KR102219621B1 - 식물 생성을 위한 형광 활성화 세포 분류 (facs) 강화 - Google Patents

식물 생성을 위한 형광 활성화 세포 분류 (facs) 강화 Download PDF

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KR102219621B1
KR102219621B1 KR1020157008557A KR20157008557A KR102219621B1 KR 102219621 B1 KR102219621 B1 KR 102219621B1 KR 1020157008557 A KR1020157008557 A KR 1020157008557A KR 20157008557 A KR20157008557 A KR 20157008557A KR 102219621 B1 KR102219621 B1 KR 102219621B1
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plant
sequence
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gene
protoplast
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KR20150052241A (ko
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저먼 스판젠버그
사리나 사하브
존 메이슨
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다우 아그로사이언시즈 엘엘씨
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8213—Targeted insertion of genes into the plant genome by homologous recombination
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H5/00—Angiosperms, i.e. flowering plants, characterised by their plant parts; Angiosperms characterised otherwise than by their botanic taxonomy
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8209—Selection, visualisation of transformants, reporter constructs, e.g. antibiotic resistance markers
    • C12N15/821—Non-antibiotic resistance markers, e.g. morphogenetic, metabolic markers
    • C12N15/8212—Colour markers, e.g. beta-glucoronidase [GUS], green fluorescent protein [GFP], carotenoid
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8214—Plastid transformation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Cell Biology (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Developmental Biology & Embryology (AREA)
  • Botany (AREA)
  • Environmental Sciences (AREA)
  • Dispersion Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Physiology (AREA)
KR1020157008557A 2012-09-07 2013-09-09 식물 생성을 위한 형광 활성화 세포 분류 (facs) 강화 Expired - Fee Related KR102219621B1 (ko)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US201261697890P 2012-09-07 2012-09-07
US61/697,890 2012-09-07
US14/020,694 2013-09-06
US14/020,694 US20140075593A1 (en) 2012-09-07 2013-09-06 Fluorescence activated cell sorting (facs) enrichment to generate plants
PCT/US2013/058766 WO2014039970A1 (en) 2012-09-07 2013-09-09 Fluorescence activated cell sorting (facs) enrichment to generate plants

Publications (2)

Publication Number Publication Date
KR20150052241A KR20150052241A (ko) 2015-05-13
KR102219621B1 true KR102219621B1 (ko) 2021-02-24

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US (1) US20140075593A1 (enExample)
EP (1) EP2893024B1 (enExample)
JP (1) JP6362600B2 (enExample)
KR (1) KR102219621B1 (enExample)
CN (2) CN104736712A (enExample)
AR (1) AR092482A1 (enExample)
AU (1) AU2013312198B2 (enExample)
BR (1) BR112015004864B8 (enExample)
CA (1) CA2883800C (enExample)
HK (1) HK1210220A1 (enExample)
IL (1) IL237535B (enExample)
IN (1) IN2015DN01785A (enExample)
MX (1) MX367170B (enExample)
RU (1) RU2679510C2 (enExample)
TW (1) TWI670004B (enExample)
WO (1) WO2014039970A1 (enExample)
ZA (1) ZA201501736B (enExample)

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JP6362600B2 (ja) 2018-07-25
CN104736712A (zh) 2015-06-24
ZA201501736B (en) 2016-10-26
BR112015004864B1 (pt) 2022-10-25
AR092482A1 (es) 2015-04-22
AU2013312198A1 (en) 2015-03-19
BR112015004864B8 (pt) 2023-05-16
CN111118100A (zh) 2020-05-08
US20140075593A1 (en) 2014-03-13
BR112015004864A2 (pt) 2017-11-21
HK1210220A1 (en) 2016-04-15
IL237535A0 (en) 2015-04-30
IL237535B (en) 2019-11-28
WO2014039970A1 (en) 2014-03-13
EP2893024B1 (en) 2019-12-04
CA2883800C (en) 2021-07-13
CA2883800A1 (en) 2014-03-13
AU2013312198B2 (en) 2018-11-08
EP2893024A1 (en) 2015-07-15
RU2015112573A (ru) 2016-10-27
JP2015527085A (ja) 2015-09-17
TW201424578A (zh) 2014-07-01
IN2015DN01785A (enExample) 2015-05-29
EP2893024A4 (en) 2016-03-30
MX2015002964A (es) 2015-06-05
MX367170B (es) 2019-08-07
RU2679510C2 (ru) 2019-02-11
WO2014039970A9 (en) 2014-10-09
KR20150052241A (ko) 2015-05-13
TWI670004B (zh) 2019-09-01

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