TWI636136B - 在酵母菌及其它轉型細胞中多肽高產量表現用之溫度變動技術 - Google Patents
在酵母菌及其它轉型細胞中多肽高產量表現用之溫度變動技術 Download PDFInfo
- Publication number
- TWI636136B TWI636136B TW103109931A TW103109931A TWI636136B TW I636136 B TWI636136 B TW I636136B TW 103109931 A TW103109931 A TW 103109931A TW 103109931 A TW103109931 A TW 103109931A TW I636136 B TWI636136 B TW I636136B
- Authority
- TW
- Taiwan
- Prior art keywords
- temperature
- antibody
- yeast
- degrees
- promoter
- Prior art date
Links
- 238000000034 method Methods 0.000 title claims abstract description 143
- 240000004808 Saccharomyces cerevisiae Species 0.000 title claims abstract description 90
- 108090000765 processed proteins & peptides Proteins 0.000 title description 98
- 102000004196 processed proteins & peptides Human genes 0.000 title description 56
- 230000008859 change Effects 0.000 title description 21
- 108090000623 proteins and genes Proteins 0.000 claims abstract description 341
- 241000235058 Komagataella pastoris Species 0.000 claims abstract description 54
- 210000004027 cell Anatomy 0.000 claims description 112
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 claims description 74
- 210000005253 yeast cell Anatomy 0.000 claims description 72
- 230000002829 reductive effect Effects 0.000 claims description 55
- 238000004519 manufacturing process Methods 0.000 claims description 52
- 239000002609 medium Substances 0.000 claims description 48
- 241000235648 Pichia Species 0.000 claims description 42
- 238000004587 chromatography analysis Methods 0.000 claims description 34
- 239000001963 growth medium Substances 0.000 claims description 30
- 238000013518 transcription Methods 0.000 claims description 27
- 230000035897 transcription Effects 0.000 claims description 27
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims description 26
- 229910052799 carbon Inorganic materials 0.000 claims description 26
- 230000002159 abnormal effect Effects 0.000 claims description 23
- 238000012258 culturing Methods 0.000 claims description 21
- 208000035199 Tetraploidy Diseases 0.000 claims description 15
- 238000001502 gel electrophoresis Methods 0.000 claims description 15
- 230000001939 inductive effect Effects 0.000 claims description 14
- 241000320412 Ogataea angusta Species 0.000 claims description 12
- 101100327917 Caenorhabditis elegans chup-1 gene Proteins 0.000 claims description 10
- 101100502336 Komagataella pastoris FLD1 gene Proteins 0.000 claims description 10
- 241001452677 Ogataea methanolica Species 0.000 claims description 10
- 101100421128 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) SEI1 gene Proteins 0.000 claims description 10
- 101150069554 HIS4 gene Proteins 0.000 claims description 8
- 102000006602 glyceraldehyde-3-phosphate dehydrogenase Human genes 0.000 claims description 8
- 108020004445 glyceraldehyde-3-phosphate dehydrogenase Proteins 0.000 claims description 8
- FQVLRGLGWNWPSS-BXBUPLCLSA-N (4r,7s,10s,13s,16r)-16-acetamido-13-(1h-imidazol-5-ylmethyl)-10-methyl-6,9,12,15-tetraoxo-7-propan-2-yl-1,2-dithia-5,8,11,14-tetrazacycloheptadecane-4-carboxamide Chemical compound N1C(=O)[C@@H](NC(C)=O)CSSC[C@@H](C(N)=O)NC(=O)[C@H](C(C)C)NC(=O)[C@H](C)NC(=O)[C@@H]1CC1=CN=CN1 FQVLRGLGWNWPSS-BXBUPLCLSA-N 0.000 claims description 5
- 102100034035 Alcohol dehydrogenase 1A Human genes 0.000 claims description 5
- 102100039556 Galectin-4 Human genes 0.000 claims description 5
- 101000892220 Geobacillus thermodenitrificans (strain NG80-2) Long-chain-alcohol dehydrogenase 1 Proteins 0.000 claims description 5
- 241000238631 Hexapoda Species 0.000 claims description 5
- 101000780443 Homo sapiens Alcohol dehydrogenase 1A Proteins 0.000 claims description 5
- 101000608765 Homo sapiens Galectin-4 Proteins 0.000 claims description 5
- 101710122479 Isocitrate lyase 1 Proteins 0.000 claims description 5
- 241000270322 Lepidosauria Species 0.000 claims description 5
- 101150012394 PHO5 gene Proteins 0.000 claims description 5
- 101100191147 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) PHO3 gene Proteins 0.000 claims description 5
- 101150015622 pyk gene Proteins 0.000 claims description 5
- 230000003612 virological effect Effects 0.000 claims description 5
- 241000271566 Aves Species 0.000 claims description 4
- 230000001580 bacterial effect Effects 0.000 claims description 4
- 101150061302 och1 gene Proteins 0.000 claims description 4
- 102000007698 Alcohol dehydrogenase Human genes 0.000 claims description 3
- 108010021809 Alcohol dehydrogenase Proteins 0.000 claims description 3
- XUJNEKJLAYXESH-UHFFFAOYSA-N cysteine Natural products SCC(N)C(O)=O XUJNEKJLAYXESH-UHFFFAOYSA-N 0.000 claims description 3
- 235000018417 cysteine Nutrition 0.000 claims description 3
- 210000000805 cytoplasm Anatomy 0.000 claims description 3
- 230000003834 intracellular effect Effects 0.000 claims description 3
- 230000001568 sexual effect Effects 0.000 claims description 3
- MZOFCQQQCNRIBI-VMXHOPILSA-N (3s)-4-[[(2s)-1-[[(2s)-1-[[(1s)-1-carboxy-2-hydroxyethyl]amino]-4-methyl-1-oxopentan-2-yl]amino]-5-(diaminomethylideneamino)-1-oxopentan-2-yl]amino]-3-[[2-[[(2s)-2,6-diaminohexanoyl]amino]acetyl]amino]-4-oxobutanoic acid Chemical compound OC[C@@H](C(O)=O)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CCCN=C(N)N)NC(=O)[C@H](CC(O)=O)NC(=O)CNC(=O)[C@@H](N)CCCCN MZOFCQQQCNRIBI-VMXHOPILSA-N 0.000 claims description 2
- 108090000932 Calcitonin Gene-Related Peptide Proteins 0.000 claims description 2
- 102100025588 Calcitonin gene-related peptide 1 Human genes 0.000 claims description 2
- 102100021866 Hepatocyte growth factor Human genes 0.000 claims description 2
- 101000898034 Homo sapiens Hepatocyte growth factor Proteins 0.000 claims description 2
- 101001076408 Homo sapiens Interleukin-6 Proteins 0.000 claims description 2
- 101001098868 Homo sapiens Proprotein convertase subtilisin/kexin type 9 Proteins 0.000 claims description 2
- 101000868152 Homo sapiens Son of sevenless homolog 1 Proteins 0.000 claims description 2
- 108090001005 Interleukin-6 Proteins 0.000 claims description 2
- 102000004889 Interleukin-6 Human genes 0.000 claims description 2
- 102000015336 Nerve Growth Factor Human genes 0.000 claims description 2
- 108010025020 Nerve Growth Factor Proteins 0.000 claims description 2
- 102100038955 Proprotein convertase subtilisin/kexin type 9 Human genes 0.000 claims description 2
- 108060008682 Tumor Necrosis Factor Proteins 0.000 claims description 2
- 102000000852 Tumor Necrosis Factor-alpha Human genes 0.000 claims description 2
- 238000005029 sieve analysis Methods 0.000 claims description 2
- 102100036826 Aldehyde oxidase Human genes 0.000 claims 3
- 101000928314 Homo sapiens Aldehyde oxidase Proteins 0.000 claims 3
- LXJXRIRHZLFYRP-UHFFFAOYSA-N glyceraldehyde 3-phosphate Chemical compound O=CC(O)COP(O)(O)=O LXJXRIRHZLFYRP-UHFFFAOYSA-N 0.000 claims 2
- 241000235048 Meyerozyma guilliermondii Species 0.000 claims 1
- 239000004098 Tetracycline Substances 0.000 claims 1
- JZRWCGZRTZMZEH-UHFFFAOYSA-N Thiamine Natural products CC1=C(CCO)SC=[N+]1CC1=CN=C(C)N=C1N JZRWCGZRTZMZEH-UHFFFAOYSA-N 0.000 claims 1
- 229960002180 tetracycline Drugs 0.000 claims 1
- 229930101283 tetracycline Natural products 0.000 claims 1
- 235000019364 tetracycline Nutrition 0.000 claims 1
- 150000003522 tetracyclines Chemical class 0.000 claims 1
- KYMBYSLLVAOCFI-UHFFFAOYSA-N thiamine Chemical compound CC1=C(CCO)SCN1CC1=CN=C(C)N=C1N KYMBYSLLVAOCFI-UHFFFAOYSA-N 0.000 claims 1
- 229960003495 thiamine Drugs 0.000 claims 1
- 235000019157 thiamine Nutrition 0.000 claims 1
- 239000011721 thiamine Substances 0.000 claims 1
- 102000004169 proteins and genes Human genes 0.000 abstract description 195
- 102000054766 genetic haplotypes Human genes 0.000 abstract description 44
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 abstract description 9
- 230000001976 improved effect Effects 0.000 abstract description 5
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 206
- 235000018102 proteins Nutrition 0.000 description 188
- 238000000855 fermentation Methods 0.000 description 96
- 230000004151 fermentation Effects 0.000 description 96
- 235000014680 Saccharomyces cerevisiae Nutrition 0.000 description 84
- 229920001184 polypeptide Polymers 0.000 description 54
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 48
- 239000001301 oxygen Substances 0.000 description 48
- 229910052760 oxygen Inorganic materials 0.000 description 48
- 108020004414 DNA Proteins 0.000 description 43
- 230000001965 increasing effect Effects 0.000 description 42
- 239000000047 product Substances 0.000 description 41
- 210000003527 eukaryotic cell Anatomy 0.000 description 36
- LXJXRIRHZLFYRP-VKHMYHEASA-L (R)-2-Hydroxy-3-(phosphonooxy)-propanal Natural products O=C[C@H](O)COP([O-])([O-])=O LXJXRIRHZLFYRP-VKHMYHEASA-L 0.000 description 29
- LXJXRIRHZLFYRP-VKHMYHEASA-N D-glyceraldehyde 3-phosphate Chemical compound O=C[C@H](O)COP(O)(O)=O LXJXRIRHZLFYRP-VKHMYHEASA-N 0.000 description 29
- 230000000694 effects Effects 0.000 description 28
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 27
- 239000008103 glucose Substances 0.000 description 27
- 239000002054 inoculum Substances 0.000 description 27
- 239000002245 particle Substances 0.000 description 27
- 239000013598 vector Substances 0.000 description 27
- 235000011187 glycerol Nutrition 0.000 description 23
- 239000000523 sample Substances 0.000 description 23
- 241000894007 species Species 0.000 description 23
- 235000000346 sugar Nutrition 0.000 description 23
- 230000027455 binding Effects 0.000 description 22
- 239000012634 fragment Substances 0.000 description 22
- 230000014509 gene expression Effects 0.000 description 22
- 206010021143 Hypoxia Diseases 0.000 description 21
- 238000007792 addition Methods 0.000 description 20
- 230000012010 growth Effects 0.000 description 18
- 230000001146 hypoxic effect Effects 0.000 description 18
- 230000004060 metabolic process Effects 0.000 description 18
- 230000028327 secretion Effects 0.000 description 18
- 238000003756 stirring Methods 0.000 description 18
- 235000013882 gravy Nutrition 0.000 description 16
- 238000009825 accumulation Methods 0.000 description 15
- 239000000427 antigen Substances 0.000 description 15
- 102000036639 antigens Human genes 0.000 description 15
- 108091007433 antigens Proteins 0.000 description 15
- 241000283973 Oryctolagus cuniculus Species 0.000 description 14
- 239000000203 mixture Substances 0.000 description 14
- 108091033319 polynucleotide Proteins 0.000 description 14
- 102000040430 polynucleotide Human genes 0.000 description 14
- 239000002157 polynucleotide Substances 0.000 description 14
- 108091026890 Coding region Proteins 0.000 description 13
- 108060003951 Immunoglobulin Proteins 0.000 description 13
- 238000004458 analytical method Methods 0.000 description 13
- 102000018358 immunoglobulin Human genes 0.000 description 13
- 239000003550 marker Substances 0.000 description 13
- 230000001105 regulatory effect Effects 0.000 description 13
- 101100075829 Caenorhabditis elegans mab-3 gene Proteins 0.000 description 12
- 230000003247 decreasing effect Effects 0.000 description 12
- 230000016784 immunoglobulin production Effects 0.000 description 12
- 230000000241 respiratory effect Effects 0.000 description 12
- 239000006227 byproduct Substances 0.000 description 11
- 150000007523 nucleic acids Chemical class 0.000 description 11
- 239000000126 substance Substances 0.000 description 11
- 230000009466 transformation Effects 0.000 description 11
- YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 description 10
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 10
- 208000020584 Polyploidy Diseases 0.000 description 10
- 238000013019 agitation Methods 0.000 description 10
- 229940041514 candida albicans extract Drugs 0.000 description 10
- 239000013604 expression vector Substances 0.000 description 10
- 230000002068 genetic effect Effects 0.000 description 10
- 108020004999 messenger RNA Proteins 0.000 description 10
- 239000012138 yeast extract Substances 0.000 description 10
- 239000003814 drug Substances 0.000 description 9
- 102000039446 nucleic acids Human genes 0.000 description 9
- 108020004707 nucleic acids Proteins 0.000 description 9
- 235000015097 nutrients Nutrition 0.000 description 9
- 210000002706 plastid Anatomy 0.000 description 9
- 230000006798 recombination Effects 0.000 description 9
- 238000005215 recombination Methods 0.000 description 9
- 238000002415 sodium dodecyl sulfate polyacrylamide gel electrophoresis Methods 0.000 description 9
- -1 AOX1 Proteins 0.000 description 8
- 102000004190 Enzymes Human genes 0.000 description 8
- 108090000790 Enzymes Proteins 0.000 description 8
- 101150051118 PTM1 gene Proteins 0.000 description 8
- 102000007056 Recombinant Fusion Proteins Human genes 0.000 description 8
- 108010008281 Recombinant Fusion Proteins Proteins 0.000 description 8
- 235000001014 amino acid Nutrition 0.000 description 8
- 230000015572 biosynthetic process Effects 0.000 description 8
- 238000001514 detection method Methods 0.000 description 8
- 229940079593 drug Drugs 0.000 description 8
- 229940088598 enzyme Drugs 0.000 description 8
- 239000000499 gel Substances 0.000 description 8
- 238000003306 harvesting Methods 0.000 description 8
- 230000007246 mechanism Effects 0.000 description 8
- 238000012216 screening Methods 0.000 description 8
- 230000003248 secreting effect Effects 0.000 description 8
- 230000001225 therapeutic effect Effects 0.000 description 8
- 230000032258 transport Effects 0.000 description 8
- 101100476210 Caenorhabditis elegans rnt-1 gene Proteins 0.000 description 7
- 108010021625 Immunoglobulin Fragments Proteins 0.000 description 7
- 102000008394 Immunoglobulin Fragments Human genes 0.000 description 7
- 230000000295 complement effect Effects 0.000 description 7
- 238000011217 control strategy Methods 0.000 description 7
- 239000007789 gas Substances 0.000 description 7
- 238000000746 purification Methods 0.000 description 7
- 231100000419 toxicity Toxicity 0.000 description 7
- 230000001988 toxicity Effects 0.000 description 7
- 108020004705 Codon Proteins 0.000 description 6
- 102000008100 Human Serum Albumin Human genes 0.000 description 6
- 108091006905 Human Serum Albumin Proteins 0.000 description 6
- 125000003275 alpha amino acid group Chemical group 0.000 description 6
- 150000001413 amino acids Chemical class 0.000 description 6
- 230000008901 benefit Effects 0.000 description 6
- 230000002759 chromosomal effect Effects 0.000 description 6
- 239000003623 enhancer Substances 0.000 description 6
- 230000004927 fusion Effects 0.000 description 6
- 230000006872 improvement Effects 0.000 description 6
- 230000003993 interaction Effects 0.000 description 6
- 230000009467 reduction Effects 0.000 description 6
- FVAUCKIRQBBSSJ-UHFFFAOYSA-M sodium iodide Chemical compound [Na+].[I-] FVAUCKIRQBBSSJ-UHFFFAOYSA-M 0.000 description 6
- 229910021654 trace metal Inorganic materials 0.000 description 6
- 230000014616 translation Effects 0.000 description 6
- 108020004684 Internal Ribosome Entry Sites Proteins 0.000 description 5
- 102000006010 Protein Disulfide-Isomerase Human genes 0.000 description 5
- 241000831652 Salinivibrio sharmensis Species 0.000 description 5
- 229960002685 biotin Drugs 0.000 description 5
- 235000020958 biotin Nutrition 0.000 description 5
- 239000011616 biotin Substances 0.000 description 5
- 239000001569 carbon dioxide Substances 0.000 description 5
- 229910002092 carbon dioxide Inorganic materials 0.000 description 5
- 210000001840 diploid cell Anatomy 0.000 description 5
- 230000006870 function Effects 0.000 description 5
- 229940072221 immunoglobulins Drugs 0.000 description 5
- 238000005259 measurement Methods 0.000 description 5
- 108020003519 protein disulfide isomerase Proteins 0.000 description 5
- 238000013519 translation Methods 0.000 description 5
- 108091032973 (ribonucleotides)n+m Proteins 0.000 description 4
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 4
- 241000222120 Candida <Saccharomycetales> Species 0.000 description 4
- SRBFZHDQGSBBOR-IOVATXLUSA-N D-xylopyranose Chemical compound O[C@@H]1COC(O)[C@H](O)[C@H]1O SRBFZHDQGSBBOR-IOVATXLUSA-N 0.000 description 4
- 241000196324 Embryophyta Species 0.000 description 4
- 108090001090 Lectins Proteins 0.000 description 4
- 102000004856 Lectins Human genes 0.000 description 4
- 108091028043 Nucleic acid sequence Proteins 0.000 description 4
- 241000700159 Rattus Species 0.000 description 4
- QAOWNCQODCNURD-UHFFFAOYSA-N Sulfuric acid Chemical compound OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 description 4
- 101710120037 Toxin CcdB Proteins 0.000 description 4
- 241000700605 Viruses Species 0.000 description 4
- 230000003321 amplification Effects 0.000 description 4
- PYMYPHUHKUWMLA-UHFFFAOYSA-N arabinose Natural products OCC(O)C(O)C(O)C=O PYMYPHUHKUWMLA-UHFFFAOYSA-N 0.000 description 4
- 230000009286 beneficial effect Effects 0.000 description 4
- SRBFZHDQGSBBOR-UHFFFAOYSA-N beta-D-Pyranose-Lyxose Natural products OC1COC(O)C(O)C1O SRBFZHDQGSBBOR-UHFFFAOYSA-N 0.000 description 4
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 4
- 239000003795 chemical substances by application Substances 0.000 description 4
- 210000000349 chromosome Anatomy 0.000 description 4
- 239000002299 complementary DNA Substances 0.000 description 4
- 230000007423 decrease Effects 0.000 description 4
- 239000012527 feed solution Substances 0.000 description 4
- 238000004128 high performance liquid chromatography Methods 0.000 description 4
- 238000011081 inoculation Methods 0.000 description 4
- 239000002523 lectin Substances 0.000 description 4
- WRUGWIBCXHJTDG-UHFFFAOYSA-L magnesium sulfate heptahydrate Chemical compound O.O.O.O.O.O.O.[Mg+2].[O-]S([O-])(=O)=O WRUGWIBCXHJTDG-UHFFFAOYSA-L 0.000 description 4
- 229940061634 magnesium sulfate heptahydrate Drugs 0.000 description 4
- 238000004949 mass spectrometry Methods 0.000 description 4
- 230000002503 metabolic effect Effects 0.000 description 4
- 229910000402 monopotassium phosphate Inorganic materials 0.000 description 4
- 235000019796 monopotassium phosphate Nutrition 0.000 description 4
- 230000035772 mutation Effects 0.000 description 4
- 238000003199 nucleic acid amplification method Methods 0.000 description 4
- 238000005457 optimization Methods 0.000 description 4
- PJNZPQUBCPKICU-UHFFFAOYSA-N phosphoric acid;potassium Chemical compound [K].OP(O)(O)=O PJNZPQUBCPKICU-UHFFFAOYSA-N 0.000 description 4
- 238000002360 preparation method Methods 0.000 description 4
- 230000008569 process Effects 0.000 description 4
- 238000011160 research Methods 0.000 description 4
- 238000003998 size exclusion chromatography high performance liquid chromatography Methods 0.000 description 4
- NLJMYIDDQXHKNR-UHFFFAOYSA-K sodium citrate Chemical compound O.O.[Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O NLJMYIDDQXHKNR-UHFFFAOYSA-K 0.000 description 4
- 229960000999 sodium citrate dihydrate Drugs 0.000 description 4
- 239000000243 solution Substances 0.000 description 4
- 238000006467 substitution reaction Methods 0.000 description 4
- 238000003786 synthesis reaction Methods 0.000 description 4
- 238000012360 testing method Methods 0.000 description 4
- JIAARYAFYJHUJI-UHFFFAOYSA-L zinc dichloride Chemical compound [Cl-].[Cl-].[Zn+2] JIAARYAFYJHUJI-UHFFFAOYSA-L 0.000 description 4
- OWEGMIWEEQEYGQ-UHFFFAOYSA-N 100676-05-9 Natural products OC1C(O)C(O)C(CO)OC1OCC1C(O)C(O)C(O)C(OC2C(OC(O)C(O)C2O)CO)O1 OWEGMIWEEQEYGQ-UHFFFAOYSA-N 0.000 description 3
- 241000894006 Bacteria Species 0.000 description 3
- 241000283690 Bos taurus Species 0.000 description 3
- 241000251730 Chondrichthyes Species 0.000 description 3
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 description 3
- 102000004163 DNA-directed RNA polymerases Human genes 0.000 description 3
- 108090000626 DNA-directed RNA polymerases Proteins 0.000 description 3
- BWGNESOTFCXPMA-UHFFFAOYSA-N Dihydrogen disulfide Chemical compound SS BWGNESOTFCXPMA-UHFFFAOYSA-N 0.000 description 3
- 229930091371 Fructose Natural products 0.000 description 3
- 239000005715 Fructose Substances 0.000 description 3
- RFSUNEUAIZKAJO-ARQDHWQXSA-N Fructose Chemical compound OC[C@H]1O[C@](O)(CO)[C@@H](O)[C@@H]1O RFSUNEUAIZKAJO-ARQDHWQXSA-N 0.000 description 3
- 241000282412 Homo Species 0.000 description 3
- XUJNEKJLAYXESH-REOHCLBHSA-N L-Cysteine Chemical compound SC[C@H](N)C(O)=O XUJNEKJLAYXESH-REOHCLBHSA-N 0.000 description 3
- GUBGYTABKSRVRQ-PICCSMPSSA-N Maltose Natural products O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@@H](CO)OC(O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-PICCSMPSSA-N 0.000 description 3
- 241001465754 Metazoa Species 0.000 description 3
- 108010014251 Muramidase Proteins 0.000 description 3
- 102000016943 Muramidase Human genes 0.000 description 3
- 108010062010 N-Acetylmuramoyl-L-alanine Amidase Proteins 0.000 description 3
- 241001494479 Pecora Species 0.000 description 3
- 241000235070 Saccharomyces Species 0.000 description 3
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 3
- 210000004507 artificial chromosome Anatomy 0.000 description 3
- GUBGYTABKSRVRQ-QUYVBRFLSA-N beta-maltose Chemical compound OC[C@H]1O[C@H](O[C@H]2[C@H](O)[C@@H](O)[C@H](O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@@H]1O GUBGYTABKSRVRQ-QUYVBRFLSA-N 0.000 description 3
- 230000003115 biocidal effect Effects 0.000 description 3
- 230000004071 biological effect Effects 0.000 description 3
- 230000001851 biosynthetic effect Effects 0.000 description 3
- GUTLYIVDDKVIGB-UHFFFAOYSA-N cobalt atom Chemical compound [Co] GUTLYIVDDKVIGB-UHFFFAOYSA-N 0.000 description 3
- 238000007796 conventional method Methods 0.000 description 3
- 229910052802 copper Inorganic materials 0.000 description 3
- 239000010949 copper Substances 0.000 description 3
- 238000012217 deletion Methods 0.000 description 3
- 230000037430 deletion Effects 0.000 description 3
- 230000009977 dual effect Effects 0.000 description 3
- 239000012636 effector Substances 0.000 description 3
- 230000002538 fungal effect Effects 0.000 description 3
- 210000003783 haploid cell Anatomy 0.000 description 3
- 230000036541 health Effects 0.000 description 3
- 230000007954 hypoxia Effects 0.000 description 3
- 230000001900 immune effect Effects 0.000 description 3
- 238000000338 in vitro Methods 0.000 description 3
- 238000011534 incubation Methods 0.000 description 3
- 239000004325 lysozyme Substances 0.000 description 3
- 229960000274 lysozyme Drugs 0.000 description 3
- 235000010335 lysozyme Nutrition 0.000 description 3
- 239000000463 material Substances 0.000 description 3
- 230000000813 microbial effect Effects 0.000 description 3
- 230000004048 modification Effects 0.000 description 3
- 238000012986 modification Methods 0.000 description 3
- YBYRMVIVWMBXKQ-UHFFFAOYSA-N phenylmethanesulfonyl fluoride Chemical compound FS(=O)(=O)CC1=CC=CC=C1 YBYRMVIVWMBXKQ-UHFFFAOYSA-N 0.000 description 3
- 239000012925 reference material Substances 0.000 description 3
- 230000010076 replication Effects 0.000 description 3
- 230000000153 supplemental effect Effects 0.000 description 3
- 239000003053 toxin Substances 0.000 description 3
- 231100000765 toxin Toxicity 0.000 description 3
- 108700012359 toxins Proteins 0.000 description 3
- 230000005030 transcription termination Effects 0.000 description 3
- 238000012546 transfer Methods 0.000 description 3
- 230000001131 transforming effect Effects 0.000 description 3
- 210000003462 vein Anatomy 0.000 description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 3
- HDTRYLNUVZCQOY-UHFFFAOYSA-N α-D-glucopyranosyl-α-D-glucopyranoside Natural products OC1C(O)C(O)C(CO)OC1OC1C(O)C(O)C(O)C(CO)O1 HDTRYLNUVZCQOY-UHFFFAOYSA-N 0.000 description 2
- 101710194180 Alcohol oxidase 1 Proteins 0.000 description 2
- GUBGYTABKSRVRQ-XLOQQCSPSA-N Alpha-Lactose Chemical compound O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@H]1O[C@@H]1[C@@H](CO)O[C@H](O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-XLOQQCSPSA-N 0.000 description 2
- VHUUQVKOLVNVRT-UHFFFAOYSA-N Ammonium hydroxide Chemical compound [NH4+].[OH-] VHUUQVKOLVNVRT-UHFFFAOYSA-N 0.000 description 2
- 108010083359 Antigen Receptors Proteins 0.000 description 2
- 241000283707 Capra Species 0.000 description 2
- 239000004215 Carbon black (E152) Substances 0.000 description 2
- ZAMOUSCENKQFHK-UHFFFAOYSA-N Chlorine atom Chemical compound [Cl] ZAMOUSCENKQFHK-UHFFFAOYSA-N 0.000 description 2
- KRKNYBCHXYNGOX-UHFFFAOYSA-K Citrate Chemical compound [O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O KRKNYBCHXYNGOX-UHFFFAOYSA-K 0.000 description 2
- 102000001493 Cyclophilins Human genes 0.000 description 2
- 108010068682 Cyclophilins Proteins 0.000 description 2
- FBPFZTCFMRRESA-FSIIMWSLSA-N D-Glucitol Natural products OC[C@H](O)[C@H](O)[C@@H](O)[C@H](O)CO FBPFZTCFMRRESA-FSIIMWSLSA-N 0.000 description 2
- FBPFZTCFMRRESA-KVTDHHQDSA-N D-Mannitol Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)[C@H](O)CO FBPFZTCFMRRESA-KVTDHHQDSA-N 0.000 description 2
- FBPFZTCFMRRESA-JGWLITMVSA-N D-glucitol Chemical compound OC[C@H](O)[C@@H](O)[C@H](O)[C@H](O)CO FBPFZTCFMRRESA-JGWLITMVSA-N 0.000 description 2
- WQZGKKKJIJFFOK-QTVWNMPRSA-N D-mannopyranose Chemical compound OC[C@H]1OC(O)[C@@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-QTVWNMPRSA-N 0.000 description 2
- HMFHBZSHGGEWLO-SOOFDHNKSA-N D-ribofuranose Chemical compound OC[C@H]1OC(O)[C@H](O)[C@@H]1O HMFHBZSHGGEWLO-SOOFDHNKSA-N 0.000 description 2
- 101710088194 Dehydrogenase Proteins 0.000 description 2
- 241000287828 Gallus gallus Species 0.000 description 2
- 108010054477 Immunoglobulin Fab Fragments Proteins 0.000 description 2
- 102000001706 Immunoglobulin Fab Fragments Human genes 0.000 description 2
- 241000235649 Kluyveromyces Species 0.000 description 2
- GUBGYTABKSRVRQ-QKKXKWKRSA-N Lactose Natural products OC[C@H]1O[C@@H](O[C@H]2[C@H](O)[C@@H](O)C(O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@H]1O GUBGYTABKSRVRQ-QKKXKWKRSA-N 0.000 description 2
- 241000124008 Mammalia Species 0.000 description 2
- 229930195725 Mannitol Natural products 0.000 description 2
- 241001221837 Ogataea thermomethanolica Species 0.000 description 2
- 241000235652 Pachysolen Species 0.000 description 2
- MUPFEKGTMRGPLJ-RMMQSMQOSA-N Raffinose Natural products O(C[C@H]1[C@@H](O)[C@H](O)[C@@H](O)[C@@H](O[C@@]2(CO)[C@H](O)[C@@H](O)[C@@H](CO)O2)O1)[C@@H]1[C@H](O)[C@@H](O)[C@@H](O)[C@@H](CO)O1 MUPFEKGTMRGPLJ-RMMQSMQOSA-N 0.000 description 2
- 102000004389 Ribonucleoproteins Human genes 0.000 description 2
- 108010081734 Ribonucleoproteins Proteins 0.000 description 2
- PYMYPHUHKUWMLA-LMVFSUKVSA-N Ribose Natural products OC[C@@H](O)[C@@H](O)[C@@H](O)C=O PYMYPHUHKUWMLA-LMVFSUKVSA-N 0.000 description 2
- 241000283984 Rodentia Species 0.000 description 2
- 108010003723 Single-Domain Antibodies Proteins 0.000 description 2
- 238000002105 Southern blotting Methods 0.000 description 2
- HDTRYLNUVZCQOY-WSWWMNSNSA-N Trehalose Natural products O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-WSWWMNSNSA-N 0.000 description 2
- MUPFEKGTMRGPLJ-UHFFFAOYSA-N UNPD196149 Natural products OC1C(O)C(CO)OC1(CO)OC1C(O)C(O)C(O)C(COC2C(C(O)C(O)C(CO)O2)O)O1 MUPFEKGTMRGPLJ-UHFFFAOYSA-N 0.000 description 2
- 241000235013 Yarrowia Species 0.000 description 2
- 238000002835 absorbance Methods 0.000 description 2
- 230000004913 activation Effects 0.000 description 2
- 230000002411 adverse Effects 0.000 description 2
- GZCGUPFRVQAUEE-SLPGGIOYSA-N aldehydo-D-glucose Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)[C@@H](O)C=O GZCGUPFRVQAUEE-SLPGGIOYSA-N 0.000 description 2
- HDTRYLNUVZCQOY-LIZSDCNHSA-N alpha,alpha-trehalose Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-LIZSDCNHSA-N 0.000 description 2
- HMFHBZSHGGEWLO-UHFFFAOYSA-N alpha-D-Furanose-Ribose Natural products OCC1OC(O)C(O)C1O HMFHBZSHGGEWLO-UHFFFAOYSA-N 0.000 description 2
- WQZGKKKJIJFFOK-PHYPRBDBSA-N alpha-D-galactose Chemical compound OC[C@H]1O[C@H](O)[C@H](O)[C@@H](O)[C@H]1O WQZGKKKJIJFFOK-PHYPRBDBSA-N 0.000 description 2
- 125000000539 amino acid group Chemical group 0.000 description 2
- LFVGISIMTYGQHF-UHFFFAOYSA-N ammonium dihydrogen phosphate Chemical compound [NH4+].OP(O)([O-])=O LFVGISIMTYGQHF-UHFFFAOYSA-N 0.000 description 2
- 229910000387 ammonium dihydrogen phosphate Inorganic materials 0.000 description 2
- 239000000908 ammonium hydroxide Substances 0.000 description 2
- 230000003698 anagen phase Effects 0.000 description 2
- 229940089206 anhydrous dextrose Drugs 0.000 description 2
- 239000003242 anti bacterial agent Substances 0.000 description 2
- 210000000628 antibody-producing cell Anatomy 0.000 description 2
- 239000002518 antifoaming agent Substances 0.000 description 2
- 238000013459 approach Methods 0.000 description 2
- PYMYPHUHKUWMLA-WDCZJNDASA-N arabinose Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)C=O PYMYPHUHKUWMLA-WDCZJNDASA-N 0.000 description 2
- 210000003719 b-lymphocyte Anatomy 0.000 description 2
- 238000012365 batch cultivation Methods 0.000 description 2
- 108010051210 beta-Fructofuranosidase Proteins 0.000 description 2
- DLRVVLDZNNYCBX-ZZFZYMBESA-N beta-melibiose Chemical compound O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@@H]1OC[C@@H]1[C@@H](O)[C@H](O)[C@@H](O)[C@H](O)O1 DLRVVLDZNNYCBX-ZZFZYMBESA-N 0.000 description 2
- 229960000074 biopharmaceutical Drugs 0.000 description 2
- KGBXLFKZBHKPEV-UHFFFAOYSA-N boric acid Chemical compound OB(O)O KGBXLFKZBHKPEV-UHFFFAOYSA-N 0.000 description 2
- 239000004327 boric acid Substances 0.000 description 2
- 238000005251 capillar electrophoresis Methods 0.000 description 2
- 235000013330 chicken meat Nutrition 0.000 description 2
- 239000000460 chlorine Substances 0.000 description 2
- 229910052801 chlorine Inorganic materials 0.000 description 2
- 238000003776 cleavage reaction Methods 0.000 description 2
- 239000010941 cobalt Substances 0.000 description 2
- 229910017052 cobalt Inorganic materials 0.000 description 2
- JZCCFEFSEZPSOG-UHFFFAOYSA-L copper(II) sulfate pentahydrate Chemical compound O.O.O.O.O.[Cu+2].[O-]S([O-])(=O)=O JZCCFEFSEZPSOG-UHFFFAOYSA-L 0.000 description 2
- 210000004748 cultured cell Anatomy 0.000 description 2
- 125000000151 cysteine group Chemical group N[C@@H](CS)C(=O)* 0.000 description 2
- 239000003599 detergent Substances 0.000 description 2
- 238000010828 elution Methods 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 238000001943 fluorescence-activated cell sorting Methods 0.000 description 2
- 238000007499 fusion processing Methods 0.000 description 2
- 229930182830 galactose Natural products 0.000 description 2
- 238000010353 genetic engineering Methods 0.000 description 2
- 230000036252 glycation Effects 0.000 description 2
- 229930195733 hydrocarbon Natural products 0.000 description 2
- 150000002430 hydrocarbons Chemical class 0.000 description 2
- 230000002163 immunogen Effects 0.000 description 2
- 230000005847 immunogenicity Effects 0.000 description 2
- 239000012535 impurity Substances 0.000 description 2
- 230000002401 inhibitory effect Effects 0.000 description 2
- 230000000977 initiatory effect Effects 0.000 description 2
- 238000002347 injection Methods 0.000 description 2
- 239000007924 injection Substances 0.000 description 2
- 239000001573 invertase Substances 0.000 description 2
- 235000011073 invertase Nutrition 0.000 description 2
- 238000004255 ion exchange chromatography Methods 0.000 description 2
- SURQXAFEQWPFPV-UHFFFAOYSA-L iron(2+) sulfate heptahydrate Chemical compound O.O.O.O.O.O.O.[Fe+2].[O-]S([O-])(=O)=O SURQXAFEQWPFPV-UHFFFAOYSA-L 0.000 description 2
- 238000001155 isoelectric focusing Methods 0.000 description 2
- 229930027917 kanamycin Natural products 0.000 description 2
- 229960000318 kanamycin Drugs 0.000 description 2
- SBUJHOSQTJFQJX-NOAMYHISSA-N kanamycin Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CN)O[C@@H]1O[C@H]1[C@H](O)[C@@H](O[C@@H]2[C@@H]([C@@H](N)[C@H](O)[C@@H](CO)O2)O)[C@H](N)C[C@@H]1N SBUJHOSQTJFQJX-NOAMYHISSA-N 0.000 description 2
- 229930182823 kanamycin A Natural products 0.000 description 2
- 239000008101 lactose Substances 0.000 description 2
- 230000000670 limiting effect Effects 0.000 description 2
- ISPYRSDWRDQNSW-UHFFFAOYSA-L manganese(II) sulfate monohydrate Chemical compound O.[Mn+2].[O-]S([O-])(=O)=O ISPYRSDWRDQNSW-UHFFFAOYSA-L 0.000 description 2
- 239000000594 mannitol Substances 0.000 description 2
- 235000010355 mannitol Nutrition 0.000 description 2
- 230000013011 mating Effects 0.000 description 2
- 239000011159 matrix material Substances 0.000 description 2
- 235000013372 meat Nutrition 0.000 description 2
- 239000012528 membrane Substances 0.000 description 2
- 235000019837 monoammonium phosphate Nutrition 0.000 description 2
- 238000002703 mutagenesis Methods 0.000 description 2
- 231100000350 mutagenesis Toxicity 0.000 description 2
- 229910052757 nitrogen Inorganic materials 0.000 description 2
- 238000011017 operating method Methods 0.000 description 2
- 230000003647 oxidation Effects 0.000 description 2
- 238000007254 oxidation reaction Methods 0.000 description 2
- 238000006213 oxygenation reaction Methods 0.000 description 2
- 230000005298 paramagnetic effect Effects 0.000 description 2
- 230000001323 posttranslational effect Effects 0.000 description 2
- OTYBMLCTZGSZBG-UHFFFAOYSA-L potassium sulfate Chemical compound [K+].[K+].[O-]S([O-])(=O)=O OTYBMLCTZGSZBG-UHFFFAOYSA-L 0.000 description 2
- 229910052939 potassium sulfate Inorganic materials 0.000 description 2
- 235000011151 potassium sulphates Nutrition 0.000 description 2
- 238000004886 process control Methods 0.000 description 2
- 238000012545 processing Methods 0.000 description 2
- 230000002062 proliferating effect Effects 0.000 description 2
- 230000017854 proteolysis Effects 0.000 description 2
- 230000002797 proteolythic effect Effects 0.000 description 2
- MUPFEKGTMRGPLJ-ZQSKZDJDSA-N raffinose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO[C@@H]2[C@@H]([C@@H](O)[C@@H](O)[C@@H](CO)O2)O)O1 MUPFEKGTMRGPLJ-ZQSKZDJDSA-N 0.000 description 2
- 238000010188 recombinant method Methods 0.000 description 2
- 230000004044 response Effects 0.000 description 2
- 108091008146 restriction endonucleases Proteins 0.000 description 2
- 230000000717 retained effect Effects 0.000 description 2
- 238000012552 review Methods 0.000 description 2
- 238000005070 sampling Methods 0.000 description 2
- 230000007017 scission Effects 0.000 description 2
- 239000011780 sodium chloride Substances 0.000 description 2
- 235000009518 sodium iodide Nutrition 0.000 description 2
- RWVGQQGBQSJDQV-UHFFFAOYSA-M sodium;3-[[4-[(e)-[4-(4-ethoxyanilino)phenyl]-[4-[ethyl-[(3-sulfonatophenyl)methyl]azaniumylidene]-2-methylcyclohexa-2,5-dien-1-ylidene]methyl]-n-ethyl-3-methylanilino]methyl]benzenesulfonate Chemical compound [Na+].C1=CC(OCC)=CC=C1NC1=CC=C(C(=C2C(=CC(C=C2)=[N+](CC)CC=2C=C(C=CC=2)S([O-])(=O)=O)C)C=2C(=CC(=CC=2)N(CC)CC=2C=C(C=CC=2)S([O-])(=O)=O)C)C=C1 RWVGQQGBQSJDQV-UHFFFAOYSA-M 0.000 description 2
- 239000000600 sorbitol Substances 0.000 description 2
- 230000028070 sporulation Effects 0.000 description 2
- 239000011573 trace mineral Substances 0.000 description 2
- 235000013619 trace mineral Nutrition 0.000 description 2
- 239000003981 vehicle Substances 0.000 description 2
- 238000001262 western blot Methods 0.000 description 2
- 239000011592 zinc chloride Substances 0.000 description 2
- 235000005074 zinc chloride Nutrition 0.000 description 2
- OWFJMIVZYSDULZ-PXOLEDIWSA-N (4s,4ar,5s,5ar,6s,12ar)-4-(dimethylamino)-1,5,6,10,11,12a-hexahydroxy-6-methyl-3,12-dioxo-4,4a,5,5a-tetrahydrotetracene-2-carboxamide Chemical compound C1=CC=C2[C@](O)(C)[C@H]3[C@H](O)[C@H]4[C@H](N(C)C)C(=O)C(C(N)=O)=C(O)[C@@]4(O)C(=O)C3=C(O)C2=C1O OWFJMIVZYSDULZ-PXOLEDIWSA-N 0.000 description 1
- DBTMGCOVALSLOR-UHFFFAOYSA-N 32-alpha-galactosyl-3-alpha-galactosyl-galactose Natural products OC1C(O)C(O)C(CO)OC1OC1C(O)C(OC2C(C(CO)OC(O)C2O)O)OC(CO)C1O DBTMGCOVALSLOR-UHFFFAOYSA-N 0.000 description 1
- QFVHZQCOUORWEI-UHFFFAOYSA-N 4-[(4-anilino-5-sulfonaphthalen-1-yl)diazenyl]-5-hydroxynaphthalene-2,7-disulfonic acid Chemical compound C=12C(O)=CC(S(O)(=O)=O)=CC2=CC(S(O)(=O)=O)=CC=1N=NC(C1=CC=CC(=C11)S(O)(=O)=O)=CC=C1NC1=CC=CC=C1 QFVHZQCOUORWEI-UHFFFAOYSA-N 0.000 description 1
- 101150076082 ALD5 gene Proteins 0.000 description 1
- 101150030091 AQR1 gene Proteins 0.000 description 1
- QTBSBXVTEAMEQO-UHFFFAOYSA-M Acetate Chemical compound CC([O-])=O QTBSBXVTEAMEQO-UHFFFAOYSA-M 0.000 description 1
- 108010052946 Activin Receptors Proteins 0.000 description 1
- 102000018918 Activin Receptors Human genes 0.000 description 1
- 108010059616 Activins Proteins 0.000 description 1
- 102000005606 Activins Human genes 0.000 description 1
- 108010025188 Alcohol oxidase Proteins 0.000 description 1
- 102100038910 Alpha-enolase Human genes 0.000 description 1
- 102100022992 Anoctamin-1 Human genes 0.000 description 1
- 102000006306 Antigen Receptors Human genes 0.000 description 1
- 101100389688 Arabidopsis thaliana AERO1 gene Proteins 0.000 description 1
- 101100170365 Arabidopsis thaliana At5g60805 gene Proteins 0.000 description 1
- 101100353069 Arabidopsis thaliana PPOX1 gene Proteins 0.000 description 1
- 241001508785 Arxiozyma Species 0.000 description 1
- 241000228245 Aspergillus niger Species 0.000 description 1
- 108090001008 Avidin Proteins 0.000 description 1
- 101150051010 BNA1 gene Proteins 0.000 description 1
- 101100434663 Bacillus subtilis (strain 168) fbaA gene Proteins 0.000 description 1
- 241000221198 Basidiomycota Species 0.000 description 1
- 239000002028 Biomass Substances 0.000 description 1
- 102000015081 Blood Coagulation Factors Human genes 0.000 description 1
- 108010039209 Blood Coagulation Factors Proteins 0.000 description 1
- 241000255789 Bombyx mori Species 0.000 description 1
- 101100297249 Borrelia burgdorferi (strain ATCC 35210 / B31 / CIP 102532 / DSM 4680) pfp gene Proteins 0.000 description 1
- 241000178289 Botryozyma Species 0.000 description 1
- 241000722885 Brettanomyces Species 0.000 description 1
- 241000235172 Bullera Species 0.000 description 1
- 101150067142 CYS3 gene Proteins 0.000 description 1
- 101150006642 CYS4 gene Proteins 0.000 description 1
- 101100455752 Caenorhabditis elegans lys-3 gene Proteins 0.000 description 1
- 101100480861 Caldanaerobacter subterraneus subsp. tengcongensis (strain DSM 15242 / JCM 11007 / NBRC 100824 / MB4) tdh gene Proteins 0.000 description 1
- 101100208421 Candida albicans (strain SC5314 / ATCC MYA-2876) TMP1 gene Proteins 0.000 description 1
- 101100445809 Candida albicans (strain SC5314 / ATCC MYA-2876) XOG1 gene Proteins 0.000 description 1
- 101100447466 Candida albicans (strain WO-1) TDH1 gene Proteins 0.000 description 1
- 241000282472 Canis lupus familiaris Species 0.000 description 1
- 102100025597 Caspase-4 Human genes 0.000 description 1
- 108050001186 Chaperonin Cpn60 Proteins 0.000 description 1
- 102000052603 Chaperonins Human genes 0.000 description 1
- 101100064921 Chlamydomonas reinhardtii EFTS gene Proteins 0.000 description 1
- 108091062157 Cis-regulatory element Proteins 0.000 description 1
- 241001508787 Citeromyces Species 0.000 description 1
- 108700010070 Codon Usage Proteins 0.000 description 1
- 108010047041 Complementarity Determining Regions Proteins 0.000 description 1
- 102100029079 Cytochrome c oxidase assembly protein COX15 homolog Human genes 0.000 description 1
- 102000004127 Cytokines Human genes 0.000 description 1
- 108090000695 Cytokines Proteins 0.000 description 1
- MNQZXJOMYWMBOU-VKHMYHEASA-N D-glyceraldehyde Chemical compound OC[C@@H](O)C=O MNQZXJOMYWMBOU-VKHMYHEASA-N 0.000 description 1
- RXVWSYJTUUKTEA-UHFFFAOYSA-N D-maltotriose Natural products OC1C(O)C(OC(C(O)CO)C(O)C(O)C=O)OC(CO)C1OC1C(O)C(O)C(O)C(CO)O1 RXVWSYJTUUKTEA-UHFFFAOYSA-N 0.000 description 1
- 101150010082 DGK1 gene Proteins 0.000 description 1
- 102000053602 DNA Human genes 0.000 description 1
- 238000012270 DNA recombination Methods 0.000 description 1
- 102100030960 DNA replication licensing factor MCM2 Human genes 0.000 description 1
- 241000235035 Debaryomyces Species 0.000 description 1
- MYMOFIZGZYHOMD-UHFFFAOYSA-N Dioxygen Chemical compound O=O MYMOFIZGZYHOMD-UHFFFAOYSA-N 0.000 description 1
- 101100063288 Drosophila melanogaster Dgk gene Proteins 0.000 description 1
- 101150050596 EFT2 gene Proteins 0.000 description 1
- 101150106008 ERG11 gene Proteins 0.000 description 1
- 101150047030 ERO1 gene Proteins 0.000 description 1
- 101150114024 ERV14 gene Proteins 0.000 description 1
- 101150000833 EXG1 gene Proteins 0.000 description 1
- 108700041152 Endoplasmic Reticulum Chaperone BiP Proteins 0.000 description 1
- 102100021451 Endoplasmic reticulum chaperone BiP Human genes 0.000 description 1
- 241001465321 Eremothecium Species 0.000 description 1
- 102100035177 Ergosterol biosynthetic protein 28 homolog Human genes 0.000 description 1
- 241000588724 Escherichia coli Species 0.000 description 1
- 241000701959 Escherichia virus Lambda Species 0.000 description 1
- 101150095274 FBA1 gene Proteins 0.000 description 1
- 101150108435 GLK1 gene Proteins 0.000 description 1
- 101150081655 GPM1 gene Proteins 0.000 description 1
- 108700028146 Genetic Enhancer Elements Proteins 0.000 description 1
- 108010073178 Glucan 1,4-alpha-Glucosidase Proteins 0.000 description 1
- 102100022087 Granzyme M Human genes 0.000 description 1
- 101150106451 HEM13 gene Proteins 0.000 description 1
- 101150045879 HEM14 gene Proteins 0.000 description 1
- 101100246753 Halobacterium salinarum (strain ATCC 700922 / JCM 11081 / NRC-1) pyrF gene Proteins 0.000 description 1
- 108010004889 Heat-Shock Proteins Proteins 0.000 description 1
- 102000002812 Heat-Shock Proteins Human genes 0.000 description 1
- 108010019372 Heterogeneous-Nuclear Ribonucleoproteins Proteins 0.000 description 1
- 102000006479 Heterogeneous-Nuclear Ribonucleoproteins Human genes 0.000 description 1
- 101000882335 Homo sapiens Alpha-enolase Proteins 0.000 description 1
- 101000757261 Homo sapiens Anoctamin-1 Proteins 0.000 description 1
- 101000897480 Homo sapiens C-C motif chemokine 2 Proteins 0.000 description 1
- 101000855412 Homo sapiens Carbamoyl-phosphate synthase [ammonia], mitochondrial Proteins 0.000 description 1
- 101000933112 Homo sapiens Caspase-4 Proteins 0.000 description 1
- 101000770637 Homo sapiens Cytochrome c oxidase assembly protein COX15 homolog Proteins 0.000 description 1
- 101000583807 Homo sapiens DNA replication licensing factor MCM2 Proteins 0.000 description 1
- 101000876557 Homo sapiens Ergosterol biosynthetic protein 28 homolog Proteins 0.000 description 1
- 101000900697 Homo sapiens Granzyme M Proteins 0.000 description 1
- 101000998969 Homo sapiens Inositol-3-phosphate synthase 1 Proteins 0.000 description 1
- 101100182720 Homo sapiens LY6E gene Proteins 0.000 description 1
- 101001018100 Homo sapiens Lysozyme C Proteins 0.000 description 1
- 101000630572 Homo sapiens Molybdopterin-synthase sulfurtransferase Proteins 0.000 description 1
- 101001128284 Homo sapiens N-alpha-acetyltransferase 30 Proteins 0.000 description 1
- 101000636582 Homo sapiens N-alpha-acetyltransferase 50 Proteins 0.000 description 1
- 101000983292 Homo sapiens N-fatty-acyl-amino acid synthase/hydrolase PM20D1 Proteins 0.000 description 1
- 101001123331 Homo sapiens Peroxisome proliferator-activated receptor gamma coactivator 1-alpha Proteins 0.000 description 1
- 101000579123 Homo sapiens Phosphoglycerate kinase 1 Proteins 0.000 description 1
- 101001047090 Homo sapiens Potassium voltage-gated channel subfamily H member 2 Proteins 0.000 description 1
- 101000982628 Homo sapiens Prolyl 3-hydroxylase OGFOD1 Proteins 0.000 description 1
- 101000716803 Homo sapiens Protein SCO1 homolog, mitochondrial Proteins 0.000 description 1
- 101000908580 Homo sapiens Spliceosome RNA helicase DDX39B Proteins 0.000 description 1
- 101000707770 Homo sapiens Splicing factor 3B subunit 2 Proteins 0.000 description 1
- 101000861263 Homo sapiens Steroid 21-hydroxylase Proteins 0.000 description 1
- 101000664600 Homo sapiens Tripartite motif-containing protein 3 Proteins 0.000 description 1
- 101000809140 Homo sapiens Uridine-cytidine kinase 1 Proteins 0.000 description 1
- 101000926525 Homo sapiens eIF-2-alpha kinase GCN2 Proteins 0.000 description 1
- 101150017040 I gene Proteins 0.000 description 1
- 102000006496 Immunoglobulin Heavy Chains Human genes 0.000 description 1
- 108010019476 Immunoglobulin Heavy Chains Proteins 0.000 description 1
- 102000013463 Immunoglobulin Light Chains Human genes 0.000 description 1
- 108010065825 Immunoglobulin Light Chains Proteins 0.000 description 1
- 102100036881 Inositol-3-phosphate synthase 1 Human genes 0.000 description 1
- 241000235644 Issatchenkia Species 0.000 description 1
- 229940127379 Kallikrein Inhibitors Drugs 0.000 description 1
- 241001233945 Kazachstania Species 0.000 description 1
- 241001302784 Kodamaea Species 0.000 description 1
- 101100536883 Legionella pneumophila subsp. pneumophila (strain Philadelphia 1 / ATCC 33152 / DSM 7513) thi5 gene Proteins 0.000 description 1
- 241000221479 Leucosporidium Species 0.000 description 1
- 241001508815 Lodderomyces Species 0.000 description 1
- 102100032131 Lymphocyte antigen 6E Human genes 0.000 description 1
- 101150076088 MTD1 gene Proteins 0.000 description 1
- UASDAHIAHBRZQV-YUMQZZPRSA-N Met-Arg Chemical compound CSCC[C@H](N)C(=O)N[C@H](C(O)=O)CCCNC(N)=N UASDAHIAHBRZQV-YUMQZZPRSA-N 0.000 description 1
- 108010006519 Molecular Chaperones Proteins 0.000 description 1
- 102100026101 Molybdopterin-synthase sulfurtransferase Human genes 0.000 description 1
- 108010085220 Multiprotein Complexes Proteins 0.000 description 1
- 102000007474 Multiprotein Complexes Human genes 0.000 description 1
- 241000699666 Mus <mouse, genus> Species 0.000 description 1
- 101000749871 Mus musculus Contactin-associated protein 1 Proteins 0.000 description 1
- 101000931108 Mus musculus DNA (cytosine-5)-methyltransferase 1 Proteins 0.000 description 1
- 241000699670 Mus sp. Species 0.000 description 1
- 102100031871 N-alpha-acetyltransferase 30 Human genes 0.000 description 1
- 102100026873 N-fatty-acyl-amino acid synthase/hydrolase PM20D1 Human genes 0.000 description 1
- 101150045291 NCE103 gene Proteins 0.000 description 1
- 101150039486 NTA1 gene Proteins 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- 108010047956 Nucleosomes Proteins 0.000 description 1
- 241000537797 Ogataea nitratoaversa Species 0.000 description 1
- 108091034117 Oligonucleotide Proteins 0.000 description 1
- 108700026244 Open Reading Frames Proteins 0.000 description 1
- 241000415294 Orectolobidae Species 0.000 description 1
- 101100433758 Oryza sativa subsp. japonica ABCG32 gene Proteins 0.000 description 1
- 101100408299 Oryza sativa subsp. japonica PIL13 gene Proteins 0.000 description 1
- 238000012408 PCR amplification Methods 0.000 description 1
- 101150075113 PFK2 gene Proteins 0.000 description 1
- 101150040663 PGI1 gene Proteins 0.000 description 1
- KJWZYMMLVHIVSU-IYCNHOCDSA-N PGK1 Chemical compound CCCCC[C@H](O)\C=C\[C@@H]1[C@@H](CCCCCCC(O)=O)C(=O)CC1=O KJWZYMMLVHIVSU-IYCNHOCDSA-N 0.000 description 1
- 101150023963 PIL1 gene Proteins 0.000 description 1
- 102100035593 POU domain, class 2, transcription factor 1 Human genes 0.000 description 1
- 101710084414 POU domain, class 2, transcription factor 1 Proteins 0.000 description 1
- 206010033799 Paralysis Diseases 0.000 description 1
- 108091005804 Peptidases Proteins 0.000 description 1
- 102100028960 Peroxisome proliferator-activated receptor gamma coactivator 1-alpha Human genes 0.000 description 1
- 101150018379 Pfk1 gene Proteins 0.000 description 1
- 102100028251 Phosphoglycerate kinase 1 Human genes 0.000 description 1
- 102000004160 Phosphoric Monoester Hydrolases Human genes 0.000 description 1
- 108090000608 Phosphoric Monoester Hydrolases Proteins 0.000 description 1
- 101710132760 Polypyrimidine tract-binding protein 3 Proteins 0.000 description 1
- 102100031243 Polypyrimidine tract-binding protein 3 Human genes 0.000 description 1
- 102100022807 Potassium voltage-gated channel subfamily H member 2 Human genes 0.000 description 1
- 102100026942 Prolyl 3-hydroxylase OGFOD1 Human genes 0.000 description 1
- 239000004365 Protease Substances 0.000 description 1
- 102100020866 Protein SCO1 homolog, mitochondrial Human genes 0.000 description 1
- 101710113900 Protein SGT1 homolog Proteins 0.000 description 1
- 101710166307 Protein lines Proteins 0.000 description 1
- 101150024161 RSB1 gene Proteins 0.000 description 1
- 101710167911 Repressible acid phosphatase Proteins 0.000 description 1
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 description 1
- 241000223252 Rhodotorula Species 0.000 description 1
- 241000221523 Rhodotorula toruloides Species 0.000 description 1
- 108090000621 Ribonuclease P Proteins 0.000 description 1
- 102000004167 Ribonuclease P Human genes 0.000 description 1
- 101150021948 SAM2 gene Proteins 0.000 description 1
- 101150005514 SCS7 gene Proteins 0.000 description 1
- 101150014136 SUC2 gene Proteins 0.000 description 1
- 101100055265 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) ALD2 gene Proteins 0.000 description 1
- 101100340191 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) BNA2 gene Proteins 0.000 description 1
- 101100497534 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) CUB1 gene Proteins 0.000 description 1
- 101100010514 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) DUG3 gene Proteins 0.000 description 1
- 101100236715 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) EHT1 gene Proteins 0.000 description 1
- 101100120627 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) FRD1 gene Proteins 0.000 description 1
- 101100071588 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) HSP12 gene Proteins 0.000 description 1
- 101100507955 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) HXT6 gene Proteins 0.000 description 1
- 101100288519 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) LCB5 gene Proteins 0.000 description 1
- 101100289886 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) LYS4 gene Proteins 0.000 description 1
- 101100133518 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) NNR1 gene Proteins 0.000 description 1
- 101100519256 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) PDR16 gene Proteins 0.000 description 1
- 101100082669 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) PDX3 gene Proteins 0.000 description 1
- 101100407739 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) PET18 gene Proteins 0.000 description 1
- 101100083338 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) PIC2 gene Proteins 0.000 description 1
- 101100451681 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) SSA4 gene Proteins 0.000 description 1
- 101100203981 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) SSP120 gene Proteins 0.000 description 1
- 101100099285 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) THI11 gene Proteins 0.000 description 1
- 101100152887 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) THI3 gene Proteins 0.000 description 1
- 101100482030 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) TPO4 gene Proteins 0.000 description 1
- 101100101631 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) UGA2 gene Proteins 0.000 description 1
- 101100543634 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) YBR056W gene Proteins 0.000 description 1
- 101100212921 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) YBR096W gene Proteins 0.000 description 1
- 101100266867 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) YCR061W gene Proteins 0.000 description 1
- 101100073348 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) YDL124W gene Proteins 0.000 description 1
- 101100159751 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) YEL023C gene Proteins 0.000 description 1
- 101100432033 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) YET3 gene Proteins 0.000 description 1
- 101100267163 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) YHL008C gene Proteins 0.000 description 1
- 101100320522 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) YHR112C gene Proteins 0.000 description 1
- 101100376304 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) YHR140W gene Proteins 0.000 description 1
- 101100320667 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) YIL166C gene Proteins 0.000 description 1
- 101100213747 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) YLR126C gene Proteins 0.000 description 1
- 101100320838 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) YMR244W gene Proteins 0.000 description 1
- 101100488787 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) YNL040W gene Proteins 0.000 description 1
- 101100053305 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) YNL134C gene Proteins 0.000 description 1
- 101100267729 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) YPL225W gene Proteins 0.000 description 1
- 101100488888 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) YPR098C gene Proteins 0.000 description 1
- 241000235344 Saccharomycetaceae Species 0.000 description 1
- 241001149673 Saturnispora Species 0.000 description 1
- 241000235346 Schizosaccharomyces Species 0.000 description 1
- 241000235347 Schizosaccharomyces pombe Species 0.000 description 1
- 101100240664 Schizosaccharomyces pombe (strain 972 / ATCC 24843) nmt1 gene Proteins 0.000 description 1
- 101100063292 Schizosaccharomyces pombe (strain 972 / ATCC 24843) ptp4 gene Proteins 0.000 description 1
- 101100153788 Schizosaccharomyces pombe (strain 972 / ATCC 24843) tpx1 gene Proteins 0.000 description 1
- 241001123650 Schwanniomyces occidentalis Species 0.000 description 1
- 108010003165 Small Nuclear Ribonucleoproteins Proteins 0.000 description 1
- 102000004598 Small Nuclear Ribonucleoproteins Human genes 0.000 description 1
- 102100027722 Small glutamine-rich tetratricopeptide repeat-containing protein alpha Human genes 0.000 description 1
- 102100024690 Spliceosome RNA helicase DDX39B Human genes 0.000 description 1
- 102100031436 Splicing factor 3B subunit 2 Human genes 0.000 description 1
- 241000228389 Sporidiobolus Species 0.000 description 1
- 108091081024 Start codon Proteins 0.000 description 1
- 108010090804 Streptavidin Proteins 0.000 description 1
- 101100029430 Streptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145) pfkA1 gene Proteins 0.000 description 1
- 101100029402 Streptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145) pfkA2 gene Proteins 0.000 description 1
- 101710172711 Structural protein Proteins 0.000 description 1
- 229930006000 Sucrose Natural products 0.000 description 1
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 1
- 241000282887 Suidae Species 0.000 description 1
- 101150048440 TSA1 gene Proteins 0.000 description 1
- 108010017842 Telomerase Proteins 0.000 description 1
- 241000183049 Tetrapisispora Species 0.000 description 1
- 241000235006 Torulaspora Species 0.000 description 1
- 108091023040 Transcription factor Proteins 0.000 description 1
- 102000040945 Transcription factor Human genes 0.000 description 1
- 108700029229 Transcriptional Regulatory Elements Proteins 0.000 description 1
- 108020004566 Transfer RNA Proteins 0.000 description 1
- 241000223259 Trichoderma Species 0.000 description 1
- 102100038798 Tripartite motif-containing protein 3 Human genes 0.000 description 1
- 101100229372 Trypanosoma brucei brucei GK gene Proteins 0.000 description 1
- 102000000523 Type II Activin Receptors Human genes 0.000 description 1
- 108010041546 Type II Activin Receptors Proteins 0.000 description 1
- 101150050575 URA3 gene Proteins 0.000 description 1
- 108091023045 Untranslated Region Proteins 0.000 description 1
- 102100038442 Uridine-cytidine kinase 1 Human genes 0.000 description 1
- 241000251539 Vertebrata <Metazoa> Species 0.000 description 1
- 108020005202 Viral DNA Proteins 0.000 description 1
- 241000235152 Williopsis Species 0.000 description 1
- 108010084455 Zeocin Proteins 0.000 description 1
- 241000235017 Zygosaccharomyces Species 0.000 description 1
- 230000005856 abnormality Effects 0.000 description 1
- 239000012190 activator Substances 0.000 description 1
- 239000000488 activin Substances 0.000 description 1
- 230000001464 adherent effect Effects 0.000 description 1
- 238000005273 aeration Methods 0.000 description 1
- 238000010564 aerobic fermentation Methods 0.000 description 1
- 230000002776 aggregation Effects 0.000 description 1
- 238000004220 aggregation Methods 0.000 description 1
- 101150032697 aim19 gene Proteins 0.000 description 1
- 229960000723 ampicillin Drugs 0.000 description 1
- AVKUERGKIZMTKX-NJBDSQKTSA-N ampicillin Chemical compound C1([C@@H](N)C(=O)N[C@H]2[C@H]3SC([C@@H](N3C2=O)C(O)=O)(C)C)=CC=CC=C1 AVKUERGKIZMTKX-NJBDSQKTSA-N 0.000 description 1
- 229940088710 antibiotic agent Drugs 0.000 description 1
- 230000009830 antibody antigen interaction Effects 0.000 description 1
- 230000000890 antigenic effect Effects 0.000 description 1
- 239000004599 antimicrobial Substances 0.000 description 1
- 238000002617 apheresis Methods 0.000 description 1
- 238000000149 argon plasma sintering Methods 0.000 description 1
- 239000007640 basal medium Substances 0.000 description 1
- 239000011324 bead Substances 0.000 description 1
- 239000003114 blood coagulation factor Substances 0.000 description 1
- 210000004899 c-terminal region Anatomy 0.000 description 1
- 150000001720 carbohydrates Chemical class 0.000 description 1
- 235000014633 carbohydrates Nutrition 0.000 description 1
- 239000003054 catalyst Substances 0.000 description 1
- 230000003197 catalytic effect Effects 0.000 description 1
- 238000004113 cell culture Methods 0.000 description 1
- 230000010261 cell growth Effects 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- 238000007385 chemical modification Methods 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 238000002512 chemotherapy Methods 0.000 description 1
- 230000004186 co-expression Effects 0.000 description 1
- 238000012777 commercial manufacturing Methods 0.000 description 1
- 230000000052 comparative effect Effects 0.000 description 1
- 239000002131 composite material Substances 0.000 description 1
- 239000012141 concentrate Substances 0.000 description 1
- 238000012790 confirmation Methods 0.000 description 1
- 230000001276 controlling effect Effects 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 231100000433 cytotoxic Toxicity 0.000 description 1
- 230000001472 cytotoxic effect Effects 0.000 description 1
- 235000013365 dairy product Nutrition 0.000 description 1
- 101150076739 dbp3 gene Proteins 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000006356 dehydrogenation reaction Methods 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 230000029087 digestion Effects 0.000 description 1
- 230000003467 diminishing effect Effects 0.000 description 1
- 229910001882 dioxygen Inorganic materials 0.000 description 1
- 229940042399 direct acting antivirals protease inhibitors Drugs 0.000 description 1
- 238000009510 drug design Methods 0.000 description 1
- 238000007876 drug discovery Methods 0.000 description 1
- 102100034175 eIF-2-alpha kinase GCN2 Human genes 0.000 description 1
- 108010011867 ecallantide Proteins 0.000 description 1
- 229960001174 ecallantide Drugs 0.000 description 1
- 238000004520 electroporation Methods 0.000 description 1
- 230000002255 enzymatic effect Effects 0.000 description 1
- 108010014507 erythroagglutinating phytohemagglutinin Proteins 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
- 101150017859 exgA gene Proteins 0.000 description 1
- 230000002550 fecal effect Effects 0.000 description 1
- VLMZMRDOMOGGFA-WDBKCZKBSA-N festuclavine Chemical compound C1=CC([C@H]2C[C@H](CN(C)[C@@H]2C2)C)=C3C2=CNC3=C1 VLMZMRDOMOGGFA-WDBKCZKBSA-N 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 239000007850 fluorescent dye Substances 0.000 description 1
- 238000007710 freezing Methods 0.000 description 1
- 230000008014 freezing Effects 0.000 description 1
- 101150073818 gap gene Proteins 0.000 description 1
- 101150112042 gef1 gene Proteins 0.000 description 1
- 238000002523 gelfiltration Methods 0.000 description 1
- 230000004077 genetic alteration Effects 0.000 description 1
- 231100000118 genetic alteration Toxicity 0.000 description 1
- 229930185127 geomycin Natural products 0.000 description 1
- 210000004602 germ cell Anatomy 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 108010051015 glutathione-independent formaldehyde dehydrogenase Proteins 0.000 description 1
- 230000013595 glycosylation Effects 0.000 description 1
- 238000006206 glycosylation reaction Methods 0.000 description 1
- 101150084612 gpmA gene Proteins 0.000 description 1
- 239000003102 growth factor Substances 0.000 description 1
- 238000002744 homologous recombination Methods 0.000 description 1
- 230000006801 homologous recombination Effects 0.000 description 1
- 229920001519 homopolymer Polymers 0.000 description 1
- 102000046768 human CCL2 Human genes 0.000 description 1
- 238000004191 hydrophobic interaction chromatography Methods 0.000 description 1
- 238000003119 immunoblot Methods 0.000 description 1
- 102000028557 immunoglobulin binding proteins Human genes 0.000 description 1
- 108091009323 immunoglobulin binding proteins Proteins 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- 238000006317 isomerization reaction Methods 0.000 description 1
- VBGWSQKGUZHFPS-VGMMZINCSA-N kalbitor Chemical compound C([C@H]1C(=O)N[C@@H](CC(N)=O)C(=O)N[C@H](C(N[C@@H](CC=2C=CC=CC=2)C(=O)N[C@H](C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H]2C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CC=3C=CC=CC=3)C(=O)N[C@H](C(=O)N[C@@H](CC=3C=CC(O)=CC=3)C(=O)NCC(=O)NCC(=O)N[C@H]3CSSC[C@H](NC(=O)[C@@H]4CCCN4C(=O)CNC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CC(O)=O)NC(=O)[C@H](C)NC(=O)[C@H](CCCCN)NC(=O)[C@H](CC=4C=CC=CC=4)NC(=O)[C@H](C)NC(=O)[C@@H](NC(=O)[C@H](CC=4C=CC=CC=4)NC(=O)[C@H](CO)NC(=O)[C@H](CC=4NC=NC=4)NC(=O)[C@H](CCSC)NC(=O)[C@H](C)NC(=O)[C@@H](N)CCC(O)=O)CSSC[C@H](NC(=O)[C@H](CCSC)NC(=O)[C@H](CCCCN)NC(=O)[C@H](CCCCN)NC(=O)[C@@H](NC(=O)[C@H](CCC(O)=O)NC(=O)[C@H](CCC(O)=O)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CO)NC(=O)[C@H](CCC(O)=O)NC(=O)[C@H](CC=4C=CC=CC=4)NC(=O)[C@H](CCCNC(N)=N)NC(=O)[C@H](CC(N)=O)NC(=O)[C@H](CCC(N)=O)NC(=O)[C@H](CC(N)=O)NC(=O)CNC(=O)[C@H](CCC(O)=O)NC3=O)CSSC2)C(=O)N[C@@H]([C@H](C)O)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CC(O)=O)C(O)=O)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](C)C(=O)N[C@@H](C)C(=O)N[C@@H](CC=2NC=NC=2)C(=O)N2CCC[C@H]2C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CC=2C3=CC=CC=C3NC=2)C(=O)N[C@@H](CC=2C=CC=CC=2)C(=O)N1)[C@@H](C)CC)[C@H](C)O)=O)[C@@H](C)CC)C1=CC=CC=C1 VBGWSQKGUZHFPS-VGMMZINCSA-N 0.000 description 1
- 238000011031 large-scale manufacturing process Methods 0.000 description 1
- 230000002045 lasting effect Effects 0.000 description 1
- 239000003446 ligand Substances 0.000 description 1
- 239000002502 liposome Substances 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 230000004807 localization Effects 0.000 description 1
- 101150054280 lys-3 gene Proteins 0.000 description 1
- 101150002475 mae1 gene Proteins 0.000 description 1
- 239000011777 magnesium Substances 0.000 description 1
- 238000012423 maintenance Methods 0.000 description 1
- 108090000280 major urinary proteins Proteins 0.000 description 1
- 102000003888 major urinary proteins Human genes 0.000 description 1
- FYGDTMLNYKFZSV-UHFFFAOYSA-N mannotriose Natural products OC1C(O)C(O)C(CO)OC1OC1C(CO)OC(OC2C(OC(O)C(O)C2O)CO)C(O)C1O FYGDTMLNYKFZSV-UHFFFAOYSA-N 0.000 description 1
- 238000000691 measurement method Methods 0.000 description 1
- 230000001404 mediated effect Effects 0.000 description 1
- 239000013028 medium composition Substances 0.000 description 1
- 230000021121 meiosis Effects 0.000 description 1
- VUZPPFZMUPKLLV-UHFFFAOYSA-N methane;hydrate Chemical compound C.O VUZPPFZMUPKLLV-UHFFFAOYSA-N 0.000 description 1
- 229940057059 monascus purpureus Drugs 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- 231100000219 mutagenic Toxicity 0.000 description 1
- 230000003505 mutagenic effect Effects 0.000 description 1
- 231100000252 nontoxic Toxicity 0.000 description 1
- 230000003000 nontoxic effect Effects 0.000 description 1
- 210000001623 nucleosome Anatomy 0.000 description 1
- 239000002773 nucleotide Substances 0.000 description 1
- 125000003729 nucleotide group Chemical group 0.000 description 1
- 230000003287 optical effect Effects 0.000 description 1
- 230000003204 osmotic effect Effects 0.000 description 1
- 230000036961 partial effect Effects 0.000 description 1
- 230000037361 pathway Effects 0.000 description 1
- 239000000137 peptide hydrolase inhibitor Substances 0.000 description 1
- 230000002085 persistent effect Effects 0.000 description 1
- CWCMIVBLVUHDHK-ZSNHEYEWSA-N phleomycin D1 Chemical compound N([C@H](C(=O)N[C@H](C)[C@@H](O)[C@H](C)C(=O)N[C@@H]([C@H](O)C)C(=O)NCCC=1SC[C@@H](N=1)C=1SC=C(N=1)C(=O)NCCCCNC(N)=N)[C@@H](O[C@H]1[C@H]([C@@H](O)[C@H](O)[C@H](CO)O1)O[C@@H]1[C@H]([C@@H](OC(N)=O)[C@H](O)[C@@H](CO)O1)O)C=1N=CNC=1)C(=O)C1=NC([C@H](CC(N)=O)NC[C@H](N)C(N)=O)=NC(N)=C1C CWCMIVBLVUHDHK-ZSNHEYEWSA-N 0.000 description 1
- 230000026731 phosphorylation Effects 0.000 description 1
- 238000006366 phosphorylation reaction Methods 0.000 description 1
- 230000001766 physiological effect Effects 0.000 description 1
- 101150088709 plb3 gene Proteins 0.000 description 1
- 230000008488 polyadenylation Effects 0.000 description 1
- 238000003752 polymerase chain reaction Methods 0.000 description 1
- 210000001850 polyploid cell Anatomy 0.000 description 1
- 238000001556 precipitation Methods 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- PUIBPGHAXSCVRF-QHFGJBOXSA-N prostaglandin C1 Chemical compound CCCCC[C@H](O)\C=C\C1=CCC(=O)[C@@H]1CCCCCCC(O)=O PUIBPGHAXSCVRF-QHFGJBOXSA-N 0.000 description 1
- 230000012846 protein folding Effects 0.000 description 1
- 239000012857 radioactive material Substances 0.000 description 1
- 230000008707 rearrangement Effects 0.000 description 1
- 108020003175 receptors Proteins 0.000 description 1
- 102000005962 receptors Human genes 0.000 description 1
- 230000001850 reproductive effect Effects 0.000 description 1
- 210000003705 ribosome Anatomy 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 239000006152 selective media Substances 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 238000012163 sequencing technique Methods 0.000 description 1
- 210000002966 serum Anatomy 0.000 description 1
- 238000001542 size-exclusion chromatography Methods 0.000 description 1
- 150000003384 small molecules Chemical class 0.000 description 1
- 239000001488 sodium phosphate Substances 0.000 description 1
- 229910000162 sodium phosphate Inorganic materials 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 230000000392 somatic effect Effects 0.000 description 1
- 230000009870 specific binding Effects 0.000 description 1
- 230000006641 stabilisation Effects 0.000 description 1
- 238000011105 stabilization Methods 0.000 description 1
- 230000000087 stabilizing effect Effects 0.000 description 1
- 238000010561 standard procedure Methods 0.000 description 1
- 239000007858 starting material Substances 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 239000005720 sucrose Substances 0.000 description 1
- 150000008163 sugars Chemical class 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
- 230000002194 synthesizing effect Effects 0.000 description 1
- 230000009897 systematic effect Effects 0.000 description 1
- 101150088047 tdh3 gene Proteins 0.000 description 1
- 238000010257 thawing Methods 0.000 description 1
- 229940124597 therapeutic agent Drugs 0.000 description 1
- 229940126622 therapeutic monoclonal antibody Drugs 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
- 108091006106 transcriptional activators Proteins 0.000 description 1
- 238000000844 transformation Methods 0.000 description 1
- 230000014621 translational initiation Effects 0.000 description 1
- RYFMWSXOAZQYPI-UHFFFAOYSA-K trisodium phosphate Chemical compound [Na+].[Na+].[Na+].[O-]P([O-])([O-])=O RYFMWSXOAZQYPI-UHFFFAOYSA-K 0.000 description 1
- 238000000825 ultraviolet detection Methods 0.000 description 1
- 238000011144 upstream manufacturing Methods 0.000 description 1
- 108700026220 vif Genes Proteins 0.000 description 1
- 108010027345 wheylin-1 peptide Proteins 0.000 description 1
- 101150061553 zrt1 gene Proteins 0.000 description 1
- FYGDTMLNYKFZSV-BYLHFPJWSA-N β-1,4-galactotrioside Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1O[C@@H]1[C@H](CO)O[C@@H](O[C@@H]2[C@@H](O[C@@H](O)[C@H](O)[C@H]2O)CO)[C@H](O)[C@H]1O FYGDTMLNYKFZSV-BYLHFPJWSA-N 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
- C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
- C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
- C07K16/24—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against cytokines, lymphokines or interferons
- C07K16/244—Interleukins [IL]
- C07K16/248—IL-6
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/16—Yeasts; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/10—Immunoglobulins specific features characterized by their source of isolation or production
- C07K2317/14—Specific host cells or culture conditions, e.g. components, pH or temperature
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/20—Immunoglobulins specific features characterized by taxonomic origin
- C07K2317/24—Immunoglobulins specific features characterized by taxonomic origin containing regions, domains or residues from different species, e.g. chimeric, humanized or veneered
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
- C07K2317/56—Immunoglobulins specific features characterized by immunoglobulin fragments variable (Fv) region, i.e. VH and/or VL
- C07K2317/565—Complementarity determining region [CDR]
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Molecular Biology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Medicinal Chemistry (AREA)
- Microbiology (AREA)
- General Engineering & Computer Science (AREA)
- Biophysics (AREA)
- Immunology (AREA)
- Mycology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Tropical Medicine & Parasitology (AREA)
- Virology (AREA)
- Biomedical Technology (AREA)
- Botany (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Analytical Chemistry (AREA)
- Enzymes And Modification Thereof (AREA)
Applications Claiming Priority (4)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201361790613P | 2013-03-15 | 2013-03-15 | |
| US201361791471P | 2013-03-15 | 2013-03-15 | |
| US61/791,471 | 2013-03-15 | ||
| US61/790,613 | 2013-03-15 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| TW201512401A TW201512401A (zh) | 2015-04-01 |
| TWI636136B true TWI636136B (zh) | 2018-09-21 |
Family
ID=51538058
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| TW103109931A TWI636136B (zh) | 2013-03-15 | 2014-03-17 | 在酵母菌及其它轉型細胞中多肽高產量表現用之溫度變動技術 |
Country Status (15)
| Country | Link |
|---|---|
| US (2) | US10138294B2 (cg-RX-API-DMAC7.html) |
| EP (2) | EP3597664B1 (cg-RX-API-DMAC7.html) |
| JP (1) | JP6412552B2 (cg-RX-API-DMAC7.html) |
| KR (1) | KR102202477B1 (cg-RX-API-DMAC7.html) |
| CN (1) | CN105121652B (cg-RX-API-DMAC7.html) |
| AU (1) | AU2014232813B2 (cg-RX-API-DMAC7.html) |
| CA (1) | CA2905180C (cg-RX-API-DMAC7.html) |
| DK (1) | DK2971037T3 (cg-RX-API-DMAC7.html) |
| ES (1) | ES2749751T3 (cg-RX-API-DMAC7.html) |
| IL (1) | IL241176B (cg-RX-API-DMAC7.html) |
| MX (2) | MX366647B (cg-RX-API-DMAC7.html) |
| SG (1) | SG11201506034TA (cg-RX-API-DMAC7.html) |
| TW (1) | TWI636136B (cg-RX-API-DMAC7.html) |
| WO (1) | WO2014145650A1 (cg-RX-API-DMAC7.html) |
| ZA (1) | ZA201505483B (cg-RX-API-DMAC7.html) |
Families Citing this family (20)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US10202630B2 (en) * | 2013-03-15 | 2019-02-12 | Alderbio Holdings Llc | Temperature shift for high yield expression of polypeptides in yeast and other transformed cells |
| US10556945B2 (en) | 2014-03-21 | 2020-02-11 | Teva Pharmaceuticals International Gmbh | Antagonist antibodies directed against calcitonin gene-related peptide and methods using same |
| CN111973740A (zh) | 2014-03-21 | 2020-11-24 | 泰华制药国际有限公司 | 针对降钙素基因相关肽的拮抗剂抗体及其使用方法 |
| US11518797B2 (en) | 2014-11-11 | 2022-12-06 | Clara Foods Co. | Methods and compositions for egg white protein production |
| JP2017045865A (ja) | 2015-08-26 | 2017-03-02 | ルネサスエレクトロニクス株式会社 | 半導体装置およびその製造方法 |
| CN106191181B (zh) | 2016-07-22 | 2019-12-13 | 江苏江山聚源生物技术有限公司 | 一种毕赤酵母表达重组蛋白的发酵工艺 |
| CN109952314A (zh) | 2016-09-23 | 2019-06-28 | 泰瓦制药国际有限公司 | 治疗难治性偏头痛 |
| KR102638074B1 (ko) | 2017-03-10 | 2024-02-20 | 볼트 쓰레즈, 인크. | 재조합 단백질을 고분비 수율로 생산하기 위한 조성물 및 방법 |
| JP7063486B2 (ja) | 2017-03-10 | 2022-05-09 | ボルト スレッズ インコーポレイテッド | 高分泌収量の組み換えタンパク質を産生するための組成物及び方法 |
| KR20250016476A (ko) * | 2018-03-16 | 2025-02-03 | 젠자임 코포레이션 | 생산용 생물 반응기에서의 세포 생존력을 개선하는 방법 |
| US10221403B1 (en) * | 2018-04-25 | 2019-03-05 | Life Rainbow Biotech Co., Ltd. | Method of preparing zearalenone hydrolase |
| CN108546637A (zh) * | 2018-05-23 | 2018-09-18 | 华东理工大学 | 重组毕赤酵母表达葡萄糖氧化酶发酵装置以及发酵培养方法 |
| EP3821022A1 (en) * | 2018-07-12 | 2021-05-19 | Amyris, Inc. | Methods for controlling fermentation feed rates |
| CA3146649A1 (en) | 2019-07-11 | 2021-01-14 | Clara Foods Co. | Protein compositions and consumable products thereof |
| US12096784B2 (en) | 2019-07-11 | 2024-09-24 | Clara Foods Co. | Protein compositions and consumable products thereof |
| US10927360B1 (en) | 2019-08-07 | 2021-02-23 | Clara Foods Co. | Compositions comprising digestive enzymes |
| WO2021092356A1 (en) * | 2019-11-08 | 2021-05-14 | Danisco Us Inc | Fungal strains comprising enhanced protein productivity phenotypes and methods thereof |
| US20230074785A1 (en) * | 2020-01-14 | 2023-03-09 | Basf Se | Method For Producing Recombinant Protein In Yeast Cells |
| US20250207158A1 (en) * | 2022-03-24 | 2025-06-26 | Versalis S.P.A. | Fermentation process for the production of lipids from oleaginous yeasts |
| WO2024073755A2 (en) * | 2022-09-29 | 2024-04-04 | Antheia, Inc. | Methods of improving production of morphinan alkaloids and derivatives |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6221630B1 (en) * | 1999-03-24 | 2001-04-24 | The Penn State Research Foundation | High copy number recombinant expression construct for regulated high-level production of polypeptides in yeast |
Family Cites Families (22)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4855231A (en) | 1984-10-30 | 1989-08-08 | Phillips Petroleum Company | Regulatory region for heterologous gene expression in yeast |
| US4818700A (en) | 1985-10-25 | 1989-04-04 | Phillips Petroleum Company | Pichia pastoris argininosuccinate lyase gene and uses thereof |
| US4812405A (en) | 1986-02-18 | 1989-03-14 | Phillips Petroleum Company | Double auxotrophic mutants of Pichia pastoris and methods for preparation |
| US4929555A (en) | 1987-10-19 | 1990-05-29 | Phillips Petroleum Company | Pichia transformation |
| WO1994021293A1 (en) | 1993-03-19 | 1994-09-29 | Duke University | Method of treatment of tumors with an antibody binding to tenascin |
| ATE306930T1 (de) | 1994-08-12 | 2005-11-15 | Immunomedics Inc | Für b-zell-lymphom und leukämiezellen spezifische immunkonjugate und humane antikörper |
| US5955349A (en) | 1996-08-26 | 1999-09-21 | Zymogenetics, Inc. | Compositions and methods for producing heterologous polypeptides in Pichia methanolica |
| US5736383A (en) | 1996-08-26 | 1998-04-07 | Zymogenetics, Inc. | Preparation of Pichia methanolica auxotrophic mutants |
| US6730499B1 (en) | 1998-07-03 | 2004-05-04 | Research Corporation Technologies, Inc. | Promoter for the Pichia pastoris formaldehyde dehydrogenase gene FLD1 |
| US6258559B1 (en) | 1999-03-22 | 2001-07-10 | Zymogenetics, Inc. | Method for producing proteins in transformed Pichia |
| NZ547279A (en) | 2003-10-22 | 2008-04-30 | Keck Graduate Inst | Methods of synthesizing heteromultimeric polypeptides in yeast using a haploid mating strategy |
| EP2021463B1 (en) | 2006-05-19 | 2016-11-23 | Alder Biopharmaceuticals, Inc. | Culture method for obtaining a clonal population of antigen-specific b cells |
| CN105001332B (zh) | 2007-05-21 | 2018-12-04 | 奥尔德生物控股有限责任公司 | 针对il-6的抗体及其用途 |
| EP2162469A4 (en) | 2007-05-21 | 2012-08-01 | Alderbio Holdings Llc | NEW METHODS OF HUMANIZING RABBIT ANTIBODIES AND HUMANIZED RABBIT ANTIBODIES |
| US20090269832A1 (en) * | 2007-11-05 | 2009-10-29 | Modular Genetics, Inc. | Growth of Microorganisms in Media Containing Crude Glycerol |
| US8501449B2 (en) * | 2007-12-04 | 2013-08-06 | Proteon Therapeutics, Inc. | Recombinant elastase proteins and methods of manufacturing and use thereof |
| WO2011075686A2 (en) * | 2009-12-18 | 2011-06-23 | Ventria Bioscience | Methods & compositions comprising heat shock proteins |
| US11214610B2 (en) | 2010-12-01 | 2022-01-04 | H. Lundbeck A/S | High-purity production of multi-subunit proteins such as antibodies in transformed microbes such as Pichia pastoris |
| EP2744903B1 (en) * | 2011-08-19 | 2018-10-10 | AlderBio Holdings LLC | Multi-copy strategy for high-titer and high-purity production of multi-subunit proteins such as a antibodies in transformed microbes such as pichia pastoris |
| US10150968B2 (en) | 2011-08-19 | 2018-12-11 | Alderbio Holdings Llc | Multi-copy strategy for high-titer and high-purity production of multi-subunit proteins such as antibodies in transformed microbes such as Pichia pastoris |
| WO2014145521A2 (en) * | 2013-03-15 | 2014-09-18 | Alderbio Holdings Llc | Fermentation process for antibody production |
| US10202630B2 (en) * | 2013-03-15 | 2019-02-12 | Alderbio Holdings Llc | Temperature shift for high yield expression of polypeptides in yeast and other transformed cells |
-
2014
- 2014-03-17 MX MX2015011968A patent/MX366647B/es active IP Right Grant
- 2014-03-17 EP EP19177519.6A patent/EP3597664B1/en active Active
- 2014-03-17 MX MX2019008417A patent/MX384491B/es unknown
- 2014-03-17 US US14/776,892 patent/US10138294B2/en active Active
- 2014-03-17 DK DK14764393.6T patent/DK2971037T3/da active
- 2014-03-17 JP JP2016503404A patent/JP6412552B2/ja active Active
- 2014-03-17 EP EP14764393.6A patent/EP2971037B1/en active Active
- 2014-03-17 CN CN201480014647.8A patent/CN105121652B/zh active Active
- 2014-03-17 KR KR1020157028401A patent/KR102202477B1/ko active Active
- 2014-03-17 SG SG11201506034TA patent/SG11201506034TA/en unknown
- 2014-03-17 WO PCT/US2014/030453 patent/WO2014145650A1/en not_active Ceased
- 2014-03-17 CA CA2905180A patent/CA2905180C/en active Active
- 2014-03-17 AU AU2014232813A patent/AU2014232813B2/en active Active
- 2014-03-17 TW TW103109931A patent/TWI636136B/zh active
- 2014-03-17 ES ES14764393T patent/ES2749751T3/es active Active
-
2015
- 2015-07-30 ZA ZA2015/05483A patent/ZA201505483B/en unknown
- 2015-09-03 IL IL241176A patent/IL241176B/en active IP Right Grant
-
2018
- 2018-10-24 US US16/169,130 patent/US11447540B2/en active Active
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6221630B1 (en) * | 1999-03-24 | 2001-04-24 | The Penn State Research Foundation | High copy number recombinant expression construct for regulated high-level production of polypeptides in yeast |
Also Published As
| Publication number | Publication date |
|---|---|
| EP3597664B1 (en) | 2025-11-26 |
| EP2971037A4 (en) | 2016-07-20 |
| IL241176B (en) | 2020-05-31 |
| AU2014232813B2 (en) | 2017-12-07 |
| MX384491B (es) | 2025-03-14 |
| KR20150140678A (ko) | 2015-12-16 |
| CN105121652B (zh) | 2020-07-28 |
| TW201512401A (zh) | 2015-04-01 |
| EP2971037A1 (en) | 2016-01-20 |
| ES2749751T3 (es) | 2020-03-23 |
| KR102202477B1 (ko) | 2021-01-13 |
| NZ711400A (en) | 2021-05-28 |
| AU2014232813A1 (en) | 2015-09-03 |
| US20190119360A1 (en) | 2019-04-25 |
| ZA201505483B (en) | 2022-08-31 |
| CN105121652A (zh) | 2015-12-02 |
| SG11201506034TA (en) | 2015-09-29 |
| US10138294B2 (en) | 2018-11-27 |
| DK2971037T3 (da) | 2019-10-14 |
| EP2971037B1 (en) | 2019-07-10 |
| US11447540B2 (en) | 2022-09-20 |
| EP3597664A2 (en) | 2020-01-22 |
| MX2015011968A (es) | 2017-01-06 |
| CA2905180C (en) | 2023-03-07 |
| JP6412552B2 (ja) | 2018-10-24 |
| MX366647B (es) | 2019-07-17 |
| CA2905180A1 (en) | 2014-09-18 |
| MX2019008417A (es) | 2019-09-13 |
| US20160039911A1 (en) | 2016-02-11 |
| IL241176A0 (en) | 2015-11-30 |
| EP3597664A3 (en) | 2020-03-11 |
| JP2016516412A (ja) | 2016-06-09 |
| WO2014145650A1 (en) | 2014-09-18 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| TWI636136B (zh) | 在酵母菌及其它轉型細胞中多肽高產量表現用之溫度變動技術 | |
| JP6138788B2 (ja) | 形質転換した微生物、例えばピキア・パストリス中で抗体などのマルチサブユニットタンパク質を高濃度かつ高純度で生産するための多コピー法 | |
| US11225667B2 (en) | Multi-copy strategy for high-titer and high-purity production of multi-subunit proteins such as antibodies in transformed microbes such as Pichia pastoris | |
| JP6491696B2 (ja) | 形質転換されたピキア・パストリス（Ｐｉｃｈｉａ ｐａｓｔｏｒｉｓ）などの微生物における抗体などのマルチサブユニットタンパク質の高純度生産 | |
| US12077794B2 (en) | Temperature shift for high yield expression of polypeptides in yeast and other transformed cells | |
| HK40020901A (en) | Temperature shift for high yield expression of polypeptides in yeast and other transformed cells | |
| NZ711400B2 (en) | Temperature shift for high yield expression of polypeptides in yeast and other transformed cells | |
| NZ621199B2 (en) | Multi-copy strategy for high-titer and high-purity production of multi-subunit proteins such as antibodies in transformed microbes such as pichia pastoris | |
| NZ721102B2 (en) | Multi-copy strategy for high-titer and high-purity production of multi-subunit proteins such as antibodies in transformed microbes such as pichia pastoris |