TWI500428B - Sugar-free pineapple extract, method for producing the extract, and application of the extract - Google Patents

Description

Pineapple extract without sugar, method for producing the same, and use thereof

The present invention relates to a pineapple extract containing no sugar, a method for producing the same, a laminin- 5 production promoter, a hyaluronic acid production promoter, and a hyaluronic agent. Acidase (hyaluronidase) activity inhibitor, transglutaminase-1 production promoter, melanin production inhibitor, dermal papilla cell proliferation promoter, food and drink, skin external preparation, and hair growth Agent.

The skin is composed of the epidermis, basement membrane, and dermis. The dermis is composed of fibroblasts, collagen fibers secreted by the cells, elastin (elastane fibers), and extracellular matrices such as hyaluronic acid. The basement membrane is deposited at the junction between the epidermis and the dermis, and not only maintains the epidermis and the dermis, but also plays an important role in maintaining the skin function (see Non-Patent Document 1). In young skin, by maintaining the consistency of the interaction of these skin tissues, water retention, softness, elasticity, and the like are ensured, and the appearance is also firm and moist, and maintains a delicate state.

However, due to the external factors such as ultraviolet rays, significant air drying, excessive skin washing, stress, smoking, etc., as the aging progresses, the decomposition or deterioration of the collagen fibers which are the main constituents of the extracellular matrix will occur. When the proliferation of fibroblasts is slow, or laminin-5 which is a major component of the basement membrane is decomposed or deteriorated, the basement membrane structure is destroyed (see Non-Patent Document 2). Further, it causes a decrease in the amount of hyaluronic acid produced as a natural moisturizing factor. This result may be an aging symptom such as a moisturizing function or a decrease in elasticity of the skin, abnormal peeling of keratin, wrinkles, dullness, loss of texture, and reduction in elasticity.

Therefore, it is generally believed that by promoting the production of laminin-5 and the production of hyaluronic acid, it is possible to prevent or even improve the aging of the skin. Therefore, it is expected that a substance having a laminin-5 production promoting action and a hyaluronic acid production promoting action can be obtained from a natural product having high safety.

Heretofore, in the case of having a laminin-5 production promoting effect, for example, Puerariae radix extract, licorice extract or flavonoid fraction thereof, and fan (Iris domestica) have been reported. Extract, Alstonia scholaris extract, Tinospora cordifolia extract, fenugreek extract, whey extract (above, refer to Patent Document 1), red stalk (Ononis spinosa) extract, Medicago falcata extract, bean sprout extract, Vigna angularis extract (refer to Patent Document 2 above), soybean extract (see Patent Document 3), and the like. In addition, it is attempting to extract a substance having a hyaluronic acid production promoting action from a natural product (see Non-Patent Document 3).

On the other hand, inflammatory diseases, for example, contact dermatitis (rash), dry phlegm, pemphigus vulgaris, other skin diseases associated with rough skin, and pathogenesis are Everything is different. For this reason, it is known to be derived from hyaluronidase activity.

The hyaluronate which retains affinity in a living tissue is decomposed by ultraviolet rays, enzymes, etc. in an aqueous system, and the water retention effect is also reduced with a decrease in molecular weight. Moreover, hyaluronic acid is present in intercellular tissues and is also involved in vascular permeability. Furthermore, it is generally believed that hyaluronidase is activated by mast cells in relation to degranulation from mast cells. Therefore, by inhibiting the hyaluronidase activity of hyaluronic acid hydrolyzing enzyme, the hyaluronic acid can be stabilized, and the release of chemical mediators such as histamine from mast cells can be prevented, and moisturizing and anti-inflammatory can be expected.

For example, an extract of a plant of the genus Osbeckia (see Patent Document 4) and an extract of vine tea (see Patent Document 5) have been reported as a herbal medicine having such a hyaluronidase activity. Rosemary extract, thyme extract, and Melissa extract (see Patent Document 6).

Further, the epidermis frequently produces new keratinocytes via division and subsequent differentiation of keratinocytes, and has a defense function of preventing skin from being irritated by various external stimuli. In particular, in the differentiation process of keratinocytes, proteins from the spine layer to the granular layer, involucrin, etc., are expressed by the action of the enzyme branamine transferase-1 to form an embedding angle. The cornified envelope (CE) of the insoluble cell membrane-like structure of the cells, and the cell bone and structural stability of the keratinocytes are imparted.

However, due to various factors, the amount of production of the branamine transferase-1 in the epidermis is reduced, and the formation of the keratinized envelope (CE) is incomplete, and the keratinization does not proceed normally. As a result, it is generally considered to be a skin condition such as a decrease in keratin barrier function and a decrease in skin moisturizing function, rough skin or dry skin.

Because of this, it is considered that by increasing the production of glutamine transductase-1 in the epidermis of keratinocytes, the formation of keratinized envelope is promoted to normalize keratinization, and external stimuli accompanying drying or ultraviolet rays can be suppressed. The lowering of the skin barrier function can prevent and improve various skin symptoms such as dry skin or rough skin.

The melanin on the skin also has the task of protecting the body from UV damage, but over-production or uneven accumulation is the cause of blackening or even spots on the skin. In general, it is changed from tyrosine to dopa (dihydroxyphenylalanine) and from dopa to dopaquinone by the action of the enzyme tyrosinase synthesized in the pigment cells. Melanin is formed via an intermediate such as 5,6-dihydroxyindophenol (indophenol). Therefore, in order to prevent and improve the color darkening of the skin (skin pigmentation), that is, to suppress the production of melanin for whitening, or to bleach the already produced melanin, it is generally considered to be effective. Because of this background, the development of substances that inhibit the production of melanin and cause whitening effects has become so prevalent.

The growth of the hair is repeated by the hair cycle of the cycle of the growth phase, the regression period, and the rest period. In this hair cycle, the stage of formation of a new hair bag from the rest period to the growth stage is generally considered to be the most important one in the growth hair. However, those responsible for the proliferation and differentiation of hairpin epithelial cells in this stage are generally considered to be dermal papilla cells. The dermal papilla cell line is located in the inner side of the hairy epithelial cells composed of outer root sheath cells and stromal cells near the hairy root, and is located in the root part of the root of the hairy root embedded in the basement membrane, which promotes the hair follicle epithelial cells and promotes it. Proliferation and the like are responsible for important tasks in hair differentiation (see Non-Patent Document 4).

On the other hand, it has been reported that the pineapple fruit juice extract or the pineapple fruit extract is used as a skin external preparation for whitening and skin component (see Patent Document 7).

However, it is excellent in safety and productivity, and can be taken daily and is inexpensive, and has excellent laminin-5 production promoting action, epidermal hyaluronic acid production promoting action, hyaluronidase activity inhibiting effect, and branamide The need for the various preparations of the natural system of at least one of the transferase-1 production-promoting action, the melanin production-inhibiting action, and the dermal papilla cell proliferation-promoting action is extremely strong, and the satisfactory system is not provided as the present state. .

[Previous Technical Literature]

Patent literature

PTL1 Special Open 2003-137767

PTL 2 Special Publication No. 2003-137768

PTL3 Laid-open No. 2004-217618 Gazette

PTL4 JP 2003-055242 Gazette

PTL5 Special Opening 2003-012532

PTL6 Special Opening No. 8-333267

PTL7 special opening 2002-275081

Non-patent literature

NPL1 Marinkovich MP et al., J. Cell. Biol. 1992 199:695-703

NPL2 Lavker et al., J. Invest. Dermatol. 1979 73:59-66

NPL 3 "Fragrance Journal" 1992, No. 11, p43

NPL4 "Trends Genet", 1992, No. 8, p.56-61

The present invention has been made to solve the above-mentioned problems and achieve the following objects. That is, the present invention provides daily safety and productivity, is excellent in daily intake, and is inexpensive, and has excellent laminin-5 production promoting action, epidermal hyaluronic acid production promoting action, and hyaluronidase activity inhibiting action. An effect of a non-sugar pineapple extract containing at least one of a branamine transferase-1 production promoting action, a melanin production inhibiting action, and a dermal papilla cell proliferation promoting action, and a method for producing the same.

Further, the present invention has an object of providing a laminin-5 production promoter having excellent laminin-5 production-promoting action and high safety. Further, the present invention has an object of providing a skin hyaluronic acid production promoter having an excellent epidermal hyaluronic acid production promoting action and high safety. Further, the present invention has an object of providing a hyaluronidase activity inhibitor having excellent hyaluronidase activity inhibitory action and high safety. Further, the present invention has an object of providing a branamine transferase-1 production promoter having excellent branamine transferase-1 production-promoting action and high safety. Further, the present invention has an object of providing a melanin production inhibitor having excellent melanin production inhibitory action and high safety. Further, the present invention has an object of providing a dermal papilla cell proliferation promoter having excellent dermal papilla cell proliferation action and high safety. Moreover, the present invention has an object of providing a food or drink, an external preparation for skin, and a hair growth agent which are characterized by containing the above-mentioned pineapple extract containing no sugar.

In order to solve the above problems, the inventors focused on the results of the review and obtained the following findings. That is, it was found that the pineapple extract obtained by solvent extraction of the edible portion of pineapple has excellent laminin-5 production promoting action, epidermal hyaluronic acid production promoting action, hyaluronidase activity inhibiting effect, and branamide The transferase-1 production promotes, melanin production inhibition, and dermal papilla cell proliferation promoting action, so the pineapple extract is suitable as a laminin-5 production promoter, epidermal hyaluronic acid production promoter, hyaluronidase An activity inhibitor, a branamine transferase-1 production promoter, a melanin production inhibitor, a dermal papilla cell proliferation promoter, and a food or drink, a skin external preparation, and a hair growth agent.

The present inventors have made the following points as a means for solving the above problems based on the above findings. which is,

<1> A pineapple extract containing no sugar, which is obtained by extracting an edible portion of an pineapple by a solvent, and does not contain glucose and fructose.

<2> The pineapple extract containing no sugar as described in the above <1>, which is obtained by extracting the residue after pressing of the edible portion of the pineapple by a solvent.

The pineapple extract containing no sugar according to any one of the above <1> to <2>, wherein the solvent is 70% by volume to 100% by volume of an aqueous ethanol solution.

<4> A method for producing a pineapple extract containing no sugar, characterized in that the edible portion of the pineapple is extracted by a solvent to remove glucose and fructose.

<5> The method for producing a pineapple extract containing no sugar according to the above <4>, wherein the residue after pressing of the edible portion of the pineapple is extracted by a solvent.

<6> The method for producing a pineapple extract containing no sugar according to any one of the above <4> to <5> wherein the solvent is a 70% by volume to 100% by volume aqueous solution of ethanol.

<7> A laminin-5 production-promoting agent, which comprises the pineapple extract containing no sugar as described in any one of the above <1> to <3>.

<8> A skin hyaluronic acid production-promoting agent, which comprises the pineapple extract containing no sugar as described in any one of the above <1> to <3>.

<9> A hyaluronan activity inhibitor, which comprises the pineapple extract containing no sugar as described in any one of the above <1> to <3>.

<10> A branamine transferase-1 production-promoting agent, which comprises the pineapple extract containing no sugar as described in any one of the above <1> to <3>.

<11> A melanin production inhibitor, which comprises the pineapple extract containing no sugar as described in any one of the above <1> to <3>.

<12> A porcine lancet cell proliferation promoter, which comprises the pineapple extract containing no sugar as described in any one of the above <1> to <3>.

<13> A food or drink containing the pineapple extract containing no sugar as described in any one of the above <1> to <3>.

<14> A skin external preparation containing the pineapple extract containing no sugar as described in any one of the above <1> to <3>.

<15> A hair growth agent comprising the pineapple extract containing no sugar according to any one of the above <1> to <3>.

According to the present invention, the pineapple extract containing no sugar and the method for producing the same can provide daily neat intake, which is excellent in safety and productivity, and is inexpensive, and provides excellent laminin-5 production promoting action and epidermis. Pineapple extract containing no sugar, at least one of hyaluronic acid production promoting action, hyaluronidase activity inhibiting action, branamine transferase-1 production promoting action, melanin production inhibiting action, and dermal papilla cell proliferation promoting action And its method of manufacture.

According to the laminin-5 production-promoting agent of the present invention, it is possible to provide a laminin-5 production-promoting agent which solves the problems of the past and has excellent laminin-5 production-promoting action and high safety.

According to the epidermal hyaluronic acid production promoter of the present invention, it is possible to provide an epidermal hyaluronic acid production promoter which has a problem of solving the problems of the present invention and which has an excellent epidermal hyaluronic acid production promoting action and high safety.

According to the hyaluronidase activity inhibitor of the present invention, it is possible to provide a hyaluronidase activity inhibitor which solves the problems of the past and has excellent hyaluronidase activity inhibitory action and high safety.

The branamine transferase-1 production promoter according to the present invention can provide the problem of solving the problems, and has excellent branamine transferase-1 production promoting action and high safety of branamine transferase-1 production. Promoter.

According to the melanin production inhibitor of the present invention, it is possible to provide a melanin production inhibitor which solves the problems of the past and has excellent melanin production inhibitory action and high safety.

According to the dermal papilla cell proliferation accelerating agent of the present invention, it is possible to provide a dermal papilla cell proliferation accelerating agent which solves the problems of the past and has excellent black hair papilla cell proliferation action and high safety.

Description of specific examples

(pineapple)

Pineapple is a perennial plant of the genus Bromeliad. The scientific name is Ananas comosus (L.) Merr. or Ananas sativus Schult , also known as pineapple in China. The fruit is large-horned and fleshy, yellow and ripe to give off aroma and can be used as a food. The origin of the pineapple is mainly the United States, the Philippines, Malaysia, Brazil, Australia, etc., but the type or place of production is not particularly limited in obtaining the extract used in the present invention.

(Manufacturing method without sugar pineapple extract sugar and without pineapple extract)

The pineapple extract containing no sugar of the present invention can be obtained by extracting a solvent from an edible portion of pineapple as an extraction raw material. The sugar-free pineapple extract is essential for the absence of glucose and fructose.

The method for producing a pineapple extract containing no sugar of the present invention comprises extracting the edible portion of the pineapple by a solvent to remove glucose and fructose, and further includes other steps which are suitably selected as necessary.

The extracting material of the pineapple extract containing no sugar is not particularly limited as long as it is a pineapple edible portion such as a pulp or a core portion (core), and may be appropriately selected depending on the purpose, but the pineapple is edible. The residue after the partial pressing, that is, the fiber (Plumb pulp) remaining after taking the pineapple juice is particularly preferable.

It is preferred that the above-mentioned extraction raw material is washed, dried, and pulverized by using a juice. Among them, drying can be carried out using daylight or by using a dryer which is usually used.

The pineapple extract containing no sugar can be easily obtained by introducing the pineapple edible portion into the solvent and extracting it at a temperature of from room temperature to the boiling point of the solvent or lower using any apparatus.

The solvent is not particularly limited and may be appropriately selected depending on the purpose. For example, a lower aliphatic alcohol having a carbon number of 1 to 5 such as methanol, ethanol, propanol or isopropanol; acetone or methyl ethyl; a lower aliphatic ketone such as a ketone; a polyvalent alcohol having 2 to 5 carbon atoms such as 1,3-butanediol, propylene glycol or glycerin; hexane; a lower aliphatic alcohol, a lower aliphatic ketone, a polyvalent alcohol or the like a mixed solvent of a hydrophilic organic solvent and water, and the like. Among these, a mixed solvent of a hydrophilic organic solvent and water is preferred. The hydrophilic organic solvent is not particularly limited and may be appropriately selected depending on the intended purpose, but methanol, ethanol, propanol, 1,3-butylene glycol, glycerin, propylene glycol or the like is preferred, and ethanol is more preferred.

In the case of using a lower alcohol as the hydrophilic organic solvent in a mixed solvent of water and a hydrophilic organic solvent, it is preferred that the content of the lower aliphatic alcohol in the mixed solvent is from 10% by volume to 100% by volume. 70% by volume to 100% by volume is more preferable, and 90% by volume is particularly preferable. When a lower aliphatic ketone is used as the hydrophilic organic solvent, the content of the lower aliphatic ketone in the mixed solvent is preferably from 10% by volume to 80% by volume. In the case where a polyvalent alcohol is used as the hydrophilic organic solvent, it is preferred that the content of the polyvalent alcohol in the mixed solvent is from 10% by volume to 90% by volume.

The extraction method of the pineapple extract containing no sugar is not particularly limited as long as the fat-soluble component contained in the edible portion of the pineapple is eluted in the solvent, and can be carried out according to a usual method. Further, in the extraction treatment, it is not necessary to use a special extraction method, and any apparatus can be used from room temperature to reflux heating.

Specifically, the extraction method of the pineapple extract containing no sugar, for example, can be exemplified by the residue of the edible portion of the pineapple (the pineapple meat portion) in a treatment tank filled with the solvent such as an aqueous ethanol solution. The above-mentioned extraction raw material is heated and extracted at 80 ° C for 2 hours with a reflux extractor as necessary, and filtered to elute the fat-soluble component after heating, and then concentrated under reduced pressure using an evaporator. The same filtration treatment is carried out, and the obtained intermediate extract is washed with water, and a method of removing the sugar-free pineapple extract as a target product is obtained by removing saccharides such as glucose and fructose.

In this case, the extraction conditions may be appropriately adjusted depending on the extraction raw material, etc., but the extraction solvent amount is preferably 5 times to 20 times (mass ratio) with respect to the pineapple edible portion as the extraction raw material, and the extraction time is preferably It is preferably 1 hour to 3 hours, and the extraction temperature is preferably from room temperature to 95 ° C.

Further, the obtained sugar-free pineapple extract obtained by the method may be diluted according to a usual method in order to obtain a diluted product, a concentrate, a dried product, a crude purified product, a purified product, and the like of the sugar-free pineapple extract. Concentration, drying, purification, etc.

Further, the obtained sugar-free pineapple extract obtained can also be directly used as a laminin-5 production promoter, epidermal hyaluronic acid production promoter, hyaluronidase activity inhibitor, and branamide transferase- (1) One of a promoter, a melanin production inhibitor, and a dermal papilla cell proliferation promoter, and the concentrate and the dried product are preferably used at the point of easy use. When the dried product is obtained, a carrier such as dextrin or cyclodextrin may be added to improve moisture absorption.

<<Ingredients not included>>

In the present invention, "sugar" which does not contain sugar means glucose and fructose. In addition, the content of the sugar-free pineapple extract is 0.5% by mass or less, and preferably 0.2% by mass or less, in the case of "not containing", "excluding sugar", and "removing sugar".

The content of the aforementioned glucose and fructose can be measured, for example, using a glucose/fructose analysis kit (F-cluster (glucose/fructose), manufactured by J.K. International Co., Ltd.) according to the instructions attached to the kit. Further, as for the sample to be tested, the above-mentioned pineapple extract containing no sugar can be dissolved in a 50% by volume aqueous solution of ethanol to have a sample concentration of 5% by volume.

(Laminin-5 production promoter, epidermal hyaluronic acid production promoter, hyaluronidase activity inhibitor, branamine transferase-1 production promoter, melanin production inhibitor, dermal papilla cell proliferation promoter)

The laminin-5 production promoter of the present invention comprises the above-mentioned pineapple-free extract containing no sugar, and further contains other components as necessary.

The epidermal hyaluronic acid production promoter of the present invention comprises the above-mentioned pineapple extract containing no sugar, and further contains other components as necessary.

The hyaluronidase activity inhibitor of the present invention comprises the above-mentioned pineapple-free extract containing no sugar, and further contains other components as necessary.

The branamine transferase-1 production promoter of the present invention comprises the above-mentioned pineapple extract containing no sugar, and further contains other components as necessary.

The melanin production inhibitor of the present invention comprises the above-mentioned pineapple-free extract containing no sugar, and further contains other components as necessary.

The dermal papilla cell proliferation promoting agent of the present invention comprises the above-mentioned pineapple-free extract containing no sugar, and further contains other components as necessary.

The laminin-5 production promoter of the present invention may contain only the above-mentioned pineapple extract containing no sugar, and may contain other components.

The content of the aforementioned sugar-free pineapple extract in the aforementioned laminin-5 production promoter is not particularly limited and may be appropriately selected depending on the purpose.

The epidermal hyaluronic acid production promoter of the present invention may contain only the above-mentioned pineapple extract containing no sugar, and may contain other components.

The content of the aforementioned sugar-free pineapple extract in the above-mentioned epidermal hyaluronic acid production promoter is not particularly limited, and may be appropriately selected depending on the purpose.

The hyaluronidase activity inhibitor of the present invention may contain only the above-mentioned pineapple extract containing no sugar, and may contain other components.

The content of the aforementioned sugar-free pineapple extract in the above-mentioned hyaluronidase activity inhibitor is not particularly limited, and may be appropriately selected depending on the purpose.

The branamide transferase-1 production promoter of the present invention may contain only the above-mentioned pineapple extract containing no sugar, and may contain other components.

The content of the aforementioned sugar-free pineapple extract in the branamine transferase-1 production promoter is not particularly limited and may be appropriately selected depending on the purpose.

The melanin production inhibitor of the present invention may contain only the above-mentioned pineapple extract containing no sugar, and may contain other components.

The content of the aforementioned sugar-free pineapple extract in the melanin production inhibitor is not particularly limited and may be appropriately selected depending on the purpose.

The dermal papilla cell proliferation promoting agent of the present invention may contain only the above-mentioned pineapple extract containing no sugar, and may contain other components.

The content of the aforementioned sugar-free pineapple extract in the dermal papilla cell proliferation promoter is not particularly limited, and may be appropriately selected depending on the purpose.

<Other ingredients>

The pineapple extract containing no sugar may be prepared into a powder, a granule or a lozenge according to a usual method using any auxiliary agent other than a pharmaceutically acceptable carrier such as dextrin or cyclodextrin. It can be used as any ointment or the like, and can be used as an ointment, an external solution, a patch, or the like, in addition to being used in other compositions (for example, oral pharmaceuticals). At this time, as the adjuvant, for example, an excipient, a binder, a breaker, a lubricant, a stabilizer, a flavoring agent, a flavoring agent, or the like can be used.

As the aforementioned excipient, for example, lactose, white sugar, sodium chloride, glucose, starch, calcium carbonate, kaolin, microcrystalline cellulose, citric acid or the like can be exemplified. Further, as the above-mentioned binder, for example, water, ethanol, propanol, monosaccharide, glucose solution, starch solution, gelatin solution, carboxymethylcellulose, hydroxypropylcellulose, hydroxypropyl starch, and nail can be exemplified. Cellulose, ethyl cellulose, shellac, calcium phosphate, polyvinyl pyrrolidone, and the like. As the above-mentioned breaker, for example, dried starch, sodium alginate, agarose, sodium hydrogencarbonate, calcium carbonate, sodium lauryl sulfate, stearic acid monoglyceride, lactose or the like can be exemplified. As the aforementioned lubricant, for example, talc, stearate, borax, polyethylene glycol, or the like can be exemplified. As the stabilizer, for example, sodium pyrosulfite, EDTA, thioglycolic acid, thiolactic acid or the like can be exemplified. As the flavoring agent and the flavoring agent, for example, white sugar, orange peel, citric acid, tartaric acid or the like can be exemplified.

Further, the laminin-5 production promoter, the epidermal hyaluronic acid production promoter, the hyaluronidase activity inhibitor, the branamine transferase-1 production promoter, the melanin production inhibitor, and the dermal papilla of the present invention Cell proliferation promoter, if necessary, can promote laminin-5 production, epidermal hyaluronic acid production promotion, hyaluronidase activity inhibition, branamine transferase-1 production promotion, melanin production Inhibition, and other natural extracts of any of the dermal papilla cell proliferation promoting effects are blended together.

The laminin-5 production promoting agent exerts a laminin-5 production promoting action by containing the aforementioned sugar-free pineapple extract as an active ingredient.

The epidermal hyaluronic acid production promoter exerts an epidermal hyaluronic acid production promoting action by containing the above-mentioned sugar-free pineapple extract as an active ingredient.

The hyaluronidase activity inhibitor exerts a hyaluronidase activity inhibitory action by containing the above-mentioned sugar-free pineapple extract as an active ingredient.

The branamine transferase-1 production promoter exerts a branamine transferase-1 production-promoting action by containing the aforementioned sugar-free pineapple extract as an active ingredient.

The melanin production inhibitor exerts a melanin production inhibitory action by containing the aforementioned sugar-free pineapple extract as an active ingredient.

The dermal papilla cell proliferation promoting agent exerts a dermal papilla cell proliferation promoting action by containing the aforementioned sugar-free pineapple extract as an active ingredient.

The laminin-5 production promoter of the present invention promotes skin laminin-5 by promoting laminin-5 production, for example, promoting the formation of the basement membrane of the skin, and preventing and improving wrinkles and elasticity. become possible. However, the laminin-5 production promoter of the present invention can be used for all uses having the meaning of promoting the production of laminin-5 in addition to these uses.

The epidermis hyaluronic acid production promoter of the present invention promotes the skin aging symptoms such as the reduction of the moisturizing power and the elasticity by the excellent hyaluronic acid-promoting action, for example, the moisturizing power and the elasticity are improved, and the skin is reduced and the elasticity is reduced. become possible. However, the epidermal hyaluronic acid production promoter of the present invention can be used for all uses having the meaning of promoting the promotion of epidermal hyaluronic acid in addition to these uses.

The hyaluronidase activity inhibitor of the present invention inhibits hyaluronic acid activity by, for example, inhibits decomposition of hyaluronic acid, promotes release of histamine, enhances moisturizing, prevents and improves inflammation or allergy. may. However, the hyaluronidase activity inhibitor of the present invention can be used for all uses having the meaning of exhibiting the activity of inhibiting hyaluronidase activity in addition to these uses.

The branamine transferase-1 production promoter of the present invention promotes the production of the branamine transferase-1 by an excellent branamine transferase-1, for example, the barrier function of the skin epidermis, the disappearance of rough skin texture, prevention and improvement of elasticity The aging symptoms such as lowering are possible. However, the branamine transferase-1 production promoter of the present invention can be used for all uses having the meaning of promoting the production of branamine transferase-1 in addition to these uses.

The melanin production inhibitor of the present invention is produced by an excellent melanin production inhibitory action, for example, it is possible to protect the skin from ultraviolet rays, prevent and improve the aging symptoms of skin blackening, spots, and the like. However, the melanin production inhibitor of the present invention can be used for all uses having the meaning of exhibiting a melanin production inhibitory effect in addition to these uses.

According to the dermal papilla cell proliferation promoter of the present invention, it is possible to promote the proliferation of hairy epithelial cells, the differentiation of hair, the prevention, and the improvement of the bristles through the promotion of the dermal papilla cell proliferation promoting action. In addition to these uses, the dermal papilla cell proliferation promoter of the present invention can be used for all uses having the meaning of promoting the proliferation of dermal papilla cells.

The laminin-5 production promoter, the epidermal hyaluronic acid production promoter, the hyaluronidase activity inhibitor, the branamine transferase-1 production promoter, the melanin production inhibitor, and the dermal papilla cell proliferation of the present invention The accelerator has an excellent effect, and the extract obtained from the edible pineapple is excellent in safety, so it is suitable for blending with foods and drinks, external preparations for skin, and hair growth agents.

Further, the laminin-5 production promoter, the epidermal hyaluronic acid production promoter, the hyaluronidase activity inhibitor, the branamine transferase-1 production promoter, the melanin production inhibitor, and the dermal papilla of the present invention Since the cell proliferation promoter has an excellent effect, it can be suitably used as a test for laminin-5, epidermal hyaluronic acid, hyaluronidase, branamine transferase-1, and dermal papilla cells. Drugs; reagents for the study of functions and diseases related to hyaluronidase, laminin-5, epidermal hyaluronic acid, branamine transferase-1, melanin, and dermal papilla cells.

Further, the laminin-5 production promoter, the epidermal hyaluronic acid production promoter, the hyaluronidase activity inhibitor, the branamine transferase-1 production promoter, the melanin production inhibitor, and the dermal papilla of the present invention The cell proliferation promoting agent can be suitably applied to humans, but can be used for animals other than humans as long as it can exert various effects.

(food and drink)

The food and drink of the present invention contains the sugar-free pineapple extract of the present invention, a laminin-5 production promoter, an epidermal hyaluronic acid production promoter, a hyaluronidase activity inhibitor, and a branamide transferase. -1 is produced by at least one of a promoter, a melanin production inhibitor, and a dermal papilla cell proliferation promoter, and further contains other components as necessary.

In the above-mentioned food and beverage system, the above-mentioned food-free product containing the sugar-free pineapple extract may be blended into any food or drink that does not interfere with the activity, and may be a food or drink containing the sugar-free pineapple extract as a main component. Further, the food or drink may be the aforementioned pineapple extract containing no sugar.

The above-mentioned food and drink products have few doubts about the harm to human health. In the ordinary social life, the so-called oral or digestive tract administration of the ingested person is not limited by the administrative division of food, medicine, medical supplies, etc. The distinction means, for example, a wide range including general foods, health foods, health functional foods, beauty foods, medical supplies, and pharmaceuticals which are orally ingested.

Further, the food or beverage of the present invention is processed into a shape such as a drink, a hard capsule, a soft capsule, or a granule by a form, and can be easily eaten and used, and may be used in a wide range.

The foods and drinks are not particularly limited, and may be appropriately selected depending on the purpose, for example, beverages such as refreshing drinks, carbonated drinks, nutritional drinks, fruit drinks, and lactic acid drinks (including concentrates and adjustment powders of such drinks) ); cold desserts such as ice cream, sherbet, shaved ice; soba noodles, oolong noodles, hollow noodles, dumplings, siu mai, Chinese noodles, instant noodles, etc.; sugar, candy, chewing gum, Desserts such as chocolate, ingots, Japanese snacks, biscuits, pectin, jellies, creams, baked biscuits, bread, etc.; crabs, squid, clams, squid, sardines, shrimps, flying fish, squid, whales, oysters, Water products of saury, squid, scallop, scallop, abalone, sea urchin, caviar, Japanese abalone; aquatic products such as fish plate, ham, sausage, ‧ livestock processed food; processed milk, fermented milk, etc.; salad oil, Oils and fat processed foods such as tempura oil, margarine, mayonnaise, shortening, whipping cream, dressing, etc.; sauce, Seasonings such as roast sauce; curry, stew, parent-child porridge, porridge, muesli, Chinese sturgeon, pork chop, scorpion, squid, beef risotto, yoda, maho tofu, sirloin, bolognese, egg flower Soups, omelettes, dumplings, siu mai, burgers, meatballs, etc. (retort pouch); salads, pickles and other non-staple foods (chives); various forms of health, beauty, nutritional supplements; lozenges, granules, Capsules, beverages, troche-containing pharmaceuticals, medical supplies, and the like. Moreover, the above-mentioned foods and drinks are not limited to the above-mentioned examples.

The aforementioned sugar-free pineapple extract, laminin-5 production promoter, epidermal hyaluronic acid production promoter, hyaluronidase activity inhibitor, and branamine transferase-1 production promotion in the above-mentioned foods and drinks The content of any of the agent, the melanin production inhibitor, and the dermal papilla cell proliferation promoter varies depending on the food or drink to be added, and cannot be uniformly defined, but the case of a tablet or a capsule is converted. The amount of the pineapple extract containing no sugar is preferably 1% by mass to 90% by mass, and in other foods and drinks, the amount of the pineapple extract containing no sugar is 0.001% by mass to 50% by mass. Preferably. In addition, it is preferable to adjust the amount of intake of the edible pineapple extract containing no sugar to about 1 mg to 1,000 mg per day for an adult.

The other components mentioned above can be exemplified as auxiliary materials, additives, and the like which are usually used in the production of the aforementioned foods and drinks.

The auxiliary materials and additives are not particularly limited and may be appropriately selected depending on the purpose, and examples thereof include glucose, fructose, sucrose, maltose, sorbitol, stevioside, and rubusoside. Corn syrup, lactose, citric acid, tartaric acid, malic acid, succinic acid, lactic acid, L-ascorbic acid, dl-α-tocopherol, sodium erythorbate, glycerin, propylene glycol, glycerol fatty acid ester, polyglycerol fatty acid Ester, sucrose fatty acid ester, sorbitan fatty acid ester, arabic gum, carrageenan, casein, gelatin, pectin , agar, vitamin B, niacin amide, calcium pantothenate, amino acids, calcium salts, pigments, spices, preservatives, etc.

The food or drink of the present invention can be orally ingested daily, and the hyaluronidase activity inhibiting action and the laminin-5 production promoting action can be extremely effectively achieved by the action of the pineapple extract as an active ingredient. At least one of a hyaluronic acid production promoting action, a branamine transferase-1 production promoting action, and a dermal papilla cell proliferation promoting action.

Further, the food or drink of the present invention is suitable for a human being, but it can be applied to animals other than humans as long as it can produce individual effects.

(skin external preparation)

The external preparation for skin of the present invention comprises the pineapple extract containing no sugar of the present invention, a laminin-5 production promoter, an epidermal hyaluronic acid production promoter, a hyaluronidase activity inhibitor, and a glutamine transfer Any one of an enzyme-1 production promoter, a melanin production inhibitor, and a dermal papilla cell proliferation promoter, and further contains other components as necessary.

Here, the above-mentioned skin external preparation means various medicines used for the skin, and the distinction is not particularly limited, and for example, it includes a wide range of skin cosmetics, medical external products, pharmaceuticals, and the like.

The skin external preparation may be a skin external preparation containing the sugar-free pineapple extract as a main component, without interfering with the activity, by blending the sugar-free pineapple extract with any skin external preparation. . Further, the skin external preparation may be the aforementioned pineapple extract containing no sugar.

The skin external preparation is not particularly limited and may be appropriately selected depending on the purpose, and examples thereof include ointments, creams, lotions, lotions, lotions, masks, foundations, lipsticks, bathing agents, hair restorers (hair). Tonic), hair lotion, soap, shower gel, etc.

The other components are not particularly limited, and components which are usually used for the preparation of the external preparation for skin can be appropriately selected depending on the purpose, and examples thereof include astringents, sterilization, antibacterial agents, whitening agents, ultraviolet absorbers, moisturizers, and the like. Cell activators, anti-aging agents, anti-inflammatory, anti-allergic agents, antioxidants, active oxygen removers, oils and fats, waxes, hydrocarbons, fatty acids, alcohols, esters, surfactants, perfumes, etc.

The aforementioned sugar-free pineapple extract, laminin-5 production promoter, epidermal hyaluronic acid production promoter, hyaluronidase activity inhibitor, and branamide transferase-1 in the skin external preparation The content of the accelerator, the melanin production inhibitor, and the dermal papilla cell proliferation promoter is not particularly limited, and may be appropriately selected depending on the type of the external preparation for skin, for example, the above-mentioned sugar-free substance. The amount of the pineapple extract is preferably 0.0001% by mass to 10% by mass, more preferably 0.001% by mass to 5% by mass.

(hair growth agent)

The hair growth agent of the present invention comprises the pineapple extract containing no sugar of the present invention, a laminin-5 production promoter, an epidermal hyaluronic acid production promoter, a hyaluronidase activity inhibitor, and a glutamine transfer Any one of an enzyme-1 production promoter, a melanin production inhibitor, and a dermal papilla cell proliferation promoter, and further contains other components as necessary.

The hair growth agent may be a hair growth agent containing the sugar-free pineapple extract as a main component, without interfering with the activity thereof, by blending the sugar-free pineapple extract with any hair growth agent. . Further, the hair growth agent may be the aforementioned pineapple extract containing no sugar.

The hair growth agent of the present invention promotes laminin-5 production promotion, epidermal hyaluronic acid production promotion, hyaluronidase activity inhibition, branamine transferase-1 production promotion, melanin production For example, it can promote the proliferation of the papillary cells and the hairpin epithelial cells, the differentiation of the hair, and the like, and can be suitably used for preventing and improving the finening and hair removal.

In addition, the "hair growth agent" in the present specification means a drug having any effect of developing hair, promoting hair development, maintaining a healthy state of hair, preventing hair loss, and reducing hair loss, and also includes a "hair raising agent". Therefore, the hair growth agent of the present invention can also be preferably used as a hair growth agent.

Since the hair growth agent of the present invention has an excellent effect and is excellent in the feeling of use and safety in the case of being applied to the skin, it is suitable for a skin external preparation such as a hair growth agent for hair or a hair cosmetic. In the case of the administration site of the skin external preparation, for example, the head of the hair, the hair root of the hair, the scalp, the sebaceous gland of the scalp, or the like is preferable. The dosage form of the aforementioned external preparation for skin may, for example, be an ointment, a cream, a lotion, a gel, a sol, a spray, a solution or the like. As the hair cosmetic, for example, a hair tonic, a hair liquid, a shampoo, a hair oil, a conditioner, and the like can be exemplified.

The aforementioned sugar-free pineapple extract, laminin-5 production promoter, epidermal hyaluronic acid production promoter, hyaluronidase activity inhibitor, and branamide transferase-1 in the hair growth agent The content of the accelerator, the melanin production inhibitor, and the dermal papilla cell proliferation promoter is not particularly limited, and may be appropriately selected depending on the type of the hair growth agent, etc., but for example, the above-mentioned sugar-free substance is converted. The amount of the pineapple extract is preferably 0.0001% by mass to 10% by mass, more preferably 0.001% by mass to 5% by mass.

Example

Hereinafter, the present invention will be more specifically described by showing production examples and test examples, but the present invention is not limited thereto.

(Production Example 1: Production of pineapple extract containing no sugar)

The crushed residue (pineapple fleshy portion) of 100 g of pineapple edible portion was added to 1,000 ml of 90% by volume of ethanol, and extracted by heating at 80 ° C for 2 hours in a reflux extractor, and filtered while heating. Thereafter, it was concentrated under reduced pressure using an evaporator, and the same filtration treatment was carried out. The obtained residue was washed with 500 ml of water to obtain 1.5 g of a sugar-free paste-like pineapple-free extract. The yield of the extract was 1.5 (% by mass).

The obtained pineapple extract containing no sugar was subjected to the following composition analysis.

The above-mentioned sugar-free pineapple extract was dissolved in 50% by volume of ethanol to have a sample concentration of 20% by volume as a sample to be tested, and the glucose/fructose analysis kit (F-set (glucose/fructose), JK International) was used. Co., Ltd.) measures the content of glucose and fructose. As a result of the measurement, it was found that the pineapple extract containing no sugar contained 0.03 mass% of glucose and 0.01 mass% of fructose.

The pineapple extract containing no sugar obtained in the above-mentioned Production Example 1 was used as a test sample, and the skin, whitening and hair growth of the pineapple extract containing no sugar were carried out as shown in the following Test Examples 1 to 6. Evaluation.

In addition, as a control pineapple extract, a freeze-dried product of 26.3 g of pineapple juice obtained by immersing 100 ml of 100% pineapple juice (commercial pineapple juice) in a reduced pressure using an evaporator under reduced pressure was used. The result of measuring the content of glucose and fructose in the same manner as the pineapple extract without sugar was as follows, and the result was that the pineapple juice lyophilized product contained 29.1% by mass of glucose, 26.6. Quality % fructose.

(Test Example 1: Test for promoting adhesion of laminin-5)

<Test method>

Human normal neonatal skin epidermal keratinocytes (NHEK, manufactured by Kurabo Industries, Ltd.) were cultured using human normal neonatal epidermal keratinocyte culture medium (KGM, manufactured by Kurabo Industries Co., Ltd.) via trypsin ( Trypsin) treatment to recover cells. The cells diluted and purified by KGM (KGM-BPE, manufactured by Kurabo Industries, Ltd.) without added BPE were brought to a concentration of 1 × 10 5 cells/mL, and then 500 μL of each well was perforated in a 24-well plate and cultured for 2 days. After the completion of the culture, the medium was taken out, and 500 μL of the test sample dissolved in KGM-BPE was added to each well, and cultured for 48 hours. After the end of the culture, 100 μL of the supernatant was transferred to an ELISA plate, and after adsorption to a flat plate at 37 ° C for 2 hours, the solution was removed to a physiological buffer solution of 0.05% by volume of Tween 20 (0.05% PBS-T, Wash at pH 7.4).

Thereafter, a blocking operation was carried out using a physiological phosphate buffer (pH 7.4) containing 1% by volume of bovine serum albumin. The solution was removed and washed with 0.05% PBS-T to make anti-human laminin-5 antibody (mouse IgG, Japan) Co., Ltd.) reaction. The solution was removed and washed with 0.05% PBS-T to carry out a color reaction. From the obtained measurement results, the laminin-5 production promotion rate (%) was calculated from the following formula.

The calculation method for laminin-5 production promoting effect is as follows. The results are shown in Table 1.

Laminin-5 production promotion rate (%)=A/B×100‧‧‧(1)

[However, in the above formula (1), A and B respectively indicate

A: Absorbance at a wavelength of 405 nm when the test sample is added

B: Absorbance at a wavelength of 405 nm when no test sample was added (control group).

From the results of Table 1, it is considered that the pineapple extract containing no sugar has a concentration-dependent laminin-5 production promoting effect.

(Test Example 2: Test for promoting the production of epidermal hyaluronic acid)

<Test method>

Human normal neonatal skin epidermal keratinocytes (NHEK) were cultured using human normal neonatal epidermal keratinocytes culture medium (KGM), and the cells were recovered by trypsin treatment. The recovered cells were diluted with KGM to a concentration of 1 × 10 5 cells/mL, and then 100 μL of each well was incubated in a 96-well plate for 24 hours. After the completion of the culture, 100 μL of the test sample dissolved in KGM was added to each well, and culture was carried out for 7 days. After the culture, the amount of hyaluronic acid in the medium of each well was measured using a sandwich method of hyaluronic acid-binding protein (HABP, manufactured by Biochemical Biobusiness Co., Ltd.).

The calculation method for promoting hyaluronic acid production is as follows. The results are shown in Table 2.

Hyaluronic acid production promotion rate per % (%) = A / B × 100‧‧‧ (2)

[However, in the above formula (2), A and B represent

A: The amount of hyaluronic acid added when the test sample is added

B: No amount of hyaluronic acid when the test sample was added].

From the results of Table 2, it is considered that the pineapple extract containing no sugar has a significance for promoting epidermal hyaluronic acid production.

(Test Example 3: Test for inhibition of hyaluronidase activity)

<Test method>

0.1 mL of hyaluronidase solution (Type IV-S (from burdock), 400 NF units/mL; Sigma-Aldrich Japan was added to 0.2 mL of 0.1 mol/L acetate buffer (pH 3.5) in which the test sample was dissolved. The reaction was carried out at 37 ° C for 20 minutes. Further, 0.2 mL of 2.5 mmol/L calcium chloride as an activating agent was added and reacted at 37 ° C for 20 minutes. 0.5 mL of a 0.4 mg/mL sodium hyaluronate solution (sodium hyaluronate from avian chicken crown, manufactured by Wako Pure Chemical Industries, Ltd.) was added thereto, and reacted at 37 ° C for 40 minutes. Thereafter, 0.2 mL of 0.4 mol/L sodium hydroxide was added, the reaction was terminated, and after cooling, 0.2 mL of a boric acid solution was added to each reaction solution, followed by boiling for 3 minutes. After ice-cooling, 6 mL of a p-DABA reagent (p-dimethylaminobenzaldehyde, manufactured by Wako Pure Chemical Industries, Ltd.) was added, and the mixture was reacted at 37 ° C for 20 minutes. Thereafter, the absorbance at a wavelength of 585 nm was measured. The blank test was carried out in the same way to be corrected.

The calculation method for the inhibition of hyaluronidase activity is as follows. The results are shown in Table 3.

Hyaluronidase activity inhibition rate (%)={1-(St-Sb)/(Ct-Cb)}×100 ‧‧‧式(3)

[However, in the above formula (3), St, Sb, Ct, and Cb are each represented by

St: absorbance at a wavelength of 585 nm of the test solution

Sb: absorbance at a wavelength of 585 nm in the blank group of the test solution

Ct: absorbance in the control solution at a wavelength of 585 nm

Cb: absorbance at a wavelength of 585 nm in the blank group of the control solution].

From the results of Table 3, it is considered that the pineapple extract containing no sugar has an inhibitory effect on hyaluronidase activity. Further, the dried product of the juice extracted from the pineapple is conversely considered to have an effect of promoting hyaluronidase activity.

(Test Example 4: Test for the production of branamine transferase-1)

<Test method>

Human normal neonatal skin epidermal keratinocytes (NHEK) were cultured using human normal neonatal epidermal keratinocytes culture medium (KGM), and the cells were recovered by trypsin treatment. The recovered cells were diluted with KGM to a concentration of 1 × 10 5 cells/mL, and then 100 μL of each of the cells in a 96-well plate was inoculated for 2 days. After the completion of the culture, 100 μL of the test sample dissolved in KGM was added to each well and cultured for 24 hours. After the culture, the medium was removed, and a single anti-human glutamine transferase-1 antibody (Anti-Keratinocyte Transglutaminase, manufactured by Biomedical Technologies, Inc.) was used to measure the expression of the cells immobilized on the surface of the cells on the flat plate by ELISA. The amount of branamine transferase-1.

The calculation method for the production of branamine transferase-1 is as follows. The results are shown in Table 4.

Brassamine transferase-1 production promotion rate (%)=A/B×100‧‧‧(4)

[However, in the above formula (4), A and B respectively indicate

A: Absorbance at a wavelength of 405 nm when the test sample is added

B: Absorbance at a wavelength of 405 nm when no test sample was added (control group).

From the results of Table 4, it was considered that the pineapple extract containing no sugar had a significant branamine transferase-1 production promoting effect.

(Test Example 5: Melanin production inhibition test)

<Test method>

B16 melanoma cells were cultured using Dulbecco's MEM (Dulbecco's modified Eagle medium (1), manufactured by Hyaba Pharmaceutical Co., Ltd.) containing 10% by volume of FBS (standard fetal calf serum (STANDARD FETAL BOVINE SERUM), manufactured by HyClone). The cells are recovered by trypsin treatment. The recovered cells were diluted to a concentration of 24.0×10 4 cells/mL in Dulbecco's MEM containing 10% by volume of FBS and 1 mmol/L of theophylline (Theophylline, manufactured by Wako Pure Chemical Industries, Ltd.), and then dried in a 48-well plate. The wells were each inoculated with 300 μL and cultured for 6 hours. After the completion of the culture, 300 μL of the test sample dissolved in Dulbecco's MEM containing 10% by volume of FBS and 1 mmol/L of theophylline was added to each well and cultured for 4 days. After the completion of the culture, the medium was removed from each well, and 200 μL of a 2 mol/L NaOH solution was added thereto, and the cells were disrupted by an ultrasonic breaker, and the absorbance at a wavelength of 475 nm was measured as a melanin production amount.

Further, in order to measure the cell survival rate, after the culture, the cells were washed with 400 μL of PBS(-) phosphate physiological buffer, and added to each well at a final concentration of 0.05 mg/mL in Dulbecco's MEM containing 10% by volume of FBS. 13.8 mmol / L neutral red 200 μL. After culturing for 2.5 hours, the neutral red solution was discarded, and 200 μL of ethanol ‧ acetic acid solution (ethanol: acetic acid: water = 50:1:49) was added to each well to extract a pigment. After the extraction, the absorbance at a wavelength of 540 nm was measured.

As a blank test, Dulbecco's MEM cultured cells containing only 10% by volume of FBS and 1 mmol/L of theophylline were tested in the same manner.

The calculation method for the inhibition of melanin production is as follows. The results are shown in Table 5.

Melanin production inhibition rate (%) = {1 - (B / D) / (A / C)} × 100 ‧ ‧ ‧ (5)

[However, in the above formula (5), each of A, B, C, and D represents

A: Absorbance of cells without the test sample at a wavelength of 475 nm

B: absorbance of cells added to the test sample at a wavelength of 475 nm

C: Absorbance of cells without the test sample at a wavelength of 540 nm

D: absorbance of the cells to be tested at a wavelength of 540 nm].

From the results of Table 5, it was considered that the pineapple extract containing no sugar had a stronger and concentration-dependent melanin production inhibitory effect than the control pineapple juice lyophilized product.

(Test Example 6: Test for promoting proliferation of dermal papilla cells)

<Test method>

Normal human hair dermal papilla cells (manufactured by Toyobo Co., Ltd.) were cultured using dermal papilla cell proliferation medium (manufactured by Toyobo Co., Ltd.), and the cells were recovered by trypsin treatment. The recovered cells were diluted to a concentration of 1.0 × 10 4 cells/mL using Dulbecco's MEM (Dulbecco's modified Eagle medium (1), manufactured by Nippon Pharmaceutical Co., Ltd.) containing 10% by volume of FBS, and 96-well flat coated with collagen fibers. 200 μL of each well was inoculated and cultured for 3 days. After the culture, the medium was removed, and 200 μL of the test sample dissolved in serum-free DMEM (Dulbecco's modified Eagle medium (1), manufactured by Nippon Pharmaceutical Co., Ltd.) was added to each well, and culture was further carried out for 4 days. The dermal papilla cell proliferation was measured using MTT assay. After the end of the culture, the medium was removed, and 3-(4,5-dimethyl-thiazol-2-yl)-2 bromide dissolved in serum-free DMEM at a final concentration of 0.4 mg/mL was added to each well. 5-diphenyltetrazolium (MTT, manufactured by Tongren Chemical Research Co., Ltd.) 100 μL. After 2 hours of culture, blue formazan produced in the cells was extracted with 100 μL of 2-propanol. After the extraction, the absorbance at a wavelength of 570 nm (OD570) was measured. At the same time, the absorbance (OD650) in the wavelength of turbidity of 650 nm was measured, and the difference between the two was taken as the amount of blue formazan formation.

The calculation method for promoting the proliferation of dermal papilla cells is as follows. The results are shown in Table 6.

Proliferation rate of dermal papilla cells (%)=A/B×100‧‧‧(6)

[However, in the above formula (6), A and B respectively represent

A: OD570-OD650 when the test sample is added

B: OD570-OD650] without adding the test sample.

From the results of Table 6, it was confirmed that only the pineapple extract containing no sugar has a dermal papilla cell proliferation promoting effect.

Industrial availability

The sugar-free pineapple extract, the laminin-5 production promoter, the epidermal hyaluronic acid production promoter, the hyaluronidase activity inhibitor, the branamine transferase-1 production promoter, and the melanin production of the present invention The inhibitor and the dermal papilla cell proliferation promoter are excellent in safety and productivity, are daily ingestible, and are inexpensive, and have excellent laminin-5 production promoting action, epidermal hyaluronic acid production promoting action, and hyaluronicity. At least one of the effects of inhibition of acid enzyme activity, promotion of production of branamine transferase-1, inhibition of melanin production, and promotion of proliferation of dermal papilla cells, so it can be blended into cosmetic foods and products, and blended into the skin for external use. The agent and the hair growth agent can be suitably used as a reagent for research.

Claims (15)

A pineapple extract containing no sugar, which is characterized in that the edible portion of the pineapple is extracted by a solvent, and after concentration, the residue obtained by the filtration treatment is washed with water, and does not contain glucose and fructose. The pineapple extract containing no sugar as described in the first paragraph of the patent application is obtained by extracting the residue after pressing of the edible portion of the pineapple by a solvent. The pineapple extract containing no sugar as described in claim 1 or 2, wherein the solvent is 70% by volume to 100% by volume of an aqueous ethanol solution. A method for producing a pineapple extract containing no sugar, characterized in that the edible portion of the pineapple is extracted by a solvent, and after concentration, the residue obtained by the filtration treatment is washed with water to remove glucose and fructose. A method for producing a pineapple extract containing no sugar as described in claim 4, wherein the residue after pressing of the edible portion of the pineapple is extracted by a solvent. A method for producing a pineapple extract containing no sugar as described in claim 4 or 5, wherein the solvent is a 70% by volume to 100% by volume aqueous solution of ethanol. A laminin-5 production promoting agent comprising a pineapple extract containing no sugar as described in claim 1 or 2. An epidermal hyaluronic acid production promoter comprising a pineapple extract containing no sugar as described in claim 1 or 2. A hyaluronidase activity inhibitor comprising a pineapple extract containing no sugar as described in claim 1 or 2. A branamine transferase-1 production promoter comprising a pineapple extract containing no sugar as described in claim 1 or 2. A melanin production inhibitor characterized by containing a pineapple extract containing no sugar as described in claim 1 or 2. A dermal papilla cell proliferation promoter comprising a pineapple extract containing no sugar as described in claim 1 or 2. A food or drink characterized by containing a pineapple extract containing no sugar as described in the first or second aspect of the patent application. A skin external preparation comprising a pineapple extract containing no sugar as described in claim 1 or 2. A hair growth agent comprising a pineapple extract containing no sugar as described in claim 1 or 2.