TWI583788B - 細胞培養之預程式化無回饋控制之連續進料 - Google Patents
細胞培養之預程式化無回饋控制之連續進料 Download PDFInfo
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- TWI583788B TWI583788B TW101133866A TW101133866A TWI583788B TW I583788 B TWI583788 B TW I583788B TW 101133866 A TW101133866 A TW 101133866A TW 101133866 A TW101133866 A TW 101133866A TW I583788 B TWI583788 B TW I583788B
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- Prior art keywords
- feed
- continuous
- cell culture
- glucose
- cell
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Links
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- 238000000034 method Methods 0.000 claims description 125
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- 239000008103 glucose Substances 0.000 claims description 110
- 235000015097 nutrients Nutrition 0.000 claims description 70
- 230000012010 growth Effects 0.000 claims description 43
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 claims description 18
- 210000004962 mammalian cell Anatomy 0.000 claims description 15
- 150000001413 amino acids Chemical class 0.000 claims description 14
- 235000014655 lactic acid Nutrition 0.000 claims description 9
- 239000004310 lactic acid Substances 0.000 claims description 9
- 230000003204 osmotic effect Effects 0.000 claims description 7
- 238000006243 chemical reaction Methods 0.000 claims description 4
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- 235000006286 nutrient intake Nutrition 0.000 claims description 4
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- 108010011485 Aspartame Proteins 0.000 claims description 2
- IAOZJIPTCAWIRG-QWRGUYRKSA-N aspartame Chemical compound OC(=O)C[C@H](N)C(=O)N[C@H](C(=O)OC)CC1=CC=CC=C1 IAOZJIPTCAWIRG-QWRGUYRKSA-N 0.000 claims description 2
- 239000000605 aspartame Substances 0.000 claims description 2
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- 125000002791 glucosyl group Chemical group C1([C@H](O)[C@@H](O)[C@H](O)[C@H](O1)CO)* 0.000 claims description 2
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- 108020004707 nucleic acids Proteins 0.000 description 1
- 102000039446 nucleic acids Human genes 0.000 description 1
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- 239000001301 oxygen Substances 0.000 description 1
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- 235000019319 peptone Nutrition 0.000 description 1
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
- C12M41/32—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of substances in solution
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
- C12M41/38—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of metabolites or enzymes in the cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/40—Means for regulation, monitoring, measurement or control, e.g. flow regulation of pressure
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Microbiology (AREA)
- Genetics & Genomics (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Sustainable Development (AREA)
- Analytical Chemistry (AREA)
- Cell Biology (AREA)
- Molecular Biology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201161535809P | 2011-09-16 | 2011-09-16 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| TW201326387A TW201326387A (zh) | 2013-07-01 |
| TWI583788B true TWI583788B (zh) | 2017-05-21 |
Family
ID=46964067
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| TW101133866A TWI583788B (zh) | 2011-09-16 | 2012-09-14 | 細胞培養之預程式化無回饋控制之連續進料 |
Country Status (16)
| Country | Link |
|---|---|
| US (1) | US9803166B2 (https=) |
| EP (1) | EP2756069B1 (https=) |
| JP (4) | JP2014526265A (https=) |
| KR (1) | KR101710766B1 (https=) |
| CN (1) | CN103946366B (https=) |
| AU (1) | AU2012308335A1 (https=) |
| BR (1) | BR112014008303A2 (https=) |
| CA (1) | CA2846905A1 (https=) |
| EA (1) | EA201490502A1 (https=) |
| ES (1) | ES2862576T3 (https=) |
| IL (1) | IL231032A0 (https=) |
| MX (1) | MX356956B (https=) |
| SG (1) | SG11201400656SA (https=) |
| TW (1) | TWI583788B (https=) |
| WO (1) | WO2013040444A1 (https=) |
| ZA (1) | ZA201401655B (https=) |
Families Citing this family (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AU765203B2 (en) | 1999-04-19 | 2003-09-11 | Shionogi & Co., Ltd. | Sulfonamide derivatives having oxadiazole rings |
| AU2015269365A1 (en) | 2014-06-04 | 2016-12-15 | Amgen Inc. | Methods for harvesting mammalian cell cultures |
| PL3865563T3 (pl) | 2014-11-25 | 2024-01-29 | Corning Incorporated | Materiały i sposoby do przedłużania pożywki do hodowli komórkowej |
| CA2969225C (en) | 2014-12-01 | 2023-08-22 | Amgen Inc. | Process for manipulating the level of glycan content of a glycoprotein |
| WO2016183016A1 (en) * | 2015-05-08 | 2016-11-17 | Flownamics Analytical Instruments, Inc. | Method & apparatus for continuous automated perfusion system harvesting from in-situ filtration probe |
| WO2021097281A1 (en) | 2019-11-15 | 2021-05-20 | Lonza Ltd | Process and system for producing an inoculum |
| US20230287335A1 (en) | 2020-07-30 | 2023-09-14 | Amgen Inc. | Cell culture media and methods of making and using the same |
| CN112553044A (zh) * | 2020-12-10 | 2021-03-26 | 上海奥浦迈生物科技股份有限公司 | 一种生物反应器细胞培养进样口用防堵塞装置 |
| KR20250056246A (ko) * | 2022-10-13 | 2025-04-25 | 가부시키가이샤 히타치세이사쿠쇼 | 배양 장치의 제어 방법, 및 세포 배양을 수반하는 배양 장치의 제어 방법 |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE102004007658A1 (de) * | 2004-02-17 | 2005-09-01 | Bioceuticals Arzneimittel Ag | Verfahren zur Herstellung von rekombinanten Proteinen in eukaryontischen Wirtszellen |
Family Cites Families (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5629202A (en) | 1994-07-19 | 1997-05-13 | Development Center For Biotechnology | Computer-controlled bioreactor system for enzymatic synthesis of L-tryptophan |
| ATE241695T1 (de) * | 1996-03-13 | 2003-06-15 | Delta Biotechnology Ltd | Gärungs-steuerungssysteme |
| WO1998041611A1 (en) | 1997-03-20 | 1998-09-24 | Regents Of The University Of Minnesota | Process for the continuous culture of cells |
| CN1678731A (zh) * | 2002-08-27 | 2005-10-05 | 范德比尔特大学 | 多室生物反应器 |
| CN1778903A (zh) | 2005-09-29 | 2006-05-31 | 华东理工大学 | 一种动物细胞高密度连续灌注培养方法 |
| WO2007071072A1 (en) * | 2005-12-22 | 2007-06-28 | Corporation De L'ecole Polytechnique De Montreal | High-rate perfusion bioreactor |
| JP2007202500A (ja) * | 2006-02-03 | 2007-08-16 | Hitachi Ltd | 培養槽の運転制御装置 |
| WO2008136398A1 (ja) | 2007-04-26 | 2008-11-13 | Chugai Seiyaku Kabushiki Kaisha | 高濃度アミノ酸含有培地を用いた細胞の培養方法 |
-
2012
- 2012-09-14 TW TW101133866A patent/TWI583788B/zh active
- 2012-09-14 MX MX2014003134A patent/MX356956B/es active IP Right Grant
- 2012-09-14 EA EA201490502A patent/EA201490502A1/ru unknown
- 2012-09-14 AU AU2012308335A patent/AU2012308335A1/en not_active Abandoned
- 2012-09-14 KR KR1020147005482A patent/KR101710766B1/ko active Active
- 2012-09-14 ES ES12766802T patent/ES2862576T3/es active Active
- 2012-09-14 JP JP2014530890A patent/JP2014526265A/ja active Pending
- 2012-09-14 SG SG11201400656SA patent/SG11201400656SA/en unknown
- 2012-09-14 BR BR112014008303A patent/BR112014008303A2/pt not_active IP Right Cessation
- 2012-09-14 EP EP12766802.8A patent/EP2756069B1/en active Active
- 2012-09-14 WO PCT/US2012/055552 patent/WO2013040444A1/en not_active Ceased
- 2012-09-14 CN CN201280044589.4A patent/CN103946366B/zh active Active
- 2012-09-14 CA CA2846905A patent/CA2846905A1/en not_active Abandoned
- 2012-09-14 US US14/342,304 patent/US9803166B2/en active Active
-
2014
- 2014-02-18 IL IL231032A patent/IL231032A0/en unknown
- 2014-03-05 ZA ZA2014/01655A patent/ZA201401655B/en unknown
-
2016
- 2016-09-30 JP JP2016193713A patent/JP2017029162A/ja active Pending
-
2019
- 2019-10-24 JP JP2019193185A patent/JP2020022492A/ja active Pending
-
2022
- 2022-01-21 JP JP2022007644A patent/JP2022058710A/ja active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE102004007658A1 (de) * | 2004-02-17 | 2005-09-01 | Bioceuticals Arzneimittel Ag | Verfahren zur Herstellung von rekombinanten Proteinen in eukaryontischen Wirtszellen |
Non-Patent Citations (1)
| Title |
|---|
| 劉興茂，｢CHO工程細胞無血清流加培養代謝動力學｣，中國軍事醫學科學院生物工程研究所博士論文，2009年第9期 * |
Also Published As
| Publication number | Publication date |
|---|---|
| WO2013040444A1 (en) | 2013-03-21 |
| AU2012308335A1 (en) | 2014-03-13 |
| JP2022058710A (ja) | 2022-04-12 |
| EA201490502A1 (ru) | 2014-06-30 |
| IL231032A0 (en) | 2014-03-31 |
| MX356956B (es) | 2018-06-21 |
| CN103946366B (zh) | 2017-08-11 |
| SG11201400656SA (en) | 2014-04-28 |
| BR112014008303A2 (pt) | 2017-04-11 |
| ES2862576T3 (es) | 2021-10-07 |
| KR101710766B1 (ko) | 2017-02-27 |
| TW201326387A (zh) | 2013-07-01 |
| KR20140044396A (ko) | 2014-04-14 |
| US20140295545A1 (en) | 2014-10-02 |
| US9803166B2 (en) | 2017-10-31 |
| EP2756069A1 (en) | 2014-07-23 |
| JP2020022492A (ja) | 2020-02-13 |
| JP2014526265A (ja) | 2014-10-06 |
| EP2756069B1 (en) | 2021-01-27 |
| MX2014003134A (es) | 2014-04-25 |
| JP2017029162A (ja) | 2017-02-09 |
| CA2846905A1 (en) | 2013-03-21 |
| ZA201401655B (en) | 2014-12-23 |
| CN103946366A (zh) | 2014-07-23 |
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