TWI670004B - 用來產生植物之螢光激活細胞分選富增技術 - Google Patents

用來產生植物之螢光激活細胞分選富增技術 Download PDF

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TWI670004B
TWI670004B TW102132254A TW102132254A TWI670004B TW I670004 B TWI670004 B TW I670004B TW 102132254 A TW102132254 A TW 102132254A TW 102132254 A TW102132254 A TW 102132254A TW I670004 B TWI670004 B TW I670004B
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dna
artificial sequence
plant
sequence
gene
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TW102132254A
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TW201424578A (zh
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吉爾曼 史賓根堡
薩瑞納 塞哈卜
約翰 梅森
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美商陶氏農業科學公司
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8209—Selection, visualisation of transformants, reporter constructs, e.g. antibiotic resistance markers
    • C12N15/821—Non-antibiotic resistance markers, e.g. morphogenetic, metabolic markers
    • C12N15/8212—Colour markers, e.g. beta-glucoronidase [GUS], green fluorescent protein [GFP], carotenoid
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8213—Targeted insertion of genes into the plant genome by homologous recombination

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Cell Biology (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Botany (AREA)
  • Physiology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Environmental Sciences (AREA)
  • Dispersion Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
TW102132254A 2012-09-07 2013-09-06 用來產生植物之螢光激活細胞分選富增技術 TWI670004B (zh)

Applications Claiming Priority (2)

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US201261697890P 2012-09-07 2012-09-07
US61/697,890 2012-09-07

Publications (2)

Publication Number Publication Date
TW201424578A TW201424578A (zh) 2014-07-01
TWI670004B true TWI670004B (zh) 2019-09-01

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TW102132254A TWI670004B (zh) 2012-09-07 2013-09-06 用來產生植物之螢光激活細胞分選富增技術

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Country Link
US (1) US20140075593A1 (enExample)
EP (1) EP2893024B1 (enExample)
JP (1) JP6362600B2 (enExample)
KR (1) KR102219621B1 (enExample)
CN (2) CN104736712A (enExample)
AR (1) AR092482A1 (enExample)
AU (1) AU2013312198B2 (enExample)
BR (1) BR112015004864B8 (enExample)
CA (1) CA2883800C (enExample)
HK (1) HK1210220A1 (enExample)
IL (1) IL237535B (enExample)
IN (1) IN2015DN01785A (enExample)
MX (1) MX367170B (enExample)
RU (1) RU2679510C2 (enExample)
TW (1) TWI670004B (enExample)
WO (1) WO2014039970A1 (enExample)
ZA (1) ZA201501736B (enExample)

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US9163284B2 (en) 2013-08-09 2015-10-20 President And Fellows Of Harvard College Methods for identifying a target site of a Cas9 nuclease
US9322037B2 (en) 2013-09-06 2016-04-26 President And Fellows Of Harvard College Cas9-FokI fusion proteins and uses thereof
US9526784B2 (en) 2013-09-06 2016-12-27 President And Fellows Of Harvard College Delivery system for functional nucleases
US9340799B2 (en) 2013-09-06 2016-05-17 President And Fellows Of Harvard College MRNA-sensing switchable gRNAs
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US20150166984A1 (en) 2013-12-12 2015-06-18 President And Fellows Of Harvard College Methods for correcting alpha-antitrypsin point mutations
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WO2014039970A9 (en) 2014-10-09
IN2015DN01785A (enExample) 2015-05-29
EP2893024A1 (en) 2015-07-15
JP2015527085A (ja) 2015-09-17
BR112015004864B1 (pt) 2022-10-25
RU2015112573A (ru) 2016-10-27
CN111118100A (zh) 2020-05-08
IL237535B (en) 2019-11-28
ZA201501736B (en) 2016-10-26
MX2015002964A (es) 2015-06-05
CA2883800A1 (en) 2014-03-13
BR112015004864B8 (pt) 2023-05-16
AR092482A1 (es) 2015-04-22
WO2014039970A1 (en) 2014-03-13
US20140075593A1 (en) 2014-03-13
AU2013312198A1 (en) 2015-03-19
CN104736712A (zh) 2015-06-24
KR20150052241A (ko) 2015-05-13
JP6362600B2 (ja) 2018-07-25
EP2893024A4 (en) 2016-03-30
RU2679510C2 (ru) 2019-02-11
MX367170B (es) 2019-08-07
AU2013312198B2 (en) 2018-11-08
EP2893024B1 (en) 2019-12-04
BR112015004864A2 (pt) 2017-11-21
TW201424578A (zh) 2014-07-01
IL237535A0 (en) 2015-04-30
KR102219621B1 (ko) 2021-02-24
CA2883800C (en) 2021-07-13
HK1210220A1 (en) 2016-04-15

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