phen〇1)等之中間體而形成黑色素。因此，為了預防及改善皮膚之顏色變黑（皮膚色素沈著症），即為了美白而抑制黑色素之產生、或將已經產生的黑色素淡色漂白一般認為係有效的。由於如此之背景，抑制黑色素產生而導致美白效果的物質之開發變的如此盛行。毛髮之成長係依由成長期、退行期、休止期而成的週期的毛髮週期（hair cycle)而重複進行成長及脱落。此毛髮週期中，由休止期到成長期之新毛包形成的階段，一般認為係成長毛髮中最重要者。而’負責此階段中的毛包上皮系細胞之增殖及分化上重要任務者，一般認為係毛乳頭細胞。毛乳頭細胞係位於毛根附近由外毛根鞘細胞及基質 201225989 細胞所構成的毛包上皮系細胞之内側中，位於包埋於基底膜的毛根之根幹部分的細胞，助長毛包上皮系細胞而促進其增殖等，於毛髮之分化上負責重要的任務（參照非專利文獻4 )。另一方面，已有報告含有鳳梨果實搾汁液或鳳梨果實萃取物作為美白、美肌成分的皮膚外用劑（參照專利文獻 7)。然而，於安全性及生產性優異之可日常攝取且為便宜，同時具有優異的層黏連蛋白-5產生促進作用、表皮透明質酸產生促進作用、透明質酸酶活性抑制作用、麩醯胺轉移酶-1產生促進作用、黑色素產生抑制作用、及毛乳頭細胞增殖促進作用之至少任一者的天然系之各種製劑的需要者之祈盼極微強烈，又不能提供可充分滿足之物係為現狀。 [先前技術文獻] 專利文獻 PTL1特開2003-137767號公報 PTL2特開2003-137768號公報 PTL3特開2004-217618號公報 PTL4特開2003-055242號公報 PTL5特開2003-012532號公報 PTL6特開平8-333267號公報 PTL7特開2002-275081號公報S 5 201225989 The skin symptoms such as keratin barrier function reduction and skin moisturizing function reduction, thick chain skin or dry skin. Because of this situation, it is thought that by increasing the production of the flavonoid transferase in the epidermis of the keratinocytes, the formation of the keratinized envelope is promoted and the angle is normalized, which can be suppressed with the dry or ultraviolet material. The externally stimulating skin P's early wall function can reduce and prevent various skin symptoms such as dry skin or _ skin. The melanin on the skin also has the task of protecting the body from UV damage, but excessive production or unevenness is the cause of blackening or even spots on the skin. In general, 'by the action of the enzyme tyrosinase (tyr0Sinase) synthesized in the pigment cells, from tyrosine to so-called (dimethoprim), from dopa to dopaquin (d〇paquin) 〇ne), followed by formation of melanin via an intermediate such as 5,6-dihydroxyindophenol (ind〇phen〇1). Therefore, in order to prevent and improve the color darkening of the skin (skin pigmentation), that is, to suppress the production of melanin for whitening, or to bleach the already produced melanin, it is generally considered to be effective. Because of this background, the development of substances that inhibit the production of melanin and cause whitening effects has become so popular. The hair growth is repeated by the hair cycle of the growth period, the regression period, and the rest period. In this hair growth cycle, the stage of formation of new hairs from the rest period to the growth stage is generally considered to be the most important one in growing hair. The nipple cells are generally considered to be responsible for the important tasks of proliferation and differentiation of hair follicle cells in this stage. The dermal papilla cell line is located in the inner side of the hairy epithelial cells composed of outer root sheath cells and matrix 201225989 cells near the hair root, and is located in the root part of the hair root embedded in the basement membrane, which promotes hair follicle epithelial cells and promotes The proliferation and the like are responsible for important tasks in the differentiation of hair (see Non-Patent Document 4). On the other hand, it has been reported to contain an extract of pineapple fruit juice or an extract of pineapple fruit as a skin external preparation for whitening and skin component (refer to Patent Document 7). However, it is excellent in safety and productivity, and can be taken daily and is inexpensive, and has excellent laminin-5 production promoting action, epidermal hyaluronic acid production promoting action, hyaluronidase activity inhibiting effect, and branamide The need for the various preparations of the natural system of at least one of the transferase-1 production-promoting action, the melanin production-inhibiting action, and the dermal papilla cell proliferation-promoting action is extremely strong, and the satisfactory system is not provided as the present state. . [PTL1, JP-A-2003-137768, PTL No. 2003-217618, PTL No. 2003-055242, PTL5, JP-A-2003-012532, PTL6, Special Opening Japanese Patent Publication No. 8-333267 (PTL7) No. 2002-275081

S 非專利文獻 7 201225989 NPLl Marinkovich MP et al. 5 J. Cell. Biol. 1992 199 · 695-703 NPL2 Lavker et al. » J. Invest. Dermatol. 1979 73 : 59-66 NPL3 「Fragrance Journal」1992 年、No.ll，p43 NPL4 「Trends Genet」，1992 年，第 8 卷，p.56-61 【發明内容】發明摘述技術問題本發明係以解決向來之前述諸問題，達成以下目的為課題。即，本發明係以提供安全性及生產性優異的可日常攝取、且便宜，同時具有優異的層黏連蛋白_5產生促進作用、表皮透明質酸產生促進作用、透明質酸酶活性抑制作用、麩醯胺轉移酶]I生促進作用、黑色素產生抑制作用、及毛乳頭細胞增殖促進作用之至少任—者的不含有糖之鳳梨萃取物、及其製造方法為目的。又’本發明係以提供具有優異的層黏連蛋白·5產生促進作用、安全性高的層轉蛋白_5產生促進劑為目的。又，本發明係以提供具有優異的表皮透明質酸產生促進作用、安全性高的表皮透明質酸產生促進劑為目的。χ，本發明係以提供具有優異的it日謂酸_活性抑制作用、安全性高的透明質酸酶活性抑制劑為目的。又，本發明係以提供具有優異祕㈣轉移^產生促進仙、安餘高的麟 8 2 201225989 胺轉移酶-1產生促進劑為目優異的黑色素產生抑制作用…X ’本發明係以提供具有劑為目的。又，本發明係：：：高的黑色素產生抑制殖作用、安全性高的錢SI=料優異的毛乳頭細胞增本發明係以提供-種特徵為含二殖促進劑為目的。又’ 物的飲食品、皮膚外用劑'及^迷不含有轉之鳳梨萃取解決問題之手段 $ &長劑為目的° 為了解決前述課題，本發明果 =以下的見解，’發現藉由溶齊™之可食部而獲仔的鳳梨卒取物具有優異的層勒連蛋白產生促進作用表皮透明貝酉义產生促進作用、透明質酸酶活性抑制作用、麵醯胺轉移酶·i產生促進仙、黑色素產生抑制作用、及毛礼頭細胞增殖促進作用，故前述鳳梨萃取物適合作為層黏連蛋白_5產生促㈣、表皮透明質酸產生促進劑、透明質酸酶活性抑制劑、楚醯胺轉移酶·丨產生促進劑、黑色素產生抑制、及毛乳頭細胞增殖促進劑、以及飲食品、皮膚外用劑、及毛髮生長劑之用途。〜本發明縣發明相基於前述見解，就作為用以解決刖述課題的手段而言，有如以下各點。即，〈:广-種不含有糖之鳳梨萃取物，其特徵為其係藉 /合劑卒取鳳梨之可食部而獲得，且不含有葡萄糖及果糖。係^!^如前述<1;>記載之不含有糖之风梨萃取物，其 …合μ卒取鳳梨可食部之麗雜的殘渣而獲得。 9 201225989 <3>如前述至<2> 之鳳梨萃取物，其項;己載之不含有糖溶液。 7G體積之乙醇水 <心一種不含有糖之鳳梨萃取物徵為取鳳梨可食部，除去葡萄糖及果糖。製造方法，=Γ：：㈣之不含妹之難萃取物之 <6>如别述<4>至<5>中任 - :=，方法’其㈣⑼=::: 如前=>:種促進劑’其特徵為含有物。、°載之不含有糖之鳳梨萃取 :二 =-=== <9> -種透明質酸酶活性抑制劑 :述❻至<3>中任1記載之不含有糖= <10>種楚酿胺轉移酶_i 有如前述蜊，其特徵為含取物。中任—項記載之不含有糖之鳳梨萃 <二:3色:產生抑制劑，其特徵為含有如前述 U〉中任—項記載之不含有糖之鳳梨萃取物。 201225989 <12> —種毛乳頭細胞增殖促進劑，其特徵為含有如前述<1>至<3>中任一項記載之不含有糖之鳳梨萃取物。 <13> —種飲食品，其特徵為含有如前述<1>至<3 >中任一項記載之不含有糖之鳳梨萃取物。 < 14> 一種皮膚外用劑，其特徵為含有如前述< 1>至 <3>中任一項記載之不含有糖之鳳梨萃取物。 <15> —種毛髮生長劑，其特徵為含有如前述<1>至 <3>中任一項記載之不含有糖之鳳梨萃取物。發明之優異效果依據本發明之不含有糖之鳳梨萃取物、及其製造方法，可提供安全性及生產性優異之可日常攝取、且便宜的同時，提供具有優異的層黏連蛋白-5產生促進作用、表皮透明質酸產生促進作用、透明質酸酶活性抑制作用、麩醯胺轉移酶-1產生促進作用、黑色素產生抑制作用、及毛乳頭細胞增殖促進作用之至少任一者之不含有糖之鳳梨萃取物、及其製造方法。依據本發明之層黏連蛋白-5產生促進劑，可提供解決向來之諸問題，且具有優異的層黏連蛋白-5產生促進作用、安全性高的層黏連蛋白-5產生促進劑。依據本發明之表皮透明質酸產生促進劑，可提供解決向來之諸問題，且具有優異的表皮透明質酸產生促進作用、安全性高的表皮透明質酸產生促進劑。依據本發明之透明質酸酶活性抑制劑，可提供解決向 201225989 來之諸問題，且具有優異的透明質酸酶活性抑制作用、安全性高的透明質酸酶活性抑制劑。依據本發明之麩醯胺轉移酶-1產生促進劑，可提供解決向來之諸問題，且具有優異的麵醯胺轉移酶-1產生促進作用、安全性高的麩醯胺轉移酶-1產生促進劑。依據本發明之黑色素產生抑制劑，可提供解決向來之諸問題，且具有優異的黑色素產生抑制作用、安全性高的黑色素產生抑制劑。依據本發明之毛乳頭細胞增殖促進劑，可提供解決向來之諸問題，且具有優異的黑毛乳頭細胞增殖作用、安全性高的毛乳頭細胞增殖促進劑。【實施方式】具體例之說明 (鳳梨）嚴梨（Pinapple)係屬於鳳梨科鳳梨屬之多年生植物，學名為：Ananas comosus ( L. ) Merr.或 Ananas sativus Schult，於中國亦稱為鳳梨。果實為大角形且多肉、黃色且成熟放出芳香味，可作為食用物來使用。鳳梨的產地主要為美國、菲律賓、馬來西亞、巴西、澳洲等，但於獲得用於本發明的萃取物上並未特別限定其種類或產地。 (不含有糖之鳳梨萃取物糖、及不含有鳳梨萃取物之製造方法）本發明之不含有糖之鳳梨萃取物可藉由溶劑自作為萃s 12 201225989 取原料之梨之可食部萃取而獲得。該*含有糖之鳳梨取物以不含葡萄糖及果糖為必要。〜本，明之不含有糖之鳳梨萃取物之製造方法係包含藉由’合d萃取鳳梨可食部’而去除葡萄糖及果糖，更因應必要包含適宜選擇的其他步驟。、就如述不含有糖之鳳梨萃取物之萃取原料而言，只要為果肉、果芯部（芯）等之鳳梨可食部即可，並未特別限制，可因應目的而適宜選擇，但鳳梨可食部壓搾後之殘渣，即採取鳳梨果汁後殘留的纖維質（鳳梨肉質部（pulp )) 為特佳。前述萃取原料係使用採取果汁後洗淨、乾燥、粉碎者為較佳。其中’乾燥可使用日光來進行，亦可使用通常使用的乾燥機來進行。剷述不含有糖之鳳梨萃取物可經由將前述鳳梨可食部投入前述溶劑中，於室溫至溶劑沸點以下的溫度，使用住意裝置來萃取而容易地獲得。就前述溶劑而言，並未特別限制，可因應目的而適宜選擇’例如，可舉例甲醇、乙醇、丙醇、異丙醇等之碳數 1〜5的低級脂肪族醇；丙酮、甲基乙基酮等之低級脂肪埃酮；1，3-丁二醇、丙二醇、甘油等之碳數2〜5的多價醇；己烧；低級脂肪族醇、低級脂肪族酮、多價醇等之親水性有機溶劑與水之混合溶劑等。此等中，以親水性有機溶劑與水之混合溶劑為較佳。就前述親水性有機溶劑而言，並未特別限制，可因應目的而適宜選擇，但曱醇、乙醇、丙醇、 13 201225989 1,3-丁二醇、甘油、丙二醇等為較佳，乙醇為更佳。於水與親水性有機溶劑之混合溶劑，使用低級醇作為前述親水性有機溶劑的情形，就前述低級脂肪族醇之於前述混合溶劑的含量而言，10體積％〜1〇〇體積％為較佳，70 體積％〜100體積％為更佳，90體積％為特佳。使用低級脂肪族酮作為前述親水性有機溶劑的情形，就前述低級脂肪族酮之於前述混合溶劑的含量而言，10體積％〜80體積％為較佳。使用多價醇作為前述親水性有機溶劑的情形，就前述多價醇之於前述混合溶劑中的含量而言，1〇體積％〜9〇體積％為較佳。就前述不含有糖之鳳梨萃取物之萃取方法而言，只要使前述鳳梨可食部中所含的脂溶性成分於前述溶劑溶出為可能即可，並未特別限定，可依據常法進行。又，萃取處理之際，採用特殊的萃取方法並非必要，可於室溫至回流加熱下使用任意之裝置。具體而言，就前述不含有糖之鳳梨萃取物之萃取方法而言，例如，可舉例於注滿乙醇水溶液等之前述溶劑的處理槽中，投入壓搾鳳梨可食部後之殘渣（鳳梨肉質部）等之前述萃取原料，因應必要一邊適當攪拌，一邊以回流萃取器於80°C加熱萃取2小時，加熱時過濾而將脂溶性成分溶出後，使用蒸發器（evaporator )，減壓下濃縮，再進行相同的過濾處理，獲得的中間萃取物以水洗淨，經由去除葡萄糖及果糖等之糖質，而獲得作為目的物之不含有糖之鳳梨萃取物的方法。 14 201225989 此時，萃取條件因應前述萃取原料等可適當調整，但前述萃取溶劑量，相對於作為前述萃取原料之鳳梨可食部，以5倍量〜20倍量（質量比）為較佳，萃取時間係1 小時〜3小時為較佳，萃取溫度係常溫〜95°C為較佳。又，獲得的前述不含有糖之鳳梨萃取物，為了獲得前述不含有糖之鳳梨萃取物之稀釋物、濃縮物、乾燥物、粗純化物、純化物等，依據通常方法亦可施予稀釋、濃縮、乾燥、純化等之處理。又，獲得的前述不含有糖之鳳梨萃取物，亦可直接使用作為層黏連蛋白-5產生促進劑、表皮透明質酸產生促進劑、透明質酸酶活性抑制劑、麩醯胺轉移酶-1產生促進劑、黑色素產生抑制劑、毛乳頭細胞增殖促進劑之任一者，於容易利用的點，以前述濃縮液、前述乾燥物為較佳。於獲得前述乾燥物時，為了改善吸濕性，亦可添加糊精 (dextrin)、環糊精（cyclodextrin)等之載劑（carrier)。 < <不含有之成分> > 於本發明，「不含有糖」之「糖」係意指葡萄糖及果糖。又，「不含有」、「不含有糖」、「去除糖」係意指各糖之相對於前述不含有糖之鳳梨萃取物的含量為0.5質量％以下，但 0.2質量％以下為較佳。前述葡萄糖及果糖之含量，例如，可使用葡萄糖/果糖分析套組（F-套組（葡萄糖/果糖），J.K. International股份有限公司製），依據套組所附的說明書來測量。又，就被驗試料而言，可使用將前述不含有糖之鳳梨萃取物溶解於50 s 15 201225989 體積％乙醉水溶液中成為樣品濃度為5體積％者。 (層黏連蛋白-5產生促進劑、表皮透明質酸產生促進劑、透明質酸酶活性抑制劑、麩醯胺轉移酶-1產生促進劑、黑色素產生抑制劑、毛乳頭細胞增殖促進劑）本發明之層黏連蛋白-5產生促進劑係含有前述不含有糖之鳳梨萃取物而成，更因應必要為含有其他成分而成。本發明之表皮透明質酸產生促進劑係含有前述不含有糖之鳳梨萃取物而成，更因應必要為含有其他成分而成。本發明之透明質酸酶活性抑制劑係含有前述不含有糖之鳳梨萃取物而成，更因應必要含有其他成分而成。本發明之麩醯胺轉移酶_1產生促進劑係含有前述不含有糖之鳳梨萃取物而成，更因應必要含有其他成分而成。本發明之黑色素產生抑制劑係含有前述不含有糖之鳳梨萃取物而成，更因應必要含有其他成分而成。本發明之毛乳頭細胞增殖促進劑係含有前述不含有糖之鳳梨萃取物而成，更因應必要含有其他成分而成。本發明之層黏連蛋白-5產生促進劑係可僅含有前述不含有糖之鳳梨萃取物而成者，亦可含有其他成分。就前述層黏連蛋白-5產生促進劑中之前述不含有糖之鳳梨萃取物之含量而言，並未特別限制，可因應目的而適宜選擇。本發明之表皮透明質酸產生促進劑係可僅含有前述不含有糖之鳳梨萃取物而成，亦可含有其他成分。就前述表皮透明質酸產生促進劑中之前述不含有糖之 16 201225989 鳳梨萃取物之含量而言，並未特別限制，可因應目的而適宜選擇。本發明之透明質酸酶活性抑制劑係可僅含有前述不含有糖之鳳梨萃取物而成，亦可含有其他成分。就前述透明質酸酶活性抑制劑中之前述不含有糖之鳳梨萃取物之含量而言，並未特別限制，可因應目的而適宜選擇。本發明之麩醯胺轉移酶-1產生促進劑係可僅含有前述不含有糖之鳳梨萃取物而成者，亦可含有其他成分。就前述麩醯胺轉移酶-1產生促進劑中之前述不含有糖之鳳梨萃取物之含量而言，並未特別限制，可因應目的而適宜選擇。本發明之黑色素產生抑制劑係可僅含有前述不含有糖之鳳梨萃取物而成者，亦可含有其他成分。就前述黑色素產生抑制劑中之前述不含有糖之鳳梨萃取物之含量而言，並未特別限制，可因應目的而適宜選擇。本發明之毛乳頭細胞增殖促進劑係可僅含有前述不含有糖之鳳梨萃取物而成者，亦可含有其他成分。就前述毛乳頭細胞增殖促進劑中之前述不含有糖之鳳梨萃取物之含量而言，並未特別限制，可因應目的而適宜選擇。 <其他成分> 前述不含有糖之鳳梨萃取物係可使用糊精、環糊精等之藥學上可容許的載劑之外的其他任意辅助劑，依據通常 17 201225989 方法，製劑化成粉末狀、顆 , , ^ 稍极狀、錠劑狀、液狀等之任咅劑型來提供，除了可摻合於 ..x 、/、他組成物（例如，經口醫藥品4 )來使用之外，可作’、 F巧軟貧劑、外用液劑、貼附劑箕來使用。此時，就輔助劑而a ’叨^向5，例如，可使用賦形劑、社合劑、崩壞劑、潤滑劑、皋中如 ^ ^ ^ 、口 J 女又劑、矯味劑、矯臭劑等。就前述賦形劑而言，例如，可舉例乳糖、白糖、氣化納、葡萄糖、殿粉、碳酉_、高嶺土、微結晶纖維素、石夕酸等。=，就前述結合劑而言，例如，可舉例水、乙醇、丙醇、單糖漿、葡萄糖液、殿粉液、明膠液、叛基甲基纖維素、羥基丙基纖維素、羥基丙基澱粉、甲基纖維素、乙基纖維素、蟲膠（shellac )、磷酸鈣、聚乙烯呢咯啶酮 (polyvinyl pyrrolidone)等。就前述崩壞劑而言，例如，可舉例乾燥澱粉、藻酸鈉、瓊脂末、碳酸氫鈉、碳酸妈、月桂基硫酸鈉、硬脂酸單甘油酯、乳糖等。就前述潤滑劑而言，例如，可舉例純化滑石、硬脂駿鹽、硼砂、聚乙二醇等。就前述安定化劑而言，例如，可舉例焦亞硫酸鈉 (sodium pyrosulfite )、EDTA、疏乙酸（thioglycolic acid )、琉乳酸等。就前述橋味劑及矯臭劑而言，例如，可舉例白糖、橙皮、檸檬酸、酒石酸等。又，本發明之層黏連蛋白-5產生促進劑、表皮透明質酸產生促進劑、透明質酸酶活性抑制劑、麩醯胺轉移酶-1 產生促進劑、黑色素產生抑制劑、及毛乳頭細胞增殖促進劑，因應必要，可與具有層黏連蛋白-5產生促進作用、表皮透明質酸產生促進作用、透明質酸酶活性抑制作用、麩 201225989 醯胺轉移酶-1產生促進作用、黑色素產生抑制作用、及毛乳頭細胞增殖促進作用任一者之其他天然萃取物等一起摻合使用。前述層黏連蛋白-5產生促進劑藉由含有作為有效成分之前述不含有糖之鳳梨萃取物之作用，而發揮層黏連蛋白 -5產生促進作用。前述表皮透明質酸產生促進劑藉由含有作為有效成分之前述不含有糖之鳳梨萃取物之作用，而發揮表皮透明質酸產生促進作用。前述透明質酸酶活性抑制劑藉由含有作為有效成分之前述不含有糖之鳳梨萃取物之作用，而發揮透明質酸酶活性抑制作用。前述麩醯胺轉移酶-1產生促進劑藉由含有作為有效成分之前述不含有糖之鳳梨萃取物之作用，而發揮麩醯胺轉移酶-1產生促進作用。前述黑色素產生抑制劑藉由含有作為有效成分之前述不含有糖之鳳梨萃取物之作用，而發揮黑色素產生抑制作用。前述毛乳頭細胞增殖促進劑藉由含有作為有效成分之前述不含有糖之鳳梨萃取物之作用，而發揮毛乳頭細胞增殖促進作用。以本發明之層黏連蛋白-5產生促進劑，經由優異的層黏連蛋白-5產生促進作用，例如，促進皮膚之基底膜形成，使預防及改善皺紋、彈力性的降低等皮膚老化症狀成為可s 19 201225989 能。惟，本發明之層黏連蛋白-5產生促進劑除了此等用途以外，可使用於具有發揮層黏連蛋白-5產生促進作用的意義的全部用途。以本發明之表皮透明質酸產生促進劑，經由優異的表皮透明質酸產生促進作用，例如，使保濕力、彈力性提升，使預防及改善粗糙皮膚、皺紋、彈力性的降低等皮膚老化症狀成為可能。惟，本發明之表皮透明質酸產生促進劑除了此等用途以外，可使用於具有發揮表皮透明質酸產生促進作用的意義的全部用途。以本發明之透明質酸酶活性抑制劑，經由優異的透明質酸酶活性抑制作用，例如，使抑制透明質酸之分解、促進組織胺游離、圖謀保濕之強化、預防及改善炎症或過敏成為可能。惟，本發明之透明質酸酶活性抑制劑除了此等用途以外，可使用於具有發揮透明質酸酶活性抑制作用的意義的全部用途。以本發明之麩醯胺轉移酶-1產生促進劑，經由優異的麵醯胺轉移酶-1產生促進作用，例如，使皮膚表皮之障壁機能提升、粗糙皮膚紋理的消失、預防及改善彈力性的降低等之老化症狀成為可能。惟，本發明之麩醯胺轉移酶-1 產生促進劑除了此等用途以外，可使用於具有發揮麩醯胺轉移酶-1產生促進作用的意義的全部用途。以本發明之黑色素產生抑制劑，經由優異的黑色素產生抑制作用，例如，保護皮膚免於紫外線傷害、預防及改善皮膚之黑化、斑點等之老化症狀成為可能。惟，本發明e 20 201225989 之黑色素產生抑制劑除了此等用途以外，可使用於具有發揮黑色素產生抑制作用的意義的全部用途。以本發明之毛乳頭細胞增殖促進劑，經由優異的毛乳頭細胞增殖促進作用，例如，促進毛包上皮系細胞之增殖及毛髮之分化、預防及改善細毛化成為可能。惟，本發明之毛乳頭細胞增殖促進劑除了此等用途以外，可使用於具有發揮毛乳頭細胞增殖促進作用的意義的全部用途。本發明之層黏連蛋白-5產生促進劑、表皮透明質酸產生促進劑、透明質酸酶活性抑制劑、麩醯胺轉移酶-1產生促進劑、黑色素產生抑制劑、及毛乳頭細胞增殖促進劑具有優異的作用，同時係由可食用的鳳梨所獲得的萃取物，安全性為優異，故適合摻合於飲食品、皮膚外用劑、及毛髮生長劑。又，本發明之層黏連蛋白-5產生促進劑、表皮透明質酸產生促進劑、透明質酸酶活性抑制劑、麩醯胺轉移酶-1 產生促進劑、黑色素產生抑制劑、及毛乳頭細胞增殖促進劑因具有優異的作用，故可適當地利用作為層黏連蛋白 -5、表皮透明質酸、透明質酸酶、麩醯胺轉移酶-1、及毛乳頭細胞之研究用的試藥；透明質酸酶、層黏連蛋白-5、表皮透明質酸、麩醯胺轉移酶-1、黑色素、及毛乳頭細胞有關的機能及疾病之研究用的試藥。又，本發明之層黏連蛋白-5產生促進劑、表皮透明質酸產生促進劑、透明質酸酶活性抑制劑、麩醯胺轉移酶-1 產生促進劑、黑色素產生抑制劑、及毛乳頭細胞增殖促進 21 201225989 劑可適當地適用於人類，但只要能發揮各別作用效果，亦可使用於人類以外之動物。 (飲食品）本發明之飲食品係含有本發明之不含有糖之鳳梨萃取物、以及層黏連蛋白-5產生促進劑、表皮透明質酸產生促進劑、透明質酸酶活性抑制劑、麩醯胺轉移酶-1產生促進劑、黑色素產生抑制劑、及毛乳頭細胞增殖促進劑之至少任一者而成，更因應必要含有其他成分而成。前述飲食品係可將前述不含有糖之鳳梨萃取物摻合於不妨礙其活性的任意飲食品，亦可為將前述不含有糖之鳳梨萃取物作為主成分的飲食品。又，前述飲食品可為前述不含有糖之鳳梨萃取物者。前述飲食品對人類健康的危害的疑慮少，於通常之社會生活中，所謂經口或經消化道投與所攝取者，並未受限於行政區分上之食品、醫藥品、醫藥外用品等之區分，例如，意指包含經口攝取的一般食品、健康食品、保健機能食品、美容用食品、醫藥外用品、醫藥品等廣範圍者。又，本發明之飲食品經由形態加工成飲料、硬膠囊、軟膠囊、顆粒等之形狀，而可簡便地飲食，有可能利用於廣範圍。就前述飲食品而言，並未特別限制，可因應目的適當地選定，例如，清涼飲料、碳酸飲料、營養飲料、果實飲料、乳酸飲料等之飲料（包含此等飲料之濃縮液及調整用粉末）；冰漠淋、冰果汁粉（sherbet )、包1丨冰等之冷甜點； 22 201225989 騫麥麵、烏龍麵、空心麵、俊子皮、燒賣的皮、中華麵、泡麵等之麵類；糖、糖果、口香糖、巧克力、錠果、曰式點心、餅乾、果膠、果凍、乳霜、烤餅乾、麵包等之點心類；烤蟹、链魚、蛤、縮魚、沙丁魚、蝦、飛魚、鯖魚、蘇魚、牡螺、秋刀魚、烏賊、赤貝、扇貝、鮑魚、海膽、魚子醬、日本鮑等之水產物；魚板、火腿、香腸等之水產· 畜產加工食品；加工乳、發酵乳等之乳製品；沙拉油、天婦羅油、人造奶油（margarine )、蛋黃醬（mayonnaise)、油酥（shortening)、發泡用生奶（whipping cream)、調味醬（dressing)等之油脂及油脂加工食品；調味汁、燒烤醬等之調味料；咖喱、燉物、親子丼、粥、什錦粥、中華丼、豬肉丼、天丼、鰻魚丼、牛肉繪飯、御田燒、麻婆豆腐、牛丼、肉磐、蛋化湯、蛋包飯、俊子、燒賣、漢堡、肉丸等之料理包（retort pouch)食品；沙拉、潰物等之副食（惣菜）；各種形fe之健康•美容•營養補充食品；銳劑、顆粒劑、膠囊劑、飲料劑、含錠劑（troche )等之醫藥品、醫藥外用品等。又，前述飲食品並未限定於上述例示者。就前述飲食品中的前述不含有糖之鳳梨萃取物、層黏連蛋白-5產生促進劑、表皮透明質酸產生促進劑、透明質酸酶活性抑制劑、麩醯胺轉移酶_1產生促進劑、黑色素產生抑制劑、及毛乳頭細胞增殖促進劑之任一者的含量而言，係因應添加的飲食品而不同，無法―概而全地規定，但疑劑、膠囊劑等之情开》’換算為前述不含有糖之鳳斟萃取物之量以1質量％〜90質量％為較佳，其他飲食品中 ' 換 23 201225989 异中為釗述不含有糖之鳳梨萃取物之量以0〇〇1質量〇/〇〜％質量％為較佳。又，考慮添加對象飲食品的—般的攝取量，成人每一日之前述不含有糖之鳳梨萃取物之攝取量調製為約lmg〜l，000mg左右者為較佳。就前述其他成分而言，可舉例於製造前述飲食品時通常使用的輔助原料、添加物等。就前述辅助原料及添加物而言，並未特別限制，可因應目的適宜選擇’例如，可舉例葡萄糖、果糖、蔬糖、麥芽糖、山梨糖醇、蛇菊芽（stevi〇side)、甜茶苷（⑹、玉米糖聚、乳糖、檸檬酸、酒石酸、蘋果酸、琥站酸、乳酸、L-抗壞血酸、dl-a_生育酚、異抗壞血酸=(― erythc^ate)、甘油、內二醇、甘油脂肪酸醋、聚甘油脂肪酸酯.、蔗糖脂肪酸酯、山梨糖醇酐脂肪酸酯（s〇rbit⑽ acid ester)、阿拉伯膠（Arabic gum)、角叉菜聚糖 (謝ag_n)、絡蛋白（casein)、明膠、果膠（㈣㈤、瓊脂、維生素B類、煙鹼酸醯胺、泛酸鈣、胺基酸類、鈣鹽類、色素、香料、保存劑等。士發明之飲食品可日f性地經口攝取，藉由為有效成 == 梨萃取物之作用’可極為有效果地達成透明質 -秦舌性_作用、層輯蛋自·5產生促進作用、義轉移酶-1產生促進作用、及毛礼頭細胞增殖促進作用之至少任一者。处產峰久本《月之飲食品對於人類為適合使用者，但只要此產生各別作用效果，亦可適用於人触外之動物。 24 201225989 (皮膚外用劑）本發明之皮膚外用劑係含有本發明之不含有糖之鳳梨萃取物 '以及層黏連蛋白_5產生促進劑、表皮透明質酸產生促進劑、透明質酸酶活性抑制劑、麩醯胺轉移酶_丨產生促進劑、黑色素產生抑制劑、及毛乳頭細胞增殖促進劑之任一者而成，更因應必要含有其他成分而成。其中，前述皮膚外用劑係意指使用於皮膚的各種藥劑，就其區分而言並未特別制限，例如，包含廣範圍之皮膚化极料、醫藥外用品、醫藥品等。刖述皮膚外用劑係於不妨礙其活性下，可將前述不含有糖之鳳梨萃取物摻合於任意之皮膚外㈣，亦可為將前述不含有糖之鳳梨萃取物作為主成分的皮膚外用劑。又，别述皮膚外用劑亦可為前述不含有糖之鳳梨萃取物。就刖述皮膚外用劑而言，並未特別限制，可因應目的而適宜選擇’例如’可舉例軟f、_、乳液、美容液、洗液、面膜、粉底、唇膏、人浴劑、生髮劑（hairt〇nic)、護髮液（hair lotion)、肥皂、沐浴乳等。就前述其他成分而言’並未特別限制，可因應目的而適宜選擇製造皮膚相劑時通常使用的成分，例如，可舉例收_ '殺8、抗菌劑、美白劑、紫外線吸收劑、保濕劑、細胞賦活劑、抗老化劑、消炎·抗過敏劑、抗氧化劑、 /舌!·生氧去除J ’仏類、壤類、碳化氫類、脂肪酸類、醇類、酯類、界面活性劑、香料等。就前述皮膚外用劍Φ)乂 t ^中之别述不含有糖之鳳梨萃取物、 201225989 層黏連蛋白-5產生促進劑、表皮透明質酸產生促進劑、透明質酸酶活性抑制劑、麩醯胺轉移酶-1產生促進劑、黑色素產生抑制劑、及毛乳頭細胞增殖促進劑之任一者之含量而言，並未特別限制，可因應皮膚外用劑之種類等加以適當選擇，例如，換算為前述不含有糖之鳳梨萃取物之量，以0.0001質量％〜10質量％為較佳，0.001質量％〜5質量％為更佳。 (毛髮生長劑）本發明之毛髮生長劑係含有本發明之不含有糖之鳳梨萃取物、以及層黏連蛋白-5產生促進劑、表皮透明質酸產生促進劑、透明質酸酶活性抑制劑、麩醯胺轉移酶-1產生促進劑、黑色素產生抑制劑、及毛乳頭細胞增殖促進劑之任一者而成，更因應必要含有其他成分而成。前述毛髮生長劑係於不妨礙其活性下，可將前述不含有糖之鳳梨萃取物摻合於任意之毛髮生長劑，亦可為將前述不含有糖之鳳梨萃取物作為主成分的毛髮生長劑。又，前述毛髮生長劑亦可為前述不含有糖之鳳梨萃取物。以本發明之毛髮生長劑，經由優異的層黏連蛋白-5產生促進作用、表皮透明質酸產生促進作用、透明質酸酶活性抑制作用、麵醯胺轉移酶-1產生促進作用、黑色素產生抑制作用、及毛乳頭細胞增殖促進作用等，例如，促進乳頭細胞及毛包上皮系細胞之增殖、毛髮之分化等，可適當利用於用以預防及改善細毛化、脱毛等。又，本說明書中的「毛髮生長劑」係意指具有使毛髮 26 201225989 使:毛發:J持毛髮之健康狀態、預防税毛及本發明之毛㈣^ 藥劑，亦包含「養毛劑」。因此， t生長Μ亦可較佳作為養毛劑使用。皮膚之毛髮生長劑因具有優異的作用，同時適用於生“月二使用感及安全性優異’故摻合於頭髮用毛髮 ^、較化粧料等之皮膚外用劑為適合的。就前述皮 =、用劑之投與部位而言，例如’頭髮、頭髮之毛根、頭頭皮之皮脂腺等之頭部為較佳。就前述皮膚外用型而言，例如’可舉例軟膏、霜劑、洗液、凝膠、轉 sol)噴霧劑、溶液等。就頭髮化粧料而言，例如，可兴例生髮劑（hairtGnk)、保髮液（hairliquid)、洗髮精、^ 油（hair oil)、潤絲精等。就刖述毛髮生長劑中的前述不含有糖之鳳梨萃取物、層黏連蛋白-5產生促進劑、表皮透明質酸產生促進劑、透明質酸酶活性抑制劑、麵醯胺轉移酶“產生促進劑、黑色素產生抑制劑、及毛乳頭細胞增殖促進劑之任一者的含量而言，並未特別限制，可因應毛髮生長劑之種類等加以適當選擇，但例如，換算前述不含有糖之鳳梨萃取物之量，以0.0001質量％〜10質量％為較佳，〇〇〇1質量％〜5質量％為更佳。、° 實施例以下’呈示製造例及試驗例以更具體地說明本發明，但本發明並未受限於此等。 (製造例1 :不含有糖之鳳梨萃取物之製造）S Non-Patent Document 7 201225989 NPLl Marinkovich MP et al. 5 J. Cell. Biol. 1992 199 · 695-703 NPL2 Lavker et al. » J. Invest. Dermatol. 1979 73 : 59-66 NPL3 "Fragrance Journal" 1992 [No. 11, p43 NPL4 "Trends Genet", 1992, Vol. 8, p. 56-61 SUMMARY OF THE INVENTION Technical Problem The present invention has been made to solve the above-mentioned problems and achieve the following objects. That is, the present invention provides daily safety and productivity, is excellent in daily intake, and is inexpensive, and has excellent laminin_5 production promoting action, epidermal hyaluronic acid production promoting action, and hyaluronidase activity inhibiting action. The glutamine-transferase-I-promoting effect, the melanin production-inhibiting action, and the dermal papilla cell proliferation-promoting action are at least a sugar-free pineapple extract, and a method for producing the same. Further, the present invention has an object of providing a layer-transformer_5 production promoter having excellent laminin-5 production promoting action and high safety. Further, the present invention has an object of providing a skin hyaluronic acid production promoter having an excellent epidermal hyaluronic acid production promoting action and high safety. In other words, the present invention has an object of providing an inhibitor of hyaluronidase activity having an excellent inhibitory action of acid-activity and high safety. Further, the present invention provides an excellent melanin production inhibitory effect by providing a superior secret (4) transfer generation-promoting scent, and a high-enriched Lin 8 2 201225989 amine transferase-1 production promoter. For the purpose. Further, the present invention is:: high melanin production inhibiting action, high safety money SI = excellent dermal papilla cell growth The present invention is intended to provide a secondary growth promoter. In addition, 'food and beverages, skin external preparations' and ^ fans do not contain the method of extracting pineapple extract to solve the problem. The purpose of the long-acting agent is to reduce the above-mentioned problems. The present invention is the following findings, 'discovery by dissolution The pineapple extract obtained from the edible part of QiTM has excellent laminin production promoting effect, epidermal transglutamate production promotion, hyaluronidase activity inhibition, and indoleamine transferase·i production promotion Inhibition of melanin, melanin production, and cell proliferation promotion of Maotoutou, so the above pineapple extract is suitable as laminin_5 to promote (4), epidermal hyaluronic acid production promoter, hyaluronidase activity inhibitor, Chu The use of a guanamine transferase, a sputum production promoter, a melanin production inhibitor, a dermal papilla cell proliferation promoter, and a food or drink, a skin external preparation, and a hair growth agent. The present invention is based on the above findings, and is a means for solving the problems described above. That is, <: broad-type pineapple extract containing no sugar, which is characterized by being obtained by taking the edible part of the pineapple by the combination agent, and does not contain glucose and fructose. The system is as described in the above <1;>; the extract of the pear without sugar is obtained, and the mixture is obtained by taking the residue of the edible portion of the pineapple. 9 201225989 <3> The pineapple extract as described above to <2>, which does not contain a sugar solution. 7G volume of ethanol water < heart a pineapple extract containing no sugar. Take the pineapple edible part, remove glucose and fructose. Manufacturing method, =Γ:: (4) <6> without the sister's difficult extract, as described in <4> to <5> in -:=, method '(4)(9)=::: as before = >: Kind of accelerator' is characterized by a content. , 凤凤凤凤萃取萃取萃取萃取萃取萃取萃取萃取萃取萃取萃取萃取萃取萃取萃取萃取萃取萃取萃取萃取萃取萃取萃取萃取萃取萃取萃取萃取萃取萃取萃取萃取萃取萃取萃取萃取萃取萃取萃取萃取萃取萃取透明萃取萃取透明透明透明透明透明透明透明透明透明The cultivar-transferase _i is as described above and is characterized by an extract. An edible pineapple extract containing no sugar as described in the above-mentioned item - 2: 3 color: an inhibitor of production, characterized by containing a pineapple extract containing no sugar as described in the above-mentioned U>. 201225989 <12> A hairy nipple cell growth promoter comprising the pineapple extract containing no sugar as described in any one of the above <1> to <3>. <13> A food and drink product characterized by containing the pineapple extract containing no sugar as described in any one of the above <1> to <3>. <14> A skin external preparation containing the pineapple extract containing no sugar as described in any one of the above <1> to <3>. <15> A hair growth agent comprising the pineapple extract containing no sugar as described in any one of the above <1> to <3>. Advantageous Effects of Invention According to the sugar-free pineapple extract of the present invention and a method for producing the same, it is possible to provide daily neat intake, which is excellent in safety and productivity, and which is excellent in laminin-5 production. At least one of promotion, epidermal hyaluronic acid production promoting action, hyaluronidase activity inhibiting action, branamine transferase-1 production promoting action, melanin production inhibiting action, and dermal papilla cell proliferation promoting action are not contained Sugar pineapple extract and a method for producing the same. The laminin-5 production-promoting agent according to the present invention provides a laminin-5 production-promoting agent which solves the problems of the past and has excellent laminin-5 production-promoting action and high safety. According to the epidermal hyaluronic acid production promoter of the present invention, it is possible to provide an epidermal hyaluronic acid production promoter which has a problem of solving the problems of the epidermis and has an excellent hyaluronic acid production promoting effect and high safety. According to the hyaluronidase activity inhibitor of the present invention, it is possible to provide a hyaluronidase activity inhibitor which solves the problems of 201225989 and has excellent hyaluronidase activity inhibition and high safety. The branamine transferase-1 production promoter according to the present invention can provide the problem of solving the problems of the past, and has excellent meridamyrase-1 production promoting action and high safety of branamine transferase-1 production. Promoter. According to the melanin production inhibitor of the present invention, it is possible to provide a melanin production inhibitor which solves the problems of the past and has excellent melanin production inhibitory action and high safety. According to the dermal papilla cell proliferation accelerating agent of the present invention, it is possible to provide a dermal papilla cell proliferation accelerating agent which solves the problems of the past and has excellent black hair papilla cell proliferation and safety. [Embodiment] Description of Specific Example (Hawthorn) Pinaple is a perennial plant belonging to the genus Bromeliad, and its name is Ananas comosus (L.) Merr. or Ananas sativus Schult, also known as pineapple in China. The fruit is large-horned and fleshy, yellow and ripe to give off aroma and can be used as a food. The origin of the pineapple is mainly in the United States, the Philippines, Malaysia, Brazil, Australia, etc., but the type or place of production is not particularly limited in obtaining the extract for use in the present invention. (Manufacturing method of sugar-free pineapple extract sugar and no pineapple extract) The pineapple extract containing no sugar of the present invention can be extracted from the edible portion of the pear as a raw material by extracting s 12 201225989 by a solvent. obtain. The *-contained pineapple extract is essential for the absence of glucose and fructose. The present invention is directed to a method for producing a pineapple extract containing no sugar, which comprises removing the glucose and fructose by extracting the edible portion of the pineapple, and further includes other steps suitable for selection. In the case of the extractable raw material of the pineapple extract which does not contain the sugar, it is not particularly limited as long as it is a pineapple edible portion such as a pulp or a core portion (core), and may be appropriately selected depending on the purpose, but the pineapple is appropriately selected. The residue after pressing in the edible portion is particularly good in the fiber (pulp) remaining after the pineapple juice is taken. It is preferred that the above-mentioned extraction raw material is washed, dried, and pulverized by using a juice. Here, 'drying can be carried out using daylight, or it can be carried out using a dryer which is usually used. The pineapple extract containing no sugar can be easily obtained by introducing the pineapple edible portion into the solvent and extracting it at a temperature of from room temperature to the boiling point of the solvent or less using a living device. The solvent is not particularly limited, and may be appropriately selected depending on the purpose. For example, a lower aliphatic alcohol having a carbon number of 1 to 5 such as methanol, ethanol, propanol or isopropanol; acetone or methyl ethyl; a lower aliphatic ketone such as a ketone or the like; a polyvalent alcohol having a carbon number of 2 to 5 such as 1,3-butanediol, propylene glycol or glycerin; a calcined; a lower aliphatic alcohol, a lower aliphatic ketone, a polyvalent alcohol or the like. a mixed solvent of a hydrophilic organic solvent and water, and the like. Among these, a mixed solvent of a hydrophilic organic solvent and water is preferred. The hydrophilic organic solvent is not particularly limited and may be appropriately selected depending on the purpose, but decyl alcohol, ethanol, propanol, 13 201225989 1,3-butylene glycol, glycerin, propylene glycol, etc. are preferred, and ethanol is Better. In the case where a lower alcohol is used as the hydrophilic organic solvent in a mixed solvent of water and a hydrophilic organic solvent, 10% by volume to 1% by volume of the lower aliphatic alcohol in terms of the content of the mixed solvent is Preferably, 70% by volume to 100% by volume is more preferred, and 90% by volume is particularly preferred. In the case where a lower aliphatic ketone is used as the hydrophilic organic solvent, the content of the above-mentioned lower aliphatic ketone in the above mixed solvent is preferably from 10% by volume to 80% by volume. In the case where a polyvalent alcohol is used as the hydrophilic organic solvent, it is preferred that the content of the polyvalent alcohol in the mixed solvent is from 1% by volume to 9% by volume. The extraction method of the pineapple extract containing no sugar is not particularly limited as long as the fat-soluble component contained in the edible portion of the pineapple is eluted in the solvent, and can be carried out according to a usual method. Further, in the extraction process, it is not necessary to use a special extraction method, and any device can be used from room temperature to reflux heating. Specifically, the extraction method of the pineapple extract containing no sugar, for example, can be exemplified by the residue of the edible portion of the pineapple (the pineapple meat portion) in a treatment tank filled with the solvent such as an aqueous ethanol solution. The above-mentioned extraction raw material is heated and extracted at 80 ° C for 2 hours with a reflux extractor as necessary, and filtered to elute the fat-soluble component after heating, and then concentrated under reduced pressure using an evaporator. The same filtration treatment is carried out, and the obtained intermediate extract is washed with water, and a saccharide such as glucose or fructose is removed to obtain a target of a pineapple-free extract containing no sugar. In the case of the above-mentioned extraction raw material, the amount of the extraction solvent is preferably 5 times to 20 times (mass ratio) with respect to the pineapple edible portion as the extraction raw material. The extraction time is preferably from 1 hour to 3 hours, and the extraction temperature is preferably from room temperature to 95 ° C. Further, the obtained sugar-free pineapple extract obtained by the method may be diluted according to a usual method in order to obtain a diluted product, a concentrate, a dried product, a crude purified product, a purified product, and the like of the sugar-free pineapple extract. Concentration, drying, purification, etc. Further, the obtained sugar-free pineapple extract obtained can also be directly used as a laminin-5 production promoter, epidermal hyaluronic acid production promoter, hyaluronidase activity inhibitor, and branamide transferase- (1) One of a promoter, a melanin production inhibitor, and a dermal papilla cell proliferation promoter, and the concentrate and the dried product are preferably used at the point of easy use. When the dried product is obtained, a carrier such as dextrin or cyclodextrin may be added to improve the hygroscopicity. <<Components not contained>> In the present invention, "sugar" which does not contain sugar means glucose and fructose. In addition, the content of the sugar-free pineapple extract is 0.5% by mass or less, and preferably 0.2% by mass or less, in the case of "not containing", "excluding sugar", and "removing sugar". The content of the aforementioned glucose and fructose can be measured, for example, using a glucose/fructose analysis kit (F-kit (glucose/fructose), manufactured by J.K. International Co., Ltd.) according to the instructions attached to the kit. Further, in the case of the test sample, the above-mentioned sugar-free pineapple extract can be dissolved in a 50 s 15 201225989 vol% aqueous solution of immersion to a sample concentration of 5% by volume. (Laminin-5 production promoter, epidermal hyaluronic acid production promoter, hyaluronidase activity inhibitor, branamine transferase-1 production promoter, melanin production inhibitor, dermal papilla cell proliferation promoter) The laminin-5 production promoter of the present invention comprises the above-mentioned pineapple-free extract containing no sugar, and further contains other components as necessary. The epidermal hyaluronic acid production promoter of the present invention comprises the above-mentioned pineapple extract containing no sugar, and further contains other components as necessary. The hyaluronidase activity inhibitor of the present invention comprises the above-mentioned pineapple extract containing no sugar, and further contains other components as necessary. The branamine transferase_1 production promoter of the present invention comprises the above-mentioned sugar-free pineapple extract, and further contains other components as necessary. The melanin production inhibitor of the present invention comprises the above-mentioned extract of phoenix-free pear, and further contains other components as necessary. The dermal papilla cell proliferation promoting agent of the present invention comprises the above-mentioned pineapple extract containing no sugar, and further contains other components as necessary. The laminin-5 production promoting agent of the present invention may contain only the aforementioned pineapple extract containing no sugar, and may contain other components. The content of the aforementioned sugar-free pineapple extract in the above-mentioned laminin-5 production promoter is not particularly limited and may be appropriately selected depending on the purpose. The epidermal hyaluronic acid production promoter of the present invention may contain only the above-mentioned pineapple extract containing no sugar, and may contain other components. The content of the above-mentioned non-sugar-containing 16 201225989 pineapple extract in the above-mentioned epidermal hyaluronic acid production promoter is not particularly limited and may be appropriately selected depending on the purpose. The hyaluronidase activity inhibitor of the present invention may contain only the above-mentioned sugar-free pineapple extract, and may contain other components. The content of the aforementioned sugar-free pear extract in the above-mentioned hyaluronidase activity inhibitor is not particularly limited and may be appropriately selected depending on the purpose. The branamide transferase-1 production promoting agent of the present invention may contain only the above-mentioned pineapple-free extract containing no sugar, and may contain other components. The content of the aforementioned sugar-free pineapple extract in the branamine transferase-1 production promoter is not particularly limited and may be appropriately selected depending on the purpose. The melanin production inhibitor of the present invention may contain only the above-mentioned pineapple extract containing no sugar, and may contain other components. The content of the aforementioned sugar-free extract of the pineapple in the melanin production inhibitor is not particularly limited and may be appropriately selected depending on the purpose. The dermal papilla cell proliferation promoting agent of the present invention may contain only the above-mentioned sugar-free pineapple extract, and may contain other components. The content of the aforementioned sugar-free pear extract in the dermal papilla cell proliferation promoter is not particularly limited and may be appropriately selected depending on the purpose. <Other components> The pineapple extract containing no sugar can be prepared into a powder according to the usual method of 201220128, using any adjuvant other than the pharmaceutically acceptable carrier such as dextrin or cyclodextrin. , granules, , ^ are provided in a slightly polar form, a lozenge form, a liquid form, etc., except that they can be blended with ..x, /, his composition (for example, oral medicine 4) , can be used as ', F smart soft agent, external liquid agent, patch 箕 to use. In this case, the auxiliary agent is a '叨5, for example, an excipient, a community agent, a disintegrating agent, a lubricant, a sputum, a scent, a flavoring agent, a flavoring agent, and the like can be used. Wait. With respect to the aforementioned excipients, for example, lactose, white sugar, sodium hydride, glucose, house powder, carbon 酉, kaolin, microcrystalline cellulose, oxalic acid and the like can be exemplified. =, in the case of the aforementioned binding agent, for example, water, ethanol, propanol, monosaccharide syrup, glucose solution, dinosaur solution, gelatin solution, thiomethylcellulose, hydroxypropylcellulose, hydroxypropyl starch , methyl cellulose, ethyl cellulose, shellac, calcium phosphate, polyvinyl pyrrolidone, and the like. As the above-mentioned breaker, for example, dried starch, sodium alginate, agarose, sodium hydrogencarbonate, carbonic acid mother, sodium lauryl sulfate, stearic acid monoglyceride, lactose or the like can be exemplified. As the aforementioned lubricant, for example, talc, hard fat salt, borax, polyethylene glycol or the like can be exemplified. As the stabilizer, for example, sodium pyrosulfite, EDTA, thioglycolic acid, hydrazine lactic acid, or the like can be exemplified. As the aforementioned odorant and the odorant, for example, white sugar, orange peel, citric acid, tartaric acid or the like can be exemplified. Further, the laminin-5 production promoter, the epidermal hyaluronic acid production promoter, the hyaluronidase activity inhibitor, the branamine transferase-1 production promoter, the melanin production inhibitor, and the dermal papillae of the present invention Cell proliferation promoter, if necessary, can promote laminin-5 production, epidermal hyaluronic acid production promotion, hyaluronidase activity inhibition, bran 201225989 indoleamine transferase-1 production promotion, melanin Other natural extracts or the like which produce an inhibitory action and promote the proliferation of dermal papilla cells are used together. The laminin-5 production-promoting agent exerts a laminin-5-promoting action by containing the aforementioned sugar-free pineapple extract as an active ingredient. The epidermal hyaluronic acid production promoter exerts an epidermal hyaluronic acid production promoting action by containing the above-mentioned sugar-free pineapple extract as an active ingredient. The hyaluronidase activity inhibitor exerts a hyaluronidase activity inhibitory action by containing the above-mentioned sugar-free pineapple extract as an active ingredient. The branamine transferase-1 production promoter exerts a branamine transferase-1 production-promoting action by containing the aforementioned sugar-free pineapple extract as an active ingredient. The melanin production inhibitor exerts a melanin production inhibitory action by containing the above-mentioned sugar-free pineapple extract as an active ingredient. The dermal papilla cell proliferation promoting agent exerts a dermal papilla cell proliferation promoting action by containing the aforementioned sugar-free pineapple extract as an active ingredient. The laminin-5 production promoting agent of the present invention promotes the formation of the basement membrane of the skin through the excellent laminin-5 production, for example, to prevent and improve the skin aging symptoms such as wrinkles and elasticity. Become a s 19 201225989 can. However, the laminin-5 production promoter of the present invention can be used for all uses having the meaning of promoting the production of laminin-5 in addition to these uses. The epidermis hyaluronic acid production promoter of the present invention promotes skin aging symptoms such as moisturizing and elastic properties through the promotion of an excellent skin hyaluronic acid, for example, to improve moisturizing and elastic properties, and to prevent and reduce rough skin, wrinkles, and elasticity. become possible. However, the epidermal hyaluronic acid production promoter of the present invention can be used for all purposes having a meaning of promoting the promotion of epidermal hyaluronic acid in addition to these uses. The hyaluronidase activity inhibitor of the present invention inhibits hyaluronic acid activity by, for example, inhibits decomposition of hyaluronic acid, promotes release of histamine, enhances moisturizing, prevents and improves inflammation or allergy. may. However, the hyaluronidase activity inhibitor of the present invention can be used for all purposes having a meaning of exhibiting a hyaluronidase activity inhibitory function in addition to these uses. The branamine transferase-1 production promoter of the present invention promotes the action of the excellent indoleamine transferase-1, for example, the barrier function of the skin epidermis, the disappearance of rough skin texture, prevention and improvement of elasticity The aging symptoms such as lowering are possible. However, the branamine transferase-1 production promoter of the present invention can be used for all purposes having a meaning of promoting the production of branamine transferase-1 in addition to these uses. With the melanin production inhibitor of the present invention, it is possible to suppress the skin by the excellent melanin, for example, to protect the skin from ultraviolet rays, prevent and improve the aging symptoms of skin blackening, spots, and the like. However, the melanin production inhibitor of the present invention e 20 201225989 can be used for all uses having the meaning of suppressing melanin production in addition to these uses. In the dermal papilla cell proliferation promoter of the present invention, it is possible to promote the proliferation of hairy epithelial cells, the differentiation of hair, the prevention, and the improvement of the bristles through the promotion of the proliferation of the dermal papilla cells. In addition to these uses, the dermal papilla cell proliferation accelerating agent of the present invention can be used for all uses having the meaning of promoting the proliferation of dermal papilla cells. The laminin-5 production promoter, the epidermal hyaluronic acid production promoter, the hyaluronidase activity inhibitor, the branamine transferase-1 production promoter, the melanin production inhibitor, and the dermal papilla cell proliferation of the present invention The accelerator has an excellent effect, and the extract obtained from the edible pineapple is excellent in safety, so it is suitable for blending with foods and drinks, external preparations for skin, and hair growth agents. Further, the laminin-5 production promoter, the epidermal hyaluronic acid production promoter, the hyaluronidase activity inhibitor, the branamine transferase-1 production promoter, the melanin production inhibitor, and the dermal papillae of the present invention Since the cell proliferation promoter has an excellent effect, it can be suitably used as a test for laminin-5, epidermal hyaluronic acid, hyaluronidase, branamine transferase-1, and dermal papilla cells. Drugs; reagents for the study of functions and diseases related to hyaluronidase, laminin-5, epidermal hyaluronic acid, branamine transferase-1, melanin, and dermal papilla cells. Further, the laminin-5 production promoter, the epidermal hyaluronic acid production promoter, the hyaluronidase activity inhibitor, the branamine transferase-1 production promoter, the melanin production inhibitor, and the dermal papillae of the present invention Cell proliferation promotion 21 201225989 The agent can be suitably applied to humans, but can be used for animals other than humans as long as it can exert its respective effects. (Food and beverage) The food and drink of the present invention contains the sugar-free pineapple extract of the present invention, a laminin-5 production promoter, a hyaluronic acid production promoter, a hyaluronidase activity inhibitor, and bran. At least one of a trypsin transferase-1 production promoter, a melanin production inhibitor, and a dermal papilla cell proliferation promoter, and further contains other components as necessary. In the above-mentioned food and beverage system, the above-mentioned food-free product containing the sugar-free pineapple extract may be blended into any food or drink that does not interfere with the activity, and may be a food or drink containing the sugar-free pear extract as a main component. Further, the food or drink may be the aforementioned pineapple extract which does not contain sugar. The above-mentioned food and drink products have few doubts about the harm to human health. In the ordinary social life, the so-called oral or digestive tract administration of the ingested person is not limited by the administrative division of food, medicine, medical supplies, etc. The distinction means, for example, a wide range including general foods, health foods, health functional foods, beauty foods, medical supplies, and pharmaceuticals which are orally ingested. Further, the food or drink of the present invention is processed into a shape such as a drink, a hard capsule, a soft capsule, or a granule by a form, and can be easily eaten and used, and may be used in a wide range. The foods and drinks are not particularly limited, and may be appropriately selected depending on the purpose, for example, beverages such as refreshing drinks, carbonated drinks, nutritional drinks, fruit drinks, and lactic acid drinks (including concentrates and adjustment powders of such drinks) ); ice-cold, ice-cream powder (sherbet), package 1 ice and other cold desserts; 22 201225989 soba noodles, oolong noodles, hollow noodles, Junzi skin, burnt skin, Chinese noodles, instant noodles, etc. Kinds of snacks such as sugar, candy, chewing gum, chocolate, ingots, dim sum, biscuits, pectin, jelly, cream, baked biscuits, bread, etc.; grilled crab, chain fish, clams, fish, sardines, shrimps , aquatic fish, squid, squid, snail, saury, squid, scallop, scallop, abalone, sea urchin, caviar, Japanese abalone and other aquatic products; fish plate, ham, sausage and other aquatic products · livestock processed food; processing Dairy products such as milk, fermented milk, salad oil, tempura oil, margarine, mayonnaise, shortening, whipping cream for foaming, dressing Oils and fats processed foods; sauces, barbecue sauces and other seasonings; curries, stews, parent-child porridge, porridge, muesli, Chinese sturgeon, pork chop, scorpio, squid, beef rice, Yutian, Ma Po Refort pouch foods such as tofu, burdock, minced meat, egg soup, egg omelet, Junzi, siu mai, burger, meatball, etc.; non-staple food (chives) such as salad and smash; Beauty and nutritional supplements; pharmaceuticals, pharmaceuticals, and other pharmaceuticals such as sharpeners, granules, capsules, beverages, troches, etc. Moreover, the above-mentioned foods and drinks are not limited to the above-mentioned examples. The above-mentioned food-free food-free pineapple extract, laminin-5 production promoter, epidermal hyaluronic acid production promoter, hyaluronidase activity inhibitor, and branamide transferase_1 production promotion The content of any one of the agent, the melanin production inhibitor, and the dermal papilla cell proliferation promoter differs depending on the food or drink to be added, and cannot be uniformly prescribed, but the suspects, capsules, and the like are opened. The amount of the extract of the phoenix extract containing no sugar is preferably 1% by mass to 90% by mass, and the other food and beverage products are changed to 23 201225989. 0 〇〇 1 mass 〇 / 〇 ~ % Mass % is preferred. In addition, it is preferable to adjust the intake amount of the pineapple extract containing no sugar per day to about 1 mg to about 10,000 mg per adult. The other components mentioned above can be exemplified by auxiliary materials, additives, and the like which are usually used in the production of the aforementioned foods and drinks. The auxiliary materials and additives are not particularly limited, and may be appropriately selected depending on the purpose. For example, glucose, fructose, vegetable sugar, maltose, sorbitol, stevi〇side, and catechin may be exemplified (for example). (6), corn sugar poly, lactose, citric acid, tartaric acid, malic acid, succinic acid, lactic acid, L-ascorbic acid, dl-a_tocopherol, isoascorbic acid = (― erythc^ate), glycerol, internal glycol, glycerol Fatty acid vinegar, polyglycerin fatty acid ester, sucrose fatty acid ester, s〇rbit (10) acid ester, arabic gum, carrageenan (Xie ag_n), complex protein ( Casein), gelatin, pectin ((4) (5), agar, vitamin B, niacin amide, calcium pantothenate, amino acids, calcium salts, pigments, spices, preservatives, etc. Ingestion by the mouth, by the action of the effective ingredient == pear extract, it can achieve the effect of the hyaluronic-hyperglyphic effect, the layer of egg-producing effect, and the production of sense transferase-1. Role, and the promotion of cell proliferation At least one of the products. The product of the month is suitable for humans, but as long as it has a different effect, it can also be applied to animals that are out of the way. 24 201225989 (External preparation for skin) The present invention The skin external preparation contains the sugar-free pineapple extract of the present invention, and a laminin_5 production promoter, epidermal hyaluronic acid production promoter, hyaluronidase activity inhibitor, and branamide transferase. Any one of the promoter, the melanin production inhibitor, and the dermal papilla cell proliferation promoter, and the other components are required to be contained therein. The skin external preparation means various agents for use on the skin. There is no particular limitation on the classification. For example, it includes a wide range of skin-based materials, medical supplies, pharmaceuticals, etc. The skin external preparations can be used to prevent the activity of the pineapple extract without sugar. In addition to the skin (4), it may be a skin external preparation containing the above-mentioned pineapple extract containing no sugar as a main component. The pineapple extract does not contain sugar. The skin external preparation is not particularly limited, and may be appropriately selected according to the purpose, for example, soft f, _, lotion, beauty lotion, lotion, mask, foundation, Lipstick, human bath, hair lotion, hair lotion, soap, shower gel, etc. 'The other ingredients mentioned above' are not particularly limited, and may be selected to produce a skin phase agent depending on the purpose. The ingredients usually used, for example, can be exemplified by _ 'kill 8, antibacterial agent, whitening agent, ultraviolet absorber, moisturizer, cell activator, anti-aging agent, anti-inflammatory anti-allergic agent, antioxidant, / tongue! Oxygen removal removes J 'steroids, soils, hydrocarbons, fatty acids, alcohols, esters, surfactants, perfumes, etc. In addition to the above-mentioned external skin cream Φ) 乂t ^, there is no sugar pineapple extract, 201225989 laminin-5 production promoter, epidermal hyaluronic acid production promoter, hyaluronidase activity inhibitor, bran The content of the indole transferase-1 production promoter, the melanin production inhibitor, and the dermal papilla cell proliferation promoter is not particularly limited, and may be appropriately selected depending on the type of the external preparation for skin, for example, The amount of the pineapple extract containing no sugar is preferably 0.0001% by mass to 10% by mass, more preferably 0.001% by mass to 5% by mass. (Hair growth agent) The hair growth agent of the present invention contains the sugar-free pineapple extract of the present invention, a laminin-5 production promoter, an epidermal hyaluronic acid production promoter, and a hyaluronidase activity inhibitor. A branamide transferase-1 production enhancer, a melanin production inhibitor, and a dermal papilla cell proliferation promoter, and may contain other components as necessary. The hair growth agent may be a hair growth agent containing the sugar-free pineapple extract as a main component, without interfering with the activity thereof, by blending the sugar-free pineapple extract with any hair growth agent. . Further, the hair growth agent may be the aforementioned pineapple extract containing no sugar. The hair growth agent of the present invention promotes laminin-5 production, promotes epidermal hyaluronic acid production, inhibits hyaluronidase activity, promotes the production of indoleamine transferase-1, and produces melanin. For example, it can promote the proliferation of the papillary cells and the hairpin epithelial cells, the differentiation of the hair, and the like, and can be suitably used for preventing and improving the finening and hair removal. In addition, the term "hair growth agent" as used in the present specification means that the hair 26 201225989 is used to: hair: J to maintain the health of the hair, prevent the tax hair, and the hair of the present invention, and also includes a "hair raising agent". Therefore, t growth Μ can also be preferably used as a hair growth agent. The hair growth agent for the skin has an excellent effect, and is suitable for use in a skin external preparation for blending hair, hair, and the like, which is suitable for "two-day use and safety". In the case of the administration site of the agent, for example, the head of the hair, the hair root of the hair, the sebaceous gland of the scalp, etc. is preferable. For the skin external application type, for example, an ointment, a cream, a lotion, Gel, sol) spray, solution, etc. For hair cosmetics, for example, hair tG (hairtGnk), hair liquid, shampoo, hair oil, moisturizing The above-mentioned non-sugar pineapple extract, laminin-5 production promoter, epidermal hyaluronic acid production promoter, hyaluronidase activity inhibitor, and indoleamine transfer are described in the hair growth agent. The content of the enzyme "promoting agent, the melanin production inhibitor, and the dermal papilla cell proliferation promoter" is not particularly limited, and may be appropriately selected depending on the type of the hair growth agent, etc. contain The amount of the pineapple extract is preferably 0.0001% by mass to 10% by mass, more preferably 1% by mass to 5% by mass. [Examples] Hereinafter, the present invention will be more specifically described by way of production examples and test examples, but the present invention is not limited thereto. (Production Example 1: Production of pineapple extract containing no sugar)

S 27 201225989 將lOOg鳳梨可食部之壓搾後殘渣（鳳梨肉質部）加入至1,000ml之90體積％的乙醇中，以回流萃& Π IL平取态於80。(：加熱萃取2小時，加熱時過濾。之後，使用蒸發器於減壓下濃縮，再進彳亍相同之過丨慮處理。於獲得的殘涪，以5 〇〇 1 之水洗淨，獲得去除糖質的糊狀之不含有糖之鳳 1.5g。萃取物之產率為1.5 (質量％)。於獲得的不含有糠之鳳梨萃取物進行如以下之成分分析。將前述不含有糖之鳳梨萃取物溶解於5〇體積％的乙醇成為樣品濃度2 0體積％者作為被驗試料使用，藉由葡萄糖 /果糖分析套組（F-套組（葡萄糖/果糖），j.K. Internati〇nal 股份有限公司製）測量葡萄糖及果糖之含量。測量結果得知前述不含有糖之鳳梨萃取物含有0.03質量％之葡萄糖、 0·01質量％之果糖。 · 將於上述之製造例1獲得的不含有糖之鳳梨萃取物作為被驗試料使用，如以下之試驗例1〜6所示，進行關於不含有糖之鳳梨萃取物的美肌、美白及毛髮生長的評價。又，作為對照鳳梨萃取物’使用將1〇〇%鳳梨果汁（市售鳳梨搾汁物）200ml ’使用蒸發器於減壓下濃縮，進行冷凍乾燥而獲得的26.3g之鳳梨搾汁冷凍乾燥物。於該鳳梨搾汁冷凍乾燥物0.413g/L，與前述不含有糖之鳳梨萃取物同樣地測量葡萄糖及果糖之含量的結果，得知該鳳梨搾汁冷束乾燥物含有29.1質量％之葡萄糖、26.6質量％之果糖。 (試驗例1 :層黏連蛋白-5產生促進作用試驗）S 27 201225989 The crushed residue (pineapple fleshy portion) of the lOOg pineapple edible portion was added to 1,000 ml of 90% by volume of ethanol, and refluxed & Π IL was taken at 80. (: The mixture was heated for 2 hours, and filtered while heating. Thereafter, it was concentrated under reduced pressure using an evaporator, and then subjected to the same treatment. The obtained residue was washed with 5 〇〇1 of water to obtain The saccharide-like paste was removed without the sugar phoenix 1.5 g. The yield of the extract was 1.5 (% by mass). The obtained pineapple extract containing no sputum was subjected to the following composition analysis. The pineapple extract was dissolved in 5 vol% of ethanol to a sample concentration of 20% by volume as the test sample, and the glucose/fructose analysis kit (F-set (glucose/fructose), jK Internati〇nal limited stock The content of glucose and fructose was measured by the company. The results showed that the pineapple extract containing no sugar contained 0.03 mass% of glucose and 0.101 mass% of fructose. The sugar obtained in the above-mentioned production example 1 did not contain sugar. The pineapple extract was used as a test sample, and the evaluation of the skin, whitening, and hair growth of the pineapple extract containing no sugar was carried out as shown in the following Test Examples 1 to 6. Further, as a control pineapple extract The extract was used to freeze the dried extract of 26.3 g of pineapple juice obtained by concentrating 1% by weight of pineapple juice (commercial pineapple juice) 200 ml 'concentrated under reduced pressure using an evaporator. The freeze-dried product was 0.413 g/L, and the content of glucose and fructose was measured in the same manner as the above-mentioned pineapple-free extract, and it was found that the pineapple juice cold-dried product contained 29.1% by mass of glucose and 26.6% by mass of fructose. (Test Example 1: Test for promoting adhesion of laminin-5)

S 28 201225989 <試驗方法> 將人類正常新生兒皮膚表皮角化細胞（NHEK，倉敷紡績股份有限公司製），使用人類正常新生兒表皮角化細胞用培養基（KGM，倉敷紡績股份有限公司製）來培養後，經由胰蛋白酶（trypsin)處理將細胞回收。以無添加ΒΡΕ之 KGM ( KGM-BPE ’倉敷紡績股份有限公司製）稀釋回收的細胞成為1X105個細胞/mL之濃度後，於24孔盤中每1孔各接種500pL ’培養2日。培養終了後，取出培養基，於各孔中添加500μΙ^以KGM-ΒΡΕ溶解的被驗試料，培養48 小時。培養終了後，將上清液1〇〇jliL移至ELISA平盤，於 37C2小時使吸附於平盤後，去除溶液，以含〇〇5體積％之Tween 20的填酸生理緩衝液（〇〇5%pBS T，阳7 4)進行洗淨。之後，以含1體積％牛血清白蛋白的磷酸生理緩衝液 (ρΗ7·4 )，進行封阻（blocking )操作。去除溶液，以 0.05%PBS-T進行洗淨，使與抗人層黏連蛋白_5抗體（小鼠 IgG，日本h η股份有限公司製）反應。去除溶液，= 0.05%PBS-T進行洗淨，進行呈色反應。由獲得的測量結果，自下述式异出層黏連蛋白產生促進率（％)。層黏連蛋白-5產生促進作用之計算方法如处於表1。、、、。果示層黏連蛋白-5產生促進率（％)=人/；8><1〇〇· · · [惟，前述式（1)中，A、B各別表示 "1) A :被驗試料添加時之波長4〇5nm中的吸光度 29 201225989 B :無添加被驗試料時（對照組）之波長4〇5nm中的吸光度]。 [表1] 濃度層黏連蛋白-5產生促進率(〇/〇) (pg/mL) 不含有糖之鳳梨萃取物 --—— 鳳梨榨汁冷凍乾燥物 2.5 114.3±3.3“ 91.3±5.8 10 121.3±3.0** 88.7±2.2 平均士S,E.，n=4，**:p<〇.01 由表1之結果，認為不含有糖之鳳梨萃取物有濃度依存的層黏連蛋白-5產生促進作用。 (試驗例2 :表皮透明質酸產生促進作用試驗） <試驗方法> 將人類正常新生兒皮膚表皮角化細胞（NHEK)使用人類正常新生兒表皮角化細胞用培養基（KGM)培養後，經由胰蛋白酶處理將細胞回收。回收的細胞以KGM稀釋為 1x105個細胞/mL之濃度後，於96孔盤中每1孔各接種 10(^L ’培養24小時。培養終了後，添加100μ[以Kgm 溶解的被驗試料於各孔中，培養7日。培養後，各孔之培養基中的透明質酸量使用透明質酸結合蛋白質（HABP，生化學Biobusiness股份有限公司製）的三明治法加以測量。透明質酸產生促進作用之計算方法如下。結果示於表 2 ° 每1孔之透明質酸產生促進率（％)=Α/Βχ1〇〇· · · 式（2) 30 201225989 濃度表皮透明f -----------_____ !酸產生促進率(％) (pg/mL) 不含有糖之鳳梨萃取物汁冷凍乾燥物 25 108.5±1.3** 78.8 士 4-4 100 149.7 土 2.1 … —--_ ______ 79.6±4.6 平均士S.E_，n=5，J^<001，*“:p<〇〇〇1 [惟，前述式（2)中，A、B表示 A:被驗試料添加時之透明質酸量 B :無添加被驗試料時之透明質酸量]。 [表 2] _ 由表2之結果，認為不含有糖之鳳梨萃取物具有意義的表皮透明質酸產生促進作用。 (試驗例3 :透明質酸酶活性抑制作用試驗） <試驗方法〉於0.2mL溶解被驗試料的〇.imo丨乙酸缓衝液 (pH3.5)中添加O.lmL透明質酸酶溶液（TypeIV_s (來自牛睪丸）、400 NF 單位/mL ; Sigma-Aldrich Japan 製），於 37 C反應20分鐘。再者，添加〇 2mL作為活性化劑之 2.5mmol/；L氯化鈣’於37°C反應20分鐘。於其中添加0.5mL 之0.4mg/mL透明質酸鈉溶液（來自禽雞冠的透明質酸鈉’ 和光純藥工業股份有限公司製），並於37〇c反應4〇分鐘。之後’添加〇.4mol/L氫氧化鈉〇 2mL，終止反應並冷卻後，於各反應溶液中添加硼酸溶液〇2mL，煮沸3分鐘。冰冷後添加P-DABA試藥（p_二甲基胺基苯甲醛，和光純藥工業版伤有限公司製）6mL，於37°C反應20分鐘。之後， 31 201225989 測量波長585nm中的吸氺疮 ^ . W及先度。以同樣方法進行空白試驗加以修正。透明質酸酶活性抑制作用之計算方法如下。結果示於表3 ° 透明質酸酶活性抑制率（％)= { 1- (St-Sb) / (Ct-Cb)} χίοο · · ·式⑶ [惟’前述式（3)巾，St、Sb、Ct及Cb各別表示 st ··被驗試料溶液之波長585nm中的吸光度 Sb :被驗試料溶液空白組之波長585nm中的吸光度 ct:對照組溶液之波長58Snm t的吸光度S 28 201225989 <Test method> Human normal neonatal skin epidermal keratinocytes (NHEK, manufactured by Kurabo Industries, Ltd.), using human normal neonatal epidermal keratinocyte culture medium (KGM, manufactured by Kurabo Industries, Ltd.) After the culture, the cells were recovered via trypsin treatment. The cells diluted and purified by KGM (KGM-BPE KK) were added to a concentration of 1×10 5 cells/mL, and then inoculated at 500 pL for 2 days in each well of a 24-well plate. After the completion of the culture, the medium was taken out, and 500 μM of the test sample dissolved in KGM-ΒΡΕ was added to each well, and cultured for 48 hours. After the end of the culture, the supernatant 1 〇〇jliL was transferred to an ELISA plate, and after adsorption to a flat plate at 37 ° C for 2 hours, the solution was removed to contain a physiological buffer containing 5% by volume of Tween 20 (〇〇 5% pBS T, yang 7 4) Washed. Thereafter, a blocking operation was carried out using a physiological phosphate buffer (ρΗ7·4 ) containing 1% by volume of bovine serum albumin. The solution was removed and washed with 0.05% PBS-T to react with an anti-human laminin-5 antibody (mouse IgG, manufactured by Nippon HN Co., Ltd.). The solution was removed, and 0.05% PBS-T was washed to carry out a color reaction. From the obtained measurement results, the laminin production promotion rate (%) was derived from the following formula. The calculation method for the enhancement of laminin-5 production is shown in Table 1. , ,,. Fruit layer laminin-5 production promotion rate (%) = human /; 8 > 1 〇〇 · · · [However, in the above formula (1), A and B respectively represent "1) A : Absorbance at a wavelength of 4 〇 5 nm when the test sample was added 29 201225989 B : Absorbance at a wavelength of 4 〇 5 nm when no test sample was added (control group). [Table 1] Concentration layer of laminin-5 production promotion rate (〇 / 〇) (pg / mL) Pineapple extract without sugar --- pineapple juice lyophilis 2.5 114.3 ± 3.3" 91.3 ± 5.8 10 121.3±3.0** 88.7±2.2 Average S, E., n=4, **: p<〇.01 From the results of Table 1, it is considered that the extract containing no sugar has a concentration-dependent laminin- (5) Test Example 2: Test for promotion of epidermal hyaluronic acid production <Test method> Human normal neonatal skin epidermal keratinocytes (NHEK) were cultured using human normal neonatal epidermal keratinocytes ( After KGM) culture, the cells were recovered by trypsin treatment. The recovered cells were diluted to a concentration of 1×105 cells/mL with KGM, and then inoculated for 10 hours in each well of a 96-well plate for 24 hours. Then, 100 μ [samples dissolved in Kgm were added to each well and cultured for 7 days. After the culture, the amount of hyaluronic acid in the medium of each well was hyaluronic acid-binding protein (HABP, manufactured by Biochemical Biobusiness Co., Ltd.). The sandwich method is measured. Hyaluronic acid production promotes The calculation method is as follows. The results are shown in Table 2 ° Hyaluronic acid production promotion rate per % (%) = Α / Βχ 1 〇〇 · · · (2) 30 201225989 Concentration epidermal transparency f ------ -----_____ ! Acid production promotion rate (%) (pg/mL) Pineapple extract juice without sugar lyophilized product 25 108.5±1.3** 78.8 士-4-4 100 149.7 地2.1...——--_ ______ 79.6±4.6 Average S.E_, n=5, J^<001,*":p<〇〇〇1 [However, in the above formula (2), A and B represent A: when the test sample is added The amount of hyaluronic acid B: the amount of hyaluronic acid in the case where the test sample was not added] [Table 2] _ As a result of Table 2, it is considered that the extract of the pineapple extract containing no sugar has a promoting effect on epidermal hyaluronic acid production. (Test Example 3: Hyaluronidase Activity Inhibition Test) <Test Method> O.lmL hyaluronidase solution was added to 0.2 mL of 〇.imo丨 acetic acid buffer (pH 3.5) in which the test sample was dissolved (pH 3.5) Type IV_s (from burdock), 400 NF units/mL; Sigma-Aldrich Japan), reacted at 37 C for 20 minutes. Further, add 2 mL of 〇2 mL as an activator to 2.5 mmol/L calcium chloride at 37 C for 20 minutes. To this was added 0.5mL of 0.4mg / mL sodium hyaluronate solution (sodium hyaluronic acid from rooster combs avian 'Wako Pure Chemical Industries, Ltd.), and reacted at 37〇c 4〇 minutes. Thereafter, 2 mL of 4. 4 mol/L sodium hydroxide hydrazine was added, and the reaction was terminated and cooled. Then, 2 mL of a boric acid solution 添加 was added to each reaction solution, and the mixture was boiled for 3 minutes. After ice-cooling, 6 mL of a P-DABA reagent (p-dimethylaminobenzaldehyde, manufactured by Wako Pure Chemical Industries, Ltd.) was added, and the reaction was carried out at 37 ° C for 20 minutes. After that, 31 201225989 measured the absorption of acne at a wavelength of 585 nm ^ W and the first degree. The blank test was performed in the same way and corrected. The calculation method for the inhibition of hyaluronidase activity is as follows. The results are shown in Table 3 ° Hyaluronidase activity inhibition rate (%) = { 1- (St-Sb) / (Ct-Cb)} χίοο · · · (3) [Only the above formula (3) towel, St, Sb, Ct, and Cb each indicate the absorbance at a wavelength of 585 nm of the sample solution Sb: the absorbance at a wavelength of 585 nm of the test sample solution blank group ct: the absorbance of the control solution at a wavelength of 58 Snm t

Cb .對照組溶液空白組之波長⑻⑽中的吸光度]。 __[* 3] 濃度 (Kg/mL) ------ 透明質酸酶活性抑制率Γ〇/(Λ 不含有糖之鳳梨萃取物鳳梨榨汁冷凍乾燥物 400 27.6 -22.6 ----- 由表3之結果認為不含有糖之鳳梨萃取物有透明質酸酶活性抑制作用。又，於鳳梨搾汁之乾燥物，相反地，認為有促進透明質酸酶活性的作用。 (試驗例4 :麵醯胺轉移酶-1產生促進作用試驗） <試驗方法> 將人類正常新生兒皮膚表皮角化細胞（NHEK)使用人類正常新生兒表皮角化細胞用培養基（KGM)培養後，經由胰蛋白酶處理將細胞回收。回收的細胞以KGM稀釋為 1x105個細胞/mL之濃度後，於96孔盤中每1孔各接種· 32 201225989 lOOpL，培養2日。培養終了後，添加1〇叫[以溶解的被f試料於各孔中.，培養24小時。培養後，除去培養基，使用單株抗人類麵醯胺轉移酶—丨抗體（Anti_Keratin〇cyteCb. Absorbance in the wavelength of the blank group of the control solution (8) (10)]. __[* 3] Concentration (Kg/mL) ------ Hyaluronidase activity inhibition rate Γ〇 / (Λ Pineapple extract without sugar, pineapple juice lyophilized product 400 27.6 -22.6 ---- - It is considered that the pineapple extract containing no sugar has an inhibitory effect on hyaluronidase activity as a result of Table 3. Further, the dried extract of the pineapple juice is conversely considered to have an effect of promoting hyaluronidase activity. 4: Test for promoting the production of indoleamine transferase-1) <Test method> After human normal neonatal skin epidermal keratinocytes (NHEK) were cultured using human normal neonatal epidermal keratinocyte culture medium (KGM), The cells were recovered by trypsin treatment, and the recovered cells were diluted to a concentration of 1×10 5 cells/mL with KGM, and then inoculated in a 96-well plate for 32 days at 25 201225989 lOOpL for 2 days. After the completion of the culture, 1 添加 was added. It is called [dissolved in each well in the well, and cultured for 24 hours. After the culture, the medium is removed, and a single anti-human face indole transferase-丨 antibody (Anti_Keratin〇cyte) is used.

Transglutaminase，Biomedical Technologies. Inc 製），藉由 ELIS A法來測量細胞固定於平盤的細胞表面上所表現的麩醯胺轉移酶-1之量。麩醯胺轉移酶-1產生促進作用之計算方法如下。結果示於表4。麩醯胺轉移酶-1產生促進率（％)=Α/Βχ1〇〇· · ·式 (4) " [惟，前述式（4 )中，A、Β各別表示 A .被驗试料添加時之波長4〇5nm中的吸光度 B :無添加被驗試料時（對照組）之波長4〇5nm中的吸光度]。 [表4] 濃度 (pg/mL) 麩醯胺轉移酶-1產生促進率(04) 不含有糖之鳳梨萃取物胤梨榨汁冷康乾燥物 50 122·2±2·8 … 99.5±1.〇 —- ---—ί^ί?ί.，η=6’ P<0.001 -------- 由表4之結果認為不含有糖之鳳梨萃取物具有意義的麩醯胺轉移酶-1產生促進作用。 (試驗例5 :黑色素產生抑制作用試驗） <試驗方法> 將B16黑色素瘤細胞使用含有10體積％1^8 (標準胎一 33 201225989 牛血清（STANDARD FETAL BOVINE SERUM)，HyClone 製）之 Dulbecco’s MEM ( Dulbecco’s modified Eagle 培養基 (1)，日水製藥股份有限公司製）培養後，經由胰蛋白酶處理將細胞回收。回收的細胞以含有10體積％FBS及 lmmol/L茶鹼（Theophylline，和光純藥工業股份有限公司製）的Dulbecco’s MEM稀釋為24.0x104個細胞/mL之濃度後’於48孔盤中每1孔各接種3〇(^L，培養6小時。培養終了後’將300pL以含有1〇體積灿奶及lmm〇i/L茶鹼的Dulbecco’s MEM溶解的被驗試料添加於各孔，培養4 曰。培養終了後’自各孔除去培養基，添加2mol/L之NaOH >谷液200pL，經由超音波破碎器將細胞破壞，測量於波長 475nm中的吸光度，作為黑色素產生量。又，為了測夏細胞生存率，同樣地於培養後，以4〇〇此Transglutaminase, manufactured by Biomedical Technologies. Inc., measures the amount of branamine transferase-1 expressed on the surface of cells fixed to a flat plate by the ELIS A method. The calculation method for the production of branamine transferase-1 is as follows. The results are shown in Table 4. Brassamine transferase-1 production promotion rate (%) = Α / Βχ 1 〇〇 · · · (4) " [However, in the above formula (4), A, Β each represents A. Test sample The absorbance at a wavelength of 4 〇 5 nm at the time of addition B: the absorbance at a wavelength of 4 〇 5 nm when the test sample was added (control group). [Table 4] Concentration (pg/mL) Brassamine transferase-1 production promotion rate (04) Pineapple extract containing no sugar, Avocado juice, cold-dried product 50 122·2±2·8 ... 99.5±1 .〇—- ---— ί^ί?ί., η=6' P<0.001 -------- The results of Table 4 are considered to be meaningless branamine transfer without the inclusion of sugar pineapple extract Enzyme-1 produces a promoting effect. (Test Example 5: Melanin production inhibition test) <Test method> Dulbecco's containing 10% by volume of 1^8 (Standard Fetal No. 33 201225989 bovine serum (STANDARD FETAL BOVINE SERUM), manufactured by HyClone) was used for B16 melanoma cells. After culturing with MEM (Dulbecco's modified Eagle medium (1), manufactured by Nissui Pharmaceutical Co., Ltd.), the cells were recovered by trypsin treatment. The recovered cells were diluted to a concentration of 24.0×10 4 cells/mL with Dulbecco's MEM containing 10% by volume of FBS and 1 mmol/L of theophylline (Theophylline, manufactured by Wako Pure Chemical Industries, Ltd.), and then per well of a 48-well plate. Each was inoculated 3 times (^L, cultured for 6 hours. After the completion of the culture), 300 pL of the test sample dissolved in Dulbecco's MEM containing 1 volume of milk and 1 mm〇i/L theophylline was added to each well, and cultured for 4 inches. After the completion of the culture, the medium was removed from each well, and 2 mol/L of NaOH > 200 g of the trough solution was added, and the cells were destroyed by an ultrasonic breaker, and the absorbance at a wavelength of 475 nm was measured as the amount of melanin production. Rate, similarly after cultivation, to 4

之PBS (-)磷酸生理緩衝液洗淨，於各孔中添加以終濃度 0.05mg/mL 溶解於含有 10 體積％FBS 的 Dulbecc〇，s MEM 的 13.8mmol/L 中性紅（neutraired) 2〇〇μ[。培養 2 $ 小時後，捨去中性紅溶液，於各孔中添加2〇〇jiL之乙醇•乙酸溶液（乙醇：乙酸：水=50 : i : 49)，萃取色素。萃取後，測量於波長540nm中的吸光度。作為空白試驗，以同樣之方法試驗僅含有1〇體積 %FBS及lmmol/L茶鹼的Dulbecc〇，sMEM培養的細胞。黑色素產生抑㈣狀計算方法如下。結果示於表5。黑色素產生抑制率（％ )=The PBS (-) phosphate physiological buffer was washed, and added to each well at a final concentration of 0.05 mg/mL in Dulbecc® containing 10% by volume of FBS, and 13.8 mmol/L of neutral red (neutraired) in s MEM. 〇μ[. After 2 hours of incubation, the neutral red solution was discarded, and 2 〇〇之 of ethanol/acetic acid solution (ethanol: acetic acid: water = 50: i: 49) was added to each well to extract the pigment. After the extraction, the absorbance at a wavelength of 540 nm was measured. As a blank test, Dulbecc(R), sMEM cultured cells containing only 1% by volume of FBS and 1 mmol/L of theophylline were tested in the same manner. The calculation method of melanin production (tetra) is as follows. The results are shown in Table 5. Melanin production inhibition rate (%) =

{ 1- (B/D) / (A/C)} xlOO . . . -vw.N 34 201225989 [惟’前述式（5)中，A、B、C及D各別表示 A :未添加被驗試料的細胞於波長475nm中的吸光产 B .添加被驗试料的細胞於波長475nm中的吸光声 C .未添加被驗試料的細胞於波長54〇nm中的吸光产 D :添加被驗試料的細胞於波長54〇nm中的吸光度]。 [表 5] ^ 濃度 S---- 黑色素產生抑制率(%) (pg/mL) 不含有糖之鳳梨萃取物 ----- 鳳梨榨汁冷凍乾燥物 12.5 14.2±1.4 —-~. 6.5±0.5 25 13.8±1.3 ---^_ 8.8+4.6 50 20.4±1.6 11.6±1.8 100 23.0±1.6 ' ----- 15.4±2.8 由表5之結果，認為不含有糖之鳳梨萃取物較成為對照的鳳梨搾汁冷凍乾燥物有更強、且濃度依存的黑色素產生抑制作用。 (試驗例6:毛乳頭細胞增殖促進作用試驗） <試驗方法> 將正常人類頭髮毛乳頭細胞（東洋紡績股份有限公司製）使用毛乳頭細胞增殖培養基（東洋紡績股份有限公司製）培養後’經由胰蛋白酶處理將細胞回收。回收的細胞使用含有 10 體積°/〇FBS 的 Dulbecco’s MEM (Dulbecco，s modified Eagle培養基（1)，曰水製藥股份有限公司製）稀釋為1.0x104個細胞/mL之濃度後，於塗布膠原纖維的96 孔平盤中每孔接種200μΙ^ ’並培養3日。培養後，移除培s 35 201225989 養基’於各孔中添加溶解於無灰冑dmem (Duibecc〇，s modified Ε_培養基⑴，日水製藥股份有限公司製）的被驗試料2_L’再培養4日。錢頭細胞增_用係使用MTT分析來測量。培養終了後，移除培養基，於各孔中添加以終濃度G.4mg/mL之溶解於無血清之dmem的演化 3- (4,5·二曱基H2·基）·2,5·:苯基四錢（MTT，同仁化學研究所股份有限公司製）1〇〇μ[。培養2小時後，以 2-丙醇ΙΟΟμί萃取於細胞内生成的藍色曱月替 (formazan)。萃取後，測量於波長57〇nm的吸光度 (OD570)。同時測量作為濁度之波長65〇nm中的吸光度 (OD650 )，取兩者的差作為藍色曱月替生成量。毛乳頭細胞增殖促進作用之計算方法如下。結果示於表6。毛乳頭細胞增殖促進率（％) =Α/Βχ100 · · ·式（6) [惟，前述式（6)中，Α及Β分別表示 A :被驗試料添加時之OD570-OD650 B :無添加被驗試.料時之OD570-OD650]。濃度 (pg/mL) 毛乳頭細胞增殖促進率(°/〇) 不含有糖之鳳梨萃取物鳳梨榨汁冷凍乾燥物 25 114.3 土 U*** 99.0±1.8 平均土S.E,，n=6,…:p< 0.001 由表6之結果’確認僅不含有糖之鳳梨萃取物具有意義的毛乳頭細胞增殖促進作用。 [表 6] _ 36 201225989 產業可利用性本發明之不含有糖之鳳梨萃取物、層黏連蛋白-5產生促進劑、表皮透明質酸產生促進劑、透明質酸酶活性抑制劑、麩醯胺轉移酶-1產生促進劑、黑色素產生抑制劑、及毛乳頭細胞增殖促進劑因安全性及生產性優異且可日常攝取，且為便宜，同時具有優異的層黏連蛋白-5產生促進作用、表皮透明質酸產生促進作用、透明質酸酶活性抑制作用、麩醯胺轉移酶-1產生促進作用、黑色素產生抑制作用、及毛乳頭細胞增殖促進作用之至少任一種作用，故可摻合於美容用飲食品、摻合於皮膚外用劑、及摻合於毛髮生長劑、可適合利用作為研究用之試藥。【圖式簡單説明】 _飯〇【主要元件符號説明】無。{ 1- (B/D) / (A/C)} xlOO . . . -vw.N 34 201225989 [Only in the above formula (5), A, B, C and D each represent A: not added The absorbance of the cells of the test sample at a wavelength of 475 nm B. The absorbance of the cells of the test sample at a wavelength of 475 nm. The absorbance of the cells without the test sample at a wavelength of 54 〇 nm: Addition test The absorbance of the sample cells at a wavelength of 54 〇 nm]. [Table 5] ^ Concentration S---- Melanin production inhibition rate (%) (pg/mL) Pineapple extract without sugar----- Pineapple juice lyophilis 12.5 14.2±1.4 —-~. 6.5 ±0.5 25 13.8±1.3 ---^_ 8.8+4.6 50 20.4±1.6 11.6±1.8 100 23.0±1.6 ' ----- 15.4±2.8 From the results of Table 5, it is considered that the pineapple extract containing no sugar is more The control pineapple juice lyophilized product has a stronger, concentration-dependent melanin production inhibitory effect. (Test Example 6: Test for promoting the proliferation of dermal papilla cells) <Test method> After normal human hair dermal papilla cells (manufactured by Toyobo Co., Ltd.) were cultured using dermal papilla cell proliferation medium (manufactured by Toyobo Co., Ltd.) 'Recover cells by trypsin treatment. The recovered cells were diluted to a concentration of 1.0×10 4 cells/mL using Dulbecco's MEM (Dulbecco, s modified Eagle medium (1), manufactured by Surabaya Pharmaceutical Co., Ltd.) containing 10 volumes of 〇/〇FBS, and coated with collagen fibers. Each well of the 96-well plate was inoculated with 200 μM ^ ' and cultured for 3 days. After the culture, the cultured sample 2_L' was re-cultured in the wells and added to the ash-free dmem (Duibecc®, s modified Ε_media (1), manufactured by Risui Pharmaceutical Co., Ltd.). 4th. The growth of the head cells was measured using MTT assay. After the end of the culture, the medium was removed, and the evolution of the serum-free dmem at a final concentration of G.4 mg/mL was added to each well 3-(4,5·didecyl H2·yl)·2,5·: Phenyl four money (MTT, manufactured by Tongren Chemical Research Institute Co., Ltd.) 1〇〇μ[. After 2 hours of incubation, the blue formazan produced in the cells was extracted with 2-propanol ΙΟΟμί. After the extraction, the absorbance at a wavelength of 57 〇 nm (OD570) was measured. At the same time, the absorbance (OD650) in the wavelength of 65 〇 nm as the turbidity was measured, and the difference between the two was taken as the amount of blue sputum generation. The calculation method for promoting the proliferation of dermal papilla cells is as follows. The results are shown in Table 6. Proliferation rate of dermal papilla cells (%) = Α / Βχ 100 · · · (6) [However, in the above formula (6), Α and Β respectively represent A: OD570-OD650 when added to the test sample B: no addition Tested. OD570-OD650]. Concentration (pg/mL) Proliferation rate of dermal papilla cells (°/〇) Pineapple extract without sugar, pineapple juice, lyophilized product 25 114.3 Soil U*** 99.0±1.8 Average soil SE,, n=6,... : p < 0.001 From the results of Table 6 'confirmation of the dermal papilla cell proliferation promoting effect which is only containing no sugar pineapple extract. [Table 6] _ 36 201225989 INDUSTRIAL APPLICABILITY The sugar-free pineapple extract, laminin-5 production promoter, epidermal hyaluronic acid production promoter, hyaluronidase activity inhibitor, and bran of the present invention Amine transferase-1 production promoter, melanin production inhibitor, and dermal papilla cell proliferation promoter are excellent in safety and productivity, and can be taken daily, and are inexpensive, and have excellent laminin-5 production promoting action. And at least one of hyaluronic acid production promoting action, hyaluronidase activity inhibiting action, branamine transferase-1 production promoting action, melanin production inhibiting action, and dermal papilla cell proliferation promoting action, so that it can be blended It is suitable for use as a test drug for research, for use in cosmetic foods, incorporation into skin external preparations, and incorporation into hair growth agents. [Simple description of the diagram] _ rice cooker [Description of main component symbols] None.

S 37S 37

Claims

201225989 VII. Patent application scope: 1.- Obtained from the edible part of the pineapple extract without sugar, characterized by the extraction of pineapple by solvent. Please use the third and third patents to contain glucose and fructose. It is a solvent-free extract of pineapple extract containing no sugar, and the patent range is as follows, wherein the solvent is 70% by volume~, (10) the sugary pineapple extract of the carrier is 4. The pineapple extract without sugar is extracted. Fang: It: drunk aqueous solution. The extraction of the edible portion of the pineapple, the removal of glucose is characterized by the dissolution of 5. According to the fourth method of the patent application, the method of extracting 2 pineapple extract by solvent is as follows. Or the remnants of the 5 items of the 2 & A manufacturing method in which the solvent is a solution containing no sugar and a pineapple extract. Accumulation / 〇 ~ 100 volume 0 / 〇 ethanol water 7. - Laminin-5 production promoter, the scope of the first or second item does not contain: characterized by the application of a kind of epidermal hyaluronic acid production promotion Agent, ^f extract. 9 Scope No. 1 or 2 is not included, the temple sign contains the patent application - a hyaluronidase activity inhibitor, t = pear extract. The scope of the article does not contain, for example, the patent application. A nocilon transferase-1 production promoter,: take. In the first or second aspect of the patent range, it is characterized in that it contains a pineapple extract as described in the application for a melanin production inhibitor. The first or second item does not contain the sugar eve: 2, and the patent scope is < the pineapple extract 11. 201225989 12. The dermal papilla cell proliferation promoter is characterized by containing the first or second patent application scope. The pineapple extract containing no sugar is described. A food or drink comprising a pineapple extract containing no sugar as described in claim 1 or 2. A skin external preparation comprising a pineapple extract containing no sugar as described in claim 1 or 2. A hair growth agent comprising a pineapple extract containing no sugar as described in claim 1 or 2. 39 201225989 IV. Designated representative map: (1) The representative representative of the case is: None. (2) A brief description of the component symbols of this representative figure: None. 5. If there is a chemical formula in this case, please disclose the chemical formula that best shows the characteristics of the invention: