KR20180037163A - Sugar-free pineapple extract, method for producing the extract, and application of the extract - Google Patents

Abstract

The present invention relates to a sugar-free pineapple extract which is obtained by extracting edible pineapple using a solvent and does not contain glucose and fructose. According to the present invention, it is possible to secure stability and productivity.

Description

SUGAR-FREE PINEAPPLE EXTRACT, METHOD FOR PRODUCING THE EXTRACT, AND APPLICATION OF THE EXTRACT < RTI ID = 0.0 >

The present invention relates to a sugar-free pineapple extract, a method for producing the same, and a method for producing a laminin-5 production accelerator, a skin hyaluronic acid production promoter, a hyaluronidase activity inhibitor, a transglutaminase-1 production promoter, a melanin production inhibitor, Accelerators, food and drinks, external skin preparations and hair restorers.

The skin consists of epidermis, basement membrane and dermis. The dermis is composed of fibroblasts, extracellular matrix such as collagen (collagen fiber), elastin (elastic fiber) and hyaluronic acid secreted from these cells. The basement membrane is present at the epidermis and dermis boundary and not only connects the epidermis and the dermis, but also plays an important role in the maintenance of skin function (Marinkovich MP et al., J. Cell. Bio 1. 1992 199: 695-703) . In the young skin, the interaction of these skin tissues maintains homeostasis, thereby securing water retention, flexibility, and elasticity. As a result, the skin is not taut, glossy and remains moist.

However, when affected by external factors such as ultraviolet rays, remarkable air drying, excessive skin washing, stress or smoking, or aging, collagen degradation or deterioration, which is a major component of the extracellular matrix, (Lavker et al., J. Invest. Dermatol., 1979 73: 59-66). In addition, laminin-5 is a major constituent of the basement membrane. In addition, the production of hyaluronic acid, which is a natural moisturizing factor, is lowered. As a result, the moisturizing function and elasticity of the skin are lowered, abnormal exfoliation of the keratin is caused, and aging symptoms such as wrinkles, dullness, loss of skin texture and elasticity are exhibited.

Thus, by accelerating the production of laminin-5 and the production of hyaluronic acid, aging of the skin can be prevented or improved. In view of this, it is required to obtain a substance having a laminin-5 production promoting action and a hyaluronic acid production promoting action from a highly safe natural product.

Examples of those having laminin-5 production promoting action so far include, for example, Puerariae Radix extract, Licorice root extract or its flavonoid fraction, Anabolic extract, Pulai extract, Tinospora extract, Fenugreek extract, (Japanese Unexamined Patent Application Publication No. 2003-137768) and soybean extract (Japanese Unexamined Patent Publication (Kokai) No. 2004-217618), etc. have been reported in the past, such as Onnis extract, Merriot extract, host extract, have. In addition, attempts have been made to extract substances having hyaluronic acid production promoting action from natural products (see " fragrance journal ", 1992, No. 11, p43).

On the other hand, inflammatory diseases such as contact dermatitis, psoriasis, psittacian smallpox, various skin diseases accompanied by skin roughness, and the causes and manifestations are various colors. It is known that the cause is hyaluronidase activity.

Hyaluronate having affinity to body tissues is decomposed by ultraviolet rays and enzymes in the water system, and the maintenance effect is also reduced as the molecular weight is lowered. In addition, hyaluronic acid exists as an intercellular tissue and is also involved in vascular permeability. It is also believed that hyaluronidase is involved in degranulation from mast cells by activity in mast cells. Therefore, by inhibiting the activity of hyaluronidase, the hydrolytic enzyme of hyaluronic acid, it is possible to stabilize hyaluronic acid, prevent the release of chemical mediators such as histamine from mast cells, enhance moisturization and antifouling .

Examples of the herbal medicine having hyaluronidase activity inhibiting activity include extracts of Osbeksy subspecies (Japanese Patent Laid-Open Publication No. 2003-055242), Fuji tea extract (Japanese Patent Application Laid-Open No. 2003-012532) Rosemary extract, time extract, and melissa extract (see JP-A-8-333267).

In addition, the epidermis has a defensive function of protecting the skin from various stimuli from the outside world by making new keratinocytes always by cleavage and subsequent differentiation of keratinocytes. Particularly, in the differentiation process of keratinocytes, proteins such as phosphorylcholine are expressed from the porous layer to the granule layer, cross-linked by the action of the enzyme transglutaminase-1, and the insoluble cell membrane- (Corneal envelope; CE), which contributes to the stability of the cytoskeleton and structure of keratinocytes.

However, if the production of transglutaminase-1 in the epidermis is decreased due to various factors, formation of keratin-free thick film (CE) becomes incomplete and keratinization will not occur normally. As a result, the function of the keratin barrier and the moisturizing function of the skin are lowered, and the skin symptoms such as skin roughness and dry skin are considered to be exhibited.

In this respect, by increasing the production of transglutaminase-1 in the epidermis of keratinocytes and promoting the formation of keratin-free thick films to normalize the keratinization, degradation of the skin barrier function due to external stimuli such as drying or ultraviolet rays It is considered that various skin symptoms such as drying of the skin and roughness of the skin can be prevented or improved.

In the skin, melanin also protects the body from ultraviolet rays, but overproduction or uneven accumulation causes skin darkening or stain. In general, melanin is transformed from tyrosine by tyrosine by the action of enzyme tyrosinase biosynthesized in the pigment cells, and then from the waveguide to dopakinone, and then through intermediates such as 5,6-dihydroxyindophenol. Therefore, in order to prevent or ameliorate the dark color of the skin (skin pigmentosis), that is, for whitening, it is considered effective to inhibit the production of melanin, or to bleach already produced melanin. In this background, development of a substance that inhibits melanin production and leads to a whitening effect is actively performed.

Growth of hair grows and drops repeatedly according to periodic hair cycle (mother cycle) consisting of growing period, regenerating period, and resting period. In this hair cycle, a stage in which new blanks are formed from the resting stage to the growing stage is considered to be most important for hair growth. It is believed that the papilla cells play an important role in the proliferation and differentiation of the boll epithelial cells in this stage. The dermal papilla cells are located inside the hair follicle epithelium cells, which are composed of matrix cells and outer root cells in the vicinity of the hair follicles. They are located in the base part of the hair follicle wrapped around the basement membrane. They act on the hair follicle epithelial cells to help their proliferation , And plays an important role in hair differentiation ('Trends Genet', 1992, Vol.8, p. 56-61).

On the other hand, a skin external preparation containing a pineapple fruit juice extract or pineapple fruit extract as a whitening and skin improving ingredient has been reported (see Japanese Patent Application Laid-Open No. 2002-275081).

However, it can be consumed on a daily basis because of its excellent safety and productivity, and at the same time, it is inexpensive and has excellent laminin-5 production promoting action, epidermal hyaluronic acid production promoting action, hyaluronidase activity inhibiting action, transglutaminase- A melanin production-inhibiting action, and a dermal papilla cell proliferation-stimulating action, is very strong, but a satisfactory one is not yet provided.

SUMMARY OF THE INVENTION The present invention has been made to solve the above-mentioned conventional problems and to achieve the following objects. That is, the present invention relates to a pharmaceutical composition which is excellent in safety and productivity and can be routinely consumed, and at the same time, it is inexpensive and has excellent laminin-5 production promoting action, epidermal hyaluronic acid production promoting action, hyaluronidase inhibiting action, transglutaminase- 1 production promoting action, a melanin production inhibiting action, and a papilla cell proliferation promoting action, and a process for producing the same.

It is another object of the present invention to provide a laminin-5 production promoter having an excellent laminin-5 production promoting action and a high safety. It is another object of the present invention to provide an accelerator for production of epidermal hyaluronic acid having a superior skin hyaluronic acid production promoting action and a high safety. It is another object of the present invention to provide a highly safe hyaluronidase activity inhibitor having an excellent hyaluronidase activity inhibiting action. It is another object of the present invention to provide a transglutaminase-1 production promoter having an excellent transglutaminase-1 production promoting action and a high safety. It is another object of the present invention to provide a melanin-producing inhibitor having a superior melanin production inhibitory action and a high safety. It is another object of the present invention to provide an agent for promoting proliferation of a dermal papilla cell having an excellent dermal papilla cell proliferation action and a high safety. It is another object of the present invention to provide a food and drink, an external preparation for skin and a hair restorer, which contain the sugar-free pineapple extract.

In order to solve the above problems, the inventors of the present invention have conducted extensive research and have found the following findings. Namely, the pineapple extract obtained by extracting the edible part of pineapple with a solvent has excellent laminin-5 production promoting action, epidermal hyaluronic acid production promoting action, hyaluronidase activity inhibiting action, transglutaminase-1 production promoting action, melanin Production inhibitory action and promoting the proliferation of the dermal papilla cell, the pineapple extract is effective for the production of laminin-5 production promoter, epidermal hyaluronic acid production promoter, hyaluronidase activity inhibitor, transglutaminase-1 production promoter, melanin production Inhibitors and dermal papilla cell proliferation accelerators, and as food and beverages, external dermatological preparations and hair restorers.

The present invention is based on the above knowledge by the present inventors, and means for solving the above problems are as follows. In other words,

(1) A sugar-free pineapple extract, which is obtained by extracting a pineapple edible portion with a solvent, and does not contain glucose and fructose.

(2) The sugar-free pineapple extract according to the above (1), which is obtained by extracting the residue after the compression of the pineapple edible portion with a solvent.

(3) The sugar-free pineapple extract according to the above (1) or (2), wherein the solvent is an aqueous solution of 70 volume% -100 volume% ethanol.

(4) A method for producing a sugar-free pineapple extract, which comprises extracting a pineapple edible portion with a solvent to remove glucose and fructose.

(5) The method for producing a sugar-free pineapple extract according to (4), wherein the residue after the compression of the pineapple edible portion is extracted with a solvent.

(6) The method for producing a sugar-free pineapple extract according to (4) or (5), wherein the solvent is an aqueous solution of 70 volume% -100 volume% ethanol.

(7) A laminin-5 production promoter comprising the sugar-free pineapple extract according to any one of (1) to (3) above.

(8) The epidermal hyaluronic acid production promoter according to any one of (1) to (3), which contains the sugar-free pineapple extract.

(9) A hyaluronidase activity inhibitor characterized by containing a saccharide-free pineapple extract according to any one of (1) to (3) above.

(10) A transglutaminase-1 production promoter comprising the sugar-free pineapple extract according to any one of (1) to (3) above.

(11) A melanin production inhibitor characterized by containing a saccharide-free pineapple extract according to any one of (1) to (3) above.

(12) A papilla cell proliferation promoter which comprises the sugar-free pineapple extract according to any one of (1) to (3) above.

(13) A food or drink containing the sugar-free pineapple extract according to any one of (1) to (3).

(14) An external preparation for skin, which comprises the sugar-free pineapple extract according to any one of (1) to (3) above.

(15) A hair restorer comprising the sugar-free pineapple extract according to any one of (1) to (3) above.

According to the saccharide-free pineapple extract of the present invention and its preparation method, it is possible to obtain daily consumption with excellent safety and productivity, and at the same time to provide an excellent laminin-5 production promoting action, a skin hyaluronic acid production promoting action, a hyaluronidase Containing pineapple extract having at least any one of an activity inhibiting action, a transglutaminase-1 production promoting action, a melanin production inhibiting action, and a dermal papilla cell proliferation promoting action, and a process for producing the same.

According to the laminin-5 production promoter of the present invention, it is possible to provide a laminin-5 production promoter having excellent laminin-5 production promoting action and high safety which can solve many conventional problems.

INDUSTRIAL APPLICABILITY According to the epidermal hyaluronic acid production promoter of the present invention, it is possible to provide an accelerator for production of epidermal hyaluronic acid having a superior action of promoting epidermal hyaluronic acid production and capable of solving many conventional problems and having high safety.

According to the hyaluronidase activity inhibitor of the present invention, it is possible to provide a hyaluronidase activity inhibitor having excellent hyaluronidase activity inhibiting action and capable of solving many conventional problems and having high safety.

According to the transglutaminase-1 production promoter of the present invention, there is provided a transglutaminase-1 production promoter having excellent transglutaminase-1 production promoting action and capable of solving many conventional problems, Can be provided.

According to the melanin production inhibitor of the present invention, it is possible to provide a melanin production inhibitor having excellent melanin production inhibiting action and high safety, which can solve many conventional problems.

According to the dermal papilla cell growth promoter of the present invention, it is possible to provide a dermal papilla cell growth promoter having excellent dermal papilla cell proliferation action and high safety, which can solve many conventional problems.

(pineapple)

Pineapple (Pinapple) belongs to the genus Pineapple and Pineapple, the scientific name of the plant: Ananas comosus (L.) Merr. Ananas sativus Schult, also known as Fengli in China. The fruits are large-angled, fleshy, yellow-ripened and emit fragrance, and are used for food. The origin of pineapples is mainly produced in the United States, the Philippines, Malaysia, Brazil, Australia and the like. In obtaining the extract used in the present invention, the kind and origin of pineapple are not particularly limited.

(Method for producing sugar-free pineapple extract and sugar-free pineapple extract)

The sugar-free pineapple extract of the present invention is obtained by extracting a pineapple edible portion as an extraction raw material with a solvent. This sugar-free pineapple extract should not contain glucose and fructose.

The method for producing the sugar-free pineapple extract of the present invention includes extracting the pineapple edible portion with a solvent to remove glucose and fructose, and includes other processes appropriately selected as necessary.

As the raw material for extracting the sugar-free pineapple extract, it is possible to appropriately select it according to the purpose without limitation, as long as it is a pineapple edible part such as flesh and core (core). However, the residue after the compression of the pineapple edible part, Fibers (pineapple pulp) are particularly preferred.

It is preferable that the above-mentioned raw material for extraction is washed, dried, and pulverized after ingestion. Here, the drying may be performed in the sun or in a commonly used dryer.

The sugar-free pineapple extract can be easily obtained by adding the pineapple edible portion to the solvent and extracting the mixture at room temperature or below the boiling point of the solvent using an arbitrary apparatus.

The solvent may be appropriately selected according to the purpose without particular limitation, and examples thereof include lower aliphatic alcohols having 1 to 5 carbon atoms such as methanol, ethanol, propanol and isopropanol; Lower aliphatic ketones such as acetone and methyl ethyl ketone; Polyhydric alcohols having 2 to 5 carbon atoms such as 1, 3-butylene glycol, propylene glycol and glycerin; Hexane; And a mixed solvent of a hydrophilic organic solvent such as a lower aliphatic alcohol, a lower aliphatic ketone, or a polyhydric alcohol, and water. Of these, a mixed solvent of a hydrophilic organic solvent and water is preferable. The hydrophilic organic solvent is not particularly limited and may be appropriately selected according to the purpose. Preferred examples of the hydrophilic organic solvent include methanol, ethanol, propanol, 1, 3-butylene glycol, glycerin, propylene glycol and the like. Ethanol is more preferable.

When a lower alcohol is used as the hydrophilic organic solvent in a mixed solvent of water and a hydrophilic organic solvent, the content of the lower aliphatic alcohol in the mixed solvent is preferably 10 vol% to 100 vol%, more preferably 70 vol% , More preferably -100 vol%, and particularly preferably 90 vol%. When a lower fatty acid ketone is used as the hydrophilic organic solvent, the content of the lower aliphatic ketone in the mixed solvent is preferably 10% by volume to 80% by volume. When a polyhydric alcohol is used as the hydrophilic organic solvent, the content of the polyhydric alcohol in the mixed solvent is preferably 10% by volume to 90% by volume.

The method for extracting sugar-free pineapple extract is not particularly limited as long as it is possible to elute a lipid soluble component contained in the pineapple edible part in the solvent, and can be carried out according to a conventional method. It is not necessary to employ a special extraction method in the extraction process, and any device may be used under room temperature to reflux heating.

Specifically, as the method of extracting sugar-free pineapple extract, for example, the extraction raw material such as a residue (pineapple pulp) after pressing the pineapple portion is put into a treatment tank filled with the above-mentioned solvent such as an aqueous ethanol solution, , The mixture was heated and extracted with a reflux extractor at 80 ° C for 2 hours, and the resulting mixture was heated to elute a lipophilic component by heat filtration. The resulting mixture was concentrated under reduced pressure using an evaporator and then subjected to the same filtration treatment. Followed by washing to remove saccharides such as glucose and fructose to obtain an objective sugar-free pineapple extract.

At this time, the extraction conditions can be appropriately adjusted according to the extraction raw materials, etc., but the amount of the extraction solvent is preferably 5 times to 20 times (mass ratio) to the pineapple edible part as the extraction raw material, and the extraction time is 1 hour to 3 hours And the extraction temperature is preferably from room temperature to 95 ° C.

The obtained sugar-free pineapple extract is subjected to diluting, concentrating, drying, and purification treatments according to a conventional method to obtain a diluted product, concentrated product, dried product, crude purified product and purified product of the sugar-free pineapple extract .

In addition, the sugar-free pineapple extract thus obtained can be used as a laminin-5 production promoter, epidermal hyaluronic acid production promoter, hyaluronidase activity inhibitor, transglutaminase-1 production promoter, melanin production inhibitor, Any one of them may be used, but the concentrated liquid and the dried material are preferable in view of ease of use. In order to obtain the dried product, a carrier such as dextrin, cyclodextrin or the like may be added to improve hygroscopicity.

(Non-content component)

In the present invention, the term " sugar " of " sugar-free " means glucose and fructose. The term "not containing", "not containing sugar", "removing sugar" means that the content of each sugar in the sugar-free pineapple extract is 0.5 mass% or less, but 0.2 mass% or less is preferable Do.

The content of the glucose and fructose may be measured according to the kit attached protocol, for example, using a glucose / fructose assay kit (F-kit (glucose / fructose), manufactured by J. K. International). As a test sample, the sugar-free pineapple extract may be dissolved in an aqueous 50% by volume ethanol solution to a concentration of 5% by volume of the sample.

(Laminin-5 production promoter, epidermal hyaluronic acid production promoter, hyaluronidase activity inhibitor, transglutaminase-1 production promoter, melanin production inhibitor, and papillary cell proliferation promoter)

The laminin-5 production promoter of the present invention contains the above sugar-free pineapple extract and, if necessary, further contains other components.

The epidermal hyaluronic acid production promoter of the present invention contains the above sugar-free pineapple extract and, if necessary, further contains other ingredients.

The hyaluronidase activity inhibitor of the present invention contains the above sugar-free pineapple extract and, if necessary, further contains other components.

The transglutaminase-1 production promoter of the present invention contains the above sugar-free pineapple extract and, if necessary, further contains other components.

The melanin production inhibitor of the present invention contains the sugar-free pineapple extract and, if necessary, further contains other ingredients.

The dermal papilla cell growth promoting agent of the present invention contains the above sugar-free pineapple extract and, if necessary, further contains other components.

The laminin-5 production promoter of the present invention may be composed of only the sugar-free pineapple extract, or may contain other ingredients.

The content of the sugar-free pineapple extract in the laminin-5 production promoter is not particularly limited and may be appropriately selected depending on the purpose.

The epidermal hyaluronic acid production promoter of the present invention may be composed of only the sugar-free pineapple extract or may contain other ingredients.

The content of the sugar-free pineapple extract in the epidermal hyaluronic acid production promoting agent is not particularly limited and may be appropriately selected depending on the purpose.

The hyaluronidase activity inhibitor of the present invention may be composed of only the sugar-free pineapple extract, or may contain other ingredients.

The content of the sugar-free pineapple extract in the hyaluronidase activity inhibitor is not particularly limited and may be appropriately selected depending on the purpose.

The transglutaminase-1 production promoting agent of the present invention may be composed of only the sugar-free pineapple extract, or may contain other components.

The content of the sugar-free pineapple extract in the transglutaminase-1 production promoter is not particularly limited and may be appropriately selected depending on the purpose.

The melanin production inhibitor of the present invention may be composed of only the sugar-free pineapple extract, or may contain other components.

The content of the sugar-free pineapple extract in the melanin production inhibitor is not particularly limited and may be appropriately selected depending on the purpose.

The dermal papilla cell growth promoter of the present invention may be composed of only the sugar-free pineapple extract or may contain other components.

The content of the sugar-free pineapple extract in the dermal papilla cell growth promoter is not particularly limited and may be appropriately selected according to the purpose.

(Other components)

The sugar-free pineapple extract may be formulated into a desired formulation such as powder, granule, tablet, liquid or the like according to a conventional method using a pharmaceutically acceptable carrier such as dextrin, cyclodextrin or the like or any other auxiliary agent And may be used in combination with other compositions (for example, oral medicines, etc.), as well as ointments, external liquid preparations, patches and the like. At this time, examples of the adjuvant include excipients, binders, disintegrators, lubricants, stabilizers, mating agents, and fodders.

Examples of the excipient include lactose, white sugar, sodium chloride, glucose, starch, calcium carbonate, kaolin, microcrystalline cellulose and silicic acid. Examples of the binder include water, ethanol, propanol, single syrup, glucose, starch solution, gelatin solution, carboxymethylcellulose, hydroxypropylcellulose, hydroxypropyl starch, methylcellulose, ethylcellulose, shellac, Calcium phosphate, and polyvinyl pyrrolidone. Examples of the disintegrators include dry starch, sodium alginate, agar, sodium hydrogen carbonate, calcium carbonate, sodium lauryl sulfate, stearic acid monoglyceride, and lactose. Examples of the lubricant include purified talc, stearate, borax, polyethylene glycol, and the like. Examples of the stabilizer include sodium pyrophosphate, EDTA, thioglycolic acid, and thioerythritol. Examples of the mating agents and the fibrillating agent include white sugar, orange peel, citric acid, tartaric acid and the like.

The laminin-5 production promoter, epidermal hyaluronic acid production promoter, hyaluronidase activity inhibitor, transglutaminase-1 production promoter, melanin production inhibitor and dermal papilla cell growth promoter of the present invention may be added to the laminin- Other natural extracts having any one of production-promoting action, epidermal hyaluronic acid production promoting action, hyaluronidase activity inhibiting action, transglutaminase-1 production promoting action, melanin production inhibiting action and dermal papilla cell growth promoting action And can be used in combination.

The laminin-5 production promoter exhibits laminin-5 production promoting action by the action of the sugar-free pineapple extract contained as an active ingredient.

The epidermal hyaluronic acid production promoter exerts the epidermal hyaluronic acid production promoting action by the action of the sugar-free pineapple extract contained as an active ingredient.

The hyaluronidase activity inhibitor exerts the hyaluronidase inhibitory action by the action of the sugar-free pineapple extract contained as an active ingredient.

The transglutaminase-1 production promoter exhibits a transglutaminase-1 production promoting action by the action of the sugar-free pineapple extract contained as an active ingredient.

The melanin production inhibitor exhibits an action of inhibiting melanin production by the action of the sugar-free pineapple extract contained as an active ingredient.

The dermal papilla cell proliferation promoter exerts the dermal papilla cell proliferation promoting action by the action of the sugar-free pineapple extract contained as an active ingredient.

According to the laminin-5 production promoter of the present invention, it is possible to prevent or ameliorate the aging symptoms of the skin such as wrinkles and elasticity degradation by promoting the formation of basement membrane of the skin, for example, through an excellent laminin-5 production promoting action. However, the laminin-5 production promoter of the present invention can be used for all purposes which have a significance to exert the laminin-5 production promoting action in addition to these uses.

According to the epidermal hyaluronic acid production promoter of the present invention, it is possible to prevent and improve aging symptoms of the skin such as skin roughness, wrinkles and elasticity by improving the moisture and elasticity through the action of promoting the production of excellent epidermal hyaluronic acid . However, the epidermal hyaluronic acid production promoter of the present invention can be used for all purposes that have an effect of promoting the production of epidermal hyaluronic acid in addition to these uses.

According to the hyaluronidase activity inhibitor of the present invention, excellent hyaluronidase inhibitory activity is inhibited, for example, by inhibiting the degradation of hyaluronic acid, promoting histamine release, enhancing moisturization, Can be prevented and improved. However, the hyaluronidase activity inhibitor of the present invention can be used for all purposes that have an effect of inhibiting hyaluronidase activity in addition to these uses.

According to the transglutaminase-1 production promoter of the present invention, the excellent transglutaminase-1 production promoting action improves, for example, the barrier function of the skin epidermis, thereby improving skin roughness, loss of skin texture, It is possible to prevent or ameliorate the aging symptoms of the skin, such as lowering of the age. However, the transglutaminase-1 production promoter of the present invention can be used for all purposes that have a significance to exhibit transglutaminase-1 production promoting action in addition to these uses.

According to the melanin production inhibitor of the present invention, it is possible to prevent and improve the symptoms of aging of the skin such as darkening of the skin, stain and the like by protecting the skin from ultraviolet rays, for example, through an excellent melanin production inhibiting action. However, the melanin production inhibitor of the present invention can be used for all purposes which have a significance to exhibit melanin production inhibitory action in addition to these uses.

According to the dermal papilla cell growth promoter of the present invention, it is possible to prevent and improve fine hairs by promoting proliferation of hair cell epithelial cells and differentiation of hair through excellent dermal papilla cell proliferation promoting action. However, the agent for promoting proliferation of the dermal papilla cell can be used for all purposes which have a significance to exert the action of promoting the growth of the papilla cell, in addition to these uses.

The laminin-5 production promoter, epidermal hyaluronic acid production promoter, hyaluronidase activity inhibitor, transglutaminase-1 production promoter, melanin production inhibitor and papillary cell proliferation promoter of the present invention are excellent in action As an extract obtained from pineapple, it is excellent in safety and is therefore very suitable for compounding in food and drink, external preparation for skin and hair restorer.

In addition, since the laminin-5 production promoter, epidermal hyaluronic acid production promoter, hyaluronidase activity inhibitor, transglutaminase-1 production promoter, melanin production inhibitor and dermal papilla cell growth promoter of the present invention have excellent action, -5, epidermal hyaluronic acid, hyaluronidase, transglutaminase-1 and dermal papilla cells, hyaluronidase, laminin-5, epidermal hyaluronic acid, transglutaminase-1, melanin and breast milk It can be suitably used as a reagent for studying functions or diseases related to cells.

In addition, the laminin-5 production promoter, epidermal hyaluronic acid production promoter, hyaluronidase activity inhibitor, transglutaminase-1 production promoter, melanin production inhibitor and dermal papilla cell growth promoter of the present invention are suitably applied to human beings However, it may be applied to animals other than humans as long as each action and effect is exerted.

(Food and drink)

The food or drink of the present invention is characterized by containing the sugar-free pineapple extract of the present invention, the laminin-5 production promoter, epidermal hyaluronic acid production promoter, hyaluronidase activity inhibitor, transglutaminase-1 production promoter, melanin production inhibitor, And a cell proliferation promoting agent, and further contains other components as necessary.

The food or beverage may be a food or beverage containing the sugar-free pineapple extract as an active ingredient, or a food or beverage containing the sugar-free pineapple extract as a main ingredient, so as not to interfere with its activity. The food product may be the sugar-free pineapple extract itself.

The term "food or drink" refers to a substance that is ingested by the oral or gastrointestinal tract administration in ordinary social life, and is limited to the classification of foods, medicines, quasi-drugs, etc. For example, it means to include a wide range of general foods, health foods, health functional foods, cosmetics, quasi-drugs and medicines that are ingested orally.

Further, the food or drink of the present invention can be easily ingested by processing into a shape such as a beverage, a hard capsule, a soft capsule or a granule, so that it can be widely used.

The food or drink can be appropriately selected according to the purpose without any particular limitation, and examples thereof include beverages such as soft drinks, carbonated beverages, nutritional drinks, fruit drinks, lactic drinks, etc. (including concentrates and adjusting powders of these drinks); Frozen confectionery such as ice cream, ice sherbet, and bean curd; Noodles such as soba, udon, mung bean noodle, dumpling, shamapi, Chinese noodle, instant noodle; Confectionery such as candy, candy, gum, chocolate, jelly, snack, biscuit, jelly, 쨈, cream, baked confection, bread; Aquatic products such as crab, salmon, clam, tuna, sardine, shrimp, bonito, mackerel, whale, oyster, saury, squid, egg shell, scallop, abalone, sea urchin, salmon roe, Fish / livestock processed foods such as fish paste, ham, sausage; Dairy products such as processed milk and fermented milk; Preserved and preserved processed foods such as salad oil, tempura oil, margarine, mayonnaise, shortening, whipping cream, dressing; Seasonings such as sauces, sauces and the like; Curry, situ, chicken egg bowl, porridge, nutritional porridge, Chinese bowl, pork cutlet, fried rice bowl, eel rice bowl, hash rice, oden, maple tofu, roasted meatballs, meat sauce, egg soup, omelet rice, dumplings, , Retort pouch foods such as meatballs; Side dishes such as salads and pickles; Various forms of health / beauty / nutritional supplements; Tablets, granules, capsules, drinks, medicines such as troches, and quasi drugs. Further, the food or drink is not limited to the above example.

The food or drink according to any one of claims 1 to 3, wherein the food or drink contains at least one of sugar-free pineapple extract, laminin-5 production promoter, epidermal hyaluronic acid production promoter, hyaluronidase activity inhibitor, transglutaminase- 1 production promoter, melanin production inhibitor, Can not be uniformly defined depending on the food or drink to be added. In the case of tablets, capsules, etc., the content is preferably 1% by mass to 90% by mass in terms of the amount of the sugar-free pineapple extract , And in other foods and drinks, it is preferably 0.001% by mass to 50% by mass in terms of the amount of the sugar-free pineapple extract. It is preferable that the intake amount of the sugar-free pineapple extract per adult is about 1 mg to 1,000 mg per day considering the general intake amount of the food to be added.

Examples of the other components include auxiliary raw materials and additives commonly used for producing the food and drink.

The auxiliary raw materials and additives may be appropriately selected according to the purpose without any particular limitation, and examples thereof include glucose, fructose, sucrose, maltose, sorbitol, stevioside, rubusoide, corn syrup, lactose, citric acid, But are not limited to, malic acid, succinic acid, lactic acid, L-ascorbic acid, d1-? -Tocopherol, sodium erishorbate, glycerin, propylene glycol, glycerin fatty acid esters, polyglycerin fatty acid esters, sucrose fatty acid esters, sorbitan fatty acid esters, Casein, gelatin, pectin, agar, vitamin B, nicotinic acid amide, calcium pantothenate, amino acids, calcium salts, coloring matters, fragrances and preservatives.

The food or drink according to the present invention can be orally ingested on a routine basis. By the action of the pineapple extract as an active ingredient, the hyaluronidase activity inhibiting action, the laminin-5 production promoting action, the epidermal hyaluronic acid production promoting action, It is possible to achieve at least one of the further production promotion action and the dermal papilla cell proliferation promotion action very effectively.

In addition, the food or drink of the present invention is suitably applied to humans, but may be applied to animals other than humans as long as the respective action and effect are exerted.

(External preparation for skin)

The external preparation for skin of the present invention is characterized by containing the sugar-free pineapple extract of the present invention, the laminin-5 production promoter, epidermal hyaluronic acid production promoter, hyaluronidase activity inhibitor, transglutaminase-1 production promoter, melanin production inhibitor, And a cell proliferation promoting agent, and further contains other components as necessary.

Herein, the external preparation for skin refers to various medicines to be applied to the skin. The classification is not particularly limited, and includes, for example, skin cosmetics, quasi-drugs, medicines and the like in a wide range.

The external preparation for skin may be an external preparation for skin which contains the sugar-free pineapple extract as an active ingredient, or a skin external preparation containing the sugar-free pineapple extract as a main component so as not to interfere with its activity. In addition, the external preparation for skin may be the sugar-free pineapple extract itself.

The external preparation for skin may be appropriately selected according to the purpose without any particular limitation and examples thereof include ointments, creams, emulsions, essences, lotions, packs, foundation, lip balm, bath salts, hair tonic, hair lotion, soap, body shampoo, have.

The above-mentioned other components can be appropriately selected according to the purpose without particular limitation, and can be appropriately selected in accordance with the purpose and can be appropriately selected in accordance with the components conventionally used in the preparation of the external preparation for skin such as astringent, antiseptic, antibacterial agent, whitening agent, ultraviolet absorber, An antioxidant, an active oxygen scavenger, a fat, a lead, a hydrocarbon, a fatty acid, an alcohol, an ester, a surfactant, and a fragrance.

The external preparation for skin according to any one of claims 1 to 3, wherein the dermal pineapple extract, laminin-5 production accelerator, epidermal hyaluronic acid production accelerator, hyaluronidase activity inhibitor, transglutaminase-1 production accelerator, melanin production inhibitor, May be appropriately selected depending on the kind of the external preparation for skin and the like without particular limitation. For example, it is preferably 0.0001% by mass to 10% by mass in terms of the amount of the sugar-free pineapple extract, more preferably 0.001% by mass to 5% % By mass is more preferable.

(Hair restoration)

The hair restorer of the present invention is a hair restorer comprising the saccharide-free pineapple extract of the present invention, a laminin-5 production promoter, a skin hyaluronic acid production promoter, a hyaluronidase activity inhibitor, a transglutaminase-1 production promoter, a melanin production inhibitor, And a growth promoting agent, and further contains other components as necessary.

The hair restoring agent may be one which is blended with an optional hair restorer so as not to interfere with the activity of the sugar-free pineapple extract, or may be a hair growth agent containing the sugar-free pineapple extract as a main component. In addition, the hair restorer may be the sugar-free pineapple extract itself.

According to the hair restoration of the present invention, excellent hair laminin-5 production promoting action, epidermal hyaluronic acid production promoting action, hyaluronidase activity inhibiting action, transglutaminase-1 production promoting action, melanin production inhibiting action, For example, by promoting the proliferation of papillary cells and embryonic epithelial cells, differentiation of hair, and the like, and can be suitably used for preventing and improving hair loss, hair loss and the like.

The term "hair restorer" as used herein refers to a hair restorer that can be used to develop a hair, to promote hair growth, to maintain a mock health condition, to prevent hair loss, and to reduce hair loss Quot; and " hair dye ". Therefore, the hair restorer of the present invention can be suitably used as a hair restorer.

The hair restorer of the present invention has an excellent action, and is excellent in feeling and safety when applied to the skin, and therefore, is well suited for compounding in external preparations for skin such as hair restorers and hair cosmetics. The administration site of the external preparation for skin is preferably, for example, hair such as hair, scalp hair, scalp, sebaceous gland of scalp, and the like. Examples of the formulations of the external preparation for skin include ointments, creams, lotions, gels, sols, sprays, solutions and the like. Hair cosmetics include, for example, hair tonic, hair liquid, shampoo, pomade, and rinse.

In the hair restorer, it is preferred that the sugar-free pineapple extract, laminin-5 production promoter, epidermal hyaluronic acid production promoter, hyaluronidase activity inhibitor, transglutaminase-1 production promoter, melanin production inhibitor, The content of any one can be appropriately selected according to the kind of the external preparation for skin and the like without particular limitation. For example, it is preferably 0.0001% by mass to 10% by mass in terms of the amount of the sugar-free pineapple extract, more preferably 0.001% % Is more preferable.

(Example)

Hereinafter, the present invention will be described more specifically by way of Production Examples and Test Examples, but the present invention is not limited thereto.

(Preparation 1: Preparation of sugar-free pineapple extract)

100 g of the residue (pineapple pulp) after the compression of the pineapple edible portion was added to 1,000 ml of 90% by volume ethanol, and the mixture was heated and extracted with a reflux extractor at 80 DEG C for 2 hours, followed by thermal filtration. Thereafter, the mixture was concentrated under reduced pressure using an evaporator, and the same filtration treatment was carried out again. The obtained residue was washed with 500 ml of water to obtain 1.5 g of paste-free pineapple extract in the form of a saccharified saccharide-free paste. The yield of the extract was 1.5 (mass%).

The resulting sugar-free pineapple extract was subjected to component analysis as follows.

The glucose / fructose assay kit (F-kit (glucose / fructose), manufactured by JK International, Inc., Japan) was prepared by dissolving the sugar-free pineapple extract in 50 vol% ethanol at a sample concentration of 20 vol% ) To measure the content of glucose and fructose. As a result of the measurement, it was found that the sugar-free pineapple extract contained 0.03 mass% of glucose and 0.01 mass% of fructose.

Using the sugar-free pineapple extract obtained in Preparation Example 1 as a test sample, evaluation of skin improvement, whitening, and hair growth of the sugar-free pineapple extract was carried out as shown in Test Example 1-6 below .

Further, as the control pineapple extract, 26.3 g of the frozen pineapple frozen product obtained by concentrating 200 ml of 100% pineapple juice (commercially available pineapple juice) under reduced pressure using an evaporator and using freeze-drying was used. The content of glucose and fructose was measured in the same manner as in the sugar-free pineapple extract to 0.413 g / L of the pineapple freeze-dried product. The pineapple frozen product contained 29.1 mass% glucose and 26.6 mass% fructose Could know.

(Test Example 1: Laminin-5 production promoting action test)

<Test Method>

The human normal neonatal skin epidermal keratinocytes (NHEK, manufactured by Kuraray Co., Ltd.) were cultured using a medium for human normal normal neonatal epidermal keratinocytes (KGM, Kurashiki Denshi Co., Ltd.), and cells were recovered by trypsin treatment. The recovered cells were diluted with KGM-BPE (KGM-BPE, manufactured by Kurashiki Denshi Co., Ltd.) so as to have a concentration of 1 × 10 ⁵ cells / mL, and then seeded at 500 μl per well in a 24-well plate and cultured for 2 days. After completion of the culture, the medium was removed and 500 μl of a test sample dissolved in KGM-BPE was added to the wells and cultured for 48 hours. After completion of the incubation, 100 상 of the supernatant was transferred to an ELISA plate and adsorbed on a plate at 37 캜 for 2 hours. The solution was discarded and washed with a 0.05% (v / v) PBS Respectively.

Thereafter, the blocking operation was performed with a living-sanri buffer (pH 7.4) containing 1 vol% bovine serum albumin. The solution was discarded and washed with 0.05% PBS-T to react with an anti-human laminin-5 antibody (mouse IgG, manufactured by Nippon Chemicon Co., Ltd.). The solution was discarded and washed with 0.05% PBS-T, followed by color development. From the obtained measurement results, the laminin-5 production promotion rate (%) was calculated by the following formula.

The calculation method of laminin-5 production promoting action is as follows. The results are shown in Table 1.

[Equation 1]

Laminin-5 production promotion rate (%) = A / B x 100

(Wherein, in the above formula 1, A: absorbance at a wavelength of 405 nm when a test sample is added

B: absorbance at a wavelength of 405 nm (control) at the time of no sample addition)

Concentration (/ / ml) Laminin-5 production promotion rate (%) Sugar-free pineapple extract Pineapple juice lyophilisate 2.5 114.3 ± 3.3 ^** 91.3 ± 5.8 10 121.3 ± 3.0 ^** 88.7 ± 2.2

Mean ± SE, n = 4, ^** : p <0.01

From the results shown in Table 1, concentration-dependent stimulation of laminin-5 production was recognized in the sugar-free pineapple extract.

(Test Example 2: Test for promoting epidermal hyaluronic acid production)

<Test Method>

Human normal neonatal skin epidermal keratinocytes (NHEK) were cultured using human normal neonatal epidermal keratinocyte medium (KGM), and then cells were recovered by trypsin treatment. The recovered cells were diluted with KGM to a concentration of 1 × 10 ⁵ cells / mL, and then seeded in a 96-well plate at a rate of 100 μl per well, followed by culturing for 24 hours. After completion of the cultivation, 100 μl of a test sample dissolved in KGM was added to each well and cultured for 7 days. After culturing, the amount of hyaluronic acid in the medium of each well was measured by a sandwich method using hyaluronic acid binding protein (HABP, manufactured by Biochemical Bio-business Co., Ltd.).

The calculation method of hyaluronic acid production promoting action is as follows. The results are shown in Table 2.

&Quot; (2) &quot;

Acceleration rate of hyaluronic acid production per well (%) = A / B x 100

(Wherein, in the formula 2,

A: Amount of hyaluronic acid at the time of addition of the test sample

B: the amount of hyaluronic acid at the time of no sample addition)

Concentration (/ / ml) Promotion rate of epidermal hyaluronic acid production (%) Sugar-free pineapple extract Pineapple juice lyophilisate 25 108.5 ± 1.3 ^** 78.8 ± 4.4 100 149.7 ± 2.1 ^*** 79.6 ± 4.6

Mean ± SE, n = 5, ^** : p <0.01, ^*** : p <0.001

From the results shown in Table 2, significant action of promoting epidermal hyaluronic acid production was recognized in the sugar-free pineapple extract.

(Test Example 3: Hyaluronidase Inhibitory Activity Test)

<Test Method>

0.1 mL of a hyaluronidase solution (Type IV-S (origin of origin), 400 NF units / mL; manufactured by Sigma-Aldrich Japan) was added to 0.2 mL of a 0.1 mol / L acetic acid buffer solution (pH 3.5) Lt; 0 &gt; C for 20 minutes. Further, 0.2 mmol of 2.5 mmol / L calcium chloride as an activator was added and the mixture was reacted at 37 캜 for 20 minutes. To this was added 0.5 mL of a 0.4 mg / ml sodium hyaluronate solution (sodium hyaluronate from chicken cow, manufactured by Wako Pure Chemical Industries, Ltd.), and the mixture was reacted at 37 캜 for 40 minutes. Thereafter, 0.2 mL of 0.4 mol / L sodium hydroxide was added to stop the reaction. After cooling, 0.2 mL of a boric acid solution was added to each reaction solution, followed by boiling for 3 minutes. After cooling, 6 mL of p-DABA reagent (p-dimethylaminobenzaldehyde, manufactured by Wako Pure Chemical Industries, Ltd.) was added and the mixture was reacted at 37 ° C for 20 minutes. Thereafter, the absorbance at a wavelength of 585 nm was measured. Blank tests were carried out in the same way and corrected.

The calculation method of hyaluronidase activity inhibitory action is as follows. The results are shown in Table 3.

&Quot; (3) &quot;

Hyaluronidase activity inhibition rate (%) = {1- (St-Sb) / (Ct-Cb)} 100

(Wherein, in the above formula (3)

St: absorbance at wavelength of 585 nm of the test sample solution

Sb: wavelength of test sample solution blank absorbance at 585 nm

Ct: absorbance of the control solution at a wavelength of 585 nm

Cb: absorbance of the control solution blank at a wavelength of 585 nm)

Concentration (/ / ml) Hyaluronidase activity inhibition rate (%) Sugar-free pineapple extract Pineapple juice lyophilisate 2.5 27.6 -22.6

From the results in Table 3, hyaluronidase inhibitory action was recognized in the sugar-free pineapple extract. In addition, it was confirmed that the frozen pineapple frozen product had an action to promote hyaluronidase activity.

(Test Example 4: Transglutaminase-1 Production Promotion Test)

<Test Method>

Human normal neonatal skin epidermal keratinocytes (NHEK) were cultured using human normal neonatal epidermal keratinocyte medium (KGM), and then cells were recovered by trypsin treatment. The recovered cells were diluted with KGM to a concentration of 1 × 10 ⁵ cells / mL, and then seeded at 100 μl per well in a 96-well plate and cultured for 2 days. After the completion of cultivation, 100 μl of a test sample dissolved in KGM was added to each well and cultured for 24 hours. After the incubation, the medium was removed, and the cells were fixed on a plate. The amount of transglutaminase-1 expressed on the cell surface was measured with a monoclonal anti-keratinocyte transglutaminase-1 antibody (Biomedical Technologies. Inc.).

The calculation method of the transglutaminase-1 production promoting action is as follows. The results are shown in Table 4.

&Quot; (4) &quot;

Transglutaminase-1 production promotion rate (%) = A / B x 100

(Wherein, in the formula 4,

A: absorbance at a wavelength of 405 nm upon addition of a test sample

B: absorbance at a wavelength of 405 nm (control) at the time of no sample addition)

Concentration (/ / ml) Transglutaminase -1 production promotion rate (%) Sugar-free pineapple extract Pineapple juice lyophilisate 100 122.2 ± 2.8 ^*** 99.5 ± 1.0

Mean ± SE, n = 6, ^*** : p <0.001

From the results in Table 4, significant transglutaminase-1 production promoting action was recognized in the sugar-free pineapple extract.

(Test Example 5: Test for inhibiting melanin production)

<Test Method>

B16 melanoma cells were cultured using Dulbecco's MEM (Dulbecco's modified Eagle's medium (1), Nissui Pharmaceutical Co., Ltd.) containing 10 vol% FBS (STANDARD FETAL BOVINE SERUM, made by HyClone) Respectively. The recovered cells were diluted with Dulbecco's MEM containing 10% FBS and 1 mmol / L theophylline (manufactured by Wako Pure Chemical Industries, Ltd.) to a concentration of 24.0 × 10 ⁴ cells / mL, And cultured for 6 hours. After completion of the culture, 300 μL of a test sample dissolved in Dulbecco's MEM containing 10% by volume of FBS and 1 mmol / L of theophylline was added to each well and cultured for 4 days. After completion of the culturing, the medium was removed from each well, 200 쨉 l of a 2 mol / L NaOH solution was added thereto, the cells were destroyed by an ultrasonic disrupter, and the absorbance at a wavelength of 475 nm was measured to determine the amount of melanin production.

In order to measure the cell viability, the cells were incubated in the same manner and then washed with 400 μl of PBS (-) phosphate physiological buffer to obtain 13.8 mmol / L neutral solution in Dulbecco's MEM containing 10% by volume FBS at a final concentration of 0.05 mg / 200 쨉 l of red was added to each well. After 2.5 hours of incubation, the neutral red solution was discarded, and 200 μl of an ethanol / acetic acid solution (ethanol: acetic acid: water = 50: 1: 49) was added to each well to extract the pigment. After the extraction, the absorbance at a wavelength of 540 nm was measured.

Cells cultured with Dulbecco's MEM containing 10 vol% FBS and 1 mmol / L theophylline were also tested in the same manner.

The calculation method of the melanin production inhibitory action is as follows. The results are shown in Table 5.

&Quot; (5) &quot;

Melanin production inhibition rate (%) = {1- (B / D) / (A / C)} 100

(In the formula 5,

A: Absorbance at a wavelength of 475 nm in cells to which no test sample was added

B: Absorbance at a wavelength of 475 nm in the cells to which the test sample was added

C: Absorbance at a wavelength of 540 nm in a cell to which no test sample is added

And D: the absorbance at 540 nm in the wavelength of the cell to which the test sample is added.

Concentration (/ / ml) Melanin production inhibition (%) Sugar-free pineapple extract Pineapple juice lyophilisate 12.5 14.2 ± 1.4 6.5 ± 0.5 25 13.8 ± 1.3 8.8 ± 4.6 50 20.4 ± 1.6 11.6 ± 1.8 100 23.0 ± 1.6 15.4 ± 2.8

From the results in Table 5, it was recognized that the sugar-free pineapple extract had a stronger and more concentration-dependent inhibitory action on melanin production than the pineapple frozen lyophilized product of the control group.

(Test Example 6: Test for promoting the proliferation of the dermal papilla cell)

<Test Method>

Normal human hair dermal papilla cells (manufactured by Dong Yang Dairy Co., Ltd.) were cultured using a dermal papilla cell growth medium (manufactured by Dong Yang Dairy Co., Ltd.), and cells were recovered by trypsin treatment. The recovered cells were diluted to a concentration of 1.0 x 10 ⁴ cells / mL using Dulbecco's MEM (Dulbecco's modified Eagle medium (1), manufactured by Nissui Pharmaceutical Co., Ltd.) containing 10 vol% FBS, 200 [mu] l per well of the plate was inoculated and cultured for 3 days. After the culture, the medium was removed, and 200 μl of a test sample dissolved in serum-free DMEM (Dulbecco's modified Eagle medium (1), manufactured by Nissui Pharmaceutical Co., Ltd.) was added to each well and further cultured for 4 days. The proliferation of dermal papilla cells was measured using an MTT assay. After the completion of the incubation, the medium was removed and 3- (4,5-dimethylthiazol-2-yl) -2,5-diphenyltetrazolium bromide (MTT, manufactured by Mitsubishi Chemical Corporation) dissolved in serum-free DMEM to a final concentration of 0.4 mg / Ltd.) was added to each well. After incubation for 2 hours, the blue formazan produced in the cells was extracted with 100 2- of 2-propanol. After the extraction, the absorbance (OD ₅₇₀ ) at a wavelength of 570 nm was measured. At the same time, the absorbance (OD ₆₅₀ ) at a wavelength of 650 nm was measured as turbidity, and the difference between them was defined as the amount of blue formazan produced.

The calculation method of promoting the proliferation of the dermal papilla cell is as follows. The results are shown in Table 6.

&Quot; (6) &quot;

Promoting rate of proliferation of dermal papilla cells (%) = A / B x 100

(Wherein, in the formula 6,

A: OD _570- OD ₆₅₀ at the time of addition of the test sample

B: OD _{570 -} OD ₆₅₀ at the time of no addition of the test sample).

Concentration (/ / ml) Promoting rate of proliferation of dermal papilla cells (%) Sugar-free pineapple extract Pineapple juice lyophilisate 25 114.3 ± 1.1 ^*** 99.0 ± 1.8

Mean ± SE, n = 6, ^*** : p <0.001

From the results in Table 6, it was confirmed that the promoting action of the papilla cell proliferation was significant only in the sugar-free pineapple extract.

Industrial availability

The present invention relates to a method for producing a saccharide-free pineapple extract, a laminin-5 production accelerator, a epidermal hyaluronic acid production accelerator, a hyaluronidase activity inhibitor, a transglutaminase-1 production accelerator, a melanin production inhibitor, 5, which is capable of being consumed on a daily basis, and at the same time being inexpensive, has excellent laminin-5 production promoting action, epidermal hyaluronic acid production promoting action, hyaluronidase activity inhibiting action, transglutaminase-1 production promoting action, melanin production inhibiting action And at least one of the action of promoting the proliferation of papilla cells, it can be suitably used as a reagent for research, or it can be blended with a cosmetic food or drink, blended with an external preparation for skin and hair restorer, or used as a research reagent.

Claims (1)

All inventions described herein